CN1987461B - Dry chemical quick detecting reagent strip for glutamic-pyruvic transaminase and its producing method - Google Patents
Dry chemical quick detecting reagent strip for glutamic-pyruvic transaminase and its producing method Download PDFInfo
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- CN1987461B CN1987461B CN2006101614307A CN200610161430A CN1987461B CN 1987461 B CN1987461 B CN 1987461B CN 2006101614307 A CN2006101614307 A CN 2006101614307A CN 200610161430 A CN200610161430 A CN 200610161430A CN 1987461 B CN1987461 B CN 1987461B
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Abstract
Purpose of the invention is to provide a reagent bar capable of half quantitative/ quantitative detecting whether content of glutamic-pyruvic transaminase (GPT) inside human body is normal or not rapidly, and relevant fabricating method. The reagent bar includes PVC hard strip for supporting reaction. The hard strip possesses carrier film of adsorbing L-alanine, pyruvate oxidase, and alpha-ketoglutarate. Belonging to fast test in micro scale, the disclosed product is one-off consumption material for testing. Range for detecting GPT is 0-1000U/L.
Description
Technical field
The present invention relates to a kind of quick detection reagent bar and manufacture method thereof, particularly relate to a kind of reagent strip and manufacture method that detects glutamic-pyruvic transaminase.
Background technology
Reagent strip is mainly used in GPT content in the human body is carried out the fast qualitative detection, understands the present hepar damnification degree of human body and judges result of treatment, also can carry out conventional inspection to detect liver function to healthy population.Reagent strip is in the exploitation maturity stage at present, utilizes this reagent strip and supporting colorimetric card can directly carry out coherent detection.The problem that this reagent strip exists mainly be difficult to testing result is carried out quantitative accurately.
Summary of the invention
The object of the present invention is to provide a kind of glutamic-pyruvic transaminase dry chemical method fast measuring reagent strip and manufacture method thereof, so that the also direct content of the interior GPT of human body of the instrument that does not rely on specialty clinically, relatively accurate result can be carried out and obtain to the experimental implementation that feasible script must be undertaken by the professional person in hospital equally also apace by the layman at other Anywhere.
According to technology Fang An provided by the invention; glutamic-pyruvic transaminase dry chemical method fast measuring reagent strip includes the hard lath of certain degree of hardness; on lath, be stained with the carrier film of having adsorbed reactive material; reactive material comprises α-ketoglutaric acid; L-alanine and pyruvate oxidase and hydrogen peroxidase; it is characterized in that: on lath, adhere to the carrier film that one deck has adsorbed α-ketoglutaric acid; adhere to the carrier film that one deck has adsorbed pyruvate oxidase and catalatic carrier film and adsorbed L-alanine on the carrier film of α-ketoglutaric acid more respectively adsorbing; on the carrier film of having adsorbed L-alanine, also be stained with a netted pre-filtrating equipment that is used for balanced sample; on outermost carrier film, adhere to the layer of transparent diaphragm, with protection entire reaction device.
Having adsorbed pyruvate oxidase and catalatic carrier film is spaced from each other with the carrier film of having adsorbed L-alanine.Described reagent strip also comprises corresponding colorimetric card.Carrier film is a kind of commercial tunica fibrosa; Reactive material also comprises chromogenic substrate.
Produce the method for glutamic-pyruvic transaminase dry chemical method fast measuring reagent strip, it is characterized in that,
A, preparation are adsorbed with the carrier film of L-alanine: with L-alanine solution impregnation carrier film of 250～450mM, the carrier film after will soaking into is again at room temperature dried;
B, preparation are adsorbed with pyruvate oxidase and catalatic carrier film: with the hydrogen peroxidase solution impregnation carrier film of pyruvate oxidase and the 40～120U/ml of 40～120U/ml, the carrier film after will soaking into is again at room temperature dried;
C, preparation are adsorbed with the carrier film of α-ketoglutaric acid: with α-ketoglutaric acid solution impregnation carrier film of 15～50mM, the carrier film after will soaking into is again at room temperature dried;
D, treat above-mentioned carrier film bone dry after; according to reaction sequence; the carrier film that will be adsorbed with α-ketoglutaric acid earlier sticks on the hard lath; adhere to the carrier film of having adsorbed pyruvate oxidase and catalatic carrier film and having adsorbed L-alanine being adsorbed with on the carrier film of α-ketoglutaric acid again; adhering protective film on the outermost layer carrier film obtains this product then.
MM as herein described refers to mM, is concentration unit; U/ml refers to every milliliter of what active units; U/L refers to every liter of how many active units.
Advantage of the present invention is: utilize the principle of dry chemical dexterously, and creatively with the immobilization in advance of the main participation material in the entire reaction course, make the process that detects GPT shorten greatly and simplify.On-the-spot only the detection needs 5 minutes, and in testing process if qualitative or half-quantitative detection, without any need for utility appliance, this makes field quick detection or family's self check become possibility.
Description of drawings
Fig. 1 is a synoptic diagram of the present invention.
Embodiment
As shown in the figure: on the PVC of certain degree of hardness hard lath 6, adhere to the carrier film of having adsorbed reactive material; reactive material comprises α-ketoglutaric acid; L-alanine and pyruvate oxidase and hydrogen peroxidase; on lath 6, adhere to the carrier film 5 that one deck has adsorbed α-ketoglutaric acid; on the carrier film 5 of adsorbing α-ketoglutaric acid, adhere to one deck more respectively and adsorbed pyruvate oxidase and catalatic carrier film 1 and the carrier film 4 of having adsorbed L-alanine; on the carrier film 4 of having adsorbed L-alanine, also be stained with a netted pre-filtrating equipment 3 that is used for balanced sample; on outermost carrier film, adhere to layer of transparent diaphragm 2, with protection entire reaction device.Having adsorbed pyruvate oxidase and catalatic carrier film 1 is spaced from each other with the carrier film 4 of having adsorbed L-alanine.Described reagent strip also comprises corresponding colorimetric card.Carrier film is a kind of commercial tunica fibrosa; Reactive material also comprises chromogenic substrate.
Produce the method for glutamic-pyruvic transaminase dry chemical method fast measuring reagent strip, it is characterized in that,
A, preparation are adsorbed with the carrier film 4 of L-alanine: (250～450mM) soak into carrier film, dry under the room temperature with L-alanine solution;
B, preparation are adsorbed with pyruvate oxidase and catalatic carrier film 1: with pyruvate oxidase (40～120U/ml) with the hydrogen peroxide enzyme solutions (40～120U/ml) infiltration carrier film are dried under the room temperature;
C, preparation are adsorbed with the carrier film 5 of α-ketoglutaric acid: (15～50mM) soak into carrier film, dry under the room temperature with α-ketoglutaric acid solution;
D, treat above-mentioned carrier film bone dry after; according to reaction sequence; the carrier film 5 that will be adsorbed with α-ketoglutaric acid earlier sticks on the hard lath 6; on the carrier film 5 that is adsorbed with α-ketoglutaric acid, adhere to again and adsorbed pyruvate oxidase and catalatic carrier film 1 and the carrier film 4 of having adsorbed L-alanine; adhering protective film 2 on the outermost layer carrier film obtains this product then.
The reagent strip made from method of the present invention, because of various materials all in advance by immobilization, so under rated condition, can preserve 18 months, be fit to extensive the recommendation and use.
Operating process is as follows:
(1), with reagent strip as required quantity take out, stand-by;
(2), adopt tip blood/plasma of 32U/L detected person, directly drip balanced online in the reagent strip upper end;
(3), react after 5～10 minutes the color behind the hyaline membrane of observation lower end;
(4), the result judges: tangible blueness promptly positive (surpassing 40U/L) occurs; Colourless or light blue promptly negative (being lower than 40U/L).
Carrier film of the present invention promptly refers to be adsorbed with the film of reactive material, general disposal route is about to reactive material and melts in solution, adsorb this solution with film then, be drying to obtain carrier film, in use, because answering of solution melted effect, the reactive material that was adsorbed is originally discharged again participates in reaction.
Claims (1)
1. glutamic-pyruvic transaminase dry chemical method fast measuring reagent strip, include the hard lath (6) of certain degree of hardness, on lath (6), be stained with the carrier film of having adsorbed reactive material, reactive material comprises α-Tong Wuersuan, L-alanine and pyruvate oxidase and hydrogen peroxidase, it is characterized in that: go up at lath (6) and adhere to the carrier film (5) that one deck has adsorbed α-Tong Wuersuan, adhere to the carrier film (4) that one deck has adsorbed pyruvate oxidase and catalatic carrier film (1) and adsorbed the L-alanine on the carrier film of α-Tong Wuersuan (5) more respectively adsorbing, also be stained with a netted pre-filtrating equipment (3) that is used for balanced sample on the carrier film (4) of L-alanine having adsorbed, on outermost carrier film, adhere to layer of transparent diaphragm (2), with protection entire reaction device;
The carrier film (4) of having adsorbed pyruvate oxidase and catalatic carrier film (1) and having adsorbed the L-alanine is spaced from each other on the carrier film of having adsorbed α-Tong Wuersuan (5);
Described reagent strip also comprises corresponding colorimetric card;
Carrier film is a kind of commercial tunica fibrosa; Reactive material also comprises the recessive pigment of chromogenic substrate.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN2006101614307A CN1987461B (en) | 2006-12-12 | 2006-12-12 | Dry chemical quick detecting reagent strip for glutamic-pyruvic transaminase and its producing method |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN2006101614307A CN1987461B (en) | 2006-12-12 | 2006-12-12 | Dry chemical quick detecting reagent strip for glutamic-pyruvic transaminase and its producing method |
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| Publication Number | Publication Date |
|---|---|
| CN1987461A CN1987461A (en) | 2007-06-27 |
| CN1987461B true CN1987461B (en) | 2011-09-28 |
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| CN2006101614307A Active CN1987461B (en) | 2006-12-12 | 2006-12-12 | Dry chemical quick detecting reagent strip for glutamic-pyruvic transaminase and its producing method |
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Families Citing this family (4)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN101105491B (en) * | 2007-08-09 | 2011-12-28 | 上海科华生物工程股份有限公司 | Dry chemical test paper for quantitatively determining human alanine aminotransferase |
| CN101216490B (en) * | 2008-01-16 | 2012-05-30 | 盛青松 | Dry chemical method TORCH detection reagent kit and method of manufacture |
| CN102288747B (en) * | 2011-08-19 | 2013-11-27 | 长沙三诺生物传感技术股份有限公司 | Interference-removing dry chemical quantitative test strip, alanine and aspartate aminotransferase quantitative test strip and test method thereof |
| CN109900689B (en) * | 2019-03-28 | 2021-12-10 | 复星诊断科技(长沙)有限公司 | Anti-interference membrane and liver function combined test strip |
Citations (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN1237706A (en) * | 1998-05-30 | 1999-12-08 | 肖佳民 | Reagent strip for visualizing breast milk |
| CN1271860A (en) * | 1999-04-23 | 2000-11-01 | 郑州博赛生物技术研究所 | Test strip for detecting more antigens of typhoid bacillus |
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2006
- 2006-12-12 CN CN2006101614307A patent/CN1987461B/en active Active
Patent Citations (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN1237706A (en) * | 1998-05-30 | 1999-12-08 | 肖佳民 | Reagent strip for visualizing breast milk |
| CN1271860A (en) * | 1999-04-23 | 2000-11-01 | 郑州博赛生物技术研究所 | Test strip for detecting more antigens of typhoid bacillus |
Non-Patent Citations (1)
| Title |
|---|
| 马心林.干化学法测定谷丙转氨酶的方法学研究.《齐鲁医学检验》.2004,第15卷(第6期),23-25. * |
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Assignee: Wuxi Shenrui Bio-Pharmaceuticals Co., Ltd. Assignor: Sheng Qingsong Contract record no.: 2012320000704 Denomination of invention: Dry chemical quick detecting reagent strip for glutamic-pyruvic transaminase and its producing method Granted publication date: 20110928 License type: Exclusive License Open date: 20070627 Record date: 20120604 |
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Effective date of registration: 20201224 Address after: 5 / F, c30303, C5, area C, Wuxi Huishan Life Science Park, 1699 Huishan Avenue, Huishan District, Wuxi City, Jiangsu Province Patentee after: WUXI SHENRUI BIO-PHARMACEUTICALS Co.,Ltd. Address before: 214058 Shen Rui Biological Products Co., Ltd., Huang Xiang hospital, 118 Fengxiang Road, Beitang District, Wuxi, Jiangsu Patentee before: Sheng Qingsong |