CN110917286A - Antimicrobial traditional Chinese medicine composition, and preparation method and application of antimicrobial preparation - Google Patents
Antimicrobial traditional Chinese medicine composition, and preparation method and application of antimicrobial preparation Download PDFInfo
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Abstract
The invention discloses an antimicrobial traditional Chinese medicine composition, and a preparation method and application of an antimicrobial preparation. The raw materials of the antimicrobial traditional Chinese medicine composition comprise main materials, namely phellodendron, humifuse euphorbia herb, ash bark, myrobalan, aloe, forsythia fruit oil, dandelion and purslane, which are nontoxic and harmless components to animals, can avoid or reduce the side effect of the organisms after the use of the composition, and the main components can be mutually cooperated, especially after the aloe and the dandelion are added, the antibacterial and disinfection effects of the traditional Chinese medicine composition can be obviously enhanced, and the growth of harmful microorganisms can be effectively inhibited; meanwhile, the components of the traditional Chinese medicine composition are easy to degrade in the environment, and the traditional Chinese medicine composition has the advantages of environmental protection, low preparation cost and suitability for application and popularization.
Description
Technical Field
The invention relates to the technical field of antimicrobial preparations, in particular to an antimicrobial traditional Chinese medicine composition, and a preparation method and application of an antimicrobial preparation.
Background
The pathogens grow and reproduce in the animal body, release toxic substances and the like to cause pathological changes of the body to different degrees, and the process is called infection. The problems of infection and septicemia caused by harmful microorganisms invaded in the body are not inconstant, but the skin is red and swollen slightly, and the healthy life is harmed seriously. When the body surface of a machine body is invaded by harmful microorganisms, the disinfection and sterilization of the body surface are particularly important.
The existing microbial disinfectants can be divided into high-efficiency disinfectants, medium-efficiency disinfectants and low-efficiency disinfectants according to the action level. The high-efficiency disinfectant and the middle-efficiency disinfectant generally have strong oxidizing property or strong reducing property and good disinfection and sterilization effects, but on the other hand, the high-efficiency disinfectant and the middle-efficiency disinfectant also have strong corrosivity, irritation and toxicity and can cause certain damage to animals, for example, when the o-phthalaldehyde is contacted with the skin, the skin can be burnt into black, when the peracetic acid and the like are used, the skin can be burnt into white and unbearable, when the chlorine-containing disinfectant is used, the respiratory tract of the animals can be strongly stimulated, and the phenol disinfectant can be absorbed by the skin and damaged wounds of the animals to generate strong toxicity to damage the nervous systems of the animals and the like.
The low-efficiency disinfectant, such as quaternary ammonium salt and biguanide disinfectant, has the advantages of less corrosivity to objects, less pollution to the environment, less irritation and toxicity to animals, but has low sterilizing capability, and is difficult to meet corresponding requirements in most cases.
In order to replace unfavorable components in the disinfectant, effective sterilization and disinfection can be guaranteed, meanwhile, no pollution is caused to the environment, the advantages of no residue, no side effect, no harm to the environment and the like of the traditional Chinese medicines are gradually taken into consideration, and a trend is gradually developed for how to reasonably select the traditional Chinese medicine composition to prepare the disinfectant and the disinfectant.
In view of this, the invention is particularly proposed.
Disclosure of Invention
The invention aims to provide an antimicrobial traditional Chinese medicine composition and a preparation method and application of an antimicrobial preparation, wherein the components of the antimicrobial traditional Chinese medicine composition are mutually cooperated to achieve the remarkable antibacterial and disinfection effects, and the components of the traditional Chinese medicine composition are all harmless components to animals, so that the side effects of the organisms after use can be avoided or reduced.
The invention is realized by the following steps:
in a first aspect, an embodiment of the present invention provides an antimicrobial traditional Chinese medicine composition, which comprises raw materials including, by weight: 13-20 parts of golden cypress, 8-18 parts of humifuse euphorbia herb, 6-12 parts of cortex fraxini, 3-11 parts of myrobalan, 14-22 parts of aloe, 6-14 parts of forsythia suspensa oil, 10-17 parts of dandelion and 12-19 parts of purslane.
The traditional Chinese medicine composition adopts components which are non-toxic and harmless to animals, and the components achieve remarkable antibacterial and disinfection effects through mutual synergistic action. In addition, the traditional Chinese medicine composition can avoid or reduce the side effect of the organism after use, is easy to degrade in the environment, is green and environment-friendly, has low preparation cost, and is suitable for application and popularization.
In an optional embodiment, the main material comprises the following components in parts by weight: 15-18 parts of golden cypress, 10-16 parts of humifuse euphorbia herb, 8-10 parts of cortex fraxini, 5-9 parts of myrobalan, 16-20 parts of aloe, 8-12 parts of forsythia suspensa oil, 12-15 parts of dandelion and 14-17 parts of purslane. The antibacterial and disinfection effects of the composition can be further improved according to the proportion.
In an alternative embodiment, the raw material further comprises an auxiliary material, and the auxiliary material comprises at least one of acacia, tween 80, glyceryl monostearate, glycerol, phenethanol, triethanolamine, and lactic acid. The auxiliary materials adopted by the embodiment of the application are substances which are small in irritation to organisms and free of toxic and side effects, and can further synergistically enhance the sterilization and bacteriostasis effects of the main materials and effectively inhibit the growth of microorganisms.
In an optional embodiment, according to the weight part standard of the main material, the auxiliary material comprises the following components: 20 to 30 parts of Arabic gum, 15 to 18 parts of Tween 80, 25 to 38 parts of glyceryl monostearate, 50 to 60 parts of glycerol, 2 to 5 parts of phenethyl alcohol, 12 to 20 parts of triethanolamine and 15 to 20 parts of lactic acid.
In an optional embodiment, the main materials include phellodendron amurense, humifuse euphorbia herb, ash bark of ash, myrobalan and purslane, and the main materials are obtained by cleaning, drying and crushing, and the crushed particle size is 24-50 meshes.
In a second aspect, the present invention provides a method for preparing an antimicrobial preparation, which comprises mixing the components of the antimicrobial Chinese medicinal composition according to any one of the preceding embodiments.
In an alternative embodiment, before the mixing, the preparation method comprises extracting raw materials of the antimicrobial agent to obtain an aloe extract, a dandelion extract and a mixed solution of phellodendron, humifuse euphorbia herb, ash bark, myrobalan and purslane.
In an optional embodiment, the mixing of the components of the traditional Chinese medicine composition comprises mixing the mixed solution, the aloe extract, the dandelion extract and the forsythia oil according to parts by weight, and heating to form an extract.
In an optional embodiment, the density of the extract is 1.1-1.2 g/cm3。
In an alternative embodiment, the aloe extract, the dandelion extract and the mixture are extracted by hot reflux extraction.
In an alternative embodiment, the extraction conditions of the aloe vera extract are: mixing an aloe raw material with water which is 8-10 times of the weight of the aloe raw material, heating and refluxing for extraction at the extraction temperature of 60-80 ℃ for 1.5-3 h for 2-3 times, filtering, combining filtrates, and concentrating to obtain an aloe extract.
In an alternative embodiment, the conditions for extracting the dandelion extract are as follows: cutting and cleaning dandelion raw materials, soaking, heating and refluxing for extraction at the extraction temperature of 60-80 ℃ for 1.5-2 h, standing, filtering and concentrating to obtain a dandelion extract.
In an alternative embodiment, the extraction conditions of the mixed solution are: mixing a mixture of phellodendron amurense, humifuse euphorbia herb, ash bark, myrobalan and purslane with water which is 8-10 times of the weight of the mixture, and carrying out heating reflux extraction at the extraction temperature of 95-100 ℃ for 1.5-2 h for 2-3 times.
In an optional embodiment, the thermal reflux extraction of the mixed solution is enzymolysis-assisted thermal reflux extraction:
the enzymolysis conditions of the mixture are as follows: carrying out enzymolysis on the raw materials of the mixed solution by adopting cellulase, wherein the dosage of the cellulase is 0.5-1.0 percent of the mass of the mixed solution after mixing the phellodendron, the humifuse euphorbia herb, the ash bark, the myrobalam and the purslane, the pH value is 4.0-5.0, the enzymolysis time is 2-3 h, and the enzymolysis temperature is 35-45 ℃;
in an alternative embodiment, when the raw materials for the antimicrobial agent include adjuvants, the method of making includes sieving a melt of the adjuvants and mixing with the extract to produce the antimicrobial agent.
In an alternative embodiment, the melting temperature of the adjuvant is 70 ℃ to 90 ℃.
In an optional embodiment, the sieving is to sieve the mixture by 80-120 meshes; preferably through a 100 mesh screen.
In a third aspect, the present invention also provides an antimicrobial composition as defined in any one of the preceding embodiments or an antimicrobial agent prepared by the method of preparing an antimicrobial agent as defined in any one of the preceding embodiments for use in disinfecting, anti-inflammatory and/or cleaning an animal body.
The invention has the following beneficial effects:
the embodiment of the invention provides an antimicrobial traditional Chinese medicine composition and a preparation method and application thereof, the raw materials of the antimicrobial traditional Chinese medicine composition comprise main materials, namely phellodendron, humifuse euphorbia herb, ash bark, myrobalan, aloe, forsythia oil, dandelion and purslane, which are all nontoxic and harmless components to animals, so that the side effects of the organisms after the use of the antimicrobial traditional Chinese medicine composition can be avoided or reduced, the main components can be mutually cooperated, especially the antibacterial and disinfection effects of the traditional Chinese medicine composition can be obviously enhanced after the aloe and the dandelion are added, and the growth of harmful microorganisms can be effectively inhibited; meanwhile, the components of the traditional Chinese medicine composition are easy to degrade in the environment, and the traditional Chinese medicine composition has the advantages of environmental protection, low preparation cost and suitability for application and popularization.
Detailed Description
In order to make the objects, technical solutions and advantages of the embodiments of the present invention clearer, the technical solutions in the embodiments of the present invention will be clearly and completely described below. The examples, in which specific conditions are not specified, were conducted under conventional conditions or conditions recommended by the manufacturer. The reagents or instruments used are not indicated by the manufacturer, and are all conventional products available commercially.
The features and properties of the present invention are described in further detail below with reference to examples.
Example 1
The embodiment provides an antimicrobial traditional Chinese medicine composition and a preparation method of an antimicrobial preparation.
An antimicrobial Chinese medicinal composition comprises main materials and adjuvants.
The main materials comprise the following components in parts by weight: 15 parts of phellodendron amurense, 10 parts of humifuse euphorbia herb, 8 parts of ash bark, 5 parts of myrobalan, 16 parts of aloe, 8 parts of forsythia oil, 12 parts of dandelion and 14 parts of purslane.
The auxiliary materials comprise the following components in parts by weight: 20 parts of Arabic gum, 15 parts of Tween 80, 25 parts of glyceryl monostearate, 50 parts of glycerol, 2 parts of phenethyl ester, 12 parts of triethanolamine and 15 parts of lactic acid.
The preparation method of the antimicrobial preparation comprises the following steps of preparing the components of the antimicrobial traditional Chinese medicine composition:
(1) mixing liquid:
pulverizing cleaned and dried cortex Phellodendri, herba Euphorbiae Humifusae, cortex Fraxini, fructus Chebulae and herba Portulacae to 24 mesh; mixing the phellodendron, the humifuse euphorbia herb, the ash bark, the myrobalan and the purslane according to the weight ratio, and performing enzymolysis-assisted thermal reflux extraction on the mixture.
The enzymolysis conditions are as follows: the dosage of the cellulase is 0.5 percent of the mass of the mixture, the pH is 4.0, the enzymolysis time is 2 hours, and the enzymolysis temperature is 35 ℃;
the extraction conditions are as follows: extracting with 8 times of water at 95 deg.C for 1.5 hr for 2 times.
Standing the extract for 24h after enzymolysis extraction, filtering, and concentrating the filtrate to obtain a mixed solution;
(2) aloe extract liquid: mixing aloe with water 8 times of the weight of aloe, and extracting by a hot reflux extraction method under the following extraction conditions: extracting at 60 deg.C for 2 times for 1.5 hr, standing for 24 hr, filtering (with filter hole of 100 μm, in other embodiments, 80-120 μm), filtering, and concentrating the filtrate to obtain aloe extractive solution;
(3) dandelion extract: cleaning herba Taraxaci, chopping, soaking, and extracting by hot reflux extraction at 60 deg.C for 1.5 hr. Standing for 24h, filtering (with a filter hole of 100 μm, in other embodiments, selected in the range of 80-120 μm), and concentrating to obtain herba Taraxaci extractive solution;
(4) mixing the mixed solution, the aloe extract and the dandelion extract prepared in the steps (1) - (3) with the forsythia oil according to the weight parts, heating to 100 ℃, and evaporating water until the density reaches 1.10g/cm3To form an extract;
(5) mixing the above adjuvants in parts by weight, heating to 80 deg.C to melt the adjuvants, filtering with 100 mesh screen to obtain adjuvant melt, and mixing the melt and the extract to obtain antimicrobial agent.
Example 2
The present example provides an antimicrobial Chinese medicinal composition and a method for preparing an antimicrobial preparation, which are substantially the same as those in example 1, except for the differences in parameters, as follows.
An antimicrobial traditional Chinese medicine composition comprises main materials and auxiliary materials:
the main materials comprise the following components in parts by weight: 18 parts of phellodendron amurense, 16 parts of humifuse euphorbia herb, 10 parts of ash bark, 9 parts of myrobalan, 20 parts of aloe, 12 parts of forsythia oil, 15 parts of dandelion and 17 parts of purslane.
The auxiliary materials comprise the following components in parts by weight: 30 parts of Arabic gum, 18 parts of Tween 80, 38 parts of glyceryl monostearate, 60 parts of glycerol, 5 parts of phenethyl ester, 20 parts of triethanolamine and 20 parts of lactic acid.
The preparation method of the antimicrobial preparation comprises the following steps of preparing the components of the antimicrobial traditional Chinese medicine composition:
(1) mixing liquid:
pulverizing cleaned and dried cortex Phellodendri, herba Euphorbiae Humifusae, cortex Fraxini, fructus Chebulae and herba Portulacae to 50 mesh; mixing the phellodendron, the humifuse euphorbia herb, the ash bark, the myrobalan and the purslane according to the weight ratio, and performing enzymolysis-assisted thermal reflux extraction on the mixture.
The enzymolysis conditions are as follows: the dosage of the cellulase is 1.0 percent of the mass of the mixture, the pH is 5.0, the enzymolysis time is 3 hours, and the enzymolysis temperature is 45 ℃;
the extraction conditions are as follows: the mixture is 10 times of water (weight), the extraction temperature is 100 ℃, the extraction time is 2h, and the extraction times are 3 times.
After enzymolysis extraction, filtering the extracting solution, taking filtrate and concentrating to obtain mixed solution;
(2) aloe extract liquid: mixing aloe with water, wherein the weight of the water is 10 times of that of the aloe, and extracting by adopting a hot reflux extraction method, wherein the extraction conditions are as follows: extracting at 80 deg.C for 3 times for 3 hr, filtering, and concentrating the filtrate to obtain aloe extractive solution;
(3) dandelion extract: cleaning herba Taraxaci, chopping, soaking, extracting with hot reflux extraction at 80 deg.C for 2 hr, standing for 36 hr, filtering (filter hole of 100 μm), and concentrating to obtain herba Taraxaci extractive solution;
(4) mixing the mixed solution, Aloe extractive solution, herba Taraxaci extractive solution and oleum forsythiae prepared in steps (1) - (3), heating to 100 deg.C, evaporating water until the mixture is denseThe degree reaches 1.20g/cm3To form an extract;
(5) mixing the above adjuvants according to weight parts, heating to 80 deg.C, melting to obtain adjuvant melt, filtering with 100 mesh screen, and mixing the melt with the extract to obtain antimicrobial agent.
Example 3
The present embodiment provides a method for preparing a Chinese medicinal composition and an antimicrobial agent, which is substantially the same as embodiments 1-2, and differs from the embodiments in the following parameters.
An antimicrobial traditional Chinese medicine composition comprises main materials and auxiliary materials:
the main materials comprise the following components in parts by weight: 17 parts of phellodendron amurense, 13 parts of humifuse euphorbia herb, 9 parts of ash bark, 7 parts of myrobalan, 18 parts of aloe, 10 parts of forsythia oil, 13 parts of dandelion and 15 parts of purslane.
The auxiliary materials comprise the following components in parts by weight: 25 parts of Arabic gum, 17 parts of Tween 80, 30 parts of glyceryl monostearate, 55 parts of glycerol, 4 parts of phenethyl ester, 16 parts of triethanolamine and 17 parts of lactic acid.
The preparation method of the antimicrobial preparation comprises the following steps of preparing the components of the antimicrobial traditional Chinese medicine composition:
(1) mixing liquid: pulverizing cleaned and dried cortex Phellodendri, herba Euphorbiae Humifusae, cortex Fraxini, fructus Chebulae and herba Portulacae to 50 mesh; mixing the phellodendron, the humifuse euphorbia herb, the ash bark, the myrobalan and the purslane according to the weight ratio, and performing enzymolysis-assisted thermal reflux extraction on the mixture.
The enzymolysis conditions are as follows: the dosage of the cellulase is 0.8 percent of the mass of the mixture, the pH is 4.5, the enzymolysis time is 2.5h, and the enzymolysis temperature is 40 ℃;
the extraction conditions are as follows: extracting with 9 times of water at 98 deg.C for 1.8 hr for 3 times.
Filtering the extract after enzymolysis extraction, and concentrating the filtrate to obtain a mixed solution;
(2) aloe extract liquid: mixing aloe with water 9 times of the weight of aloe, and extracting by adopting a hot reflux extraction method under the following extraction conditions: extracting at 70 deg.C for 3 times for 2.5 hr, filtering, and concentrating the filtrate to obtain aloe extractive solution;
(3) dandelion extract: cleaning herba Taraxaci, chopping, soaking, and extracting by hot reflux extraction at 70 deg.C for 1.8 hr. After extraction, standing for 30 hours, filtering (the filter hole is 80-120 mu m), and concentrating to obtain a dandelion extract;
(4) mixing the mixed solution, Aloe extractive solution, herba Taraxaci extractive solution and oleum forsythiae prepared in steps (1) - (3), heating to 100 deg.C, evaporating water until the density reaches 1.15g/cm3To form an extract;
(5) mixing the above adjuvants according to weight parts, heating to 80 deg.C, melting to obtain adjuvant melt, filtering with 100 mesh screen, and mixing with the extract to obtain antimicrobial agent.
Example 4
This example provides a method for preparing a herbal composition and an antimicrobial agent, which is substantially the same as example 1, except that the herbal composition and the antimicrobial agent are prepared without adding phenylethanol in an adjuvant.
Example 5
This example provides a method for preparing a herbal composition and an antimicrobial agent, which is substantially the same as example 1, except that the herbal composition and the antimicrobial agent are prepared without adding triethanolamine as an auxiliary material.
Example 6
This example provides a method for preparing a herbal composition and an antimicrobial agent, which is substantially the same as example 1, except that no lactic acid is added to the adjuvant.
Comparative example 1
This example provides a method for preparing a Chinese medicinal composition and an antimicrobial agent, which is substantially the same as example 1, except that the aloe/aloe extract solution in the method for preparing a Chinese medicinal composition and an antimicrobial agent is replaced with the same amount of dandelion/dandelion extract solution.
Comparative example 2
This example provides a method for preparing a Chinese medicinal composition and an antimicrobial agent, which is substantially the same as example 1, except that the method for preparing a Chinese medicinal composition and an antimicrobial agent comprises replacing the same amount of aloe/aloe extract with the dandelion/dandelion extract.
Comparative example 3
This example provides a method for preparing a Chinese medicinal composition and an antimicrobial agent, which is substantially the same as example 1 except that the forsythia suspense oil in the method for preparing a Chinese medicinal composition and an antimicrobial agent is replaced by the aloe extract in the same weight parts.
Verification example 1
The antimicrobial properties of the antimicrobial formulations provided in examples 1-6 were verified.
Verification method
(a) Representative laboratory species, staphylococcus aureus, candida albicans, escherichia coli, and pseudomonas aeruginosa, were selected. Respectively inoculating the four strains on four agar culture media, culturing at 37 ℃ for 24 hours, respectively selecting typical colonies from the agar culture media, respectively inoculating the typical colonies into four fresh nutrient broth culture media, and culturing at the same temperature for 24 hours for later use;
(b) taking 10ml of each of 6 groups of antimicrobial preparations prepared in examples 1-6, comparative examples 1-3 and comparative example 4 (iodophor) of the invention, respectively placing the preparations in dilution containers, respectively diluting the preparations by 25 times, respectively placing the dilutions in the containers, and placing the containers under aseptic conditions for later use;
(c) respectively diluting the experimental strains cultured in the nutrient broth culture medium for 24 hours in the step (a) by 50 times by using sterile nutrient broth, and respectively packaging each strain into 4 culture dishes, wherein examples 1 to 6 and comparative examples 1 to 4 are respectively marked;
the diluted solutions obtained in examples 1 to 6 of the step (b) were dropped into the petri dishes of the experimental group, and the diluted solutions of comparative examples 1 to 3 were dropped into the petri dishes of the control group. The residual area of the colony was measured, and the results are shown in Table 1.
TABLE 1 test results
As can be seen from Table 1, compared with comparative examples 1 to 4, the external antimicrobial traditional Chinese medicine composition preparation provided by embodiments 1 to 6 of the invention has significantly better effect on resisting Staphylococcus aureus, Candida albicans, Escherichia coli and Pseudomonas aeruginosa than comparative examples 1 to 4;
according to the results of comparative examples 1-2 and examples 1-6, the dandelion extract and the aloe extract have synergistic effect, supplement each other, and can significantly improve the antimicrobial capability of the traditional Chinese medicine composition preparation when added into the traditional Chinese medicine composition;
according to the comparison between the results of the comparative example 3 and the results of the examples 1 to 6, the forsythia suspense oil has a remarkable influence on the antimicrobial capability of the traditional Chinese medicine composition preparation;
in the examples of the present application, the external antimicrobial traditional Chinese medicine composition preparation provided in example 1 has the best ability to inhibit various bacterial species, and the effect against staphylococcus aureus, candida albicans, escherichia coli and pseudomonas aeruginosa is significantly better than that of other embodiments.
In conclusion, the invention provides a traditional Chinese medicine composition, the raw materials of the traditional Chinese medicine composition comprise main materials, the main materials comprise phellodendron, humifuse euphorbia herb, ash bark, myrobalan, aloe, forsythia oil, dandelion and purslane, the main materials are nontoxic and harmless components to animals, the side effects of the organisms after the traditional Chinese medicine composition is used can be avoided or reduced, the main components can be mutually cooperated, especially the antibacterial and disinfection effects of the traditional Chinese medicine composition can be obviously enhanced after the aloe and the dandelion are added, and the growth of harmful microorganisms can be effectively inhibited; meanwhile, the components of the traditional Chinese medicine composition are easy to degrade in the environment, and the traditional Chinese medicine composition has the advantages of environmental protection, low preparation cost and suitability for application and popularization.
In addition, the application also provides an antimicrobial preparation prepared by adopting the traditional Chinese medicine composition, a preparation method of the antimicrobial preparation, the antimicrobial preparation prepared by the preparation method of the antimicrobial preparation and application of the traditional Chinese medicine composition in preparing the antimicrobial preparation. The antimicrobial agent has an effect of being easily prepared and remarkably inhibiting the growth of microorganisms.
The above description is only a preferred embodiment of the present invention and is not intended to limit the present invention, and various modifications and changes may be made by those skilled in the art. Any modification, equivalent replacement, or improvement made within the spirit and principle of the present invention should be included in the protection scope of the present invention.
Claims (10)
1. The antimicrobial traditional Chinese medicine composition is characterized by comprising the following main materials in parts by weight: 13-20 parts of golden cypress, 8-18 parts of humifuse euphorbia herb, 6-12 parts of cortex fraxini, 3-11 parts of myrobalan, 14-22 parts of aloe, 6-14 parts of forsythia suspensa oil, 10-17 parts of dandelion and 12-19 parts of purslane.
2. The antimicrobial traditional Chinese medicine composition according to claim 1, wherein the main material comprises the following components in parts by weight: 15-18 parts of golden cypress, 10-16 parts of humifuse euphorbia herb, 8-10 parts of cortex fraxini, 5-9 parts of myrobalan, 16-20 parts of aloe, 8-12 parts of forsythia suspensa oil, 12-15 parts of dandelion and 14-17 parts of purslane.
3. The antimicrobial Chinese medicinal composition according to claim 2, wherein the raw materials further comprise an auxiliary material, and the auxiliary material comprises at least one of acacia, tween 80, glycerol monostearate, glycerol, phenethanol, triethanolamine, and lactic acid;
preferably, according to the weight part standard of the main material, the auxiliary material comprises the following components: 20 to 30 parts of Arabic gum, 15 to 18 parts of Tween 80, 25 to 38 parts of glyceryl monostearate, 50 to 60 parts of glycerol, 2 to 5 parts of phenethyl alcohol, 12 to 20 parts of triethanolamine and 15 to 20 parts of lactic acid.
4. The antimicrobial traditional Chinese medicine composition according to claim 1, wherein the main materials comprise phellodendron amurense, humifuse euphorbia herb, ash bark of ash, myrobalan and purslane, and the main materials are obtained by cleaning, drying and crushing the raw materials, wherein the crushed particle size is 24-50 meshes.
5. A method for preparing an antimicrobial preparation, comprising mixing the components of the antimicrobial Chinese medicinal composition according to any one of claims 1 to 4;
preferably, before mixing, the preparation method comprises extracting raw materials of the antimicrobial preparation to obtain aloe extract, dandelion extract and a mixed solution of cortex Phellodendri, herba Euphorbiae Humifusae, cortex Fraxini, fructus Chebulae and herba Portulacae;
preferably, the mixing of the components of the traditional Chinese medicine composition comprises: mixing the mixed solution, the aloe extract, the dandelion extract and the forsythia oil according to parts by weight, and heating to form an extract;
preferably, the density of the extract is 1.1-1.2 g/cm3。
6. The method for preparing an antimicrobial agent according to claim 5, wherein the aloe extract, the dandelion extract and the mixture are extracted by thermal reflux extraction;
preferably, the extraction conditions of the aloe extract are as follows: mixing an aloe raw material with water which is 8-10 times of the weight of the aloe raw material, heating, refluxing and extracting at the extraction temperature of 60-80 ℃ for 1.5-3 hours for 2-3 times, filtering, combining filtrates, and concentrating to obtain an aloe extracting solution;
preferably, the extraction conditions of the dandelion extract are as follows: cutting and cleaning dandelion raw materials, soaking, heating and refluxing for extraction at the extraction temperature of 60-80 ℃ for 1.5-2 h, standing, filtering and concentrating to obtain a dandelion extract;
preferably, the mixed solution extraction conditions are as follows: mixing a mixture of phellodendron amurense, humifuse euphorbia herb, ash bark, myrobalan and purslane with water which is 8-10 times of the weight of the mixture, and carrying out heating reflux extraction at the extraction temperature of 95-100 ℃ for 1.5-2 h for 2-3 times.
7. The method of claim 6, wherein the thermal reflux extraction of the mixed solution is an enzymatic assisted thermal reflux extraction:
the enzymolysis conditions of the mixture are as follows: carrying out enzymolysis on the raw materials of the mixed solution by adopting cellulase, wherein the dosage of the cellulase is 0.5-1.0 percent of the mass of the mixed solution after mixing the phellodendron, the humifuse euphorbia herb, the ash bark, the myrobalam and the purslane, the pH value is 4.0-5.0, the enzymolysis time is 2-3 h, and the enzymolysis temperature is 35-45 ℃.
8. The method for preparing an antimicrobial agent according to any one of claims 5 to 7, wherein when the raw materials of the antimicrobial agent include an auxiliary material, the method comprises sieving a melt of the auxiliary material and mixing the sieved material with the extract to obtain the antimicrobial agent.
9. The method of claim 8, wherein the adjuvant has a melting temperature of 70 to 90 ℃;
preferably, the sieving is 80-120 mesh sieving.
10. Use of an antimicrobial combination according to any one of claims 1 to 4 or an antimicrobial preparation prepared by a method of preparing an antimicrobial preparation according to any one of claims 5 to 9 for disinfecting, sterilizing, anti-inflammatory or cleaning an animal body.
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