CN110923132A

CN110923132A - Escherichia coli fermentation liquor concentration system

Info

Publication number: CN110923132A
Application number: CN201911336829.8A
Authority: CN
Inventors: 费逸; 王志伟; 朱城
Original assignee: Individual
Current assignee: Individual
Priority date: 2019-12-23
Filing date: 2019-12-23
Publication date: 2020-03-27

Abstract

The invention relates to the field of biological preparation, in particular to an escherichia coli fermentation liquor concentration system which comprises a cover body, wherein the cover body comprises a cover plate, a sampling pipe, a material adding pipe, a first connector, a material discharging pipe, a fixing plate, an exhaust pipe, an electromagnetic valve, a filter box, a barometer, an air inlet pipe, an air pump connector, a first sealing ring, a first threaded ring, a first glass inner wall, a defoaming plate and a first ceramic membrane, the sampling pipe, the material adding pipe, the material discharging pipe, the exhaust pipe and the air inlet pipe penetrate through the cover plate, the barometer is arranged on the cover plate, and the first sealing ring is hermetically connected at the joint of the sampling pipe, the material adding pipe, the first connector, the exhaust pipe, the baro. The invention is in the shape of a kettle body consisting of a cover body, a supporting mechanism, a middle cylinder and a bottom mechanism, a ceramic membrane is arranged in the kettle body to concentrate fermentation liquor, the process is simple and easy to operate, and the addition and the recovery of the fermentation liquor and the treatment and the recovery of water are completed by specific structures.

Description

Escherichia coli fermentation liquor concentration system

Technical Field

The invention relates to the field of biological preparation, in particular to an escherichia coli fermentation liquor concentration system.

Background

The detection method of the escherichia coli generally comprises a fermentation method, a filter membrane method, a plate counting method, an immunomagnetic bead method, an automatic instrument detection method and an ATP bioluminescence method, wherein the fermentation method needs fermentation liquor special for the escherichia coli.

The traditional concentration of the escherichia coli fermentation liquor is generally completed by utilizing a ceramic membrane, most of the existing concentration devices are only simple and are connected with the structure of the ceramic membrane, and the related structures or steps of how to treat filtered water or liquid and how to recover the fermentation liquor are not clearly replaced, so that the technical defects exist.

Disclosure of Invention

Aiming at the problems in the prior art, the invention provides an escherichia coli fermentation liquor concentration system.

The technical scheme adopted by the invention for solving the technical problems is as follows: an escherichia coli fermentation liquor concentration system comprises a cover body, wherein the cover body comprises a cover plate, a sampling pipe, a feeding pipe, a connector, a discharging pipe, a fixing plate, an exhaust pipe, an electromagnetic valve, a filter box, a barometer, an air inlet pipe, an air pump connector, a sealing ring, a threaded ring, a glass inner wall, a defoaming plate and a ceramic membrane, the cover plate is provided with the sampling pipe, the feeding pipe, the discharging pipe, the exhaust pipe and the air inlet pipe in a penetrating manner, the barometer is arranged on the cover plate, the sampling pipe, the feeding pipe, the connector, the exhaust pipe, the barometer and the joint of the air inlet pipe and the cover plate are all connected with the sealing ring in a sealing manner, the sampling pipe and the sealing ring are connected in a sealing manner, the upper end of the feeding pipe is connected with the connector in a sealing manner, the discharging pipe is fixedly connected with the, the utility model discloses a filter box, including blast pipe, filter box, air pump connector, the lower extreme of blast pipe, sealing connection has the filter box on the lower extreme of blast pipe, sealing welding has a screw ring on the lower side board of apron, all scribble a glass inner wall on the inner wall of a screw ring and apron, the filter box is kept away from fixedly connected with defoaming board on a screw ring inner wall of blast pipe one side, quantity be two, and install respectively on adding material pipe and discharging pipe, the lid is connected on supporting mechanism's last port, supporting mechanism's lower port is connected bottom mechanism through a middle part section of thick bamboo, No. two sealing washers and No. three sealing washers are held to the centre of the body between supporting mechanism and the middle part section of thick bamboo and between a middle part section of thick bamboo and the bottom mechanism.

Specific, supporting mechanism includes go-between, seal ring, No. two screw rings, backing ring, No. two glass inner walls, internal thread and stabilizer blade, the last port of go-between is equipped with the seal ring, the welding has No. two screw rings on the go-between inner wall of seal ring below, No. two screw rings are kept away from and are equipped with the backing ring on the go-between of seal ring one side, scribble on the inboard of backing ring and be equipped with No. two glass inner walls, the backing ring is kept away from the go-between port department of No. two screw rings one side and is equipped with the internal thread No. one, the outside welding of go-between has the three stabilizer blade that is the setting of article font.

The ceramic film packaging barrel is characterized in that the middle barrel comprises a barrel body, an external thread, a frame body, a second connector, a third connector, a second ceramic film, a second frame body, a third glass inner wall and a second internal thread, the external thread is arranged on the outer side of an upper port of the barrel body, two connectors are connected to the inner wall of the upper port of the barrel body through the frame body, two connectors are connected to the two connectors in a one-to-one mode, the two ceramic films are fixed to the inner wall of the barrel body through the two frame bodies, the two ceramic films are connected to the third connector, a third glass inner wall is coated on the inner wall of the barrel body, and the second internal thread is arranged on the inner side of the lower port of the barrel body.

The bottom mechanism comprises a kettle body, two external threads, a third frame body, a fourth connector, a third ceramic membrane, a fifth connector, a communicating pipe, a bottom filter screen, a pump frame, a waste discharge pump machine and a fourth glass inner wall, wherein the second external threads are arranged on the outer side of the port on the kettle body, two fourth connectors are connected to the inner wall of the upper port of the kettle body through the third frame body, two ceramic membranes are connected to the fourth connector in a one-to-one correspondence manner, one end, away from the fourth connector, of the third ceramic membrane is connected with the fifth connector, the two fourth connectors are connected through the communicating pipe, the bottom filter screen is fixedly connected to the inner wall of the kettle body below the communicating pipe, the bottom of the kettle body is fixedly connected with the pump frame, a waste discharge pump machine is fixed in the pump frame, and the inlet end of the waste discharge pump machine is arranged in the kettle body.

Specifically, No. two sealing washer centre grippings are between go-between and apron, No. three sealing washer centre grippings are under a screw ring between the side and the last side of backing ring, No. four sealing washers centre gripping respectively between the downside of backing ring and the last port of barrel and between the lower port of barrel and the last port of the cauldron body.

Specifically, threaded connection between a threaded ring and No. two threaded rings, threaded connection between an internal thread and an external thread, threaded connection between No. two internal threads and No. three support bodies.

It is specific, two the No. two connectors correspond grafting, two with adding material pipe and discharging pipe respectively No. three the connector corresponds the grafting with two No. four connectors respectively.

Specifically, all scribble on the inner wall of a screw ring and apron and be equipped with a glass inner wall, scribble on the inboard of backing ring and be equipped with No. two glass inner walls, scribble on the inner wall of barrel and be equipped with No. three glass inner walls, scribble on the inner wall of the cauldron body and be equipped with No. four glass inner walls.

The invention has the beneficial effects that:

(1) the cover plate of the cover body is provided with an air inlet pipe and an exhaust pipe for regulating internal pressure, an air pressure meter for monitoring the internal pressure, a material adding pipe and a material discharging pipe for feeding and discharging, and a sampling pipe for sampling in the concentration process, the design ensures that the cover plate has basic functions of regulating internal air pressure, sampling in the concentration process, feeding and discharging, the port of the sampling pipe can be plugged by a rubber plug, and a ceramic membrane arranged on the material adding pipe and the material discharging pipe can concentrate fermentation liquor, and the design ensures that the cover plate has the function of concentrating the fermentation liquor; the cover body and the middle barrel are connected together through the supporting mechanism, and the design ensures that the cover is simple to disassemble and assemble, and is convenient for a user to clean the inside of the cover.

(2) The upper connecting structure and the lower connecting structure of the middle cylinder are matched with each other, the number of the middle cylinders is increased according to the requirement, and the concentration efficiency of the ceramic membrane ceramic; the bottom mechanism has a final effect, a ceramic membrane series connection channel has a waste liquid pumping and discharging function, and the design of the bottom filter screen prevents the mistakenly-entering impurities from entering the waste pump.

Drawings

The invention is further illustrated with reference to the following figures and examples.

FIG. 1 is a schematic structural diagram of an Escherichia coli fermentation broth concentration system provided by the present invention;

FIG. 2 is a schematic diagram of a cover structure of an Escherichia coli fermentation broth concentration system according to the present invention;

FIG. 3 is a schematic structural diagram of a supporting mechanism of an Escherichia coli fermentation broth concentration system provided by the present invention;

FIG. 4 is a schematic structural diagram of a middle cylinder of an Escherichia coli fermentation broth concentration system provided by the present invention;

FIG. 5 is a schematic structural diagram of a bottom mechanism of an Escherichia coli fermentation broth concentration system provided by the present invention;

FIG. 6 is a schematic view of a connection ring structure of an Escherichia coli fermentation broth concentration system according to the present invention;

FIG. 7 is a schematic view of a connection structure of the legs of the Escherichia coli fermentation broth concentration system according to the present invention.

In the figure: 1. a cover body; 11. a cover plate; 12. a sampling tube; 13. a feeding pipe; 14. a first connector; 15. a discharge pipe; 16. a fixing plate; 17. an exhaust pipe; 18. an electromagnetic valve; 19. a filter box; 110. a barometer; 111. an air inlet pipe; 112. an air pump connector; 113. a first sealing ring; 114. a first threaded ring; 115. the inner wall of the first glass; 116. a defoaming plate; 117. a first ceramic membrane; 2. a support mechanism; 21. a connecting ring; 22. a seal ring; 23. a second threaded ring; 24. a backing ring; 25. the inner wall of the second glass; 26. a first internal thread; 27. a support leg; 3. a middle barrel; 31. a barrel; 32. a first external thread; 33. a first frame body; 34. a second connector; 35. a third connector; 36. a second ceramic membrane; 37. a second frame body; 38. the inner wall of the third glass; 39. a second internal thread; 4. a bottom mechanism; 41. a kettle body; 42. a second external thread; 43. a third frame body; 44. a fourth connector; 45. a third ceramic membrane; 46. a fifth connector; 47. a communicating pipe; 48. a bottom screen; 49. a pump housing; 410. a waste discharge pump; 411. the inner wall of No. four glass; 5. a second sealing ring; 6. a third sealing ring; 7. and a fourth sealing ring.

Detailed Description

In order to make the technical means, the creation characteristics, the achievement purposes and the effects of the invention easy to understand, the invention is further described with the specific embodiments.

As shown in fig. 1-7, the escherichia coli fermentation broth concentration system comprises a cover body 1, wherein the cover body 1 comprises a cover plate 11, a sampling tube 12, a feeding tube 13, a first connector 14, a discharging tube 15, a fixing plate 16, an exhaust tube 17, an electromagnetic valve 18, a filter box 19, a barometer 110, an air inlet tube 111, an air pump connector 112, a first sealing ring 113, a first threaded ring 114, a first glass inner wall 115, a defoaming plate 116 and a first ceramic membrane 117, the cover plate 11 is provided with the sampling tube 12, the feeding tube 13, the discharging tube 15, the exhaust tube 17 and the air inlet tube 111 in a penetrating manner, the barometer 110 is arranged on the cover plate 11, the sampling tube 12, the feeding tube 13, the first connector 14, the exhaust tube 17, the barometer 110 and the joint of the air inlet tube 111 and the cover plate 11 are all connected with the first sealing ring 113 in a sealing manner, the sampling tube 12 and the first sealing ring 113, the upper end of the feeding pipe 13 is hermetically connected with a first connecting head 14, the discharging pipe 15 is fixedly connected with the cover plate 11 through a fixing plate 16, the upper end of the discharging pipe 17 is connected with an electromagnetic valve 18, the lower end of the discharging pipe 17 is hermetically connected with a filter box 19, the upper end of the gas inlet pipe 111 is hermetically connected with a gas pump connecting head 112, the lower side panel of the cover plate 11 is hermetically welded with a first threaded ring 114, the inner walls of the first threaded ring 114 and the cover plate 11 are coated with a first glass inner wall 115, the inner wall of the first threaded ring 114, far away from the discharging pipe 17, of the filter box 19 is fixedly connected with a defoaming plate 116, the number of the first threaded ring 117 is two, the first threaded ring and the defoaming plate are respectively arranged on the feeding pipe 13 and the discharging pipe 15, the cover body 1 is connected with the upper port of the supporting mechanism 2, and the lower port of the supporting, a second sealing ring 5 and a third sealing ring 6 are clamped between the cover body 1 and the supporting mechanism 2, and a fourth sealing ring 7 is clamped between the supporting mechanism 2 and the middle cylinder 3 and between the middle cylinder 3 and the bottom mechanism 4.

Specific, supporting mechanism 2 includes go-between 21, sealing gasket insert ring 22, No. two screw rings 23, backing ring 24, No. two glass inner walls 25, internal thread 26 and stabilizer blade 27, the last port of go-between 21 is equipped with sealing gasket insert ring 22, the welding has No. two screw rings 23 on the go-between 21 inner wall of sealing gasket insert ring 22 below, No. two screw rings 23 are kept away from and are equipped with backing ring 24 on the go-between 21 of sealing gasket insert ring 22 one side, No. two glass inner walls 25 have been scribbled on backing ring 24's the inboard, backing ring 24 keeps away from No. two screw rings 23 one side go-between 21 port department and is equipped with internal thread 26, the outside welding of go-between 21 has three stabilizer blade 27 that is the setting of article font.

It is specific, middle part section of thick bamboo 3 includes barrel 31, external screw thread 32, a support body 33, No. two connectors 34, No. three connectors 35, No. two ceramic membranes 36, No. two support bodies 37, No. three glass inner walls 38 and No. two internal threads 39, the last port outside of barrel 31 is equipped with an external screw thread 32, be connected with No. two connectors 34, two through a support body 33 on the last port inner wall of barrel 31 No. two connectors 34 one-to-one is connected with No. two ceramic membranes 36, No. two ceramic membranes 36 are fixed on the inner wall of barrel 31 through No. two support bodies 37, just No. two ceramic membranes 36 be connected with No. three connectors 35, scribble No. three glass inner walls 38 on the inner wall of barrel 31, the lower port inside of barrel 31 is equipped with No. two internal threads 39.

Specifically, the bottom mechanism 4 comprises a kettle body 41, a second external thread 42, a third frame body 43, a fourth connector 44, a third ceramic membrane 45, a fifth connector 46, a communicating pipe 47, a bottom filter screen 48, a pump frame 49, a waste discharge pump machine 410 and a fourth glass inner wall 411, wherein the second external thread 42 is arranged on the outer side of the upper port of the kettle body 41, two fourth connectors 44 are connected to the inner wall of the upper port of the kettle body 41 through the third frame body 43, the two fourth connectors 44 are connected with the third ceramic membrane 45 in a one-to-one correspondence manner, one end of the third ceramic membrane 45, which is far away from the fourth connector 44, is connected with the fifth connector 46, the two fourth connectors 44 are connected through the communicating pipe 47, the bottom filter screen 48 is fixedly connected to the inner wall of the kettle body 41 below the communicating pipe 47, the bottom of the kettle body 41 is fixedly connected with a pump frame 49, the waste discharge pump machine 410 is fixed in the pump frame 49, the inlet end of the waste discharge pump 410 is arranged in the kettle body 41, and a No. four glass inner wall 411 is coated on the inner wall of the kettle body 41.

Specifically, No. two sealing washer 5 centre grippings are between go-between 21 and apron 11, No. three sealing washer 6 centre grippings are under a screw ring 114 between the side and the side of going up of backing ring 24, No. four sealing washer 7 centre grippings respectively between the downside of backing ring 24 and the last port of barrel 31 and between the lower port of barrel 31 and the last port of cauldron body 41 to be convenient for sealed.

Specifically, threaded connection between threaded ring 114 and threaded ring two 23, threaded connection between internal thread 26 and external thread 32, threaded connection between internal thread 39 and No. three support body 43 to be connected.

Specifically, the two second connectors 34 are correspondingly inserted into the material adding pipe 13 and the material discharging pipe 15, and the two third connectors 35 are correspondingly inserted into the two fifth connectors 46, so that the operation of the invention is facilitated.

Specifically, the inner walls of the first threaded ring 114 and the cover plate 11 are coated with a first glass inner wall 115, the inner side of the backing ring 24 is coated with a second glass inner wall 25, the inner wall of the cylinder 31 is coated with a third glass inner wall 38, and the inner wall of the kettle body 41 is coated with a fourth glass inner wall 411 to reduce internal impurities.

When in use, the cover plate 11 of the cover body 1 designed by the invention is provided with an air inlet pipe 111 and an air outlet pipe 17 for regulating and controlling the internal pressure, a barometer 110 for monitoring the internal pressure, a feeding pipe 13 and a discharging pipe 15 for feeding and discharging, and a sampling pipe 12 for sampling in the concentration process, the design ensures that the invention has the basic functions of regulating and controlling the internal pressure, sampling in the concentration process, feeding and discharging, the port of the sampling pipe 12 can be plugged by a rubber plug, and a ceramic membrane 117 arranged on the feeding pipe 13 and the discharging pipe 15 can concentrate fermentation liquor, the design ensures that the invention has the function of concentrating the fermentation liquor, the supporting mechanism 2 designed by the invention is used for supporting the whole invention, and the cover body 1 and the middle cylinder are connected together, the design ensures that the disassembly and the assembly of the invention are simple, and the user can conveniently clean the inside of the invention, the upper connecting structure and the lower connecting structure of the middle cylinder designed by the invention are matched with each other, so that a user can increase the number of the middle cylinder 3 according to the requirement to adjust the concentration efficiency of the invention, in the process, the first external thread 32 and the second internal thread 39 at the two ends of the cylinder body 31 are matched with each other, and the splicing structure of the second connector 34 and the third connector 35 is matched with the specification, so that the middle cylinder 3 can be connected end to end, the height of the invention is prolonged, namely, the length of the ceramic membrane of the invention is increased, the concentration effect is increased, the bottom mechanism 4 designed by the invention has the ending effect, the ceramic membrane is connected in series and has the function of pumping and discharging waste liquid, the bottom filter screen 48 is designed to prevent the mistakenly-inserted impurities from entering the waste pump 410, and the invention is composed of a cover body 1 and a supporting mechanism 2, The middle barrel 3 and the bottom mechanism 4 form a kettle body shape, a ceramic membrane is arranged in the kettle body to concentrate fermentation liquor, the process is simple and easy to operate, and the addition and the recovery of the fermentation liquor and the treatment and the recovery of water are completed by specific structures.

The foregoing illustrates and describes the principles, general features, and advantages of the present invention. It will be understood by those skilled in the art that the present invention is not limited to the embodiments described above, and the embodiments and descriptions given above are only illustrative of the principles of the present invention, and various changes and modifications may be made without departing from the spirit and scope of the invention, which fall within the scope of the claims. The scope of the invention is defined by the appended claims and equivalents thereof.

Claims

1. An escherichia coli fermentation liquor concentration system is characterized in that: the novel vacuum glass cover comprises a cover body (1), wherein the cover body (1) comprises a cover plate (11), a sampling pipe (12), a feeding pipe (13), a first connector (14), a discharge pipe (15), a fixing plate (16), an exhaust pipe (17), an electromagnetic valve (18), a filter box (19), a barometer (110), an air inlet pipe (111), an air pump connector (112), a sealing ring (113), a threaded ring (114), a glass inner wall (115), a defoaming plate (116) and a ceramic membrane (117), the sampling pipe (12), the feeding pipe (13), the discharge pipe (15), the exhaust pipe (17) and the air inlet pipe (111) are arranged on the cover plate (11) in a penetrating manner, the barometer (110) is arranged on the cover plate (11), and the joint of the sampling pipe (12), the feeding pipe (13), the first connector (14), the exhaust pipe (17), the barometer (110) and the air inlet pipe (111) and the cover plate (11) is hermetically connected with a first, sealing connection between sampling tube (12) and sealing washer (113), add upper end sealing connection of material pipe (13) has connector (14) No. one, fixed connection between fixed plate (16) and apron (11) is passed through in discharging pipe (15), be connected with solenoid valve (18) on exhausting pipe (17) the upper end, sealing connection has cartridge (19) on the lower extreme of exhausting pipe (17), the upper end sealing connection of intake pipe (111) has air pump connector (112), sealing welding has a screw ring (114) on the lower side board of apron (11), fixedly connected with foam removing plate (116) on a screw ring (114) inner wall of exhausting pipe (17) one side is kept away from to cartridge (19), the quantity of a ceramic membrane (117) is two, and installs respectively on adding material pipe (13) and discharging pipe (15), lid (1) is connected on the upper port of supporting mechanism (2), the lower port of the supporting mechanism (2) is connected with a bottom mechanism (4) through a middle barrel (3), a second sealing ring (5) and a third sealing ring (6) are clamped between the cover body (1) and the supporting mechanism (2), and a fourth sealing ring (7) is clamped between the supporting mechanism (2) and the middle barrel (3) and between the middle barrel (3) and the bottom mechanism (4).

2. The system for concentrating fermentation liquid of escherichia coli according to claim 1, wherein: supporting mechanism (2) include go-between (21), seal ring insert (22), No. two screw rings (23), backing ring (24), No. two glass inner walls (25), internal thread (26) and stabilizer blade (27), the last port of go-between (21) is equipped with seal ring insert (22), the welding has No. two screw rings (23) on go-between (21) inner wall of seal ring insert (22) below, No. two screw rings (23) are kept away from and are equipped with backing ring (24) on go-between (21) of seal ring insert (22) one side, backing ring (24) are kept away from go-between (21) port department of No. two screw rings (23) one side and are equipped with internal thread (26) No. one, the outside welding of go-between (21) has three stabilizer blade (27) that are the setting of article font.

3. The system for concentrating fermentation liquid of escherichia coli according to claim 1, wherein: the middle barrel (3) comprises a barrel body (31), a first external thread (32), a first frame body (33), a second connector (34), a third connector (35), a second ceramic membrane (36), a second frame body (37), a third glass inner wall (38) and a second internal thread (39), the outer side of the upper port of the cylinder body (31) is provided with a first external thread (32), the inner wall of the upper port of the cylinder body (31) is connected with two second connectors (34) through a first frame body (33), the two second connectors (34) are correspondingly connected with second ceramic membranes (36) one by one, the second ceramic membrane (36) is fixed on the inner wall of the cylinder body (31) through a second frame body (37), and the second ceramic membrane (36) is connected with a third connector (35), and a second internal thread (39) is arranged on the inner side of the lower port of the cylinder body (31).

4. The system for concentrating fermentation liquid of escherichia coli according to claim 1, wherein: the bottom mechanism (4) comprises a kettle body (41), a second external thread (42), a third frame body (43), a fourth connector (44), a third ceramic membrane (45), a fifth connector (46), a communicating pipe (47), a bottom filter screen (48), a pump frame (49), a waste discharge pump machine (410) and a fourth glass inner wall (411), wherein the second external thread (42) is arranged on the outer side of an upper port of the kettle body (41), the inner wall of the upper port of the kettle body (41) is connected with the two fourth connectors (44) through the third frame body (43), the two fourth connectors (44) are correspondingly connected with the third ceramic membrane (45) one by one, one end of the third ceramic membrane (45) far away from the fourth connector (44) is connected with the fifth communicating pipe (46), the two fourth connectors (44) are connected through the connector (47), the bottom filter screen (48) is fixedly connected on the inner wall of the kettle body (41) below the communicating pipe (47), the bottom fixedly connected with pump frame (49) of the cauldron body (41), pump frame (49) internal fixation has waste discharge pump machine (410), the entrance point setting of waste discharge pump machine (410) is in the cauldron body (41).

5. The system for concentrating fermentation liquid of escherichia coli according to claim 1, wherein: no. two sealing washer (5) centre gripping is between go up ring (21) and apron (11), No. three sealing washer (6) centre gripping is between the side and the side of going up of backing ring (24) under threaded ring (114), No. four sealing washer (7) centre gripping respectively between the downside of backing ring (24) and the last port of barrel (31) and between the lower port of barrel (31) and the last port of the cauldron body (41).

6. The system for concentrating fermentation liquid of escherichia coli according to claim 1, wherein: threaded connection between a threaded ring (114) and No. two threaded ring (23), threaded connection between an internal thread (26) and an external thread (32), threaded connection between No. two internal threads (39) and No. three support bodies (43).

7. The system for concentrating fermentation liquid of escherichia coli according to claim 3, wherein: two No. two connector (34) correspond grafting, two with adding material pipe (13) and discharging pipe (15) respectively No. three connector (35) correspond grafting with two No. five connectors (46) respectively.

8. The system for concentrating fermentation liquid of escherichia coli according to claim 1, wherein: a glass inner wall (115) is all scribbled on the inner wall of a screw ring (114) and apron (11), No. two glass inner walls (25) are scribbled on the inboard of backing ring (24), No. three glass inner walls (38) are scribbled on the inner wall of barrel (31), No. four glass inner walls (411) are scribbled on the inner wall of the cauldron body (41).