CN110896859A - Method for promoting fir somatic embryogenesis by using salicylic acid - Google Patents
Method for promoting fir somatic embryogenesis by using salicylic acid Download PDFInfo
- Publication number
- CN110896859A CN110896859A CN201911196614.0A CN201911196614A CN110896859A CN 110896859 A CN110896859 A CN 110896859A CN 201911196614 A CN201911196614 A CN 201911196614A CN 110896859 A CN110896859 A CN 110896859A
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- CN
- China
- Prior art keywords
- fir
- salicylic acid
- culture medium
- somatic
- promoting
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- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
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- YGSDEFSMJLZEOE-UHFFFAOYSA-N salicylic acid Chemical compound OC(=O)C1=CC=CC=C1O YGSDEFSMJLZEOE-UHFFFAOYSA-N 0.000 title claims abstract description 86
- FJKROLUGYXJWQN-UHFFFAOYSA-N papa-hydroxy-benzoic acid Natural products OC(=O)C1=CC=C(O)C=C1 FJKROLUGYXJWQN-UHFFFAOYSA-N 0.000 title claims abstract description 43
- 229960004889 salicylic acid Drugs 0.000 title claims abstract description 43
- 238000000034 method Methods 0.000 title claims abstract description 24
- 230000030118 somatic embryogenesis Effects 0.000 title claims abstract description 23
- 230000001737 promoting effect Effects 0.000 title claims abstract description 13
- 210000001161 mammalian embryo Anatomy 0.000 claims abstract description 41
- 210000002257 embryonic structure Anatomy 0.000 claims abstract description 35
- 230000000392 somatic effect Effects 0.000 claims abstract description 33
- 230000006698 induction Effects 0.000 claims abstract description 31
- 239000001963 growth medium Substances 0.000 claims abstract description 25
- 206010020649 Hyperkeratosis Diseases 0.000 claims abstract description 11
- 238000012258 culturing Methods 0.000 claims abstract description 4
- 239000005018 casein Substances 0.000 claims description 6
- BECPQYXYKAMYBN-UHFFFAOYSA-N casein, tech. Chemical compound NCCCCC(C(O)=O)N=C(O)C(CC(O)=O)N=C(O)C(CCC(O)=N)N=C(O)C(CC(C)C)N=C(O)C(CCC(O)=O)N=C(O)C(CC(O)=O)N=C(O)C(CCC(O)=O)N=C(O)C(C(C)O)N=C(O)C(CCC(O)=N)N=C(O)C(CCC(O)=N)N=C(O)C(CCC(O)=N)N=C(O)C(CCC(O)=O)N=C(O)C(CCC(O)=O)N=C(O)C(COP(O)(O)=O)N=C(O)C(CCC(O)=N)N=C(O)C(N)CC1=CC=CC=C1 BECPQYXYKAMYBN-UHFFFAOYSA-N 0.000 claims description 6
- 235000021240 caseins Nutrition 0.000 claims description 6
- ZDXPYRJPNDTMRX-UHFFFAOYSA-N glutamine Natural products OC(=O)C(N)CCC(N)=O ZDXPYRJPNDTMRX-UHFFFAOYSA-N 0.000 claims description 6
- 229920001817 Agar Polymers 0.000 claims description 5
- 239000008272 agar Substances 0.000 claims description 5
- 238000005520 cutting process Methods 0.000 claims description 5
- 239000002609 medium Substances 0.000 claims description 5
- PZSMUPGANZGPBF-UHFFFAOYSA-N 4-[5-(dithiolan-3-yl)pentanoylamino]butanoic acid Chemical compound OC(=O)CCCNC(=O)CCCCC1CCSS1 PZSMUPGANZGPBF-UHFFFAOYSA-N 0.000 claims description 3
- DCXYFEDJOCDNAF-UHFFFAOYSA-N Asparagine Natural products OC(=O)C(N)CC(N)=O DCXYFEDJOCDNAF-UHFFFAOYSA-N 0.000 claims description 3
- DCXYFEDJOCDNAF-REOHCLBHSA-N L-asparagine Chemical compound OC(=O)[C@@H](N)CC(N)=O DCXYFEDJOCDNAF-REOHCLBHSA-N 0.000 claims description 3
- ONIBWKKTOPOVIA-UHFFFAOYSA-N Proline Natural products OC(=O)C1CCCN1 ONIBWKKTOPOVIA-UHFFFAOYSA-N 0.000 claims description 3
- 229960001230 asparagine Drugs 0.000 claims description 3
- 235000009582 asparagine Nutrition 0.000 claims description 3
- 229940125389 long-acting beta agonist Drugs 0.000 claims description 3
- 230000001954 sterilising effect Effects 0.000 claims description 3
- 244000050510 Cunninghamia lanceolata Species 0.000 abstract description 6
- 230000001976 improved effect Effects 0.000 abstract description 4
- 238000004161 plant tissue culture Methods 0.000 abstract description 2
- 230000000408 embryogenic effect Effects 0.000 description 7
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- 241000196324 Embryophyta Species 0.000 description 3
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- 241000218645 Cedrus Species 0.000 description 2
- 241000218631 Coniferophyta Species 0.000 description 2
- 238000010306 acid treatment Methods 0.000 description 2
- 230000015572 biosynthetic process Effects 0.000 description 2
- 210000004027 cell Anatomy 0.000 description 2
- 238000010586 diagram Methods 0.000 description 2
- 230000000694 effects Effects 0.000 description 2
- 230000013020 embryo development Effects 0.000 description 2
- 238000004519 manufacturing process Methods 0.000 description 2
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- 239000012452 mother liquor Substances 0.000 description 2
- 238000009331 sowing Methods 0.000 description 2
- 239000007858 starting material Substances 0.000 description 2
- 230000004554 suspensor development Effects 0.000 description 2
- 241000218642 Abies Species 0.000 description 1
- 235000014466 Douglas bleu Nutrition 0.000 description 1
- 244000146486 Glehnia littoralis Species 0.000 description 1
- 235000004036 Glehnia littoralis Nutrition 0.000 description 1
- 241000218922 Magnoliophyta Species 0.000 description 1
- 235000011334 Pinus elliottii Nutrition 0.000 description 1
- 241000142776 Pinus elliottii Species 0.000 description 1
- 241000218683 Pseudotsuga Species 0.000 description 1
- 240000001416 Pseudotsuga menziesii Species 0.000 description 1
- 235000008572 Pseudotsuga menziesii Nutrition 0.000 description 1
- 235000005386 Pseudotsuga menziesii var menziesii Nutrition 0.000 description 1
- 241001149649 Taxus wallichiana var. chinensis Species 0.000 description 1
- 229920002522 Wood fibre Polymers 0.000 description 1
- 238000004458 analytical method Methods 0.000 description 1
- 238000013459 approach Methods 0.000 description 1
- 230000004071 biological effect Effects 0.000 description 1
- 230000035584 blastogenesis Effects 0.000 description 1
- 230000000052 comparative effect Effects 0.000 description 1
- 238000005260 corrosion Methods 0.000 description 1
- 230000007797 corrosion Effects 0.000 description 1
- 238000005336 cracking Methods 0.000 description 1
- 210000002308 embryonic cell Anatomy 0.000 description 1
- 238000005516 engineering process Methods 0.000 description 1
- 230000002349 favourable effect Effects 0.000 description 1
- 230000004720 fertilization Effects 0.000 description 1
- 239000003205 fragrance Substances 0.000 description 1
- 230000002068 genetic effect Effects 0.000 description 1
- 230000035784 germination Effects 0.000 description 1
- 230000001939 inductive effect Effects 0.000 description 1
- 238000012423 maintenance Methods 0.000 description 1
- 230000035800 maturation Effects 0.000 description 1
- 239000010413 mother solution Substances 0.000 description 1
- 238000001543 one-way ANOVA Methods 0.000 description 1
- 230000037361 pathway Effects 0.000 description 1
- 239000000843 powder Substances 0.000 description 1
- 238000012545 processing Methods 0.000 description 1
- 230000000644 propagated effect Effects 0.000 description 1
- 239000002994 raw material Substances 0.000 description 1
- 230000008929 regeneration Effects 0.000 description 1
- 238000011069 regeneration method Methods 0.000 description 1
- 238000011160 research Methods 0.000 description 1
- 241000894007 species Species 0.000 description 1
- 238000012360 testing method Methods 0.000 description 1
- 229910021642 ultra pure water Inorganic materials 0.000 description 1
- 239000012498 ultrapure water Substances 0.000 description 1
- XLYOFNOQVPJJNP-UHFFFAOYSA-N water Substances O XLYOFNOQVPJJNP-UHFFFAOYSA-N 0.000 description 1
- 239000002023 wood Substances 0.000 description 1
- 239000002025 wood fiber Substances 0.000 description 1
Images
Classifications
-
- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01H—NEW PLANTS OR NON-TRANSGENIC PROCESSES FOR OBTAINING THEM; PLANT REPRODUCTION BY TISSUE CULTURE TECHNIQUES
- A01H4/00—Plant reproduction by tissue culture techniques ; Tissue culture techniques therefor
- A01H4/001—Culture apparatus for tissue culture
-
- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01H—NEW PLANTS OR NON-TRANSGENIC PROCESSES FOR OBTAINING THEM; PLANT REPRODUCTION BY TISSUE CULTURE TECHNIQUES
- A01H4/00—Plant reproduction by tissue culture techniques ; Tissue culture techniques therefor
- A01H4/005—Methods for micropropagation; Vegetative plant propagation using cell or tissue culture techniques
- A01H4/006—Encapsulated embryos for plant reproduction, e.g. artificial seeds
Landscapes
- Life Sciences & Earth Sciences (AREA)
- Developmental Biology & Embryology (AREA)
- Engineering & Computer Science (AREA)
- Biotechnology (AREA)
- Cell Biology (AREA)
- Botany (AREA)
- Environmental Sciences (AREA)
- Breeding Of Plants And Reproduction By Means Of Culturing (AREA)
Abstract
Description
Claims (6)
Priority Applications (2)
Application Number | Priority Date | Filing Date | Title |
---|---|---|---|
CN201911196614.0A CN110896859B (en) | 2019-11-28 | 2019-11-28 | Method for promoting fir somatic embryogenesis by using salicylic acid |
PCT/CN2019/125020 WO2021103168A1 (en) | 2019-11-28 | 2019-12-13 | Method for promoting fir somatic embryogenesis by using salicylic acid |
Applications Claiming Priority (1)
Application Number | Priority Date | Filing Date | Title |
---|---|---|---|
CN201911196614.0A CN110896859B (en) | 2019-11-28 | 2019-11-28 | Method for promoting fir somatic embryogenesis by using salicylic acid |
Publications (2)
Publication Number | Publication Date |
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CN110896859A true CN110896859A (en) | 2020-03-24 |
CN110896859B CN110896859B (en) | 2021-06-18 |
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Family Applications (1)
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CN201911196614.0A Active CN110896859B (en) | 2019-11-28 | 2019-11-28 | Method for promoting fir somatic embryogenesis by using salicylic acid |
Country Status (2)
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CN (1) | CN110896859B (en) |
WO (1) | WO2021103168A1 (en) |
Cited By (1)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
CN115735769A (en) * | 2022-11-25 | 2023-03-07 | 南京林业大学 | Method for promoting fir somatic embryogenesis by controlling redox homeostasis |
Families Citing this family (1)
Publication number | Priority date | Publication date | Assignee | Title |
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EP4197318A1 (en) | 2021-12-17 | 2023-06-21 | Albert-Ludwigs-Universität Freiburg | Phosphodiesterases inhibitors to promote in vitro plant cell reprogramming towards plant embryogenesis or microcallus formation |
Citations (1)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
CN101983556A (en) * | 2010-09-07 | 2011-03-09 | 南京林业大学 | Spruce somatic embryogenesis and plant regeneration method |
Family Cites Families (3)
Publication number | Priority date | Publication date | Assignee | Title |
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CN101130763B (en) * | 2007-08-08 | 2011-05-25 | 南京林业大学 | Rapid breeding method for gingkgo callus or suspending cell |
CN106538394A (en) * | 2016-12-20 | 2017-03-29 | 江西宜信堂医疗科技有限公司 | A kind of Ramulus et folium taxi cuspidatae culture medium and preparation method thereof |
CN106688895B (en) * | 2017-03-27 | 2018-11-27 | 南京林业大学 | A method of the generation of hybridized Chinese tuliptree body embryo is carried out using salicylic acid |
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2019
- 2019-11-28 CN CN201911196614.0A patent/CN110896859B/en active Active
- 2019-12-13 WO PCT/CN2019/125020 patent/WO2021103168A1/en active Application Filing
Patent Citations (1)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
CN101983556A (en) * | 2010-09-07 | 2011-03-09 | 南京林业大学 | Spruce somatic embryogenesis and plant regeneration method |
Cited By (2)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
CN115735769A (en) * | 2022-11-25 | 2023-03-07 | 南京林业大学 | Method for promoting fir somatic embryogenesis by controlling redox homeostasis |
CN115735769B (en) * | 2022-11-25 | 2023-09-08 | 南京林业大学 | Method for promoting embryogenesis of fir wood by controlling redox homeostasis |
Also Published As
Publication number | Publication date |
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WO2021103168A1 (en) | 2021-06-03 |
CN110896859B (en) | 2021-06-18 |
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Application publication date: 20200324 Assignee: Yixun biotechnology Nanjing Co.,Ltd. Assignor: NANJING FORESTRY University Contract record no.: X2021980013806 Denomination of invention: A method of promoting somatic embryogenesis of Chinese fir by salicylic acid Granted publication date: 20210618 License type: Common License Record date: 20211201 |
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Application publication date: 20200324 Assignee: Nanjing Baikang Biotechnology Co.,Ltd. Assignor: NANJING FORESTRY University Contract record no.: X2021980013901 Denomination of invention: A method of promoting somatic embryogenesis of Chinese fir by salicylic acid Granted publication date: 20210618 License type: Common License Record date: 20211202 |
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Application publication date: 20200324 Assignee: Fujian Jinshuo Biotechnology Co.,Ltd. Assignor: NANJING FORESTRY University Contract record no.: X2021320000148 Denomination of invention: A method of promoting somatic embryogenesis of Chinese fir by salicylic acid Granted publication date: 20210618 License type: Common License Record date: 20211215 |
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Assignee: Fujian Jinshuo Biotechnology Co.,Ltd. Assignor: NANJING FORESTRY University Contract record no.: X2021320000148 Date of cancellation: 20230210 |
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