CN110923184A - Biological agent for repairing threonine fermentation wastewater - Google Patents
Biological agent for repairing threonine fermentation wastewater Download PDFInfo
- Publication number
- CN110923184A CN110923184A CN201911209512.8A CN201911209512A CN110923184A CN 110923184 A CN110923184 A CN 110923184A CN 201911209512 A CN201911209512 A CN 201911209512A CN 110923184 A CN110923184 A CN 110923184A
- Authority
- CN
- China
- Prior art keywords
- biological agent
- organic bentonite
- clostridium perfringens
- biological
- candida utilis
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Pending
Links
- 239000002351 wastewater Substances 0.000 title claims abstract description 34
- 239000003124 biologic agent Substances 0.000 title claims abstract description 32
- 238000000855 fermentation Methods 0.000 title claims description 26
- 230000004151 fermentation Effects 0.000 title claims description 25
- 239000004473 Threonine Substances 0.000 title claims description 22
- AYFVYJQAPQTCCC-UHFFFAOYSA-N Threonine Natural products CC(O)C(N)C(O)=O AYFVYJQAPQTCCC-UHFFFAOYSA-N 0.000 title claims description 21
- 239000000440 bentonite Substances 0.000 claims abstract description 39
- 229910000278 bentonite Inorganic materials 0.000 claims abstract description 39
- SVPXDRXYRYOSEX-UHFFFAOYSA-N bentoquatam Chemical compound O.O=[Si]=O.O=[Al]O[Al]=O SVPXDRXYRYOSEX-UHFFFAOYSA-N 0.000 claims abstract description 39
- 241000193468 Clostridium perfringens Species 0.000 claims abstract description 33
- 241000235646 Cyberlindnera jadinii Species 0.000 claims abstract description 31
- 238000002360 preparation method Methods 0.000 claims abstract description 10
- 239000007788 liquid Substances 0.000 claims description 30
- VEXZGXHMUGYJMC-UHFFFAOYSA-N Hydrochloric acid Chemical compound Cl VEXZGXHMUGYJMC-UHFFFAOYSA-N 0.000 claims description 16
- 238000012258 culturing Methods 0.000 claims description 15
- 239000000843 powder Substances 0.000 claims description 13
- 238000000034 method Methods 0.000 claims description 12
- 238000003756 stirring Methods 0.000 claims description 12
- 239000001963 growth medium Substances 0.000 claims description 11
- 238000002156 mixing Methods 0.000 claims description 10
- 239000002245 particle Substances 0.000 claims description 10
- 241000209094 Oryza Species 0.000 claims description 8
- 235000007164 Oryza sativa Nutrition 0.000 claims description 8
- 235000009566 rice Nutrition 0.000 claims description 8
- XLYOFNOQVPJJNP-UHFFFAOYSA-N water Substances O XLYOFNOQVPJJNP-UHFFFAOYSA-N 0.000 claims description 8
- XSQUKJJJFZCRTK-UHFFFAOYSA-N Urea Chemical compound NC(N)=O XSQUKJJJFZCRTK-UHFFFAOYSA-N 0.000 claims description 7
- 239000004202 carbamide Substances 0.000 claims description 7
- 238000001035 drying Methods 0.000 claims description 6
- 238000011081 inoculation Methods 0.000 claims description 5
- 239000007222 ypd medium Substances 0.000 claims description 5
- 238000001816 cooling Methods 0.000 claims description 4
- 238000010438 heat treatment Methods 0.000 claims description 4
- 238000009630 liquid culture Methods 0.000 claims description 4
- 239000002609 medium Substances 0.000 claims description 4
- 238000005469 granulation Methods 0.000 claims description 2
- 230000003179 granulation Effects 0.000 claims description 2
- 238000005067 remediation Methods 0.000 claims 1
- 239000003344 environmental pollutant Substances 0.000 abstract description 17
- 231100000719 pollutant Toxicity 0.000 abstract description 17
- 244000005700 microbiome Species 0.000 abstract description 6
- 230000007613 environmental effect Effects 0.000 abstract description 5
- 238000001179 sorption measurement Methods 0.000 abstract description 5
- 230000007246 mechanism Effects 0.000 abstract description 3
- FFEARJCKVFRZRR-BYPYZUCNSA-N L-methionine Chemical compound CSCC[C@H](N)C(O)=O FFEARJCKVFRZRR-BYPYZUCNSA-N 0.000 abstract description 2
- 229930182817 methionine Natural products 0.000 abstract description 2
- AYFVYJQAPQTCCC-GBXIJSLDSA-N L-threonine Chemical compound C[C@@H](O)[C@H](N)C(O)=O AYFVYJQAPQTCCC-GBXIJSLDSA-N 0.000 description 19
- 229960002898 threonine Drugs 0.000 description 19
- 230000000694 effects Effects 0.000 description 7
- XKMRRTOUMJRJIA-UHFFFAOYSA-N ammonia nh3 Chemical compound N.N XKMRRTOUMJRJIA-UHFFFAOYSA-N 0.000 description 5
- QVGXLLKOCUKJST-UHFFFAOYSA-N atomic oxygen Chemical compound [O] QVGXLLKOCUKJST-UHFFFAOYSA-N 0.000 description 5
- 230000001580 bacterial effect Effects 0.000 description 5
- 239000001301 oxygen Substances 0.000 description 5
- 229910052760 oxygen Inorganic materials 0.000 description 5
- 238000001914 filtration Methods 0.000 description 4
- 230000004048 modification Effects 0.000 description 4
- 238000012986 modification Methods 0.000 description 4
- 230000008569 process Effects 0.000 description 4
- 239000002994 raw material Substances 0.000 description 4
- 240000004808 Saccharomyces cerevisiae Species 0.000 description 3
- 244000249201 Scenedesmus obliquus Species 0.000 description 3
- 235000007122 Scenedesmus obliquus Nutrition 0.000 description 3
- VYPSYNLAJGMNEJ-UHFFFAOYSA-N Silicium dioxide Chemical compound O=[Si]=O VYPSYNLAJGMNEJ-UHFFFAOYSA-N 0.000 description 3
- 239000002131 composite material Substances 0.000 description 3
- 238000005516 engineering process Methods 0.000 description 3
- 230000000813 microbial effect Effects 0.000 description 3
- 238000004062 sedimentation Methods 0.000 description 3
- 239000000741 silica gel Substances 0.000 description 3
- 229910002027 silica gel Inorganic materials 0.000 description 3
- WWUZIQQURGPMPG-UHFFFAOYSA-N (-)-D-erythro-Sphingosine Natural products CCCCCCCCCCCCCC=CC(O)C(N)CO WWUZIQQURGPMPG-UHFFFAOYSA-N 0.000 description 2
- QJZYHAIUNVAGQP-UHFFFAOYSA-N 3-nitrobicyclo[2.2.1]hept-5-ene-2,3-dicarboxylic acid Chemical compound C1C2C=CC1C(C(=O)O)C2(C(O)=O)[N+]([O-])=O QJZYHAIUNVAGQP-UHFFFAOYSA-N 0.000 description 2
- 241000588626 Acinetobacter baumannii Species 0.000 description 2
- 239000005995 Aluminium silicate Substances 0.000 description 2
- IJGRMHOSHXDMSA-UHFFFAOYSA-N Atomic nitrogen Chemical compound N#N IJGRMHOSHXDMSA-UHFFFAOYSA-N 0.000 description 2
- 244000063299 Bacillus subtilis Species 0.000 description 2
- 235000014469 Bacillus subtilis Nutrition 0.000 description 2
- 241000894006 Bacteria Species 0.000 description 2
- 229920002101 Chitin Polymers 0.000 description 2
- 240000001046 Lactobacillus acidophilus Species 0.000 description 2
- 235000013956 Lactobacillus acidophilus Nutrition 0.000 description 2
- 239000002202 Polyethylene glycol Substances 0.000 description 2
- 241000223252 Rhodotorula Species 0.000 description 2
- CZMRCDWAGMRECN-UGDNZRGBSA-N Sucrose Chemical compound O[C@H]1[C@H](O)[C@@H](CO)O[C@@]1(CO)O[C@@H]1[C@H](O)[C@@H](O)[C@H](O)[C@@H](CO)O1 CZMRCDWAGMRECN-UGDNZRGBSA-N 0.000 description 2
- 229930006000 Sucrose Natural products 0.000 description 2
- 230000004075 alteration Effects 0.000 description 2
- 235000012211 aluminium silicate Nutrition 0.000 description 2
- BFNBIHQBYMNNAN-UHFFFAOYSA-N ammonium sulfate Chemical compound N.N.OS(O)(=O)=O BFNBIHQBYMNNAN-UHFFFAOYSA-N 0.000 description 2
- 229910052921 ammonium sulfate Inorganic materials 0.000 description 2
- 235000011130 ammonium sulphate Nutrition 0.000 description 2
- 230000015556 catabolic process Effects 0.000 description 2
- 239000000356 contaminant Substances 0.000 description 2
- 239000013078 crystal Substances 0.000 description 2
- 238000006731 degradation reaction Methods 0.000 description 2
- 238000009826 distribution Methods 0.000 description 2
- 239000003337 fertilizer Substances 0.000 description 2
- 239000004021 humic acid Substances 0.000 description 2
- NLYAJNPCOHFWQQ-UHFFFAOYSA-N kaolin Chemical compound O.O.O=[Al]O[Si](=O)O[Si](=O)O[Al]=O NLYAJNPCOHFWQQ-UHFFFAOYSA-N 0.000 description 2
- 229940039695 lactobacillus acidophilus Drugs 0.000 description 2
- 239000000203 mixture Substances 0.000 description 2
- 229920001223 polyethylene glycol Polymers 0.000 description 2
- 239000011148 porous material Substances 0.000 description 2
- WWUZIQQURGPMPG-KRWOKUGFSA-N sphingosine Chemical compound CCCCCCCCCCCCC\C=C\[C@@H](O)[C@@H](N)CO WWUZIQQURGPMPG-KRWOKUGFSA-N 0.000 description 2
- 239000005720 sucrose Substances 0.000 description 2
- 241000186063 Arthrobacter Species 0.000 description 1
- 241000193744 Bacillus amyloliquefaciens Species 0.000 description 1
- 241000193403 Clostridium Species 0.000 description 1
- 241000195493 Cryptophyta Species 0.000 description 1
- 102000004190 Enzymes Human genes 0.000 description 1
- 108090000790 Enzymes Proteins 0.000 description 1
- 241000589516 Pseudomonas Species 0.000 description 1
- 241000316848 Rhodococcus <scale insect> Species 0.000 description 1
- 230000003213 activating effect Effects 0.000 description 1
- 239000000654 additive Substances 0.000 description 1
- 230000009286 beneficial effect Effects 0.000 description 1
- 238000005842 biochemical reaction Methods 0.000 description 1
- 230000007227 biological adhesion Effects 0.000 description 1
- 229940086351 carbon dioxide 7 % Drugs 0.000 description 1
- 238000006243 chemical reaction Methods 0.000 description 1
- 150000001875 compounds Chemical class 0.000 description 1
- 230000007547 defect Effects 0.000 description 1
- 238000007599 discharging Methods 0.000 description 1
- 230000037149 energy metabolism Effects 0.000 description 1
- 238000000227 grinding Methods 0.000 description 1
- 239000001257 hydrogen Substances 0.000 description 1
- 229910052739 hydrogen Inorganic materials 0.000 description 1
- 125000004435 hydrogen atom Chemical class [H]* 0.000 description 1
- 125000002887 hydroxy group Chemical group [H]O* 0.000 description 1
- 230000007774 longterm Effects 0.000 description 1
- 238000004519 manufacturing process Methods 0.000 description 1
- 238000005374 membrane filtration Methods 0.000 description 1
- 230000002503 metabolic effect Effects 0.000 description 1
- 230000037353 metabolic pathway Effects 0.000 description 1
- 229910052757 nitrogen Inorganic materials 0.000 description 1
- 239000003895 organic fertilizer Substances 0.000 description 1
- 238000000053 physical method Methods 0.000 description 1
- 238000001556 precipitation Methods 0.000 description 1
- 230000035755 proliferation Effects 0.000 description 1
- 230000000644 propagated effect Effects 0.000 description 1
- 238000000746 purification Methods 0.000 description 1
- 229920006395 saturated elastomer Polymers 0.000 description 1
- 239000000126 substance Substances 0.000 description 1
- 238000006467 substitution reaction Methods 0.000 description 1
- 125000000341 threoninyl group Chemical group [H]OC([H])(C([H])([H])[H])C([H])(N([H])[H])C(*)=O 0.000 description 1
- 238000005303 weighing Methods 0.000 description 1
Images
Classifications
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N1/00—Microorganisms, e.g. protozoa; Compositions thereof; Processes of propagating, maintaining or preserving microorganisms or compositions thereof; Processes of preparing or isolating a composition containing a microorganism; Culture media therefor
- C12N1/36—Adaptation or attenuation of cells
-
- C—CHEMISTRY; METALLURGY
- C01—INORGANIC CHEMISTRY
- C01B—NON-METALLIC ELEMENTS; COMPOUNDS THEREOF; METALLOIDS OR COMPOUNDS THEREOF NOT COVERED BY SUBCLASS C01C
- C01B33/00—Silicon; Compounds thereof
- C01B33/20—Silicates
- C01B33/36—Silicates having base-exchange properties but not having molecular sieve properties
- C01B33/38—Layered base-exchange silicates, e.g. clays, micas or alkali metal silicates of kenyaite or magadiite type
- C01B33/40—Clays
-
- C—CHEMISTRY; METALLURGY
- C02—TREATMENT OF WATER, WASTE WATER, SEWAGE, OR SLUDGE
- C02F—TREATMENT OF WATER, WASTE WATER, SEWAGE, OR SLUDGE
- C02F1/00—Treatment of water, waste water, or sewage
- C02F1/001—Processes for the treatment of water whereby the filtration technique is of importance
-
- C—CHEMISTRY; METALLURGY
- C02—TREATMENT OF WATER, WASTE WATER, SEWAGE, OR SLUDGE
- C02F—TREATMENT OF WATER, WASTE WATER, SEWAGE, OR SLUDGE
- C02F3/00—Biological treatment of water, waste water, or sewage
- C02F3/34—Biological treatment of water, waste water, or sewage characterised by the microorganisms used
- C02F3/341—Consortia of bacteria
-
- C—CHEMISTRY; METALLURGY
- C02—TREATMENT OF WATER, WASTE WATER, SEWAGE, OR SLUDGE
- C02F—TREATMENT OF WATER, WASTE WATER, SEWAGE, OR SLUDGE
- C02F3/00—Biological treatment of water, waste water, or sewage
- C02F3/34—Biological treatment of water, waste water, or sewage characterised by the microorganisms used
- C02F3/347—Use of yeasts or fungi
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N11/00—Carrier-bound or immobilised enzymes; Carrier-bound or immobilised microbial cells; Preparation thereof
- C12N11/14—Enzymes or microbial cells immobilised on or in an inorganic carrier
-
- C—CHEMISTRY; METALLURGY
- C02—TREATMENT OF WATER, WASTE WATER, SEWAGE, OR SLUDGE
- C02F—TREATMENT OF WATER, WASTE WATER, SEWAGE, OR SLUDGE
- C02F2103/00—Nature of the water, waste water, sewage or sludge to be treated
- C02F2103/34—Nature of the water, waste water, sewage or sludge to be treated from industrial activities not provided for in groups C02F2103/12 - C02F2103/32
- C02F2103/36—Nature of the water, waste water, sewage or sludge to be treated from industrial activities not provided for in groups C02F2103/12 - C02F2103/32 from the manufacture of organic compounds
Landscapes
- Life Sciences & Earth Sciences (AREA)
- Chemical & Material Sciences (AREA)
- Organic Chemistry (AREA)
- Engineering & Computer Science (AREA)
- Microbiology (AREA)
- Health & Medical Sciences (AREA)
- Zoology (AREA)
- Wood Science & Technology (AREA)
- Genetics & Genomics (AREA)
- Bioinformatics & Cheminformatics (AREA)
- Biotechnology (AREA)
- Environmental & Geological Engineering (AREA)
- Water Supply & Treatment (AREA)
- Hydrology & Water Resources (AREA)
- General Engineering & Computer Science (AREA)
- General Health & Medical Sciences (AREA)
- Biodiversity & Conservation Biology (AREA)
- Biomedical Technology (AREA)
- Biochemistry (AREA)
- Inorganic Chemistry (AREA)
- Dispersion Chemistry (AREA)
- Mycology (AREA)
- Cell Biology (AREA)
- Medicinal Chemistry (AREA)
- Tropical Medicine & Parasitology (AREA)
- Virology (AREA)
- Purification Treatments By Anaerobic Or Anaerobic And Aerobic Bacteria Or Animals (AREA)
- Micro-Organisms Or Cultivation Processes Thereof (AREA)
- Water Treatment By Sorption (AREA)
Abstract
The invention belongs to the technical field of biological environmental protection, and discloses a biological agent for repairing fermented wastewater of methionine, which is prepared according to the following steps: step 1) modifying organic bentonite, step 2) domesticating clostridium perfringens, step 3) domesticating candida utilis, and step 4) preparing a biological preparation. The biological agent uses two kinds of microorganisms, treats pollutants through different mechanisms, can quickly remove the pollutants by matching with a carrier with high adsorption performance, can be repeatedly used for many times, continues to treat wastewater, and greatly saves the cost.
Description
Biological agent for repairing threonine fermentation wastewater
Technical Field
The invention belongs to the technical field of biological environmental protection, and particularly relates to a biological agent for repairing threonine fermentation wastewater.
Background
The environmental protection treatment of the threonine fermentation wastewater is a technical difficulty faced by threonine production enterprises, and various large enterprises and research institutes have conducted long-term research on the environmental protection treatment of the wastewater. The applicant's prior granted patent technology "CN 105948400B" discloses an environmental protection process for remediating L-threonine fermentation wastewater, which uses a combination of a physical method and a biological agent treatment method, the biological agent comprising the following raw materials in the following volume ratio: 6-7 parts of arthrobacter, 5-7 parts of pseudomonas, 5-7 parts of rhodococcus, 4-5 parts of bacillus amyloliquefaciens, 4-5 parts of clostridium papyriferum and 3-4 parts of scenedesmus obliquus; the microbial composite fertilizer contains various microorganisms with excellent degradation capability on pollutants difficult to degrade, and the strains are reasonably compatible, so that the microbial composite fertilizer has a good degradation effect and a wide application prospect. The applicant's prior granted patent technology "CN 105036351B" discloses a compound biological agent for treating threonine fermentation wastewater, which is prepared according to the following method: 1) weighing the following raw materials in parts by weight for later use, wherein the raw materials comprise 20-25 parts of water, 8-10 parts of polyethylene glycol, 7-8 parts of humic acid, 5-7 parts of sucrose, 3-4 parts of kaolin, 2-3 parts of ammonium sulfate, 2-3 parts of chitin and 1-2 parts of silica gel powder; preferably, the particle size of the silica gel powder is 200 meshes; 2) crushing humic acid by using a crusher, adding kaolin, uniformly mixing, and grinding to powder of 100 meshes; 3) sequentially adding water, polyethylene glycol, sucrose, ammonium sulfate, chitin and silica gel powder into a stirring reactor, stirring for 10min at 200 r/min, then adding the powder obtained in the step 2), stirring for 3min at 500 r/min, standing for 12h, and finally drying at 60 ℃ until the water content is lower than 5% (by weight), thus obtaining the biological carrier; 4) respectively culturing bacillus subtilis, lactobacillus acidophilus, acinetobacter baumannii and sphingosine monospore bacteria to obtain bacterial liquid with the concentration of 1 × 107/ml, culturing scenedesmus obliquus to obtain algae liquid with the concentration of 1 × 105/ml, and mixing the bacillus subtilis bacterial liquid, the lactobacillus acidophilus bacterial liquid, the acinetobacter baumannii liquid, the sphingosine monospore bacterial liquid and the scenedesmus obliquus liquid according to the ratio of 5: 3: 2: 1:1, and standing for 6 hours to obtain a mixed liquid; 5) mixing the mixed liquid with the biological carrier according to the ratio of 1: 2, uniformly mixing and stirring, fermenting at room temperature for 24-36h, and drying until the water content is 10% (by weight); the biological carrier of the composite biological agent has large specific surface area and good biological adhesion, and the compatibility of each microorganism is reasonable, thus improving the capability of treating wastewater. However, the culture process is complicated for many types of microorganisms contained in the above biological preparations, and once one microorganism is contaminated, the whole biological preparation process is stopped, which also increases the burden of enterprises.
Disclosure of Invention
The invention aims to overcome the defects of the prior art and provides a biological agent for repairing the wastewater from the fermentation of methionine, the biological agent uses two microorganisms, pollutants are treated by different mechanisms, the pollutants can be quickly removed by matching with a carrier with high adsorption performance, the biological agent can be repeatedly used for many times, the wastewater is continuously treated, and the cost is greatly saved.
In order to achieve the purpose of the invention, the following technical scheme is adopted.
The biological agent for repairing threonine fermentation wastewater is prepared by the following steps:
step 1) modifying organic bentonite, step 2) domesticating clostridium perfringens, step 3) domesticating candida utilis, and step 4) preparing a biological preparation.
Further, the biological agent is prepared according to the following steps:
step 1) modifying the organic bentonite: adding the organic bentonite into a hydrochloric acid solution, uniformly stirring, heating to 50-60 ℃, preserving heat for 30-60min, then adding rice hull powder and urea, uniformly stirring, then entering a wet-process granulator for granulation to obtain particles with the particle size of 2-4mm, then roasting at the temperature of 550 ℃ for 600 ℃ for 1h, taking out, and cooling to room temperature to obtain the modified organic bentonite;
step 2) domesticating clostridium perfringens: inoculating the candida utilis seed solution into an acclimatization culture medium according to the inoculation amount of 10%, and carrying out acclimatization culture for 12h at the temperature of 30 ℃ to obtain candida utilis acclimatization solution;
step 3) domestication of candida utilis: inoculating the clostridium perfringens seed liquid into an acclimatization culture medium according to the inoculation amount of 10%, and carrying out acclimatization culture for 12h at 33 ℃ to obtain clostridium perfringens acclimatization liquid;
step 4) preparing a biological agent: mixing Clostridium perfringens acclimation solution and modified organic bentonite, culturing for 6h under anaerobic condition, adding Candida utilis acclimation solution, culturing for 6h, drying at 20 deg.C until water content is 15-20%, and preserving at 4 deg.C.
Preferably, the concentration of the hydrochloric acid solution is 0.5-1M.
Preferably, the organobentonite is present in an amount of 1 g: 1-2ml of hydrochloric acid solution.
Preferably, the rice hull powder accounts for 20-40% of the organic bentonite in parts by mass.
Preferably, the urea accounts for 3-8% of the mass of the organic bentonite.
Preferably, the acclimatization medium used in the step 2) consists of: the volume ratio of the YPD medium to the fermentation wastewater was 1: 1.
Preferably, the acclimatization medium used in the step 3) consists of: the volume ratio of the FTG liquid culture medium to the fermentation wastewater is 1: 1.
Preferably, in the step 4), the ratio of the clostridium perfringens domestication liquid to the candida utilis domestication liquid to the modified organic bentonite is 1 ml: 1 ml: 1-3 g.
Compared with the prior art, the invention mainly has the following advantages that:
the organic bentonite is prepared by the steps of (1) activating the organic bentonite firstly, removing partial hydroxyl groups to form broken bonds so as to enhance the activity, adding a certain amount of additives, namely rice hull powder and urea, and then roasting, wherein the crystal structure and the crystal form of the organic bentonite are changed and become orderly arranged to form floccules of a cluster, the surface of the organic bentonite becomes loose and rough to form more porous structures, the pore distribution is more uniform, the specific surface area of the organic bentonite is improved, the organic bentonite has better pollutant adsorption performance and is beneficial to the attachment of strains;
the clostridium perfringens is an anaerobic bacterium, lacks a complete metabolic enzyme system, the energy metabolism is carried out in an anaerobic fermentation mode, the oxygen content in the wastewater is low, and the strain is wrapped in a carrier and cannot be contacted with oxygen, so that the proliferation and anaerobic biochemical reaction of the strain are facilitated; the candida utilis is facultative anaerobe, comprises two metabolic pathways, and can be propagated and biochemically reacted under the conditions of sufficient oxygen and insufficient oxygen; in view of the property of candida utilis, the candida utilis is coated on the surface of the modified organic bentonite, so that pollutants can be fermented under aerobic and anaerobic conditions, and clostridium perfringens in the modified organic bentonite degrades the pollutants under the anaerobic condition; the two strains are attached to different parts of the modified organic bentonite according to different requirements on oxygen, can be symbiotically coordinated, and treat pollutants through different mechanisms.
After the biological agent disclosed by the invention is used for treating the wastewater, the liquid is discharged by filtering, and the biological agent is continuously kept in the treatment tank, so that the biological agent can be repeatedly used for a plurality of times, the wastewater is continuously treated, and the cost is greatly saved.
Drawings
FIG. 1: influence of different strain combination modes on the removal rate of COD;
FIG. 2: influence of different strain combination modes on the removal rate of ammonia nitrogen;
FIG. 3: influence of different strain combinations on SS removal rate.
Detailed Description
Those skilled in the art can modify the process parameters appropriately to achieve the desired results with reference to the disclosure herein. It is expressly intended that all such similar substitutes and modifications which would be obvious to one skilled in the art are deemed to be included in the invention. While the products and methods of this invention have been described in terms of preferred embodiments, it will be apparent to those of skill in the art that variations and modifications, or appropriate alterations and combinations, of the products and methods described herein may be made and utilized without departing from the spirit, scope, and spirit of the invention. For a further understanding of the present invention, reference will now be made in detail to the following examples.
Example 1
The biological agent for repairing threonine fermentation wastewater is prepared by the following steps:
1) mixing organic bentonite according to the proportion of 1 g: adding 2ml of the mixture into 0.5M hydrochloric acid, uniformly stirring, heating to 55 ℃, preserving heat for 30min, adding 30 mass parts of rice hull powder and 5 mass parts of urea in the organic bentonite, uniformly stirring, granulating in a wet granulator to obtain particles with the particle size of 2mm, roasting at 550 ℃ for 1h, taking out, and cooling to room temperature to obtain the modified organic bentonite;
2) mixing Clostridium perfringens acclimation solution and modified organic bentonite, culturing for 6h under anaerobic condition, adding Candida utilis acclimation solution, culturing for 6h, drying at 20 deg.C until water content is 15%, and preserving at 4 deg.C; the ratio of the clostridium perfringens domestication liquid to the candida utilis domestication liquid to the modified organic bentonite is 1 ml: 1 ml: 2g of the total weight.
The preparation method of the candida utilis domestication liquid comprises the following steps: selecting Candida utilis (ATCC22023) single colony, inoculating into YPD medium, culturing at 30 deg.C and 200rpm for 12 hr, transferring into 1L YPD medium, culturing at 30 deg.C and 200rpm for 24 hr, inoculating into 10L YPD medium at 5%, and culturing to 1 × 109cfu/ml to obtain candida utilis seed liquid; inoculating the seed solution into acclimatization culture medium (YPD culture medium and fermentation wastewater volume ratio of 1: 1) at 10%, acclimatizing and culturing at 30 deg.C for 12 hr to obtain proteinCandida domestication liquid;
the preparation method of the clostridium perfringens domestication liquid comprises the following steps: inoculating Clostridium perfringens (ATCC 13124) into FTG liquid culture medium, placing into 33 deg.C anaerobic incubator (nitrogen 87%, carbon dioxide 7%, hydrogen 6%), culturing under anaerobic condition until the concentration is 1 × 108cfu/ml, and obtaining clostridium perfringens seed liquid; inoculating the strain into an acclimatization culture medium (volume ratio of FTG liquid culture medium to fermentation wastewater is 1: 1) according to the inoculation amount of 10%, and carrying out acclimatization culture at 33 ℃ for 12h to obtain the clostridium perfringens acclimatization solution.
Example 2
The biological agent for repairing threonine fermentation wastewater is prepared by the following steps:
1) mixing organic bentonite according to the weight ratio of 2 g: adding 3ml of the mixture into 1M hydrochloric acid, uniformly stirring, heating to 60 ℃, preserving heat for 30min, adding rice hull powder accounting for 35 mass percent of the organic bentonite and urea accounting for 6 mass percent of the organic bentonite, uniformly stirring, granulating in a wet granulator to obtain particles with the particle size of 3mm, roasting at 600 ℃ for 1h, taking out, and cooling to room temperature to obtain the organic bentonite-based organic fertilizer;
2) mixing Clostridium perfringens acclimation solution and modified organic bentonite, culturing for 6h under anaerobic condition, adding Candida utilis acclimation solution, culturing for 6h, drying at 25 deg.C until water content is 15%, and preserving at 4 deg.C; the ratio of the clostridium perfringens domestication liquid to the candida utilis domestication liquid to the modified organic bentonite is 1 ml: 1 ml: 3g of the total weight.
Example 3
The purification process of the threonine fermentation wastewater, which uses the biological agent, specifically comprises the following steps:
the threonine fermentation wastewater is wastewater generated in the process of preparing threonine by using a microbial fermentation technology, and the generation mode is as follows: separating the mycoprotein in the threonine fermentation liquor by using a high-speed disc separator, and collecting threonine feed liquid; extracting threonine from threonine feed liquid by membrane filtration, concentration and isoelectric precipitation, wherein the produced wastewater is threonine fermentation wastewater;
filtering large-particle floating substances from the threonine fermentation wastewater by a mechanical fence; then naturally settling in a sedimentation tank, adjusting the pH value to be 6-7, and finally entering a biological reaction tank, wherein the weight ratio of the raw materials is 100 g: 1m3Adding biological agent according to the proportion, treating for 48h, filtering by a filter screen with the aperture of 1mm, and discharging.
Example 4
1. The effect of different combinations of strains on major contaminants.
Group 1: only candida utilis is adopted;
group 2: clostridium perfringens + candida utilis;
group 3: clostridium perfringens + rhodosporidium palustum;
group 4: clostridium perfringens + angel yeast.
Indexes of main pollutants of the wastewater subjected to sedimentation treatment in the sedimentation tank are as follows: COD is 3689mg/L, ammonia nitrogen is 475mg/L, SS is 389mg/L, effluent indexes after the filtration of the filter screen are monitored, and removal rates of three main pollutants of COD, ammonia nitrogen and SS are calculated.
As shown in fig. 1-3, different strain combination modes find that the combination mode of clostridium perfringens and candida utilis has the best effect of removing COD and ammonia nitrogen, the removal rate of SS is 95.5% while the effect is better because the removal rate of 48h can reach more than 98%, and the treatment time is continuously increased, so that the effect of removing pollutants is not greatly influenced; only adopting the candida utilis, prolonging the treatment time, and approaching the maximum value in 60h, probably because the candida utilis can not effectively and quickly treat pollutants in time, so that the carrier is saturated and the pollutants can not be adsorbed more; by adopting a combination mode of clostridium perfringens, rhodosporidium palustum, clostridium perfringens and Angel yeast, the removal peak value of pollutants is similar to that of clostridium perfringens and candida utilis and is about 48h, the removal rate of SS is approximate to that of the combination of clostridium perfringens and candida utilis, but the removal effect of COD and ammonia nitrogen can only reach about 90 percent and is lower than that of the combination of clostridium perfringens and candida utilis. It can be seen that there is a large difference in the removal of major contaminants when different yeast types and clostridium perfringens are combined.
2. The influence of the modification mode of the organic bentonite on the performance.
Mode 1: unmodified organobentonite;
mode 2: the same procedure as in example 1 was repeated except that the treatment was not carried out;
mode 3: the rest of the procedure was the same as in example 1, except that no rice hull powder was added;
mode 4: inventive example 1.
The density and specific surface area indexes of the four types of organobentonite are shown in table 1.
TABLE 1
| High density g/cm | Specific surface area m2/g | |
| Mode 1 | 1.71 | 23.5 |
| Mode 2 | 1.03 | 64.1 |
| Mode 3 | 1.29 | 56.9 |
| Mode 4 | 1.05 | 97.2 |
And (4) conclusion: according to the invention, the surface of the organic bentonite is loosened and rough through modification treatment, more porous structures are formed, the pore distribution is uniform, the specific surface area is improved, the pollutant adsorption performance is better, the bacterial strain attachment is facilitated, and the organic bentonite is suspended and dispersed in liquid, so that the pollutant adsorption range can be increased.
Although the present invention has been described with reference to the preferred embodiments, it should be understood that various changes, substitutions and alterations can be made herein without departing from the spirit and scope of the invention as defined by the appended claims.
Claims (10)
1. The biological agent for repairing threonine fermentation wastewater is prepared by the following steps:
step 1) modifying organic bentonite, step 2) domesticating clostridium perfringens, step 3) domesticating candida utilis, and step 4) preparing a biological preparation.
2. The biological agent according to claim 1, wherein the biological agent is prepared by the following steps:
step 1) modifying the organic bentonite: adding the organic bentonite into a hydrochloric acid solution, uniformly stirring, heating to 50-60 ℃, preserving heat for 30-60min, then adding rice hull powder and urea, uniformly stirring, then entering a wet-process granulator for granulation to obtain particles with the particle size of 2-4mm, then roasting at the temperature of 550 ℃ for 600 ℃ for 1h, taking out, and cooling to room temperature to obtain the modified organic bentonite;
step 2) domesticating clostridium perfringens: inoculating the candida utilis seed solution into an acclimatization culture medium according to the inoculation amount of 10%, and carrying out acclimatization culture for 12h at the temperature of 30 ℃ to obtain candida utilis acclimatization solution;
step 3) domestication of candida utilis: inoculating the clostridium perfringens seed liquid into an acclimatization culture medium according to the inoculation amount of 10%, and carrying out acclimatization culture for 12h at 33 ℃ to obtain clostridium perfringens acclimatization liquid;
step 4) preparing a biological agent: mixing Clostridium perfringens acclimation solution and modified organic bentonite, culturing for 6h under anaerobic condition, adding Candida utilis acclimation solution, culturing for 6h, drying at 20 deg.C until water content is 15-20%, and preserving at 4 deg.C.
3. The biological agent according to claim 2, wherein the hydrochloric acid solution has a concentration of 0.5 to 1M.
4. The biological agent according to claim 2, characterized in that the organobentonite has a molar mass according to 1 g: 1-2ml of hydrochloric acid solution.
5. The biological agent according to claim 2, wherein the rice hull powder accounts for 20 to 40 mass% of the organobentonite.
6. The biological agent according to claim 2, wherein the urea is present in an amount of 3 to 8% by mass based on the total mass of the organic bentonite.
7. The biological preparation according to claim 2, wherein the acclimatization medium used in step 2) consists of: the volume ratio of the YPD medium to the fermentation wastewater was 1: 1.
8. The biological preparation according to claim 2, wherein the acclimatization medium used in step 3) consists of: the volume ratio of the FTG liquid culture medium to the fermentation wastewater is 1: 1.
9. The biological preparation according to claim 2, wherein in the step 4), the ratio of the clostridium perfringens domestication liquid to the candida utilis domestication liquid to the modified organic bentonite is 1 ml: 1 ml: 1-3 g.
10. Use of the biological agent of claims 1-9 for the remediation of threonine fermentation wastewater.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201911209512.8A CN110923184A (en) | 2019-12-01 | 2019-12-01 | Biological agent for repairing threonine fermentation wastewater |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201911209512.8A CN110923184A (en) | 2019-12-01 | 2019-12-01 | Biological agent for repairing threonine fermentation wastewater |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| CN110923184A true CN110923184A (en) | 2020-03-27 |
Family
ID=69848187
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN201911209512.8A Pending CN110923184A (en) | 2019-12-01 | 2019-12-01 | Biological agent for repairing threonine fermentation wastewater |
Country Status (1)
| Country | Link |
|---|---|
| CN (1) | CN110923184A (en) |
Citations (13)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| US20090208470A1 (en) * | 2005-10-22 | 2009-08-20 | Se-Joon Park | Microorganisms Having Bad Smell Removal Activity of Organic Waste and Use Thereof |
| CN104649763A (en) * | 2015-03-03 | 2015-05-27 | 徐州工程学院 | Anaerobic treatment method of excreta in intensive cattle farm |
| CN105645587A (en) * | 2016-02-24 | 2016-06-08 | 杭州富阳高博信息技术服务有限公司 | Biological agent for treatment of industrial polluted water |
| CN107988111A (en) * | 2017-12-26 | 2018-05-04 | 江苏星海生物科技有限公司 | A kind of complex microbial inoculum for handling farm's pollutant and its application |
| WO2018133411A1 (en) * | 2017-01-17 | 2018-07-26 | 广州市广深环保科技有限公司 | Multifunctional and efficient sewage microorganism activated bacterial agent and use thereof |
| CN109679867A (en) * | 2018-12-27 | 2019-04-26 | 呼伦贝尔东北阜丰生物科技有限公司 | The separating and extracting process of span amino acid mother liquor |
| CN110092529A (en) * | 2019-04-03 | 2019-08-06 | 卢松 | Utilize the technique of bacterial strain glycolysis Amino Acid Fermentation Wastewater |
| CN110092530A (en) * | 2019-04-03 | 2019-08-06 | 卢松 | A kind of biochemical preparation and preparation method thereof purifying fermentation waste water |
| CN110240285A (en) * | 2019-06-19 | 2019-09-17 | 广东睿洁环保工程有限公司 | A kind of composite bacteria agent reducing COD value in serious pollution waste water |
| CN110331105A (en) * | 2019-07-11 | 2019-10-15 | 湖北真福医药有限公司 | A kind of sewage treatment composite bacteria agent and preparation method thereof |
| CN110902950A (en) * | 2019-12-02 | 2020-03-24 | 齐齐哈尔龙江阜丰生物科技有限公司 | Treatment method of starch industrial wastewater |
| CN110921841A (en) * | 2019-12-02 | 2020-03-27 | 齐齐哈尔龙江阜丰生物科技有限公司 | Biochemical preparation for repairing starch industrial wastewater |
| CN110981082A (en) * | 2019-12-01 | 2020-04-10 | 齐齐哈尔龙江阜丰生物科技有限公司 | Purification process of threonine fermentation wastewater |
-
2019
- 2019-12-01 CN CN201911209512.8A patent/CN110923184A/en active Pending
Patent Citations (13)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| US20090208470A1 (en) * | 2005-10-22 | 2009-08-20 | Se-Joon Park | Microorganisms Having Bad Smell Removal Activity of Organic Waste and Use Thereof |
| CN104649763A (en) * | 2015-03-03 | 2015-05-27 | 徐州工程学院 | Anaerobic treatment method of excreta in intensive cattle farm |
| CN105645587A (en) * | 2016-02-24 | 2016-06-08 | 杭州富阳高博信息技术服务有限公司 | Biological agent for treatment of industrial polluted water |
| WO2018133411A1 (en) * | 2017-01-17 | 2018-07-26 | 广州市广深环保科技有限公司 | Multifunctional and efficient sewage microorganism activated bacterial agent and use thereof |
| CN107988111A (en) * | 2017-12-26 | 2018-05-04 | 江苏星海生物科技有限公司 | A kind of complex microbial inoculum for handling farm's pollutant and its application |
| CN109679867A (en) * | 2018-12-27 | 2019-04-26 | 呼伦贝尔东北阜丰生物科技有限公司 | The separating and extracting process of span amino acid mother liquor |
| CN110092529A (en) * | 2019-04-03 | 2019-08-06 | 卢松 | Utilize the technique of bacterial strain glycolysis Amino Acid Fermentation Wastewater |
| CN110092530A (en) * | 2019-04-03 | 2019-08-06 | 卢松 | A kind of biochemical preparation and preparation method thereof purifying fermentation waste water |
| CN110240285A (en) * | 2019-06-19 | 2019-09-17 | 广东睿洁环保工程有限公司 | A kind of composite bacteria agent reducing COD value in serious pollution waste water |
| CN110331105A (en) * | 2019-07-11 | 2019-10-15 | 湖北真福医药有限公司 | A kind of sewage treatment composite bacteria agent and preparation method thereof |
| CN110981082A (en) * | 2019-12-01 | 2020-04-10 | 齐齐哈尔龙江阜丰生物科技有限公司 | Purification process of threonine fermentation wastewater |
| CN110902950A (en) * | 2019-12-02 | 2020-03-24 | 齐齐哈尔龙江阜丰生物科技有限公司 | Treatment method of starch industrial wastewater |
| CN110921841A (en) * | 2019-12-02 | 2020-03-27 | 齐齐哈尔龙江阜丰生物科技有限公司 | Biochemical preparation for repairing starch industrial wastewater |
Non-Patent Citations (6)
| Title |
|---|
| XIAONAN ZHOU等: "Effect of pH on volatile fatty acid production and the microbial community during anaerobic digestion of Chinese cabbage waste", 《BIORESOURCE TECHNOLOGY》 * |
| 冶贵生等: "餐厨废水中A型产气荚膜梭菌的分离与鉴定", 《黑龙江畜牧兽医》 * |
| 励飞等: "一株发酵木薯酒糟的热带假丝酵母菌株的分离及营养分析", 《中国酿造》 * |
| 周媛等: "高浓度发酵废水生产单细胞蛋白的菌种筛选", 《环境工程学报》 * |
| 肖娟等: "膨润土改性及应用进展", 《当代化工》 * |
| 赵秀文等: "酵母菌在食品工业废水处理中的应用现状", 《中国酿造》 * |
Similar Documents
| Publication | Publication Date | Title |
|---|---|---|
| CN110981082B (en) | Purification process of threonine fermentation wastewater | |
| CN108624582B (en) | Microbial preparation for soil remediation | |
| CN102465105B (en) | Nitrous acid-type denitrification bacteria strain and application thereof | |
| CN113444661B (en) | Sphingobacterium neoformans and application thereof in wastewater dephosphorization | |
| CN105060495B (en) | A kind of biological agent for repairing high ammonia nitrogen trade effluent | |
| CN110577336B (en) | Harmless treatment method for enhancing flocculation of aquaculture tail water | |
| CN112408607B (en) | Application of staphylococcus nepalensis in degradation of residual sugar in fermentation industrial organic wastewater | |
| CN104628225A (en) | Treatment method of ammonia-nitrogen-containing industrial wastewater | |
| CN102583772A (en) | Microbial preparation comprising mixed microorganisms (bm-s-1), and a biological treatment method for rivers and lakes and a sludge autodigestion process using the same | |
| CN107473404B (en) | Water purifying agent with self-formed block-shaped carbon carrier for fixing microorganisms and preparation method thereof | |
| CN105000674A (en) | Method used for processing environmental geology industrial wastewater via biotechnology | |
| Rong et al. | Towards advanced mariculture wastewater treatment by bacterial-algal symbiosis system with different bacteria and algae inoculation ratios | |
| CN110902950B (en) | Treatment method of starch industrial wastewater | |
| CN113151010B (en) | Microbial compound bacterium preparation and preparation method thereof | |
| CN106967644B (en) | Biological agent for treating glutamic acid fermentation sewage | |
| CN102465106B (en) | Bacterial strain used for short-cut denitrification for nitrogen removal and its application | |
| CN110921841B (en) | Biochemical preparation for repairing starch industrial wastewater | |
| CN108975494B (en) | Method for treating black smelly water by using graphene modified straw material | |
| CN112481249A (en) | Preparation method of immobilized biocatalyst | |
| CN112080442A (en) | Special microbial inoculum for ectopic treatment of livestock and poultry manure and preparation method thereof | |
| CN109468251B (en) | Thiourea degrading strain and method for treating thiourea-containing wastewater by using same | |
| CN108570325B (en) | Microbial preparation for high-salinity soil remediation and preparation method thereof | |
| CN109055259B (en) | Pseudomonas XD-3, application thereof and microbial flocculant | |
| CN114958669B (en) | Michigan klebsiella and product and application thereof | |
| CN110923184A (en) | Biological agent for repairing threonine fermentation wastewater |
Legal Events
| Date | Code | Title | Description |
|---|---|---|---|
| PB01 | Publication | ||
| PB01 | Publication | ||
| SE01 | Entry into force of request for substantive examination | ||
| SE01 | Entry into force of request for substantive examination | ||
| WD01 | Invention patent application deemed withdrawn after publication |
Application publication date: 20200327 |
|
| WD01 | Invention patent application deemed withdrawn after publication |