CN110919706A - Cell precision cutting device and use method thereof - Google Patents
Cell precision cutting device and use method thereof Download PDFInfo
- Publication number
- CN110919706A CN110919706A CN201911061503.9A CN201911061503A CN110919706A CN 110919706 A CN110919706 A CN 110919706A CN 201911061503 A CN201911061503 A CN 201911061503A CN 110919706 A CN110919706 A CN 110919706A
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- Prior art keywords
- cutting device
- limiting plate
- cylinder
- transverse
- cell
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Classifications
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- B—PERFORMING OPERATIONS; TRANSPORTING
- B26—HAND CUTTING TOOLS; CUTTING; SEVERING
- B26D—CUTTING; DETAILS COMMON TO MACHINES FOR PERFORATING, PUNCHING, CUTTING-OUT, STAMPING-OUT OR SEVERING
- B26D1/00—Cutting through work characterised by the nature or movement of the cutting member or particular materials not otherwise provided for; Apparatus or machines therefor; Cutting members therefor
- B26D1/01—Cutting through work characterised by the nature or movement of the cutting member or particular materials not otherwise provided for; Apparatus or machines therefor; Cutting members therefor involving a cutting member which does not travel with the work
- B26D1/02—Cutting through work characterised by the nature or movement of the cutting member or particular materials not otherwise provided for; Apparatus or machines therefor; Cutting members therefor involving a cutting member which does not travel with the work having a stationary cutting member
- B26D1/03—Cutting through work characterised by the nature or movement of the cutting member or particular materials not otherwise provided for; Apparatus or machines therefor; Cutting members therefor involving a cutting member which does not travel with the work having a stationary cutting member with a plurality of cutting members
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- B—PERFORMING OPERATIONS; TRANSPORTING
- B26—HAND CUTTING TOOLS; CUTTING; SEVERING
- B26D—CUTTING; DETAILS COMMON TO MACHINES FOR PERFORATING, PUNCHING, CUTTING-OUT, STAMPING-OUT OR SEVERING
- B26D7/00—Details of apparatus for cutting, cutting-out, stamping-out, punching, perforating, or severing by means other than cutting
- B26D7/26—Means for mounting or adjusting the cutting member; Means for adjusting the stroke of the cutting member
- B26D7/2628—Means for adjusting the position of the cutting member
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- Life Sciences & Earth Sciences (AREA)
- Forests & Forestry (AREA)
- Engineering & Computer Science (AREA)
- Mechanical Engineering (AREA)
- Sampling And Sample Adjustment (AREA)
Abstract
The invention relates to the technical field of medical instruments, and discloses a cell precision cutting device and a using method thereof, wherein the cell precision cutting device comprises a separating cylinder and a first protective cover, the first protective cover is provided with a water inlet and a sampling port/liquid inlet, a combined type cutter is arranged in the separating cylinder, the combined type cutter comprises a frame body and a plurality of transverse blades, one sides of the transverse blades are rotatably connected to the frame body, the other sides of the transverse blades are connected with a linkage rod together, the top ends or the bottom ends of the transverse blades are provided with longitudinal blades, and the two ends of the longitudinal blades are both fixed with the inner side wall of the; a guide cylinder is fixed outside the frame body, a driving rod penetrates through the inner thread of the guide cylinder, one end of the driving rod is rotatably connected with the linkage rod, and the other end of the driving rod is a driving end. According to the technical scheme, the linkage rod drives the transverse blade to rotate, the size of the cutting opening between the transverse blade and the longitudinal blade is flexibly adjusted, and therefore the purpose of simply and conveniently changing the size of the cutting opening is achieved.
Description
Technical Field
The invention relates to the technical field of medical instruments, in particular to a cell precision cutting device and a using method thereof.
Background
The cell precision cutting device is mainly used for separating and cutting cells and obtaining tissue cells with different sizes. When tissue cells with different sizes need to be obtained, the current separating device needs to select and replace cutting tools with different mesh diameters so as to achieve the purpose of obtaining tissue cell groups with different sizes. When the cutting tools with different mesh diameters are replaced, the replacement is troublesome, the efficiency is low and the operation is extremely inconvenient in actual operation.
Disclosure of Invention
Aiming at the technical problem that the cutting tools with different mesh diameters are required to be replaced and complicated in the background technology, the first purpose of the invention is to provide a cell precision cutting device, wherein a linkage rod drives a transverse blade to rotate, so that the size of a cutting opening between the transverse blade and a longitudinal blade is flexibly adjusted, and the purpose of simply and conveniently changing the size of the cutting opening is achieved.
In order to achieve the first object, the invention provides the following technical scheme:
a cell precision cutting device comprises a separating cylinder and a first protective cover, wherein a water inlet and a sampling port/liquid inlet are formed in the first protective cover, a combined type cutter is arranged in the separating cylinder, the combined type cutter comprises a frame body and a plurality of transverse blades which are arranged in the frame body in parallel, one sides of the transverse blades are rotatably connected to the inner side wall of the frame body, the other sides of the transverse blades are connected with a linkage rod together, a longitudinal blade is arranged at the top end or the bottom end of each transverse blade, the two ends of each longitudinal blade are fixed to the inner side wall of the frame body, and a cutting port is formed between each transverse blade and each longitudinal blade;
the outer portion of the frame body is fixedly provided with a guide cylinder, a driving rod penetrates through an inner thread of the guide cylinder, one end of the driving rod is connected with the linkage rod in a rotating mode, the other end of the driving rod is a driving end, the driving end is rotated to drive the driving rod to move along the axial direction of the driving rod, and the linkage rod is driven to move back and forth to control the size of the cutting opening.
Through the technical scheme, the linkage rod drives the transverse blade to rotate, the size of the cutting opening between the transverse blade and the longitudinal blade is flexibly adjusted, and therefore the purpose of simply and conveniently changing the size of the cutting opening is achieved. The optional specification that its cutting opening size was adjusted sets gradually: 2.6mm, 2.4mm, 2.2mm, 1.8mm, 1.2mm, 0.8 mm.
The invention is further configured to: the week side of separator cylinder is equipped with the space that slides of indent, the guide cylinder is fixed in the space that slides, it has the groove of sliding to open in the space that slides, the guide cylinder slide install in slide on the groove.
Through above-mentioned technical scheme, the guide cylinder reciprocates in the inslot that slides, can adjust the mounting height of combined type cutter in the separator cylinder.
The invention is further configured to: be equipped with the limiting plate in the space that slides, the limiting plate includes last limiting plate and lower limiting plate, go up the limiting plate and all fix on the cylinder with lower limiting plate.
Through above-mentioned technical scheme, set up the limiting plate, include limiting plate and lower limiting plate, be convenient for carry on spacingly to the height of sliding of guide cylinder, prevent the skew of position of sliding.
The invention is further configured to: and a connecting plate is arranged between the upper limiting plate and the lower limiting plate.
Through above-mentioned technical scheme, set up the connecting plate, mainly will go up between spacing board and the lower spacing board and couple together for go up between spacing board and the lower spacing board connect fixedly more firm.
The invention is further configured to: the separating cylinder deviates from one end of the first protective cover is provided with a second protective cover.
Through above-mentioned technical scheme, set up the second visor, mainly make things convenient for to install and dismantle from the both ends of cylinder, improve the convenience of sealed cutting device when the in-service use.
The invention is further configured to: the second protective cover is also provided with a water inlet and a sampling port/liquid inlet.
Through above-mentioned technical scheme, set up the water inlet, conveniently add the primitive cell tissue liquid that treats the cutting in to the cylinder, set up sample connection/inlet, conveniently take out the sample from the cylinder to and return the sample that will take out from sample connection/inlet and pour into in the cylinder.
The invention is further configured to: and the driving end is fixedly connected with a driving wheel.
The invention is further configured to: the driving wheel is in a circular truncated cone shape, and the peripheral side of the driving wheel is provided with a strip edge for increasing friction force.
Through above-mentioned technical scheme, set up the drive wheel, and the drive wheel is round platform shape, sets up the strip arris of increase frictional force in the drive wheel week side of round platform shape for it is more convenient to rotate the drive wheel.
Based on the above-mentioned cell precision cutting device, the second objective of the present invention is to provide a precision cutting method, comprising the following steps:
s1: injecting cell tissue liquid to be cut into the separation cylinder through the water inlet;
s2: moving the guide cylinder to adjust the cutting height of the combined type cutter;
s3: the separating cylinder is shaken up and down to enable cell tissue fluid to impact and cut on the combined type cutter, so that the purpose of cutting is achieved;
s4: after a certain period of operation, samples were taken from the sample/liquid inlet and the results of the operation were checked.
Through the technical scheme.
In conclusion, the invention has the following beneficial effects:
(1) the linkage rod drives the transverse blade to rotate, so that the size of a cutting opening between the transverse blade and the longitudinal blade is flexibly adjusted, and the purpose of simply and conveniently changing the size of the cutting opening is achieved;
(2) the guide cylinder is arranged to move up and down in the sliding groove, so that the mounting height of the composite cutter in the separation cylinder can be adjusted;
(3) through setting up the second visor, mainly make things convenient for to install and dismantle from the both ends of separating cylinder, improve the convenience of sealed cutting device when the in-service use.
Drawings
FIG. 1 is a schematic diagram of the overall three-dimensional structure of a cell precision cutting device;
FIG. 2 is a schematic view of an overall split structure of the cell precision cutting device;
FIG. 3 is a schematic view of a split structure of the separation cylinder;
FIG. 4 is a schematic structural view of the composite cutter installed inside the separating cylinder;
FIG. 5 is a perspective view of the composite cutting tool;
FIG. 6 is an enlarged view of the structure at A in FIG. 5;
fig. 7 is a schematic view showing the installation structure of the guide cylinder and the driving rod on the frame body;
FIG. 8 is an enlarged view of the structure at B in FIG. 7;
fig. 9 is an enlarged view of the structure at C in fig. 7.
Reference numerals: 1. a separation cylinder; 11. a slippage space; 12. a sliding groove; 13. a limiting plate; 131. an upper limiting plate; 132. a lower limiting plate; 133. a connecting plate; 2. a first protective cover; 21. a water inlet; 22. a sample port/liquid inlet; 3. a composite cutter; 31. a frame body; 32. a transverse blade; 33. a longitudinal blade; 4. a linkage rod; 5. cutting the opening; 6. a guide cylinder; 7. a drive rod; 71. a driving end; 72. a drive wheel; 73. strip edges; 8. a second protective cover.
Detailed Description
The present invention will be described in further detail with reference to examples and drawings, but the present invention is not limited to these examples.
Example 1
The utility model provides a cell precision cutting device, as shown in fig. 1 and 2, includes separator 1 and first protective cover 2, and the one end that separator 1 deviates from first protective cover 2 is equipped with second visor 8, is equipped with water inlet 21 and sample connection/inlet 22 on the first protective cover 2, and also is equipped with water inlet 21 and sample connection/inlet 22 on the second visor 8 equally. As can be seen from fig. 3 and 4, the composite cutter 3 is disposed in the separation cylinder 1, and the composite cutter 3 includes a frame 31 and a plurality of transverse blades 32 disposed in parallel in the frame 31. Referring to fig. 5 and 6, one side of the transverse blade 32 is rotatably connected to the inner side wall of the frame 31, the other side of the transverse blade 32 is connected to the linkage rod 4, a longitudinal blade 33 is arranged at the top end or the bottom end of the transverse blade 32, both ends of the longitudinal blade 33 are fixed to the inner side wall of the frame 31, and a cutting opening 5 (see fig. 6) is formed between the transverse blade 32 and the longitudinal blade 33. Meanwhile, a guide cylinder 6 is fixed outside the frame body 31, a driving rod 7 penetrates through an internal thread of the guide cylinder 6, one end of the driving rod 7 is rotatably connected with the linkage rod 4, the other end of the driving rod 7 is a driving end 71, and the driving end 71 is rotated to drive the driving rod 7 to move along the axial direction of the driving rod and drive the linkage rod 4 to move back and forth to control the size of the cutting opening 5.
In order to adjust the installation height of the composite cutter 3 conveniently, an inwards concave sliding space 11 is arranged on the peripheral side of the separating cylinder 1, the guide cylinder 6 is fixed in the sliding space 11, a sliding groove 12 (see fig. 3) is formed in the sliding space 11, and the guide cylinder 6 is installed on the sliding groove 12 in a sliding manner.
As shown in fig. 3, a limiting plate 13 is arranged in the sliding space 11, the limiting plate 13 includes an upper limiting plate 131 and a lower limiting plate 132, the upper limiting plate 131 and the lower limiting plate 132 are both fixed on the separation cylinder 1, and a connecting plate 133 is arranged between the upper limiting plate 131 and the lower limiting plate 132. As can be seen from fig. 7 and 9, the driving wheel 72 is fixedly connected to the driving end 71, the driving wheel 72 is in a circular truncated cone shape, and a ridge 73 for increasing friction force is provided on the peripheral side of the driving wheel 72.
As can be seen from fig. 7 and 8, in particular, in order to mount the driving rod 7, the driving rod 7 can drive the linkage rod 4 to move back and forth when rotating, so as to achieve the purpose of adjustment. Therefore, the drive rod 7 is provided as a screw, and the drive rod 7 is screwed into the guide cylinder 6. Meanwhile, one end of the driving rod 7, which is far away from the driving wheel 72, is rotatably connected with the linkage rod 4, so that the driving rod 7 cannot drive the linkage rod 4 to rotate together when rotating.
Example 2
Based on the cell precision cutting device, a precision cutting method is provided, which comprises the following steps:
s1: cell tissue fluid to be cut is injected into the separation cylinder 1 through a water inlet 21;
s2: the cutting height of the combined type cutter 3 is adjusted by the movable guide cylinder 6;
s3: shaking the separating cylinder 1 up and down to make the cell tissue fluid impact and cut on the composite cutter 3 to achieve the purpose of cutting;
s4: after a certain period of operation, a sample is taken from the sample/liquid inlet 22 and the result of the operation is checked.
The above description is only a preferred embodiment of the present invention, and the protection scope of the present invention is not limited to the above embodiments, and all technical solutions belonging to the idea of the present invention belong to the protection scope of the present invention. It should be noted that modifications and embellishments within the scope of the invention may occur to those skilled in the art without departing from the principle of the invention, and are considered to be within the scope of the invention.
Claims (9)
1. A cell precision cutting device, which comprises a separating cylinder (1) and a first protective cover (2), the first protective cover (2) is provided with a water inlet (21) and a sampling port/liquid inlet (22), it is characterized in that a composite cutter (3) is arranged in the separating cylinder (1), the composite cutter (3) comprises a frame body (31) and a plurality of transverse blades (32) which are arranged in the frame body (31) in parallel, one side of the transverse blade (32) is rotatably connected to the inner side wall of the frame body (31), the other sides of the transverse blades (32) are connected with a linkage rod (4) together, the top end or the bottom end of the transverse blade (32) is provided with a longitudinal blade (33), both ends of the longitudinal blade (33) are fixed with the inner side wall of the frame body (31), a cutting opening (5) is formed between the transverse blade (32) and the longitudinal blade (33);
the outside of framework (31) is fixed with guide cylinder (6), actuating lever (7) are worn to be equipped with by guide cylinder (6) internal thread, the one end of actuating lever (7) with gangbar (4) rotate to be connected, the other end of actuating lever (7) is drive end (71), rotates drive end (71) drive actuating lever (7) are along its axial displacement, drive gangbar (4) back-and-forth movement control the size of cutting opening (5).
2. The precision cell cutting device according to claim 1, wherein: the week side of separator cylinder (1) is equipped with the space (11) that slides of indent, guide cylinder (6) are fixed in the space (11) that slides, the groove (12) that slides have been seted up in the space (11) that slides, guide cylinder (6) slide install in slide on groove (12).
3. The precision cell cutting device according to claim 2, wherein: be equipped with limiting plate (13) in slippage space (11), limiting plate (13) are including last limiting plate (131) and lower limiting plate (132), go up limiting plate (131) and all fix on knockout drum (1) with lower limiting plate (132).
4. The precision cell cutting device according to claim 3, wherein: a connecting plate (133) is arranged between the upper limiting plate (131) and the lower limiting plate (132).
5. The precision cell cutting device according to claim 1, wherein: the separating cylinder (1) deviates from one end of the first protective cover (2) and is provided with a second protective cover (8).
6. The precision cell cutting device according to claim 5, wherein: the second protective cover (8) is also provided with a water inlet (21) and a sampling port/liquid inlet (22).
7. The precision cell cutting device according to claim 1, wherein: and the driving end (71) is fixedly connected with a driving wheel (72).
8. The precision cell cutting device according to claim 7, wherein: the driving wheel (72) is in a circular truncated cone shape, and the peripheral side of the driving wheel (72) is provided with a strip edge (73) for increasing friction force.
9. A method for using a cell precision cutting device based on any one of 1-7 is characterized by comprising the following steps:
s1: cell tissue liquid to be cut is injected into the separation cylinder (1) through a water inlet (21);
s2: the cutting height of the combined type cutter (3) is adjusted by the movable guide cylinder (6);
s3: the separating cylinder (1) is shaken up and down to enable cell tissue fluid to impact and cut on the combined type cutter (3) so as to achieve the purpose of cutting;
s4: after a certain period of operation, a sample is taken from the sample/liquid inlet (22) and the result of the operation is checked.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201911061503.9A CN110919706A (en) | 2019-11-01 | 2019-11-01 | Cell precision cutting device and use method thereof |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201911061503.9A CN110919706A (en) | 2019-11-01 | 2019-11-01 | Cell precision cutting device and use method thereof |
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| Publication Number | Publication Date |
|---|---|
| CN110919706A true CN110919706A (en) | 2020-03-27 |
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| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN201911061503.9A Pending CN110919706A (en) | 2019-11-01 | 2019-11-01 | Cell precision cutting device and use method thereof |
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| Country | Link |
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| CN (1) | CN110919706A (en) |
Citations (10)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| EP0193883A2 (en) * | 1985-03-08 | 1986-09-10 | GRUPPO LEPETIT S.p.A. | Well forming apparatus |
| CN102899241A (en) * | 2011-07-26 | 2013-01-30 | 日本光电工业株式会社 | Cell isolation instrument |
| US9192939B2 (en) * | 2010-03-23 | 2015-11-24 | Lipogems International S.P.A. | Device and a method for preparing a tissue |
| EP2985586A1 (en) * | 2013-04-11 | 2016-02-17 | Syoryukensetsu Corporation | Gel plate segmenting and dispensing device and segmenting and dispensing method |
| CN205275573U (en) * | 2015-12-25 | 2016-06-01 | 江苏希瑞干细胞技术有限公司 | Navel cord cutting device |
| CN107107068A (en) * | 2014-12-15 | 2017-08-29 | 人类脑波责任有限公司 | The decomposer of biomaterial and corresponding manufacture method and the method for preparing cell suspending liquid and tissue micro-shifting plant |
| CN206580842U (en) * | 2017-01-13 | 2017-10-24 | 北京银丰鼎诚生物工程技术有限公司 | It is a kind of to be used for the shredding mechanism of fetal brain tissue before Primary culture of neural stem cells |
| CN109790504A (en) * | 2016-09-29 | 2019-05-21 | 高雄医学大学 | One kind dividing cellifugal device from tissue |
| CN110172397A (en) * | 2019-06-28 | 2019-08-27 | 山东锐进生物科技有限公司 | A kind of processing unit |
| CN110387320A (en) * | 2018-04-19 | 2019-10-29 | 杭州键一生物科技有限公司 | A kind of full-automatic primary cell separator |
-
2019
- 2019-11-01 CN CN201911061503.9A patent/CN110919706A/en active Pending
Patent Citations (10)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| EP0193883A2 (en) * | 1985-03-08 | 1986-09-10 | GRUPPO LEPETIT S.p.A. | Well forming apparatus |
| US9192939B2 (en) * | 2010-03-23 | 2015-11-24 | Lipogems International S.P.A. | Device and a method for preparing a tissue |
| CN102899241A (en) * | 2011-07-26 | 2013-01-30 | 日本光电工业株式会社 | Cell isolation instrument |
| EP2985586A1 (en) * | 2013-04-11 | 2016-02-17 | Syoryukensetsu Corporation | Gel plate segmenting and dispensing device and segmenting and dispensing method |
| CN107107068A (en) * | 2014-12-15 | 2017-08-29 | 人类脑波责任有限公司 | The decomposer of biomaterial and corresponding manufacture method and the method for preparing cell suspending liquid and tissue micro-shifting plant |
| CN205275573U (en) * | 2015-12-25 | 2016-06-01 | 江苏希瑞干细胞技术有限公司 | Navel cord cutting device |
| CN109790504A (en) * | 2016-09-29 | 2019-05-21 | 高雄医学大学 | One kind dividing cellifugal device from tissue |
| CN206580842U (en) * | 2017-01-13 | 2017-10-24 | 北京银丰鼎诚生物工程技术有限公司 | It is a kind of to be used for the shredding mechanism of fetal brain tissue before Primary culture of neural stem cells |
| CN110387320A (en) * | 2018-04-19 | 2019-10-29 | 杭州键一生物科技有限公司 | A kind of full-automatic primary cell separator |
| CN110172397A (en) * | 2019-06-28 | 2019-08-27 | 山东锐进生物科技有限公司 | A kind of processing unit |
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Application publication date: 20200327 |