CN110954699A - Micro-fluidic control disc and detection method using same - Google Patents
Micro-fluidic control disc and detection method using same Download PDFInfo
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- CN110954699A CN110954699A CN201911265643.8A CN201911265643A CN110954699A CN 110954699 A CN110954699 A CN 110954699A CN 201911265643 A CN201911265643 A CN 201911265643A CN 110954699 A CN110954699 A CN 110954699A
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Images
Classifications
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- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
- G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
- G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
- G01N33/68—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing involving proteins, peptides or amino acids
-
- B—PERFORMING OPERATIONS; TRANSPORTING
- B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
- B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
- B01L3/00—Containers or dishes for laboratory use, e.g. laboratory glassware; Droppers
- B01L3/50—Containers for the purpose of retaining a material to be analysed, e.g. test tubes
- B01L3/502—Containers for the purpose of retaining a material to be analysed, e.g. test tubes with fluid transport, e.g. in multi-compartment structures
- B01L3/5027—Containers for the purpose of retaining a material to be analysed, e.g. test tubes with fluid transport, e.g. in multi-compartment structures by integrated microfluidic structures, i.e. dimensions of channels and chambers are such that surface tension forces are important, e.g. lab-on-a-chip
- B01L3/502769—Containers for the purpose of retaining a material to be analysed, e.g. test tubes with fluid transport, e.g. in multi-compartment structures by integrated microfluidic structures, i.e. dimensions of channels and chambers are such that surface tension forces are important, e.g. lab-on-a-chip characterised by multiphase flow arrangements
-
- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
- G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
- G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
- G01N33/53—Immunoassay; Biospecific binding assay; Materials therefor
- G01N33/543—Immunoassay; Biospecific binding assay; Materials therefor with an insoluble carrier for immobilising immunochemicals
- G01N33/54313—Immunoassay; Biospecific binding assay; Materials therefor with an insoluble carrier for immobilising immunochemicals the carrier being characterised by its particulate form
- G01N33/54326—Magnetic particles
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- B—PERFORMING OPERATIONS; TRANSPORTING
- B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
- B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
- B01L2200/00—Solutions for specific problems relating to chemical or physical laboratory apparatus
- B01L2200/10—Integrating sample preparation and analysis in single entity, e.g. lab-on-a-chip concept
-
- B—PERFORMING OPERATIONS; TRANSPORTING
- B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
- B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
- B01L2300/00—Additional constructional details
- B01L2300/08—Geometry, shape and general structure
- B01L2300/0803—Disc shape
- B01L2300/0806—Standardised forms, e.g. compact disc [CD] format
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- B—PERFORMING OPERATIONS; TRANSPORTING
- B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
- B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
- B01L2300/00—Additional constructional details
- B01L2300/08—Geometry, shape and general structure
- B01L2300/0861—Configuration of multiple channels and/or chambers in a single devices
Abstract
The invention discloses a micro-fluidic control disc and a detection method using the same, wherein the micro-fluidic control disc is provided with a sample inlet, a waste liquid groove and a plurality of detection units, each detection unit comprises a sample dividing groove, a reagent pre-installing groove and a measurement cell, the sample dividing groove, the reagent pre-installing groove and the measurement cell are sequentially communicated, the sample dividing groove is connected with the sample inlet, the reagent pre-installing groove is used for packaging a pre-freeze-dried reagent for molecular detection or a pre-freeze-dried reagent for immunoassay, the measurement cell is communicated with the waste liquid groove, and a surface tension valve is arranged in a connecting channel between an outlet of the sample dividing groove and the reagent pre-installing groove. The invention provides a micro-fluidic control disc, which comprises a plurality of detection units, wherein each detection unit comprises a pre-installed reagent groove, different pre-installed reagents can be packaged in the pre-installed reagent grooves according to application requirements, a plurality of items can be detected simultaneously, the micro-fluidic control disc with the same structure can meet the requirements of immunoassay and molecular detection simultaneously, and the micro-fluidic control disc is strong in universality and simple in structure.
Description
Technical Field
The invention belongs to the technical field of immune in-vitro diagnosis, and particularly relates to a micro-fluidic disc and a detection method using the same.
Background
The micro-fluidic technology is to utilize the physical and chemical characteristics and scale effect of multi-phase micro-fluid to generate, control, react, analyze and screen micro-droplet multi-phase micro-functional units in a micro-channel. At present, microfluidic technology is widely applied to immunodetection and molecular diagnosis, but different microfluidic control disks are adopted for immunodetection and molecular diagnosis at present, and a microfluidic control disk suitable for immunodetection and molecular diagnosis at the same time is not available.
Disclosure of Invention
The invention aims to overcome the defects of the prior art and provides a micro-fluidic disk and a detection method using the micro-fluidic disk.
In order to achieve the purpose, the invention adopts the following technical scheme:
the utility model provides a micro-fluidic control dish, micro-fluidic control dish is equipped with introduction port, waste liquid groove and a plurality of detecting element, each detecting element is including dividing appearance groove, pre-installation reagent groove and measuring cell, divide the appearance groove pre-installation reagent groove with the measuring cell communicates in order, divide the appearance groove with the introduction port links to each other, wherein, pre-installation reagent groove is used for encapsulating freeze-dried molecular detection in advance and uses reagent or freeze-dried immunoassay in advance and uses reagent, the measuring cell with waste liquid groove intercommunication, divide the export in appearance groove with install the surface tension valve in the interface channel between the pre-installation reagent groove.
Further, the sample dividing grooves of the plurality of detection units are circumferentially and uniformly distributed around the center of the micro-fluidic control disc.
Further, the reagent for detecting molecules comprises a receptor-coupled magnetic bead, a ligand-labeled primer, and a luminescent signal substance-labeled primer; the reagent for immunoassay comprises a receptor-coupled magnetic bead, a ligand-labeled antibody and a luminescent signal substance-labeled antibody, and/or the reagent for immunoassay comprises the receptor-coupled magnetic bead, the ligand-labeled detection antibody and a luminescent signal substance-labeled antigen.
Furthermore, the sample inlet is communicated with the sample separating grooves through a plurality of component sample flow channels, the sample separating flow channels are positioned at the tail ends along the flow direction of the samples and communicated with the waste liquid groove through redundant sample flow channels, and pressure balance valves are installed in the redundant sample flow channels.
Furthermore, the detection unit also comprises a reaction flow channel, the reagent pre-loading groove is connected with the front end of the reaction flow channel, and the rear end of the reaction flow channel is communicated with the measuring cell.
Further, the reaction flow channel is in a serpentine shape, so that the pre-loaded reagent is fully mixed with the sample.
Furthermore, the waste liquid groove is an annular groove arranged on the periphery of the micro-fluidic control disc, and the measuring cell of each detecting unit is communicated with the waste liquid groove through a waste liquid flow channel.
A method for detecting a sample by using the micro-fluidic control disc, which comprises the following steps,
step S1, placing the micro-flow control disc into a detection instrument, injecting a sample from a sample inlet, and distributing the sample to a sample distributing groove of each detection unit;
step S2, controlling the micro-fluidic control disc to rotate at a first rotating speed and a first acceleration, opening the surface tension valve, enabling the sample in the sample distribution groove to enter a pre-loaded reagent groove to soak the pre-loaded reagent, and entering a reaction state;
step S3, controlling the micro-fluidic disc to rotate at a second rotation speed and a second acceleration, and transferring the sample from the reagent pre-loading groove to the measurement cell;
step S4, adding a magnetic field to adsorb magnetic substances in the reaction liquid at the bottom of the measuring cell by a detection instrument, adding cleaning liquid from the sample inlet, allowing the cleaning liquid to enter each detection unit for cleaning, and allowing the cleaning waste liquid to flow into the waste liquid groove;
step S5, controlling the micro-flow control disc to rotate at a third rotating speed and a third acceleration, and spin-drying the residual cleaning liquid in each detection unit;
and step S6, starting a detection module, and detecting through the window of the measuring cell.
Further, the first rotation speed: the second rotating speed is as follows: third rotation speed 15:20:30, of a nitrogen-containing gas; first acceleration: the second acceleration is: the third acceleration is 1:5: 1.
Further, step S1 includes injecting the sample into the sample inlet through the automatic sample injection device, and allowing the excess sample to enter the waste liquid tank through the excess sample flow channel.
The invention provides a micro-fluidic control disc, which comprises a plurality of detection units, wherein each detection unit comprises a pre-installed reagent groove, different pre-installed reagents can be packaged in the pre-installed reagent grooves according to application requirements, so that the micro-fluidic control disc with the same shape can meet the requirements of immunoassay and molecular detection at the same time, and the micro-fluidic control disc is simple in structure.
Drawings
FIG. 1 is a schematic diagram of the overall structure of a micro-fluidic disk according to the present invention;
fig. 2 is a schematic structural diagram of a detection unit in a microfluidic disk according to the present invention.
Detailed Description
An embodiment of a microfluidic disk according to the present invention is further described with reference to fig. 1. A microfluidic control disk of the present invention is not limited to the description of the following embodiments.
As shown in FIG. 1, a micro-fluidic control disk 12 includes a sample inlet 1, a plurality of detecting units, an excess sample channel 4, a pressure balance valve 5, and a waste liquid tank 11. The detection units can have the same structure, and each detection unit comprises a sample dividing groove 3, a surface tension valve 6, a reagent pre-loading groove 7, a reaction flow channel 8, a measurement pool 9 and a waste liquid flow channel 10.
The sample inlet 1 is located in the middle of a certain radius of the micro-fluidic disc 12 and is connected with the front inlet of the sample dividing groove 3 through the sample dividing flow channel 2. The sample flows into the sample dividing flow channel 2 from the sample inlet 1, and is respectively merged into the sample dividing grooves 3 in the plurality of detection units, and the sample is quantitatively divided by means of the sample dividing grooves 3. The transverse size of the sample separating groove 3 is gradually reduced from the front end inlet to the rear end outlet, and the section of the sample separating groove 3 parallel to the disc surface of the micro-fluidic disc 12 is in an isosceles trapezoid shape.
The outlet at the bottom of the sample dividing groove 3 is communicated with a reagent pre-loading groove 7, a surface tension valve 6 is arranged in a connecting channel between the sample dividing groove 3 and the reagent pre-loading groove 7, when the reaction is needed, the surface tension valve 6 is opened, and the sample flows into the reagent pre-loading groove 7 from the sample dividing groove 3.
The reagent pre-loading groove 7 is connected with the front end of the reaction flow channel 8, and the rear end of the reaction flow channel 8 is communicated with the measuring cell 9. The reagent pre-filled groove 7 is used for packaging pre-filled reagents, and a plurality of detection units can package different reagents according to detection requirements. The reaction flow channel 8 is in a snake shape, and a reaction liquid formed by mixing the sample and the reagent pre-loading groove 7 is fully mixed in the flowing process of the reaction flow channel 8, then flows into the measuring cell 9, and is detected by means of a window at the measuring cell 9.
The bottom of measuring cell 9 passes through waste liquid runner 10 and waste liquid groove 11 intercommunication, and waste liquid groove 11 is located the periphery of miniflow control dish 12 and is the annular, and a plurality of detecting element set up around the central circumference of miniflow control dish 12, and each detecting element's measuring cell 9 all links to each other with waste liquid groove 11 to wash into waste liquid groove 11 with the reaction liquid in the measuring cell 9 when washing.
The sample inlet 1 is communicated with the redundant sample flow passage 4 through the multi-component sample flow passage 2 and the sample dividing groove 3, and the redundant sample flow passage 4 is communicated with the waste liquid groove 11 through the pressure balance valve 5. When a sample needs to be introduced into each sample dividing groove 3, the pressure balance valve 5 is opened, and the sample is uniformly distributed to each sample dividing groove 3, and then the redundant sample flows into the waste liquid groove 11 along the redundant sample flow passage 4. The pressure of the pressure balance valve 5 is regulated to ensure that samples are uniformly distributed in each sample distributing groove 3, and the surface tension valve 6 is in a closed state in the sample distributing process. The multi-component sample flow channel 2 is positioned at the inner ring of the micro-flow control disc 12 and is distributed annularly.
Specifically, 7-15 detection units may be mounted on the micro-fluidic disk 12, and the sample distribution groove 3 of each detection unit is closer to the center of the micro-fluidic disk 12 than the pre-filled reagent groove 7.
The micro-fluidic control disk 12 provided by the invention comprises a plurality of detection units, wherein each detection unit comprises a pre-loaded reagent groove 7, different pre-loaded reagents can be packaged in the pre-loaded reagent grooves 7 according to application requirements, for example, when molecular detection is carried out, the whole molecular detection reagent is freeze-dried and packaged in the pre-loaded reagent grooves 7; when the immunoassay is carried out, different freeze-drying modes are adopted according to the difference between a sandwich method and a competition method. Specifically, in the sandwich immunoassay, the reagents can be mixed and then freeze-dried, and can also be respectively freeze-dried and then packaged; when the competitive immunoassay method is adopted, the magnetic beads and one of the antibody or the antigen can be mixed and then freeze-dried, wherein the antibody and the antigen need to be separately freeze-dried, and the two are prevented from reacting in advance. When detection is carried out, corresponding reagents filled in the reagent pre-loading grooves 7 can be detected according to the adopted detection method, so that the requirements of immunoassay and molecular detection can be met simultaneously by means of the microfluidic control disk 12 with the same structure, and the universality is high.
When the micro flow control disk 12 is used for detection:
(1) the micro-flow control disc 12 is put into a detection instrument, the automatic sample adding device and the pressure balance valve 5 are opened, samples are continuously injected from the sample inlet 1, the samples are uniformly distributed into the sample dividing grooves 3 of all the detection units, and redundant samples enter the waste liquid groove 11 through the redundant sample flow passage 4. Wherein, the micro-flow control disc 12 is placed behind the detecting instrument, the pump valve of the automatic sample adding device is opened, and the sample is injected into the sample inlet 1 through the sample adding needle.
(2) The micro-fluidic control disc 12 is controlled to rotate at a first rotating speed and a first acceleration, the surface tension valve 6 is opened, the sample in the sample distribution groove 3 enters the reagent pre-loading groove 7 through a pipeline, the pre-loading reagent is soaked, and the reaction state is achieved. The micro-flow control disc 12 is arranged on a centrifugal heating disc, the centrifugal heating disc is connected with a mechanical transmission mechanism, and the motion of the mechanical transmission mechanism is controlled through a motion controller, so that the rotation speed and the motion acceleration of the micro-flow control disc 12 are controlled.
(3) After the micro-fluidic disc 12 rotates at the first rotating speed and the first acceleration for a certain time, and after the pre-filled reagent is fully soaked in the sample, the micro-fluidic disc 12 is controlled to rotate at the second rotating speed and the second acceleration, and the sample is conveyed to the measuring cell 9 from the pre-filled reagent groove 7 through the reaction flow channel 8;
(4) the detecting instrument adds the magnetic field at the bottom of measuring cell 9 and adsorbs the magnetic substance in the reaction liquid, and automatic application of sample device adds the washing liquid from introduction port 1, closes pressure balance valve 5, and the washing liquid can get into each detecting element and wash under the drive of introduction pressure, and the washing waste liquid gets into waste liquid groove 11 through waste liquid runner 10. The magnetic field is provided by a magnetic attraction ring positioned below the centrifugal heating plate, and the magnetic attraction ring can move and is controlled by a motion controller.
(5) And controlling the micro-fluidic disc 12 to rotate at a third rotating speed and a third acceleration to spin-dry the residual cleaning solution in each detection unit.
(6) And the detection module is started and detects through the window 9 of the measuring cell.
The rotation speed ratio of the micro-fluidic disk 12 in the detection process is as follows: a first rotation speed: the second rotating speed is as follows: the third rotation speed is 15:20: 30; first acceleration: the second acceleration is: the third acceleration is 1:5: 1.
When the molecular detection is carried out, the pre-loaded reagent packaged in the pre-loaded reagent tank 7 comprises receptor coupled magnetic beads, a ligand marker and a luminescent signal substance marker primer; the three can be mixed and freeze-dried together, and the freeze-dried reagent is packaged in the reagent pre-loading groove 7. When the sandwich immunoassay is carried out, the pre-loaded reagent packaged in the pre-loaded reagent groove 7 comprises receptor coupling magnetic beads, ligand labeled antibodies and luminescent signaler labeled antibodies; the three components can be mixed and freeze-dried together, and then are packaged after freeze-drying. When the competitive immunoassay is carried out, the pre-loaded reagent packaged in the pre-loaded reagent tank 7 comprises receptor coupling magnetic beads, a ligand labeling detection antibody and a luminescent signaler labeling antigen freeze-dried product; during the freeze-drying operation, the receptor-coupled magnetic beads can be respectively freeze-dried and then packaged, or the receptor-coupled magnetic beads and one of the other two types of the receptor-coupled magnetic beads can be mixed and then freeze-dried, and it is to be noted that the ligand-labeled detection antibody and the luminescent signal substance-labeled antigen need to be respectively freeze-dried and cannot be mixed together for freeze-drying, so that chemical reaction can be avoided after mixing.
The receptor coupled with the magnetic beads comprises but is not limited to an anti-FITC antibody, goat anti-mouse IgG, protin A or protin G, avidin or streptavidin, the ligand of the marker or antibody comprises but is not limited to FITC or biotin, and the luminescent signal comprises but is not limited to acridinium ester, isoluminol, horseradish peroxidase (HRP), alkaline phosphatase AP, β -galactosidase or nanoenzyme.
Example 1, sandwich immunoassay:
mixing a biotinylated capture antibody, a 2AP enzyme-labeled detection antibody and streptavidin coupled super-cis nano magnetic beads, then carrying out pre-freeze-drying, and then placing a pre-freeze-dried reagent in a pre-reagent tank 7; the multiple detection units in the micro-fluidic disc 12 can be combined in different items according to different uses, for example, different sandwich immunoassay reagents are packaged in the reagent pre-loading groove 7 according to different detection items, and then finished products are prepared and stored at normal temperature. When in use, the package is torn and put into a detection instrument to carry out the subsequent detection process.
Example 2, competitive immunoassay:
mixing acridine ester labeled coupling antigen and anti-FITC antibody coupled super-cis nano magnetic beads, pre-lyophilizing, performing independent pre-lyophilization on the FITC labeled detection antibody, and placing two lyophilized pieces formed by pre-lyophilization in a pre-loaded reagent tank 7; in addition, the FITC-labeled detection antibody and the anti-FITC antibody coupled superparamagnetic nanobead may be mixed and then pre-lyophilized, and the acridinium ester-labeled coupled antigen may be separately pre-lyophilized, which is not particularly limited. The multiple detection units in the micro-fluidic disc 12 can be combined into different items according to different purposes, that is, different types of reagents can be filled in the reagent pre-filled grooves 7 to perform competitive immunoassay of different detection items; then packaging into finished products, and storing and transporting at normal temperature. When in use, the package is torn and put into a detection instrument to carry out the subsequent detection process.
Example 3, molecular detection:
the biotin marker, the acridinium ester marker coupling primer and the streptavidin coupling super-cis nano magnetic bead are mixed and then are subjected to pre-freeze-drying treatment, and then are placed in a pre-reagent tank 7. The detection units in the micro-fluidic disc 12 can be combined in different projects according to different purposes, and then packaged into finished products for normal-temperature storage and transportation. When in use, the package is torn and put into a detection instrument to carry out the subsequent detection process.
The amplification method used in the molecular detection is a constant temperature amplification method, and includes, but is not limited to, common loop-mediated constant temperature amplification method LAMP or strand displacement amplification method SDA, rolling circle amplification RCA, nicking enzyme nucleic acid constant temperature amplification method NEMA and recombinant polymerase amplification method RPA.
The foregoing is a more detailed description of the invention in connection with specific preferred embodiments and it is not intended that the invention be limited to these specific details. For those skilled in the art to which the invention pertains, several simple deductions or substitutions can be made without departing from the spirit of the invention, and all shall be considered as belonging to the protection scope of the invention.
Claims (10)
1. A micro-fluidic disk, comprising: the micro-fluidic control disc is provided with a sample inlet, a waste liquid groove and a plurality of detection units, each detection unit comprises a sample dividing groove, a pre-installed reagent groove and a measurement pool, the sample dividing groove, the pre-installed reagent groove and the measurement pool are communicated in sequence, the sample dividing groove is connected with the sample inlet, the pre-installed reagent groove is used for packaging a pre-freeze-dried reagent for molecular detection or a pre-freeze-dried reagent for immunoassay, the measurement pool is communicated with the waste liquid groove, and a surface tension valve is installed in a connecting channel between an outlet of the sample dividing groove and the pre-installed reagent groove.
2. The micro-fluidic disk of claim 1, wherein: the sample dividing grooves of the plurality of detection units are circumferentially and uniformly distributed around the center of the micro-fluidic control disc.
3. The micro-fluidic disk of claim 1, wherein: the reagent for molecular detection comprises receptor coupled magnetic beads, a ligand labeled primer and a luminescent signal substance labeled primer; the reagent for immunoassay comprises a receptor-coupled magnetic bead, a ligand-labeled antibody and a luminescent signal substance-labeled antibody, and/or the reagent for immunoassay comprises the receptor-coupled magnetic bead, the ligand-labeled detection antibody and a luminescent signal substance-labeled antigen.
4. The micro-fluidic disk of claim 1, wherein: the introduction port is through multicomponent appearance runner and a plurality of divide the appearance groove intercommunication, be located terminally along the flow direction of sample divide the appearance runner through unnecessary sample runner with waste liquid groove intercommunication, install the pressure balance valve in the unnecessary sample runner.
5. The micro-fluidic disk of claim 1, wherein: the detection unit further comprises a reaction flow channel, the reagent pre-loading groove is connected with the front end of the reaction flow channel, and the rear end of the reaction flow channel is communicated with the measurement pool.
6. The micro-fluidic disk of claim 5, wherein: the reaction flow channel is in a serpentine shape, so that the pre-loaded reagent is fully mixed with the sample.
7. The microfluidic control disk of any one of claims 1 to 6, wherein: the waste liquid groove is an annular groove arranged on the periphery of the micro-flow control disc, and the measuring cell of each detection unit is communicated with the waste liquid groove through a waste liquid flow channel.
8. A method of testing a sample using a microfluidic disc according to any one of claims 1 to 7, wherein: comprises the steps of (a) carrying out,
step S1, placing the micro-flow control disc into a detection instrument, injecting a sample from a sample inlet, and distributing the sample to a sample distributing groove of each detection unit;
step S2, controlling the micro-fluidic control disc to rotate at a first rotating speed and a first acceleration, opening the surface tension valve, enabling the sample in the sample distribution groove to enter a pre-loaded reagent groove to soak the pre-loaded reagent, and entering a reaction state;
step S3, controlling the micro-fluidic disc to rotate at a second rotation speed and a second acceleration, and transferring the sample from the reagent pre-loading groove to the measurement cell;
step S4, adding a magnetic field to adsorb magnetic substances in the reaction liquid at the bottom of the measuring cell by a detection instrument, adding cleaning liquid from the sample inlet, allowing the cleaning liquid to enter each detection unit for cleaning, and allowing the cleaning waste liquid to flow into the waste liquid groove;
step S5, controlling the micro-flow control disc to rotate at a third rotating speed and a third acceleration, and spin-drying the residual cleaning liquid in each detection unit;
and step S6, starting a detection module, and detecting through the window of the measuring cell.
9. The method of claim 8, wherein: a first rotation speed: the second rotating speed is as follows: third rotation speed 15:20:30, of a nitrogen-containing gas; first acceleration: the second acceleration is: the third acceleration is 1:5: 1.
10. The method of claim 8, wherein: and the step S1 includes injecting the sample into the sample inlet through the automatic sample injection device, and allowing the redundant sample to enter the waste liquid tank through the redundant sample flow channel.
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