CN110946070A - Breeding and utilization of cabbage type rape dominant genic male sterility yellow seed, flower and leaf restoring line - Google Patents
Breeding and utilization of cabbage type rape dominant genic male sterility yellow seed, flower and leaf restoring line Download PDFInfo
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- 238000009395 breeding Methods 0.000 title claims abstract description 30
- 241000231392 Gymnosiphon Species 0.000 title claims abstract description 29
- 240000007124 Brassica oleracea Species 0.000 title claims abstract description 24
- 235000003899 Brassica oleracea var acephala Nutrition 0.000 title claims abstract description 24
- 235000011301 Brassica oleracea var capitata Nutrition 0.000 title claims abstract description 24
- 235000001169 Brassica oleracea var oleracea Nutrition 0.000 title claims abstract description 24
- 206010021929 Infertility male Diseases 0.000 title claims description 13
- 208000007466 Male Infertility Diseases 0.000 title claims description 13
- 230000001488 breeding effect Effects 0.000 title abstract description 11
- 240000002791 Brassica napus Species 0.000 claims abstract description 41
- 235000011293 Brassica napus Nutrition 0.000 claims abstract description 41
- 238000012216 screening Methods 0.000 claims abstract description 16
- 238000012360 testing method Methods 0.000 claims abstract description 11
- 241000196324 Embryophyta Species 0.000 claims description 28
- 230000017260 vegetative to reproductive phase transition of meristem Effects 0.000 claims description 23
- 238000000034 method Methods 0.000 claims description 21
- 238000009331 sowing Methods 0.000 claims description 13
- 238000010187 selection method Methods 0.000 claims description 5
- 238000009394 selective breeding Methods 0.000 claims 1
- 108700003861 Dominant Genes Proteins 0.000 abstract description 3
- 238000009405 line breeding Methods 0.000 abstract description 2
- 238000011084 recovery Methods 0.000 abstract 1
- 238000004321 preservation Methods 0.000 description 6
- 108090000623 proteins and genes Proteins 0.000 description 6
- 238000009396 hybridization Methods 0.000 description 4
- 230000035558 fertility Effects 0.000 description 3
- 238000012252 genetic analysis Methods 0.000 description 3
- 238000000926 separation method Methods 0.000 description 3
- 108700028369 Alleles Proteins 0.000 description 2
- 244000258825 Scaevola taccada Species 0.000 description 2
- 238000005034 decoration Methods 0.000 description 2
- 230000002068 genetic effect Effects 0.000 description 2
- 208000000509 infertility Diseases 0.000 description 2
- 230000036512 infertility Effects 0.000 description 2
- 208000021267 infertility disease Diseases 0.000 description 2
- 238000004519 manufacturing process Methods 0.000 description 2
- 239000000463 material Substances 0.000 description 2
- 238000012986 modification Methods 0.000 description 2
- 230000004048 modification Effects 0.000 description 2
- 239000002245 particle Substances 0.000 description 2
- 238000011160 research Methods 0.000 description 2
- 235000011331 Brassica Nutrition 0.000 description 1
- 241000219198 Brassica Species 0.000 description 1
- 240000009206 Hemerocallis fulva Species 0.000 description 1
- 241000238631 Hexapoda Species 0.000 description 1
- 230000001276 controlling effect Effects 0.000 description 1
- 230000005764 inhibitory process Effects 0.000 description 1
- 239000003550 marker Substances 0.000 description 1
- 230000008774 maternal effect Effects 0.000 description 1
- 230000001105 regulatory effect Effects 0.000 description 1
- 238000003860 storage Methods 0.000 description 1
- 230000009897 systematic effect Effects 0.000 description 1
- 235000001652 xuan cao Nutrition 0.000 description 1
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- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01H—NEW PLANTS OR NON-TRANSGENIC PROCESSES FOR OBTAINING THEM; PLANT REPRODUCTION BY TISSUE CULTURE TECHNIQUES
- A01H1/00—Processes for modifying genotypes ; Plants characterised by associated natural traits
- A01H1/02—Methods or apparatus for hybridisation; Artificial pollination ; Fertility
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- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01H—NEW PLANTS OR NON-TRANSGENIC PROCESSES FOR OBTAINING THEM; PLANT REPRODUCTION BY TISSUE CULTURE TECHNIQUES
- A01H1/00—Processes for modifying genotypes ; Plants characterised by associated natural traits
- A01H1/04—Processes of selection involving genotypic or phenotypic markers; Methods of using phenotypic markers for selection
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Abstract
The invention provides a breeding method and application of a brassica napus dominant genic male sterile restoring line, belonging to the technical field of brassica napus three-line breeding. The breeding method of the invention uses yellow seed and round leaf selfing lines as female parent and black seed and flower leaf recovery lines as male parent to hybridize, obtaining F1 generation; then through continuous selfing, combining with the oriented breeding of seed coat color and leaf type, obtaining yellow seed and flower leaf selfing line, and then through test cross identification screening out yellow seed and flower leaf restoring line. The character that the floral leaf mutant in the leaf phenotype of the cabbage type rape is controlled by incomplete dominant gene is applied to controlling the purity of the hybrid, namely the hybrid is an intermediate type of parents, namely a half floral leaf, and the false hybrid is represented as a floral leaf or a round leaf; and the oil content of the bred new restorer is obviously improved.
Description
Technical Field
The invention belongs to the technical field of three-line breeding of cabbage type rape, and particularly relates to breeding and utilization of a cabbage type rape obvious genic male sterile yellow seed and flower leaf restoring line.
Background
Systematic genetic analysis is carried out on dominant nuclear male sterile line material-preferably 3A by plum forest and the like of Shanghai city agricultural scientific institute, and the inventor thinks that: preferably, the fertility of 3A and derivative lines thereof is controlled by 2 pairs of dominant genes, Ms is a dominant sterile gene, and Rf is a dominant restorer gene, which can inhibit the expression of the Ms sterile gene and restore fertility. The invention discloses a cabbage type rape nuclear male sterility three-line seed production method established based on the genetic theory, which is invented in the national invention patent ZL 89109310.9. Zhongxi honor et al utilize the patent of the invention to breed a plurality of double-low rape dominant genic male sterile three-line hybrid seeds, wherein 'Huazai No. 7' national censorship oil 2004019 'and' Huazai No. 9 'national censorship oil 2008006' pass through the national variety approval in turn. In addition, the research result of effectively improving the purity of the hybrid seeds, namely the cabbage type rape dominant genic male sterile orange daylily homozygous two-type line and the application thereof, is published in the national invention patent ZL 200610029259.4.
Subsequently, different researchers conducted intensive studies on dominant genic male sterility of brassica napus, such as: song dynasty and others are considered to be controlled by multiple alleles of the same locus through genetic analysis of another suitable 3A derivative line 609AB, namely Ms is a dominant male sterile locus, Mf is an allelic dominant inhibition locus of Ms, Ms is a normal fertile locus, and the recessive relationship is Mf>Ms>ms. The Hongteng analyzes the 3A-adapted derivative line RS1046AB by means of the same genetic analysis method, proves that the 3A-adapted male sterile line is regulated by a multi-allelic genetic pattern, and the like. The subsequent researchers distinguish the sterile site from other male sterile sites in rape and systematically name the sterile site, unify the sterile sitesThe point is named as BnMs5, and the corresponding multiple alleles are BnMs5 respectivelya(restoring genotype, corresponding to Rf), BnMS5b(sterile genotype, corresponding Ms) and BnMs5c(normally fertile or temporary maintainer line genotype corresponding to the original ms), and the molecular basic research proves that the dominant-recessive relation among the 3 allelic genotypes is BnMs5a>BnMs5b>BnMs5c。
However, in the hybrid seeds prepared by the conventional cabbage type rape restorer line, false hybrids are difficult to identify, and the obtained seeds are mostly black seed coats and have low oil content.
Disclosure of Invention
In view of the above, the present invention aims to provide a method for breeding a brassica napus dominant genic male sterile restorer line, wherein yellow seed characters and flower leaf characters are introduced into the brassica napus dominant genic male sterile restorer line, and the restorer line can be used to improve the oil content of the restorer line and hybrid thereof, effectively eradicate false hybrids in the seedling stage, and fully utilize the advantages of the hybrids.
In order to achieve the above object, the present invention provides the following technical solutions:
the invention provides a breeding method of a brassica napus dominant genic male sterile restoring line, which comprises the following steps: (1) crossing with the Brassica napus selfing line with yellow seeds and round leaves as female parent and the Brassica napus restorer line with black seeds and flower leaves as male parent to obtain F1 generation;
(2) selfing the individuals in the F1 generation to obtain an F2 generation;
(3) screening seeds with yellow brown seed coats in F2 generations, independently sowing, and selecting single plants of the flower leaves or the semiflower leaves for selfing in the flowering period to obtain F3 generations;
(4) screening seeds with yellow brown seed coats in F3 generations for separate sowing, and selecting single plants of the flower leaves for selfing in a flowering period to obtain F4 generations;
(5) screening seeds with yellow brown seed coats in F4 generations, independently sowing, and performing individual plant selfing of selective mosaic leaves in a flowering period to obtain a cabbage type rape dominant genic male sterility restoring line; the phenotype of the brassica napus dominant genic male sterile restoring line is yellow seeds and flower leaves.
Preferably, the female parent variety in step (1) comprises 13-3169 and has the genotype of Ms5cMs5c。
Preferably, the variety of the male parent in the step (1) comprises 13-3096, and the genotype is Ms5aMs5a。
Preferably, the screening method in steps (3), (4) and (5) comprises a size selection method.
Preferably, after the dominant genic male sterile restoring line of the cabbage type rape is obtained, the identification of the dominant genic male sterile restoring line of the cabbage type rape is also included.
Preferably, the method of identification comprises the steps of: carrying out test cross by taking sterile plants in the dominant genic male sterile heterozygous two-type line as female parents and taking individuals with the F4 generation intermediate phenotype of brown and yellow seed coat and leaf type as flower leaves as parents; and when the progeny of the test cross are fertile, the male parent of the test cross combination is the cabbage type rape dominant genic male sterile restoring line.
Preferably, the variety of the sterile plant in the dominant genic male sterile heterozygous two-type line comprises 17-3281, and the genotype is Ms5bMs5c。
Preferably, the genotype of the brassica napus dominant genic male sterile restorer line in the step (5) is Ms5aMs5a。
The invention also provides application of the breeding method in improving the oil content of the brassica napus seeds.
The invention provides a breeding method of a cabbage type rape dominant genic male sterile restorer line, which is characterized in that selfing lines of yellow seeds and round leaves are taken as female parents, and restoring lines of black seeds and flower leaves are taken as male parents for hybridization to obtain F1 generations; then through continuous selfing, combining with the directional breeding of seed coat color and leaf type, obtaining yellow seed and flower leaf selfing line, and then through test cross identification screening out the restoring line of yellow seed and flower leaf. In the present invention, the floral leaf mutant in the leaf phenotype of brassica napus is under the control of an incompletely dominant gene, and the round leaf and floral leaf cross F1 represents the intermediate state, hemifloral leaf, of the parent at present. The present invention applies the character to control the purity of hybrid, that is, the hybrid is amphiphilic intermediate type-hemiflower leaf, and the false hybrid is shown as flower leaf or round leaf.
In the embodiment of the invention, the oil content of the new restorer line 18-4243 bred by utilizing the restorer line reaches 48.3 percent, which is obviously higher than 42.6 percent of the original restorer line 13-3096.
Biological preservation information
13-3169 of Brassica napus (Brassica napus L.) in 2019, storing in China center for type culture collection at 29.08.m, wherein the specific address is Wuhan city, Wuchang Lojia mountain, and the storage number is CCTCC No. P201915;
13-3096 Brassica napus (Brassica napus L.) of Brassica, which is preserved in China center for type culture collection at 29.08.7.2019, specifically designated as Wuhan city Lojia mountain, with the preservation number of CCTCC No. P201916;
brassica napus (Brassica napus L.)18-4243, which is preserved in China center for type culture collection at 29.08.2019, specifically, the Wuhan city Lojia mountain, with the preservation number of CCTCC No. P201917.
Drawings
FIG. 1 is a flow chart of the breeding method of the dominant genic male sterility restoring line of Brassica napus of the present invention;
FIG. 2 shows the production pattern of dominant genic male sterile hybrids;
FIG. 3 is a schematic view of the leaf pattern of Brassica napus according to the present invention;
FIG. 4 shows the expression of a pseudo hybrid in a hybrid of the present invention.
Detailed Description
The invention provides a breeding method of a brassica napus dominant genic male sterile restoring line, which comprises the following steps: (1) crossing with the Brassica napus selfing line with yellow seeds and round leaves as female parent and the Brassica napus restorer line with black seeds and flower leaves as male parent to obtain F1 generation;
(2) selfing the individuals in the F1 generation to obtain an F2 generation;
(3) screening seeds with yellow brown seed coats in F2 generations, independently sowing, and selecting single plants of the flower leaves or the semiflower leaves for selfing in the flowering period to obtain F3 generations;
(4) screening seeds with yellow brown seed coats in F3 generations for separate sowing, and selecting single plants of the flower leaves for selfing in a flowering period to obtain F4 generations;
(5) screening seeds with yellow brown seed coats in F4 generations, independently sowing, and performing individual plant selfing of selective mosaic leaves in a flowering period to obtain a cabbage type rape dominant genic male sterility restoring line; the phenotype of the brassica napus dominant genic male sterile restoring line is yellow seeds and flower leaves.
The process of the breeding method of the present invention is preferably as shown in FIG. 1, and the crossing is performed with the cabbage type rape inbred line with yellow seeds and round leaves as female parent and the cabbage type rape restorer line with black seeds and flower leaves as male parent to obtain F1 generation. The female parent variety of the invention preferably comprises 13-3169 and has the genotype of Ms5cMs5c. The 13-3169 strain of the invention is bred by the academy of agricultural sciences of Shanghai city, has the oil content of 46.4 percent, is preserved in the China center for type culture Collection, and has the preservation number of CCTCC No. P201915. The variety of the male parent preferably comprises 13-3096, and the genotype is Ms5aMs5a. The oil content of the 13-3096 bred by the invention is 42.6%, and the oil is preserved in China center for type culture Collection with the preservation number of CCTCC No. P201916. The hybridization method of the present invention is not particularly limited, and a conventional hybridization method in the art may be used.
After the F1 generation is obtained, individuals in the F1 generation are subjected to selfing to obtain an F2 generation. In the F1 generation obtained by the hybridization, the phenotype is the yellow brown of the seed coat, and the leaf type is the half flower leaf. Individuals in the F1 generation are preferably randomly selected for selfing in the invention. The method of selfing is not particularly limited in the present invention, and conventional selfing methods in the art may be used.
After the F2 generation is obtained, seeds with the yellow brown seed coat in the F2 generation are screened and independently sown, and the individual plants of flower leaves or semiflower leaves are selected in the flowering period for selfing to obtain the F3 generation. Phenotypic separation occurs in the F2 generation, preferably, a grain selection method is used for screening, seeds with yellow brown seed coats are screened out for separate sowing, and then single plants of flower leaves or semiflower leaves are selected for selfing in the flowering period. The method of selfing is not particularly limited in the present invention, and conventional selfing methods in the art may be used.
After the F3 generation is obtained, seeds with the yellow brown seed coat in the F3 generation are screened and independently sown, and single plants of flower leaves are selected for selfing in the flowering period to obtain the F4 generation. The invention preferably utilizes a grain selection method for screening, screens out seed with yellow brown seed coat for separate sowing, and then selects a single plant of flower leaves for self-crossing in the flowering period. The method for selfing is not particularly limited in the present invention, and conventional selfing methods in the art can be used.
After the F4 generation is obtained, the seeds with the yellow brown seed coat in the F4 generation are screened and independently sown, and the individual plants of flowers and leaves are selected for selfing in the flowering phase to obtain the brassica napus dominant genic male sterile restoring line; the phenotype of the brassica napus dominant genic male sterile restoring line is yellow seeds and flower leaves. The invention preferably utilizes a grain selection method for screening, screens out seed grains with yellow brown seed coats, independently sows the seed grains, and then selects single plants of flower leaves for selfing in the flowering period. The method for selfing is not particularly limited, and the conventional selfing method in the field can be used. The genotype of the brassica napus dominant genic male sterile restoring line obtained by the invention is Ms5aMs5aThe phenotype is yellow brown of seed coat and leaf of leaf. The obtained brassica napus dominant genic male sterile restoring line is named as 18-4243, and the restoring line is preserved in China center for type culture collection with the preservation number of CCTCCNO.P201917.
After the cabbage type rape dominant nucleic sterility restoring line is obtained, the identification of the cabbage type rape dominant nucleic sterility restoring line is preferably further included. The identification of the invention preferably uses sterile plants in a dominant genic male sterile hybrid two-type line as female parents and uses individuals with phenotypes of F4 generations of yellow-brown seed coats and leaf types as flower leaves as male parents to carry out test cross; and if the progeny of the side crossing shows fertile performance, the brassica napus dominant genic male sterile restoring line is identified to be positive. The variety of the sterile plant in the dominant genic male sterile heterozygous two-type line preferably comprises 17-3281, and the genotype is Ms5bMs5c. The 17-3281 line of the invention is bred by Shanghai city agricultural academy, and the oil content is 43.4%.
The invention also provides application of the breeding method in improving the oil content of the brassica napus seeds. The method for improving the oil content of the brassica napus seeds is preferably the same as the breeding method, and is not repeated herein.
The invention also provides a method for preparing dominant genic male sterile hybrid by using the brassica napus dominant genic male sterile restoring line, and the specific flow is shown in figure 2, wherein in the homozygous two-type line, the genotype is Ms5bMs5bThe homozygous sterile strain and the genotype are Ms5aMs5bThe fertile plants of (a) account for 50% each. Removing fertile plants just before flowering, taking the rest homozygous sterile plants as female parent and taking genotype as Ms5cMs5cPollinating the temporary maintainer line to obtain the genotype Ms5bMs5cThe whole sterile line of (3); then using the complete sterile line as female parent and using its genotype as Ms5aMs5aPollinating the restorer line to obtain hybrid F1 seed.
The invention also provides a method for eradicating the false hybrid in the hybrid at the seedling stage by utilizing the leaf type marker character of the brassica napus dominant genic male sterile restoring line, which is specifically shown in figure 4: the hybrid F1 seed produced with the sterile line as female parent and the floral leaf restoring line as male parent is sowed to obtain semi-floral leaf. Because the exogenous pollen carried by the insect has no mosaic gene and no restoring gene, the sterile line can not be restored to fertility, and the part of false hybrid is shown as garden leaves after sowing and should be eradicated; when the seed field is harvested and degranulated, the floral leaf restoring line may be mixed with the hybrid, and this part of false hybrid is shown as floral leaf after sowing and should be eradicated.
The following will explain in detail the breeding method and application of the dominant genic male sterility restorer line of Brassica napus provided in the present invention with reference to the examples, but they should not be construed as limiting the scope of the present invention.
Example 1
Configuration of F1:
the genotype of the cabbage type rape yellow seed and round leaf inbred line '13-3169' is Ms5cMs5cIs bred by Shanghai city agricultural science institute, and has oil content of 46.4%;
cabbage type rape black seed, flower and leaf restoring line '13-3096', and gene type Ms5aMs5aThe strain is bred by Shanghai city agricultural science institute, and the oil content is 42.6 percent.
In the 3-month rape flowering period in 2014, the yellow seed and round leaf selfing line '13-3169' is used as a female parent, and the black seed and flower leaf restoring line '13-3096' is used as a male parent to obtain F1 generation seeds in a hybrid mode, and the F1 generation seeds are named as 14-4006. F1 seeds were yellow brown due to maternal effect.
Example 2
Breeding of dominant genic male sterile yellow seed restoring line' 17-4305-1
1. Pedigree breeding
The F1 population '14-4006' was sown in 2014 10 months, and the leaf type at the seedling stage appeared as a parental intermediate type-semiflower leaf (as shown in FIG. 3). And (3) selecting strains from an F1 population at the flowering period of the rape of 3 months in 2015, bagging and selfing. The seed coat of the F2 generation seed after selfing has black and yellow separation.
The F2 population '15-4019' has separated leaf types, including three types, namely floral leaf, hemifloral leaf and round leaf. In the flowering period of the rape of 3 months in 2016, selecting a half-flower leaf from an F2 population, and bagging and selfing the single plant.
The seed coat of the F3 generation seed still has black and yellow separation. Yellow seeds with lighter seed coat color are selected from the seeds of the group as materials for continuous breeding.
Yellow seed progeny '16-4407' obtained by single-particle selection in the F3 population still have separated leaf types. In the flowering period of rape of 3 months in 2017, selecting flowers and leaves from the group, and bagging for selfing. Seeds of generation F4 were yellow or brownish yellow. Yellow seeds with lighter seed coat color are selected from the seeds of the group as materials for continuous breeding.
Yellow seed progeny '17-4305' obtained from F4 generation population by single-particle selection, and the leaf type is mosaic. In the flowering period of rape 3 months in 2018, single plants are bagged for selfing to obtain F5 generation seeds '17-4305-1' of yellow seeds, the oil content of which is 48.3 percent and is obviously higher than that of a restorer line '13-3096'.
2. Identification of restorer lines
In the flowering period of rape 3 months in 2018, a test cross combination is prepared by taking a sterile plant in a dominant genic male sterile heterozygous two-type line '17-3281' as a female parent and taking a single plant '17-4305-1' in a F4 generation population '17-4305' as a male parent. In the flowering period of rape 3 months in 2019, 18 strains are counted in the test cross combination of '18-3321', and all the strains are fertile strains. This result showed that the genotype of the individual strain ` 17-4305-1 ` was Ms5aMs5aThe self-bred progeny 18-4243' is the yellow seed and flower leaf restoring line.
The present invention provides a method for breeding cabbage type rape dominant genic male sterility restoring line, and is characterized by that the leaf type character can be used for controlling purity of hybrid seed, i.e. the hybrid seed is made into the intermediate type of two parents-hemifloral leaf, and the false hybrid seed is made into floral leaf or round leaf, and the false hybrid seed can be removed by means of seedling stage, so that it can raise purity of hybrid seed, fully utilize heterosis, and the restored restoring line can obviously raise oil content of seed.
The foregoing is only a preferred embodiment of the present invention, and it should be noted that, for those skilled in the art, various modifications and decorations can be made without departing from the principle of the present invention, and these modifications and decorations should also be regarded as the protection scope of the present invention.
Claims (9)
1. A breeding method of a cabbage type rape dominant genic male sterility restoring line comprises the following steps: (1) crossing with the Brassica napus selfing line with yellow seeds and round leaves as female parent and the Brassica napus restorer line with black seeds and flower leaves as male parent to obtain F1 generation;
(2) selfing the individuals of the F1 generation to obtain an F2 generation;
(3) screening seeds with yellow brown seed coats in F2 generations, independently sowing, and selecting single plants of the flower leaves or the semiflower leaves for selfing in the flowering period to obtain F3 generations;
(4) screening seeds with yellow brown seed coats in F3 generations for separate sowing, and selecting single plants of the flower leaves for selfing in a flowering period to obtain F4 generations;
(5) screening seeds with yellow brown seed coats in F4 generations, independently sowing, and performing individual plant selfing of selective mosaic leaves in a flowering period to obtain a cabbage type rape dominant genic male sterility restoring line; the phenotype of the brassica napus dominant genic male sterile restoring line is yellow seeds and flower leaves.
2. The breeding method according to claim 1, wherein the female parent in the step (1) comprises 13-3169 and the female parent has the genotype of Ms5cMs5c。
3. The breeding method according to claim 1, wherein the variety of the male parent in the step (1) comprises 13-3096 and has the genotype of Ms5aMs5a。
4. The selective breeding method according to claim 1, wherein the screening in steps (3), (4) and (5) comprises a size selection method.
5. The breeding method of claim 1, further comprising identifying the restorer line for dominant genic male sterility of Brassica napus after obtaining the restorer line for dominant genic male sterility of Brassica napus.
6. The breeding method according to claim 5, wherein the identification method comprises the following steps: carrying out test cross by taking sterile plants in the dominant genic male sterile heterozygous two-type line as female parents and taking individuals with the F4 generation intermediate phenotype of brown and yellow seed coats and leaf type as flower and leaves as male parents; and when the progeny of the test cross are fertile, the male parent of the test cross combination is the cabbage type rape dominant genic male sterile restoring line.
7. The breeding method of claim 6, wherein the variety of the sterile plant in the dominant genic male sterile heterozygous two-type line comprises 17-3281 and the genotype is Ms5bMs5c。
8. The breeding method according to claim 1, characterized by the steps ofThe genotype of the brassica napus dominant genic male sterile restoring line in the step (5) is Ms5aMs5a。
9. Application of the breeding method of any one of claims 1 to 8 in improving the oil content of brassica napus seeds.
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Citations (4)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN1108874A (en) * | 1994-12-28 | 1995-09-27 | 陕西省农垦科研中心 | Selective breeding technique for Brassica type hybrid rape |
| CN102172214A (en) * | 2011-03-07 | 2011-09-07 | 江苏省农业科学院 | Method for breeding rape floral leaf cytoplasmic male-sterility restoring line |
| CN105638447A (en) * | 2016-03-29 | 2016-06-08 | 湖北利众种业科技有限公司 | Breeding method for increasing rape nuclear sterile line yield and improving seed pureness |
| US20190254247A1 (en) * | 2016-06-23 | 2019-08-22 | Chengdu Academy of Agricultural and Forestry Sciences | Method for breeding brassica napus varieties and materials with double haploid induction line of rapeseed |
-
2019
- 2019-09-02 CN CN201910823877.3A patent/CN110946070A/en active Pending
Patent Citations (4)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN1108874A (en) * | 1994-12-28 | 1995-09-27 | 陕西省农垦科研中心 | Selective breeding technique for Brassica type hybrid rape |
| CN102172214A (en) * | 2011-03-07 | 2011-09-07 | 江苏省农业科学院 | Method for breeding rape floral leaf cytoplasmic male-sterility restoring line |
| CN105638447A (en) * | 2016-03-29 | 2016-06-08 | 湖北利众种业科技有限公司 | Breeding method for increasing rape nuclear sterile line yield and improving seed pureness |
| US20190254247A1 (en) * | 2016-06-23 | 2019-08-22 | Chengdu Academy of Agricultural and Forestry Sciences | Method for breeding brassica napus varieties and materials with double haploid induction line of rapeseed |
Non-Patent Citations (4)
| Title |
|---|
| 刘志文等: "甘蓝型油菜种皮颜色的遗传分析", 《华北农学报》 * |
| 张瑞茂等: "甘蓝型双低杂交油菜新品种金农油1号的选育", 《贵州农业科学》 * |
| 张瑞茂等: "甘蓝型高产高油分双低杂交油菜新品种油研50的选育", 《种子》 * |
| 陈社员等: "高含油量黄籽杂交油菜品种湘杂油631的选育", 《作物研究》 * |
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