CN110876222A - Cold plasma generator for mutation breeding - Google Patents

Cold plasma generator for mutation breeding Download PDF

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CN110876222A
CN110876222A CN201811009063.8A CN201811009063A CN110876222A CN 110876222 A CN110876222 A CN 110876222A CN 201811009063 A CN201811009063 A CN 201811009063A CN 110876222 A CN110876222 A CN 110876222A
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electrode
radio frequency
frequency electrode
ground electrode
radio
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王立言
段保峰
张金强
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WUXI TMAXTREE BIOTECHNOLOGY Co Ltd
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WUXI TMAXTREE BIOTECHNOLOGY Co Ltd
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    • H—ELECTRICITY
    • H05—ELECTRIC TECHNIQUES NOT OTHERWISE PROVIDED FOR
    • H05H—PLASMA TECHNIQUE; PRODUCTION OF ACCELERATED ELECTRICALLY-CHARGED PARTICLES OR OF NEUTRONS; PRODUCTION OR ACCELERATION OF NEUTRAL MOLECULAR OR ATOMIC BEAMS
    • H05H1/00—Generating plasma; Handling plasma
    • H05H1/02—Arrangements for confining plasma by electric or magnetic fields; Arrangements for heating plasma
    • A—HUMAN NECESSITIES
    • A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
    • A01H—NEW PLANTS OR NON-TRANSGENIC PROCESSES FOR OBTAINING THEM; PLANT REPRODUCTION BY TISSUE CULTURE TECHNIQUES
    • A01H1/00—Processes for modifying genotypes ; Plants characterised by associated natural traits
    • A01H1/06—Processes for producing mutations, e.g. treatment with chemicals or with radiation
    • A—HUMAN NECESSITIES
    • A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
    • A01K—ANIMAL HUSBANDRY; AVICULTURE; APICULTURE; PISCICULTURE; FISHING; REARING OR BREEDING ANIMALS, NOT OTHERWISE PROVIDED FOR; NEW BREEDS OF ANIMALS
    • A01K67/00—Rearing or breeding animals, not otherwise provided for; New or modified breeds of animals
    • A01K67/027—New or modified breeds of vertebrates
    • A01K67/0275—Genetically modified vertebrates, e.g. transgenic
    • A—HUMAN NECESSITIES
    • A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
    • A01K—ANIMAL HUSBANDRY; AVICULTURE; APICULTURE; PISCICULTURE; FISHING; REARING OR BREEDING ANIMALS, NOT OTHERWISE PROVIDED FOR; NEW BREEDS OF ANIMALS
    • A01K67/00—Rearing or breeding animals, not otherwise provided for; New or modified breeds of animals
    • A01K67/033—Rearing or breeding invertebrates; New breeds of invertebrates
    • A01K67/0333—Genetically modified invertebrates, e.g. transgenic, polyploid
    • H—ELECTRICITY
    • H05—ELECTRIC TECHNIQUES NOT OTHERWISE PROVIDED FOR
    • H05H—PLASMA TECHNIQUE; PRODUCTION OF ACCELERATED ELECTRICALLY-CHARGED PARTICLES OR OF NEUTRONS; PRODUCTION OR ACCELERATION OF NEUTRAL MOLECULAR OR ATOMIC BEAMS
    • H05H1/00—Generating plasma; Handling plasma
    • H05H1/24—Generating plasma
    • H—ELECTRICITY
    • H05—ELECTRIC TECHNIQUES NOT OTHERWISE PROVIDED FOR
    • H05H—PLASMA TECHNIQUE; PRODUCTION OF ACCELERATED ELECTRICALLY-CHARGED PARTICLES OR OF NEUTRONS; PRODUCTION OR ACCELERATION OF NEUTRAL MOLECULAR OR ATOMIC BEAMS
    • H05H1/00—Generating plasma; Handling plasma
    • H05H1/24—Generating plasma
    • H05H1/2406—Generating plasma using dielectric barrier discharges, i.e. with a dielectric interposed between the electrodes

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  • Engineering & Computer Science (AREA)
  • Physics & Mathematics (AREA)
  • Environmental Sciences (AREA)
  • Plasma & Fusion (AREA)
  • Spectroscopy & Molecular Physics (AREA)
  • Health & Medical Sciences (AREA)
  • Biotechnology (AREA)
  • Animal Husbandry (AREA)
  • Biodiversity & Conservation Biology (AREA)
  • Zoology (AREA)
  • Animal Behavior & Ethology (AREA)
  • General Health & Medical Sciences (AREA)
  • Genetics & Genomics (AREA)
  • Veterinary Medicine (AREA)
  • Optics & Photonics (AREA)
  • Molecular Biology (AREA)
  • Botany (AREA)
  • Developmental Biology & Embryology (AREA)
  • Plasma Technology (AREA)

Abstract

The invention relates to a cold plasma generator for mutation breeding, which comprises an upper cover plate, a radio frequency electrode, a lower cover plate and a ground electrode, and is characterized in that the radio frequency electrode is connected with a radio frequency power supply, the ground electrode is connected with a ground wire of the radio frequency power supply, the upper cover plate covers the radio frequency electrode and forms a gas chamber with the radio frequency electrode, an air inlet channel is arranged on the upper cover plate, the radio frequency electrode is provided with a protruded parallel lath, the ground electrode is provided with a narrow groove matched with the lath of the radio frequency electrode, and the parallel lath of the radio frequency electrode can penetrate through the narrow groove of a lower partition plate and is inserted between the narrow grooves of the ground electrode in parallel; a plasma discharge chamber is formed between the parallel laths of the radio-frequency electrode and the narrow groove of the ground electrode, and cooling circulation channels are arranged in the radio-frequency electrode and the ground electrode, so that long-time temperature stable plasma is generated in the biological mutation breeding process, particularly in the animal and plant mutation process. The invention effectively improves the uniformity of the generated plasma, and the temperature of the plasma is stable and controllable.

Description

Cold plasma generator for mutation breeding
Technical Field
The invention belongs to the technical field of plasmas, and particularly relates to a cold plasma generator for mutation breeding.
Background
Plasma is a fourth state of matter different from solid, liquid and gas. Different excitation modes and generator configurations can produce plasmas in different thermodynamic states. In recent years, atmospheric pressure non-equilibrium plasma technology has attracted more and more attention in various fields due to the elimination of complex, expensive vacuum systems. The atmospheric pressure non-equilibrium plasma can be generated by adopting plasma generators with different electrode structures (such as a dielectric barrier structure, a needle-plate structure, a bare electrode structure and the like) and adopting power supplies with different frequencies (microwave, radio frequency, pulse, direct current and the like) for driving. The atmospheric pressure non-equilibrium plasma generation mode commonly used in the field of mutation breeding mainly comprises two modes of atmospheric pressure dielectric barrier discharge and atmospheric pressure radio frequency glow discharge.
The active particles in the plasma act on microorganisms, can cause the change of gene sequences and metabolic networks thereof, and are widely applied to the breeding of the microorganisms. In animal and plant breeding, because the samples needed to be adopted are large and many, and the plasma jet flow needed at a time is large, the existing plasma technology is difficult to generate large-area uniform plasmas with biological activity. In order to promote the application of plasma breeding to animal and plant breeding, it is essential to develop a plasma generator suitable for animal and plant breeding.
Patent document 1 discloses in 2002 a new electrode for an atmospheric pressure plasma emission device including a first electrode and a second electrode and a method of using the same. The second electrode is porous, i.e. configured to allow a plasma discharge to pass through it. Accordingly, the plasma is received at one side of the second electrode and emitted from the opposite plasma exit side thereof. Various configurations of the second electrode are possible as long as the plasma discharge is allowed to pass through it. For example, the second electrode may be a stack of multiple layers of insulating material with at least one conductive layer sandwiched therebetween. A plurality of apertures are defined through the stack and a media sleeve is inserted into the apertures and retained therein. The plasma reactor may be located close to or in direct contact with the surface of the object being treated as the plasma passes through the second electrode. Although this patent can generate a large amount of plasma, which solves the problem of severely limiting the size of the object to be treated, the plasma temperature cannot be effectively controlled and uniform plasma is not easily generated, which is not favorable for the application of animal and plant breeding.
Patent document 2 discloses in 2014 an atmospheric pressure discharge cold plasma generator including an upper partition plate, a radio frequency electrode, a lower partition plate and a ground electrode; the radio frequency electrode is connected with a radio frequency power supply, and the ground electrode is connected with a ground wire of the radio frequency power supply; the upper baffle plate covers the radio-frequency electrode. The radio-frequency electrode is provided with protruded parallel laths, the ground electrode and the lower clapboard are provided with narrow grooves matched with the laths of the radio-frequency electrode, and the parallel laths of the radio-frequency electrode can penetrate through the narrow grooves of the lower clapboard and are parallelly inserted between the narrow grooves of the ground electrode or penetrate through the narrow grooves of the ground electrode and then are parallelly inserted between the parallel laths of the ground electrode; forming a plasma discharge chamber between the parallel laths of the radio-frequency electrode and the narrow groove of the ground electrode or between the narrow groove and the parallel laths; the lower partition board is internally provided with criss-cross gas channels, so that the working gas can be uniformly distributed to each discharge chamber, and large-volume uniform plasma jet flow is generated under normal pressure by applying voltage. The invention solves the problem of plasma uniformity, and the plasma temperature is not easy to be effectively controlled.
Patent document 3 discloses in 2015 a simple atmospheric pressure cold plasma generator including a radio frequency electrode, a partition plate, and a ground electrode, thereby constituting a flat-plate type atmospheric pressure discharge cold plasma generator. The radio frequency electrode is provided with a transverse and longitudinal crossed gas channel structure, working gas can be effectively and uniformly distributed to the discharge chamber, the radio frequency electrode and the ground electrode are separated by the partition plate, the discharge distance between the electrodes is ensured, the ground electrode is connected with a radio frequency power supply ground wire, the lower part of the radio frequency electrode is provided with a convex structure, and the surface of the convex structure is provided with grooves which are regularly arranged and used as plasma outlets. The radio-frequency electrode is provided with criss-cross gas channels, and working gas is uniformly distributed in the discharge chamber, so that the process difficulty of the radio-frequency electrode is increased by the structure; when the generator structure is larger, air is introduced from outside to inside in sequence, and the uniformity has relativity; in addition, in animal and plant breeding, seeds or fertilized egg cells are generally treated for at least several minutes, the treatment time is long, the electrodes can generate heat, the temperature of working gas is easy to increase by arranging the air inlet channel in the radio-frequency electrode, and even if a cooling system is arranged in the ground electrode, large-area cold plasma is not easy to generate under a long-time working state.
Patent document 4 discloses in 2017 an array-type large-area cold plasma generator, which includes: the inner electrode coaxial structure comprises an inner electrode, an insulating capillary tube which completely wraps the lower end head and the outside of the inner electrode, and an insulating tube which is coaxially sleeved outside the inner electrode and is flush with the lower end of the inner electrode; the outer electrode is wrapped outside the insulating tube and has the height smaller than that of the insulating tube; the upper valve cover and the lower valve cover are respectively sleeved on the upper end opening and the lower end opening of the insulating pipe and used for fixing the axial position of the insulating pipe; the air guide pipe is positioned at the upper part of the upper valve cover and sleeved outside the inner electrode and the insulating capillary; a pipe sleeve sleeved outside the elements and playing a role in containing and protecting; and an upper cover provided at an upper end of the tube housing to allow the inner electrode and the insulating capillary to protrude. Although the invention can generate large-area plasma, the invention is of a parallel array coaxial discharge structure, and gas is only fed from one end, so that uniform plasma is not easy to generate, and the temperature of the plasma is not easy to be effectively controlled.
Patent document 1: CN1552082A
Patent document 2: CN105722294A
Patent document 3: 205071427U
Patent document 4: CN 10509298A.
Disclosure of Invention
In order to solve the problems, the invention provides a cold plasma generator for mutation breeding, which utilizes a radio frequency electrode and a ground electrode with a plate structure to generate large-volume plasma, utilizes a cooling circulation channel to effectively control the temperature of the generated plasma, and ensures the uniformity of the plasma through a gas chamber, particularly a uniform flow system. The generator can stably work under atmospheric pressure to generate a large amount of uniform cold plasmas, and can be used for mutation breeding treatment of animal and plant samples.
In order to achieve the purpose, the invention provides a cold plasma generator for mutation breeding, which comprises an upper cover plate, a radio-frequency electrode, a lower cover plate and a ground electrode, wherein the radio-frequency electrode is connected with a radio-frequency power supply, the ground electrode is connected with a ground wire of the radio-frequency power supply, the upper cover plate covers the radio-frequency electrode and forms a gas chamber with the radio-frequency electrode, an air inlet channel is arranged on the upper cover plate, the radio-frequency electrode is provided with a protruded parallel lath, the ground electrode is provided with a narrow groove matched with the lath of the radio-frequency electrode, and the parallel lath of the radio-frequency electrode can penetrate through the narrow groove of a lower partition plate and is inserted between; a plasma discharge chamber is formed between the parallel laths of the radio-frequency electrode and the narrow groove of the ground electrode, and cooling circulation channels are arranged in the radio-frequency electrode and the ground electrode, wherein the cooling channels of the radio-frequency electrode and the ground electrode form a closed-loop cooling circulation channel through a connecting pipeline, so that long-time temperature stable plasma is generated in the biological mutation breeding process, particularly in the animal and plant mutation process.
The air inlet mode of the air inlet channel is controlled by the air flow controller, the air inlet flow can be quantitatively controlled, and the plasma jet can be stably emitted in the biological mutation breeding process, particularly in the animal and plant mutation process by matching and adjusting the power set on the radio frequency power supply. The working gas entering the air inlet channel of the cold plasma generator for mutation breeding comprises inert gases such as helium, argon and the like, and can also comprise nitrogen, air and the like, and helium is preferred.
The average temperature of the plasma with stable temperature is 36-42 ℃, and the standard deviation is within 2 ℃. The temperature of the stable-temperature plasma is kept stable after working for 15-20 min and is stabilized at 36-42 ℃, and the temperature of the stable-temperature plasma is gradually increased to be not more than 36-42 ℃ before 15-20 min.
And the cooling circulation in the radio frequency electrode and the ground electrode adopts water cooling circulation or air cooling circulation. And in the water cooling circulation or the gas cooling circulation, cooling water or gas is driven by a cooling circulator to enter through an inlet, pass through the radio-frequency electrode, the cooling circulation channel and the ground electrode, and then flow back into the cooling circulator through an outlet. The water cooling circulation or the gas cooling circulation can also be realized by that the cooling circulator drives water or gas to enter through the ground electrode inlet, pass through the ground electrode, the cooling circulation channel and the radio frequency electrode and then circulate back to the cooling circulator through the radio frequency electrode outlet. Wherein the cooling circulator can control the temperature and flow rate of the cooling water or cooling gas entering the plasma, thereby further controlling the temperature of the plasma to be 36-42 ℃.
The cold plasma generator for mutation breeding is characterized in that a uniform flow system is arranged in a gas chamber, namely the uniform flow system comprises a uniform flow chamber and a uniform flow film, and the uniform flow chamber and the uniform flow film are arranged between an upper cover plate and a radio frequency electrode.
The uniform flow film in the cold plasma generator for mutation breeding at least comprises 1 uniform flow chamber which is divided into more than 2 uniform flow spaces, preferably 2 uniform flow films, and 3 uniform flow spaces. The uniform flow film is made of high polymer materials, such as environment-friendly polyester materials and the like. The mesh diameter of the uniform flow film is 0.05-0.6 mm, preferably 0.07-0.1 mm.
In the cold plasma generator for mutation breeding, the gap of a plasma discharge chamber formed between the parallel laths of the radio-frequency electrode and the narrow groove of the ground electrode is 0.5-2.5 mm, and the effective discharge height of the parallel laths of the radio-frequency electrode and the ground electrode is 1-50 mm.
In the cold plasma generator for mutation breeding, the parallel laths of the radio-frequency electrode can penetrate through the narrow grooves of the lower partition plate and parallelly penetrate between the narrow grooves of the ground electrode to form a plasma irradiation area, and the area of the irradiation area can reach 4500-6000 square millimeters.
Furthermore, parallel laths of the radio-frequency electrodes can penetrate through the narrow grooves of the lower partition plate and are parallelly inserted between the narrow grooves of the ground electrodes, the radio-frequency electrodes and the ground electrodes are arrayed, a large-area plasma irradiation area is formed at a generated plasma jet outlet, and the area of the irradiation area can reach 5900-6000 square millimeters.
The cold plasma generator for mutation breeding is characterized in that the power of the radio frequency source is 13.56MHZ, and the maximum power is 500W.
Further, helium with the purity of 99.999 percent is used as a working gas source, the gas flow is 15-20 SLM, when the power of a radio frequency power supply is 300-500W, the temperature of cooling water set by a cooling circulator is 6-10 ℃, and the average temperature of plasma is 37-40 DEG CoCAnd the temperature of the generated plasma is kept stable after 20min of operation.
The cold plasma generator for mutation breeding further comprises a bottom plate, wherein the bottom plate is arranged below the ground electrode and used for fixing the radio-frequency electrode and the ground electrode.
The invention has the following technical effects:
according to the cold plasma generator for mutation breeding, disclosed by the invention, the utilization of vacuum equipment is avoided, large-area uniform plasma jet with controllable temperature can be generated under atmospheric pressure, the plasma jet with the temperature controlled at 36-42 ℃ is ejected, the plasma generation efficiency is greatly improved, the equipment is simple in structure and easy to process, and the breeding application in the animal field can be realized.
Drawings
FIG. 1 is a perspective view of a cold plasma generator for mutation breeding according to the present invention;
FIG. 2 is a sectional view of one embodiment of a cold plasma generator for mutation breeding according to the present invention;
FIG. 3 shows the flow direction of a cooling channel of a cold plasma generator ground electrode for mutation breeding according to the present invention.
FIG. 4 is a schematic view of a plasma jet surface generated by a cold plasma generator for mutation breeding according to the present invention.
Description of the symbols:
1, an upper cover plate; 2, a radio frequency electrode; 3, a lower cover plate; 4, a ground electrode; 5 an intake passage;
6 cooling the circulating channel; 6-1 cooling the pipeline; 6-2, connecting the radio-frequency electrode with a ground electrode;
7, flow homogenizing film; 8, a flow homogenizing chamber; 9 a bottom plate.
Detailed Description
Specific embodiments of the present invention will be described in more detail below with reference to the accompanying drawings. While specific embodiments of the invention are shown in the drawings, it should be understood that the invention may be embodied in various forms and should not be construed as limited to the embodiments set forth herein. Rather, these embodiments are provided so that this disclosure will be thorough and complete, and will fully convey the scope of the invention to those skilled in the art.
It should be noted that certain terms are used throughout the description and claims to refer to particular components. As one skilled in the art will appreciate, various names may be used to refer to a component. This specification and claims do not intend to distinguish between components that differ in name but not function. In the following description and in the claims, the terms "include" and "comprise" are used in an open-ended fashion, and thus should be interpreted to mean "include, but not limited to. The description which follows is a preferred embodiment of the invention, but is made for the purpose of illustrating the general principles of the invention and not for the purpose of limiting the scope of the invention. The scope of the present invention is defined by the appended claims.
For the purpose of facilitating an understanding of the embodiments of the present invention, the following description will be made in terms of several specific embodiments with reference to the accompanying drawings, and the drawings are not intended to limit the embodiments of the present invention.
The invention relates to a cold plasma generator for mutation breeding, which comprises an upper cover plate, a radio frequency electrode, a lower cover plate and a ground electrode, wherein the radio frequency electrode is connected with a radio frequency power supply, the ground electrode is connected with a ground wire of the radio frequency power supply, the upper cover plate covers the radio frequency electrode and forms a gas cavity with the radio frequency electrode, a gas inlet channel is arranged on the upper cover plate, the radio frequency electrode is provided with a protruded parallel lath, the ground electrode is provided with a narrow groove matched with the lath of the radio frequency electrode, and the parallel lath of the radio frequency electrode can penetrate through the narrow groove of the lower partition plate and is inserted between the narrow grooves of the ground electrode in parallel; a plasma discharge chamber is formed between the parallel laths of the radio-frequency electrode and the narrow groove of the ground electrode, and cooling circulation channels are arranged in the radio-frequency electrode and the ground electrode, wherein the cooling channels of the radio-frequency electrode and the ground electrode form a closed-loop cooling circulation channel through a connecting pipeline, so that long-time temperature stable plasma is generated in the biological mutation breeding process, particularly in the animal and plant mutation process.
In an embodiment of the present invention, a cooling cycle inside the rf electrode and the ground electrode is a water cooling cycle or an air cooling cycle. The average temperature of the generated plasma jet is 36-42 ℃, the temperature of the generated plasma is kept stable after working for 15-20 min, and the standard deviation is within 2 ℃.
In a specific embodiment of the invention, the cooling cycle adopts a water cooling cycle or an air cooling cycle specific process: the cooling gas is driven by the cooling circulator to enter through the inlet, pass through the radio-frequency electrode, the cooling circulation channel and the ground electrode, and then flow back into the cooling circulator through the outlet. Or the gas is driven by the cooling circulator to enter through the ground electrode inlet, pass through the ground electrode, the cooling circulation channel and the radio frequency electrode, and then circularly flow back into the cooling circulator through the radio frequency electrode outlet. Water cooling may be employed according to the same principle as air cooling. In the invention, the cooling channels of the radio frequency electrode and the ground electrode are communicated with each other through an external connecting pipe. One cooling channel for the ground electrode flows as in fig. 3.
Wherein, the cooling circulator can set the temperature and the flow rate of the cooling water or the cooling air.
In one embodiment of the invention, the gas chamber is provided with a uniform flow system, namely, the gas chamber comprises a uniform flow chamber and a uniform flow film, and the uniform flow chamber and the uniform flow film are arranged between the upper cover plate and the radio-frequency electrode. The uniform flow film is made of environment-friendly polyester material. The diameter of the mesh is 0.05-0.6 mm.
In one embodiment of the present invention, the flow-equalizing membrane comprises at least 1, and divides the flow-equalizing chamber into 2 or more flow-equalizing spaces, preferably 2 flow-equalizing membranes, and divides the flow-equalizing chamber into 3 flow-equalizing spaces. In a specific embodiment, as shown in fig. 2, the uniform flow chamber is divided into 3 uniform flow spaces by 2 uniform flow films.
In another embodiment of the cold plasma generator for mutation breeding according to the present invention, a gap between the parallel strips of the radio frequency electrode and the narrow groove of the ground electrode to form a plasma discharge chamber is 0.5 to 2.5mm, and an effective discharge height between the parallel strips of the radio frequency electrode and the ground electrode is 1 to 50 mm.
In one embodiment of the invention, the parallel laths of the radio frequency electrode can penetrate through the narrow grooves of the lower partition plate and parallelly penetrate between the narrow grooves of the ground electrode to form a plasma irradiation area, and the area of the irradiation area can reach 4500-6000 square millimeters.
In one embodiment of the present invention, the ground electrode structure further comprises a bottom plate disposed below the ground electrode for fixing the ground electrode structure.
In one embodiment of the invention, the air inlet mode of the air inlet channel is controlled by the air flow controller, the air inlet flow can be quantitatively controlled, and the power matching adjustment on the radio frequency power supply is favorable for generating long-time temperature stable plasma in the biological mutation breeding process, particularly in the animal and plant mutation process. The working gas entering the air inlet channel of the cold plasma generator for mutation breeding comprises inert gases such as helium, argon and the like, and can also comprise nitrogen, air and the like, and helium is preferred.
Examples
The plasma mutation breeding apparatus of the present invention will be further described below with reference to examples.
Example 1
The invention relates to a cold plasma generator for mutation breeding, which comprises an upper cover plate, a radio frequency electrode, a lower cover plate and a ground electrode, wherein the radio frequency electrode is connected with a radio frequency power supply, the ground electrode is connected with a ground wire of the radio frequency power supply, the upper cover plate covers the radio frequency electrode and forms a gas chamber with the radio frequency electrode, a gas inlet channel is arranged on the upper cover plate, the radio frequency electrode is provided with a protruded parallel lath, the ground electrode is provided with a narrow groove matched with the lath of the radio frequency electrode, and the parallel lath of the radio frequency electrode can penetrate through the narrow groove of a lower clapboard and is parallelly inserted between the narrow grooves of the ground; a plasma discharge chamber is formed between the parallel laths of the radio-frequency electrode and the narrow groove of the ground electrode, and cooling circulation channels are arranged in the radio-frequency electrode and the ground electrode, wherein the cooling channels of the radio-frequency electrode and the ground electrode form a closed-loop cooling circulation channel through connecting pipelines.
The length of the plasma generator is 79mm, the width of the plasma generator is 58mm, the gap of the plasma discharge chamber is 0.8mm, and the effective discharge height of the parallel strips of the radio-frequency electrode and the parallel strips of the ground electrode is 18 mm. Introducing helium with the purity of 99.999 percent, wherein the gas amount is 15slm, and the temperature of cooling water set by a cooling circulator is 10 ℃. After the plasma generator emitted the jet for 15 min, the flow rate of the plasma jet was detected at 36 points 2mm below the plasma emitter in the manner shown in fig. 4 using a gas flow meter, corresponding to the flow rates at the points from top to bottom and from left to right in the figure, shown in the lower gas flow rate table 1; the temperature of the plasma jet was measured at 2mm with a thermometer at 36 points, corresponding to the points from top to bottom and from left to right in the figure, shown in the lower jet temperature table 2.
TABLE 1
Figure 577247DEST_PATH_IMAGE001
The flow rates were determined at 36 sites as in table 1, with the average: 6.18 slm with a standard deviation of 0.39.
TABLE 2
Figure 399710DEST_PATH_IMAGE002
The temperature was measured at 36 sites as in table 2, with the average: 39.91 ℃ with a standard deviation of 1.98.
Example 2
In the structure of example 1, 2 uniform flow films were provided to divide the uniform flow chamber into 3 independent uniform flow spaces. Introducing helium with the purity of 99.999 percent, wherein the gas amount is 15slm, and the temperature of cooling water set by a cooling circulator is 10 ℃. After the plasma generator emitted the jet for 15 min, the flow rate and temperature of the plasma jet were respectively detected at 36 points 2mm below the plasma emitter in the manner shown in fig. 4, and the flow rate and temperature corresponding to the points from top to bottom and from left to right in the figure are shown in tables 3 and 4, respectively.
TABLE 3
Figure 276530DEST_PATH_IMAGE003
The flow rates were determined at 36 sites as in table 3, with the average: 5.77 slm with a standard deviation of 0.06.
TABLE 4
Figure 475430DEST_PATH_IMAGE004
The temperature was measured at 36 sites as in table 4, with the average: 39.12 ℃ with a standard deviation of 1.47.
The apparatus was further operated for 20min, and the temperature of the plasma jet was detected at 36 points, corresponding to the points from top to bottom and from left to right in the figure, in the manner shown in fig. 4, 2mm below the plasma emitter using a thermometer in the same manner as described above, and shown in table 5 below.
TABLE 5
Figure 409888DEST_PATH_IMAGE005
The average of the temperatures at the 36 test points was 39.36 ℃ with a standard deviation of 1.53 as shown in Table 5.
In the embodiment, the plasma jet temperature is kept stable at 39-39.5 ℃ after the plasma generator emits for 30 min and 60 min. Therefore, the jet flow temperature emitted by the plasma generator is tested, the plasma jet flow temperature is kept stable after 15 min, and the average temperature is 39-39.5 ℃.
Example 3
The invention relates to a cold plasma generator for mutation breeding, which comprises an upper cover plate, a radio frequency electrode, a lower cover plate and a ground electrode, and is characterized in that the radio frequency electrode is connected with a radio frequency power supply, the ground electrode is connected with a ground wire of the radio frequency power supply, the upper cover plate covers the radio frequency electrode and forms a gas chamber with the radio frequency electrode, a uniform flow system is arranged in the gas chamber, the gas chamber comprises a uniform flow chamber and a uniform flow film, the uniform flow chamber is divided into 3 independent uniform flow spaces by 2 uniform flow films, a gas inlet channel is arranged on the upper cover plate, the radio frequency electrode is provided with protruded parallel laths, the ground electrode is provided with narrow grooves matched with the laths of the radio frequency electrode, and the parallel laths of the radio frequency electrode can penetrate through the narrow grooves of the lower partition plate and are parallelly inserted between; a plasma discharge chamber is formed between the parallel laths of the radio-frequency electrode and the narrow groove of the ground electrode, and cooling circulation channels are arranged in the radio-frequency electrode and the ground electrode, wherein the cooling channels of the radio-frequency electrode and the ground electrode form a closed-loop cooling circulation channel through connecting pipelines.
The length of the plasma generator is 102 mm, the width of the plasma generator is 58mm, the gap of the plasma discharge chamber is 0.8mm, and the effective discharge height of the parallel strips of the radio-frequency electrode and the parallel strips of the ground electrode is 18 mm. Introducing helium with the purity of 99.999 percent, wherein the gas amount is 20 slm, and the temperature of cooling water set by a cooling circulator is 7 ℃. After the plasma generator emitted the jet for 20min, 36 points were taken 2mm below the plasma emitter to detect the flow rate and temperature of the plasma jet, which correspond to the flow rate and temperature at the points from top to bottom and from left to right in the figure, shown in tables 6 and 7, respectively.
TABLE 6
Figure 403252DEST_PATH_IMAGE006
The flow rates were determined as in table 6 at 36 points with the average: 5.73 slm with a standard deviation of 0.09.
TABLE 7
Figure 767368DEST_PATH_IMAGE007
The temperature was measured at 36 sites as in table 7, with the average: 39.42 ℃ with a standard deviation of 1.64.
According to the experimental results, the plasma generator can always emit jet flow with stable temperature in a large area in the whole operation process, the mutation treatment time can be as long as 7200 s, the requirement of microorganism mutation breeding can be met, and continuous treatment mutation breeding of a large amount of plant pollen, seeds, callus, protoplast, animal fish eggs, cells and the like can be ensured.
Industrial applicability
The cold plasma generator for mutation breeding can be manufactured and used in the field of plasma mutation breeding.
Although the embodiments of the present invention have been described above with reference to the accompanying drawings, the present invention is not limited to the above-described embodiments and application fields, and the above-described embodiments are illustrative, instructive, and not restrictive. Those skilled in the art, having the benefit of this disclosure, may effect numerous modifications thereto without departing from the scope of the invention as defined by the appended claims.

Claims (10)

1. A cold plasma generator for mutation breeding comprises an upper cover plate, a radio frequency electrode, a lower cover plate and a ground electrode, and is characterized in that the radio frequency electrode is connected with a radio frequency power supply, the ground electrode is connected with a ground wire of the radio frequency power supply, the upper cover plate covers the radio frequency electrode and forms a gas chamber with the radio frequency electrode, a gas inlet channel is arranged on the upper cover plate, the radio frequency electrode is provided with protruded parallel battens, the ground electrode is provided with narrow grooves matched with the battens of the radio frequency electrode, and the parallel battens of the radio frequency electrode can penetrate through the narrow grooves of the lower partition plate and are parallelly inserted between the narrow grooves of the ground electrode; a plasma discharge chamber is formed between the parallel laths of the radio-frequency electrode and the narrow groove of the ground electrode, and cooling circulation channels are arranged in the radio-frequency electrode and the ground electrode, wherein the cooling channels of the radio-frequency electrode and the ground electrode form a closed-loop cooling circulation channel through connecting pipelines, so that plasma with stable temperature is generated in the mutation breeding process.
2. A cold plasma generator for mutation breeding according to claim 1, wherein the average temperature of the temperature-stable plasma is 36 to 42 ℃ and the standard deviation is within 2 ℃.
3. The cold plasma generator for mutation breeding according to claim 1 or 2, wherein the cooling circulation inside the radio frequency electrode and the ground electrode adopts a water cooling circulation or an air cooling circulation, and the water cooling circulation or the air cooling circulation drives cooling water or gas to enter through the inlet by the cooling circulation machine, pass through the radio frequency electrode, the cooling circulation channel and the ground electrode, and then return to the cooling circulation machine through the outlet.
4. A cold plasma generator for mutation breeding according to any one of claims 1 to 3, wherein a uniform flow system is provided in the gas chamber, the uniform flow system comprises a uniform flow chamber and a uniform flow film, and the uniform flow chamber and the uniform flow film are provided between the upper cover plate and the radio frequency electrode.
5. A cold plasma generator for mutation breeding according to any one of claims 1 to 4, wherein said flow equalizing membrane comprises at least 1, and the flow equalizing chamber is partitioned into 2 or more flow equalizing spaces, preferably 2 flow equalizing membranes, and the flow equalizing chamber is partitioned into 3 flow equalizing spaces.
6. A cold plasma generator for mutation breeding according to claim 4, wherein the flow-equalizing membrane is made of a polymer material, such as an eco-polyester material, and the diameter of the mesh of the flow-equalizing membrane is 0.05 to 0.6 mm.
7. A cold plasma generator for mutation breeding according to any one of claims 1 to 6, wherein the gap between the parallel strips of the radio-frequency electrode and the narrow groove of the ground electrode to form a plasma discharge chamber is 0.5 to 2.5mm, and the effective discharge height of the parallel strips of the radio-frequency electrode and the ground electrode is 1 to 50 mm.
8. A cold plasma generator for mutation breeding according to any one of claims 1 to 7, wherein the parallel strips of the radio frequency electrode can penetrate through the narrow grooves of the lower partition plate and parallelly penetrate between the narrow grooves of the ground electrode to form a plasma irradiation area, and the area of the irradiation area can reach 4500-6000 square millimeters.
9. A cold plasma generator for mutation breeding according to any one of claims 1 to 8, wherein a helium gas with a purity of 99.999% is used as a working gas source, a gas flow rate is 15 to 20 SLM, and a RF power supply power is 300 to 500W, a cooling water temperature set by a cooling circulator is 6 to 10 ℃, and a plasma average temperature is 37 to 40 ℃oAnd C, generating plasma, and keeping the temperature stable after working for 20 min.
10. The cold plasma generator for mutation breeding according to any one of claims 1 to 9, further comprising a bottom plate disposed below the ground electrode.
CN201811009063.8A 2018-08-31 2018-08-31 Cold plasma generator for mutation breeding Pending CN110876222A (en)

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CN114286488A (en) * 2021-12-30 2022-04-05 南京工业大学 Atmospheric pressure large-scale DBD material modification device based on gas circuit modularization
CN114286488B (en) * 2021-12-30 2023-02-28 南京工业大学 Atmospheric pressure large-scale DBD material modification device based on gas circuit modularization

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