CN110865066A - Raman spectrum sample box with total reflection structure - Google Patents
Raman spectrum sample box with total reflection structure Download PDFInfo
- Publication number
- CN110865066A CN110865066A CN201911127415.4A CN201911127415A CN110865066A CN 110865066 A CN110865066 A CN 110865066A CN 201911127415 A CN201911127415 A CN 201911127415A CN 110865066 A CN110865066 A CN 110865066A
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- sample
- raman spectrum
- reflecting film
- raman
- sample box
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- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N21/00—Investigating or analysing materials by the use of optical means, i.e. using sub-millimetre waves, infrared, visible or ultraviolet light
- G01N21/62—Systems in which the material investigated is excited whereby it emits light or causes a change in wavelength of the incident light
- G01N21/63—Systems in which the material investigated is excited whereby it emits light or causes a change in wavelength of the incident light optically excited
- G01N21/65—Raman scattering
- G01N21/658—Raman scattering enhancement Raman, e.g. surface plasmons
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- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N21/00—Investigating or analysing materials by the use of optical means, i.e. using sub-millimetre waves, infrared, visible or ultraviolet light
- G01N21/62—Systems in which the material investigated is excited whereby it emits light or causes a change in wavelength of the incident light
- G01N21/63—Systems in which the material investigated is excited whereby it emits light or causes a change in wavelength of the incident light optically excited
- G01N21/65—Raman scattering
-
- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N21/00—Investigating or analysing materials by the use of optical means, i.e. using sub-millimetre waves, infrared, visible or ultraviolet light
- G01N21/62—Systems in which the material investigated is excited whereby it emits light or causes a change in wavelength of the incident light
- G01N21/63—Systems in which the material investigated is excited whereby it emits light or causes a change in wavelength of the incident light optically excited
- G01N21/65—Raman scattering
- G01N2021/651—Cuvettes therefore
Abstract
The invention discloses a Raman spectrum sample box with a total reflection structure, which consists of a sample input and excitation light incident port, a gold reflecting film and a Raman spectrum signal output port; injecting a cell sample into the sample box from the sample input and exciting light incident port, wherein the cell can grow on the gold reflecting film; during detection, a non-focused low-energy laser beam irradiates a cell sample from a sample input and excitation light incident port, the transmitted and refracted light can irradiate the sample again to excite Raman spectrum for multiple times after being reflected on the gold reflecting film, and after the excited Raman scattered light passes through the multiple reflection process of the gold reflecting film, a part of Raman light escapes from the sample box through a Raman spectrum signal output port to be collected by a detector. The invention can nondestructively detect the integral Raman spectrum information of the cell sample, and can analyze the change of the fine Raman spectrum information in the cell division and propagation process through in-situ tracking detection, thereby providing help for solving the cell metabolism process.
Description
Technical Field
The invention belongs to the technical field of Raman spectroscopy, is particularly suitable for Raman spectroscopy nondestructive testing of cell samples, and particularly relates to a Raman spectroscopy sample box with a total reflection structure.
Background
The optical path of the existing Raman spectrometer basically adopts a scheme that an excitation optical path and a collection optical path share one objective lens, focusing light is irradiated on a sample and sample Raman spectrum information on a focusing point is collected, and the Raman spectrometer has good collection efficiency of Raman spectrum, but has the defects. Firstly, for raman scattering spectroscopy, the objective lens can only collect a small part of the spectral signals. In order to enhance the Raman spectrum signal, a surface enhanced Raman spectrum technology can be combined; secondly, only the spectrum information of the focus point part of the sample can be detected, and the spectrum information of the whole sample cannot be obtained at one time; thirdly, the energy of the focus point is high, so that the biological cell sample is fatally damaged, and long-time tracking detection cannot be carried out.
Disclosure of Invention
The purpose of the invention is as follows: the invention provides a Raman spectrum sample box with a total reflection structure, and aims to solve the problems that the existing Raman spectrometer cannot detect the integral Raman spectrum information of cells and cannot detect the cells for a long time in a nondestructive mode.
The technical scheme is as follows: a Raman spectrum sample box with a total reflection structure is composed of a sample input and excitation light incident port, a gold reflecting film and a Raman spectrum signal output port;
a cell sample is put into the Raman spectrum sample box from a sample input port and an exciting light incident port, a gold reflecting film is plated on the inner wall of the Raman spectrum sample box, the compatibility of the cell and the gold reflecting film is good, and the cell can grow on the gold reflecting film;
during detection, a non-focused low-energy laser beam irradiates a cell sample from a sample input and excitation light entrance port, the transmitted and refracted light can irradiate the sample again to excite Raman spectrum for multiple times after being reflected on the gold reflecting film, and after the excited Raman scattered light passes through the multiple reflection process of the gold reflecting film, a part of Raman light escapes from the sample box through a Raman spectrum signal output port and is collected by a detector.
As an optimization: the Raman spectrum sample box is made of glass or other metal and nonmetal materials.
As an optimization: the preparation of the gold reflecting film adopts a physical vacuum evaporation method or a magnetron sputtering method.
Has the advantages that: the sample kit of the invention is particularly suitable for raman detection of biological cell samples. The invention allows non-focused excitation light with lower energy to irradiate the interior of the sample box from the upper incident hole, excites the Raman spectrum signal of the whole cell sample through the process of continuous reflection in the interior on the premise of not damaging the cell, outputs the Raman spectrum signal from the side surface of the sample box through the process of multiple reflections, and collects the Raman signal by the detector at the side surface.
The invention can nondestructively detect the integral Raman spectrum information of the cell sample, and can analyze the change of the fine Raman spectrum information in the cell division and propagation process through in-situ tracking detection, thereby providing help for solving the cell metabolism process.
Drawings
FIG. 1 is a schematic view of the overall structure of the present invention;
FIG. 2 is a schematic top view of the present invention;
fig. 3 is a side view of the present invention.
Detailed Description
The technical solutions in the embodiments of the present invention will be clearly and completely described below so that those skilled in the art can better understand the advantages and features of the present invention, and thus the scope of the present invention will be more clearly defined. The embodiments described herein are only a few embodiments of the present invention, rather than all embodiments, and all other embodiments that can be derived by one of ordinary skill in the art without inventive faculty based on the embodiments described herein are intended to fall within the scope of the present invention.
Examples
As shown in FIGS. 1-3, a Raman spectrum sample box with a total reflection structure, the Raman spectrum sample box 4 is composed of a sample input and excitation light incident port 1, a gold reflecting film 2 and a Raman spectrum signal output port 3. The Raman spectrum sample box 4 is made of glass or other metal and nonmetal materials.
A cell sample is injected into the Raman spectrum sample box 4 from the sample feeding port and the exciting light incidence port 1, the inner wall of the Raman spectrum sample box 4 is plated with the gold reflecting film 2, the compatibility of the cell and the gold reflecting film 2 is good, and the cell can grow on the gold reflecting film 2.
During detection, a non-focused low-energy laser beam irradiates a cell sample from the sample input and excitation light incident port 1, the transmitted and refracted light of the non-focused low-energy laser beam is reflected on the gold reflecting film 2 and then irradiates the sample again to excite Raman spectra for multiple times, and after the excited Raman scattered light passes through the multiple reflection process of the gold reflecting film 2, a part of the Raman light escapes from the sample box through the Raman spectrum signal output port 3 and is collected by a detector.
The preparation of the gold reflecting film 2 adopts a physical vacuum evaporation method or a magnetron sputtering method. The gold reflective film 2 is characterized by high reflectivity, environmental stability and biocompatibility. The higher reflectivity can ensure that the exciting light is reflected for multiple times in the sample box so as to realize multiple excitation of the same incident light to the sample, and can also ensure that the Raman scattering light is reflected for multiple times in the sample box and is guided to the Raman spectrum signal output port 3. The gold reflective film 2 has environmental stability, and can prevent the gold film from being corroded and polluted by substances such as solvents and the like, and maintain high reflective characteristics and high quality of the film all the time. The biocompatibility of the gold reflecting film 2 can ensure the stable growth of biological cells in the sample box, thereby being beneficial to long-time tracking observation. The above characteristics of the gold reflective film 2 in the sample box ensure that the biological sample excited by the non-focused low-energy laser beam can detect the Raman spectrum and maintain the biological activity of the biological sample.
The sample box is particularly suitable for Raman detection of biological cell samples, the non-focusing excitation light with lower energy is allowed to irradiate the interior of the sample box from the upper incident hole, the Raman spectrum signals of the whole cell sample are excited through the internal continuous reflection process on the premise of not damaging cells, the Raman spectrum signals are output from the side face of the sample box through the multiple reflection process, and the Raman signals are collected by the side face detector.
The invention can nondestructively detect the integral Raman spectrum information of the cell sample, and can analyze the change of the fine Raman spectrum information in the cell division and propagation process through in-situ tracking detection, thereby providing help for solving the cell metabolism process.
Claims (3)
1. A Raman spectrum sample box with a total reflection structure is characterized in that: the Raman spectrum sample box (4) consists of a sample input and excitation light incident port (1), a gold reflecting film (2) and a Raman spectrum signal output port (3);
a cell sample is put into the Raman spectrum sample box (4) from the sample input port and the exciting light incident port (1), the inner wall of the Raman spectrum sample box (4) is plated with a gold reflecting film (2), and the cell and the gold reflecting film (2) have good compatibility and can grow on the gold reflecting film (2);
during detection, a non-focused low-energy laser beam irradiates a cell sample from a sample input and excitation light incident port (1), transmitted and refracted light of the non-focused low-energy laser beam is reflected on a gold reflecting film (2) and then can irradiate the sample again to carry out multiple times of Raman spectrum excitation, and after the excited Raman scattered light passes through the multiple times of reflection process of the gold reflecting film (2), a part of Raman light escapes from a sample box through a Raman spectrum signal output port (3) and is collected by a detector.
2. A raman spectroscopy sample box of total reflection structure according to claim 1, characterized in that: the Raman spectrum sample box (4) is made of glass or other metal and nonmetal materials.
3. A raman spectroscopy sample box of total reflection structure according to claim 1, characterized in that: the preparation of the gold reflecting film (2) adopts a physical vacuum evaporation method or a magnetron sputtering method.
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| Application Number | Priority Date | Filing Date | Title |
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| CN201911127415.4A CN110865066A (en) | 2019-11-18 | 2019-11-18 | Raman spectrum sample box with total reflection structure |
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| Application Number | Priority Date | Filing Date | Title |
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| CN201911127415.4A CN110865066A (en) | 2019-11-18 | 2019-11-18 | Raman spectrum sample box with total reflection structure |
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| CN110865066A true CN110865066A (en) | 2020-03-06 |
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| CN201911127415.4A Pending CN110865066A (en) | 2019-11-18 | 2019-11-18 | Raman spectrum sample box with total reflection structure |
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Citations (6)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN103630493A (en) * | 2013-07-23 | 2014-03-12 | 中国科学院电子学研究所 | Raman spectrum measurement system |
| CN105651702A (en) * | 2016-03-22 | 2016-06-08 | 派棱环保科技（苏州）有限公司 | Water quality detection method based on film-coated decomposition pool |
| WO2017125374A1 (en) * | 2016-01-21 | 2017-07-27 | Leibniz - Institut Für Analytische Wissenschaften -Isas- E.V. | Device for detecting and characterising organic molecules in a liquid sample volume |
| CN107532992A (en) * | 2015-04-24 | 2018-01-02 | 株式会社岛津制作所 | Optical detecting device |
| CN209446469U (en) * | 2018-11-27 | 2019-09-27 | 北京工业大学 | Body towards liquid sensing detection enhances Raman spectroscopy test device |
| US20200264050A1 (en) * | 2019-02-15 | 2020-08-20 | Wayne State University | Apparatuses, systems, and methods for detecting materials based on raman spectroscopy |
-
2019
- 2019-11-18 CN CN201911127415.4A patent/CN110865066A/en active Pending
Patent Citations (6)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN103630493A (en) * | 2013-07-23 | 2014-03-12 | 中国科学院电子学研究所 | Raman spectrum measurement system |
| CN107532992A (en) * | 2015-04-24 | 2018-01-02 | 株式会社岛津制作所 | Optical detecting device |
| WO2017125374A1 (en) * | 2016-01-21 | 2017-07-27 | Leibniz - Institut Für Analytische Wissenschaften -Isas- E.V. | Device for detecting and characterising organic molecules in a liquid sample volume |
| CN105651702A (en) * | 2016-03-22 | 2016-06-08 | 派棱环保科技（苏州）有限公司 | Water quality detection method based on film-coated decomposition pool |
| CN209446469U (en) * | 2018-11-27 | 2019-09-27 | 北京工业大学 | Body towards liquid sensing detection enhances Raman spectroscopy test device |
| US20200264050A1 (en) * | 2019-02-15 | 2020-08-20 | Wayne State University | Apparatuses, systems, and methods for detecting materials based on raman spectroscopy |
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Application publication date: 20200306 |