CN110856824A

CN110856824A - Micro-fluidic chip

Info

Publication number: CN110856824A
Application number: CN201810960650.9A
Authority: CN
Inventors: 闵小平; 葛胜祥; 张师音; 张东旭; 苏晓崧; 付达; 翁振宇; 陈文堤; 翁祖星; 宋浏伟; 张军; 夏宁邵
Original assignee: Xiamen Wantai Kairui Biological Technology Co Ltd; Xiamen University
Current assignee: Xiamen Wantai Kairui Biological Technology Co Ltd; Xiamen University
Priority date: 2018-08-22
Filing date: 2018-08-22
Publication date: 2020-03-03

Abstract

The invention relates to a micro-fluidic chip which comprises a chip body and a sliding block. The chip body comprises more than two chip flow channels which are not communicated with each other; the slide block and the chip body can be arranged in a relatively sliding mode, and at least one slide block flow channel is arranged in the slide block; the sliding of the slider relative to the chip body enables the slider flow channel to be communicated with different chip flow channels so as to form a flow path between the different chip flow channels. The micro-fluidic chip structure can meet the control requirements of various flow paths, has simple and reliable control method, can meet the requirement of batch manufacturing and has low cost.

Description

Micro-fluidic chip

Technical Field

The invention relates to the technical field of microfluidics, in particular to a microfluidic chip.

Background

The microfluidic chip is a technology for processing pipelines and reaction chambers on the chip and driving reagents to flow in the pipelines and the chambers so as to complete various biological and chemical processes. In recent years, micro-fluidic chips are rapidly developed in the direction of functionalization and integration, and important biological and chemical processes such as nucleic acid amplification reaction and immune reaction become new hot spots. To complete these complicated testing processes, switching among multiple flow paths, and opening and closing the flow paths multiple times must be performed on the chip, which requires a simple and reliable valve. On the micro-fluidic chip, the micro-fluidic chip is influenced by factors such as processing difficulty, cost, volume, reliability and the like, and the micro-fluidic chip is easy to manufacture, simple, reliable, low in cost and suitable for batch control valves, and is always a difficult problem.

Disclosure of Invention

The invention aims to provide a microfluidic chip, which can meet the control requirements of various flow paths in structure, has a simple and reliable control method, can meet the requirements of batch manufacturing and is low in cost.

The invention relates to a microfluidic chip comprising:

the chip comprises a chip body, wherein more than two chip flow channels which are not communicated with each other are arranged in the chip body; and

the sliding block and the chip body are arranged on the chip body in a relatively sliding mode, and a sliding block flow channel is arranged in the sliding block; the sliding of the slider relative to the chip body enables the slider flow channel to be communicated with different chip flow channels so as to form a flow path between the different chip flow channels.

Further, the slide block slides relative to the chip body to enable one slide block flow passage to be communicated with the two chip flow passages, so that a flow path is formed between the two chip flow passages.

Further, the flow path is a non-branching line type flow path.

Furthermore, the slider comprises a plurality of slider runners, at most only one slider runner is communicated with at least two chip runners in the relative sliding process of the slider and the chip body, and the chip runners communicated with different slider runners are not identical.

Furthermore, the microfluidic chip also comprises a chip covering layer which is arranged between the sliding block and the chip body and is positioned on the surface of the chip body, a through hole corresponding to the open hole of the chip flow channel positioned on the surface of the chip body is arranged on the chip covering layer, and the covering layer is used for lubricating the sliding block and/or sealing the gap between the sliding block flow channel and the chip flow channel; and/or the microfluidic chip further comprises a slider covering layer arranged between the slider and the chip body and positioned on the surface of the slider, through holes corresponding to the open holes of the slider flow channels positioned on the surface of the slider are formed in the slider covering layer, and the slider covering layer is used for lubricating the slider and/or sealing gaps between the slider flow channels and the chip flow channels.

Further, the material of the chip covering layer or the sliding block covering layer is polytetrafluoroethylene and/or fluorinated ethylene propylene.

Furthermore, the surface of the sliding block is provided with a positioning part corresponding to the position of the sliding block flow channel.

Furthermore, the positioning part comprises a bulge which is in one-to-one correspondence with the slide block flow channel.

Further, the slider includes the slider upper strata and with the slider lower floor that slider upper strata bottom is connected, open the slider upper strata bottom has the bar groove, the slider lower floor seted up with two through-holes that the bar groove is linked together, two through-holes with the bar groove forms jointly the slider runner and two through-holes form the exit of slider runner.

Furthermore, the micro-fluidic chip also comprises a compressing structure, the compressing structure is fixedly connected with the chip body, and the compressing structure compresses the sliding block on the surface of the chip body. Further, an anti-friction structure for reducing friction between the compression structure and the sliding block is arranged between the compression structure and the sliding block.

Further, the antifriction structure comprises an arc-shaped bulge or a self-lubricating material layer arranged on the contact part of the pressing structure or the sliding block.

Further, the antifriction structure comprises a rolling body arranged between the pressing structure and the sliding block.

Further, antifriction structure includes the holder, the holder set up in compact structure or between the slider, the rolling element with be located on the holder and can rotate relatively the holder.

Further, the compression structure includes:

a first guide portion for defining a sliding direction of the slider; and/or

A first stopper for defining a sliding distance of the slider.

Further, the pressing structure comprises a top plate, the sliding block is slidably disposed between the top plate and the chip body, the top plate comprises an opening or a sliding groove in sliding fit with the top of the sliding block, and the inner wall of the opening or the sliding groove forms the first guide portion and/or the first stopping portion.

Furthermore, a reinforcing plate is arranged on one side, away from the sliding block, of the chip body, the microfluidic chip comprises a reinforcing structure arranged on one side, away from the sliding block, of the chip body, and the reinforcing structure is used for reducing bending deformation of the chip body towards one side, away from the sliding block.

Further, the microfluidic chip further comprises:

a second guide portion provided on the chip body, the second guide portion defining a sliding direction of the slider; and/or the presence of a gas in the gas,

the second stopping part is arranged on the chip body, and the second stopping part limits the relative sliding distance between the sliding block and the chip body.

Further, the second guide portion comprises two first bar-shaped blocks arranged in parallel at intervals, the second stopping portion comprises two second bar-shaped blocks arranged in parallel at intervals and perpendicular to the two first bar-shaped blocks, and the two first bar-shaped blocks and the two second bar-shaped blocks form a containing space for containing the bottom of the sliding block.

Further, the material of the sliding block is plastic or rubber.

Based on the micro-fluidic chip provided by the invention, the connection and disconnection switching among a plurality of flow paths of the micro-fluidic chip can be realized by arranging the sliding block, the structure is simple and reliable, the control method is simple, the processing and the manufacturing are easy, the micro-fluidic chip is suitable for batch production, and the cost is low.

Other features of the present invention and advantages thereof will become apparent from the following detailed description of exemplary embodiments thereof, which proceeds with reference to the accompanying drawings.

Drawings

The accompanying drawings, which are included to provide a further understanding of the invention and are incorporated in and constitute a part of this application, illustrate embodiment(s) of the invention and together with the description serve to explain the invention without limiting the invention. In the drawings:

fig. 1 is a schematic structural diagram of a microfluidic chip according to an embodiment of the present invention;

FIG. 2 is a cross-sectional structural view of the slider shown in FIG. 1;

FIG. 3 is a schematic diagram of a slider runner of a slider and a chip runner of a chip body in a disconnected state according to another embodiment of the present invention;

FIG. 4 is a schematic diagram of a slider runner of the slider shown in FIG. 3 in a communicating state with a chip runner of a chip body;

FIG. 5 is a schematic view of a slider runner structure of a slider according to yet another embodiment of the present invention;

FIG. 6 is a schematic diagram of a chip flow channel of the chip body in the embodiment shown in FIG. 5;

FIG. 7 is a schematic view of a slider runner and a chip runner communicating structure of the slider in the embodiment of FIG. 5;

FIG. 8 is a schematic view of another slider runner and chip body chip runner communication structure of the slider in the embodiment shown in FIG. 5;

FIG. 9 is a schematic view of a further slider runner and chip body chip runner communication structure of the slider of the embodiment shown in FIG. 5;

fig. 10 is a schematic structural diagram of a microfluidic chip according to another embodiment of the present invention;

fig. 11 is a schematic structural diagram of a microfluidic chip according to another embodiment of the present invention;

FIG. 12 is a schematic structural diagram illustrating a positional relationship between a positioning portion of the slider and a slider channel in the embodiment shown in FIG. 11.

Detailed Description

The technical solutions in the embodiments of the present invention will be clearly and completely described below with reference to the drawings in the embodiments of the present invention, and it is obvious that the described embodiments are only a part of the embodiments of the present invention, and not all of the embodiments. The following description of at least one exemplary embodiment is merely illustrative in nature and is in no way intended to limit the invention, its application, or uses. All other embodiments, which can be derived by a person skilled in the art from the embodiments given herein without making any creative effort, shall fall within the protection scope of the present invention.

The relative arrangement of the components and steps, the numerical expressions and numerical values set forth in these embodiments do not limit the scope of the present invention unless specifically stated otherwise. Meanwhile, it should be understood that the sizes of the respective portions shown in the drawings are not drawn in an actual proportional relationship for the convenience of description. Techniques, methods, and apparatus known to those of ordinary skill in the relevant art may not be discussed in detail but are intended to be part of the specification where appropriate. In all examples shown and discussed herein, any particular value should be construed as merely illustrative, and not limiting. Thus, other examples of the exemplary embodiments may have different values. It should be noted that: like reference numbers and letters refer to like items in the following figures, and thus, once an item is defined in one figure, further discussion thereof is not required in subsequent figures.

Spatially relative terms, such as "above … …," "above … …," "above … …," "above," and the like, may be used herein for ease of description to describe one device or feature's spatial relationship to another device or feature as illustrated in the figures. It will be understood that the spatially relative terms are intended to encompass different orientations of the device in use or operation in addition to the orientation depicted in the figures. For example, if a device in the figures is turned over, devices described as "above" or "on" other devices or configurations would then be oriented "below" or "under" the other devices or configurations. Thus, the exemplary term "above … …" can include both an orientation of "above … …" and "below … …". The device may be otherwise variously oriented (rotated 90 degrees or at other orientations) and the spatially relative descriptors used herein interpreted accordingly.

As shown in fig. 1, 10, and 11, the present embodiment discloses a microfluidic chip, which includes a chip body 10 and a slider 20. The chip body 10 includes more than two chip flow channels that are not communicated with each other. The slider 20 and the chip body 10 are slidably disposed relative to each other, and a slider flow path is included in the slider 20. The sliding of the slider 20 relative to the chip body 10 enables the slider flow channel to communicate with different chip flow channels to form a flow path therebetween.

For example, as shown in fig. 3 and 4, the slider 20 may include one slider flow path u for communicating with and interrupting two chip flow paths of the chip body 10, i.e., a first chip flow path a and a second chip flow path b.

The micro-fluidic chip disclosed in this embodiment can realize switching between connection and disconnection of a plurality of flow paths on the micro-fluidic chip by arranging the slider 20, and is simple and reliable. When switching between the connected and disconnected states, only an external force, such as a pushing or pulling force applied by the driving device, is required to act on the corresponding surface of the slider 20 for driving. In the case where the driving device is provided, the relative position between the driving device and the slider 20 is less required to be positioned. The microfluidic chip of the embodiment has the advantages of simple control method for switching the flow path state, easy processing and manufacturing, suitability for batch production and low cost.

In some embodiments, as also shown in fig. 5, 6, 7, and 8, the slider 20 may include a plurality of slider runners, such as the illustrated first slider runner u, second slider runner v, and third slider runner w, for respectively communicating with and disconnecting from two of the first chip runner a, the second chip runner b, the third chip runner c, and the fourth chip runner d of the chip body.

In some embodiments, the slider 20 may further include a plurality of slider runners, and two or more slider runners are simultaneously connected to and disconnected from the plurality of chip runners in the chip body to simultaneously connect and disconnect the plurality of flow paths isolated from each other or the trunk and the plurality of branch flow paths.

In some embodiments, as shown in fig. 3, 7, 8 and 9, slider 20 slides relative to die body 10 to place one slider runner in communication with two die runners to form a flow path between the two die runners. In the embodiment, two ends of the slide block flow channel are respectively communicated with one chip flow channel, and the slide block flow channel has no branch flow path, so that fluid always flows along one flow path when flowing in the flow channel, the problem of fluid residue at a branch port when the flow path is branched is avoided, and the reliability of fluid reaction of the microfluidic chip is improved. In some embodiments, the slider flow channel and the chip flow channel are non-branching linear flow channels, and the formed flow channel formed by the slider flow channel and the chip flow channel is a non-branching linear flow channel. This arrangement further avoids the problem of fluid residue at the bifurcation.

In some embodiments, the slider 20 includes a plurality of slider runners, and during the relative sliding between the slider 20 and the chip body 10, at most only one slider runner communicates with at least two chip runners, so as to form a flow path between at least two chip runners, and the chip runners communicated by different slider runners are not identical.

As shown in fig. 7, 8, and 9, when one of the first, second, and third slider flow paths u, v, and w forms a flow path with at least two chip flow paths among the first, second, third, and fourth chip flow paths a, b, c, and d, the remaining two slider flow paths do not form a flow path, and the flow paths formed by each slider flow path are different from each other.

In this embodiment, the slider 20 has a plurality of slider channels, so that a function of switching a plurality of channels of the microfluidic chip can be realized, meanwhile, each chip channel corresponds to one channel, the communication and the switching are safe and reliable, and the slider 20 can realize the switching among different channels through one-way movement, thereby being beneficial to reducing the difficulty in designing the channels of the microfluidic chip and improving the designability.

In some embodiments, as shown in fig. 10 and 11, the microfluidic chip further includes a chip cover layer 50 disposed between the slider 20 and the chip body 10 on the surface of the chip body 10, the chip cover layer 50 is provided with a through hole corresponding to the opening of the chip flow channel on the surface of the chip body 10, and the chip cover layer 50 is used for lubricating the sliding of the slider 20 and/or sealing the connection and disconnection between the slider flow channel and the chip flow channel. The chip cover layer 50 may be fixed on the surface of the chip body 10 by chemical bonding, thermal pressing or double-sided adhesive bonding. The material used for the chip covering layer 50 does not react with the reagent, and when the material selected for use for the chip covering layer 50 has a sealing effect, the chip covering layer 50 has certain elasticity, and can deform to a certain extent when the sliding block 20 is pressed down, so that the chip covering layer 50 can eliminate a gap between the chip covering layer and the contact surface of the sliding block 20, and a better sealing effect is achieved. When the chip cover layer 50 is used for lubricating the slider 20, the cover layer 50 is selected so that the slider 20 can slide more smoothly.

In some embodiments, the microfluidic chip further includes a slider cover layer disposed between the slider 20 and the chip body 10 on the surface of the slider 20, the slider cover layer having a through hole corresponding to the opening of the slider channel on the surface of the slider 20, the slider cover layer being configured to lubricate the slider and/or seal the gap between the slider channel and the chip channel.

In some embodiments, the material of the chip cover layer 50 or the slider cover layer is polytetrafluoroethylene and/or perfluoroethylene propylene. The chip cover layer 50 or the slider cover layer made of the material does not react with the reagent, and in addition, the material can simultaneously have a better lubricating effect on the slider 20 and a function of sealing a gap between the slider flow channel and the chip flow channel.

In some embodiments, as shown in fig. 11 and 12, the surface of the slider 20 is provided with a positioning portion corresponding to the position of the slider flow channel. According to the arrangement, when the sliding block 20 slides, the position of the positioning parts is detected by sensors such as a photoelectric switch, and the position of the sliding block flow channel of the sliding block 20 can be determined, so that whether the sliding block flow channel is correctly communicated with the chip flow channel or not can be judged, the detectability of the sliding block flow channel is improved, and the accuracy and the reliability of the microfluidic chip are improved.

In some embodiments, as shown in fig. 11 and 12, the positioning portion includes protrusions 23 corresponding to the slider runners one to one. The bulges 23 which are in one-to-one correspondence with the slide block flow channels are arranged on the side surfaces of the slide block 20, so that the positions of the slide block flow channels 23 are more visual, and the detection of the positions of the slide block flow channels 23 is further facilitated.

In some embodiments, as shown in fig. 1, fig. 2, fig. 10, and fig. 11, the slider 20 includes an upper slider layer 21 and a lower slider layer 22 connected to the bottom of the upper slider layer 21, the bottom of the upper slider layer is provided with a strip-shaped groove 211, the lower slider layer 22 is provided with two through holes 221 communicated with the strip-shaped groove 211, the two through holes 221 and the strip-shaped groove 211 together form a slider flow channel, and the two through holes 221 form an inlet and an outlet of the slider flow channel. One end of the chip flow channel is provided with a connecting hole on the surface of the chip body 10, and the relative sliding between the slider 20 and the chip body 10 can make the inlet and the outlet of the slider flow channel respectively butt-joint with the connecting holes of the two chip flow channels, so that a flow path is formed between the two chip flow channels. The upper and lower layers of the slider 20 can be connected by bonding methods such as hot pressing, gluing, ultrasonic welding, laser welding and the like.

The sliding block 20 is divided into an upper layer and a lower layer, and the bottom of the upper layer is provided with a groove and the lower layer is provided with a through hole, so that the sliding block flow channel on the sliding block 20 is more convenient and easier to process, and the processing precision of the sliding block flow channel is improved. The chip flow channel is communicated with the slide block flow channel through the end opening hole, so that the problem of fluid residue is further avoided, meanwhile, the independence of the flow channel is guaranteed, the occupied area of each flow channel communication port on the surface of the chip is reduced, and the integration level and the designability of the surface of the chip are improved.

In some embodiments, as shown in fig. 1, 10 and 11, the microfluidic chip further includes a pressing structure 30, the pressing structure 30 is fixedly connected to the chip body 10, and the pressing structure 30 presses the slider 20 on the surface of the chip body 10, so that the slider 20 is in close contact with the surface of the chip body when sliding. The sliding block 20 is pressed on the surface of the chip body 10 through the pressing structure 30, so that the sliding block 20 is in close contact with the chip 10, a sealing effect is achieved, and the reliability and the tightness of butt joint of a chip flow channel and a sliding block flow channel are improved.

In some embodiments, as shown in fig. 1, 10 and 11, the pressing structure 30 includes a first guide portion for defining a sliding direction of the slider 20 and/or a first stopper portion for defining a sliding distance of the slider 20. Through setting up first guide part for slider 20 can be followed the straight line and slided, makes the slider runner intercommunication and break off the chip runner more controllable and reliable. The first stopping portion is arranged to limit the sliding distance of the slider 20, so that the slider 20 and the chip body 10 can be accurately positioned or the idle stroke of the slider 20 can be reduced when communicating different flow channels and other specific positions, and the controllability of the operation of the slider 20 is further improved.

In some embodiments, as shown in fig. 1, 10 and 11, the compression structure includes a top plate, and the slider 20 is slidably disposed between the top plate and the chip body 10. The top plate comprises an opening or a sliding slot which is in sliding fit with the top of the slider 20, the inner wall of the opening or sliding slot forming a first guide and/or a first stop. As shown in fig. 1, 10 and 11, in some embodiments, the first guide portion includes a strip-shaped opening at the top of the pressing structure 30, and the upper portion of the slider 20 further includes a protrusion extending from the opening. The long-side inner wall of the opening defines a slider 20 that slides linearly on the surface of the chip body 10. And the inner wall of the short side of the opening forms a first stopper that limits the sliding distance of the slider 20. The middle part of the sliding block 20 is protruded, and the middle part of the pressing structure 30 is hollowed out, so that the protruded part of the sliding block 20 can be just protruded. Thus, when the pressing structure 30 presses the slider, the driving device can conveniently apply a force on the convex portion of the slider 20, thereby driving the slider to slide.

In some embodiments, a friction reducing structure is disposed between the pressing structure 30 and the sliding block 20 for reducing the friction between the pressing structure 30 and the sliding block 20.

In some embodiments, the portion of the compression structure 30 in contact with the slider 20 is provided with an arc-like projection or a layer of self-lubricating material. As shown in fig. 10, an arcuate projection 31a may be provided on a surface of the first guide portion of the hold-down structure 30 that contacts the slider 20. An arc-shaped protrusion 31b may be further provided on the surface of the pressing structure 30 for pressing the slider 20. By arranging the arc-shaped protrusion, the sliding contact area between the compressing structure 30 and the sliding block 20 can be reduced, and the sliding friction force between the sliding block 20 and the compressing structure 30 can be reduced while the guiding or compressing effect on the sliding block 20 is ensured. In a similar way, a self-lubricating material layer can be arranged at the position, the self-lubricating material layer has a self-lubricating function, and the friction force between the sliding block 20 and the pressing structure 30 can also be reduced. The self-lubricating material layer can be made of polytetrafluoroethylene and/or fluorinated ethylene propylene and the like.

In some embodiments, the anti-friction structure further comprises rolling bodies disposed between the compression structure 30 and the slider 20. The rolling bodies may be provided to reduce friction when the slider 20 slides relative to the compression structure 30 by changing sliding friction into rolling friction. The rolling bodies can be arranged in a chamber on the pressure structure 30 or the slide 20 and then directly in the chamber. As shown in fig. 11, a rolling element structure 60 may be disposed between the pressing structure 30 and the slider 20, where the rolling element structure 60 includes a holder and a rolling element located on the holder and capable of rotating relative to the holder, and the pressing structure 30 presses the slider 20 against the surface of the chip body 10 through the rolling element structure. The pressing structure 30 presses the slider 2 on the chip body 10 by providing the rolling body structure 60, the friction between the slider 20 and the pressing structure 30 when sliding is changed from sliding friction to rolling friction, and the friction between the slider 20 and the pressing structure 30 when sliding is further reduced.

In some embodiments, the microfluidic chip further comprises a second guide and/or a second stopper. The second guide portion is provided on the chip body 20, and the second guide portion defines a sliding direction of the slider 20. The second stopping portion is disposed on the chip body 20, and the second stopping portion defines a sliding distance between the slider 20 and the chip body 10. The provision of the second guide portion and the second stopper further improves the guiding ability of the slider 20 and the handleability of the slider 20. When the second guide portion and the second stopping portion are matched with the first guide portion and the first stopping portion, the sliding block 20 is more stable and reliable through guiding and stopping the upper end and the lower end of the sliding block 20.

In some embodiments, as shown in fig. 1 and 10, the second guide portion includes two pieces of block 42 in the linear sliding direction of the slider 20, the second stopper portion includes two pieces of block 41 perpendicular to the linear sliding direction of the slider 20, and the second guide portion and the second stopper portion form a receiving groove for receiving the bottom of the slider 20. The second guide part and the second stop part are arranged to be of a groove-shaped strip-shaped block accommodating structure, the structure is simple and reliable, and meanwhile, the two blocks 42 and the two blocks 41 can play a peripheral protection role on the micro-fluidic chip, so that foreign matters can be prevented from sliding into the bottom of the slide block 20, and influences on a slide block flow channel and a chip flow channel by the foreign matters can be prevented.

In some embodiments, the material of the slider 20 is plastic or rubber. The plastic or the rubber does not react with the biological reagent, can resist certain acidity and alkalinity, has better lubricity and sealing property, can be compressed on the surface of the chip body, realizes the sealing of the chip flow channel in the chip body, and can slide on the surface of the chip body under the driving of external force more smoothly. Further, the material of the slider 20 is preferably polytetrafluoroethylene, or soluble polytetrafluoroethylene capable of injection molding, or fluorinated ethylene propylene.

In some embodiments, the compressing structure 30 may be a plastic or metal material, and the plastic material commonly used in the microfluidic chip is, for example, one of polycarbonate, polymethyl methacrylate, polypropylene, polystyrene, polyethylene terephthalate, or cyclic olefin copolymer, which is non-reactive with biological reagents and is resistant to a certain acid and alkali. The microfluidic chip of the embodiment has better rigidity, and the contact surface of the microfluidic chip and the sliding block is smooth.

In some embodiments, the chip body 10 includes a multilayer structure. For example, in the embodiment shown in fig. 1, the chip body 10 includes a three-layer structure including a top layer plate 11, a middle layer plate 12, and a bottom layer plate 13. The top plate 11 is provided with a chip flow passage connecting hole, the middle plate is provided with a long hole, and after the top plate, the middle plate and the bottom plate are combined into a whole, the long hole is connected with the hole in the top plate to form a chip flow passage. The three-layer structure can be formed by bonding in the modes of gluing, hot pressing, ultrasonic welding, laser welding and the like. In some embodiments, as shown in fig. 10 and 11, the chip body 10 may further include a two-layer structure including a top layer plate 11 'and a bottom layer plate 12'. The top plate 11 comprises a chip flow passage connecting hole, the middle plate is provided with a long groove, and after the top plate 11 'and the bottom plate 12' are combined into a whole, the long groove is connected with an opening on the top plate to form a chip flow passage.

In some embodiments, the microfluidic chip includes a reinforcing structure disposed on a side of the chip body 10 away from the slider 20, and the reinforcing structure may be a reinforcing plate 70 as shown in fig. 11, and the reinforcing structure is used for reducing bending deformation of the chip body 10 toward the side away from the slider 20. After the compressing structure 30 compresses the slider 20 on the chip body 1, the middle portion of the chip body 10 may bend and deform downward, and the reinforcing structure is disposed at the bottom of the chip body 10, which helps to reduce the deformation and bending of the chip body 10, so that the microfluidic chip is more stable and reliable. Preferably, the material stiffness of the reinforcing structure is greater than the material stiffness of the chip body 10.

In some embodiments, the microfluidic chip may further include a plurality of sliders 20, and the plurality of sliders 20 may collectively or at least partially independently perform operations of connecting and disconnecting the chip channels.

Finally, it should be noted that: the above examples are only intended to illustrate the technical solution of the present invention and not to limit it; although the present invention has been described in detail with reference to preferred embodiments, those skilled in the art will understand that: modifications to the specific embodiments of the invention or equivalent substitutions for parts of the technical features may be made; without departing from the spirit of the present invention, it is intended to cover all aspects of the invention as defined by the appended claims.

Claims

1. A microfluidic chip, comprising:

the chip comprises a chip body (10), wherein more than two chip flow channels which are not communicated with each other are arranged in the chip body (10); and

the sliding block (20), the sliding block (20) and the chip body (10) are arranged on the chip body (10) in a relatively sliding mode, and a sliding block flow channel is arranged in the sliding block (20); the sliding of the sliding block (20) relative to the chip body (10) enables the sliding block flow channel to be communicated with different chip flow channels so as to form a flow path between the different chip flow channels.

2. The microfluidic chip according to claim 1, wherein the slider (20) slides relative to the chip body (10) to connect one slider channel with two chip channels to form a flow path between the two chip channels.

3. The microfluidic chip of claim 1, wherein the flow path is a non-branching line type flow path.

4. The microfluidic chip according to claim 1, wherein the slider (20) comprises a plurality of slider channels, and during the relative sliding of the slider (20) and the chip body (10), at most only one slider channel is communicated with at least two chip channels, and the chip channels communicated with different slider channels are not identical.

5. The microfluidic chip of claim 1,

the microfluidic chip further comprises a chip covering layer (50) arranged between the sliding block (20) and the chip body (10) and positioned on the surface of the chip body (10), through holes corresponding to the open holes of the chip flow channels positioned on the surface of the chip body (10) are formed in the chip covering layer (50), and the chip covering layer (50) is used for lubricating the sliding block (20) and/or sealing gaps between the sliding block flow channels and the chip flow channels; and/or

The micro-fluidic chip further comprises a sliding block covering layer which is arranged between the sliding block (20) and the chip body (10) and is positioned on the surface of the sliding block (20), through holes corresponding to the open holes of the sliding block flow channels positioned on the surface of the sliding block (20) are formed in the sliding block covering layer, and the sliding block covering layer is used for lubricating the sliding block (20) and/or sealing gaps between the sliding block flow channels and the chip flow channels.

6. The microfluidic chip according to claim 5, wherein the material of the chip cover layer (50) or the slider cover layer is polytetrafluoroethylene and/or fluorinated ethylene propylene.

7. The microfluidic chip according to claim 1, wherein the surface of the slider (20) is provided with a positioning portion corresponding to the position of the slider flow channel.

8. The microfluidic chip of claim 7, wherein the positioning portion comprises a protrusion corresponding to the slider flow channel one to one.

9. The microfluidic chip according to claim 1, wherein the slider (20) comprises an upper slider layer (21) and a lower slider layer (22) connected to the bottom of the upper slider layer (21), the bottom of the upper slider layer (21) is provided with a strip-shaped groove (211), the lower slider layer (22) is provided with two through holes (221) communicated with the strip-shaped groove, the two through holes (221) and the strip-shaped groove (211) together form the slider flow channel, and the two through holes (221) form an inlet and an outlet of the slider flow channel.

10. The microfluidic chip according to claim 1, further comprising a pressing structure (30), wherein the pressing structure (30) is fixedly connected to the chip body (10), and the pressing structure (30) presses the slider (20) against the surface of the chip body (10).

11. The microfluidic chip according to claim 10, wherein an anti-friction structure is provided between the compression structure (30) and the slider (20) for reducing friction between the compression structure (30) and the slider (20).

12. Microfluidic chip according to claim 11, wherein the friction reducing structure comprises an arc-like protrusion or a layer of self-lubricating material provided on the hold-down structure or the slider (20).

13. The microfluidic chip according to claim 11, wherein the anti-friction structure comprises rolling elements disposed between the compression structure (30) and the slider (20).

14. The microfluidic chip according to claim 13, wherein the anti-friction structure comprises a cage disposed between the hold-down structure (30) or the slider (20), the rolling element being located on the cage and rotatable relative to the cage.

15. The microfluidic chip according to claim 10, wherein the packing structure (30) comprises:

a first guide portion for defining a sliding direction of the slider (20); and/or

A first stop for defining a sliding distance of the slider (20).

16. The microfluidic chip according to claim 15, wherein the compressing structure comprises a top plate, the slider (20) is slidably disposed between the top plate and the chip body (10), the top plate comprises an opening or a sliding groove slidably engaged with a top of the slider (20), and an inner wall of the opening or the sliding groove forms the first guiding portion and/or the first stopping portion.

17. The microfluidic chip according to any of claims 1 to 16, wherein the microfluidic chip comprises a reinforcing structure disposed on a side of the chip body (10) away from the slider (20), the reinforcing structure configured to reduce bending deformation of the chip body (10) to the side away from the slider (20).

18. The microfluidic chip according to any of claims 1 to 16, wherein the microfluidic chip further comprises:

a second guide portion provided on the chip body (10), the second guide portion defining a sliding direction of the slider (20); and/or the presence of a gas in the gas,

the second stopping part is arranged on the chip body (10) and limits the relative sliding distance between the sliding block (20) and the chip body (10).

19. The microfluidic chip according to claim 18, wherein the second guide portion comprises two first bars (42) spaced in parallel, and the second stop portion comprises two second bars (41) spaced in parallel and perpendicular to the two first bars (42), and the two first bars (42) and the two second bars (41) form a receiving space for receiving a bottom of the slider (20).

20. The microfluidic chip according to any of claims 1 to 16, wherein the material of the slider (20) is plastic or rubber.