CN110882404A

CN110882404A - Low-temperature formaldehyde sterilization indicator card and manufacturing method thereof

Info

Publication number: CN110882404A
Application number: CN201911046962.XA
Authority: CN
Inventors: 郑敏; 程希彤
Original assignee: SUZHOU LUSHUI BIOLOGICAL TECHNOLOGY Co Ltd
Current assignee: SUZHOU LUSHUI BIOLOGICAL TECHNOLOGY Co Ltd
Priority date: 2019-10-30
Filing date: 2019-10-30
Publication date: 2020-03-17

Abstract

The invention provides a low-temperature formaldehyde sterilization indicator card and a manufacturing method thereof.

Description

Low-temperature formaldehyde sterilization indicator card and manufacturing method thereof

Technical Field

The invention belongs to the field of biological sterilization indication, and particularly relates to a low-temperature formaldehyde sterilization indication card and a manufacturing method thereof.

Background

With the continuous development of medical science, more and more medical instruments are made of materials which cannot resist high temperature, so that a sterilization method cannot be a high-temperature high-pressure steam sterilization method, and only a low-temperature sterilization method can be selected. The low-temperature sterilization methods are various and correspondingly changed along with the development of times, and the methods include Ethylene Oxide (EO) sterilization, low-temperature steam formaldehyde (LT SF) sterilization, hydrogen peroxide plasma (plasma) sterilization and the like from the traditional methods of soaking and fumigating by using a chemical disinfectant. The principle of low-temperature formaldehyde sterilization (LT SF) is that aldehyde groups in formaldehyde molecules can react with amino groups, carboxyl groups, hydroxyl groups, sulfydryl groups and the like in microbial proteins and nucleic acid molecules to generate methine derivatives, so that the activity of the biomolecules is destroyed, and the microorganisms die.

In the prior art, three methods are mainly used for measuring the formaldehyde steam sterilization effect, namely a thermal sensitive thermocouple junction temperature recorder and a biochemical measuring method, but the thermal sensitive thermocouple junction temperature recorder is complicated to operate and is not suitable for popularization; the second method has higher cost and is not suitable for wide use; the third method is a chemical indicator card detection method, which is more universal than the two methods, but the chemical indicator card can absorb water under the environment with low temperature and high humidity, so that the color development of the indicator card is abnormal, and the final detection result is influenced.

Disclosure of Invention

In view of the above problems, the present invention provides a novel indicator card for low-temperature formaldehyde sterilization, which has the advantages of moisture resistance, water resistance, low cost, simple operation, reliable measurement effect, etc., and a method for manufacturing the indicator card. The specific technical scheme is as follows:

the low-temperature formaldehyde sterilization indicator card is characterized by comprising a test strip and a hydrophilic-hydrophobic bidirectional membrane, wherein the test strip is provided with a sterilization indicator area and a contrast area, and a formaldehyde sterilization indicator is coated on the sterilization indicator area; the hydrophilic and hydrophobic bidirectional membrane comprises a water absorption membrane, and one side of the water absorption membrane is coated with a layer of hydrophobic membrane; the hydrophilic and hydrophobic bidirectional membrane is pasted on the test strip and covers one side of the sterilization indication area and one side of the comparison area, wherein one side of the water absorption membrane faces the sterilization indication area and the comparison area, and one side of the hydrophobic membrane faces back to the sterilization indication area and the comparison area.

Further, the formaldehyde sterilization indicator comprises a dye and a solvent, wherein the dye is Congo red, and direct red can be selected as the dye in other embodiments; the solvent is two or more compound solutions of methanol, ethanol, isopropanol, ethyl acetate, DMF, DMSO, butanol and tetrahydrofuran.

The manufacturing method of the low-temperature formaldehyde sterilization indicator card is characterized by comprising the following steps of:

step one, preparing a test strip: brushing a formaldehyde sterilization indicator and a contrast color block on the test strip;

step two, preparing a hydrophilic and hydrophobic bidirectional membrane: selecting a polyester fiber film as water-absorbing film paper, uniformly scraping a viscous water-based amphiphobic finishing agent on the surface of one side of the polyester fiber film by using a scraper, drying and cutting into strips;

and step three, coating the edge of the surface of the hydrophilic and hydrophobic bidirectional membrane, which is not coated with the finishing agent, with glue, and adhering the coated edge to a test strip.

Further, the drying is to pre-dry for 30min at 80 ℃ and then dry for 15min at high temperature of 160 ℃.

Additional aspects and advantages of the invention will be set forth in part in the description which follows and, in part, will be obvious from the description, or may be learned by practice of the invention.

Detailed Description

The following detailed description of embodiments of the invention is intended to be illustrative, and is intended to be illustrative only.

The low-temperature formaldehyde sterilization indicator card comprises a test strip and a hydrophilic-hydrophobic bidirectional membrane, wherein the test strip is provided with a sterilization indicator area and a contrast area, and a formaldehyde sterilization indicator is coated on the sterilization indicator area; the hydrophilic and hydrophobic bidirectional membrane comprises a water absorption membrane, and one side of the water absorption membrane is coated with a layer of hydrophobic membrane; the hydrophilic and hydrophobic bidirectional membrane is pasted on the test strip and covers one side of the sterilization indication area and one side of the comparison area, wherein one side of the water absorption membrane faces the sterilization indication area and the comparison area, and one side of the hydrophobic membrane faces back to the sterilization indication area and the comparison area.

The surface of the hydrophilic and hydrophobic bidirectional membrane has the characteristics of transparency, softness, hydrophobicity, water resistance and air permeability. The formaldehyde sterilization indicator on the sterilization indicating area generates color change reaction through contact with formaldehyde steam, and the final sterilization effect can be judged through comparison with the contrast color block on the contrast area.

Further, the formaldehyde sterilization indicator comprises a dye and a solvent, wherein the dye is Congo red; the solvent is two or more than two compound solutions of methanol, ethanol, isopropanol, ethyl acetate, DMF, DMSO, butanol and tetrahydrofuran.

step two, preparing a hydrophilic and hydrophobic bidirectional membrane: selecting a polyester fiber film as water-absorbing film paper, uniformly scraping a viscous water-based amphiphobic finishing agent on the surface of one side of the polyester fiber film by using a scraper, drying and cutting into strips; in other embodiments, the cellulose acetate film can be selected as the water-absorbing film paper;

wherein the finishing agent comprises the following main components: after the finishing agent is coated on one side, the finishing agent is pre-dried for 30min at the temperature of 80 ℃, and then is dried for 15min at the high temperature of 160 ℃, and the hydrophilic and hydrophobic bidirectional membrane is prepared after the single-side treatment, the back part, namely the surface without the finishing agent, still has the hydrophilic effect, the front surface, namely the surface coated with the finishing agent, has the hydrophobic and water-blocking effects, and the whole body also has the ventilation effect, thereby being beneficial to the combination and color development of the formaldehyde steam which penetrates through the hydrophilic and hydrophobic bidirectional membrane and the formaldehyde sterilization indicator on the test paper strip.

Preparing a hydrophilic-hydrophobic bidirectional membrane, preferably a polyester fiber membrane of 24cm multiplied by 24cm, coating a finishing agent, drying, and cutting into strips with proper sizes so as to be separately stuck on the test paper strips.

Utilize the low temperature formaldehyde sterilization indicator card in this embodiment to detect formaldehyde sterilization effect convenient, the degree of accuracy is high, simultaneously because the setting of hydrophilic and hydrophobic two-way membrane on the test paper strip makes low temperature formaldehyde sterilization indicator card itself not receive low temperature, moist detection environment's restriction, the demonstration sterilization effect that equally can be outstanding.

While specific embodiments of the invention have been described in detail with reference to exemplary embodiments thereof, it should be understood that numerous other modifications and embodiments can be devised by those skilled in the art that will fall within the spirit and scope of the principles of this invention.

Claims

1. The low-temperature formaldehyde sterilization indicator card is characterized by comprising a test strip and a hydrophilic-hydrophobic bidirectional membrane, wherein the test strip is provided with a sterilization indicator area and a contrast area, and a formaldehyde sterilization indicator is coated on the sterilization indicator area; the hydrophilic and hydrophobic bidirectional membrane comprises a water absorption membrane, and one side of the water absorption membrane is coated with a layer of hydrophobic membrane; the hydrophilic and hydrophobic bidirectional membrane is pasted on the test strip and covers one side of the sterilization indication area and one side of the comparison area, wherein one side of the water absorption membrane faces the sterilization indication area and the comparison area, and one side of the hydrophobic membrane faces back to the sterilization indication area and the comparison area.

2. The low temperature formaldehyde sterilization indicator card of claim 1, wherein the formaldehyde sterilization indicator comprises a dye and a solvent, the dye being congo red; the solvent is two or more than two compound solutions of methanol, ethanol, isopropanol, ethyl acetate, DMF, DMSO, butanol and tetrahydrofuran.

3. A method for manufacturing a low-temperature formaldehyde sterilization indicator card according to any one of claims 1-2, comprising the steps of:

4. The method for manufacturing the low-temperature formaldehyde sterilization indicator card according to claim 3, wherein the drying is performed by pre-drying at 80 ℃ for 30min and then drying at a high temperature of 160 ℃ for 15 min.