CN110903958A - A breaker for centrifugation draws histiocyte - Google Patents
A breaker for centrifugation draws histiocyte Download PDFInfo
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- CN110903958A CN110903958A CN201911247900.5A CN201911247900A CN110903958A CN 110903958 A CN110903958 A CN 110903958A CN 201911247900 A CN201911247900 A CN 201911247900A CN 110903958 A CN110903958 A CN 110903958A
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- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
- C12M45/00—Means for pre-treatment of biological substances
- C12M45/02—Means for pre-treatment of biological substances by mechanical forces; Stirring; Trituration; Comminuting
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Abstract
The invention discloses a crushing device for centrifugally extracting histiocytes, which comprises a containing barrel, wherein the containing barrel comprises a containing barrel main body, a first rotating hole is arranged on the containing barrel main body, the first rotating hole is communicated with the containing barrel main body, a motor supporting piece is arranged on the containing barrel main body, and a second rotating hole is arranged on the motor supporting piece. According to the crushing device, quartz sand is put into the sand inlet hopper, the first motor drives the first stirring piece to rotate, meanwhile, the second stirring piece also rotates, the rotation directions of the first stirring piece and the second stirring piece in the radial direction are the same, the second spiral blade effectively prevents the quartz sand from settling at the bottom of the containing cylinder main body, the rotation directions of the first spiral blade and the third spiral blade are opposite, so that the stirring directions of histiocytes in the axial direction are opposite, the histiocytes are fully contacted with the quartz sand, and the cell crushing efficiency is improved; the ultrasonic oscillator is controlled to move up and down through the forward and reverse rotation of the second motor, and the efficiency of oscillation and crushing is improved.
Description
Technical Field
The invention relates to a crushing device, in particular to a crushing device for centrifugally extracting histiocytes.
Background
Among the traditional cell disruption device, place hydraulic pressure station with broken high-pressure part front and back, wherein hydraulic pressure station includes oil tank and oil pump, and area is great, not only is unfavorable for storing, need bypass hydraulic pressure station during the broken high-pressure part of personnel operation moreover, and it is extremely inconvenient to operate. In addition, at present, air cooling is adopted for heat exchange of hydraulic oil, the efficiency is low, a heat exchanger with a large volume is required for achieving sufficient heat exchange amount of the hydraulic oil, the overall quality and the cost of the cell disruption device are increased, and the efficiency is low.
Disclosure of Invention
The invention aims to provide a crushing device for centrifugally extracting histiocytes, the histiocytes are conveyed into a containing cylinder main body through a feed pipe, quartz sand is put into the containing cylinder main body through a sand inlet hopper, a first motor drives a first stirring piece to rotate, meanwhile, a second stirring piece also rotates along with the first stirring piece, the first stirring piece and the second stirring piece rotate in the same direction in the radial direction, a second helical blade effectively prevents the quartz sand from settling at the bottom of the containing cylinder main body, and the blade rotating directions of the first helical blade and a third helical blade are opposite, so that the stirring directions of the histiocytes in the axial direction are opposite, the histiocytes are fully contacted with the quartz sand, and the cell crushing efficiency is improved; the ultrasonic oscillator is controlled to move up and down by the forward and reverse rotation of the second motor, and the efficiency of vibration and crushing is improved.
The purpose of the invention can be realized by the following technical scheme:
the utility model provides a breaker for centrifugation draws histiocyte, breaker includes a splendid attire section of thick bamboo, a splendid attire section of thick bamboo is equipped with first rotation hole including a splendid attire section of thick bamboo main part in the splendid attire section of thick bamboo main part, first rotation hole and a splendid attire section of thick bamboo main part intercommunication, are equipped with motor support piece in the splendid attire section of thick bamboo main part, and the last second that is equipped with of motor support piece rotates the hole, is equipped with.
The sand feeding device is characterized in that third through holes are distributed in the containing barrel main body in an array mode by taking the first rotating holes as centers, a sand feeding hopper is arranged on the containing barrel main body, a feeding pipe is arranged on the containing barrel main body, a first control valve is connected onto the feeding pipe, a discharging pipe is arranged below the containing barrel main body, and a second control valve is arranged on the discharging pipe.
One side of the containing cylinder main body is provided with first supporting plates which are symmetrically distributed, and a mounting plate is arranged between the first supporting plates.
Be equipped with first motor on the splendid attire section of thick bamboo, the output shaft of first motor passes the second and rotates hole fastening connection and have first stirring piece, and first stirring piece includes first rotation pole, is equipped with first helical blade and second helical blade on the first rotation pole, is equipped with first belt pulley on the first rotation pole.
The second stirring piece that the splendid attire section of thick bamboo internal rotation was equipped with the array and distributes, and the second stirring piece includes the second dwang, is equipped with third helical blade on the second dwang, is equipped with the second belt pulley on the second dwang.
The first belt pulley and the second belt pulley are double-groove belts, a first belt is arranged on the first belt pulley, and a second belt is arranged on the second belt pulley.
The improved multifunctional bottle cap is characterized in that a sliding support piece is arranged on the containing barrel, a second motor is arranged on the sliding support piece, the sliding support piece comprises symmetrically distributed slide rail supporting blocks, slide rails are arranged on the slide rail supporting blocks, stop blocks are arranged at two ends of each slide rail supporting block in a conveying mode, lead screws are arranged between the stop blocks, a detection support piece is arranged on each slide rail supporting block and comprises a second supporting plate and a third supporting plate which are perpendicular to each other, a connecting block is arranged on the second supporting plate, and.
Further, the second rotating hole and the first rotating hole are coaxial.
Further, the first helical blade and the second helical blade have the same helical rotation direction.
Further, the first stirring piece is positioned in the first rotating hole through the first rotating rod and rotates.
Furthermore, the blade rotating direction of the third helical blade is opposite to that of the first helical blade, and the second stirring piece is positioned in the third through hole through the second rotating rod and rotates.
Further, the length of the second rotating rod is smaller than that of the first rotating rod.
Furthermore, the first belt pulley is rotatably connected with any one second belt pulley through a first belt.
Furthermore, two adjacent second belt wheels are rotatably connected through a second belt, wherein the second belt wheel rotatably connected with the first belt is only rotatably connected with the second belt wheel adjacent to one side through the second belt.
Further, the sliding support piece is matched with the screw rod through a threaded hole.
Furthermore, the second supporting plate is located on the sliding rails and slides, the connecting blocks are located between the sliding rails which are symmetrically distributed, the output shaft of the second motor is rotatably connected with the lead screw, and the third supporting plate is provided with an ultrasonic oscillator.
The invention has the beneficial effects that:
1. according to the crushing device, quartz sand is put into the sand inlet hopper, the first motor drives the first stirring piece to rotate, meanwhile, the second stirring piece also rotates, the rotation directions of the first stirring piece and the second stirring piece in the radial direction are the same, the second spiral blade effectively prevents the quartz sand from settling at the bottom of the containing cylinder main body, the rotation directions of the first spiral blade and the third spiral blade are opposite, so that the stirring directions of histiocytes in the axial direction are opposite, the histiocytes are fully contacted with the quartz sand, and the cell crushing efficiency is improved;
2. the crushing device controls the ultrasonic oscillator to move up and down through the forward and reverse rotation of the second motor, so that the efficiency of vibration crushing is improved.
Drawings
The invention will be further described with reference to the accompanying drawings.
FIG. 1 is a schematic view of the crushing apparatus of the present invention;
FIG. 2 is a schematic view of the cartridge of the present invention;
FIG. 3 is a schematic view of a portion of the crushing apparatus of the present invention;
FIG. 4 is a schematic view of a first stirring member according to the present invention;
FIG. 5 is a schematic view of a second stirring member according to the present invention;
fig. 6 is a schematic view of a part of the structure of the crushing device of the invention.
Detailed Description
The technical solutions in the embodiments of the present invention will be clearly and completely described below with reference to the drawings in the embodiments of the present invention, and it is obvious that the described embodiments are only a part of the embodiments of the present invention, and not all of the embodiments. All other embodiments, which can be derived by a person skilled in the art from the embodiments given herein without making any creative effort, shall fall within the protection scope of the present invention.
The utility model provides a breaker for centrifugation draws histiocyte, breaker includes a splendid attire section of thick bamboo 1, as shown in fig. 1, fig. 2, a splendid attire section of thick bamboo 1 includes a splendid attire section of thick bamboo main part 11, is equipped with first rotation hole 12 on the splendid attire section of thick bamboo main part 11, and first rotation hole 12 and a splendid attire section of thick bamboo main part 11 intercommunication are equipped with motor support piece 13 on the splendid attire section of thick bamboo main part 11, and motor support piece 13 is last to be equipped with the second and to rotate hole 132, and motor support piece 13 is last to be equipped with.
The third through holes 14 are distributed on the containing barrel main body 11 in an array mode by taking the first rotating holes 12 as centers, a sand inlet hopper 15 is arranged on the containing barrel main body 11, a feeding pipe 16 is arranged on the containing barrel main body 11, the feeding pipe 16 is connected with a first control valve 10, a discharging pipe 17 is arranged below the containing barrel main body 11, and a second control valve 20 is arranged on the discharging pipe 17.
One side of the containing cylinder body 11 is provided with first supporting plates 18 which are symmetrically distributed, and a mounting plate 19 is arranged between the first supporting plates 18.
Be equipped with first motor 2 on the splendid attire section of thick bamboo 1, the output shaft of first motor 2 passes second and rotates hole 132 fastening connection and have first stirring piece 3, as shown in fig. 3, fig. 4, first stirring piece 3 includes first rotation pole 31, be equipped with first helical blade 32 and second helical blade 33 on first rotation pole 31, the spiral direction of rotation of first helical blade 32 and second helical blade 33 is the same, first stirring piece 3 is located first rotation hole 12 internal rotation through first rotation pole 31, be equipped with first belt pulley 34 on first rotation pole 31.
The second stirring part 4 which is distributed in an array mode is arranged in the containing barrel 1 in a rotating mode, as shown in fig. 3 and fig. 5, the second stirring part 4 comprises a second rotating rod 41, a third helical blade 42 is arranged on the second rotating rod 41, the blade rotating direction of the third helical blade 42 is opposite to that of the first helical blade 32, the second stirring part 4 is located in a third through hole 14 through the second rotating rod 41 to rotate, and a second belt pulley 43 is arranged on the second rotating rod 41.
The length of the second rotating lever 41 is smaller than the length of the first rotating lever 31.
The first belt pulley 34 and the second belt pulley 43 are both double-groove belts, the first belt pulley 34 is provided with a first belt 5, the first belt pulley 34 is rotatably connected with any one second belt pulley 43 through the first belt 5, the second belt pulley 43 is provided with a second belt 6, two adjacent second belt pulleys 43 are rotatably connected through the second belt 6, and the second belt pulley 43 rotatably connected with the first belt 5 is only rotatably connected with the second belt pulley 43 adjacent to one side through the second belt 6.
Be equipped with slide support member 7 on the splendid attire section of thick bamboo 1, as shown in fig. 6, be equipped with second motor 8 on the slide support member 7, slide support member 7 includes the slide rail supporting shoe 71 of symmetric distribution, be equipped with slide rail 72 on the slide rail supporting shoe 71, the both ends of slide rail supporting shoe 71 are all defeated and are equipped with dog 73, be equipped with lead screw 74 between the dog 73, be equipped with on the slide rail supporting shoe 71 and detect support member 75, it includes mutually perpendicular's second backup pad 751 and third backup pad 754 to detect support member 75, be equipped with connecting block 752 on the second backup pad 751, be equipped with screw hole 753 on the connecting block 752.
The second supporting plate 751 slides on the slide rails 72, the connecting blocks 752 are positioned between the slide rails 72 which are symmetrically distributed, the output shaft of the second motor 8 is rotatably connected with the screw rod 74, and the third supporting plate 754 is provided with an ultrasonic oscillator 9.
When the device is used, the histiocyte is conveyed into the containing cylinder main body 11 through the feeding pipe 16, quartz sand is put into the containing cylinder main body through the sand inlet hopper 15, the first motor 2 drives the first stirring piece 3 to rotate, meanwhile, the second stirring piece 4 also rotates, the radial rotating directions of the first stirring piece 3 and the second stirring piece 4 are the same, the second helical blade 33 effectively prevents the quartz sand from settling at the bottom of the containing cylinder main body 11, the rotating directions of the blades of the first helical blade 32 and the third helical blade 42 are opposite, so that the axial stirring directions of the histiocyte are opposite, the histiocyte is fully contacted with the quartz sand, and the cell crushing efficiency is improved; the ultrasonic oscillator 9 is controlled by the positive and negative rotation of the second motor 8 to move up and down, so that the efficiency of oscillating and crushing is improved.
In the description herein, references to the description of "one embodiment," "an example," "a specific example" or the like are intended to mean that a particular feature, structure, material, or characteristic described in connection with the embodiment or example is included in at least one embodiment or example of the invention. In this specification, the schematic representations of the terms used above do not necessarily refer to the same embodiment or example. Furthermore, the particular features, structures, materials, or characteristics described may be combined in any suitable manner in any one or more embodiments or examples.
The foregoing shows and describes the general principles, essential features, and advantages of the invention. It will be understood by those skilled in the art that the present invention is not limited to the embodiments described above, which are described in the specification and illustrated only to illustrate the principle of the present invention, but that various changes and modifications may be made therein without departing from the spirit and scope of the present invention, which fall within the scope of the invention as claimed.
Claims (10)
1. A crushing device for centrifugally extracting tissue cells comprises a containing cylinder (1), and is characterized in that the containing cylinder (1) comprises a containing cylinder main body (11), a first rotating hole (12) is formed in the containing cylinder main body (11), the first rotating hole (12) is communicated with the containing cylinder main body (11), a motor supporting piece (13) is arranged on the containing cylinder main body (11), a second rotating hole (132) is formed in the motor supporting piece (13), and a square through hole (131) is formed in the motor supporting piece (13);
third through holes (14) are distributed on the containing cylinder main body (11) in an array mode by taking the first rotating hole (12) as the center, a sand inlet hopper (15) is arranged on the containing cylinder main body (11), a feeding pipe (16) is arranged on the containing cylinder main body (11), a first control valve (10) is connected onto the feeding pipe (16), a discharging pipe (17) is arranged below the containing cylinder main body (11), and a second control valve (20) is arranged on the discharging pipe (17);
one side of the containing cylinder main body (11) is provided with first supporting plates (18) which are symmetrically distributed, and a mounting plate (19) is arranged between the first supporting plates (18);
the packaging barrel (1) is provided with a first motor (2), an output shaft of the first motor (2) penetrates through a second rotating hole (132) to be fixedly connected with a first stirring part (3), the first stirring part (3) comprises a first rotating rod (31), the first rotating rod (31) is provided with a first spiral blade (32) and a second spiral blade (33), and the first rotating rod (31) is provided with a first belt pulley (34);
second stirring pieces (4) distributed in an array are rotationally arranged in the containing barrel (1), each second stirring piece (4) comprises a second rotating rod (41), a third helical blade (42) is arranged on each second rotating rod (41), and a second belt pulley (43) is arranged on each second rotating rod (41);
the first belt pulley (34) and the second belt pulley (43) are both double-groove belts, a first belt (5) is arranged on the first belt pulley (34), and a second belt (6) is arranged on the second belt pulley (43);
be equipped with slide support piece (7) on splendid attire section of thick bamboo (1), be equipped with second motor (8) on slide support piece (7), slide support piece (7) are including symmetric distribution's slide rail supporting shoe (71), be equipped with slide rail (72) on slide rail supporting shoe (71), the both ends of slide rail supporting shoe (71) are all defeated and are equipped with dog (73), be equipped with lead screw (74) between dog (73), be equipped with on slide rail supporting shoe (71) and detect support piece (75), it includes mutually perpendicular's second backup pad (751) and third backup pad (754) to detect support piece (75), be equipped with connecting block (752) on second backup pad (751), be equipped with screw hole (753) on connecting block.
2. A disruption device for centrifugal extraction of tissue cells according to claim 1, wherein said second rotating aperture (132) is coaxial with said first rotating aperture (12).
3. The disruption device for centrifugal extraction of tissue cells as claimed in claim 1, wherein the direction of helical rotation of said first helical blade (32) and said second helical blade (33) is the same.
4. The disruption device for centrifugal extraction of tissue cells as claimed in claim 1, wherein said first stirring member (3) is rotated within said first rotation hole (12) by means of a first rotation rod (31).
5. The disruption device for centrifugal extraction of tissue cells as claimed in claim 1, wherein said third helical blade (42) has a blade rotation direction opposite to that of the first helical blade (32), and the second stirring member (4) is rotated in the third through-hole (14) by the second rotating shaft (41).
6. A disruption device for centrifugal extraction of tissue cells according to claim 1, wherein the length of said second rotatable shaft (41) is smaller than the length of said first rotatable shaft (31).
7. The disruption device for centrifugal extraction of tissue cells as claimed in claim 1, wherein said first pulley (34) is rotatably connected to any one of said second pulleys (43) by a first belt (5).
8. A disruption device for centrifugal extraction of tissue cells according to claim 1, wherein said two adjacent second pulleys (43) are rotatably connected by a second belt (6), wherein the second pulley (43) rotatably connected to the first belt (5) is rotatably connected to only one side adjacent second pulley (43) by the second belt (6).
9. The breaking device for centrifugal extraction of tissue cells according to claim 1, characterized in that said sliding support (7) cooperates with a screw (74) through a threaded hole (753).
10. The disruption device for centrifugal extraction of tissue cells as claimed in claim 1, wherein said second support plate (751) slides on slide rails (72), connecting blocks (752) are located between the slide rails (72) symmetrically distributed, the output shaft of the second motor (8) is rotatably connected with a screw (74), and the third support plate (754) is provided with an ultrasonic oscillator (9).
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201911247900.5A CN110903958A (en) | 2019-12-09 | 2019-12-09 | A breaker for centrifugation draws histiocyte |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201911247900.5A CN110903958A (en) | 2019-12-09 | 2019-12-09 | A breaker for centrifugation draws histiocyte |
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| Publication Number | Publication Date |
|---|---|
| CN110903958A true CN110903958A (en) | 2020-03-24 |
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| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN201911247900.5A Pending CN110903958A (en) | 2019-12-09 | 2019-12-09 | A breaker for centrifugation draws histiocyte |
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| Country | Link |
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| CN (1) | CN110903958A (en) |
Cited By (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN113637016A (en) * | 2021-08-25 | 2021-11-12 | 浙江奥托康制药集团股份有限公司 | Azathioprine tablet production process and system |
| WO2022029426A1 (en) * | 2020-08-04 | 2022-02-10 | Achilles Therapeutics Uk Limited | Disaggregation device |
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| CN209287153U (en) * | 2018-09-25 | 2019-08-23 | 浙江辉凯新材料科技有限公司 | A kind of agitating device with anti-blockage function |
| CN209322896U (en) * | 2018-08-10 | 2019-08-30 | 天津同兴讯通科技有限公司 | A kind of minicell crusher |
| CN209722169U (en) * | 2018-11-22 | 2019-12-03 | 中拓生物有限公司 | A kind of ultrasonic cell-break device enhancing percentage of damage |
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2019
- 2019-12-09 CN CN201911247900.5A patent/CN110903958A/en active Pending
Patent Citations (6)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| JP2006320888A (en) * | 2005-04-20 | 2006-11-30 | Michio Shibatani | Crushing method, crusher using the method, and crushing apparatus |
| CN208104434U (en) * | 2018-03-09 | 2018-11-16 | 广州市科沐生物科技有限公司 | A kind of broken instrument of high efficiency cell |
| CN209322896U (en) * | 2018-08-10 | 2019-08-30 | 天津同兴讯通科技有限公司 | A kind of minicell crusher |
| CN209287153U (en) * | 2018-09-25 | 2019-08-23 | 浙江辉凯新材料科技有限公司 | A kind of agitating device with anti-blockage function |
| CN209722169U (en) * | 2018-11-22 | 2019-12-03 | 中拓生物有限公司 | A kind of ultrasonic cell-break device enhancing percentage of damage |
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Cited By (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| WO2022029426A1 (en) * | 2020-08-04 | 2022-02-10 | Achilles Therapeutics Uk Limited | Disaggregation device |
| CN113637016A (en) * | 2021-08-25 | 2021-11-12 | 浙江奥托康制药集团股份有限公司 | Azathioprine tablet production process and system |
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