CN110863018A - Method for preparing fuel ethanol by using corn - Google Patents
Method for preparing fuel ethanol by using corn Download PDFInfo
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- CN110863018A CN110863018A CN201911199722.3A CN201911199722A CN110863018A CN 110863018 A CN110863018 A CN 110863018A CN 201911199722 A CN201911199722 A CN 201911199722A CN 110863018 A CN110863018 A CN 110863018A
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- Prior art keywords
- corn
- fuel ethanol
- corn kernels
- washing
- preparing fuel
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- 240000008042 Zea mays Species 0.000 title claims abstract description 70
- 235000005824 Zea mays ssp. parviglumis Nutrition 0.000 title claims abstract description 70
- 235000002017 Zea mays subsp mays Nutrition 0.000 title claims abstract description 70
- 235000005822 corn Nutrition 0.000 title claims abstract description 70
- LFQSCWFLJHTTHZ-UHFFFAOYSA-N Ethanol Chemical compound CCO LFQSCWFLJHTTHZ-UHFFFAOYSA-N 0.000 title claims abstract description 52
- 239000000446 fuel Substances 0.000 title claims abstract description 27
- 238000000034 method Methods 0.000 title claims abstract description 24
- 238000005406 washing Methods 0.000 claims abstract description 23
- HEMHJVSKTPXQMS-UHFFFAOYSA-M Sodium hydroxide Chemical compound [OH-].[Na+] HEMHJVSKTPXQMS-UHFFFAOYSA-M 0.000 claims abstract description 21
- VEXZGXHMUGYJMC-UHFFFAOYSA-N Hydrochloric acid Chemical compound Cl VEXZGXHMUGYJMC-UHFFFAOYSA-N 0.000 claims abstract description 16
- 238000001035 drying Methods 0.000 claims abstract description 16
- 239000000203 mixture Substances 0.000 claims abstract description 15
- 239000000843 powder Substances 0.000 claims abstract description 14
- 239000006228 supernatant Substances 0.000 claims abstract description 14
- 239000004382 Amylase Substances 0.000 claims abstract description 13
- 102000013142 Amylases Human genes 0.000 claims abstract description 13
- 108010065511 Amylases Proteins 0.000 claims abstract description 13
- 240000004808 Saccharomyces cerevisiae Species 0.000 claims abstract description 13
- 235000019418 amylase Nutrition 0.000 claims abstract description 13
- 238000010438 heat treatment Methods 0.000 claims abstract description 13
- XLYOFNOQVPJJNP-UHFFFAOYSA-N water Substances O XLYOFNOQVPJJNP-UHFFFAOYSA-N 0.000 claims abstract description 13
- 230000000694 effects Effects 0.000 claims abstract description 9
- 239000012535 impurity Substances 0.000 claims abstract description 9
- 238000000227 grinding Methods 0.000 claims abstract description 7
- 238000006386 neutralization reaction Methods 0.000 claims abstract description 7
- 238000003756 stirring Methods 0.000 claims abstract description 7
- 238000005303 weighing Methods 0.000 claims abstract description 7
- 238000004140 cleaning Methods 0.000 claims abstract description 4
- 238000007789 sealing Methods 0.000 claims abstract description 4
- 238000011081 inoculation Methods 0.000 claims description 6
- 238000000855 fermentation Methods 0.000 claims description 5
- 230000004151 fermentation Effects 0.000 claims description 5
- 102000004190 Enzymes Human genes 0.000 claims description 3
- 108090000790 Enzymes Proteins 0.000 claims description 3
- 238000005119 centrifugation Methods 0.000 claims description 3
- 239000002994 raw material Substances 0.000 abstract description 4
- 238000005299 abrasion Methods 0.000 abstract description 3
- 238000009776 industrial production Methods 0.000 abstract description 2
- 239000000047 product Substances 0.000 description 4
- CURLTUGMZLYLDI-UHFFFAOYSA-N Carbon dioxide Chemical compound O=C=O CURLTUGMZLYLDI-UHFFFAOYSA-N 0.000 description 2
- 229920002261 Corn starch Polymers 0.000 description 2
- 239000008120 corn starch Substances 0.000 description 2
- 235000013305 food Nutrition 0.000 description 2
- 210000003491 skin Anatomy 0.000 description 2
- 239000002028 Biomass Substances 0.000 description 1
- 108010068370 Glutens Proteins 0.000 description 1
- 241001464837 Viridiplantae Species 0.000 description 1
- 239000004566 building material Substances 0.000 description 1
- 239000001569 carbon dioxide Substances 0.000 description 1
- 229910002092 carbon dioxide Inorganic materials 0.000 description 1
- 230000001054 cortical effect Effects 0.000 description 1
- 239000003814 drug Substances 0.000 description 1
- 238000009837 dry grinding Methods 0.000 description 1
- 238000005265 energy consumption Methods 0.000 description 1
- 210000002615 epidermis Anatomy 0.000 description 1
- 239000000835 fiber Substances 0.000 description 1
- 230000008676 import Effects 0.000 description 1
- 239000003112 inhibitor Substances 0.000 description 1
- 239000007788 liquid Substances 0.000 description 1
- 238000004519 manufacturing process Methods 0.000 description 1
- -1 papermaking Substances 0.000 description 1
- 239000003208 petroleum Substances 0.000 description 1
- 230000029553 photosynthesis Effects 0.000 description 1
- 238000010672 photosynthesis Methods 0.000 description 1
- 238000000053 physical method Methods 0.000 description 1
- 238000003672 processing method Methods 0.000 description 1
- 239000007787 solid Substances 0.000 description 1
- 239000000126 substance Substances 0.000 description 1
- 239000004753 textile Substances 0.000 description 1
- 239000002699 waste material Substances 0.000 description 1
Classifications
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12P—FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE
- C12P7/00—Preparation of oxygen-containing organic compounds
- C12P7/02—Preparation of oxygen-containing organic compounds containing a hydroxy group
- C12P7/04—Preparation of oxygen-containing organic compounds containing a hydroxy group acyclic
- C12P7/06—Ethanol, i.e. non-beverage
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12P—FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE
- C12P19/00—Preparation of compounds containing saccharide radicals
- C12P19/14—Preparation of compounds containing saccharide radicals produced by the action of a carbohydrase (EC 3.2.x), e.g. by alpha-amylase, e.g. by cellulase, hemicellulase
-
- Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
- Y02—TECHNOLOGIES OR APPLICATIONS FOR MITIGATION OR ADAPTATION AGAINST CLIMATE CHANGE
- Y02E—REDUCTION OF GREENHOUSE GAS [GHG] EMISSIONS, RELATED TO ENERGY GENERATION, TRANSMISSION OR DISTRIBUTION
- Y02E50/00—Technologies for the production of fuel of non-fossil origin
- Y02E50/10—Biofuels, e.g. bio-diesel
Landscapes
- Organic Chemistry (AREA)
- Chemical & Material Sciences (AREA)
- Engineering & Computer Science (AREA)
- Zoology (AREA)
- Life Sciences & Earth Sciences (AREA)
- Wood Science & Technology (AREA)
- Chemical Kinetics & Catalysis (AREA)
- Microbiology (AREA)
- General Chemical & Material Sciences (AREA)
- Biotechnology (AREA)
- Health & Medical Sciences (AREA)
- Biochemistry (AREA)
- Bioinformatics & Cheminformatics (AREA)
- General Engineering & Computer Science (AREA)
- General Health & Medical Sciences (AREA)
- Genetics & Genomics (AREA)
- Preparation Of Compounds By Using Micro-Organisms (AREA)
Abstract
The invention discloses a method for preparing fuel ethanol by using corn, which comprises the following steps: step 1, weighing corn kernels, putting the corn kernels into a washing tank, adding clear water for cleaning, and then adding dilute hydrochloric acid for washing; step 2, adding sodium hydroxide into the washed corn kernels for neutralization to neutrality, drying the neutralized corn kernels, and then grinding to obtain corn powder; step 3, adding amylase into the corn powder, heating and stirring for saccharification to obtain a mixture; and 4, centrifuging the mixture, taking supernatant, adding yeast into the supernatant, and sealing and fermenting to obtain the fuel ethanol. The method can more effectively remove ash and skin impurities through the pretreatment of dilute hydrochloric acid, reduce the abrasion of equipment, improve the yield, and has the advantages of high purity of the prepared fuel ethanol, stable system, good amylase saccharification effect, low raw material cost, simple process, simple and convenient operation and easy industrial production.
Description
Technical Field
The invention belongs to the technical field of biological energy, and relates to a method for preparing fuel ethanol by using corn.
Background
Biological energy is used as environment-friendly energy and takes organisms as energy of a carrier. Directly or indirectly from photosynthesis of green plants, can be converted into conventional solid, liquid and gaseous fuels, is inexhaustible, and has special advantages in the development of biological energy sources: the forestry biomass energy raw materials are rich, non-grain energy crops can be planted on the marginal land, wastes of agriculture and forestry can be used as the biological energy raw materials, the existing energy consumption structure can be improved by developing biological energy, the dependence degree of petroleum import is reduced, carbon dioxide emission is reduced, the ecological environment is improved, and the marginal land can be fully utilized.
Disclosure of Invention
The invention aims to provide a method for preparing fuel ethanol by using corn, which solves the problems of easy generation of fermentation inhibitors and high production cost in the prior art.
The technical scheme adopted by the invention is that the method for preparing the fuel ethanol by using the corn specifically comprises the following steps:
step 1, weighing corn kernels, putting the corn kernels into a washing tank, adding clear water for cleaning, and then adding dilute hydrochloric acid for washing to remove ash and epidermal impurities;
step 2, adding sodium hydroxide into the washed corn kernels for neutralization to neutrality, drying the neutralized corn kernels, and then grinding to obtain corn powder;
step 3, adding water into the corn powder, adjusting the pH value, then adding amylase, heating and stirring for saccharification to obtain a mixture;
and 4, centrifuging the mixture, taking supernatant, adding yeast into the supernatant, and sealing and fermenting to obtain the fuel ethanol.
In the step 1, the concentration of the dilute hydrochloric acid is 0.01-0.05%, the washing time is 10-12 h, and the washing temperature is 50-60 ℃.
In the step 2, the drying temperature is 40-50 ℃, and the drying time is 1-2 h.
In the step 3, the pH value is adjusted to be 5.0-6.5, the enzyme activity is 50000-60000 u/mL, and the heating temperature is 80-100 ℃.
In the step 4, the centrifugation time is 10-20 min.
In step 4, the yeast inoculation amount is 1.5X 107～1.8×107One per mL, and the fermentation time is 2-5 days.
The method has the advantages that the dilute hydrochloric acid pretreatment can more effectively remove ash and skin impurities, reduce the abrasion of equipment and improve the yield, and the prepared fuel ethanol has high purity, stable system and good amylase saccharification effect.
Detailed Description
The present invention will be described in detail with reference to the following embodiments.
The method for preparing fuel ethanol by using corn comprises the following steps:
step 1, weighing corn kernels, putting the corn kernels into a washing tank, adding clear water for cleaning, and then adding dilute hydrochloric acid for washing to remove ash and epidermal impurities;
step 2, adding sodium hydroxide into the washed corn kernels for neutralization to neutrality, drying the neutralized corn kernels, and then grinding to obtain corn powder;
step 3, adding water into the corn powder, adjusting the pH value, then adding amylase, heating and stirring for saccharification to obtain a mixture;
and 4, centrifuging the mixture, taking supernatant, adding yeast into the supernatant, and sealing and fermenting to obtain the fuel ethanol.
In the step 1, the concentration of the dilute hydrochloric acid is 0.01-0.05%, the washing time is 10-12 h, and the washing temperature is 50-60 ℃.
In the step 2, the drying temperature is 40-50 ℃, and the drying time is 1-2 h.
In the step 3, the pH value is adjusted to be 5.0-6.5, the enzyme activity is 50000-60000 u/mL, and the heating temperature is 80-100 ℃.
In the step 4, the centrifugation time is 10-20 min.
In step 4, the yeast inoculation amount is 1.5X 107～1.8×107One per mL, and the fermentation time is 2-5 days.
The corn kernel consists of four parts of epidermis, endosperm, germ and root cap, and the processing method is divided into a dry method and a wet method. The dry method refers to dry milling of corn, and the product is mainly used in various food, feed and fermentation industries. The wet processing is to divide corn kernels into five products of corn steep liquor, corn starch, corn germ, corn gluten protein, cortical fiber and the like by adopting a physical method, wherein the corn starch is a main product and can be directly eaten or reprocessed, and all the products are widely applied to the industries of food, textile, papermaking, chemical industry, medicine, building materials and the like.
Example 1
The method for preparing fuel ethanol by using corn comprises the following steps:
step 1, weighing corn kernels, putting the corn kernels into a washing tank, adding clear water to clean the corn kernels, then adding dilute hydrochloric acid to wash the corn kernels, wherein the concentration of the dilute hydrochloric acid is 0.05%, the washing time is 10 hours, the washing temperature is 60 ℃, and ash and epidermal impurities are removed;
step 2, adding sodium hydroxide into the washed corn kernels for neutralization to neutrality, drying the neutralized corn kernels at the drying temperature of 40 ℃ for 2 hours, and then grinding to obtain corn powder;
step 3, adding water into the corn powder, adjusting the pH value to 5.0, then adding amylase, wherein the activity of the amylase is 60000u/mL, heating and stirring for saccharification, and heating the mixture to 80 ℃ to obtain a mixture;
step 4, centrifuging the mixture for 20min, taking supernatant, adding yeast into the supernatant, wherein the yeast inoculation amount is 1.5 multiplied by 107And (5) fermenting for 5 days in a sealed manner to obtain the fuel ethanol.
Example 2
The method for preparing fuel ethanol by using corn comprises the following steps:
step 1, weighing corn kernels, putting the corn kernels into a washing tank, adding clear water to clean the corn kernels, then adding dilute hydrochloric acid to wash the corn kernels, wherein the concentration of the dilute hydrochloric acid is 0.01%, the washing time is 12 hours, the washing temperature is 50 ℃, and ash and epidermal impurities are removed;
step 2, adding sodium hydroxide into the washed corn kernels for neutralization to neutrality, drying the neutralized corn kernels at the drying temperature of 50 ℃ for 1 hour, and then grinding to obtain corn powder;
step 3, adding water into the corn powder, adjusting the pH value to 6.5, then adding amylase, wherein the activity of the amylase is 50000u/mL, heating and stirring for saccharification, and heating the temperature to 100 ℃ to obtain a mixture;
step 4, centrifuging the mixture for 10min, taking supernatant, adding yeast into the supernatant, wherein the yeast inoculation amount is 1.8 multiplied by 107And (4) fermenting for 2 days in a sealed manner to obtain the fuel ethanol.
Example 3
The method for preparing fuel ethanol by using corn comprises the following steps:
step 1, weighing corn kernels, putting the corn kernels into a washing tank, adding clear water to clean the corn kernels, then adding dilute hydrochloric acid to wash the corn kernels, wherein the concentration of the dilute hydrochloric acid is 0.03%, the washing time is 11 hours, the washing temperature is 55 ℃, and ash and epidermal impurities are removed;
step 2, adding sodium hydroxide into the washed corn kernels for neutralization to neutrality, drying the neutralized corn kernels at the drying temperature of 45 ℃ for 1.5h, and then grinding to obtain corn powder;
step 3, adding water into the corn powder, adjusting the pH value to 6.0, then adding amylase, wherein the activity of the amylase is 55000u/mL, heating and stirring for saccharification, and the heating temperature is 90 ℃ to obtain a mixture;
step 4, centrifuging the mixture for 15min, taking supernatant, adding yeast into the supernatant, wherein the yeast inoculation amount is 1.6 multiplied by 107And (4) fermenting for 3 days in a sealed manner to obtain the fuel ethanol.
The method can more effectively remove ash and skin impurities through the pretreatment of dilute hydrochloric acid, reduce the abrasion of equipment, improve the yield, and has the advantages of high purity of the prepared fuel ethanol, stable system, good amylase saccharification effect, low raw material cost, simple process, simple and convenient operation and easy industrial production.
Claims (6)
1. The method for preparing fuel ethanol by using corn is characterized by comprising the following steps:
step 1, weighing corn kernels, putting the corn kernels into a washing tank, adding clear water for cleaning, and then adding dilute hydrochloric acid for washing to remove ash and epidermal impurities;
step 2, adding sodium hydroxide into the washed corn kernels for neutralization to neutrality, drying the neutralized corn kernels, and then grinding to obtain corn powder;
step 3, adding water into the corn powder, adjusting the pH value, then adding amylase, heating and stirring for saccharification to obtain a mixture;
and 4, centrifuging the mixture, taking supernatant, adding yeast into the supernatant, and sealing and fermenting to obtain the fuel ethanol.
2. The method for preparing fuel ethanol from corn as claimed in claim 1, wherein in the step 1, the concentration of the dilute hydrochloric acid is 0.01-0.05%, the washing time is 10-12 h, and the washing temperature is 50-60 ℃.
3. The method for preparing fuel ethanol from corn according to claim 1, wherein in the step 2, the drying temperature is 40-50 ℃ and the drying time is 1-2 h.
4. The method for preparing fuel ethanol from corn as claimed in claim 1, wherein in the step 3, the pH value is adjusted to 5.0-6.5, the enzyme activity is 50000-60000 u/mL, and the heating temperature is 80-100 ℃.
5. The method for preparing fuel ethanol from corn as claimed in claim 1, wherein in the step 4, the centrifugation time is 10-20 min.
6. The method for preparing fuel ethanol from corn as claimed in claim 1, wherein the yeast inoculation amount in step 4 is 1.5 x 107～1.8×107One per mL, and the fermentation time is 2-5 days.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201911199722.3A CN110863018A (en) | 2019-11-29 | 2019-11-29 | Method for preparing fuel ethanol by using corn |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201911199722.3A CN110863018A (en) | 2019-11-29 | 2019-11-29 | Method for preparing fuel ethanol by using corn |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| CN110863018A true CN110863018A (en) | 2020-03-06 |
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| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN201911199722.3A Pending CN110863018A (en) | 2019-11-29 | 2019-11-29 | Method for preparing fuel ethanol by using corn |
Country Status (1)
| Country | Link |
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| CN (1) | CN110863018A (en) |
Citations (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| US20140273140A1 (en) * | 2013-03-12 | 2014-09-18 | Langhauser Associates, Inc. | Simultaneous Food And Fuel Corn Refining |
| CN105463032A (en) * | 2015-12-14 | 2016-04-06 | 山西大同大学 | Pretreatment method for raw material cassava of fuel ethanol |
-
2019
- 2019-11-29 CN CN201911199722.3A patent/CN110863018A/en active Pending
Patent Citations (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| US20140273140A1 (en) * | 2013-03-12 | 2014-09-18 | Langhauser Associates, Inc. | Simultaneous Food And Fuel Corn Refining |
| CN105463032A (en) * | 2015-12-14 | 2016-04-06 | 山西大同大学 | Pretreatment method for raw material cassava of fuel ethanol |
Non-Patent Citations (1)
| Title |
|---|
| 高宗颖 等: ""用玉米生料发酵生产酒精"", 《农产品加工》 * |
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Application publication date: 20200306 |
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