CN110887291B - A cold storage plant for stem cell culture - Google Patents

A cold storage plant for stem cell culture Download PDF

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Publication number
CN110887291B
CN110887291B CN201911205006.1A CN201911205006A CN110887291B CN 110887291 B CN110887291 B CN 110887291B CN 201911205006 A CN201911205006 A CN 201911205006A CN 110887291 B CN110887291 B CN 110887291B
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China
Prior art keywords
refrigerating
refrigerator
pipe
groove
stem cell
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CN201911205006.1A
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Chinese (zh)
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CN110887291A (en
Inventor
王冬
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Anhui From Biological Technology Co ltd
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Anhui From Biological Technology Co ltd
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    • FMECHANICAL ENGINEERING; LIGHTING; HEATING; WEAPONS; BLASTING
    • F25REFRIGERATION OR COOLING; COMBINED HEATING AND REFRIGERATION SYSTEMS; HEAT PUMP SYSTEMS; MANUFACTURE OR STORAGE OF ICE; LIQUEFACTION SOLIDIFICATION OF GASES
    • F25DREFRIGERATORS; COLD ROOMS; ICE-BOXES; COOLING OR FREEZING APPARATUS NOT OTHERWISE PROVIDED FOR
    • F25D11/00Self-contained movable devices, e.g. domestic refrigerators
    • AHUMAN NECESSITIES
    • A01AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
    • A01NPRESERVATION OF BODIES OF HUMANS OR ANIMALS OR PLANTS OR PARTS THEREOF; BIOCIDES, e.g. AS DISINFECTANTS, AS PESTICIDES OR AS HERBICIDES; PEST REPELLANTS OR ATTRACTANTS; PLANT GROWTH REGULATORS
    • A01N1/00Preservation of bodies of humans or animals, or parts thereof
    • A01N1/02Preservation of living parts
    • A01N1/0236Mechanical aspects
    • A01N1/0242Apparatuses, i.e. devices used in the process of preservation of living parts, such as pumps, refrigeration devices or any other devices featuring moving parts and/or temperature controlling components
    • A01N1/0252Temperature controlling refrigerating apparatus, i.e. devices used to actively control the temperature of a designated internal volume, e.g. refrigerators, freeze-drying apparatus or liquid nitrogen baths
    • A01N1/0257Stationary or portable vessels generating cryogenic temperatures
    • FMECHANICAL ENGINEERING; LIGHTING; HEATING; WEAPONS; BLASTING
    • F25REFRIGERATION OR COOLING; COMBINED HEATING AND REFRIGERATION SYSTEMS; HEAT PUMP SYSTEMS; MANUFACTURE OR STORAGE OF ICE; LIQUEFACTION SOLIDIFICATION OF GASES
    • F25DREFRIGERATORS; COLD ROOMS; ICE-BOXES; COOLING OR FREEZING APPARATUS NOT OTHERWISE PROVIDED FOR
    • F25D23/00General constructional features
    • F25D23/02Doors; Covers
    • FMECHANICAL ENGINEERING; LIGHTING; HEATING; WEAPONS; BLASTING
    • F25REFRIGERATION OR COOLING; COMBINED HEATING AND REFRIGERATION SYSTEMS; HEAT PUMP SYSTEMS; MANUFACTURE OR STORAGE OF ICE; LIQUEFACTION SOLIDIFICATION OF GASES
    • F25DREFRIGERATORS; COLD ROOMS; ICE-BOXES; COOLING OR FREEZING APPARATUS NOT OTHERWISE PROVIDED FOR
    • F25D29/00Arrangement or mounting of control or safety devices

Abstract

The invention discloses a refrigerating device for stem cell culture, which comprises a cabinet body, wherein refrigerating grooves distributed in an array manner are arranged on one side of the cabinet body, a temperature control switch is arranged on any refrigerating groove, placing grooves distributed in an array manner are arranged on the opposite side of the refrigerating groove arranged on the cabinet body, the placing grooves and the refrigerating grooves are distributed in a cross manner, and an equipment groove is arranged below the placing grooves. The refrigerating device absorbs heat through the hydrogen discharge reaction of the lanthanum-nickel-tin alloy powder to refrigerate the refrigerating groove, the temperature in the refrigerating groove is detected through the temperature control switch, the hydrogen pump sucks hydrogen into the hydrogen pump, and the negative pressure in the refrigerator is controlled by adjusting the rotating speed of the hydrogen pump, so that the temperature is controlled; replace traditional freon refrigeration, the refrigerated speed of the dehydrogenation reaction of lanthanum nickel tin alloy powder is very fast, and sensitivity is high, is favorable to placing test tube or stem cell transfer bag, and refrigerated effect and sensitivity are high.

Description

A cold storage plant for stem cell culture
Technical Field
The invention relates to a refrigerating device, in particular to a refrigerating device for stem cell culture.
Background
The biotechnology is that people use modern life science as a basis, combine with the scientific principle of other basic sciences, adopt advanced scientific and technological means, reform organism or process biological raw materials according to the design in advance, can produce the desired product or achieve a certain purpose for the human beings, modern biotechnology is comprehensive to have multidisciplinary technologies such as genetic engineering, molecular biology, biochemistry, genetics, cell biology, embryology, etc.; the cell biology is a science for researching the structure, the function and various life laws of cells on the three levels of microscopy, submicroscopic level and molecular level, and the modern cell biology researches the structure, the function and the life activities of the cells from different levels of microscopy level, ultramicro level, molecular level and the like; in the development and planning of basic disciplines in China, cell biology, molecular biology, neurobiology and ecology are combined into four basic disciplines of life science.
Clinical research and treatment of cells, need to have independent cell preparation unit to provide the cell, will provide the cell for cell use unit, make the cell whether research and development or clinical application reach the best vitality, need to have the standardized cell that can reach the standard to store the transportation flow, the primitive stem cell that needs to store the transportation is through long-time culture in vitro, through digestion many times, passage, washing processing, based on the trade particularity of the storage transportation of stem cell, need to carry out cold storage processing to stem cell, stem cell is inconvenient to carry among the prior art, it is also inconvenient to take, and current fridge mainly passes through freon, refrigerated speed is slower, the sensitivity is lower, be unfavorable for placing test tube or stem cell transfer bag, refrigerated effect and sensitivity are not high.
Disclosure of Invention
The invention aims to provide a refrigerating device for stem cell culture, which is characterized in that stem cells are placed in a refrigerating tank, a second electromagnetic valve is closed, a first electromagnetic valve is opened, a hydrogen pump works to generate negative pressure in a refrigerator, so that dehydrogenation reaction of lanthanum-nickel-tin alloy powder occurs, heat is absorbed through the hydrogen discharge reaction of the lanthanum-nickel-tin alloy powder, the refrigerating tank is refrigerated, the temperature in the refrigerating tank is detected through a temperature control switch, the hydrogen pump sucks hydrogen into the hydrogen pump, and the negative pressure in the refrigerator is controlled by adjusting the rotating speed of the hydrogen pump, so that the temperature control is realized, the traditional Freon refrigeration is replaced, the speed of the dehydrogenation reaction refrigeration of the lanthanum-nickel-tin alloy powder is higher, the sensitivity is high, a test tube or a stem cell transfer bag can be placed conveniently, and the refrigerating effect and the sensitivity are high.
The purpose of the invention can be realized by the following technical scheme:
the utility model provides a cold storage plant for stem cell culture, cold storage plant includes the cabinet body, one side of the cabinet body is equipped with the cold-stored groove of array distribution, is equipped with temperature detect switch on arbitrary one cold-stored groove.
The opposite side that is equipped with the refrigerated groove on the cabinet body is equipped with the standing groove of array distribution, standing groove and refrigerated groove cross distribution, the below of standing groove is equipped with the equipment groove.
One side of the cabinet body is provided with an electric control unit, one side of the cabinet body, which is provided with a placing groove, is provided with a first door body, and the other side is provided with a second door body.
And a placing rack for placing a container for containing the stem cells is arranged in the refrigerating groove.
The cabinet is internally provided with a refrigerating piece, and the refrigerating piece comprises refrigerators, a first communicating pipe, a second communicating pipe, a hydrogen tank, a hydrogen pump, a first electromagnetic valve and a second electromagnetic valve which are distributed in an array manner.
The refrigerators are distributed in an S shape and comprise containing pipe bodies distributed in an array mode.
Both ends of splendid attire body all are equipped with the communicating piece, are equipped with sealed piece in the communicating piece, are equipped with the trigger tube that the array distributes between two sealed pieces, are equipped with the intercommunicating pore that the array distributes on the trigger tube, and the intercommunicating pore intercommunication splendid attire body and trigger tube are equipped with lanthanum nickel tin alloy powder in the splendid attire body.
Further, the notch positions of the two adjacent refrigerating grooves are communicated with each other.
Further, the placing groove is positioned below the refrigerating groove.
Furthermore, one end of the refrigerator is communicated with the first communicating pipe, and the other end of the refrigerator is communicated with the second communicating pipe.
Furthermore, one end of the first communicating pipe is closed, the other end of the first communicating pipe is communicated with the hydrogen tank, and the second electromagnetic valve is located on the pipe body between the first communicating pipe and the hydrogen tank and controls the connection and disconnection of the first communicating pipe and the hydrogen tank.
Furthermore, one end of the hydrogen pump is communicated with the hydrogen tank, the other end of the hydrogen pump is communicated with one end of the second communicating pipe, and the other end of the second communicating pipe is closed.
Further, the first electromagnetic valve is located on a pipe body communicated with the hydrogen pump and the second communicating pipe.
Further, adjacent splendid attire body head and the tail communicate in proper order, and the splendid attire body other end of head and the tail all is equipped with third communicating pipe, and the refrigerator communicates with first communicating pipe through the third communicating pipe of one side, communicates with second communicating pipe through the third communicating pipe of opposite side.
Furthermore, lanthanum nickel tin alloy powder is contained in the containing pipe body, and the fineness of the lanthanum nickel tin alloy powder is larger than that of the intercommunicating pores.
Furthermore, the first electromagnetic valve, the second electromagnetic valve, the hydrogen pump and the temperature control switch are electrically connected with the electric control unit.
The invention has the beneficial effects that:
1. the refrigeration device comprises a refrigeration device, a first electromagnetic valve, a hydrogen pump, a temperature control switch, a hydrogen pump and a second electromagnetic valve, wherein the refrigeration device is used for placing stem cells in a refrigeration tank, closing the second electromagnetic valve, opening the first electromagnetic valve, enabling the hydrogen pump to work, generating negative pressure in a refrigerator, enabling lanthanum-nickel-tin alloy powder to generate dehydrogenation reaction, absorbing heat through the hydrogen discharge reaction of the lanthanum-nickel-tin alloy powder, refrigerating the refrigeration tank, detecting the temperature in the refrigeration tank through the temperature control switch, enabling the hydrogen pump to suck hydrogen into the hydrogen pump, and controlling the negative pressure in the refrigerator by adjusting the rotating speed of the hydrogen pump, so;
2. the refrigerating device of the invention replaces the traditional Freon refrigeration, the dehydrogenation reaction refrigeration of the lanthanum nickel tin alloy powder has higher refrigeration speed and high sensitivity, is beneficial to placing test tubes or stem cell transfer bags, and has high refrigeration effect and sensitivity.
Drawings
The invention will be further described with reference to the accompanying drawings.
FIG. 1 is a schematic view of the refrigeration unit of the present invention;
FIG. 2 is a schematic view of the refrigeration unit of the present invention;
FIG. 3 is a schematic structural view of the cabinet of the present invention;
fig. 4 is a schematic structural view of the cabinet body of the invention;
fig. 5 is a schematic view of the construction of the refrigeration unit of the present invention;
FIG. 6 is a schematic view of the refrigerator of the present invention;
FIG. 7 is a sectional view of a refrigerator according to the present invention;
fig. 8 is a schematic view of a trigger tube configuration of the present invention.
Detailed Description
The technical solutions in the embodiments of the present invention will be clearly and completely described below with reference to the drawings in the embodiments of the present invention, and it is obvious that the described embodiments are only a part of the embodiments of the present invention, and not all of the embodiments. All other embodiments, which can be derived by a person skilled in the art from the embodiments given herein without making any creative effort, shall fall within the protection scope of the present invention.
A refrigerating plant for stem cell culture, refrigerating plant includes the cabinet body 1, as shown in fig. 1, fig. 2, fig. 3, fig. 4, one side of the cabinet body 1 is equipped with the refrigeration groove 12 of array distribution, is equipped with temperature detect switch 13 on arbitrary one refrigeration groove 12, and two adjacent refrigeration groove 12 notch position communicate each other.
The cabinet body 1 is provided with placing grooves 14 distributed in an array manner on the opposite sides of the refrigerating groove 12, the placing grooves 14 and the refrigerating groove 12 are distributed in a cross manner, the placing grooves 14 are located below the refrigerating groove 12, and an equipment groove 15 is arranged below the placing grooves 14.
One side of the cabinet body 1 is provided with an electric control unit 2, one side of the cabinet body 1, which is provided with a placing groove 14, is provided with a first door body 3, and the other side is provided with a second door body 4.
A placing frame 5 for placing a container for containing the stem cells is arranged in the refrigerating groove 12.
The cabinet body 1 is internally provided with a refrigerating part 6, as shown in fig. 5, the refrigerating part 6 comprises a refrigerator 61, a first communicating pipe 62, a second communicating pipe 63, a hydrogen tank 64, a hydrogen pump 65, a first electromagnetic valve 66 and a second electromagnetic valve 67 which are distributed in an array, one end of the refrigerator 61 is communicated with the first communicating pipe 62, and the other end is communicated with the second communicating pipe 63.
One end of the first communicating pipe 62 is closed, the other end is communicated with the hydrogen tank 64, and the second electromagnetic valve 67 is positioned on a pipe body between the first communicating pipe 62 and the hydrogen tank 64 to control the on-off of the first communicating pipe 62 and the hydrogen tank 64.
One end of the hydrogen pump 65 is communicated with the hydrogen tank 64, the other end is communicated with one end of the second communication pipe 63, and the other end of the second communication pipe 63 is sealed.
The first solenoid valve 66 is located on a pipe body of the hydrogen pump 65 communicating with the second communication pipe 63.
The refrigerators 61 are distributed in an S-shape, as shown in fig. 6, 7, and 8, each refrigerator 61 includes holding pipes 613 distributed in an array, adjacent holding pipes 613 are sequentially connected end to end, third communication pipes 611 are disposed at the other ends of the holding pipes 613, and each refrigerator 61 is communicated with the first communication pipe 62 through the third communication pipe 611 on one side and is communicated with the second communication pipe 63 through the third communication pipe 611 on the other side.
The two ends of the containing pipe body 613 are provided with communicating pieces 612, the communicating pieces 612 are internally provided with sealing blocks 614, trigger pipes 615 distributed in an array mode are arranged between the two sealing blocks 614, communicating holes 616 distributed in an array mode are formed in the trigger pipes 615, the communicating holes 616 are communicated with the containing pipe body 613 and the trigger pipes 615, lanthanum-nickel-tin alloy powder is contained in the containing pipe body 613, and the fineness of the lanthanum-nickel-tin alloy powder is larger than that of the communicating holes 616.
The first electromagnetic valve 66, the second electromagnetic valve 67, the hydrogen pump 65 and the temperature control switch 13 are all electrically connected with the electronic control unit 2.
During the use, place the stem cell in cold-storage tank 12, close second solenoid valve 67, first solenoid valve 66 is opened, hydrogen pump 65 works, produce the negative pressure in to refrigerator 61, make lanthanum nickel tin alloy powder take place dehydrogenation reaction, absorb the heat through the reaction of putting hydrogen of lanthanum nickel tin alloy powder, refrigerate in cold-storage tank 12, detect the temperature in cold-storage tank 12 through temperature detect switch 13, hydrogen pump 65 inhales hydrogen in hydrogen pump 65, through the rotational speed of adjusting hydrogen pump 65, the negative pressure in the control refrigerator 61, thereby realize the control of temperature.
In the description herein, references to the description of "one embodiment," "an example," "a specific example" or the like are intended to mean that a particular feature, structure, material, or characteristic described in connection with the embodiment or example is included in at least one embodiment or example of the invention. In this specification, the schematic representations of the terms used above do not necessarily refer to the same embodiment or example. Furthermore, the particular features, structures, materials, or characteristics described may be combined in any suitable manner in any one or more embodiments or examples.
The foregoing shows and describes the general principles, essential features, and advantages of the invention. It will be understood by those skilled in the art that the present invention is not limited to the embodiments described above, which are described in the specification and illustrated only to illustrate the principle of the present invention, but that various changes and modifications may be made therein without departing from the spirit and scope of the present invention, which fall within the scope of the invention as claimed.

Claims (6)

1. A refrigerating device for stem cell culture comprises a cabinet body (1), and is characterized in that refrigerating grooves (12) distributed in an array manner are formed in one side of the cabinet body (1), and a temperature control switch (13) is arranged on any refrigerating groove (12);
the refrigerator is characterized in that the cabinet body (1) is provided with placing grooves (14) distributed in an array manner on the opposite sides of the refrigerating groove (12), the placing grooves (14) and the refrigerating groove (12) are distributed in a crossed manner, and an equipment groove (15) is arranged below the placing grooves (14);
an electric control unit (2) is arranged on one side of the cabinet body (1), a first door body (3) is arranged on one side of the cabinet body (1) provided with a placing groove (14), and a second door body (4) is arranged on one side provided with a refrigerating groove (12);
a placing rack (5) for placing a container for containing stem cells is arranged in the refrigerating groove (12);
a refrigerating piece (6) is arranged in the cabinet body (1), and the refrigerating piece (6) comprises refrigerators (61), a first communicating pipe (62), a second communicating pipe (63), a hydrogen tank (64), a hydrogen pump (65), a first electromagnetic valve (66) and a second electromagnetic valve (67) which are distributed in an array manner;
one end of the refrigerator (61) is communicated with the first communicating pipe (62), and the other end of the refrigerator is communicated with the second communicating pipe (63);
one end of the first communication pipe (62) is closed, the other end of the first communication pipe is communicated with the hydrogen tank (64), and the second electromagnetic valve (67) is positioned on a pipe body between the first communication pipe (62) and the hydrogen tank (64) and controls the connection and disconnection of the first communication pipe (62) and the hydrogen tank (64);
one end of the hydrogen pump (65) is communicated with the hydrogen tank (64), the other end of the hydrogen pump is communicated with one end of the second communicating pipe (63), and the other end of the second communicating pipe (63) is sealed;
the first electromagnetic valve (66) is positioned on a pipe body of the hydrogen pump (65) communicated with the second communicating pipe (63);
the refrigerators (61) are distributed in an S shape, and the refrigerators (61) comprise containing pipes (613) distributed in an array;
both ends of splendid attire body (613) all are equipped with connecting piece (612), are equipped with sealed piece (614) in connecting piece (612), are equipped with trigger tube (615) that the array distributes between two sealed pieces (614), are equipped with intercommunicating pore (616) that the array distributes on trigger tube (615), and intercommunicating pore (616) intercommunication splendid attire body (613) and trigger tube (615), contain lanthanum nickel tin alloy powder in splendid attire body (613).
2. A refrigerator for stem cell culture according to claim 1, wherein the notches of two adjacent refrigerator chambers (12) are in communication with each other.
3. A refrigerator for stem cell culture according to claim 1, characterized in that the placement tank (14) is located below the refrigerator tank (12).
4. A refrigerator for stem cell culture according to claim 1, wherein the adjacent containing pipes (613) are connected end to end in sequence, the other ends of the containing pipes (613) are provided with third connecting pipes (611), and the refrigerator (61) is connected to the first connecting pipe (62) through the third connecting pipe (611) on one side and connected to the second connecting pipe (63) through the third connecting pipe (611) on the other side.
5. A refrigerator for stem cell culture according to claim 1, wherein the container body (613) contains lanthanum nickel tin alloy powder having a fineness larger than that of the communicating hole (616).
6. A refrigerator for stem cell culture according to claim 1, wherein the first solenoid valve (66), the second solenoid valve (67), the hydrogen pump (65) and the temperature controlled switch (13) are all electrically connected to the electronic control unit (2).
CN201911205006.1A 2019-11-29 2019-11-29 A cold storage plant for stem cell culture Active CN110887291B (en)

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Application Number Priority Date Filing Date Title
CN201911205006.1A CN110887291B (en) 2019-11-29 2019-11-29 A cold storage plant for stem cell culture

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Application Number Priority Date Filing Date Title
CN201911205006.1A CN110887291B (en) 2019-11-29 2019-11-29 A cold storage plant for stem cell culture

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CN110887291B true CN110887291B (en) 2021-03-30

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Citations (6)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US4757692A (en) * 1987-12-14 1988-07-19 Mcdonald Gordon K Embryo freezer
US4939902A (en) * 1989-10-24 1990-07-10 Retallick William B Air conditioner for an automobile
CN206284224U (en) * 2016-12-19 2017-06-30 郑州大学 A kind of stem cell refrigerated transport case
CN107228518A (en) * 2017-06-04 2017-10-03 王玉杰 Stem cell injecta transporting preservation device
CN208887201U (en) * 2018-09-28 2019-05-21 上海美致臻生物医学科技有限公司 A kind of portable multi-function stem cell transport storage device
CN208908957U (en) * 2018-09-29 2019-05-31 吉林省嘉华健康产业股份公司 A kind of stem cell refrigerating transporting apparatus

Patent Citations (6)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US4757692A (en) * 1987-12-14 1988-07-19 Mcdonald Gordon K Embryo freezer
US4939902A (en) * 1989-10-24 1990-07-10 Retallick William B Air conditioner for an automobile
CN206284224U (en) * 2016-12-19 2017-06-30 郑州大学 A kind of stem cell refrigerated transport case
CN107228518A (en) * 2017-06-04 2017-10-03 王玉杰 Stem cell injecta transporting preservation device
CN208887201U (en) * 2018-09-28 2019-05-21 上海美致臻生物医学科技有限公司 A kind of portable multi-function stem cell transport storage device
CN208908957U (en) * 2018-09-29 2019-05-31 吉林省嘉华健康产业股份公司 A kind of stem cell refrigerating transporting apparatus

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Application publication date: 20200317

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