CN100433971C - Method for regenerating bitter herbs corm by in-vitro culture - Google Patents
Method for regenerating bitter herbs corm by in-vitro culture Download PDFInfo
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- CN100433971C CN100433971C CNB2006100393449A CN200610039344A CN100433971C CN 100433971 C CN100433971 C CN 100433971C CN B2006100393449 A CNB2006100393449 A CN B2006100393449A CN 200610039344 A CN200610039344 A CN 200610039344A CN 100433971 C CN100433971 C CN 100433971C
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- bitter herbs
- corm
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- bitter
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- 235000008216 herbs Nutrition 0.000 title claims abstract description 14
- 238000000034 method Methods 0.000 title claims abstract description 9
- 238000000338 in vitro Methods 0.000 title claims abstract description 6
- 230000001172 regenerating effect Effects 0.000 title abstract description 3
- 241000196324 Embryophyta Species 0.000 claims description 20
- 241001123263 Zostera Species 0.000 claims description 19
- 206010020649 Hyperkeratosis Diseases 0.000 claims description 16
- 239000005556 hormone Substances 0.000 claims description 14
- 229940088597 hormone Drugs 0.000 claims description 14
- 230000008929 regeneration Effects 0.000 claims description 12
- 238000011069 regeneration method Methods 0.000 claims description 12
- 230000006698 induction Effects 0.000 claims description 8
- 229920002148 Gellan gum Polymers 0.000 claims description 4
- 230000001939 inductive effect Effects 0.000 claims description 4
- 230000000763 evoking effect Effects 0.000 claims description 3
- CZMRCDWAGMRECN-UGDNZRGBSA-N Sucrose Chemical compound O[C@H]1[C@H](O)[C@@H](CO)O[C@@]1(CO)O[C@@H]1[C@H](O)[C@@H](O)[C@H](O)[C@@H](CO)O1 CZMRCDWAGMRECN-UGDNZRGBSA-N 0.000 claims description 2
- 229930006000 Sucrose Natural products 0.000 claims description 2
- 239000005720 sucrose Substances 0.000 claims description 2
- 239000011573 trace mineral Substances 0.000 claims description 2
- 235000013619 trace mineral Nutrition 0.000 claims description 2
- 239000011782 vitamin Substances 0.000 claims description 2
- 229940088594 vitamin Drugs 0.000 claims description 2
- 229930003231 vitamin Natural products 0.000 claims description 2
- 235000013343 vitamin Nutrition 0.000 claims description 2
- 150000003722 vitamin derivatives Chemical class 0.000 claims description 2
- XLYOFNOQVPJJNP-UHFFFAOYSA-N water Substances O XLYOFNOQVPJJNP-UHFFFAOYSA-N 0.000 abstract description 10
- 239000002689 soil Substances 0.000 abstract description 6
- 238000012851 eutrophication Methods 0.000 abstract description 4
- 230000004083 survival effect Effects 0.000 abstract description 3
- 238000004161 plant tissue culture Methods 0.000 abstract description 2
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- 230000035784 germination Effects 0.000 abstract 1
- 235000013311 vegetables Nutrition 0.000 abstract 1
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- 230000018109 developmental process Effects 0.000 description 3
- 238000011160 research Methods 0.000 description 3
- 238000003911 water pollution Methods 0.000 description 3
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- 238000011031 large-scale manufacturing process Methods 0.000 description 2
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- 241000272778 Cygnus atratus Species 0.000 description 1
- 241001113438 Vallisneria Species 0.000 description 1
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- Breeding Of Plants And Reproduction By Means Of Culturing (AREA)
Abstract
The present invention belongs to the technical field of plant tissue culture. The excellent cultivating seedlings of bitter herbs are produced in a large-scale way by the method for regenerating bitter herbs corm by in-vitro culture. The present invention can overcome the defects that vegetable seed seedlings have low survival rate under the natural condition, the germination of winter buds is largely influenced by the thickness of the soil layer, and a large number of live winter buds in the soil layer can not be germinated to strains. The present invention can satisfy the active demands for reconstructing domestic aquatic vegetation, reducing water body pollution, and releasing water eutrophication.
Description
One, technical field
The invention belongs to field of plant tissue culture technique.
Two, background technology
China's aquatic ecosystem seriously polluted, the decline of quality of water environment has caused heavy damage to living aquatic resources, recover the balance of aquatic ecosystem, purifies water, and promotes fisheries development, introduces a fine variety the water body purification plant, and is significant.
Eel grass is heavy this plant of pasture and water of Hydrocharctaceae Hydrocharitaceae Vallisneria Vallisneria, and eel grass is nutritious, composition is complete, is the important bait of herbivorous fishes and zoobenthos; The eel grass hibernaculum is the important foodstuffs that some wild rare birds survive the winter period; The eel grass annidation is wide, and absorption dirt ability is strong, is to reduce water pollution, alleviates the important submerged plant of water body eutrophication degree; The destroyed back of its plant corpus is short recovery time, thereby is again the emphasis alternative of rebuilding aquatic vegetation.But it is low that this plant seed seedling is settled down survival rate under field conditions (factors), and hibernaculum sprouts and is subjected to the influence of soil thickness big, and life hibernaculums a large amount of in the soil layer can not sprout into the eel grass plant, is difficult to large-scale breeding and promotes.
Still the relevant report of not having the research of eel grass tissue culture technique at present both at home and abroad.
Three, summary of the invention
The objective of the invention is: the method for inventing a kind of large-scale production eel grass high-quality tissue cultivating seedling, to settle down survival rate under field conditions (factors) low to overcome this plant seed seedling, hibernaculum sprouts and is subjected to the influence of soil thickness big, life hibernaculums a large amount of in the soil layer can not sprout into the eel grass plant, the drawback that the retardance large-scale breeding is promoted, adapting to China rebuilds in the aquatic vegetation work, emphasis selects eel grass to reduce water pollution, what the alleviation water body eutrophication degree was introduced a fine variety presses for, thereby recover good aquatic ecological environment, promote China's sustainable development of fishery.
Technical scheme of the present invention is:
Selection, adjustment by different explants improve the factors such as explant sterilization treatment conditions and medium state, are obtaining success aspect brownization of checking explant and the pollution.Selecting suitable explant and setting up sterile system is the key that can the work of decision tissue culture be carried out.
Utilize bitter herbs corm to carry out callus induction and cultivate,, its callus induction frequency is reached more than 80% by the selection and the hormone regulating and controlling of medium.
From the callus regeneration whole plant is the key of tissue-culturing rapid propagation success.By adjusting the factors such as hormone condition, nitrogenous source and organic compound, obtained the eel grass regeneration plant.
The present invention has set up eel grass callus regeneration system, changes differentiation over to from callus, and plant regeneration frequency is 90%.
On the basis of above research, the propagation method that this invention has been set up bitter herbs corm cultured in vitro regrowth is as follows:
(1) adding 2, under the 4-D hormone condition by the bitter herbs corm evoked callus;
(2) adding under the BA hormone condition inducing culture regeneration plant;
(3) adding under the NAA hormone condition, turn out the complete regeneration plant of taking root;
This propagation method stable performance, the test good reproducibility, the test tube regrowth breaks up, the proterties of growing thickly is good.The medium of each cultivation stage and hormone combination such as following table:
The regenerate hormone in medium prescription in each stage of bitter herbs corm cultured in vitro
| The group training stage | The hormone in medium composition |
| Callus induction | 2，4-D 0.5mg/L |
| Plant regeneration | BA2.0mg/L， |
| Plant takes root | NAA 0.1mg/L，IBA0.1mg/L， |
In the test-tube plantlet production process, the minimal medium of each cultivation stage is MS macroelement, trace element, B5 vitamin, sucrose 3%, Gelrite or Phytagel 0.2%.
Adopting this technical system, is explant with the bitter herbs corm, and callus induction rate is more than 80%; The frequency of callus plantlet reaches 90%; The rooting rate of test-tube plantlet reaches 100%.
Effect of the present invention is: adopting this technical system, is explant with the bulb, and callus induction rate is more than 80%; The frequency of callus plantlet reaches 90%; The rooting rate of test-tube plantlet reaches 100%.
Inducing culture research by somatic embryo, the test-tube plantlet of large-scale production eel grass, and, be beneficial to promotion development with the agronomy quality of technological means such as tissue culture improvement eel grass.Eel grass is nutritious, composition is complete, is the important bait of herbivorous fishes and zoobenthos; Its hibernaculum is Fresh ﹠ Tender in Texture, is the good food item of wild bird, particularly some rare birds such as white stork, the important foodstuffs in the periods of surviving the winter such as black stork, swan and hoary hair crane, and in addition, according to the Compendium of Material Medica record, eel grass has obvious medicinal value; The eel grass part of growing thickly is the good habitat and the first-class farmland green manure of crab class; The more important thing is that the eel grass annidation is wide, absorption dirt ability is strong, is to reduce water pollution, alleviates the important submerged plant of water body eutrophication degree; The destroyed back of its plant corpus is short recovery time, thereby is again the emphasis alternative of rebuilding aquatic vegetation.Have good feed concurrently, recover the characteristics of aquatic vegetation and purifying water body, the demand side that make it in the lake, continuable fishery system is developed in the pond will be accomplished something.
Four, embodiment
This method is carried out in the Plant Tissue Breeding chamber, has successively cultivated three batches of about 12000 strain eel grass engineering seedlings.Its concrete mode is: based on the MS minimal medium, utilize bitter herbs corm to carry out callus induction and cultivate, adding 2, by the bitter herbs corm evoked callus, its callus induction frequency reaches more than 80% under the 4-D hormone condition.Adding under the BA hormone condition inducing culture regeneration plant; Plant regeneration frequency is 90%.Adding under NAA and the IBA hormone condition, turn out the complete regeneration plant of taking root; The rooting rate of test-tube plantlet reaches 100%.
Claims (1)
1, a kind of bitter herbs corm cultured in vitro propagation method, it is characterized in that utilizing bitter herbs corm to carry out callus induction cultivates, turn out complete eel grass test-tube plantlet, its concrete grammar is: with MS macroelement, trace element, B5 vitamin, sucrose 3%, Gelrite or Phytagel 0.2% medium are the basis, adding 0.5mg/L 2, under the 4-D hormone condition by the bitter herbs corm evoked callus; Adding under the 2.0mg/L BA hormone condition inducing culture regeneration plant; Adding under 0.1mg/L IBA and the 0.1mg/L NAA hormone condition, turn out the complete regeneration plant of taking root.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CNB2006100393449A CN100433971C (en) | 2006-04-06 | 2006-04-06 | Method for regenerating bitter herbs corm by in-vitro culture |
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| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CNB2006100393449A CN100433971C (en) | 2006-04-06 | 2006-04-06 | Method for regenerating bitter herbs corm by in-vitro culture |
Publications (2)
| Publication Number | Publication Date |
|---|---|
| CN1826877A CN1826877A (en) | 2006-09-06 |
| CN100433971C true CN100433971C (en) | 2008-11-19 |
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| CNB2006100393449A Expired - Fee Related CN100433971C (en) | 2006-04-06 | 2006-04-06 | Method for regenerating bitter herbs corm by in-vitro culture |
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Families Citing this family (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN102577936B (en) * | 2012-02-14 | 2014-04-23 | 上海市崇明水生水环境研究所 | Seed selection method for short flower-free tape grass |
| CN107333658A (en) * | 2017-09-13 | 2017-11-10 | 中冶华天工程技术有限公司 | A kind of quick-breeding method of the close thorn eel grass engineering seedling of hormone induction |
Citations (4)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN1426966A (en) * | 2001-12-17 | 2003-07-02 | 中国科学院武汉植物研究所 | Method of restoring nutrition enriched shallow lake aquatic vegetation |
| CN1586174A (en) * | 2004-07-23 | 2005-03-02 | 中国科学院武汉植物园 | Method for quick breeding eel grass |
| CN1590319A (en) * | 2003-08-28 | 2005-03-09 | 陈卫民 | Method of making lake rich nutrition using eel grass |
| CN1623374A (en) * | 2004-12-14 | 2005-06-08 | 南京大学 | Eel grass root stem sterile culturing and engineering seedling fast reproducing method |
-
2006
- 2006-04-06 CN CNB2006100393449A patent/CN100433971C/en not_active Expired - Fee Related
Patent Citations (4)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN1426966A (en) * | 2001-12-17 | 2003-07-02 | 中国科学院武汉植物研究所 | Method of restoring nutrition enriched shallow lake aquatic vegetation |
| CN1590319A (en) * | 2003-08-28 | 2005-03-09 | 陈卫民 | Method of making lake rich nutrition using eel grass |
| CN1586174A (en) * | 2004-07-23 | 2005-03-02 | 中国科学院武汉植物园 | Method for quick breeding eel grass |
| CN1623374A (en) * | 2004-12-14 | 2005-06-08 | 南京大学 | Eel grass root stem sterile culturing and engineering seedling fast reproducing method |
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| CN1826877A (en) | 2006-09-06 |
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Granted publication date: 20081119 Termination date: 20170406 |