CN110926911A - Pinus massoniana dyeing kit and dyeing method thereof - Google Patents
Pinus massoniana dyeing kit and dyeing method thereof Download PDFInfo
- Publication number
- CN110926911A CN110926911A CN201911335046.8A CN201911335046A CN110926911A CN 110926911 A CN110926911 A CN 110926911A CN 201911335046 A CN201911335046 A CN 201911335046A CN 110926911 A CN110926911 A CN 110926911A
- Authority
- CN
- China
- Prior art keywords
- solution
- glacial acetic
- acetic acid
- aqueous solution
- acid aqueous
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Pending
Links
- 238000004043 dyeing Methods 0.000 title claims abstract description 25
- 238000000034 method Methods 0.000 title claims abstract description 20
- 241000018650 Pinus massoniana Species 0.000 title claims abstract description 12
- 235000011609 Pinus massoniana Nutrition 0.000 title claims abstract description 11
- QTBSBXVTEAMEQO-UHFFFAOYSA-N Acetic acid Chemical compound CC(O)=O QTBSBXVTEAMEQO-UHFFFAOYSA-N 0.000 claims abstract description 76
- 239000000243 solution Substances 0.000 claims abstract description 63
- 239000007864 aqueous solution Substances 0.000 claims abstract description 40
- 229960000583 acetic acid Drugs 0.000 claims abstract description 38
- 239000012362 glacial acetic acid Substances 0.000 claims abstract description 38
- XOSXWYQMOYSSKB-LDKJGXKFSA-L water blue Chemical compound CC1=CC(/C(\C(C=C2)=CC=C2NC(C=C2)=CC=C2S([O-])(=O)=O)=C(\C=C2)/C=C/C\2=N\C(C=C2)=CC=C2S([O-])(=O)=O)=CC(S(O)(=O)=O)=C1N.[Na+].[Na+] XOSXWYQMOYSSKB-LDKJGXKFSA-L 0.000 claims abstract description 24
- DHRLEVQXOMLTIM-UHFFFAOYSA-N phosphoric acid;trioxomolybdenum Chemical compound O=[Mo](=O)=O.O=[Mo](=O)=O.O=[Mo](=O)=O.O=[Mo](=O)=O.O=[Mo](=O)=O.O=[Mo](=O)=O.O=[Mo](=O)=O.O=[Mo](=O)=O.O=[Mo](=O)=O.O=[Mo](=O)=O.O=[Mo](=O)=O.O=[Mo](=O)=O.OP(O)(O)=O DHRLEVQXOMLTIM-UHFFFAOYSA-N 0.000 claims abstract description 23
- RZUBARUFLYGOGC-MTHOTQAESA-L acid fuchsin Chemical compound [Na+].[Na+].[O-]S(=O)(=O)C1=C(N)C(C)=CC(C(=C\2C=C(C(=[NH2+])C=C/2)S([O-])(=O)=O)\C=2C=C(C(N)=CC=2)S([O-])(=O)=O)=C1 RZUBARUFLYGOGC-MTHOTQAESA-L 0.000 claims abstract description 22
- XLYOFNOQVPJJNP-UHFFFAOYSA-N water Chemical compound O XLYOFNOQVPJJNP-UHFFFAOYSA-N 0.000 claims abstract description 22
- 239000000975 dye Substances 0.000 claims abstract description 21
- 239000012153 distilled water Substances 0.000 claims abstract description 19
- 238000002360 preparation method Methods 0.000 claims abstract description 19
- WZUVPPKBWHMQCE-UHFFFAOYSA-N Haematoxylin Chemical compound C12=CC(O)=C(O)C=C2CC2(O)C1C1=CC=C(O)C(O)=C1OC2 WZUVPPKBWHMQCE-UHFFFAOYSA-N 0.000 claims abstract description 18
- 239000001045 blue dye Substances 0.000 claims abstract description 17
- 239000012192 staining solution Substances 0.000 claims abstract description 10
- 238000010186 staining Methods 0.000 claims abstract description 9
- 239000012188 paraffin wax Substances 0.000 claims description 38
- LFQSCWFLJHTTHZ-UHFFFAOYSA-N Ethanol Chemical compound CCO LFQSCWFLJHTTHZ-UHFFFAOYSA-N 0.000 claims description 18
- 238000005406 washing Methods 0.000 claims description 7
- FGUUSXIOTUKUDN-IBGZPJMESA-N C1(=CC=CC=C1)N1C2=C(NC([C@H](C1)NC=1OC(=NN=1)C1=CC=CC=C1)=O)C=CC=C2 Chemical compound C1(=CC=CC=C1)N1C2=C(NC([C@H](C1)NC=1OC(=NN=1)C1=CC=CC=C1)=O)C=CC=C2 FGUUSXIOTUKUDN-IBGZPJMESA-N 0.000 claims description 6
- GNFTZDOKVXKIBK-UHFFFAOYSA-N 3-(2-methoxyethoxy)benzohydrazide Chemical compound COCCOC1=CC=CC(C(=O)NN)=C1 GNFTZDOKVXKIBK-UHFFFAOYSA-N 0.000 claims description 5
- 230000004069 differentiation Effects 0.000 claims description 3
- 238000007789 sealing Methods 0.000 claims description 3
- 239000008399 tap water Substances 0.000 claims description 3
- 235000020679 tap water Nutrition 0.000 claims description 3
- WZRZTHMJPHPAMU-UHFFFAOYSA-L disodium;(3e)-3-[(4-amino-3-sulfonatophenyl)-(4-amino-3-sulfophenyl)methylidene]-6-imino-5-methylcyclohexa-1,4-diene-1-sulfonate Chemical compound [Na+].[Na+].C1=C(S([O-])(=O)=O)C(=N)C(C)=CC1=C(C=1C=C(C(N)=CC=1)S([O-])(=O)=O)C1=CC=C(N)C(S(O)(=O)=O)=C1 WZRZTHMJPHPAMU-UHFFFAOYSA-L 0.000 claims 1
- 230000000694 effects Effects 0.000 abstract description 5
- 238000007447 staining method Methods 0.000 abstract description 3
- 235000011610 Pinus tabuliformis Nutrition 0.000 abstract 1
- 239000003153 chemical reaction reagent Substances 0.000 abstract 1
- 210000002808 connective tissue Anatomy 0.000 description 7
- 239000000835 fiber Substances 0.000 description 6
- 210000001519 tissue Anatomy 0.000 description 4
- 102000008186 Collagen Human genes 0.000 description 3
- 108010035532 Collagen Proteins 0.000 description 3
- 229920001436 collagen Polymers 0.000 description 3
- 210000004027 cell Anatomy 0.000 description 2
- 230000018044 dehydration Effects 0.000 description 2
- 238000006297 dehydration reaction Methods 0.000 description 2
- 210000004177 elastic tissue Anatomy 0.000 description 2
- 239000007788 liquid Substances 0.000 description 2
- YYYARFHFWYKNLF-UHFFFAOYSA-N 4-[(2,4-dimethylphenyl)diazenyl]-3-hydroxynaphthalene-2,7-disulfonic acid Chemical compound CC1=CC(C)=CC=C1N=NC1=C(O)C(S(O)(=O)=O)=CC2=CC(S(O)(=O)=O)=CC=C12 YYYARFHFWYKNLF-UHFFFAOYSA-N 0.000 description 1
- 241001465754 Metazoa Species 0.000 description 1
- 108010081750 Reticulin Proteins 0.000 description 1
- 210000000577 adipose tissue Anatomy 0.000 description 1
- 230000009286 beneficial effect Effects 0.000 description 1
- 210000004369 blood Anatomy 0.000 description 1
- 239000008280 blood Substances 0.000 description 1
- 210000000988 bone and bone Anatomy 0.000 description 1
- 210000000845 cartilage Anatomy 0.000 description 1
- 230000001413 cellular effect Effects 0.000 description 1
- 229920002678 cellulose Polymers 0.000 description 1
- 239000001913 cellulose Substances 0.000 description 1
- 230000007547 defect Effects 0.000 description 1
- 238000002845 discoloration Methods 0.000 description 1
- 210000003743 erythrocyte Anatomy 0.000 description 1
- 239000012530 fluid Substances 0.000 description 1
- 210000002751 lymph Anatomy 0.000 description 1
- 239000000463 material Substances 0.000 description 1
- 239000011159 matrix material Substances 0.000 description 1
- 239000000203 mixture Substances 0.000 description 1
- 210000001087 myotubule Anatomy 0.000 description 1
- 238000010827 pathological analysis Methods 0.000 description 1
- 231100000915 pathological change Toxicity 0.000 description 1
- 230000036285 pathological change Effects 0.000 description 1
- 239000000126 substance Substances 0.000 description 1
Classifications
-
- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N1/00—Sampling; Preparing specimens for investigation
- G01N1/28—Preparing specimens for investigation including physical details of (bio-)chemical methods covered elsewhere, e.g. G01N33/50, C12Q
- G01N1/30—Staining; Impregnating ; Fixation; Dehydration; Multistep processes for preparing samples of tissue, cell or nucleic acid material and the like for analysis
- G01N1/31—Apparatus therefor
Landscapes
- Health & Medical Sciences (AREA)
- Life Sciences & Earth Sciences (AREA)
- Engineering & Computer Science (AREA)
- Biomedical Technology (AREA)
- Molecular Biology (AREA)
- Physics & Mathematics (AREA)
- Chemical & Material Sciences (AREA)
- Analytical Chemistry (AREA)
- Biochemistry (AREA)
- General Health & Medical Sciences (AREA)
- General Physics & Mathematics (AREA)
- Immunology (AREA)
- Pathology (AREA)
- Investigating Or Analysing Biological Materials (AREA)
Abstract
The invention discloses a Chinese pine staining kit and a staining method thereof. The masson pine staining kit comprises hematoxylin staining solution, ponceau acid fuchsin staining solution, aniline blue staining solution, glacial acetic acid aqueous solution and phosphomolybdic acid aqueous solution. The preparation method of the ponceau acid fuchsin dye solution comprises the following steps: dissolving 4g of ponceau and 2g of acid fuchsin in 495ml of distilled water to obtain a solution A, and adding 5ml of glacial acetic acid into the solution A to obtain ponceau acid fuchsin dye solution; the preparation method of the aniline blue dye solution comprises the following steps: dissolving 10g of aniline blue in 490ml of distilled water to obtain a solution B, and adding 10ml of glacial acetic acid into the solution B to obtain aniline blue dye solution; the preparation method of the glacial acetic acid aqueous solution comprises the following steps: dissolving 10ml of glacial acetic acid in 490ml of distilled water to obtain a glacial acetic acid aqueous solution; the preparation method of the phosphomolybdic acid aqueous solution comprises the following steps: 5g of phosphomolybdic acid was dissolved in 500ml of distilled water to obtain an aqueous phosphomolybdic acid solution. The scheme of the invention has the advantages of low reagent cost, simple use method, good dyeing effect and the like.
Description
Technical Field
The invention relates to the technical field of biology, in particular to a masson pine staining kit and a staining method thereof.
Background
Connective tissue (connective tissue) is composed of cells and a large number of intercellular substances, including stroma, filamentous fibers, and constantly circulating renewed tissue fluid, and has important functional significance.
Connective tissues are widely distributed in animals, and are diverse, including intrinsic connective tissues (loose connective tissues, dense connective tissues, reticular tissues, adipose tissues), blood, lymph, cartilage and bone tissues, all of which share common characteristics: they all originate from the embryonic connective tissue, mesenchymal. In their composition there are, besides cells, a large amount of non-cellular material (amorphous matrix and fibers).
And the fibers include collagen fibers, elastic fibers and reticular fibers. In the conventional pathological analysis techniques, tissue sections are usually stained by a special staining method such as Masson (Masson staining) in order to clarify pathological changes of collagen fibers. Therefore, the development of a masson pine dyeing solution and a dyeing method which have low cost, simple preparation and use methods and good dyeing effect is a problem which is continuously addressed by the technical personnel in the field.
Disclosure of Invention
In order to overcome the defects in the prior art, the embodiment of the invention provides a masson dyeing kit and a dyeing method thereof, which are used for providing the masson dyeing kit with simple use method and good dyeing effect.
The embodiment of the application discloses: a kit for dyeing Pinus massoniana comprises a hematoxylin dye solution, a ponceau acid fuchsin dye solution, an aniline blue dye solution, a glacial acetic acid aqueous solution and a phosphomolybdic acid aqueous solution, wherein:
the preparation method of the ponceau acid fuchsin dye solution comprises the following steps: dissolving 4g of ponceau and 2g of acid fuchsin in 495ml of distilled water to obtain a solution A, and adding 5ml of glacial acetic acid into the solution A to obtain ponceau acid fuchsin dye solution;
the preparation method of the aniline blue dye solution comprises the following steps: dissolving 10g of aniline blue in 490ml of distilled water to obtain a solution B, and adding 10ml of glacial acetic acid into the solution B to obtain aniline blue dye solution;
the preparation method of the glacial acetic acid aqueous solution comprises the following steps: dissolving 10ml of glacial acetic acid in 490ml of distilled water to obtain a glacial acetic acid aqueous solution;
the preparation method of the phosphomolybdic acid aqueous solution comprises the following steps: 5g of phosphomolybdic acid was dissolved in 500ml of distilled water to obtain an aqueous phosphomolybdic acid solution.
Specifically, the kit comprises 15ml of each of ponceau red acid fuchsin dye solution, aniline blue dye solution, glacial acetic acid aqueous solution and phosphomolybdic acid aqueous solution.
Specifically, in the kit, the hematoxylin staining solution, the ponceau acid fuchsin staining solution, the aniline blue staining solution, the glacial acetic acid aqueous solution and the phosphomolybdic acid aqueous solution are respectively packaged by drop bottles.
The embodiment also discloses a dyeing method of the masson pine dyeing kit, which comprises the following steps:
a. dewaxing the paraffin section to water, and then immersing the paraffin section into the hematoxylin staining solution for 2 min;
b. immersing the paraffin section obtained in the step a into the ponceau acid fuchsin dye solution for 1 min;
c. b, immersing the paraffin sections obtained in the step b into the glacial acetic acid aqueous solution for 0.5-1 min, and then washing with distilled water;
d. c, immersing the paraffin section obtained in the step c into the phosphomolybdic acid aqueous solution for differentiation for 3-8 min;
e. d, immersing the paraffin section obtained in the step d into the aniline blue dye solution for 30 s;
f. washing the paraffin sections obtained in the step e with the glacial acetic acid aqueous solution for 30 s;
g. and f, dehydrating the paraffin sections obtained in the step f by using 95% alcohol and absolute alcohol in sequence, and then sealing the paraffin sections.
Specifically, the color of each step in the method is controlled by microscopic examination so as to prevent the glacial acetic acid aqueous solution from being washed excessively and completely discolored.
Specifically, step g further includes: and f, dehydrating the paraffin sections obtained in the step f in 95% alcohol for 30s, and dehydrating in absolute alcohol for 1 min.
Specifically, the method further comprises the step h of: and g, immersing the paraffin sections obtained in the step g in tap water for 2h for decoloring, and then re-dyeing the decolored paraffin sections according to the steps a-g.
The invention has at least the following beneficial effects:
1. in the masson pine staining kit in the embodiment, the preparation method of each staining solution or solution is simple and the cost is low.
2. In the masson pine dyeing kit in this embodiment, the dosage of each dye liquor or solution is equipped rationally, and convenient to use avoids extravagant.
3. The dyeing method provided by the embodiment has the advantages that the operation is simple and easy, and the color can be accurately controlled through microscopic examination, so that the glacial acetic acid aqueous solution is prevented from being washed excessively and completely decolorized, and the dyeing effect is ensured.
In order to make the aforementioned and other objects, features and advantages of the invention more comprehensible, preferred embodiments accompanied with figures are described in detail below.
Detailed Description
The technical solutions in the embodiments of the present invention will be clearly and completely described below, and it is obvious that the described embodiments are only a part of the embodiments of the present invention, and not all embodiments. All other embodiments, which can be derived by a person skilled in the art from the embodiments given herein without making any creative effort, shall fall within the protection scope of the present invention.
The kit for dyeing the masson pine in the embodiment comprises hematoxylin dye solution, ponceau acid fuchsin dye solution, aniline blue dye solution, glacial acetic acid aqueous solution and phosphomolybdic acid aqueous solution, preferably, the liquids of all the components are packaged by adopting one dropping bottle respectively, and each dropping bottle is filled with 15ml of liquid. Wherein, the preparation method of each dye solution or solution is as follows:
the preparation method of the ponceau acid fuchsin dye solution comprises the following steps: dissolving 4g of ponceau and 2g of acid fuchsin in 495ml of distilled water to obtain a solution A, and adding 5ml of glacial acetic acid into the solution A to obtain ponceau acid fuchsin dye solution;
the preparation method of the aniline blue dye solution comprises the following steps: dissolving 10g of aniline blue in 490ml of distilled water to obtain a solution B, and adding 10ml of glacial acetic acid into the solution B to obtain aniline blue dye solution;
the preparation method of the glacial acetic acid aqueous solution comprises the following steps: dissolving 10ml of glacial acetic acid in 490ml of distilled water to obtain a glacial acetic acid aqueous solution;
the preparation method of the phosphomolybdic acid aqueous solution comprises the following steps: 5g of phosphomolybdic acid was dissolved in 500ml of distilled water to obtain an aqueous phosphomolybdic acid solution.
The method for staining the tissue section by adopting the masson staining kit in the embodiment comprises the following steps:
a. dewaxing the paraffin section to water, and then immersing the paraffin section into the hematoxylin staining solution for 2 min;
b. immersing the paraffin section obtained in the step a into the ponceau acid fuchsin dye solution for 1 min;
c. b, immersing the paraffin sections obtained in the step b into the glacial acetic acid aqueous solution for 0.5-1 min, and then washing with distilled water;
d. c, immersing the paraffin section obtained in the step c into the phosphomolybdic acid aqueous solution for differentiation for 3-8 min;
e. d, immersing the paraffin section obtained in the step d into the aniline blue dye solution for 30 s;
f. washing the paraffin sections obtained in the step e with the glacial acetic acid aqueous solution for 30 s;
g. and f, dehydrating the paraffin sections obtained in the step f by using 95% alcohol and absolute alcohol in sequence, and then sealing the paraffin sections.
Preferably, in order to avoid complete discoloration of the paraffin sections due to excessive washing with the glacial acetic acid aqueous solution, color control is performed by microscopic examination as much as possible in each step.
Further, since aniline blue dye solution is easily discolored during the dehydration of alcohol, rapid dehydration is required. Preferably, the paraffin sections obtained in step f are dehydrated in 95% alcohol for 30s and in anhydrous alcohol for 1 min.
Further, if the dyeing effect is not satisfactory, the paraffin section obtained in the step g can be soaked in tap water for 2 hours for decoloring, and then the decolored paraffin section is dyed again according to the steps a to g.
The collagen fibers of the paraffin sections dyed by the masson dyeing kit and the dyeing method thereof in the embodiment are blue; the elastic fiber is brown; myofibers, cellulose, red blood cells are red; the nuclei appear dark blue.
The principle and the implementation mode of the invention are explained by applying specific embodiments in the invention, and the description of the embodiments is only used for helping to understand the method and the core idea of the invention; meanwhile, for a person skilled in the art, according to the idea of the present invention, there may be variations in the specific embodiments and the application scope, and in summary, the content of the present specification should not be construed as a limitation to the present invention.
Claims (7)
1. The kit for dyeing the masson pine is characterized by comprising a hematoxylin dye solution, a ponceau acid fuchsin dye solution, an aniline blue dye solution, a glacial acetic acid aqueous solution and a phosphomolybdic acid aqueous solution, wherein:
the preparation method of the ponceau acid fuchsin dye solution comprises the following steps: dissolving 4g of ponceau and 2g of acid fuchsin in 495ml of distilled water to obtain a solution A, and adding 5ml of glacial acetic acid into the solution A to obtain ponceau acid fuchsin dye solution;
the preparation method of the aniline blue dye solution comprises the following steps: dissolving 10g of aniline blue in 490ml of distilled water to obtain a solution B, and adding 10ml of glacial acetic acid into the solution B to obtain aniline blue dye solution;
the preparation method of the glacial acetic acid aqueous solution comprises the following steps: dissolving 10ml of glacial acetic acid in 490ml of distilled water to obtain a glacial acetic acid aqueous solution;
the preparation method of the phosphomolybdic acid aqueous solution comprises the following steps: 5g of phosphomolybdic acid was dissolved in 500ml of distilled water to obtain an aqueous phosphomolybdic acid solution.
2. The kit of claim 1, wherein the kit comprises 15ml each of fuchsin acid fuchsin dye solution, aniline blue dye solution, glacial acetic acid aqueous solution and phosphomolybdic acid aqueous solution.
3. The kit of claim 2, wherein the hematoxylin stain, ponceau acid fuchsin stain, aniline blue stain, glacial acetic acid aqueous solution, and phosphomolybdic acid aqueous solution are packaged in drop bottles, respectively.
4. A method of staining with the kit for staining with a masson according to any one of claims 1 to 3, comprising the steps of:
a. dewaxing the paraffin section to water, and then immersing the paraffin section into the hematoxylin staining solution for 2 min;
b. immersing the paraffin section obtained in the step a into the ponceau acid fuchsin dye solution for 1 min;
c. b, immersing the paraffin sections obtained in the step b into the glacial acetic acid aqueous solution for 0.5-1 min, and then washing with distilled water;
d. c, immersing the paraffin section obtained in the step c into the phosphomolybdic acid aqueous solution for differentiation for 3-8 min;
e. d, immersing the paraffin section obtained in the step d into the aniline blue dye solution for 30 s;
f. washing the paraffin sections obtained in the step e with the glacial acetic acid aqueous solution for 30 s;
g. and f, dehydrating the paraffin sections obtained in the step f by using 95% alcohol and absolute alcohol in sequence, and then sealing the paraffin sections.
5. The method as claimed in claim 4, wherein the color is controlled by microscopic examination at each step of the method so as to prevent the aqueous glacial acetic acid solution from being washed excessively and completely discolored.
6. The method of claim 4, further comprising, in step g: and f, dehydrating the paraffin sections obtained in the step f in 95% alcohol for 30s, and dehydrating in absolute alcohol for 1 min.
7. The method according to claim 4, characterized in that it further comprises a step h of: and g, immersing the paraffin sections obtained in the step g in tap water for 2h for decoloring, and then re-dyeing the decolored paraffin sections according to the steps a-g.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201911335046.8A CN110926911A (en) | 2019-12-23 | 2019-12-23 | Pinus massoniana dyeing kit and dyeing method thereof |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201911335046.8A CN110926911A (en) | 2019-12-23 | 2019-12-23 | Pinus massoniana dyeing kit and dyeing method thereof |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| CN110926911A true CN110926911A (en) | 2020-03-27 |
Family
ID=69861709
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN201911335046.8A Pending CN110926911A (en) | 2019-12-23 | 2019-12-23 | Pinus massoniana dyeing kit and dyeing method thereof |
Country Status (1)
| Country | Link |
|---|---|
| CN (1) | CN110926911A (en) |
Cited By (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN118090377A (en) * | 2024-04-28 | 2024-05-28 | 北京大学第一医院(北京大学第一临床医学院) | Kit and method for special staining of kidney puncture tissue biopsy lens |
Citations (9)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN101897328A (en) * | 2010-01-11 | 2010-12-01 | 中国疾病预防控制中心寄生虫病预防控制所 | Method for staining protozoon sample |
| CN107050050A (en) * | 2017-04-19 | 2017-08-18 | 樊均明 | A kind of method for building up of the rhesus macaque chronic nephropathy model of glomerulus and renal tubule complex injury |
| CN107519153A (en) * | 2017-09-29 | 2017-12-29 | 四川省人民医院 | A kind of method that administration by gavage establishes rhesus macaque Liver Fibrosis Model |
| CN107693507A (en) * | 2017-09-29 | 2018-02-16 | 四川省人民医院 | A kind of method that intraperitoneal injection establishes rhesus macaque Liver Fibrosis Model |
| CN107907397A (en) * | 2017-11-23 | 2018-04-13 | 苏州泽科生物科技有限公司 | Collagenous fibres horse pine trichrome stain kit and preparation method thereof and colouring method |
| CN108543068A (en) * | 2018-05-30 | 2018-09-18 | 同济大学 | Application of the interleukin 37 in that modulates fibrosis relevant disease |
| CN108619149A (en) * | 2018-05-30 | 2018-10-09 | 同济大学 | Applications of the SIS3 in that modulates fibrosis relevant disease |
| CN108853121A (en) * | 2018-09-18 | 2018-11-23 | 上海中医药大学 | Application of the Nomilin in the drug that preparation improves hepar damnification caused by cholestasis and metabolic disease |
| CN109679929A (en) * | 2019-01-08 | 2019-04-26 | 安徽医科大学 | A kind of recombined adhenovirus, preparation method, application |
-
2019
- 2019-12-23 CN CN201911335046.8A patent/CN110926911A/en active Pending
Patent Citations (9)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN101897328A (en) * | 2010-01-11 | 2010-12-01 | 中国疾病预防控制中心寄生虫病预防控制所 | Method for staining protozoon sample |
| CN107050050A (en) * | 2017-04-19 | 2017-08-18 | 樊均明 | A kind of method for building up of the rhesus macaque chronic nephropathy model of glomerulus and renal tubule complex injury |
| CN107519153A (en) * | 2017-09-29 | 2017-12-29 | 四川省人民医院 | A kind of method that administration by gavage establishes rhesus macaque Liver Fibrosis Model |
| CN107693507A (en) * | 2017-09-29 | 2018-02-16 | 四川省人民医院 | A kind of method that intraperitoneal injection establishes rhesus macaque Liver Fibrosis Model |
| CN107907397A (en) * | 2017-11-23 | 2018-04-13 | 苏州泽科生物科技有限公司 | Collagenous fibres horse pine trichrome stain kit and preparation method thereof and colouring method |
| CN108543068A (en) * | 2018-05-30 | 2018-09-18 | 同济大学 | Application of the interleukin 37 in that modulates fibrosis relevant disease |
| CN108619149A (en) * | 2018-05-30 | 2018-10-09 | 同济大学 | Applications of the SIS3 in that modulates fibrosis relevant disease |
| CN108853121A (en) * | 2018-09-18 | 2018-11-23 | 上海中医药大学 | Application of the Nomilin in the drug that preparation improves hepar damnification caused by cholestasis and metabolic disease |
| CN109679929A (en) * | 2019-01-08 | 2019-04-26 | 安徽医科大学 | A kind of recombined adhenovirus, preparation method, application |
Non-Patent Citations (1)
| Title |
|---|
| 中华医学会: "《临床技术操作规范 病理学分册》", 30 April 2004, pages: 130 - 132 * |
Cited By (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN118090377A (en) * | 2024-04-28 | 2024-05-28 | 北京大学第一医院(北京大学第一临床医学院) | Kit and method for special staining of kidney puncture tissue biopsy lens |
Similar Documents
| Publication | Publication Date | Title |
|---|---|---|
| Azaripour et al. | A survey of clearing techniques for 3D imaging of tissues with special reference to connective tissue | |
| Jones et al. | Connective tissues and stains | |
| Bancroft et al. | Connective and other mesenchymal tissues with their stains | |
| CN106644656B (en) | One step decoration method of Hematoxylin-eosin | |
| Lison | Alcian blue 8 G with chlorantine fast red 5 B. A technic for selective staining of mucopolysaccharides | |
| CN105651580B (en) | A kind of dyed blended liquid in haematoxylin Yihong | |
| CN107490511A (en) | A kind of haematoxylin dyeing liquid and HE colouring methods | |
| CN104389032B (en) | A kind of Degumming method being applicable to containing green fluorescent protein silk | |
| CN110926911A (en) | Pinus massoniana dyeing kit and dyeing method thereof | |
| CN102876081A (en) | Hematoxylin staining solution and papanicolaou staining kit containing same | |
| CN103808550A (en) | Myxosporean dyeing and sealed-storage method convenient for morphological observation | |
| CN103525119A (en) | Cartilage staining solution, bone staining method and method for preparing embryonic bone specimen by using bone staining method | |
| CN109612807A (en) | A kind of urinary formed element dyeing liquor | |
| CN107488424B (en) | A kind of no benzene mountant and preparation method thereof | |
| CN110361244A (en) | The haematoxylin dye liquor and colouring method of slice are rapidly frozen for linear coloring instrument | |
| CN101897328B (en) | Method for staining protozoon sample | |
| CN103808553B (en) | Weak background fluorescence type resin embedding method | |
| CN107907397A (en) | Collagenous fibres horse pine trichrome stain kit and preparation method thereof and colouring method | |
| KR20170119936A (en) | A refractive index matching composition for biological tissue | |
| CN109900537A (en) | A kind of processing method of histological tissue specimen | |
| CN109632434A (en) | The Coomassie brilliant blue rapid dye liquor and dyeing-decolorzing method of a kind of low stimulation of low toxicity | |
| CN110926909A (en) | Papanicolaou staining kit and staining method thereof | |
| RU2593343C1 (en) | Method for staining the histological cuts in diagnosing trichinosis | |
| CN108168984B (en) | Protein PAGE gel electrophoresis rapid staining kit and staining method | |
| CN102749243B (en) | Method for staining nucleuses of shrimp embryos |
Legal Events
| Date | Code | Title | Description |
|---|---|---|---|
| PB01 | Publication | ||
| PB01 | Publication | ||
| CB02 | Change of applicant information |
Address after: Unit 306, B1 Floor, 218 Xinghu Street, Suzhou Industrial Park, Jiangsu Province Applicant after: Suzhou kansel medical laboratory Co.,Ltd. Address before: Unit 306, B1 Floor, 218 Xinghu Street, Suzhou Industrial Park, Jiangsu Province Applicant before: SUZHOU CANCERCELL BIOTECHNOLOGY Co.,Ltd. |
|
| CB02 | Change of applicant information | ||
| SE01 | Entry into force of request for substantive examination | ||
| SE01 | Entry into force of request for substantive examination | ||
| RJ01 | Rejection of invention patent application after publication |
Application publication date: 20200327 |
|
| RJ01 | Rejection of invention patent application after publication |