Disclosure of Invention
To solve the above technical problems, the present invention aims to: the device and the method for detecting plasma chyle and hemolysis are high in practicability and accuracy and suitable for test tubes of multiple specifications.
On one hand, the technical scheme adopted by the invention is as follows:
the detection device is suitable for plasma chyle and hemolysis of test tubes of various specifications and comprises a base, wherein a signal processing system, a power supply and a motor are arranged in the base, a plurality of test tube lattices are arranged on the base, hard plastic shells are arranged on the test tube lattices, a color sensor is arranged on the side wall or the rear wall of each hard plastic shell, a fixing force wheel is arranged above the color sensor, a power wheel is arranged below the color sensor, the fixing force wheel is connected with the power wheel through double chains, and the color sensor is positioned between the double chains;
the color sensor is used for acquiring color sensing signals of a blood sample in a test tube placed in the test tube lattice in real time;
the signal processing system is used for generating a detection result of the chyle degree and the hemolysis degree of the plasma according to the blood color sensing signal; triggering a corresponding control signal, and then sending the control signal to the motor;
the motor is used for rotating by a corresponding amplitude according to a control signal of the signal processing system so as to drive the power wheel to rotate by a corresponding number of turns, so that the height position of the color sensor is adjusted;
and the power supply is used for providing working power supply signals for the signal processing system, the motor and the color sensor.
Further, a side wall distance sensor is arranged on one side of the inner side wall of the hard plastic shell;
the side wall distance sensor is used for measuring the distance between the side wall of the inner side of the rigid plastic shell and the side wall of the test tube.
Further, a bottom distance sensor is arranged at the bottom of the hard plastic shell;
the bottom distance sensor is used for measuring the distance between the bottom of the inner side of the hard plastic shell and the bottom of the test tube.
Furthermore, a gravity sensor is also arranged on the hard plastic shell;
wherein, the gravity inductor is used for acquiring the gravity signal of the test tube in the test tube lattice in real time.
The technical scheme adopted by the other aspect of the invention is as follows:
the method of the plasma chyle and hemolysis detection device suitable for the multi-specification test tubes comprises the following steps:
acquiring a color sensing signal of a blood sample in a test tube placed in a test tube lattice in real time through a color sensor;
triggering a corresponding control signal through a signal processing system according to the blood color sensing signal, and sending the control signal to the motor;
the motor rotates by a corresponding amplitude according to a control signal of the signal processing system, and then drives the power wheel to rotate by a corresponding number of turns so as to realize the height position adjustment of the color sensor;
the signal processing system, the motor and the color sensor are provided with working power supply signals through a power supply.
Further, the method also comprises the following steps:
and measuring the distance between the inner side wall of the rigid plastic shell and the side wall of the test tube by using a side wall distance sensor.
Further, the method also comprises the following steps:
and measuring the distance between the bottom of the inner side of the hard plastic shell and the bottom of the test tube by using a bottom distance sensor.
Further, the method also comprises the following steps:
and acquiring the gravity signal of the test tube in the test tube lattice in real time through a gravity sensor.
Further, the method also comprises the following steps:
calculating the mass of the blood according to the gravity of the empty test tube and the gravity of the centrifuged test tube;
calculating the volume of blood based on the mass of blood;
calculating the diameter of the test tube according to the distance between the inner side wall of the rigid plastic shell and the side wall of the test tube and the diameter of the rigid plastic shell;
calculating the height of the blood liquid level in the test tube according to the diameter of the test tube;
calculating the height of the liquid level of the plasma and the height of the midpoint of the plasma according to the height of the liquid level of the blood and the distance between the bottom of the inner side of the hard plastic shell and the bottom of the test tube;
and adjusting the height position of the color sensor according to the plasma liquid level height and the plasma midpoint height.
Further, the step of adjusting the height position of the color sensor according to the plasma liquid level height and the plasma midpoint height comprises the following steps:
acquiring an initial starting position of a color sensor, and calculating the distance between the initial starting position and the bottom of the hard plastic shell;
calculating the moving height of the color sensor according to the distance between the bottom of the inner side of the hard plastic shell and the bottom of the test tube, the height of the blood liquid level and the distance between the initial starting position and the bottom of the hard plastic shell;
calculating the number of turns of the power wheel according to the moving height of the color sensor;
generating a working control signal of the motor through a signal processing system according to the rotating number of turns of the power wheel;
and a signal processing system sends a working control signal to the motor to control the motor to rotate so as to adjust the height position of the color sensor.
The invention has the beneficial effects that: the device is additionally provided with the color sensor, so that the color signal of the plasma in the test tube can be obtained in real time, and the detection result of plasma chyle and hemolysis is obtained by matching with a signal processing system, so that the accuracy is high; in addition, the motor starts to work according to the control signal of the signal processing system, so that the power wheel is driven to rotate, the height position of the color sensor is adjusted by matching the fixing force wheel and the double chains, blood samples of test tubes of different specifications can be detected, and the practicability is high.
Detailed Description
The invention will be further explained and explained with reference to the drawings and the embodiments in the description. The step numbers in the embodiments of the present invention are set for convenience of illustration only, the order between the steps is not limited at all, and the execution order of each step in the embodiments can be adaptively adjusted according to the understanding of those skilled in the art.
Referring to fig. 1, the embodiment of the invention provides a plasma chyle and hemolysis detection device suitable for test tubes of multiple specifications, which comprises a base, wherein a signal processing system, a power supply and a motor are arranged in the base, a plurality of test tube grids are arranged on the base, hard plastic shells are arranged on the test tube grids, color sensors are arranged on the side wall or the rear wall of each hard plastic shell, a fixing force wheel is arranged above each color sensor, a power wheel is arranged below each color sensor, the fixing force wheel and the power wheel are connected through double chains, and the color sensors are positioned between the double chains;
the color sensor is used for acquiring color sensing signals of a blood sample in a test tube placed in the test tube lattice in real time;
the signal processing system is used for generating a detection result of the chyle degree and the hemolysis degree of the plasma according to the blood color sensing signal; triggering a corresponding control signal, and then sending the control signal to the motor;
the motor is used for rotating by a corresponding amplitude according to a control signal of the signal processing system so as to drive the power wheel to rotate by a corresponding number of turns, so that the height position of the color sensor is adjusted;
and the power supply is used for providing working power supply signals for the signal processing system, the motor and the color sensor.
Further as a preferred embodiment, a side wall distance sensor is arranged on one side of the inner side wall of the hard plastic shell;
the side wall distance sensor is used for measuring the distance between the side wall of the inner side of the rigid plastic shell and the side wall of the test tube.
Further as a preferred embodiment, the bottom of the hard plastic shell is provided with a bottom distance sensor;
the bottom distance sensor is used for measuring the distance between the bottom of the inner side of the hard plastic shell and the bottom of the test tube.
Further as a preferred embodiment, a gravity sensor is further arranged on the hard plastic shell;
wherein, the gravity inductor is used for acquiring the gravity signal of the test tube in the test tube lattice in real time.
Based on the device shown in fig. 1, the embodiment of the invention also provides a method for detecting plasma chyle and hemolysis of a multi-specification test tube, which comprises the following steps:
acquiring a color sensing signal of a blood sample in a test tube placed in a test tube lattice in real time through a color sensor;
triggering a corresponding control signal through a signal processing system according to the blood color sensing signal, and sending the control signal to the motor;
the motor rotates by a corresponding amplitude according to a control signal of the signal processing system, and then drives the power wheel to rotate by a corresponding number of turns so as to realize the height position adjustment of the color sensor;
the signal processing system, the motor and the color sensor are provided with working power supply signals through a power supply.
Further as a preferred embodiment, the method further comprises the following steps:
and measuring the distance between the inner side wall of the rigid plastic shell and the side wall of the test tube by using a side wall distance sensor.
Further as a preferred embodiment, the method further comprises the following steps:
and measuring the distance between the bottom of the inner side of the hard plastic shell and the bottom of the test tube by using a bottom distance sensor.
Further as a preferred embodiment, the method further comprises the following steps:
and acquiring the gravity signal of the test tube in the test tube lattice in real time through a gravity sensor.
Further as a preferred embodiment, the method further comprises the following steps:
calculating the mass of the blood according to the gravity of the empty test tube and the gravity of the centrifuged test tube;
calculating the volume of blood based on the mass of blood;
calculating the diameter of the test tube according to the distance between the inner side wall of the rigid plastic shell and the side wall of the test tube and the diameter of the rigid plastic shell;
calculating the height of the blood liquid level in the test tube according to the diameter of the test tube;
calculating the height of the liquid level of the plasma and the height of the midpoint of the plasma according to the height of the liquid level of the blood and the distance between the bottom of the inner side of the hard plastic shell and the bottom of the test tube;
and adjusting the height position of the color sensor according to the plasma liquid level height and the plasma midpoint height.
Further, as a preferred embodiment, the step of adjusting the height position of the color sensor according to the plasma level height and the plasma midpoint height includes the following steps:
acquiring an initial starting position of a color sensor, and calculating the distance between the initial starting position and the bottom of the hard plastic shell;
calculating the moving height of the color sensor according to the distance between the bottom of the inner side of the hard plastic shell and the bottom of the test tube, the height of the blood liquid level and the distance between the initial starting position and the bottom of the hard plastic shell;
calculating the number of turns of the power wheel according to the moving height of the color sensor;
generating a working control signal of the motor through a signal processing system according to the rotating number of turns of the power wheel;
and a signal processing system sends a working control signal to the motor to control the motor to rotate so as to adjust the height position of the color sensor.
The working principle of the detection device for plasma chyle and hemolysis suitable for the multi-specification test tube of the invention is explained in detail below with the accompanying drawings of the specification:
as shown in fig. 2, the color sensor 1.4 is located on the side wall or the rear wall of the rigid plastic shell 1.1;
a fixed power wheel 1.8 and a power wheel 1.9 are arranged above the side wall or the rear wall of the hard plastic shell 1.1;
as shown in figure 3, the fixed force wheel 1.8 is connected with the power wheel 1.9 through double chains, the color sensor 1.4 is positioned between the double chains, and the movement of the double chains can drive the color sensor 1.4 to move up and down on the hard plastic shell 1.1.
A side wall distance sensor 1.6 is additionally arranged on the left side or the right side or the front side and the rear side of the inner side wall of the rigid plastic shell 1.1 and used for measuring the distance between the inner side wall of the rigid plastic shell 1.1 and the side wall of the test tube.
A bottom distance sensor 1.7 is added in the middle of the bottom of the inner side of the rigid plastic shell 1.1 and is used for measuring the distance between the bottom of the inner side of the rigid plastic shell 1.1 and the bottom of the test tube.
Because the same collection test tube but different blood volumes of adopting or use test tube height and/or size when gathering the sample inequality, it is unsettled to lead to plasma in the test tube for the position of test tube check after the centrifugation, therefore color inductor 1.4 need constantly adjust the height in the test tube check, just can effectively discern the colour of plasma in the test tube. The invention can automatically adjust the height of the color sensor 1.4 according to the test tube after being placed in the centrifuge, and has high practicability.
In addition, as shown in fig. 2, the detection device of the present invention comprises a test tube 1, a signal processing system 2, a base 3, a power source 4, a storage 5, and a display 7; test tube lattice 1 is the cuboid structure of adiactinic top open-ended, and blood sample test tube 6 after a centrifugation is placed to each test tube lattice 1, test tube lattice 1 is used for responding to the information of blood sample test tube 6 after the centrifugation and gives signal processing system 2 with information transmission, a plurality of test tube lattices 1 can be settled to base 3, signal processing system 2 is arranged in receiving gravity inductor 1.2, RFID chip reader 1.3 and color inductor 1.4's signal and will handle back information transmission to display 7, power 4 is arranged in providing electric power for signal processing system 2, gravity inductor 1.2, color inductor 1.4, RFID chip reader 1.3, yellow-light 1.5 and display 7.
Preferably, the test tube lattice 1 consists of a hard plastic shell 1.1, a gravity sensor 1.2, a color sensor 1.4, an RFID chip reader 1.3 and a yellow light lamp 1.5, wherein the hard plastic shell 1.1 is a cuboid with an open upper end, and the hard plastic shell 1.1 has a round upper end; the diameter of the opening at the upper end of the hard plastic shell 1.1 is larger than the diameter of the centrifuged blood sample test tube 6 and smaller than the diameter of the centrifuged blood sample test tube 6 cap.
Hard plastic shell 1.1 upper end opening inner wall passes through the 6 wall contacts of blood sample test tube after elastic material and centrifugation, and blood sample test tube 6 after both accessible elastic material fixed centrifugation can avoid outside light source to get into again and influence colour inductor 1.4 response result in the test tube check 1, the optional black silica gel that uses of elastic material, the opening of silica gel slightly is less than the external diameter of test tube.
Gravity inductor 1.2 is located stereoplasm plastic shell 1.1 upper end for whether there is gravity above the response test tube check 1, and send signal processing system 2 with gravity signal. When gravity exists on the test tube lattice 1, the test tube lattice 1 is inserted with the centrifuged blood sample test tube 6, and when no gravity exists above the test tube lattice 1, the test tube lattice 1 is not inserted with the centrifuged blood sample test tube 6.
The RFID chip reader 1.3 is located on the left side of the rigid plastic shell 1.1, and after the test tube lattice 1 is inserted into the centrifuged blood sample test tube 6, the RFID chip reader 1.3 reads information of a built-in RFID chip tag on the centrifuged blood sample test tube 6 and sends the information to the signal processing system 2.
The color sensor 1.4 is located on the inner wall of the front side of the rigid plastic shell 1.1, and the color sensor 1.4 is used for sensing the RGB value of the plasma color in the centrifuged blood sample tube 6 and sending the information to the signal processing system 2.
The yellow light lamp 1.5 is located at the bottom of the inner side of the hard plastic shell 1.1, and the yellow light lamp 1.5 is used for providing a light source for the color sensor 1.4.
The signal processing system 2 is positioned in the base 3, the signal processing system 2 is used for receiving the information of the gravity sensor 1.2, the color sensor 1.4 and the RFID chip reader 1.3 and sending the processed information to the display 7, when the gravity sensor 1.2 senses that a gravity signal exists above the test tube lattice 1, the signal processing system 2 sends a signal to the RFID chip reader 1.3, the RFID chip reader 1.3 reads information of a built-in RFID chip label on the centrifuged blood sample test tube 6 and sends the read information to the signal processing system 2, the signal processing system 2 sends the read information to the display 7, meanwhile, the signal processing system 2 sends signals to the color sensor 1.4 and the yellow light lamp 1.5, the yellow light lamp 1.5 is turned on, the color sensor 1.4 senses the RGB value of the blood plasma and sends the RGB value to the signal processing system 2, the signal processing system 2 obtains the degree of chyle and the degree of hemolysis of the detected plasma and sends the results to a display 7.
The power supply 4 is located in the base 3, and the power supply 4 is used for providing power for the signal processing system 2, the gravity sensor 1.2, the color sensor 1.4, the RFID chip reader 1.3, the yellow light lamp 1.5 and the display 7.
The storage device 5 is located in the base 3 and used for storing test tube information and the chyle degree and the hemolysis degree of plasma in the test tube.
The display 7 is positioned in front of the upper end of the test tube lattice 1 and is used for displaying the information of the centrifuged blood sample test tube 6 sent by the signal processing system 2 and the chyle degree and the hemolysis degree of the plasma.
The following describes in detail the specific implementation steps of the method of the present invention for a device for detecting plasma chyle and hemolysis in a multi-format test tube:
s1, calculating the quality of blood;
firstly, placing an empty test tube for blood collection in the device, and reading the gravity W1 of the empty test tube by a gravity sensor in the device;
then, placing the centrifuged test tube into the device, and reading the gravity W2 of the centrifuged test tube by a gravity sensor;
finally, the two obtained gravity change results Δ W ═ are calculated (W)
2-W
1) Obtaining the quality of the collected blood
Wherein g is 9.8N/KG.
S2, calculating the volume of the blood;
firstly, according to the density formula
Calculating the volume of blood
Since the relative density of blood with respect to water is 1.05-1.06. The average relative density of 1.055 was selected as the blood density in this example. Thus, the volume of blood is calculated as
S3, calculating the diameter of the test tube;
firstly, the diameter d of the rigid plastic shell is measured by a side wall distance sensor
1And the distance L between the rigid plastic shell and the test tube wall
1And the diameter calculation result of the outer side wall of the test tube is d
2=d
1-2×L
1So that the calculation of the inside diameter of the test tube is d
3=d
2-2L
2Wherein L is
2The thickness of the blood sampling test tube is usually 1 mm, and the area of the blood level in the test tube is calculated
S4, calculating the height H of the blood in the test tube;
the height of the collected blood in the test tube is
Because the blood in human body is composed of plasma and blood cells, the volume percentage of the blood cells in the blood is called hematocrit, the hematocrit of a normal adult male is 40% -50%, the hematocrit of a normal adult female is 37% -48%, and the height of the blood cells in a test tube after centrifugation is about equal to that of the blood cells in the test tube after centrifugation
I.e. the height of the plasma is
S5, calculating the height H of the plasma relative to the test tube lattice3And plasma mid-point height H4;
Firstly, the distance H between the bottom of the rigid plastic shell and the bottom of the test tube is measured by a bottom distance sensor
1The distance of the plasma from the bottom of the rigid plastic shell
The calculated plasma midpoint height is:
s6, calculating the moving height of the color sensor;
when the device is just started, the color sensor of this embodiment is disposed at a fixed position on the device, namely, an initial starting position, between the initial starting position and the bottom of the rigid plastic shellAt a distance H5The color sensor of the present invention can obtain the color of plasma at different locations, wherein:
1) when the device needs to start the color sensor to obtain the color of the blood plasma in the whole test tube, the color sensor is controlled to ascend at a distance
Start recording color at the time of and at a rising distance L
3=H
1+H-H
5The recording of the color is stopped, and the color sensing signal obtained by the color sensor in the ascending distance is the color of the blood plasma in the whole test tube.
2) When the device needs to start the color sensor to sense the color of the middle part of the blood plasma, the color sensor is controlled to ascend at a distance
The color is recorded.
In the present invention, the color sensor rises by a distance L3Equal to the rotating perimeter of the power wheel, so that the ascending distance L of the color sensor can be accurately controlled by controlling the rotating angle of the power wheel3。
After the plasma color sensing operation is completed, the color sensor is controlled to return to the initial starting position in the device to wait for the next sensing operation.
The following describes in detail another embodiment of the method of the present invention for detecting plasma chyle and hemolysis in a multi-size test tube.
Since blood in the human body consists of main plasma and blood cells, the mass of the blood cells is greater than that of the plasma, under the action of centrifugal force. The blood cell layer is arranged at the bottom of the test tube, the blood plasma layer is arranged above the blood cell layer, the respective colors of the blood cell layer and the blood plasma layer are uniformly distributed, but the color of the blood cell layer is obviously different from that of the blood plasma layer. Therefore, the color sensor scans and records the RGB values in the test tube from the bottom of the test tube to obtain RGB value 1, RGB value 2, RGB value 3, RGB value 4, RGB value 5, RGB value 6, RGB value 7 and RGB value 8, respectively.
When the RGB value 1 ≠ RGB value 2 ≠ RGB value 3 ≠ RGB value 4 ≠ RGB value 5 ≠ RGB value 6 ≠ RGB value 7 ≠ RGB value 8, then:
the RGB value 1 is the color value of the test tube lattice;
the RGB values 2, 3 and 4 are the color values of the blood cell layer in the test tube lattice;
the RGB value 5, the RGB value 6 and the RGB value 7 are color values of the plasma layer in the test tube lattice;
the RGB value 8 is the color value of the tube lattice after being inserted into the tube.
Therefore, the RGB value 6 is compared with the RGB values pre-stored in the database, and the database feeds back the chyle index and the degree of hemolysis corresponding to the RGB values.
When the RGB value 1 ═ RGB value 2 ═ RGB value 3 ≠ RGB value 4 ≠ RGB value 5 ≠ RGB value 6 ≠ RGB value 7 ≠ RGB value 8, then:
the RGB value 1, the RGB value 2, the RGB value 3 and the RGB value 4 are color values of the blood cell layer in the test tube lattice;
the RGB value 5, the RGB value 6 and the RGB value 7 are color values of the plasma layer in the test tube lattice;
the RGB value 8 is the color value of the tube lattice after being inserted into the tube.
Therefore, the RGB value 6 is compared with RGB in the database, and the database feeds back the chylomicron index and the degree of hemolysis corresponding to the RGB value.
In addition, the invention can also measure the distance H between the bottom of the rigid plastic shell and the bottom of the test tube through the bottom distance sensor1When the device is just started, the color sensor of this embodiment is disposed at a fixed position on the device, i.e., an initial starting position, and the distance between the initial starting position and the bottom of the rigid plastic shell is H5When H is present5>H1While, the color sensor is lowered H5-H1The distance of (d); when H is present5<H1While, the color sensor rises H1-H5The distance of (d); at this time, the color values of the test tube lattices are recorded, and then RGB values 1, 2, 3, 4, 5, 6, 7 and 8 are obtained.
When the RGB value 1 ═ RGB value 2 ═ RGB value 3 ≠ RGB value 4 ≠ RGB value 5 ≠ RGB value 6 ≠ RGB value 7 ≠ RGB value 8, then:
the RGB value 1, the RGB value 2, the RGB value 3 and the RGB value 4 are color values of the blood cell layer in the test tube lattice;
the RGB value 5, the RGB value 6 and the RGB value 7 are color values of the plasma layer in the test tube lattice;
the RGB value 8 is the color value of the tube lattice after being inserted into the tube.
Therefore, the RGB value 6 is compared with RGB in the database, and the database feeds back the chylomicron index and the degree of hemolysis corresponding to the RGB value.
In summary, the invention has the following advantages:
1. the color sensor can accurately distinguish slight color changes, can objectively observe the chyle degree and the hemolysis degree of the plasma, and particularly can avoid the conditions of non-uniform standard and non-uniform judgment result caused by subjective factors of operators when the collected blood sample has chyle blood and hemolysis simultaneously;
2. the whole test tube lattice is sealed and light-proof, natural light cannot enter the test tube lattice, and meanwhile, a light source required by the color sensor is a yellow light lamp, so that the decomposition of medicines or metabolites in blood plasma under the natural light can be effectively prevented, the detection error is avoided, and the quality of a sample is improved;
3. the motor starts to work according to the control signal of the signal processing system, so that the power wheel is driven to rotate, the height position of the color sensor is adjusted by matching the fixing force wheel and the double chains, blood samples of test tubes of different specifications can be detected, and the motor is wide in application range and high in practicability.
4. According to the invention, the distance between the rigid plastic shell and the test tube is sensed in real time through the side wall distance sensor and the bottom distance sensor, the signal processing system triggers corresponding control signals according to the sensed distance signals to control the work of the modules such as the motor and the display, so that collision between the test tube and the rigid plastic shell due to the close distance can be prevented, and the safety is high.
While the preferred embodiments of the present invention have been illustrated and described, it will be understood by those skilled in the art that various changes in form and details may be made therein without departing from the spirit and scope of the invention as defined by the appended claims.