CN110862883A

CN110862883A - Anti-mite and anti-oxidation medicated soap for expelling and killing mites and preparation method thereof

Info

Publication number: CN110862883A
Application number: CN201911189632.6A
Authority: CN
Inventors: 刘伟毅; 王珍明; 徐尧
Original assignee: SHANGHAI SOAPMAKING CO Ltd
Current assignee: SHANGHAI SOAPMAKING CO Ltd
Priority date: 2019-11-28
Filing date: 2019-11-28
Publication date: 2020-03-06

Abstract

The invention relates to an anti-acarid and acarid-killing anti-oxidation medicinal soap and a preparation method thereof, wherein a soap base of the medicinal soap contains 0.3-2% of herbal extraction and purification components, the herbal extraction and purification components are extracted and purified by a skin-refreshing formula consisting of four herbal raw materials, and the skin-refreshing formula is prepared from lotus leaves, ligusticum sinense, agastache rugosus and clove according to the ratio of (5-15): (10-25): (10-25): (15-35) in terms of weight ratio. Compared with the prior art, the medicinal soap has good effects of resisting bacteria, inhibiting bacteria, resisting inflammation and viruses, expelling and killing mites, resisting oxidation and the like.

Description

Anti-mite and anti-oxidation medicated soap for expelling and killing mites and preparation method thereof

Technical Field

The invention relates to a medicated soap, in particular to an anti-mite and anti-oxidation medicated soap for expelling and killing mites and a preparation method thereof.

Background

The medicated soap is prepared by adding a certain amount of bactericide into beef tallow, coconut oil or other grease, a small amount of washing aid, caustic soda and the like. The preparation process of the medicated soap is the same as that of a washing soap.

The current medicated soap has the functions of cleaning and decontaminating, and generally has the function of sterilization.

The Chinese herbal medicine soap is prepared by adding the extract of Chinese herbal medicine into soap base and adding perfume. The Chinese herbal medicine soap has small irritation to skin, mild performance, auxiliary curative effect on partial skin diseases and no obvious side effect.

At present, common medicated soaps mainly have antibacterial and bacteriostatic functions, but the requirements of people on medicated soap products are not limited to cleaning and bacteriostasis any more, but people hope to have more skin-care efficacy performance while cleaning skin. At present, more solid soap type face cleaning products appear on the market, and some products have better cleaning capability, but have stronger alkalinity and high irritation to skin due to low-carbon fatty acid, so that the skin can feel tight after washing; some products have good cleaning effect and are easy to cause damage to the skin.

Disclosure of Invention

The invention aims to overcome the defects of the prior art and provide an anti-mite and anti-oxidation medical soap for repelling mites and killing mites and a preparation method thereof.

The purpose of the invention can be realized by the following technical scheme:

the anti-oxidation medical soap for expelling and killing mites is characterized in that a soap base of the medical soap contains herbal extraction and purification components with the weight ratio of 0.5-3.0%, the herbal extraction and purification components are extracts and purified by a skin reviving formula consisting of four herbal raw materials, and the skin reviving formula is prepared from lotus leaves, ligusticum sinense, agastache rugosus and clove according to the ratio of (5-15): (10-25): (10-25): (15-35) in terms of weight ratio.

Further, the soap base is a commercially available soap base, the content of fatty acid in the soap base is more than or equal to 78 percent, the content of free alkali (calculated by sodium hydroxide) is less than or equal to 0.2 percent, the content of chloride (calculated by NaCL) is less than or equal to 0.7 percent, the content of ethanol insoluble substances is less than or equal to 2 percent, and the content of water and volatile substances is less than or equal to 14 percent; the fatty acid solidification point is 39-43 ℃.

The soap base (sodium fatty acid) is sodium salt consisting of fatty acids with different carbon chains in parts by weight as follows:

fatty acids	FAC (%) soap index
		Octa acid	0.2-0.5
Decanoic acid	0.3-0.7
		Dodecanoic acid	5-8
Myristic acid	4-6
		Hexadecanoic acid	38-43
Octadecaenoic acid	36-41
		Stearic acid	5-8

Further, the herbal extract-purified fraction is obtained by the following method:

(1) extraction of

Lotus leaves, ligusticum, agastache and clove are mixed according to the weight ratio of (5-15): (10-25): (10-25): (15-35), decocting in water for 2 times, wherein the water added for the first time is 8-12 times of the total weight of the four components, decocting for 0.5-2 hours, the water added for the second time is 6-10 times of the total weight of the four components, decocting for 0.1-1 hour, filtering, combining filtrates, and concentrating under normal pressure to 0.5-2 g crude drug/mL for later use;

(2) purification of

And (2) adding ethanol with the weight being 1-3 times that of the water extract concentrated solution obtained in the step (1), stirring while adding, standing for 10-15 hours, separating supernatant, concentrating under reduced pressure until no alcohol smell exists, and drying in vacuum at 50-80 ℃ to obtain the antibacterial medicinal soap composition.

The medicated soap contains 0.1-2 wt% of flavonoids, 0.1-2 wt% of triterpenoids, 0.1-2 wt% of 10-octacosanol and 0.1-2 wt% of β -sitosterol.

The medicated soap contains 0.1-2 wt% of butylphthalide, 0.1-2 wt% of ligusticum wallichii triterpene and 0.1-2 wt% of octadecadienoic acid.

The medicated soap contains 0.1-2 wt% of monoterpene, 0.1-2 wt% of sesquiterpene, 0.1-2 wt% of patchouli alcohol and 0.1-2 wt% of D-guaiene.

The medicated soap contains 0.01-0.2 wt% of eugenol and 0.02-0.2 wt% of caryophyllene.

The invention also provides a preparation method of the mite-repelling and mite-killing antioxidant medicinal soap, and the mite-repelling and mite-killing antioxidant medicinal soap is prepared by adding herbal extracted and purified components into a soap base.

The invention relates to a skin-refreshing prescription which is prepared by combining four medicines of lotus leaf, ligusticum, wrinkled gianthyssop herb and clove and adding the four medicines into a conventional medicated soap base to prepare a medicated soap containing traditional Chinese medicine components, wherein the four medicines of the skin-refreshing prescription respectively belong to heat-clearing and fire-purging herbs, wind-cold-dispersing herbs, dampness-resolving herbs and interior-warming herbs in the traditional Chinese medicine classification. In the formula, lotus leaves are used as monarch drugs, and the monarch drugs are selected to grow hair, clear yang, cool and detoxicate; the ligusticum is used as a ministerial drug, and is aromatic to eliminate dampness, dispel wind and dredge collaterals; the agastache rugosus and the clove rosin are added, so that the aromatic body of the composition is fragrant, can avoid foul smell, can eliminate dampness and turbidity, and can beautify the skin. The four medicines have the antibacterial curative effect, and the ligusticum and the clove have the anti-inflammatory effect, so that the medicated soap prepared from the four medicines has the possible good effects of resisting bacteria, inhibiting bacteria, resisting inflammation, resisting virus, expelling acarid, killing acarid, resisting oxidation and the like.

The following are descriptions of four drugs respectively:

1. lotus leaf

The lotus leaf is dry leaf of Nelumbo nucifera Gaertn, collected in summer and autumn, sun-dried to seven-eight dry, removed leaf stalk, folded into semicircle or fan shape, dried, bitter, neutral, entered liver, spleen and stomach channels, heat-clearing and dampness-resolving, ascending and yang-clearing, blood-cooling and bleeding-stopping, used for summer-heat polydipsia, summer-heat damp diarrhea, spleen deficiency diarrhea, blood heat hematemesis and epistaxis, hematochezia metrorrhagia and metrostaxis, and folium Nelumbinis charcoal astringes to remove blood stasis and stops bleeding, and used for hemorrhage and postpartum anemic fainting.

The folium Nelumbinis mainly contains alkaloids, flavonoids, triterpenes, steroids, polyphenols, etc., including 15 flavones, 2 alkaloids, 2 steroids, 2 steroidal glycosides, 3 fatty alcohols, 1 phenol and 2 megastigmane glycosides, 10-octacosanol, β -sitosterol, 1-undecanol, 1-eicosanol, daucosterol, 6'-hydroxy-4, 4' -dimethoxychalcone, 1-hydroxy-3, 7, 8-trimethoxyketone, rhamnosine-3-O- β -D-glucopyranoside, chryseriol-7-O- β 0-D-glucopyranoside, quercetin-3-O- β 2-D-glucopyranoside, quercetin-3-O- β -L-rhamnopyranoside, hyperoside, quercetin-3-O-6342-D-isoglucose-3-O- α -D-3-O-6857-D-isoglucose → 2-O-3-O-468-D-rhamnoide, quercetin-3-O-3-O-D-3-D-glucopyranoside, quercetin-3-O-D-6342, quercetin-O-3-D-3-D-lauroside, rhamnoside, quercetin-3-O-3-D-6857-O-isoglucose → isoglucose-3-D-3-D-isoglucose → isoglucose-3-D → isoglucose-3-D → isoglucose-3-D → isoglucose-3-D-3-D-3-D.

The pharmacological actions of the lotus leaves are mainly as follows:

(1) antioxidant and antiaging effects

Different lotus leaf extracts have antioxidation, and the research on hydroxyl free radical (. OH) and hydroxyl free radical (. OH) of lotus leaf water extract (LLE) by adopting electron spin resonance method spin trapping technology in the prior artSuperoxide anion radical (O)^2-H.) shows that 26.94mg/ml-1 of LLE produces O to the hypoxanthine-xanthine oxidase system^2-The clearance rate of 65.6 percent, the total clearance of OH generated by the Fenten reaction system can be realized when the LLE concentration is more than 8.98mg/ml-1, and the clearance of O can be realized when the LLE with about 40mg/ml-1^2-It can be seen that the aqueous extract of lotus leaves is a better antioxidant, showing very strong antioxidant capacity at lower concentrations. The antioxidant effect of the lotus leaf flavone is measured and evaluated by a DPPH method, an iron thiocyanate (FTC) method and a thiobarbituric acid (TBA) method, and experimental results show that the lotus leaf flavone has good DPPH removing capacity, shows strong capacity of inhibiting linoleic acid oxidation in an automatic grease oxidation system, and has the concentration of 0.3mg/ml when the lotus leaf flavone is in the concentration of 0.3mg/ml^-1When the antioxidant is used, the antioxidant capacity is strongest and can be compared with 0.01 percent BHI.

(2) Bacteriostatic action

The lotus leaf extract has obvious bacteriostatic action, researches show that the lotus leaf ethanol extract has certain bacteriostatic action on penicillium, saccharomycetes, aspergillus niger and rhodotorula, and the bacteriostatic action is enhanced along with the increase of the concentration of the extract; the lotus leaf ethanol extract has different bacteriostatic effects on different bacteria under the same concentration, is sensitive to saccharomycetes and rhodotorula, has slightly inferior bacteriostatic effect on penicillium and aspergillus niger, and has the minimum bacteriostatic and bactericidal concentration of about 20-2000 mg/L on 4 bacteria^-1In the meantime.

2 Chinese ligusticum rhizome

Ligusticum sinense is dried rhizome and root of Ligusticum sinense Oliv. or Ligusticum sinense Nakai et Kitag, Umbelliferae, collected and dug when stem and leaf wither in autumn or seedling emergence in next spring, removed silt, dried in the sun or dried, pungent, warm, enter bladder channel, dispel wind, dispel cold, remove dampness, relieve pain, and can be used for treating common cold due to wind-cold, parietal pain, and rheumatalgia.

The rhizoma Ligustici mainly contains Z, Z ' -6, 6', 7,3 ' A dimeric ligustilide, rhizoma Ligustici Chuanxiong triterpene, octadecadienoic acid, sucrose, daucosterol, trans-ferulic acid and B-sitosterol, and also contains 1, 3-dilinolein, coniferyl aldehyde, myristic ether, stigmasterol, vanillin, pregnenolone, bergapten, xanthotoxin, indole-3-methyl formate, ferulic acid, (E) -3-methoxy-4, 5-methylenedioxy cinnamyl alcohol, p-hydroxybenzaldehyde (14), 3-methoxy-4, 5-methylenedioxy-acetophenone, α -ethoxymethyl-4-hydroxy-benzyl alcohol and the like

The pharmacological actions of ligusticum are mainly as follows:

(1) anti-inflammatory action

The 75% ethanol extract of ligusticum sinense is used for gastric lavage of mice by 5g crude drugs/kg and 15g crude drugs/kg, and the results show that the 75% ethanol extract of ligusticum sinense can obviously resist ear swelling of mice caused by dimethylbenzene, the average inhibition rate in 4 hours is respectively 26.9% and 32.4%, the acetic acid is also obviously inhibited to improve the permeability of capillary vessels in abdominal cavities of the mice, and in addition, the ethanol extract of ligusticum sinense and ligusticum liaotungensis also has obvious inhibition effect on carrageenan-type footpad swelling of the mice.

The ligusticum neutral oil is an effective part, the 1/10LD50 dose (equivalent to 7g of crude drug/kg) and the 1/5LD50 dose of the ligusticum neutral oil both remarkably resist the carrageenan plantar swelling of normal rats and rats with bilateral adrenals removed, also inhibit the dimethylbenzene ear swelling of mice, improve the capillary permeability of abdominal cavities of the mice by acetic acid and improve the capillary permeability of skin of the rats by histamine, but do not inhibit the chronic granulation hyperplasia of the rats caused by plastic rings and also do not prolong the survival time of the rats with the adrenals removed, and the ligusticum neutral oil has remarkable inhibitory action on the increase of the capillary permeability, inflammatory exudation and edema in the early stage of inflammation, has no relation with a pituitary-adrenals system and has no corticosteroid-like action. Butylphthalide (3-n-butylphthalide) is one of the anti-inflammatory active ingredients, and can inhibit neutrophil infiltration, myeloperoxidase activity and the expression of intercellular adhesion molecules and tumor necrosis factors in inflammatory areas, and inhibit the expression of arachidonic acid and phospholipase A2 genes released by tissues after ischemia.

(2) Mite-expelling and mite-killing function

The research shows that: the ligustilide acetone extract in the ligusticum has higher contact killing and fumigation activity on armyworm and 3-instar larvae of diamondback moth, and the contact killing LD50 in 24 hours is 0.0623mg dry sample/head and 0.1502mg dry sample/head respectively; the 12h fumigation LD50 is 24.3422mg dry sample/L and 10.6015mg dry sample/L respectively; has inhibitory effect on growth and development of yellow mealworm pupa, LD50 is 6.5157mg dry sample/head; after the dropping treatment, the poisoned armyworm shows symptoms such as excitation, spasm, paralysis and the like in sequence and finally dies; the inhibition of the growth and development of yellow mealworm pupae is similar to juvenile hormone activity.

3 Pogostemon cablin

Herba Agastaches is dry aerial part of herba Agastaches Pogostemon cablin (Blanco) Benth of Labiatae, is harvested when branches and leaves are flourishing, is dried in the sun and stuffy night, and is repeatedly dried, pungent and slightly warm, enters spleen, stomach and lung channels, is fragrant to resolve turbidity, regulates the middle warmer and stops vomiting, releases summer-heat, and is used for damp stagnation, epigastric fullness and vomiting, summer-heat and dampness exterior syndrome, early damp-warm, fever and fatigue, chest distress and discomfort, cold-damp retention summer-heat, abdominal pain and vomiting, and nasosinusitis headache.

Patchouli oil contains more monoterpene, sesquiterpene, alcohol, ketone, aldehyde and alkanoic acid compounds, wherein the mass fraction of more than 1% is 11, and the main components are as follows: patchouli alcohol, D-guaiene, A-bergamotene, erimophenol, B-guaiene, B-patchouli, trans-caryophyllene, and patchoulenone, patchouli ene, A-patchouli ene, cumarone, malarene, B-pinene, A-curzerene, C-apiene, A-pinene, D-elemene, etc.; alkaloids include patchouli pyridine, epiguaiadine, etc.; the flavonoid components are as follows: 5-hydroxy-7, 3 ', 4' -trimethoxyflavanone (I), 4', 5-dihydroxy-3, 3', 7-trimethoxyflavone (II), 3, 5-dihydroxy-7, 4 '-dimethoxyflavanone (III), 5-hydroxy-3, 7, 4' -trimethoxyflavone (IV), 5-hydroxy-3, 7,3 ', 4' -tetramethoxyflavone (V), 5,4 '-dihydroxy-3, 7, 3' -trimethoxyflavone (VI), 5, 4-dihydroxy-7-methoxyflavone (VII) and 3,5,7,3 ', 4' -pentahydroxyflavone (VIII), and phytolaccin, apigenin, rhamnan, 3-apigenin-7-glucoside, etc.; other components include eugenol, cinnamaldehyde, benzaldehyde, friedelin, epifrietol, oleanolic acid, B-sitosterol, daucosterol, etc.

The pharmacological actions of patchouli are mainly:

(1) antibacterial effect

Researches show that the patchouli oil has good specific and selective inhibition effect on dermatophytes, can completely inhibit the growth and reproduction of superficial fungi and sphagnum rubrum, microsporum canis, epidermophyton floccosum and the like, and has the Minimum Inhibitory Concentration (MIC) of 50-400 LL/L. Patchouli alcohol, isoguaiene and patchouli alkene are the most important compounds in the chemical components of patchouli oil, and are the key points of the antifungal effect of the patchouli oil, the patchouli essential oil has better inhibition effect on the growth of cryptococcus neoformans, chaetomium globosum and scoparia brevicaulis, the MIC of the patchouli essential oil is 0.15, 0.45 and 0.5mL/L in sequence, and the further experimental research finds that the patchoulione in the chemical components is one of the main antibacterial components, and the antibacterial activity of the A-guaiene is poor. When the concentration of the patchouli essential oil in the culture medium is 0.03-0.27%, the patchouli essential oil has inhibition effects on 13 tested common plant pathogenic bacteria to different degrees; when the action concentration is 0.1%, the inhibitor can inhibit the sandlewood hirsutella, the tomato early blight bacteria and the sclerotinia sclerotiorum.

(2) Anti-inflammatory action

In the prior art, a cell inflammation model and an animal inflammation model are adopted to carry out anti-inflammatory activity research on patchouli ketone and patchouli alcohol. The method comprises the steps of determining the influence of patchoulenone and patchoulol on excessive inflammatory mediators generated by macrophages induced by LPS by adopting an LPS-induced RAW264.7 macrophage inflammation model, and detecting the influence of the patchoulenone and the patchoulol on the expression of genes related to the inflammatory mediators. The results show that patchoulenone and patchoulol have inhibitory effect on cell inflammation induced by LPS, and the action routes of the patchoulenone and the patchoulol are probably that overproduction of inflammation mediators is inhibited by inhibiting the overexpression of induced inflammation mediator-related genes.

(3) Mite-expelling and mite-killing function

The indoor biological activity determination method is adopted to research the mite expelling and killing activities and active ingredients of the patchouli whole-grass essential oil, and the result shows that the patchouli essential oil and the patchouli ketone have better control effect on larvae of pieris rapae (L.) and diamondback moth (Plutella xylostella L.).

4 clove

The flos Caryophylli is the dried bud of Eugenia caryophyllata Thunb of Myrtaceae, and is picked up when the bud turns red from green, and sun-dried, and it is pungent and warm, and has effects of warming spleen, stomach, lung and kidney channels, warming middle-jiao and lowering adverse qi, invigorating kidney and tonifying yang, and has effects of spleen and stomach deficiency cold, singultus emesis, anorexia, vomiting and diarrhea, heart and abdomen psychroalgia, kidney deficiency and sexual impotence.

The clove volatile oil contains 29 compounds, which account for 98.43 percent of the total volatile oil and mainly comprise eugenol, caryophyllene, α -caryophyllene, Naphtalene, 1,2,3,5,6, sa-hexahydro-4,7-dimethyl-1- (1-methyphenyl) - (1.36 percent), 3 ', 4' -trimethoxyanetophenone (1.36 percent), phenyl, 2-methoxy-4- (2-propynyl) -aeetate (17.93 percent).

The pharmacological actions of clove are mainly as follows:

(1) antibacterial and anti-inflammatory effects

At present, researchers have separated 6 compounds from lilac leaves, namely D-mannitol, tyrosol, trans-p-hydroxycinnamic acid, 3, 4-dihydroxy phenylacetic acid, 3, 4-dihydroxy benzoic acid and syringopicroside, and studied the physiological activity of the compounds, and experiments show that: tyrosol, trans-p-hydroxycinnamic acid, 3, 4-dihydroxy phenylacetic acid, 3, 4-dihydroxy benzoic acid and syringopicroside aglycone have different degrees of inhibition effects on staphylococcus aureus, dysentery bacillus, escherichia coli and pseudomonas aeruginosa, wherein the activity of the 3, 4-dihydroxy phenethyl alcohol is strongest, the minimum inhibitory concentration of the 3, 4-dihydroxy phenethyl alcohol to dysentery bacillus is 6.25r/mL-1, the action of the clove in Guandong is similar to that of the lilac, the antibacterial experiment of the 3, 4-dihydroxy phenylethanol shows that the clove has obvious antibacterial action on dysentery bacillus, typhoid bacillus, paratyphoid bacillus A and B, food poisoning salmonella, staphylococcus aureus, common proteus, pseudomonas aeruginosa and the like, and the ethanol extract of the clove has obvious inhibition action on wheat rhizoctonia solani, cotton fusarium wilt, corn microsporum and citrus green mildew; in addition, the clove has good inhibition effect on penicillium cumulans, botrytis cinerea and apple brown mould, the clove has stronger inhibition effect on frailty bacilli, melanogenesis bacilli, digestive streptococcus and the like than on normal vaginal constant-value lactobacillus casei, and the clove has stronger inhibition effect and killing effect on shigella flexneri, salmonella, escherichia coli, pseudomonas aeruginosa, staphylococcus aureus and the like. Eugenol has the function of inhibiting lipoxygenase and cyclooxygenase, clove alcohol extract has the function of inhibiting white larynx bacillus, bacillus anthracis, paratyphoid bacillus, dysentery bacillus and cholera vibrio, clove extract has strong inhibiting effect on golden yellow staphylococcus, acid producing bacillus, hydrogen sulfide producing bacillus, cloaca bacillus and the like, and the antibacterial mechanism of the clove extract is related to the synthesis process of aminoglycoside competitive inhibition protease. Clove has broad-spectrum antibacterial action and obvious phlegm eliminating and inflammation diminishing effects, and the action mechanism of eugenol on fungi is similar to that of most antibacterial drugs, namely, eugenol acts on cell membranes of fungi to achieve the aim of killing the fungi. Experiments prove that: the clove preservative substance is stable to heat and can keep the bacteriostatic ability within 100e, so that the clove preservative substance can be used as a preservative for various cold and hot processed foods.

(2) Antiviral action

The capsule containing Syringa oblata as main component has the effect of inhibiting cytopathic effect of adenovirus, parainfluenza virus, respiratory syncytial virus and coxsackie virus, but has no obvious effect on cytopathic effect of enterovirus.

In the present invention, the quantitative analysis method of the main components in the herb extracts refers to the prior art method.

Compared with the prior art, the skin-refreshing prescription is prepared by combining four medicines of lotus leaf, ligusticum, wrinkled gianthyssop herb and clove into a skin-refreshing prescription, and adding the skin-refreshing prescription into a conventional medicated soap base to prepare the medicated soap containing traditional Chinese medicine components, wherein the four medicines of the skin-refreshing prescription respectively belong to heat-clearing and fire-purging herbs, wind-cold-dispersing herbs, dampness-resolving herbs and interior-warming herbs in the classification of traditional Chinese medicines. In the formula, lotus leaves are used as monarch drugs, and the monarch drugs are selected to grow hair, clear yang, cool and detoxicate; the ligusticum is used as a ministerial drug, and is aromatic to eliminate dampness, dispel wind and dredge collaterals; the agastache rugosus and the clove rosin are added, so that the aromatic body of the composition is fragrant, can avoid foul smell, can eliminate dampness and turbidity, and can beautify the skin. The four medicines have the antibacterial curative effect, and the ligusticum and the clove have the anti-inflammatory effect, so that the medicated soap prepared from the four medicines has the possible good effects of resisting bacteria, inhibiting bacteria, resisting inflammation, resisting virus, expelling acarid, killing acarid, resisting oxidation and the like.

The four medicines adopted by the invention have synergistic action with each other, wherein the lotus leaf extract contains flavonoid and triterpenes, so that the stability of the anti-inflammatory active ingredients of the butylphthalide (3-n-butylphthalide) in the ligusticum extract is promoted, and the terpenes of the agastache rugosus and clove extract are subjected to synergistic action with or have synergistic action with the terpenes of the agastache rugosus and clove extract, so that the antibacterial, bacteriostatic, anti-inflammatory, antiviral, acarid-expelling, acarid-killing, antioxidant and other effects of the medical soap are improved.

Detailed Description

The present invention will be described in detail with reference to specific examples.

The soap base used in the following examples is a commercially available soap base. The content of fatty acid in the soap base is more than or equal to 78 percent, the content of free acid (calculated by oleic acid) is less than or equal to 0.8 percent, the content of chloride (calculated by NaCL) is less than or equal to 0.7 percent, the content of ethanol insoluble substances is less than or equal to 2 percent, and the content of water and volatile substances is less than or equal to 14 percent; the fatty acid solidification point is 39-43 ℃.

Example 1

The anti-oxidation medical soap for expelling and killing mites contains 1% of herbal extraction and purification components in the soap base, the herbal extraction and purification components are extracts of skin reviving formulas consisting of four herbal raw materials and purified products, and the skin reviving formulas are lotus leaves, ligusticum sinense, wrinkled gianthyssop herb and clove according to the weight ratio of 5: 10: 10: 10 by weight.

The herbal extract is prepared by the following steps:

(1) extraction of

Mixing lotus leaf, ligusticum, agastache and clove according to the weight ratio of 5: 10: 10: 10, adding water for decoction, extracting for 2 times, wherein the water added for the first time is 10 times of the total weight of the three components, decocting for 1 hour, the water added for the second time is 8 times of the total weight of the three components, decocting for 0.5 hour, filtering, combining filtrates, and concentrating under normal pressure to 1g crude drug/mL for later use;

(2) purification of

Adding 2 times of ethanol into the water extraction concentrated solution obtained in the step (1), stirring while adding, standing for 12 hours, separating supernatant, concentrating under reduced pressure until no ethanol smell exists, and vacuum drying at 60 ℃ to obtain the herbal extracted and purified component.

And adding the herbal extracted and purified components into a soap base to prepare the anti-acarid and acarid-killing antioxidant medicated soap.

Example 2

The anti-oxidation medical soap for expelling and killing mites contains 0.3 weight percent of herbal extraction and purification components in a soap base, the herbal extraction and purification components are extracts of skin reviving formulas consisting of four herbal raw materials and purified products, and the skin reviving formulas are lotus leaves, ligusticum sinense, agastache rugosus and clove according to the ratio of 3: 5: 5: 7 in a weight ratio.

The herbal extract is prepared by the following steps:

(1) extraction of

Mixing lotus leaf, ligusticum, agastache and clove according to the weight ratio of 3: 5: 5: 7, adding water for decoction, extracting for 2 times, wherein the water added for the first time is 8 times of the total weight of the three components, decocting for 0.5 hour, the water added for the second time is 6 times of the total weight of the three components, decocting for 0.1 hour, filtering, combining filtrates, and concentrating under normal pressure to 0.5g crude drug/mL for later use;

(2) purification of

Adding 1 time of ethanol into the water extraction concentrated solution obtained in the step (1), stirring while adding, standing for 10 hours, separating supernatant, concentrating under reduced pressure until no ethanol smell exists, and vacuum drying at 50 ℃ to obtain the herbal extracted and purified component.

Example 3

The anti-oxidation medical soap for expelling and killing mites contains 2% of herbaceous extraction and purification components in the soap base, the herbaceous extraction and purification components are extracts of four herbal raw materials and purified products after the extracts are purified, and the skin-refreshing formulas are lotus leaves, ligusticum sinense, wrinkled gianthyssop herb and clove according to the weight ratio of 10: 15: 15: 15 in a weight ratio.

The herbal extract is prepared by the following steps:

(1) extraction of

Lotus leaf, ligusticum, wrinkled gianthyssop herb and clove are mixed according to the weight ratio of 10: 15: 15: 15, adding water, decocting for 2 times, wherein the water amount added for the first time is 12 times of the total weight of the three components, decocting for 2 hours, the water amount added for the second time is 10 times of the total weight of the three components, decocting for 1 hour, filtering, combining filtrates, and concentrating under normal pressure to 2g crude drug/mL for later use;

(2) purification of

Adding 3 times of ethanol by weight into the water extraction concentrated solution obtained in the step (1), stirring while adding, standing for 15 hours, separating supernatant, concentrating under reduced pressure until no ethanol smell exists, and vacuum drying at 80 ℃ to obtain the herbal extracted and purified component.

Example 4

The anti-oxidation medical soap for expelling and killing mites contains 1.5 weight percent of herbal extraction and purification components in a soap base, the herbal extraction and purification components are extracts of skin reviving formulas consisting of four herbal raw materials and purified products, and the skin reviving formulas are lotus leaves, ligusticum sinense, agastache rugosus and clove according to the ratio of 6: 8: 10: 12 by weight.

The herbal extract is prepared by the following steps:

(1) extraction of

Mixing lotus leaf, ligusticum, agastache and clove according to the weight ratio of 6: 8: 10: 12, adding water, decocting for 2 times, wherein the water amount added for the first time is 9 times of the total weight of the three components, decocting for 1.5 hours, the water amount added for the second time is 7 times of the total weight of the three components, decocting for 0.8 hours, filtering, combining filtrates, and concentrating under normal pressure to 1.5g crude drug/mL for later use;

(2) purification of

Adding ethanol with the weight of 1.5 times of the water extraction concentrated solution obtained in the step (1), stirring while adding, standing for 11 hours, separating supernatant, concentrating under reduced pressure until no alcohol smell exists, and vacuum drying at 65 ℃ to obtain the herbal extracted and purified component.

Example 5

The anti-oxidation medical soap for expelling and killing mites contains 0.5 weight percent of herbal extraction and purification components in a soap base, the herbal extraction and purification components are extracts of skin reviving formulas consisting of four herbal raw materials and purified products, and the skin reviving formulas are lotus leaves, ligusticum sinense, agastache rugosus and clove according to the ratio of 8: 12: 12: 8 in a weight ratio.

The herbal extract is prepared by the following steps:

(1) extraction of

Mixing lotus leaf, ligusticum, agastache and clove according to the weight ratio of 8: 12: 12: 8, adding water for decoction, extracting for 2 times, wherein the water added for the first time is 11 times of the total weight of the three components, decocting for 0.8 hour, the water added for the second time is 8 times of the total weight of the three components, decocting for 0.4 hour, filtering, combining filtrates, and concentrating under normal pressure to 0.7g crude drug/mL for later use;

(2) purification of

Adding 2.5 times of ethanol into the water extraction concentrated solution obtained in the step (1), stirring while adding, standing for 14 hours, separating supernatant, concentrating under reduced pressure until no alcohol smell exists, and vacuum drying at 75 ℃ to obtain the herbal extracted and purified component.

Control group: dermatitis treating medicine

The medicated soaps prepared from the compositions prepared in examples 1-5 were subjected to the following test experiments to test their performance:

1. sterilizing and itching relieving performance

(1) Influence on itch caused by histamine phosphate

50 Kunming mice with weight of 20 +/-2 g and half each male and female were randomly divided into 5 groups, and the compositions obtained in examples 1 to 5 were tested by using models

The skin of the right hind paw of the mouse was polished with fine sandpaper, 0.02% histamine phosphate 0.1mL was dropped on the polished skin to the extent that the skin became red and bled without bleeding, and the number of times and duration of itching of the mouse within 15min were immediately observed and recorded. The spasmolytic indication is that the mouse returns to lick and gnaw the back and instep skin; the interval of the pruritus duration time is more than 3 seconds and is recorded as two pruritus, and the interval of the pruritus duration time is less than 3 seconds and is recorded as one pruritus. Immediately after molding, the composition prepared in each example was uniformly applied to the skin of the right hind paw of a mouse and gently rubbed for a moment, and the number and duration of itching of the mouse were observed and recorded within 15min, and the results are shown in table 1.

TABLE 1 antipruritic effect on histamine phosphate-induced pruritus

	Number of animals	Number of itching (times)	Duration (min)
				Example 1	10	10.4	100.1
Example 2	10	13.3	120.1
				Example 3	10	12.1	119.3
Example 4	10	12.7	115.4
				Example 5	10	11.6	112.9
Control group	10	12.6	105.3

The results show that the antipruritic cream has a remarkable antipruritic effect when being smeared on the skin of a pruritus model mouse, the effect is close to that of a positive drug control group, and the antipruritic effect is exact.

2. Sterilizing effect of medicated soap

According to the evaluation method of the antibacterial and bacteriostatic effects of QBT 2738-:

TABLE 2 bacteriostatic effect

The result shows that the invention has excellent antibacterial and bacteriostatic rate. The control group has low significant antibacterial and bacteriostatic rate.

3. Mite-expelling and mite-killing effect

Table 3 the mite-repellent and mite-exterminating effects are measured according to the industry standard NY/T1151.2-2006 as detailed in table 3 below:

the result shows that the invention has excellent mite-expelling and mite-killing effects. The control group had significant acarid-repelling and acarid-killing effects.

4. Antioxidant effect

Determination of DPPH free radical eliminating capacity and SOD superoxide dismutase antioxidation capacity

Solution preparation:

weighing respectively:

VC 27.24, 49.41 and 99.11mg are added into 50ml of water;

examples 152.03, 99.35mg constant volume to 50ml water;

examples 250.49, 100.91mg constant volume to 50ml water;

examples 349.69, 104.2mg to 50ml water;

examples 450.71, 104.35mg were made up to 50ml of water.

Examples 548.51, 103.32mg were made up to 50ml of water.

Preparing a DPPH solution:

accurately weighing 4.91mg of DPPH into a 100mL volumetric flask, and using absolute ethyl alcohol to fix the volume to obtain 49.1mg/L DPPH mother liquor.

The determination method comprises the following steps:

2.0mL of each sample solution is respectively added into a 10mL colorimetric tube, and then 2.0mL of the PPH solution is parallelly added into each colorimetric tube. Control and blank groups were set simultaneously. And (4) lightly oscillating to fully and uniformly mix the mixture, and reacting the colorimetric tube in the dark for 30min to ensure that the medicine completely reacts with the free radicals. Measured at a wavelength of 517nm and the final A value is recorded. And (4) zero setting by taking absolute ethyl alcohol as a reference. The DPPH clearance (I) for each sample was calculated according to the following formula.

I/％＝〔1－(A_i－A_j)/A₀〕×100

In the formula A₀Absorbance of unpelled DPPH (2.0mL DPPH +2.0mL solvent), absorbance of Ai after reaction of sample with DPPH, and absorbance of Aj for sample blank (2.0mL95 ethanol +2.0mL sample). To reduce experimental error, 3-6 cuvettes were averaged for each sample.

TABLE 4 results of the experiment

Sample (I)	DPPH radical clearance%
		VC(0.5448mg/ml)	95.46
VC(0.9882mg/ml)	95.88
		VC(1.9822mg/ml)	95.67
Example 1(1.0406mg/ml)	32.53
		Example 2(1.9870mg/ml)	36.87
Example 2(1.0098mg/ml)	39.47
		Example 3(2.0042mg/ml)	38.96
Example 3(0.9938mg/ml)	36.48
		Example 4(1.0142mg/ml)	47.25
Example 4(2.0870mg/ml)	50.96
		Example 5(0.9702mg/ml)	53.95
Example 5(2.0664mg/ml)	52.97
		Blank control group	0

As a result, the present invention has an excellent effect of oxidation resistance. The control group had significantly no effect.

The embodiments described above are described to facilitate an understanding and use of the invention by those skilled in the art. It will be readily apparent to those skilled in the art that various modifications to these embodiments may be made, and the generic principles described herein may be applied to other embodiments without the use of the inventive faculty. Therefore, the present invention is not limited to the above embodiments, and those skilled in the art should make improvements and modifications within the scope of the present invention based on the disclosure of the present invention.

Claims

1. The anti-oxidation medical soap for expelling and killing mites is characterized in that a soap base of the medical soap contains 0.3-2% of herbal extraction and purification components in weight proportion, the herbal extraction and purification components are extracts and purified products of a skin-refreshing formula consisting of four herbal raw materials, and the skin-refreshing formula is lotus leaves, ligusticum sinense, agastache rugosus and clove according to the ratio of (5-15): (10-25): (10-25): (15-35) in terms of weight ratio.

2. The anti-acarid and acarid-killing antioxidant medicated soap as claimed in claim 1, wherein the soap base is a commercially available soap base, the content of fatty acid is not less than 78%, the content of free acid (calculated as oleic acid) is not more than 0.8%, the content of chloride (calculated as NaCL) is not more than 0.7%, the content of ethanol insoluble substance is not more than 2%, and the content of water and volatile substance is not more than 14%; the fatty acid solidification point is 39-43 ℃.

3. The anti-acarid, acaricidal and antioxidant medicated soap of claim 1, wherein said herbal extract purified ingredients are obtained by the following method:

(1) extraction of

(2) purification of

And (2) adding ethanol with the weight being 1-3 times that of the water extract concentrated solution obtained in the step (1), stirring while adding, standing for 10-15 hours, separating supernatant, concentrating under reduced pressure until no alcohol smell exists, and drying in vacuum at 50-80 ℃ to obtain the antibacterial and anti-inflammatory medicinal soap composition.

4. The anti-mite and anti-oxidation medicated soap as claimed in claim 1, which is characterized by comprising 0.1-2 wt% of flavonoids, 0.1-2 wt% of triterpenoids, 0.1-2 wt% of 10-octacosanol and 0.1-2 wt% of β -sitosterol.

5. The anti-acarid and acarid-killing antioxidant medical soap as claimed in claim 1, wherein the medical soap comprises 0.1-2 wt% of butylphthalide, 0.1-2 wt% of ligusticum wallichii triterpene and 0.1-2 wt% of octadecadienoic acid.

6. The anti-acarid and acaricidal antioxidant medical soap as claimed in claim 1, which comprises monoterpene 0.1-2 wt%, sesquiterpene 0.1-2 wt%, patchouli alcohol 0.1-2 wt%, and D-guaialene 0.1-2 wt%.

7. The anti-acarid and acaricidal antioxidant medicated soap as claimed in claim 1, which comprises 0.1-2 wt% of eugenol and 0.1-2 wt% of caryophyllene.

8. The method for preparing the acaricidal and acaricidal antioxidant medicinal soap according to any one of claims 1 to 7, which is characterized in that the acaricidal and acaricidal antioxidant medicinal soap is prepared by adding herbal extracted and purified ingredients to a soap base.