CN110840964A - Preparation method of lycium barbarum glycopeptide - Google Patents
Preparation method of lycium barbarum glycopeptide Download PDFInfo
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- A61K36/18—Magnoliophyta (angiosperms)
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- A61K36/81—Solanaceae (Potato family), e.g. tobacco, nightshade, tomato, belladonna, capsicum or jimsonweed
- A61K36/815—Lycium (desert-thorn)
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- A23L33/00—Modifying nutritive qualities of foods; Dietetic products; Preparation or treatment thereof
- A23L33/10—Modifying nutritive qualities of foods; Dietetic products; Preparation or treatment thereof using additives
- A23L33/105—Plant extracts, their artificial duplicates or their derivatives
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- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
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Abstract
The invention discloses a preparation method of lycium barbarum glycopeptide, relates to the technical field of lycium barbarum glycopeptide extraction, and comprises the following steps: s100, weighing and cleaning the raw materials of the dried Chinese wolfberry fruits; s200, leaching the cleaned dried Chinese wolfberry fruit raw material and collecting Chinese wolfberry fruit raw pulp; s300, removing the wolfberry seeds from the original wolfberry pulp by a double-channel beater, and collecting pulp A; s400, centrifuging the slurry A, and collecting filtrate B; s500, separating the filtrate B through an inorganic membrane, and collecting a filtrate C; s600, separating the filtrate C through an organic membrane, and collecting trapped fluid D; s700, single-effect concentration is carried out on the trapped fluid D, and a concentrated solution E is collected; s800, carrying out vacuum freeze drying on the concentrated solution E to obtain the lycium barbarum glycopeptide. The lycium barbarum glycopeptide prepared by the method has a complete active structure and stable physicochemical properties, and can be used as raw materials of foods, special dietary foods, special medical purpose foods, medicines and medicines.
Description
Technical Field
The invention relates to the technical field of lycium barbarum glycopeptide extraction, and particularly relates to a preparation method of lycium barbarum glycopeptide.
Background
The medlar is a mature fruit of medlar which belongs to medlar in solanaceae, is a dual-purpose food approved by the Ministry of health, is a traditional Chinese medicinal material and a tonic in China, and has various health care effects. A glycopeptide of fructus Lycii (also known as glycoprotein and glycoconjugate) is a glycoconjugate extracted from fructus Lycii, and is effective component of fructus Lycii for invigorating kidney, replenishing essence, improving immunity, and prolonging life. It is a water-soluble glycoprotein, and many studies show that the lycium barbarum glycopeptide has the effects of improving immunity, resisting aging, resisting tumors, eliminating free radicals, resisting fatigue, resisting radiation, protecting the liver, protecting and improving reproductive function and the like.
Most of the existing extraction processes of lycium barbarum glycopeptide are a traditional hot water extraction method or a hot water extraction method after organic solvent degreasing, an ethanol precipitation method and the like, and the methods have the disadvantages of large solvent consumption, low production safety coefficient, long time consumption and low extraction rate, so in order to improve the economic benefit of lycium barbarum and the extraction rate of effective components in lycium barbarum, technical personnel in the field are dedicated to developing a rapid and effective lycium barbarum glycopeptide extraction method, and the extraction method is widely applied to the technical field of lycium barbarum glycopeptide extraction.
Disclosure of Invention
In view of the above-mentioned drawbacks of the prior art, those skilled in the art have made efforts to develop a rapid and efficient method for extracting lycium barbarum glycopeptide, and make it widely applicable to the technical field of lycium barbarum glycopeptide extraction.
In order to realize the purpose, the invention provides a preparation method of lycium barbarum glycopeptide, which comprises the following steps:
s100, weighing and cleaning the raw materials of the dried Chinese wolfberry fruits;
s200, leaching the cleaned dried Chinese wolfberry fruit raw material and collecting Chinese wolfberry fruit raw pulp;
s300, removing the wolfberry seeds from the original wolfberry pulp by a double-channel beater, and collecting pulp A;
s400, centrifuging the slurry A, and collecting filtrate B;
s500, separating the filtrate B through an inorganic membrane, and collecting a filtrate C;
s600, separating the filtrate C through an organic membrane, and collecting trapped fluid D;
s700, single-effect concentration is carried out on the trapped fluid D, and a concentrated solution E is collected;
s800, carrying out vacuum freeze drying on the concentrated solution E to obtain the lycium barbarum glycopeptide.
Compared with the prior art, the preparation method of the lycium barbarum glycopeptide has the following technical advantages:
(1) the method adopts pure water for extraction, and does not add any organic solvent;
(2) the method has the advantages of short time consumption and high extraction efficiency by adopting ultrasonic stirring extraction;
(3) the method adopts a membrane filtration system to replace an alcohol precipitation method of the traditional process to prepare the lycium barbarum glycopeptide, reduces the production cost, not only prevents the components of the lycium barbarum glycopeptide from being influenced by denaturation caused by ethanol, but also removes water-soluble flavonoid glycoside, pigment, oligosaccharide and micromolecular polysaccharide, increases the content of the active macromolecule lycium barbarum glycopeptide in unit mass, and ensures the bioactivity of the lycium barbarum glycopeptide;
(4) the lycium barbarum glycopeptide prepared by the method has a complete active structure and stable physicochemical properties, and can be used as a raw material of food, food with special medical application and medicines.
The conception, the specific structure and the technical effects of the present invention will be further described with reference to the accompanying drawings to fully understand the objects, the features and the effects of the present invention.
Drawings
FIG. 1 is a flow chart of the method for preparing a glycopeptide of Lycium barbarum of the present invention.
Detailed Description
The technical contents of the preferred embodiments of the present invention will be more clearly and easily understood by referring to the drawings attached to the specification. The present invention may be embodied in many different forms of embodiments and the scope of the invention is not limited to the embodiments set forth herein.
Fig. 1 shows a flow chart of a method for preparing a glycopeptide of lycium barbarum according to a preferred embodiment of the present invention, which comprises the following steps:
s100, weighing and cleaning the raw materials of the dried Chinese wolfberry fruits;
s200, leaching the cleaned dried Chinese wolfberry fruit raw material and collecting Chinese wolfberry fruit raw pulp;
s300, removing the wolfberry seeds from the original wolfberry pulp by a double-channel beater, and collecting pulp A;
s400, centrifuging the slurry A, and collecting filtrate B;
s500, separating the filtrate B through an inorganic membrane, and collecting a filtrate C;
s600, separating the filtrate C through an organic membrane, and collecting trapped fluid D;
s700, single-effect concentration is carried out on the trapped fluid D, and a concentrated solution E is collected;
s800, carrying out vacuum freeze drying on the concentrated solution E to obtain the lycium barbarum glycopeptide.
On one hand, the preparation method of the lycium barbarum glycopeptide adopts pure water extraction, and no organic solvent is added;
on the other hand, the method provided by the invention has the advantages that the ultrasonic stirring extraction time is short, and the extraction efficiency is high;
secondly, the method adopts a membrane filtration system to replace the alcohol precipitation method of the traditional process to prepare the lycium barbarum glycopeptide, reduces the production cost, not only prevents the components of the lycium barbarum glycopeptide from being influenced by denaturation caused by ethanol, but also removes water-soluble flavonoid glycoside, pigment, oligosaccharide and micromolecular polysaccharide, increases the content of the active macromolecular lycium barbarum glycopeptide in unit mass, and ensures the bioactivity of the lycium barbarum glycopeptide;
finally, the lycium barbarum glycopeptide prepared by the method has a complete active structure and stable physicochemical properties, and can be used as a raw material of food, food with special medical application and medicines.
In a preferred embodiment, step S100 further includes: weighing the dried wolfberry fruit raw materials and pouring the dried wolfberry fruit raw materials into a lifting machine, conveying the dried wolfberry fruit raw materials to a spray cleaning machine through the lifting machine, and vibrating, spraying and cleaning the dried wolfberry fruit raw materials by using purified water.
In a preferred embodiment, step 200 further comprises:
s201, pumping cleaned dry Chinese wolfberry fruit raw materials into a primary sterile storage tank through a sanitary pump, adding purified water with the weight being 3-5 times that of the dry Chinese wolfberry fruit raw materials into the primary sterile storage tank, setting the temperature of the primary sterile storage tank at 30-80 ℃, stirring and soaking for 0.5-1h, and crushing the soaked dry Chinese wolfberry fruit raw materials through a crusher and a colloid mill to obtain primary Chinese wolfberry fruit raw pulp;
and S202, pumping the primary wolfberry original pulp into a secondary sterile storage tank by a sanitary pump, adding purified water with the weight of 3-5 of the dry wolfberry raw material weighed in the step S100 again, setting the temperature of the secondary sterile storage tank at 30-80 ℃, stirring at the speed of 50-100r/min and ultrasonic frequency of 20-30 kHz, extracting for 0.5-1h to obtain and collect the wolfberry original pulp.
In a preferred embodiment, step S400 further includes: separating coarse fructus Lycii pulp from the slurry A by horizontal screw centrifuge at 3000-4000f/f/min, separating fructus Lycii pulp from the filtrate B by butterfly centrifuge at 7000-8000r/min, and collecting filtrate B.
In a preferred embodiment, the inorganic membrane in step S500 is one of a metal membrane, an alloy membrane, a ceramic membrane, a molecular sieve composite membrane, a zeolite membrane, and a glass membrane, wherein in the inventive method of the present application, the inorganic membrane is a ceramic membrane, because the ceramic membrane has the following characteristics:
1) the ceramic membrane has narrow pore size distribution, so the separation precision is high, the pollution resistance is strong, and the cleaning and the recovery are easy;
2) the ceramic membrane has high mechanical strength, good thermal stability, temperature resistance and pressure resistance, and can conveniently introduce auxiliary processes such as back flushing, ultrasonic waves, surface turbulence reinforcement and the like;
3) the ceramic membrane has good chemical stability, good acid resistance and alkali resistance and long service life, and has considerable advantages in chemical industry, energy, food, bioengineering and pharmaceutical industry;
4) the ceramic membrane large-scale module has small negative amplification effect, and meanwhile, the module has wider material selection range during design, and has better adaptability in an application system sensitive to materials.
In a preferred embodiment, the step S500 further includes: filtering the filtrate B with ceramic membrane equipment to remove insoluble substances such as pectin, etc., setting the membrane temperature at 20-50 deg.C and pressure at 0.15-0.25Mpa, and collecting filtrate C.
In a preferred embodiment, the material of the organic film in step S600 may be one of cellulose derivatives, polysulfones, polyamides, polyimides, polyester-based polyolefins, silicon-containing polymers, and fluorine-containing polymers.
In a preferred embodiment, step S600 further includes:
s601, enabling the filtrate C to pass through a nanofiltration membrane system, setting the membrane passing temperature to be 20-50 ℃ and the membrane passing pressure to be 0.15-0.25Mpa, and collecting primary nanofiltration membrane trapped fluid;
s602, passing the primary nanofiltration membrane retentate through an ultrafiltration membrane, setting the membrane passing temperature to be 20-50 ℃ and the membrane passing pressure to be 0.15-0.25MPa, repeatedly replenishing water, filtering out oligosaccharide inorganic salt and the like, and measuring and collecting retentate D when the conductivity of the retentate is less than or equal to 500 mu S/cm and the sugar degree is less than or equal to 2%.
In a preferred embodiment, step S700 further includes: when the vacuum degree of the single-effect concentration equipment system reaches more than-0.05 Mpa, concentrating the collected trapped fluid D through the single-effect concentration equipment, setting the concentration temperature at 20-60 ℃, the pressure at 0.6-0.8Mpa, and when the concentration weight is 0.3-0.4 times of the weight of the dried medlar fruit raw material, measuring the sugar degree to be 5% -15% to obtain concentrated solution E.
In a preferred embodiment, step S800 further includes: and (3) freezing the concentrated solution E by a vacuum freeze dryer, carrying out primary sublimation, and carrying out analysis drying to obtain the lycium barbarum glycopeptide, wherein the freezing temperature is-35 to-40 ℃, the time is 3-4h, the primary sublimation temperature is 10-20 ℃, the time is 20-25h, the pressure is 30-35mbar, the analysis drying temperature is 20-30 ℃, the time is 7-11h, and the pressure is 15-20 mbar.
The following three examples illustrate how the method of making lycium barbarum glycopeptides of the present invention can be carried out.
Example 1
S100, weighing and cleaning the raw materials of the dried Chinese wolfberry fruits:
weighing dry wolfberry fruit raw materials and pouring the dry wolfberry fruit raw materials into a lifting machine, so that the dry wolfberry fruit raw materials are conveyed to a spray cleaning machine through the lifting machine and are cleaned by using purified water through vibration spray cleaning, wherein the quality of the weighed dry wolfberry fruit raw materials is not strictly limited in the claims of the application, and the purpose of explaining how much the quality of the weighed dry wolfberry fruit is determined according to the treatment capacity of post-equipment such as an aseptic storage tank, a vacuum freeze dryer and the like is also suitable for the embodiment 2 and the embodiment 3;
s200, leaching the cleaned dried Chinese wolfberry fruit raw material:
s201, pumping the cleaned dry Chinese wolfberry fruit raw materials into a primary sterile storage tank through a sanitary pump, adding purified water with the weight being 3 times that of the dry Chinese wolfberry fruit raw materials into the primary sterile storage tank, setting the temperature of the primary sterile storage tank at 30 ℃, stirring and soaking for 0.5h, and crushing the soaked dry Chinese wolfberry fruit raw materials through a crusher and a colloid mill to obtain primary Chinese wolfberry fruit raw pulp;
s202, pumping the primary wolfberry original pulp into a secondary sterile storage tank by a sanitary pump, adding purified water which is 3 times of the weight of the dry wolfberry raw materials weighed in the step S100 again, setting the temperature of the secondary sterile storage tank at 30 ℃, the stirring speed at 50r/min and the ultrasonic frequency at 20kHz, extracting for 1 hour to obtain and collect the wolfberry original pulp;
s300, removing the wolfberry seeds from the extracted wolfberry original pulp through a double-channel beater, and collecting pulp A:
s400, centrifuging the slurry A:
separating coarse fructus Lycii pulp from the pulp A at 3000f/f/min by horizontal screw centrifuge, separating fructus Lycii pulp from the filtrate B at 7000r/min by butterfly centrifuge, and collecting filtrate B;
s500, separating the filtrate B through an inorganic membrane, and collecting a filtrate C:
filtering the filtrate B with ceramic membrane equipment to remove insoluble substances such as pectin, setting the membrane temperature at 20 deg.C and pressure at 0.15Mpa, and collecting filtrate C;
s600, separating the filtrate C through an organic membrane, and collecting trapped fluid D:
s601, enabling the filtrate C to pass through a nanofiltration membrane system, setting the membrane passing temperature to be 20 ℃ and the membrane passing pressure to be 0.15Mpa, and collecting primary nanofiltration membrane trapped fluid;
s602, passing the primary nanofiltration membrane retentate through an ultrafiltration membrane again, setting the membrane passing temperature to be 20 ℃, the membrane passing pressure to be 0.15MPa, repeatedly replenishing water, filtering out oligosaccharide inorganic salt and the like, and collecting retentate D when the conductivity of the retentate is less than or equal to 500 mu S/cm and the sugar degree is less than or equal to 2% by determination;
s700, single-effect concentration of the trapped fluid D, and collection of a concentrated solution E:
when the vacuum degree of the single-effect concentration equipment system reaches more than-0.05 Mpa, concentrating the collected trapped fluid D by the single-effect concentration equipment, setting the concentration temperature at 20 ℃, the pressure at 0.6Mpa, and when the concentration weight is 0.3 times of the mass of the medlar raw material, measuring the sugar degree to be 5% to obtain a concentrated solution E;
s800, carrying out vacuum freeze drying on the concentrated solution E to obtain the lycium barbarum glycopeptide:
and (3) freezing the concentrated solution E by a vacuum freeze dryer, carrying out primary sublimation, and carrying out analytical drying to obtain the lycium barbarum glycopeptide, wherein the freezing temperature is-35 ℃, the time is 3h, the primary sublimation temperature is 10 ℃, the time is 25h, the pressure is 30mbar, the analytical drying temperature is 20 ℃, the time is 11h, and the pressure is 15 mbar.
Example 2
S100, weighing and cleaning the raw materials of the dried Chinese wolfberry fruits:
weighing dry Chinese wolfberry fruit raw materials, pouring the raw materials into a lifting machine, conveying the dry Chinese wolfberry fruit raw materials to a spray cleaning machine through the lifting machine, and cleaning the raw materials by using purified water through vibration spray;
s200, leaching the cleaned dried Chinese wolfberry fruit raw material:
s201, pumping the cleaned dry Chinese wolfberry fruit raw materials into a primary sterile storage tank through a sanitary pump, adding purified water with the weight being 4 times that of the dry Chinese wolfberry fruit raw materials into the primary sterile storage tank, setting the temperature of the primary sterile storage tank at 50 ℃, stirring and soaking for 0.7h, and crushing the soaked dry Chinese wolfberry fruit raw materials through a crusher and a colloid mill to obtain primary Chinese wolfberry fruit raw pulp;
s202, pumping into a secondary sterile storage tank by a sanitary pump, adding purified water which is 4 times of the weight of the medlar raw material weighed in the step S100 again, setting the temperature of the secondary sterile storage tank at 50 ℃, the stirring speed at 80r/min and the ultrasonic frequency at 25kHz, extracting for 0.7h to obtain and collect medlar original slurry;
s300, removing the wolfberry seeds from the extracted wolfberry original pulp through a double-channel beater, and collecting pulp A:
s400, centrifuging the slurry A:
separating coarse fructus Lycii pulp from the pulp A at 3500r/min by horizontal screw centrifuge, separating fructus Lycii pulp from the filtrate B at 7500f/f/min by butterfly centrifuge, and collecting filtrate B;
s500, separating the filtrate B through an inorganic membrane, and collecting a filtrate C:
filtering the filtrate B with ceramic membrane equipment to remove insoluble substances such as pectin, setting the membrane temperature at 35 deg.C and the pressure at 0.2Mpa, and collecting filtrate C;
s600, separating the filtrate C through an organic membrane, and collecting trapped fluid D:
s601, enabling the filtrate C to pass through a nanofiltration membrane system, setting the membrane passing temperature to be 35 ℃ and the membrane passing pressure to be 0.2Mpa, and collecting primary nanofiltration membrane trapped fluid;
s602, passing the primary nanofiltration membrane retentate through an ultrafiltration membrane again, setting the membrane passing temperature to be 35 ℃, the membrane passing pressure to be 0.2MPa, repeatedly replenishing water, filtering out oligosaccharide inorganic salt and the like, and collecting retentate D when the conductivity of the retentate is less than or equal to 500 mu S/cm and the sugar degree is less than or equal to 2% by determination;
s700, single-effect concentration of the trapped fluid D, and collection of a concentrated solution E:
when the vacuum degree of the single-effect concentration equipment system reaches more than-0.05 Mpa, concentrating the trapped fluid D by the single-effect concentration equipment, setting the concentration temperature at 45 ℃, the pressure at 0.7Mpa, and when the concentration weight is 0.35 times of the mass of the medlar raw material, measuring the sugar degree to be 10% to obtain a concentrated solution E;
s800, carrying out vacuum freeze drying on the concentrated solution E to obtain the lycium barbarum glycopeptide:
and (3) freezing the concentrated solution E by a vacuum freeze dryer, carrying out primary sublimation, and carrying out analytical drying to obtain the lycium barbarum glycopeptide, wherein the freezing temperature is-38 ℃, the time is 3.5h, the primary sublimation temperature is 15 ℃, the time is 22h, the pressure is 32mbar, the analytical drying temperature is 25 ℃, the time is 9h, and the pressure is 18 mbar.
Example 3
S100, weighing and cleaning the raw materials of the dried Chinese wolfberry fruits:
weighing dry Chinese wolfberry fruit raw materials, pouring the raw materials into a lifting machine, conveying the dry Chinese wolfberry fruit raw materials to a spray cleaning machine through the lifting machine, and cleaning the raw materials by using purified water through vibration spray;
s200, leaching the cleaned dried Chinese wolfberry fruit raw material:
s201, pumping the cleaned dry Chinese wolfberry fruit raw materials into a primary sterile storage tank through a sanitary pump, adding purified water with the weight 5 times that of the dry Chinese wolfberry fruit raw materials into the primary sterile storage tank, setting the temperature of the primary sterile storage tank at 80 ℃, stirring and soaking for 1 hour, and smashing the soaked dry Chinese wolfberry fruit raw materials through a pulverizer and a colloid mill to obtain primary Chinese wolfberry fruit raw pulp;
s202, pumping the primary wolfberry original pulp into a secondary sterile storage tank by a sanitary pump, adding purified water which is 5 times of the weight of the dry wolfberry raw materials weighed in the step S100 again, setting the temperature of the secondary sterile storage tank at 80 ℃, stirring speed at 100f/f/min and ultrasonic frequency at 30kHz, extracting for 0.5h to obtain and collect wolfberry original pulp;
s300, removing the wolfberry seeds from the extracted wolfberry raw pulp through a double-channel beater, and collecting pulp A:
s400, centrifuging the slurry A:
separating coarse fructus Lycii pulp from the pulp A at 4000f/f/min by horizontal decanter centrifuge, separating fructus Lycii pulp from the filtrate again at 8000f/f/min by butterfly centrifuge, and collecting filtrate B;
s500, separating the filtrate B through an inorganic membrane, and collecting a filtrate C:
filtering the filtrate B with ceramic membrane equipment to remove insoluble substances such as pectin, setting the membrane temperature at 50 deg.C and pressure at 0.25Mpa, and collecting filtrate C;
s600, separating the filtrate C through an organic membrane, and collecting trapped fluid D:
s601, enabling the filtrate C to pass through a nanofiltration membrane system, setting the membrane passing temperature at 50 ℃ and the membrane passing pressure at 0.25Mpa, and collecting primary nanofiltration membrane trapped fluid;
s602, passing the primary nanofiltration membrane retentate through an ultrafiltration membrane again, setting the membrane passing temperature at 50 ℃ and the membrane passing pressure at 0.25MPa, repeatedly replenishing water, filtering out oligosaccharide inorganic salt and the like, and collecting retentate D when the conductivity of the retentate is less than or equal to 500 mu S/cm and the sugar degree is less than or equal to 2% by determination;
s700, single-effect concentration of the trapped fluid D, and collection of a concentrated solution E:
when the vacuum degree of the single-effect concentration equipment system reaches more than-0.05 Mpa, concentrating the collected trapped fluid D by the single-effect concentration equipment, setting the concentration temperature at 60 ℃, the pressure at 0.8Mpa, and when the concentration weight is 0.4 times of the mass of the medlar raw material, measuring the sugar degree to be 15% to obtain a concentrated solution E;
s800, carrying out vacuum freeze drying on the concentrated solution E to obtain the lycium barbarum glycopeptide:
and (3) freezing the concentrated solution E by a vacuum freeze dryer, carrying out primary sublimation, and carrying out analytical drying to obtain the lycium barbarum glycopeptide, wherein the freezing temperature is-40 ℃, the time is 4h, the primary sublimation temperature is 20 ℃, the time is 20h, the pressure is 35mbar, the analytical drying temperature is 30 ℃, the time is 7h, and the pressure is 20 mbar.
The foregoing detailed description of the preferred embodiments of the invention has been presented. It should be understood that numerous modifications and variations could be devised by those skilled in the art in light of the present teachings without departing from the inventive concepts. Therefore, the technical solutions available to those skilled in the art through logic analysis, reasoning and limited experiments based on the prior art according to the concept of the present invention should be within the scope of protection defined by the claims.
Claims (10)
1. A preparation method of lycium barbarum glycopeptide comprises the following steps:
s100, weighing and cleaning the raw materials of the dried Chinese wolfberry fruits;
s200, leaching the cleaned dried Chinese wolfberry fruit raw material and collecting Chinese wolfberry fruit raw pulp;
s300, removing the wolfberry seeds from the original wolfberry pulp by a double-channel beater, and collecting pulp A;
s400, centrifuging the slurry A, and collecting filtrate B;
s500, separating the filtrate B through an inorganic membrane, and collecting a filtrate C;
s600, separating the filtrate C through an organic membrane, and collecting trapped fluid D;
s700, single-effect concentration is carried out on the trapped fluid D, and a concentrated solution E is collected;
s800, carrying out vacuum freeze drying on the concentrated solution E to obtain the lycium barbarum glycopeptide.
2. The method of claim 1, wherein, preferably, the step S100 further comprises: weighing the dried wolfberry fruit raw materials and pouring the dried wolfberry fruit raw materials into a lifting machine, conveying the dried wolfberry fruit raw materials to a spray cleaning machine through the lifting machine, and vibrating, spraying and cleaning the dried wolfberry fruit raw materials by using purified water.
3. The method of claim 1, wherein the step S200 further comprises the steps of:
s201, pumping cleaned dry Chinese wolfberry fruit raw materials into a primary sterile storage tank through a sanitary pump, adding purified water which is 3-5 times of the weight of the dry Chinese wolfberry fruit raw materials into the primary sterile storage tank, setting the temperature of the primary sterile storage tank at 30-80 ℃, stirring and soaking for 0.5-1h, and crushing the soaked dry Chinese wolfberry fruit raw materials through a crusher and a colloid mill to obtain primary Chinese wolfberry fruit raw slurry;
and S202, pumping the primary wolfberry original pulp into a secondary sterile storage tank by a sanitary pump, adding purified water which is 3-5 times of the weight of the dry wolfberry raw material weighed in the step S100 again, setting the temperature of the secondary sterile storage tank at 30-80 ℃, the stirring speed at 50-100r/min and the ultrasonic frequency at 20-30 kHz, extracting for 0.5-1h, and collecting the wolfberry original pulp.
4. The method of claim 1, wherein the step S400 further comprises: separating coarse fructus Lycii pulp from the slurry A by horizontal screw centrifuge at 3000-4000r/min, separating fructus Lycii pulp from the filtrate B by butterfly centrifuge at 7000-8000r/min, and collecting filtrate B.
5. The method of claim 1, wherein the inorganic membrane in step S500 is one of a metal membrane, an alloy membrane, a ceramic membrane, a molecular sieve composite membrane, a zeolite membrane, and a glass membrane.
6. The method of claim 5, wherein the step S500 further comprises: filtering the filtrate B with ceramic membrane equipment to remove insoluble substances such as pectin, etc., setting the membrane temperature at 20-50 deg.C and pressure at 0.15-0.25Mpa, and collecting filtrate C.
7. The method according to claim 1, wherein the organic film in step S600 is made of one of cellulose derivatives, polysulfones, polyamides, polyimides, polyester-based polyolefins, silicon-containing polymers, and fluorine-containing polymers.
8. The method of claim 1, wherein the step S600 further comprises:
s601, enabling the filtrate C to pass through a nanofiltration membrane system, setting the membrane passing temperature to be 20-50 ℃ and the membrane passing pressure to be 0.15-0.25Mpa, and collecting primary nanofiltration membrane trapped fluid;
s602, passing the primary nanofiltration membrane retentate through an ultrafiltration membrane, setting the membrane passing temperature to be 20-50 ℃ and the membrane passing pressure to be 0.15-0.25MPa, repeatedly replenishing water, filtering out oligosaccharide inorganic salt and the like, and determining and collecting retentate D when the conductivity of the retentate is less than or equal to 500 mu S/cm and the sugar degree is less than or equal to 2%.
9. The method of claim 1, wherein the step S700 further comprises: and when the vacuum degree of a single-effect concentration equipment system reaches more than-0.05 Mpa, concentrating the trapped fluid D by the single-effect concentration equipment, setting the concentration temperature to be 20-60 ℃, the pressure to be 0.6-0.8Mpa, and obtaining a concentrated solution E when the concentration weight is 0.3-0.4 time of the mass of the dried medlar fruit raw material and the sugar degree is 5-15%.
10. The method of claim 1, wherein the step S800 further comprises: and (3) freezing, sublimating for the first time, and analyzing and drying the concentrated solution E by a vacuum freeze dryer to obtain the lycium barbarum glycopeptide, wherein the freezing temperature is-35 to-40 ℃, the time is 3-4h, the sublimating temperature for the first time is 10-20 ℃, the time is 20-25h, the pressure is 30-35mbar, the analyzing and drying temperature is 20-30 ℃, the time is 7-11h, and the pressure is 15-20 mbar.
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| CN113501859A (en) * | 2021-07-01 | 2021-10-15 | 宁夏杞奕农业发展有限公司 | Lycium barbarum glycopeptide purification and standing equipment and use method thereof |
| CN114588250A (en) * | 2022-03-18 | 2022-06-07 | 宁夏杞肽科技有限公司 | Application of lycium barbarum glycopeptide in preparation of medicine for preventing or treating xerophthalmia |
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| Publication number | Priority date | Publication date | Assignee | Title |
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| CN112971127A (en) * | 2021-03-29 | 2021-06-18 | 陕西农产品加工技术研究院 | Medlar glycopeptide auxiliary blood pressure lowering oral liquid and preparation method thereof |
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| CN114588250B (en) * | 2022-03-18 | 2024-01-09 | 宁夏杞肽科技有限公司 | Application of lycium barbarum glycopeptide in preparing medicine for preventing or treating xerophthalmia |
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