CN110850090A - Test strip for detecting bifenthrin and application thereof - Google Patents
Test strip for detecting bifenthrin and application thereof Download PDFInfo
- Publication number
- CN110850090A CN110850090A CN201911020025.7A CN201911020025A CN110850090A CN 110850090 A CN110850090 A CN 110850090A CN 201911020025 A CN201911020025 A CN 201911020025A CN 110850090 A CN110850090 A CN 110850090A
- Authority
- CN
- China
- Prior art keywords
- bifenthrin
- test strip
- pad
- hapten
- conjugate
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Pending
Links
Images
Classifications
-
- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
- G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
- G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
- G01N33/53—Immunoassay; Biospecific binding assay; Materials therefor
- G01N33/577—Immunoassay; Biospecific binding assay; Materials therefor involving monoclonal antibodies binding reaction mechanisms characterised by the use of monoclonal antibodies; monoclonal antibodies per se are classified with their corresponding antigens
-
- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
- G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
- G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
- G01N33/53—Immunoassay; Biospecific binding assay; Materials therefor
- G01N33/5308—Immunoassay; Biospecific binding assay; Materials therefor for analytes not provided for elsewhere, e.g. nucleic acids, uric acid, worms, mites
-
- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
- G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
- G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
- G01N33/53—Immunoassay; Biospecific binding assay; Materials therefor
- G01N33/558—Immunoassay; Biospecific binding assay; Materials therefor using diffusion or migration of antigen or antibody
Abstract
The invention discloses a test strip for detecting bifenthrin and application thereof. The test strip comprises a sample absorption pad (1), a conjugate release pad (2), a reaction membrane (3), a water absorption pad (4) and a bottom plate (7), wherein the reaction membrane is provided with a detection line (5) coated with a bifenthrin hapten-carrier protein conjugate and a quality control line (6) coated with a goat anti-mouse anti-antibody, and the conjugate release pad (2) is sprayed with a bifenthrin monoclonal antibody-colloidal gold marker. The invention also provides a method for detecting bifenthrin residues in masonry walls such as soil, door and window holes and the like by applying the bifenthrin test strip. The test strip provided by the invention has the characteristics of simple operation, high sensitivity, high detection speed, low cost and the like, and is suitable for screening and on-site monitoring of a large number of samples.
Description
Technical Field
The invention relates to a test strip for detecting bifenthrin and application thereof, in particular to a colloidal gold test strip for detecting bifenthrin, which is particularly suitable for quickly, qualitatively and quantitatively detecting bifenthrin residues in masonry walls such as soil, door and window holes and the like.
Background
Bifenthrin, also known as tianwangxing, chongxiaoling and Bifenning, is a high-efficiency synthetic pyrethrin insecticidal acaricide, and has the functions of contact poisoning and stomach poisoning and no systemic and fumigation action. Broad spectrum and high effect on preventing mite. The pesticide has quick action, does not move in soil, belongs to high-efficiency low-toxicity pesticide, and can still cause poisoning of people and livestock and seriously harm human health.
Because different proportions have different functions, the sanitary insecticide is a chemical product for preventing and controlling pests, is mainly applied to living environment of human living and is directly related to the health and life safety of people. In southern areas, the pesticide is often used for controlling termites, and when the content of bifenthrin is lower than 15ppm, the pesticide does not reach the standard and cannot play a role in pesticide barrier; when the content of bifenthrin is higher than 87ppm, the concentration exceeds the standard, and soil pollution is caused. Therefore, the method has practical significance for the detection method of the residual amount of the bifenthrin, and the detection range can detect 15ppm/87 ppm. Especially, the establishment of a rapid detection method is beneficial to improving supervision means and supervision level.
Disclosure of Invention
The invention aims to provide a bifenthrin residue detection test strip which is high in sensitivity, simple to operate, low in cost and short in detection time.
The test strip for detecting bifenthrin residues comprises a sample absorption pad (1), a conjugate release pad (2), a reaction membrane (3), a water absorption pad (4) and a bottom plate (7); the reaction membrane is provided with a detection line (5) coated with a bifenthrin hapten-carrier protein conjugate and a quality control line (6) coated with a goat anti-mouse anti-antibody, and the conjugate release pad (2) is sprayed with a bifenthrin monoclonal antibody-colloidal gold marker.
The bifenthrin hapten-carrier protein conjugate is obtained by coupling bifenthrin hapten and carrier protein, wherein the carrier protein is bovine serum albumin, ovalbumin, hemocyanin, thyroid protein and human serum albumin.
The bifenthrin monoclonal antibody is prepared by taking a bifenthrin hapten-carrier protein conjugate as an immunogen and is obtained by secreting a bifenthrin monoclonal antibody hybridoma cell strain; the goat anti-mouse anti-antibody is obtained by immunizing a goat with a murine antibody.
The sample absorption pad (1), the conjugate release pad (2), the reaction film (3) and the water absorption pad (4) are sequentially adhered to the bottom plate (7), and the conjugate release pads 1/3-1/2 are covered under the sample absorption pad.
The bottom plate can be a PVC bottom plate or other hard non-absorbent materials; the sample absorption pad can be suction filter paper or oil filter paper; the conjugate release pad may be a glass wool or polyester material; the water absorption pad is absorbent paper; the reaction membrane can be a nitrocellulose membrane or a cellulose acetate membrane.
Another object of the present invention is to provide a method for preparing the test strip, which comprises the steps of:
1) preparing a conjugate release pad sprayed with a bifenthrin monoclonal antibody-colloidal gold marker;
2) preparing a reaction membrane with a detection line coated with bifenthrin hapten-carrier protein conjugate and a quality control line coated with goat anti-mouse anti-antibody;
3) assembling the conjugate release pad and the reaction membrane prepared in the steps 1) and 2) with a sample absorption pad, a water absorption pad and a base plate to form the test strip.
Specifically, the steps include:
1) hapten preparation: carrying out chemical reaction to obtain bifenthrin hapten;
2) coupling bifenthrin hapten with carrier protein to obtain a bifenthrin hapten-carrier protein conjugate;
3) immunizing a mouse by using the bifenthrin hapten-carrier protein conjugate, and fusing and screening spleen cells and myeloma cells of the mouse to obtain a bifenthrin monoclonal hybridoma cell strain;
4) extracting mouse IgG to immunize healthy goats to obtain goat anti-mouse anti-antibodies;
5) preparing colloidal gold by reacting trisodium citrate with chloroauric acid;
6) adding the bifenthrin monoclonal antibody prepared in the step 3) into the colloidal gold prepared in the step 5) to obtain a bifenthrin monoclonal antibody-colloidal gold marker;
7) spraying a bifenthrin monoclonal antibody-colloidal gold marker on a conjugate release pad, drying at 37 ℃ for 1h, taking out, and storing in a dry environment for later use;
8) coating the bifenthrin hapten-carrier protein conjugate on a reaction membrane to form a detection line, and coating a goat anti-mouse anti-antibody on the reaction membrane to form a quality control line;
9) soaking the sample absorption pad in 0.5% bovine serum albumin (volume fraction), pH7.2, 0.1mol/L phosphate buffer solution for 2h, and drying at 37 deg.C for 2 h;
10) and sequentially adhering a sample absorption pad, a conjugate release pad, a reaction film and a water absorption pad on the bottom plate, covering the conjugate release pad with the sample absorption pad, finally cutting into small strips with the width of 3mm, adding a plastic box, carrying out vacuum packaging, and storing for 12 months at the temperature of 4-30 ℃.
The invention also aims to provide a method for detecting bifenthrin residues in vegetables and crops by using the test strip, which comprises the following steps:
(1) pretreating a sample;
(2) detecting by using a test strip;
(3) and analyzing the detection result.
The bifenthrin rapid detection test strip adopts a highly specific antibody-antigen reaction and competitive inhibition immunochromatography analysis technology, a bifenthrin monoclonal antibody-colloidal gold marker is fixed on a conjugate release pad, and bifenthrin in a sample is combined with the bifenthrin monoclonal antibody-colloidal gold marker on the conjugate release pad in the flowing process to form a drug-antibody-colloidal gold marker. The drug in the sample and the bifenthrin hapten-carrier protein conjugate on the reaction film detection line compete to combine with the bifenthrin monoclonal antibody-colloidal gold marker, and whether the bifenthrin residue exists in the sample liquid to be detected is judged according to the existence of the red strip of the detection line or the color depth.
During detection, a sample is treated and then dropped into a test strip hole, when the concentration of bifenthrin in the sample is lower than the detection limit or zero, the monoclonal antibody-colloidal gold marker is combined with a bifenthrin hapten-carrier protein conjugate fixed on a reaction membrane in the chromatography process, and a red strip appears in a detection line (T) and a quality control line (C); if the concentration of bifenthrin in the sample is equal to or above the limit of detection, the monoclonal antibody-colloidal gold label will bind to all of the bifenthrin and no red band will appear at the T-line because the competition reaction will not bind to the bifenthrin hapten-carrier protein conjugate.
The test strip has the advantages of high sensitivity, strong specificity, low cost, simple operation, short detection time, suitability for various units, simple storage and long quality guarantee period. The method for detecting bifenthrin residues by using the test strip is simple, convenient, rapid, visual, accurate, wide in application range, low in cost and easy to popularize and use.
Drawings
FIG. 1 is a diagram of bifenthrin hapten synthesis.
FIG. 2 is a schematic cross-sectional view of a test strip.
Detailed Description
The invention is further illustrated below with reference to specific examples. It should be understood that these examples are for illustrative purposes only and are not intended to limit the scope of the present invention.
Example 1 preparation of bifenthrin test strip
The preparation method of the test strip mainly comprises the following steps:
1) preparing a conjugate release pad sprayed with a bifenthrin monoclonal antibody-colloidal gold marker;
2) preparing a reaction membrane with a detection line coated with bifenthrin hapten-carrier protein conjugate and a quality control line coated with goat anti-mouse anti-antibody;
3) assembling the conjugate release pad and the reaction membrane prepared in the steps 1) and 2) with a sample absorption pad, a water absorption pad and a PVC base plate to form the test strip.
The following steps are detailed:
1. preparation of bifenthrin hapten
Taking 0.322g of the compound a, adding 30ml of methanol for dissolving, clarifying to obtain solution A, taking 0.155g of the compound b, adding 2ml of water for dissolving, adding the compound b into the solution A, stirring for 2 hours at room temperature, stopping reaction, carrying out rotary evaporation, removing the methanol, adding 60ml of water, extracting by 80ml of ethyl acetate, separating out an aqueous phase, concentrating and evaporating an organic phase to dryness, loading on a silica gel column, eluting and separating by petroleum ether/ethyl acetate (v/v, 5/1) to obtain 0.38g of a bifenthrin hapten product compound c, wherein the yield is 89.83%.
2. Preparation of immunogens
Taking 16mg of bifenthrin hapten, adding 1ml of DMF for dissolving, clarifying, adding 14.2mg of EDC, adding 9.87mg of NHS, and reacting at room temperature for 2h to obtain a hapten activating solution A; taking Bovine Serum Albumin (BSA), adding 0.05M PB buffer solution for dissolving to obtain solution B, dripping A solution into the solution B, reacting for 4 hours at room temperature, dialyzing and purifying for 3 days by using 0.02M PBS, changing the solution for 3 times every day, centrifuging and packaging to obtain a bifenthrin-BSA conjugate which is an immunogen, and storing at-20 ℃ for later use.
3. Preparation of coating antigen
Taking 18mg of bifenthrin hapten, adding 1ml of DMF for dissolving, clarifying, adding 16mg of EDC, adding 14mg of NHS, and reacting at room temperature for 2h to obtain a hapten activation solution A; dissolving egg serum albumin (OVA) in 0.05M PB buffer solution to obtain solution B, dripping A solution into solution B, reacting at room temperature for 4h, dialyzing and purifying with 0.02M PBS for 3 days, changing solution 3 times per day, centrifuging, and packaging to obtain bifenthrin-OVA conjugate, which is coating antigen, and storing at-20 deg.C.
4. Preparation of bifenthrin monoclonal antibody
(1) Animal immunization
Injecting the immunogen obtained in the step 2 into Balb/c mice at an immune dose of 150 mug/mouse to generate antiserum.
(2) Cell fusion and cloning
Taking immune Balb/c mouse spleen cells, fusing the immune Balb/c mouse spleen cells with SP2/0 myeloma cells according to the proportion of 8: 1 (quantity ratio), measuring cell supernatant by adopting an indirect competitive ELISA method, and screening positive holes. Cloning the positive hole by using a limiting dilution method until obtaining the hybridoma cell strain which stably secretes the monoclonal antibody.
(3) Cell cryopreservation and recovery
Making hybridoma cell into 1 × 10 with frozen stock solution6Cell suspension per ml, preserved for long periods in liquid nitrogen. Taking out the frozen tube during recovery, immediately putting the tube into a water bath at 37 ℃ for fast melting, centrifuging to remove frozen liquid, and transferring the tube into a culture bottle for culture.
(4) Preparation and purification of monoclonal antibodies
An incremental culture method: placing the hybridoma cell in cell culture medium, culturing at 37 deg.C, purifying the obtained culture solution by octanoic acid-saturated ammonium sulfate method to obtain monoclonal antibody, and storing at-20 deg.C.
The cell culture medium is prepared by adding calf serum and sodium bicarbonate into RPMI1640 culture medium to make the final concentration of calf serum in the cell culture medium 20% (mass fraction) and the final concentration of sodium bicarbonate in the cell culture medium 0.2% (mass fraction); the pH of the cell culture medium was 7.4.
5. Preparation of goat anti-mouse anti-antibody
The sheep is taken as an immune animal, and the pathogen-free sheep is immunized by taking the murine antibody as an immunogen to obtain the goat anti-mouse antibody.
6. Preparation of bifenthrin monoclonal antibody-colloidal gold marker
(1) Preparation of colloidal gold
Diluting 1% chloroauric acid to 0.01% (mass fraction) with double distilled deionized water, placing 100ml into a conical flask, heating to boil with a constant temperature electromagnetic stirrer, adding 2.5ml 1% trisodium citrate under continuous stirring at high temperature, stirring at constant speed, heating until the solution is bright red, cooling to room temperature, recovering to original volume with deionized water, and storing at 4 deg.C. The prepared colloidal gold has pure appearance, transparency and no precipitate or floating material.
(2) Preparation of bifenthrin monoclonal antibody-colloidal gold marker
Under magnetic stirring, adjusting the pH value of the colloidal gold to 7.0 by using 0.2mol/L potassium carbonate solution, adding bifenthrin monoclonal antibody into the colloidal gold solution according to the standard of adding 20-50 mu g of bifenthrin monoclonal antibody into each milliliter of the colloidal gold solution, continuously stirring and uniformly mixing for 30min, adding 10% BSA (bovine serum albumin) to ensure that the final concentration of the BSA in the colloidal gold solution is 1% (volume fraction), and standing for 10 min. Centrifuging at 12000r/min at 4 deg.C for 40min, discarding supernatant, washing the precipitate with redissolving buffer twice, resuspending the precipitate with redissolving buffer whose volume is 1/10 of the original volume of colloidal gold, and standing at 4 deg.C for use.
Redissolving buffer solution: 0.02mol/L phosphate buffer solution containing casein 0.02-0.1% (mass fraction), tween-800.05-0.2% (mass fraction) and pH7.2.
7. Preparation of conjugate Release pad
The conjugate release pad was soaked in 0.5mol/L phosphate buffer containing bovine serum albumin (concentration of bovine serum albumin in buffer is 0.5%), pH7.2, and the solution was soaked for 1h, and then baked at 37 ℃ for 3 h. And uniformly spraying the prepared bifenthrin monoclonal antibody-colloidal gold marker on a conjugate release pad by using an Isoflow film spraying instrument, spraying 0.01ml of bifenthrin monoclonal antibody-colloidal gold marker on each 1cm of conjugate release pad, placing the mixture in an environment at 37 ℃ (humidity is less than 20%) for 60min, taking out the mixture, and placing the mixture in a dry environment (humidity is less than 20%) for storage for later use.
8. Preparation of the reaction film
And coating the bifenthrin hapten-ovalbumin conjugate on a reaction membrane to form a detection line, and coating the goat anti-mouse anti-antibody on the reaction membrane to form a quality control line.
Coating process: diluting the bifenthrin hapten-ovalbumin conjugate to 10mg/ml by using a phosphate buffer solution, and coating the conjugate on a detection line (T line) on a nitrocellulose membrane by using an Isoflow point membrane instrument, wherein the coating amount is 0.8 mu l/cm; the goat anti-mouse anti-antibody was diluted to 200. mu.g/ml with 0.01mol/L, pH7.4 phosphate buffer, and coated on a quality control line (C line) on a nitrocellulose membrane in an amount of 1.0. mu.l/cm using an Isoflow dot membrane apparatus. And (3) drying the coated reaction membrane for 2 hours at 37 ℃ for later use.
9. Preparation of sample absorbent pad
The sample absorption pad is soaked in 0.5% bovine serum albumin (volume fraction), pH7.2, 0.1mol/L phosphate buffer solution for 2h, and then baked at 37 ℃ for 2h for standby.
10. Assembly of test strips
Sequentially adhering a sample absorption pad, a conjugate release pad, a reaction membrane and a water absorption pad on a PVC bottom plate; the binder release pad is covered by the sample absorption pad from the 1/3 area at the starting end, the tail end of the binder release pad is connected with the starting end of the reaction film, the tail end of the reaction film is connected with the starting end of the water absorption pad, the starting end of the sample absorption pad is aligned with the starting end of the PVC soleplate, and the tail end of the water absorption pad is aligned with the tail end of the PVC soleplate; the reaction membrane is provided with a detection line and a quality control line, and the detection line (T line) and the quality control line (C line) are strip-shaped strips which are vertical to the long phase of the test strip; the detection line is located on the side near the end of the conjugate release pad; the quality control line is positioned on the side away from the end of the conjugate release pad; the test paper strip is cut into small strips with the width of 3mm by a machine, and the small strips are arranged in a special plastic card and can be stored for 12 months at the temperature of 4-30 ℃.
Example 2 detection of bifenthrin residue in samples
1. Detection with test strip
And (3) sucking the sample solution to be detected by using a suction pipe, vertically and dropwise adding the sample solution to be detected into the sample adding hole, starting timing when the liquid flows, reacting for 5-10 min, and judging the result.
2. Analyzing the results of the detection
The results were read by a colloidal gold analyzer (abbreviated as "analyzer"):
negative (-): indicating that the concentration of the substance to be detected in the sample is lower than the detection limit;
positive (+): indicating that the concentration of the substance to be detected in the sample is equal to or higher than the detection limit;
and (4) invalidation: indicating that retesting is required.
Example 3 sample testing example
1. Limit of detection test
Taking blank soil and door and window hole samples, respectively adding bifenthrin into the blank soil and the door and window hole samples until the final concentration is 7.5, 15 and 30mg/kg, taking test strips for detection, and repeatedly measuring each sample for three times.
When the test paper is used for detecting soil and door and window hole samples, the analyzer shows that the bifenthrin is negative when the addition concentration of the bifenthrin is 7.5 mg/kg; when the bifenthrin is added at a concentration of 15mg/kg and 30mg/kg, the analyzer shows positive.
2. Test for false positive and false negative rates
Taking 20 parts of soil with the known bifenthrin content of 15mg/kg and 20 parts of positive samples of doors and windows and 20 parts of soil with the content of less than 15mg/kg and 20 parts of negative samples of doors and windows, detecting by using three batches of test strips, judging by using a qualitative method and a quantitative method, and calculating the negative and positive rates of the samples. The results are shown in tables 1 and 2.
TABLE 1 soil test sample results
TABLE 2 door and window hole test sample results
The results show that: when 3 test strips produced in batches are used for detecting positive soil and door and window hole samples, the results of the qualitative method and the quantitative method are consistent, namely the results are all positive, the coincidence rate of the positive samples is 100 percent, and the false negative rate is 0; when 20 negative soil and door and window hole samples are detected, the results of the qualitative method and the quantitative method are consistent, namely, the negative soil and the door and window hole samples are all negative, the negative coincidence rate is 100%, and the false positive rate is 0. The test strip for detecting bifenthrin can be used for rapidly detecting bifenthrin residues in soil and door and window holes.
3. Specificity test
The bifenthrin test paper is used for detecting 500mg/kg of cypermethrin, fenvalerate, deltamethrin and other medicines. The result shows that the quality control line and the detection line of the test strip are both colored and negative. The test paper has no cross reaction to 500mg/kg of cypermethrin, fenvalerate, deltamethrin and other medicaments.
Claims (8)
1. A test strip for detecting bifenthrin comprises a sample absorption pad (1), a conjugate release pad (2), a reaction membrane (3), a water absorption pad (4) and a bottom plate (7), and is characterized in that the reaction membrane is provided with a detection line (5) coated with a bifenthrin hapten-carrier protein conjugate and a quality control line (6) coated with a goat anti-mouse anti-antibody, the conjugate release pad (2) is sprayed with a bifenthrin monoclonal antibody-colloidal gold marker, and the preparation method of the bifenthrin hapten is as follows:
taking 0.322g of the compound a, adding 30ml of methanol for dissolving, clarifying to obtain solution A, taking 0.155g of the compound b, adding 2ml of water for dissolving, adding the compound b into the solution A, stirring for 2 hours at room temperature, stopping reaction, carrying out rotary evaporation, removing the methanol, adding 60ml of water, extracting by 80ml of ethyl acetate, separating out an aqueous phase, concentrating and evaporating an organic phase to dryness, loading on a silica gel column, eluting and separating by petroleum ether/ethyl acetate (v/v, 5/1) to obtain 0.38g of a bifenthrin hapten product compound c, wherein the yield is 89.83%.
2. The test strip of claim 1, wherein the sample absorbing pad (1), the conjugate releasing pad (2), the reaction membrane (3) and the absorbent pad (4) are sequentially adhered to the bottom plate (7).
3. The strip of any one of claims 1-2, wherein the conjugate release pad 1/3-1/2 is covered under a sample absorbing pad.
4. The test strip of claim 1, wherein the bifenthrin hapten-carrier protein conjugate is obtained by coupling bifenthrin hapten with a carrier protein, and the carrier protein is bovine serum albumin, ovalbumin, hemocyanin, thyroid protein, or human serum albumin.
5. The test strip of any one of claims 1 or 4, wherein the bifenthrin hapten is obtained by a chemical reaction and has a molecular structural formula:
6. the test strip of claim 1, wherein the bifenthrin monoclonal antibody is prepared by using a bifenthrin hapten-carrier protein conjugate as an immunogen, and the goat anti-mouse anti-antibody is obtained by immunizing a goat with a murine antibody.
7. A method of making the test strip of any one of claims 1-6, comprising the steps of:
1) preparing a conjugate release pad sprayed with a bifenthrin monoclonal antibody-colloidal gold marker;
2) preparing a reaction membrane with a detection line coated with bifenthrin hapten-carrier protein conjugate and a quality control line coated with goat anti-mouse anti-antibody;
3) assembling the conjugate release pad and the reaction membrane prepared in the steps 1) and 2) with a sample absorption pad, a water absorption pad and a base plate to form the test strip.
8. A method for detecting bifenthrin residues in masonry walls such as soil, door and window holes and the like comprises the following steps:
1) sample pretreatment;
2) performing a test using the test strip of any one of claims 1-6;
3) and analyzing the detection result.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201911020025.7A CN110850090A (en) | 2019-10-25 | 2019-10-25 | Test strip for detecting bifenthrin and application thereof |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201911020025.7A CN110850090A (en) | 2019-10-25 | 2019-10-25 | Test strip for detecting bifenthrin and application thereof |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| CN110850090A true CN110850090A (en) | 2020-02-28 |
Family
ID=69596889
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN201911020025.7A Pending CN110850090A (en) | 2019-10-25 | 2019-10-25 | Test strip for detecting bifenthrin and application thereof |
Country Status (1)
| Country | Link |
|---|---|
| CN (1) | CN110850090A (en) |
Cited By (5)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN111735952A (en) * | 2020-06-04 | 2020-10-02 | 北京勤邦生物技术有限公司 | Test strip and method for detecting hexachlorobenzene |
| CN111735951A (en) * | 2020-06-03 | 2020-10-02 | 北京勤邦生物技术有限公司 | Test strip for detecting fenpropathrin and application thereof |
| CN111856000A (en) * | 2020-06-04 | 2020-10-30 | 北京勤邦生物技术有限公司 | Test strip and method for detecting chlordane |
| CN111965359A (en) * | 2020-07-20 | 2020-11-20 | 北京勤邦生物技术有限公司 | Test strip and method for detecting chlorfenapyr |
| CN113030463A (en) * | 2021-02-04 | 2021-06-25 | 北京勤邦生物技术有限公司 | Test strip for detecting protein A and other impurities in vaccine and application thereof |
-
2019
- 2019-10-25 CN CN201911020025.7A patent/CN110850090A/en active Pending
Cited By (10)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN111735951A (en) * | 2020-06-03 | 2020-10-02 | 北京勤邦生物技术有限公司 | Test strip for detecting fenpropathrin and application thereof |
| CN111735951B (en) * | 2020-06-03 | 2022-11-18 | 北京勤邦生物技术有限公司 | Test strip for detecting fenpropathrin and application thereof |
| CN111735952A (en) * | 2020-06-04 | 2020-10-02 | 北京勤邦生物技术有限公司 | Test strip and method for detecting hexachlorobenzene |
| CN111856000A (en) * | 2020-06-04 | 2020-10-30 | 北京勤邦生物技术有限公司 | Test strip and method for detecting chlordane |
| CN111856000B (en) * | 2020-06-04 | 2023-07-07 | 北京勤邦科技股份有限公司 | Test strip and method for detecting chlordane |
| CN111735952B (en) * | 2020-06-04 | 2023-07-11 | 北京勤邦科技股份有限公司 | Test strip and method for detecting hexachlorobenzene |
| CN111965359A (en) * | 2020-07-20 | 2020-11-20 | 北京勤邦生物技术有限公司 | Test strip and method for detecting chlorfenapyr |
| CN111965359B (en) * | 2020-07-20 | 2023-06-16 | 北京勤邦科技股份有限公司 | Test strip and method for detecting chlorfenapyr |
| CN113030463A (en) * | 2021-02-04 | 2021-06-25 | 北京勤邦生物技术有限公司 | Test strip for detecting protein A and other impurities in vaccine and application thereof |
| CN113030463B (en) * | 2021-02-04 | 2023-08-11 | 北京邦腾生物科技有限公司 | Test strip for detecting impurities such as protein A in vaccine and application thereof |
Similar Documents
| Publication | Publication Date | Title |
|---|---|---|
| CN110850090A (en) | Test strip for detecting bifenthrin and application thereof | |
| CN109324182B (en) | Fluorescent microsphere immunochromatography test strip for detecting pendimethalin and preparation method and application thereof | |
| CN109239336B (en) | Test strip for detecting isoprocarb and application thereof | |
| CN110441518B (en) | Test strip and method for detecting gibberellin | |
| CN109061146B (en) | Test strip for detecting acetamiprid and preparation method and application thereof | |
| CN105675874A (en) | Colloidal gold test strip for detection of imidacloprid and application thereof | |
| CN111239399A (en) | Test strip and method for detecting profenofos | |
| CN107271665B (en) | Test strip for detecting salbutamol and application thereof | |
| CN106918705B (en) | Test paper for detecting fenpropathrin and application thereof | |
| CN109682961B (en) | Test strip for detecting cyphenothrin and application thereof | |
| CN112067811B (en) | Test strip for detecting alternaria alternate and application thereof | |
| CN112067814A (en) | Test strip and method for detecting difenoconazole | |
| CN108931640B (en) | Test strip for detecting organophosphorus pesticide and application thereof | |
| CN111735951B (en) | Test strip for detecting fenpropathrin and application thereof | |
| CN110551220A (en) | Preparation and application of DDT monoclonal antibody | |
| CN113156125B (en) | Test strip and method for detecting milbemycetin | |
| CN111751535B (en) | Test strip for detecting endosulfan and application thereof | |
| CN113030463B (en) | Test strip for detecting impurities such as protein A in vaccine and application thereof | |
| CN111965360B (en) | Test strip and method for detecting procymidone | |
| CN111896738B (en) | Test strip for detecting serpentine bacterial element and application thereof | |
| CN109061145B (en) | Test strip for detecting flumetralin, preparation method and application thereof | |
| CN112698026B (en) | Test strip for detecting clothianidin and application thereof | |
| CN111965359B (en) | Test strip and method for detecting chlorfenapyr | |
| CN111289752A (en) | Test strip and method for detecting dicofol | |
| CN111856000B (en) | Test strip and method for detecting chlordane |
Legal Events
| Date | Code | Title | Description |
|---|---|---|---|
| PB01 | Publication | ||
| PB01 | Publication | ||
| WD01 | Invention patent application deemed withdrawn after publication |
Application publication date: 20200228 |
|
| WD01 | Invention patent application deemed withdrawn after publication |