CN110840806B - Kangaroo paw extract and preparation method and application thereof - Google Patents
Kangaroo paw extract and preparation method and application thereof Download PDFInfo
- Publication number
- CN110840806B CN110840806B CN201911285200.5A CN201911285200A CN110840806B CN 110840806 B CN110840806 B CN 110840806B CN 201911285200 A CN201911285200 A CN 201911285200A CN 110840806 B CN110840806 B CN 110840806B
- Authority
- CN
- China
- Prior art keywords
- kangaroo paw
- kangaroo
- extract
- micro
- extraction
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Active
Links
Images
Classifications
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K8/00—Cosmetics or similar toiletry preparations
- A61K8/18—Cosmetics or similar toiletry preparations characterised by the composition
- A61K8/96—Cosmetics or similar toiletry preparations characterised by the composition containing materials, or derivatives thereof of undetermined constitution
- A61K8/97—Cosmetics or similar toiletry preparations characterised by the composition containing materials, or derivatives thereof of undetermined constitution from algae, fungi, lichens or plants; from derivatives thereof
- A61K8/9783—Angiosperms [Magnoliophyta]
- A61K8/9794—Liliopsida [monocotyledons]
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61Q—SPECIFIC USE OF COSMETICS OR SIMILAR TOILETRY PREPARATIONS
- A61Q19/00—Preparations for care of the skin
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61Q—SPECIFIC USE OF COSMETICS OR SIMILAR TOILETRY PREPARATIONS
- A61Q19/00—Preparations for care of the skin
- A61Q19/02—Preparations for care of the skin for chemically bleaching or whitening the skin
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61Q—SPECIFIC USE OF COSMETICS OR SIMILAR TOILETRY PREPARATIONS
- A61Q19/00—Preparations for care of the skin
- A61Q19/08—Anti-ageing preparations
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K2800/00—Properties of cosmetic compositions or active ingredients thereof or formulation aids used therein and process related aspects
- A61K2800/80—Process related aspects concerning the preparation of the cosmetic composition or the storage or application thereof
- A61K2800/805—Corresponding aspects not provided for by any of codes A61K2800/81 - A61K2800/95
Abstract
The invention belongs to the technical field of plant extraction, and particularly relates to a kangaroo paw extract as well as a preparation method and application thereof. The invention provides a preparation method of a kangaroo paw extract, which comprises the following steps: mixing the kangaroo paw powder with an extraction solvent, and carrying out micro-jet extraction to obtain a kangaroo paw extract. According to the preparation method disclosed by the invention, the kangaroo paw is subjected to micro-jet extraction, the prepared kangaroo paw extract has high polyphenol content, the active ingredients are maximally reserved, and the kangaroo paw extract has remarkable whitening, soothing and antioxidant effects, can be widely applied to the field of cosmetics, so that the application range of the kangaroo paw plant which is mainly used for household ornamental flowers at present is expanded, and the market value of the kangaroo paw plant is increased. Moreover, the preparation method is easy to operate and suitable for industrial popularization and application.
Description
Technical Field
The invention belongs to the technical field of plant extraction, and particularly relates to a kangaroo paw extract as well as a preparation method and application thereof.
Background
Kangaroo paw (Anigozanthos flavidus) is a plant of the genus anthurium of the family haemacranthaceae (haemodora), which is native to southwestern australia and is typically characterized by a yellow or red tubular flower with dense villi on the surface, 6-split tip, shaped like a kangaroo paw, and is named. The developed underground rhizome of the kangaroo paw can enable the species to regenerate after drought or fire, and under a proper environment, a mature plant can grow more than 350 flowers and 10 long stems at most, so that the reproductive capacity is high. In addition, the kangaroo paw has the characteristics of drought resistance, sunshine preference, antibacterial property and the like, and the cross breeding success rate is high. There are about 50 cultivars worldwide, the second largest export flower species in australia, and at least 40 cultivars are exported as rare cut flowers to many countries in the world, such as the united states, israel and japan. 4 varieties are introduced from abroad to carry out trial planting and cultivation in 2002 by the flower research institute of the agricultural academy in Yunnan province of China, and the potential market value of kangaroo paw plants is urgently to be discovered.
At present, researches on the kangaroo paw are focused on seedling cultivation, tissue culture and the like, and the application number of patent CN201780063805.2, "kangaroo paw extract for beauty treatment" only discloses the application of the kangaroo paw extract as an anti-aging agent, and other effects and applications of the kangaroo paw extract are to be developed.
Disclosure of Invention
In view of the above, the invention provides a kangaroo paw extract, and a preparation method and application thereof.
The specific technical scheme of the invention is as follows:
a preparation method of kangaroo paw extract comprises the following steps:
mixing kangaroo paw powder with an extraction solvent, and carrying out micro-jet extraction to obtain a kangaroo paw extract.
Preferably, the extraction solvent is water and/or a lower alcohol;
the mass ratio of the extraction solvent to the kangaroo paw powder is 2-100: 1.
preferably, the temperature of the refrigerant extracted by the micro jet is 0-30 ℃;
the feeding speed of the micro-jet extraction is 5-30L/min.
Preferably, after the micro-jet extraction and before the obtaining of the kangaroo paw extract, the method further comprises the following steps:
and sequentially carrying out solid-liquid separation, impurity removal, purification and concentration.
Preferably, the solid-liquid separation is specifically solid-liquid separation by high-speed centrifugation;
the impurity removal specifically comprises the steps of removing impurities by adopting a microporous filter membrane;
the purification is specifically carried out by adopting an ultrafiltration membrane;
the concentration is specifically that the relative density is 1.010-1.200 when the membrane is concentrated to 25 ℃.
Preferably, the centrifugation speed of the high-speed centrifugation is 10000-20000 rpm, the centrifugation time of the high-speed centrifugation is 10-80 min, and the centrifugation frequency of the high-speed centrifugation is 1-2 times;
the aperture of the microporous filter membrane is 0.1-1 μm;
the aperture of the ultrafiltration membrane is 1000-15000 Da;
the operation pressure of the membrane concentration is 0.20-0.50 MPa, the flow rate of the membrane concentration is 20-50L/h, and the temperature of the membrane concentration is 20-40 ℃.
Preferably, the micro-jet extraction is performed by using a micro-jet extractor;
the microfluidic extractor includes: a microfluidic extraction unit;
the microfluidic extraction unit includes: the device comprises a shell, an inner gear ring and a micro jet ring;
the shell is a cylinder with a hollow structure;
the inner gear ring and the micro-jet ring are radially attached to the inner wall of the shell;
the inner gear ring is arranged at the working front end of the microjet ring without a gap;
the microjet ring partitions the housing into microjet extraction chambers;
the micro-jet ring is provided with a micro-jet hole.
The invention also provides a kangaroo paw extract which is prepared by the preparation method of the technical scheme.
The invention also provides application of the kangaroo paw extract in cosmetics.
Preferably, the cosmetic is in a dosage form selected from a cream, an emulsion, a water, a gel, a mask or a lotion;
the content of the kangaroo paw extract in the cosmetic is 0.1-100 wt%.
In summary, the invention provides a preparation method of kangaroo paw extract, which comprises the following steps: mixing kangaroo paw powder with an extraction solvent, and carrying out micro-jet extraction to obtain a kangaroo paw extract. The preparation method of the invention carries out micro-jet extraction on the kangaroo paw, the prepared kangaroo paw extract has high polyphenol content, the active ingredients are reserved to the maximum extent, the kangaroo paw extract has remarkable whitening, relieving and antioxidant effects, can be widely applied to the field of cosmetics, expands the application range of kangaroo paw plants which are mainly used for household ornamental flowers at present, increases the market value of the kangaroo paw plants, is easy to operate, and is suitable for industrial popularization and application.
Drawings
In order to more clearly illustrate the embodiments of the present invention or the technical solutions in the prior art, the drawings used in the description of the embodiments or the prior art will be briefly described below.
FIG. 1 is a schematic diagram of a microfluidic extractor according to the present invention;
FIG. 2 is a partial front view of a guide ring, an inner toothed ring, inner and outer toothed rings, and a microfluidic ring in a microfluidic extraction unit of the present invention;
FIG. 3 is a schematic view of a microjet ring provided in the present invention;
FIG. 4 is a time chart of the skin texture improvement effect of facial mask solution containing Kangaroo paw extract according to example 11, wherein the time intervals from the top first skin image are: vertically arranging and combining skin image recording diagrams at different times 0h before smearing, 1h after smearing, 2h after smearing and 4h after smearing;
FIG. 5 is a comparison of skin texture 0h before application and 4h after application of facial mask solution containing Kangaroo extract in example 11 of the present invention;
illustration of the drawings: 1. a drive unit; 2. a loop is hoisted; 3. a power interface; 4. a coupling; 5. a second refrigerant outlet; 6. a feed inlet; 7. a primary microfluidic extraction chamber; 8. a helical propulsion blade; 9. a secondary microfluidic extraction chamber; 10. a first refrigerant outlet; 11. a discharge port; 12. a tertiary microfluidic extraction chamber; 13. a rotating shaft; 14. cleaning a sewage discharge outlet; 15. a support; 16. a guide ring; 17. a first cooling ring; 18. a housing; 19. a first refrigerant inlet; 20. a second refrigerant inlet; 21. a second cooling ring; 22. a base; 23. a shock-absorbing plate; 711. an inner gear ring; 712. a micro-fluidic orifice; 713. a microjet ring; 714. an inner toothed ring and an outer toothed ring.
Detailed Description
The kangaroo paw extract prepared by the preparation method has high polyphenol content and has the effects of whitening, relieving and resisting oxidation.
The technical solutions in the embodiments of the present invention will be described clearly and completely below, and it is obvious that the described embodiments are only a part of the embodiments of the present invention, and not all of the embodiments. All other embodiments, which can be derived by a person skilled in the art from the embodiments given herein without making any creative effort, shall fall within the protection scope of the present invention.
A preparation method of kangaroo paw extract comprises the following steps:
mixing kangaroo paw powder with an extraction solvent, and carrying out micro-jet extraction to obtain a kangaroo paw extract.
According to the preparation method disclosed by the invention, the kangaroo paw extract is subjected to micro-jet extraction, the polyphenol content of the prepared kangaroo paw extract is high, the active ingredients are maximally reserved, the kangaroo paw extract has the effects of whitening, relieving and resisting oxidation, the effect is obvious, the application of the kangaroo paw extract can be expanded, and the market value of kangaroo paw plants is increased. Moreover, the preparation method is easy to operate and suitable for industrial popularization and application.
In the invention, the extraction solvent is water and/or lower alcohol;
the mass ratio of the extraction solvent to the kangaroo paw powder is 2-100: 1, preferably 5 to 100: 1.
in the invention, the temperature of the refrigerant extracted by the micro jet is 0-30 ℃, and preferably 10-20 ℃;
the feeding speed of the micro-jet extraction is 5-30L/min, preferably 15-30L/min.
In the present invention, the lower alcohol is propylene glycol or butylene glycol;
the propylene glycol is 20-70% of propylene glycol by mass;
the propylene glycol is 1, 2-propylene glycol and/or 1, 3-propylene glycol;
the butanediol is 1, 2-butanediol, 1, 3-butanediol and/or 1, 4-butanediol;
more preferably, the propylene glycol is 1, 2-propylene glycol and the butylene glycol is 1, 3-butylene glycol.
In the invention, after the micro-jet extraction and before the kangaroo paw extract is obtained, the method further comprises the following steps:
and sequentially carrying out solid-liquid separation, impurity removal, purification and concentration.
In the invention, solid-liquid separation is specifically carried out by adopting high-speed centrifugation;
the impurity removal specifically comprises the steps of removing impurities by adopting a microporous filter membrane;
the purification is specifically to adopt an ultrafiltration membrane for purification;
the concentration is specifically a relative density of 1.010 to 1.200 at 25 ℃ in the case of membrane concentration, and more preferably a relative density of 1.050 to 1.200 at 25 ℃ in the case of membrane concentration.
In the invention, the centrifugal speed of the high-speed centrifugation is 10000-20000 rpm, more preferably 14000-20000 rpm, and further preferably 14000-18000 rpm; the high-speed centrifugation time is 10-80 min, and more preferably 20-60 min; the centrifugation times of the high-speed centrifugation are 1-2 times;
the aperture of the microporous filter membrane is 0.1-1 μm, and more preferably 0.2-0.8 μm;
the aperture of the ultrafiltration membrane is 1000-15000 Da, and more preferably 2000-10000 Da;
the operation pressure of the membrane concentration is 0.20-0.50 MPa, and more preferably 0.30-0.45 MPa; the flow rate of membrane concentration is 20-50L/h, and more preferably 25-40L/h; the temperature of the membrane concentration is 20-40 ℃, and more preferably 20-30 ℃.
In the present invention, before mixing the kangaroo paw powder with the extraction solvent, the method further comprises: the dried or fresh kangaroo paw is sequentially sliced and crushed to obtain kangaroo paw powder, the mesh number of the kangaroo paw powder is 20-100 meshes, and the mesh number of the kangaroo paw powder is more preferably 50-90 meshes.
In the invention, the micro-jet extraction is carried out by adopting a micro-jet extractor.
Referring to fig. 1 to 3, fig. 1 is a schematic structural diagram of a micro-jet extractor in the present invention, fig. 2 is a partial front view of a guide ring, an inner gear ring, an outer gear ring and a micro-jet ring in a micro-jet extraction unit in the present invention, and fig. 3 is a schematic diagram of a micro-jet ring provided in the present invention.
In the present invention, the micro-jet extractor comprises: a microfluidic extraction unit;
the microfluidic extraction unit includes: housing 18, inner ring 711 and microjet ring 713;
the housing 18 is a cylinder having a hollow structure;
the inner toothed ring 711 and the microjet ring 713 are radially attached to the inner wall of the housing 18;
the inner toothed ring 711 is arranged at the working front end of the microjet ring 713 without a gap;
the microjet ring 713 is provided with microjet holes 712.
In the present invention, the microfluidic extraction unit further comprises: the inner and outer gear rings 714, the guide ring 16, the spiral propelling blade 8, the rotating shaft 13, the sealing ring and the first cooling ring 17;
the inner and outer toothed rings 714 are arranged in the inner toothed ring 711 and are coaxial with the inner toothed ring 711, and the inner and outer toothed rings 714 are fixed at the working front end of the micro jet ring 713;
the guide ring 16 is radially attached to the inner wall of the housing 18 and is located at the working front end of the inner ring 711 without a gap;
the inner diameter of the guide ring 16 decreases from the first end of the guide ring 16 to the second end of the guide ring 16;
the second end of the guide ring 16 is connected with the inner toothed ring 711;
the spiral propelling blade 8 and the rotating shaft 13 are arranged in the shell 18, and the spiral propelling blade 8 is fixed on the outer wall of the rotating shaft 13;
the rotating shaft 13 is positioned in the hollow part of the microfluidic ring 713 and is connected with the microfluidic ring 713 through a sealing ring;
the guide ring 16 is provided at the advancing direction end of the screw advancing blade 8;
the first cooling ring 17 is fitted over the outer wall of the housing 18.
It should be noted that the inner ring 711 is detachably and radially attached to the inner wall of the housing 18, and the inner ring 714 and the outer ring 714 are detachably fixed to the working front end of the microfluidic ring 713.
The guide ring 16 can guide the extracted materials transmitted by the spiral pushing blade 8, so that dead angles are avoided; the inner gear ring 711, the inner gear ring 714, the outer gear ring 714 and the micro-jet ring 713 also have a drainage function on the extracted materials; the first cooling ring 17 is disposed on the outer wall of the housing 18 and includes a first coolant inlet 19 and a first coolant outlet 10 for controlling the temperature of the kangaroo powder during the micro-jet extraction process.
In the present invention, the micro-jet extractor further comprises: a transmission unit and a drive unit 1;
the transmission unit comprises a coupling 4 and a second cooling ring 21;
the first end of the coupling 4 is connected to the shaft 13.
The second cooling ring 21 is sleeved on the outer wall of the coupling 4;
the second cooling ring 21 is provided with a second refrigerant inlet 20 and a second refrigerant outlet 5 for introducing a refrigerant to cool the rotating shaft 13;
the output shaft of the drive unit 1 is connected to the second end of the coupling 4.
In the invention, the diameter of the micro-jet hole 712 is 0.1-1.0 cm;
irregular protrusions are arranged on the wall of the micro jet hole 712;
the inner teeth of the inner ring 711 are irregular teeth;
the inner and outer teeth of the inner and outer rings 714 are irregular teeth.
The diameter of 0.1-1.0 cm of the micro-jet hole 712 is suitable for extraction and processing of kangaroo paw powder, and irregular protrusions on the wall of the micro-jet hole 712 have flocculation, cutting and other effects on extracted materials.
The number of the micro-jet extraction chambers is two or more.
In the present invention, the housing 18 is a hollow cylindrical container made of a pressure-resistant metal material, and the cleaning and sewage discharging outlet 14 is provided at the end of the housing 18 in the flowing direction after the tanaka is extracted.
When the kangaroo paw extract is prepared, the kangaroo paw extract is driven by the driving unit 1, the blades 8 are spirally pushed, so that huge centrifugal force is formed on kangaroo paw materials, the kangaroo paw materials instantly pass through the narrow micro-jet holes 712 to be secondarily accelerated to form high-frequency micro-jet, and the kangaroo paw materials are impacted and extruded at high speed under a pure physical state, are mixed by strong vortex and are subjected to strong vibration diffusion, so that histiocyte of the kangaroo paw materials is broken, low-temperature, rapid and full-component extraction is realized, the color, fragrance and taste of the kangaroo paw are kept, and the efficiency is extremely high.
In the invention, the number of the micro-jet extraction chambers is three, and the micro-jet extraction chambers comprise a primary micro-jet extraction chamber 7, a secondary micro-jet extraction chamber 9 and a tertiary micro-jet extraction chamber 12.
Further, the method also comprises the following steps: a damper sheet 23;
the driving unit 1 is disposed on a damper sheet 23, and the damper sheet 23 serves to reduce vibration and noise of the driving unit 1.
In the invention, the method also comprises the following steps: the drive unit 1 is provided with a support 15 and a base 22, the shell 18 is installed on the base 22 through the support 15, and the drive unit 1 is installed on the base 22 after being padded with a damping sheet 23.
In the present invention, the driving unit 1 includes a rotating electrical machine on which a suspension ring 2 is provided for easy installation and a power interface 3 is provided.
In the invention, a shell 18 is provided with a feed inlet 6 and a discharge outlet 11 for the inlet and outlet of a tower nano card material in a micro-jet extractor.
According to the invention, the microjet extraction is adopted, the temperature of a refrigerant extracted by the microjet is lower, the wall breaking can be more sufficient by the low-temperature microjet extraction, the low-temperature high-flow-rate extraction solvent is fully contacted with the dissolved components of the kangaroo paw, the contact area is larger, the heat-sensitive active components such as polyphenol, flavonoid, polysaccharide and the like can be maximally retained, and the impurities such as crude fiber and the like are removed by the operations of solid-liquid separation, purification and the like, so that the target components of the kangaroo paw can be rapidly, safely and accurately extracted, and the extract is safe and effective when being applied to cosmetics.
The kangaroo paw extract prepared by the preparation method disclosed by the invention is high in polyphenol content, and is shown to have remarkable capabilities of removing ABTS +. free radicals and DPPH free radicals through in-vitro efficacy test experiments, and the tyrosinase inhibition experiments, the hyaluronidase inhibition experiments and human skin test results further show that the kangaroo paw extract prepared by the preparation method disclosed by the invention is remarkable in effects of relieving, whitening, fading fine lines and resisting oxidation, so that the application of the kangaroo paw extract can be expanded, and the market value of kangaroo paw plants is increased.
The invention also provides a kangaroo paw extract which is prepared by the preparation method of the technical scheme.
The kangaroo paw extract has obvious effects of relieving, whitening, fading fine lines and resisting oxidation, can expand the application of the kangaroo paw extract and increase the market value of kangaroo paw plants.
The invention also provides the application of the kangaroo paw extract in cosmetics.
The kangaroo paw extract disclosed by the invention not only can enable cosmetics to have an anti-wrinkle effect, but also can enable the cosmetics to have remarkable whitening, relieving and antioxidant effects.
In the invention, the dosage form of the cosmetic is selected from cream, lotion, aqua, gel, mask or lotion;
the content of the kangaroo paw extract in the cosmetics is 0.1-100 wt%.
For a further understanding of the invention, reference will now be made in detail to the following examples.
In a particular embodiment, the kangaroo paw refers in particular to the aerial parts, including the flower and/or stem parts, of a fresh or dried kangaroo paw (Anigozanthos flavidus) plant.
Example 1
Mechanically pulverizing 5kg of fresh kangaroo paw, sieving with 80 mesh sieve to obtain kangaroo paw powder with average mesh number of 80 meshes, adding 150kg of purified water into the kangaroo paw powder, stirring, placing in a micro-jet extractor, and extracting at a refrigerant temperature of 20 deg.C and a feeding speed of 20L/min to obtain extractive solution. Pumping the extract into a high-speed centrifuge for solid-liquid separation at the centrifugal speed of 17000rpm, and collecting the separated liquid. And then pumping the separation liquid into a membrane treatment system, and sequentially removing impurities and purifying by adopting a 0.5-micron microporous filter membrane and a 7000Da ultrafiltration membrane to obtain the membrane separation liquid. And (3) performing membrane concentration on the membrane separation liquid under the conditions of 25 ℃ of temperature, 0.40-0.45 MPa of operation pressure and 25-30L/h of flow, and concentrating until the relative density of a concentrated liquid phase is 1.050(25 ℃), thus obtaining the kangaroo paw extract.
Example 2
Mechanically pulverizing 5kg of dried kangaroo paw, sieving with 80 mesh sieve to obtain kangaroo paw powder with average mesh number of 80 meshes, adding 150kg of purified water into the kangaroo paw powder, stirring, placing in a micro-jet extractor, and extracting at a refrigerant temperature of 20 deg.C and a feeding speed of 20L/min to obtain extractive solution. Pumping the extract into a high-speed centrifuge for solid-liquid separation at the centrifugal speed of 17000rpm, and collecting the separated liquid. And pumping the separation liquid into a membrane treatment system, and sequentially removing impurities and purifying by using a 0.5-micron microporous filter membrane and a 7000Da ultrafiltration membrane to obtain the membrane separation liquid. And (3) performing membrane concentration on the membrane separation liquid under the conditions of 25 ℃ of temperature, 0.40-0.45 MPa of operation pressure and 25-30L/h of flow, and concentrating until the relative density of a concentrated liquid phase is 1.050(25 ℃), thus obtaining the kangaroo paw extract.
Example 3
Mechanically pulverizing 5kg fresh kangaroo paw, sieving with 80 mesh sieve to obtain kangaroo paw powder with average mesh number of 80 mesh, adding 150kg purified water into kangaroo paw powder, stirring, placing in a micro-jet extractor, and extracting at refrigerant temperature of 30 deg.C and feeding speed of 20L/min to obtain extractive solution. Pumping the extract into a high-speed centrifuge for solid-liquid separation at the centrifugal speed of 17000rpm, and collecting the separated liquid. And then pumping the separation liquid into a membrane treatment system, and sequentially removing impurities and purifying by adopting a 0.5-micron microporous filter membrane and a 7000Da ultrafiltration membrane to obtain the membrane separation liquid. And (3) performing membrane concentration on the membrane separation liquid under the conditions of 25 ℃ of temperature, 0.40-0.45 MPa of operation pressure and 25-30L/h of flow, and concentrating until the relative density of a concentrated liquid phase is 1.050(25 ℃), thus obtaining the kangaroo paw extract.
Example 4
Mechanically pulverizing 5kg of fresh kangaroo paw, sieving with 80 mesh sieve to obtain kangaroo paw powder with average mesh number of 80 meshes, adding 150kg of purified water into the kangaroo paw powder, stirring, placing in a micro-jet extractor, and extracting at refrigerant temperature of 20 deg.C and feeding speed of 5L/min to obtain extractive solution. Pumping the extract into a high-speed centrifuge for solid-liquid separation at the centrifugal speed of 17000rpm, and collecting the separated liquid. And then pumping the separation liquid into a membrane treatment system, and sequentially removing impurities and purifying by adopting a 0.5-micron microporous filter membrane and a 7000Da ultrafiltration membrane to obtain the membrane separation liquid. And (3) performing membrane concentration on the membrane separation liquid under the conditions of 25 ℃ of temperature, 0.40-0.45 MPa of operation pressure and 25-30L/h of flow, and concentrating until the relative density of a concentrated liquid phase is 1.050(25 ℃), thus obtaining the kangaroo paw extract.
Comparative example 1
This comparative example is the same as example 1 except that the extraction method is an ultrasonic extraction method.
Mechanically pulverizing 5kg fresh kangaroo paw, sieving with 80 mesh sieve to obtain kangaroo paw powder with average mesh number of 80 meshes, adding 150kg purified water into the kangaroo paw powder, stirring, placing in ultrasonic extractor, and extracting at 20 deg.C and 40KHz for 30min to obtain extractive solution. Pumping the extract into a high-speed centrifuge for solid-liquid separation at the centrifugal speed of 17000rpm, and collecting the separated liquid. And then pumping the separation liquid into a membrane treatment system, and sequentially removing impurities and purifying by adopting a 0.5-micron microporous filter membrane and a 7000Da ultrafiltration membrane to obtain the membrane separation liquid. And (3) performing membrane concentration on the membrane separation liquid under the conditions of 25 ℃ of temperature, 0.40-0.45 MPa of operation pressure and 25-30L/h of flow, and concentrating until the relative density of a concentrated liquid phase is 1.050(25 ℃), thus obtaining the kangaroo paw extract.
Comparative example 2
This comparative example is the same as example 1 except that the extraction method is a decoction method.
Mechanically pulverizing 5kg of fresh kangaroo paw, sieving with 80-mesh sieve to obtain kangaroo paw powder with average mesh number of 80 meshes, adding 100kg of pure water into the kangaroo paw powder, stirring, placing in a heating kettle, decocting and extracting for 2h, filtering to obtain first extract, adding pure water which is 10 times of the weight of the original medicinal material into the residue, and decocting and extracting for 1h by the same method to obtain second extract. And combining the two extracting solutions, cooling, pumping into a high-speed centrifuge for solid-liquid separation at the centrifugal speed of 17000rpm, and collecting a separation solution. And then pumping the separation liquid into a membrane treatment system, and sequentially removing impurities and purifying by adopting a 0.5-micron microporous filter membrane and a 7000Da ultrafiltration membrane to obtain the membrane separation liquid. And (3) performing membrane concentration on the membrane separation liquid under the conditions of 25 ℃ of temperature, 0.40-0.45 MPa of operation pressure and 25-30L/h of flow, and concentrating until the relative density of a concentrated liquid phase is 1.050(25 ℃), thus obtaining the kangaroo paw extract.
Comparative example 3
This comparative example is the same as example 1 except that the extraction method is an enzymatic hydrolysis method.
Mechanically pulverizing 5kg of fresh kangaroo paw, sieving with 80-mesh sieve to obtain kangaroo paw powder with average mesh number of 80 meshes, adding 150kg of pure water into the kangaroo paw powder, stirring uniformly, placing in a heating kettle, adding cellulase at 60 deg.C and pH5.5, wherein the mass ratio of cellulase to kangaroo paw powder is 1: 50, stirring at constant temperature for 1h, and inactivating enzyme at 95 ℃ for 5min to obtain an extracting solution; cooling the extractive solution, pumping into high speed centrifuge at 17000rpm for solid-liquid separation, and collecting separated liquid. And then pumping the separation liquid into a membrane treatment system, and sequentially removing impurities and purifying by adopting a 0.5-micron microporous filter membrane and a 7000Da ultrafiltration membrane to obtain the membrane separation liquid. And (3) performing membrane concentration on the membrane separation liquid under the conditions of 25 ℃, 0.40-0.45 MPa of operating pressure and 25-30L/h of flow until the relative density of a concentrated liquid phase is 1.050(25 ℃), and thus obtaining the kangaroo paw extract.
Example 5
In this example, polyphenol content quantitative analysis was performed on kangaroo paw extracts of examples 1 to 4 and comparative examples 1 to 3, which specifically included:
1) preparation of control solution
Accurately weighing 0.110g of gallic acid monohydrate, adding purified water into a 100mL brown volumetric flask, dissolving, fixing the volume to the scale, and shaking up to obtain a gallic acid reference solution with the concentration of 1000 mug/mL.
2) Preparation of the Standard Curve
Gallic acid standard solution: 1.0mL, 2.0mL, 3.0mL, 4.0mL, and 5.0mL of gallic acid solution (1000. mu.g/mL) were pipetted into a 100mL volumetric flask, and the volume was fixed to a predetermined scale with water, followed by shaking (the concentrations were 10. mu.g/mL, 20. mu.g/mL, 30. mu.g/mL, 40. mu.g/mL, and 50. mu.g/mL, respectively).
The experimental process comprises the following steps: transferring 1.0mL of gallic acid standard solution and water into 10mL test tubes respectively by pipette, adding 5.0mL 10% Folin-Ciocalteu reagent into each test tube, shaking, reacting for 8min, adding 4.0mL 7.5% Na2CO3Adding water to the solution until the volume is constant to a scale mark (V), and shaking up. Standing at room temperature for 60 min. The corresponding absorbance was measured with a spectrophotometer at a wavelength of 765 nm. And drawing a standard curve by taking the concentration of each gallic acid standard solution as an abscissa and the absorbance of each standard solution as an ordinate.
3) Measurement method
1.0mL of the Kangaroo paw extracts of examples 1 to 4 and comparative examples 1 to 3 were precisely measured (if the concentration of polyphenol in the sample is high, 1.0mL of the extract should be diluted appropriately and used for color development measurement), the extract was placed in a 25mL volumetric flask to obtain a sample test solution, an experiment was performed according to the method under the "preparation of standard curve", the concentration (mg/mL) of polyphenol in the sample test solution was read from the standard curve, and the content of polyphenol in the sample was calculated by a calculation formula.
The polyphenol content of the sample was calculated as follows:
C＝N*V2/V1
in the formula:
c: the polyphenol content of the sample is mg/mL;
n: the concentration of polyphenol in the sample determination solution is obtained from the standard curve, and the unit is mg/mL;
V1: the measured volume of the sample is in mL.
V2: the volume of the sample is determined by the unit of mL;
the results retain a two decimal representation.
4) Analysis of results
As shown in table 1, it can be seen from table 1 that the polyphenol content of the kangaroo paw extract of examples 1 to 4 of the present invention is 80% to 200% higher than that of comparative examples 1 to 3, indicating that the extraction efficiency of polyphenol in the kangaroo paw extract is significantly improved by using the preparation method of the present invention to prepare the kangaroo paw extract.
TABLE 1 Polyphenol content in Kangaroo extract of examples 1-4 and comparative examples 1-3
Example 6
In the present embodiment, the antioxidant test, specifically the DPPH free radical scavenging test, is performed on the kangaroo paw extracts of examples 1 to 4 and comparative examples 1 to 3, and specifically includes:
1) formulation of DPPH reagent
0.00990g of DPPH (1,1-diphenyl-2-picrylhydrazyl) powder (with the molecular weight of about 394) is precisely weighed and placed in a 250mL volumetric flask, and a proper amount of 95% ethanol is used for dissolving the powder to reach the volume of 250mL, so that the DPPH reagent with the concentration of 0.10mmol/L is obtained.
2) Preparation of test article
The kangaroo paw extracts obtained in examples 1-4 and comparative examples 1-3 were added with 95% ethanol solution to a constant volume and prepared into 10.0mg/mL test solutions, respectively. A1.0 mg/mL vitamin E control solution was prepared in the same manner.
3) Measurement method
a. Adding 4.0ml DPPH solution and 1.0ml 95% ethanol in sequence into a 10ml test tube, mixing, shaking, reacting in dark for 30min, stabilizing, measuring absorbance at 517nm with 95% ethanol as reference, and recording as A0。
b. And sequentially adding 4.0ml of DPPH solution and 1.0ml of sample solution to be detected into a 10ml test tube, mixing and shaking uniformly, reacting for 30min in a dark place, and measuring the light absorption value at 517nm by taking 95% ethanol as a reference after stabilization, and recording as Ar.
c. Adding 4.0ml of 95% ethanol solution and 1.0ml of sample solution to be detected into a 10ml test tube in sequence, mixing and shaking uniformly, reacting for 30min in a dark place, and measuring the light absorption value at 517nm by taking 95% ethanol As a reference after stabilization, and recording As.
d. Calculating the formula: sample clearance of DPPH radicals:
the results obtained are expressed in percentages.
As shown in Table 2, the results of the tests show that the kangaroo paw extract of examples 1 to 4 of the present invention, when added in an amount of 10.0mg/mL, has an anti-radical effect relatively closer to that of 1.0mg/mL vitamin E (DPPH radical elimination rate at 1.0mg/mL concentration: 94%). Under the same test concentration, the DPPH free radical scavenging rate of the kangaroo paw extracts in the comparative examples 1-3 is only 10-40% of that of the kangaroo paw extracts in the examples 1-4, and the result shows that the DPPH free radical scavenging capacity of the kangaroo paw extracts prepared by the preparation method is higher.
TABLE 2 DPPH radical elimination ratio (10.0mg/mL) of Kangaroo extract in examples 1 to 4 and comparative examples 1 to 3
Example 7
In the embodiment, the antioxidant test, specifically the ABTS +. free radical scavenging experiment test, is performed on the kangaroo paw extracts in the embodiments 1 to 4 and the comparative examples 1 to 3, and specifically comprises the following steps:
1) reagent preparation
PBS solution: weighing 8g of NaCl, 0.2g of KCl and 1.44g of Na2HPO4And 0.24gKH2PO4The pH was adjusted to 7.2 and dissolved to 1000 mL.
b.2.45mmol/L Potassium persulfate: 100mL of the solution was prepared, and 0.0662g of potassium persulfate was taken to make 100mL of the solution with water.
c.7mmoL/L ABTS (2, 2' -Azinobis- (3-ethyllbenzhiazoline-6-sulfophosphate)) stock: dissolving 0.0384g of ABTS by using 2.45mmol/L potassium persulfate, keeping the volume to 10mL, keeping the solution dark green, standing for 12-16 h at room temperature in a dark place, and stabilizing the stock solution for 3-4 d.
d. Preparing ABTS +. determination solution: ABTS stock was diluted with phosphate buffer (pH 7.2) to reach an absorbance of 0.700 ± 0.020 at 734nm wavelength. When 0.12mmoL/L ABTS +. was prepared, the absorbance was measured at 0.716A (4 mL of LABTS +1mL of PBS) and diluted to 100mL using approximately 1.7mL of a 7mmoL L/LABTS stock.
2) Preparation of test article
The kangaroo paw extracts of examples 1-4 and comparative examples 1-3 were added with pure water to a constant volume and prepared into 10.0mg/mL solutions, respectively. A control solution of 5.0mg/mL vitamin C was prepared in the same manner as above.
3) Measurement method
a. 4mL of ABTS +. working solution was mixed with 1mL of sample for 10s, and then left to stand in the dark at 25 ℃ for 6min, and the absorbance was measured at 734 nm. Taking 4mL PBS +1mL sample as reference, note ASample (I)。
b. 4ml of ABTS +. working solution is mixed with 1ml of LPBS for 10s, then the mixture is kept stand for 6min in a dark place at 25 ℃, and the light absorption value is measured at 734 nm. Reference is made to PBS0。
Inhibition ratio (%) ((a)0-ASample (I)))/A0×100％
The results obtained are expressed in percentages.
4) Test results
The test results are shown in table 3, when the addition amount of the kangaroo paw extract in the embodiments 1 to 4 of the invention is 10.0mg/mL, the kangaroo paw extract has the relatively close effect of eliminating ABTS +. free radicals of 5.0mg/mL vitamin C, has very strong total inoxidizability, and can effectively reduce the activity of the free radicals having negative effects on the skin, prevent cells from releasing inflammatory mediators and protect epidermal cells in practical application. Under the same test concentration, the ABTS +. free radical clearance rate of the kangaroo paw extracts of the comparative examples 1-3 only reaches 40% -70% of that of the kangaroo paw extracts of the examples 1-4.
TABLE 3 ABTS +. radical scavenging Effect of Kangaroo paw extracts of examples 1-4 and comparative examples 1-3
| Test sample | ABTS +. radical scavenging Rate% |
| 5.0mg/mL vitamin C | 99 |
| 10.0mg/mL Kangaroo paw extract of example 1 | 96 |
| Example 2 10.0mg/mL Kangaroo paw extract | 87 |
| Example 3 10.0mg/mL Kangaroo paw extract | 93 |
| Example 4 10.0mg/mL Kangaroo paw extract | 91 |
| 10.0mg/mL Kangaroo paw extract of comparative example 1 | 60 |
| 10.0mg/mL Kangaroo paw extract of comparative example 2 | 44 |
| 10.0mg/mL Kangaroo paw extract of comparative example 3 | 38 |
Example 8
In the embodiment, the experimental test of tyrosinase inhibition is carried out on the kangaroo paw extracts of the embodiments 1 to 4 and the comparative examples 1 to 3, and the experimental test specifically comprises the following steps:
1) reagent preparation
Preparation of pbs (pH 6.8): accurately weighing 6.80g of monopotassium phosphate, and dissolving the monopotassium phosphate in 250mL of pure water to obtain 0.2mol/L potassium dihydrogen phosphate solution; 0.94g of sodium hydroxide was precisely weighed and dissolved in 118mL of pure water to obtain a 0.2mol/L sodium hydroxide solution. 250mL of 0.2mol/L potassium dihydrogen phosphate solution and 118mL of 0.2mol/L sodium hydroxide solution are mixed, and pure water is used for diluting to reach the constant volume of 1000mL, so that PBS (with the pH value of 6.8) is obtained.
Preparing an L-tyrosine solution: preparing 1.5 mmol/L-tyrosine solution with PBS, specifically dissolving L-tyrosine with a little 0.1mol/L HCl solution, and then diluting with PBS (pH 6.8) to pH 7.
c. Preparation of tyrosinase solution: dissolving with appropriate amount of PBS to obtain 200U/mL tyrosinase solution, dissolving tyrosinase with PBS (pH 6.8), subpackaging with PE tube, storing at-20 deg.C in refrigerator, taking out, and thawing at 4 deg.C in refrigerator.
2) Preparation of test article
The kangaroo paw extracts of examples 1-4 and comparative examples 1-3 were added with pure water to a constant volume and prepared into 10.0mg/mL solutions, respectively. 5.0mg/mL arbutin control solution was prepared in the same manner.
3) Measurement method
Precisely measuring 0mL of sample solution, 2.0mL of PBS and 1.0mL of tyrosinase solution, and uniformly mixing, and marking as A; precisely measuring 0mL of sample solution, 3.0mL of PBS and 0mL of tyrosinase solution, and mixing uniformly, and marking as B; precisely measuring 1.0mL of sample solution, 1.0mL of PBS and 1.0mL of tyrosinase solution, and uniformly mixing, and marking as C; precisely measuring 1.0mL of sample solution, 2.0mL of PBS and 0mL of tyrosinase solution, and uniformly mixing, and marking as D; mixing, keeping constant temperature in 37 deg.C water bath for 10min, adding 1.0mL of tyrosine solution, reacting for 15min, and measuring absorbance at 490 nm. A was read with B as reference and C with D as reference.
Calculating the formula: t ═ A-C)/A × 100%
A is the absorbance measured by the enzyme-added mixed liquor without the added sample;
b is the absorbance measured by the mixed solution without adding the sample and the enzyme;
c is the absorbance measured by the mixed solution of the sample and the enzyme;
d is the absorbance measured in the mixture with the sample and without the enzyme.
The results obtained are expressed in percentages.
4) Test results
The test results are shown in table 4, and when the addition amount of the kangaroo paw extract in the embodiments 1 to 4 is 10.0mg/mL, the kangaroo paw extract has a tyrosinase inhibition effect equivalent to 5.0mg/mL arbutin, and has a relatively significant whitening effect. Under the same test concentration, the tyrosinase inhibition rate of the kangaroo paw extract in the comparative examples 1-3 only reaches 30-50% of that of the kangaroo paw extract in the examples 1-4.
TABLE 4 tyrosinase inhibition rates of Kangaroo paw extracts of examples 1-4 and comparative examples 1-3
Example 9
In this example, hyaluronidase inhibition experiment tests are performed on kangaroo paw extracts of examples 1 to 4 and comparative examples 1 to 3, and specifically include:
1) reagent preparation
a. Preparation of acetic acid buffer solution (pH 5.6): weighing 1.155mL of glacial acetic acid to dilute to 100mL, and taking 4.8mL as A solution; weighing 2.72g of sodium acetate crystal, adding water to dissolve the crystal and fixing the volume to 100mL, and taking 45.2mL as a B solution; mixing the solution A and the solution B, and adding water to a constant volume of 100mL to mix uniformly. The pH value is measured precisely and adjusted to 5.6 with solution a (acidic) or solution B (basic).
b. Preparation of hyaluronidase solution: 10mg of hyaluronidase was weighed into a beaker and 4mL of acetic acid buffer was added at 1250 unit/mL.
c. Preparing a sodium hyaluronate solution: sodium hyaluronate 5.0mg was weighed and added to 10mL of acetic acid buffer solution to obtain a sodium hyaluronate solution (0.5 mg/mL).
d. Preparation of an erlipidemic reagent: 0.8g of p-dimethylaminobenzaldehyde is weighed out and dissolved in 15mL of concentrated hydrochloric acid and 15mL of absolute ethanol.
e. Preparing an acetylacetone solution: 3.5mL of acetylacetone was dissolved in 50mL of sodium carbonate solution (1.0 mol/L).
f. Preparing a sodium hydroxide solution: 16g of sodium hydroxide was weighed and dissolved in pure water, and the volume was adjusted to 1L with pure water.
2) Preparation of test article
The kangaroo paw extracts obtained in examples 1-4 and comparative examples 1-3 were added with pure water to a constant volume, and prepared into 15.0mg/mL solutions, respectively. And preparing 0.5mg/mL dipotassium glycyrrhizinate control solution by the same method.
3) Measurement method
0.1mL of 0.25mmol/L CaCl is taken2Incubating the solution and 0.5mL hyaluronidase solution (acetic acid buffer solution) at 37 ℃ for 20 min; adding 0.5mL of test solution (acetic acid buffer solution), and continuing to culture at 37 ℃ for 20 min; adding 0.5mL sodium hyaluronate solution, keeping the temperature at 37 ℃ for 30min, and standing at normal temperature for 5 min; adding 0.1mL of NaOH solution (0.4mol/L) and 0.5mL of acetylacetone solution, heating in boiling water bath for 15min, and immediately cooling with ice water for 5 min; adding 1.0mL of Ellisib reagent, diluting with 3.0mL of anhydrous ethanol, and standing for 20min for color development, wherein the reagents added to each group of samples are specifically shown in Table 5. First, the group A samples were subjected to UV-visible spectrophotometer
Wavelength scanning, recording the maximum absorption wavelength, and then using deionized water as a reference, ABS value determination is performed on each sample at the maximum absorption wavelength.
The calculation formula of the antiallergic activity is as follows:
hyaluronidase inhibition (%) - (A-B) - (C-D))/(A-B) × 100%
In the formula:
a-control solution ABS value (sample solution replaced with acetate buffer solution);
b-control blank solution ABS value (acetic acid buffer solution was used instead of sample solution and enzyme solution);
c is the ABS value of the sample solution;
D-ABS value of sample blank solution (acetic acid buffer solution is used to replace enzyme solution);
the results obtained are expressed in percentages.
TABLE 5 Hyaluronidase inhibition experiment Agents added to the samples
4) Test results
The test results are shown in table 6, and when the addition amount of the kangaroo paw extract in the embodiments 1 to 4 of the invention is 15.0mg/mL, the kangaroo paw extract has hyaluronidase inhibition effect close to 0.5mg/mL dipotassium glycyrrhizinate, which indicates that the kangaroo paw extract can effectively prevent histamine release and has certain allergy-relieving repair effect on skin. Under the same test concentration, the hyaluronidase inhibition rate of the kangaroo paw extracts of the comparative examples 1-3 only reaches 18% -40% of that of the kangaroo paw extracts of the examples 1-4 of the invention.
TABLE 6 hyaluronidase inhibition ratios of kangaroo paw extracts of examples 1-4 and comparative examples 1-3
Example 10
This example provides a cream comprising the extract of kangaroo paw of example 1, the formulation of which is shown in table 7, and the cream of this example was prepared as follows:
weighing an A1 phase, dispersing and dissolving the A1 phase in advance until the phase is transparent, adding an A2 phase, heating to 80-85 ℃, and homogenizing for 3 min; weighing the phase B, and heating and stirring to 80-85 ℃; adding phase B into phase A, stirring, homogenizing for 5min, and stirring for 20 min; cooling to 45 deg.C under stirring, adding C, D phase, and stirring.
TABLE 7 EXAMPLE 10 formulation of cream
Example 11
The present example provides a facial mask solution comprising the kangaroo paw extract of example 1, the formula of the facial mask solution is shown in table 8, and the preparation method of the facial mask solution of the present example is as follows:
weighing the phase A raw material, uniformly mixing the phase A and ensuring that all components are completely dissolved, heating to 70-75 ℃, preserving heat for 20min, cooling to 45 ℃ while stirring, adding the phase B, and uniformly stirring to obtain the composition.
Table 8 example 11 formulation of facial mask solution
Example 12
The present example provides a serum containing the extract of kangaroo paw of example 1, the formula of the serum is shown in table 9, and the preparation method of the serum of this example is as follows:
weighing the phase A raw material, uniformly mixing the phase A and ensuring that all components are completely dissolved, heating to 60-65 ℃, and preserving heat for 20 min. Cooling to 45 deg.C under stirring, adding phase B, and stirring.
Table 9 formula of essence of example 12
Example 13
The embodiment adopts the facial mask solution of embodiment 11 to perform the skin texture improvement test of the human body, and specifically comprises the following steps:
1) a subject: 13 men and 18 women, 31 people in total, and the age is 18-35 years.
2) Test article: example 11 of the present invention includes facial mask solution containing kangaroo paw extract and pure water.
3) The test method comprises the following steps: taking a range of 2 x 2cm at the arm curve side of a subject, lightly wiping the test area with clean water before testing, and detecting and recording skin appearance images of the tested area of the left hand and the right hand of the subject by using a skin detector (CBS-805) after 2 hours; then 1.0mL of the facial mask solution of example 11 containing kangaroo paw extract was applied to the left-hand test area, followed by 1.0mL of pure water in the same manner to the right-hand test area, and image recordings were made at regular intervals for 4 hours.
4) And (4) analyzing results: FIG. 4 is a time chart of the skin texture improvement effect of facial mask solution containing Kangaroo paw extract according to example 11, wherein the time intervals from the top first skin image are: the skin image recording images are vertically arranged and combined at different time periods after 0h before smearing, 1h after smearing, 2h after smearing and 4h after smearing. Fig. 5 is a comparison graph of skin texture 0h before and 4h after application of facial mask solution containing kangaroo paw extract in example 11 of the present invention, and fig. 4 and 5 show that skin wrinkles are significantly reduced, lightened, and the effect can be continuously maintained after application of facial mask solution containing kangaroo paw extract in example 11 of the present invention.
The foregoing is only a preferred embodiment of the present invention, and it should be noted that, for those skilled in the art, various modifications and decorations can be made without departing from the principle of the present invention, and these modifications and decorations should also be regarded as the protection scope of the present invention.
Claims (6)
1. The preparation method of the kangaroo paw extract is characterized by comprising the following steps:
mixing kangaroo paw powder with an extraction solvent, and carrying out micro-jet extraction to obtain a kangaroo paw extract;
the extraction solvent is water;
the mass ratio of the extraction solvent to the kangaroo paw powder is 2-100: 1;
the temperature of the refrigerant extracted by the micro jet is 0-30 ℃;
the feeding speed of the micro-jet extraction is 5-30L/min;
the micro-jet extraction is to adopt a micro-jet extractor for extraction;
the microfluidic extractor includes: a microfluidic extraction unit;
the microfluidic extraction unit includes: the device comprises a shell, an inner gear ring and a micro jet ring;
the shell is a cylinder with a hollow structure;
the inner gear ring and the micro-jet ring are radially attached to the inner wall of the shell;
the inner gear ring is arranged at the working front end of the microjet ring without a gap;
the microjet ring partitions the housing into microjet extraction chambers;
the micro-jet ring is provided with a micro-jet hole.
2. The method of claim 1, wherein after the microfluidic extraction and before the obtaining of the kangaroo paw extract, the method further comprises:
sequentially carrying out solid-liquid separation, impurity removal, purification and concentration;
the solid-liquid separation is specifically carried out by adopting high-speed centrifugation;
the impurity removal specifically comprises the steps of removing impurities by adopting a microporous filter membrane;
the purification is specifically carried out by adopting an ultrafiltration membrane;
the concentration is specifically that the relative density is 1.010-1.200 when the membrane is concentrated to 25 ℃.
3. The method according to claim 2, wherein the centrifugation speed of the high-speed centrifugation is 10000 to 20000rpm, the centrifugation time of the high-speed centrifugation is 10 to 80min, and the number of times of the high-speed centrifugation is 1 to 2;
the aperture of the microporous filter membrane is 0.1-1 mu m;
the aperture of the ultrafiltration membrane is 1000-15000 Da;
the operation pressure of the membrane concentration is 0.20-0.50 MPa, the flow rate of the membrane concentration is 20-50L/h, and the temperature of the membrane concentration is 20-40 ℃.
4. A kangaroo paw extract, which is produced by the production method according to any one of claims 1 to 3.
5. Use of the kangaroo paw extract of claim 4 in cosmetics.
6. Use according to claim 5, characterized in that the cosmetic formulation is selected from a cream, an emulsion, a water aqua, a gel, a mask or a lotion;
the content of the kangaroo paw extract in the cosmetic is 0.1-100 wt%.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201911285200.5A CN110840806B (en) | 2019-12-13 | 2019-12-13 | Kangaroo paw extract and preparation method and application thereof |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201911285200.5A CN110840806B (en) | 2019-12-13 | 2019-12-13 | Kangaroo paw extract and preparation method and application thereof |
Publications (2)
| Publication Number | Publication Date |
|---|---|
| CN110840806A CN110840806A (en) | 2020-02-28 |
| CN110840806B true CN110840806B (en) | 2022-07-12 |
Family
ID=69609078
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN201911285200.5A Active CN110840806B (en) | 2019-12-13 | 2019-12-13 | Kangaroo paw extract and preparation method and application thereof |
Country Status (1)
| Country | Link |
|---|---|
| CN (1) | CN110840806B (en) |
Families Citing this family (4)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN115025010A (en) * | 2021-03-04 | 2022-09-09 | 新生活化妆品科技(上海)有限公司 | Application of kangaroo paw extract and cosmetic composition for improving expression lines |
| CN113208961A (en) * | 2021-05-08 | 2021-08-06 | 广州市络捷生物科技有限公司 | Preparation method and application of trollius chinensis bunge extract |
| CN113509412B (en) * | 2021-05-08 | 2023-06-27 | 广州市络捷生物科技有限公司 | Preparation method and application of sausage fruit extract |
| CN114588089B (en) * | 2022-03-31 | 2024-01-12 | 广州市络捷生物科技有限公司 | Plant extract and preparation method and application thereof |
Citations (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| WO2015074120A1 (en) * | 2013-11-22 | 2015-05-28 | The University Of Queensland | Nanocellulose |
| CN107669557A (en) * | 2017-11-08 | 2018-02-09 | 广州市络捷生物科技有限公司 | A kind of Ta Naka extracting methods |
| CN109937031A (en) * | 2016-10-17 | 2019-06-25 | 国际香料香精公司 | Kangaroo pawl extract for beautifying use |
-
2019
- 2019-12-13 CN CN201911285200.5A patent/CN110840806B/en active Active
Patent Citations (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| WO2015074120A1 (en) * | 2013-11-22 | 2015-05-28 | The University Of Queensland | Nanocellulose |
| CN109937031A (en) * | 2016-10-17 | 2019-06-25 | 国际香料香精公司 | Kangaroo pawl extract for beautifying use |
| CN107669557A (en) * | 2017-11-08 | 2018-02-09 | 广州市络捷生物科技有限公司 | A kind of Ta Naka extracting methods |
Non-Patent Citations (1)
| Title |
|---|
| 微射流提取技术在天然植物抗过敏活性成分中的应用研究;黄裕 等;《药物研究》;20161231(第12期);第25-27页 * |
Also Published As
| Publication number | Publication date |
|---|---|
| CN110840806A (en) | 2020-02-28 |
Similar Documents
| Publication | Publication Date | Title |
|---|---|---|
| CN110840806B (en) | Kangaroo paw extract and preparation method and application thereof | |
| CN107669557B (en) | Tanaka extraction method | |
| CN101284022B (en) | Complexation extraction method of plant polyphenols substances | |
| US10479774B2 (en) | Method for separating flavonoid substances in Camellia nitidissima Chi based on a magnetic nanoparticles-PAMAM nano composites | |
| CN103265520B (en) | Method for preparing oligomeric proanthocyanidins and tannin pigment from grape seeds after winemaking | |
| CN101891580B (en) | Method for supercritical carbon dioxide extraction of lycopene in watermelon | |
| CN101961427A (en) | Method for extracting polysaccharide and polyphenol of oil-tea camellia husk | |
| CN101575628A (en) | Method for preparing tea polysaccharide through separation and purification and structural identification thereof | |
| CN104059163B (en) | Qaidam, Qinghai lycium barbarum polysaccharide is extracted and is separated novel preparation method | |
| CN106581501B (en) | A method of extracting general flavone from rice-pudding leaf | |
| CN106222226A (en) | The method preparing bata-carotene crystal | |
| CN108997294A (en) | High-quality glucosidase procyanidins and preparation method thereof | |
| CN103788227A (en) | Extraction method for isatis root polysaccharides | |
| CN109010504A (en) | A kind of extracting method of lycium ruthenicum general flavone | |
| CN108815036A (en) | A kind of preparation method and applications of Ta Naka extract | |
| CN106278975A (en) | The method preparing bata-carotene crystal | |
| CN102942606B (en) | Method for preparing high-purity lycium barbarum acid | |
| CN114224943A (en) | Aronia melanocarpa extract and preparation method thereof | |
| CN104644715A (en) | Combined-enzyme method extraction process for wild chrysanthemum flower flavonoids compound | |
| CN102604540B (en) | A kind of series tannin extract raw material subtle powder smash is prepared and for series tannin extract product mix, couplingization preparation technology and method | |
| CN109432302A (en) | A kind of extracting method of dendrobium candidum phenol | |
| CN107714903A (en) | The extraction technique of flavone compound in a kind of garlic | |
| CN102641316B (en) | Method for extracting bioactive substances from marigold slag through subcritical water extraction technology | |
| CN101311225A (en) | Process for abstracting kohlrabi pigment | |
| CN111592578B (en) | Process for extracting tea saponin from camellia sinensis dregs and application of tea saponin in amino acid facial soap |
Legal Events
| Date | Code | Title | Description |
|---|---|---|---|
| PB01 | Publication | ||
| PB01 | Publication | ||
| SE01 | Entry into force of request for substantive examination | ||
| SE01 | Entry into force of request for substantive examination | ||
| GR01 | Patent grant | ||
| GR01 | Patent grant |