CN110819542B - Mucospora viscidula, microbial inoculum comprising Mucospora viscidula and application - Google Patents

Mucospora viscidula, microbial inoculum comprising Mucospora viscidula and application Download PDF

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CN110819542B
CN110819542B CN201911248822.0A CN201911248822A CN110819542B CN 110819542 B CN110819542 B CN 110819542B CN 201911248822 A CN201911248822 A CN 201911248822A CN 110819542 B CN110819542 B CN 110819542B
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刘玮
赵小敏
张嵚
李金苗
薛华健
张金莲
吴佳海
邓光华
张微微
李响
张露
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Abstract

The invention provides a strain of glomus viscus, a microbial inoculum comprising the glomus viscus and application, and belongs to the technical field of microbial preparations. The mucose sacculus isolariciresis Sv-01 can form a mutual-benefiting symbiont with plants, so that the growth of the plant container seedlings is remarkably promoted, the nutritional status of the plant container seedlings is improved, and the capacity of the plant container seedlings for resisting external stress is improved.

Description

Mucospora viscidula, microbial inoculum comprising Mucospora viscidula and application
Technical Field
The invention relates to the technical field of microbial preparations, in particular to a mucosepalax sacculus mildew, a microbial inoculum comprising the mucosepalax sacculus mildew and application of the mucosepalax sacculus mildew.
Background
Arbuscular Mycorrhizal Fungi (AMF) form a reciprocal symbiont with a plant nutrient root system to promote the absorption of nutrients such as host plant N, P, Cu and Zn, improve the permeability and water holding capacity of soil, enhance the drought resistance of plants, increase the accumulation of organic carbon in soil, improve the stress resistance of plants to stress such as water and nutrient deficiency, improve the growth of plants, improve the biomass of plants and the like. Glomus mucosae (Claroideis clarioides) is one of arbuscular mycorrhizal fungi, but few glomus mucosae with plant growth promoting effect are published in the prior art, and particularly, a glomus mucosae with extensive plant growth promoting effect is lacked.
Disclosure of Invention
The invention aims to provide a strain of mucosepalax sacculus, a microbial inoculum comprising the mucosepalax sacculus and application of the mucosepalax sacculus.
In order to achieve the above object, the present invention provides the following technical solutions:
the invention provides a strain of glomus septemlobus viscosum Sv-01 with a preservation number of CGMCC No. 18594.
The invention also provides a microbial inoculum of the mucosacculus isolaricius in the scheme.
Preferably, the effective spore number of the glomus pusillus in the microbial inoculum is 10-80/g.
The invention also provides application of the mucose sacculus isolariceus or the microbial inoculum in the scheme in promoting growth of plant container seedlings, promoting plant photosynthesis and/or promoting plant transpiration, and the method comprises the following steps: filling matrix and microbial inoculum in the container from bottom to top in sequence, planting plant seedling, covering the matrix, watering, fixing seedling after the plant seedling grows stably, and culturing.
Preferably, the plant comprises euscaphis konishii hayata, cinnamomum kohlrabi or toona sinensis.
Preferably, the matrix comprises the following raw materials in parts by volume: 2.8-3.2 parts of garden soil, 0.8-1.2 parts of grass carbon and 0.8-1.2 parts of river sand.
Preferably, the container comprises a plastic flower pot.
Preferably, the number of seedlings per container is 1-2.
Preferably, the optical density of the culture is 750-850 mmol · m-2·s-1The illumination time is 15.5-16.5 h.d-1The temperature is 25-27 ℃, and the humidity is 62-68%.
The invention has the beneficial effects that: the invention provides a strain of glomus septemlobus viscosum Sv-01 with a preservation number of CGMCC No. 18594. The mucose sacculus isolariciresis Sv-01 can form a mutual-benefiting symbiont with plants, so that the growth of the plant container seedlings is remarkably promoted, the nutritional status of the plant container seedlings is improved, and the capacity of the plant container seedlings for resisting external stress is improved. Through application, the mucoid sacculus isolariciressa Sv-01 can remarkably promote the growth of the overground part of a plant container seedling, increase the number of leaves, the plant height and the ground diameter of the plant container seedling, increase the total root length, the root surface area and the number of root tips of the plant container seedling, increase the dry weight of the root, the stem weight, the leaf dry weight and the total dry weight of the plant container seedling, improve the absorption efficiency of the plant on nutrients, assist the plant to absorb the nutrients, promote the growth of the plant, promote the photosynthesis and the transpiration of the plant and improve the stress resistance of the plant.
Biological preservation Instructions
Clarias claroides Sv-01 is preserved in China general microbiological culture Collection center (CGMCC) 10.14 days 2019 at the address of No. 3 of the national institute of sciences, institute of microbiology, China, with the preservation number: CGMCC No. 18594.
Detailed Description
The invention provides a Claroideidoglomus claroideium Sv-01 strain with the preservation number of CGMCC No. 18594; the mucose sacculus isolariceus Sv-01 is separated from moso bamboo rhizosphere soil (the elevation is 740 meters) in the golden bamboo lawn area of the Lushan national-level natural protection area in Jiangxi province. In the invention, the glomus mucosae and the host plant form a good symbiotic structure, and the growth of the host plant is promoted by promoting the nutrient absorption and improving the photosynthetic condition of the glomus mucosae.
In the invention, the spores of the mucosacculus mucosae have the following characteristics: a plurality of spores grow in plant root soil in clusters, the spores are spherical or nearly spherical, translucent white to the yellow color of rice straw, the average value is 77.76 μm, the surfaces of the spores are usually covered with debris, and the color becomes dark and dull. Spore wall: 3 layers, wherein the L1 layer is a flexible wall with the thickness of 1.0-2.0 μm, and the thickness of the surface is about 2.0-5.0 μm after being covered by soil particles or other substances; when mature spores are crushed, the L1 layer is easily separated from the L2 layer, and the layer of young tender spores is not easy to distinguish; the coloration was not evident in the Melzer's reagent staining reaction and was light blue in the cotton blue reagent reaction. The L2 layer is transparent film with thickness of 0.5-1.0 μm, and only a few spores can be observed when the spores are broken. The layer thickness of L3 is 2.08-4.37 μm, and the layer wall is transparent, and the layer wall is light yellow to light yellow brown, and the layer wall is light blue in the reaction of cotton blue reagent, and the reaction in Melzer's reagent is not obvious. Hypha of Neurospora: the single root is straight or slightly inclined, the cylinder shape is occasionally slightly constricted, the width of the connecting point is 8.0-11.0 mu m, the outer layer of the mature spore is usually fallen off, the inner layer is connected with the spore wall L3, and the isolating and sealing connecting point formed by the inner wall is occasionally arranged.
Amplifying single spores of the mucose sacculus isolariceus Sv-01 by adopting nested PCR, wherein the first PCR amplification adopts a fungus universal primer pair GeoA2-Geo 11; the second PCR amplification used the AM fungal specific universal primer pair AML1-AML 2. The 18S rDNA nucleotide sequence sequenced by the Sv-01 spore of the glomus pusillus is shown as SEQ ID NO: 1, specifically: 5' -GGG ACC TCA TCA GCT CAT AGC GTA TAT TAA AGT TGT TGC AGT TAA AAA GCT CGT AGT TGA ACT TCG GGA TCA ATG GAT TGG TCA TGC CGA TGG TGT GTA CTG GTC ATA TTG GTT CTC ACC TTC TAA GGA ACC ATAATG TCA TTT ATT TGA TGT TGT GGG GAA TTA GGA CTG TTA CCT TGA AAA AAT TAG AGT GTT TAA AGC AGG CTA ACG CTT GAA TAC ATT AGC ATG GAA TAA TGA AAT AGG ACA TCG CAT TCT ATT TTG TTG GTT TCT AGG ATC GAT GTA ATG ATT AAT AGG GAT AGT TGG GGG CAT TAG TAT TCA ATT GTC AGA GGT GAA ATT CTT GGA TTT ATT GAA GAC TAA CTA CTG CGA AAG CAT TTG CCA AGG ATG TTT TCA TTA ATC AAG AAC GAA AGT TAG GGG ATC GAA GAC GAT CAG ATA CCG TCG TAG TCT TAA CCA TAA ACT ATG CCG ACT AGG GAT CGG ATGATG TTG ATT TTT TAA TGA CTC ATT CGG CAC CTT ACG GGA AAC CAA AGT GTT TGG GTT CAA TA-3'.
The invention also provides a microbial inoculum of the mucosacculus isolaricius in the scheme; the effective number of spores of the glomus incarnatum in the microbial inoculum is preferably 10-80/g, more preferably 20-70/g, and most preferably 30-50/g.
In the invention, the microbial inoculum is preferably prepared by the following method: inoculating the glomus incanus into a matrix to obtain an expanding propagation matrix, planting corn and/or clover by using the expanding propagation matrix, fully propagating the glomus incanus between the expanding propagation matrix and a plant (corn and/or clover) root system, and finally harvesting a microbial inoculum consisting of dry soil of the expanding propagation matrix, hyphae outside the root, hyphae inside the root and an infected root section; the substrate comprises river sand and garden soil; the mass ratio of the river sand to the garden soil is preferably 1: 1; the substrate is preferably sterilized.
The invention also provides application of the mucose sacculus isolariceus or the microbial inoculum in the scheme in promoting growth of plant container seedlings, promoting plant photosynthesis and/or promoting plant transpiration, and the method comprises the following steps: filling a substrate and a microbial inoculum in a container from bottom to top in sequence, planting plant seedlings, then covering the substrate, watering, fixing seedlings after the plant seedlings grow stably, and culturing; the plant preferably comprises euscaphis konishii hayata, cinnamomum kohlrabi or toona sinensis.
In the invention, the application of the microbial inoculum in promoting the growth of the plant container seedlings preferably comprises promoting the overground part growth of the plant container seedlings and/or increasing the leaf number, the plant height and the ground diameter of the plant container seedlings and/or increasing the total root length, the root surface area and the root tip number of the plant container seedlings and/or increasing the dry weight, the stem weight, the leaf dry weight and the total dry weight of the plant container seedlings.
In the inventionThe matrix preferably comprises the following raw materials in parts by volume: 2.8-3.2 parts of garden soil, 0.8-1.2 parts of turf and 0.8-1.2 parts of river sand, and more preferably, the matrix comprises the following raw materials in parts by volume: 3 parts of garden soil, 1 part of grass peat and 1 part of river sand; the substrate is preferably screened through a 2mm screen; the pH value of the substrate is preferably 5.5-6.5, and more preferably 5.99; the organic matter content of the substrate is preferably 35-45 g-kg-1More preferably 40 g/kg-1(ii) a The organic carbon content of the substrate is preferably 65-75 g/kg-1More preferably 68 g/kg-1(ii) a The total phosphorus content of the substrate is preferably 0.5-0.9 g-kg-1More preferably 0.8 g/kg-1(ii) a The total nitrogen content of the substrate is preferably 1.7-2.1 g/kg-1More preferably 1.9 g/kg-1(ii) a The content of ammonium nitrogen in the matrix is preferably 13-18 mg/kg-1More preferably 15 mg/kg-1(ii) a The preferable content of nitrate nitrogen in the matrix is 4-4.5 mg/kg-1More preferably 4.3 mg/kg-1。
In the present invention, the matrix is preferably prepared by the following method: mixing the garden soil, the grass peat and the river sand, sieving the mixture by a 2mm sieve, and sterilizing the mixture to obtain a matrix; the mixing method is not particularly limited, and the uniform mixing is taken as the standard; the sterilization temperature is preferably 115-130 ℃, and more preferably 121 ℃; the pressure of the sterilization is preferably 0.1 MPa; the sterilization time is preferably 0.3-0.8 h, and more preferably 0.5 h; the sterilization mode is preferably high-temperature high-pressure steam sterilization.
In the present invention, the container preferably comprises a plastic flowerpot; the upper caliber, the lower caliber and the height of the plastic flowerpot meet the requirements of the planted plants.
In the invention, the container with the specification of upper caliber multiplied by lower caliber multiplied by high respectively being 9 multiplied by 6 multiplied by 8cm or the container with the specification of upper caliber multiplied by lower caliber multiplied by high respectively being 16 multiplied by 11 multiplied by 12.5cm (plastic flowerpot) is taken as a standard, the mass of the filling substrate of each container is preferably 60 to 300g, and more preferably 80 to 180 g; the mass of the filled microbial inoculum is preferably 50-70 g, and more preferably 60 g; the mass of the covering substrate is preferably 50-120 g, and more preferably 60-100 g; the number of the implanted plant seedlings is preferably 2-4, more preferably 3; the amount of water watered in each container is preferably 150-250 mL, and more preferably 200 mL; the number of seedlings per container is preferably 1-2. The method for obtaining the young plant is not particularly limited, and the conventional method in the field can be adopted.
In the present invention, the water holding capacity (mass ratio) of the soil to be cultured is preferably 18% to 25%, more preferably 20%; the optical density of the culture is preferably 750-850 mmol/m-2·s-1More preferably 800mmol · m-2·s-1(ii) a The illumination duration is preferably 15.5-16.5 h.d-1More preferably 16 h.d-1(ii) a The culture temperature is preferably 25-27 ℃, and more preferably 26 ℃; the humidity of the culture is preferably 62% to 68%, more preferably 65%.
The technical solutions provided by the present invention are described in detail below with reference to examples, but they should not be construed as limiting the scope of the present invention.
Example 1 Toona sinensis
1. Selecting garden soil, peat and river sand according to the volume ratio of 3:1:1, sieving by a 2mm sieve, uniformly mixing, sterilizing by high-temperature (121 ℃) and high-pressure (0.1MPa) steam for 0.5h, and naturally cooling to obtain the matrix. The basic physicochemical properties of the matrix are as follows: pH value of 5.99 and organic matter content of 40.46 g/kg-1(ii) a Organic carbon: 69.75 g/kg-1(ii) a Total phosphorus: 0.75 g/kg-1(ii) a Total nitrogen: 1.90 g.kg-1(ii) a Ammonium nitrogen: 15.07 mg/kg-1(ii) a Nitrate nitrogen: 4.21 mg/kg-1。
2. Selecting full euscaphis konishii hayata seeds collected in the same year, and adding 10% of H2O2Soaking in the solution for 10min, sterilizing, and washing with sterile water2O2Soaking the residual liquid in 65 deg.C boiled water, and continuously soaking in sterile water for 5 days (changing water every day) after natural cooling. Then putting the seeds into an incubator at 25 ℃ for accelerating germination.
3. The specification of the plastic flowerpot for the test is as follows: the upper caliber is multiplied by the lower caliber and multiplied by the height is respectively 9 multiplied by 6 multiplied by 8 cm; for a total of 20 pots. 120g of matrix is filled in each pot, then 60g of microbial inoculum is inoculated, (the microbial inoculum contains 600 spores of glochidia discoidea Sv-01, Sv for short, and the preservation number is CGMCC No.18594), 3 seedlings of euscaphis konishii with consistent growth vigor are respectively planted in each pot, 60g of matrix is covered, 200mL of water is watered in each pot, and 1 seedling is fixed after the growth of the seedlings is stable.
4. Quantitatively watering every week, keeping the water holding capacity of the soil at 20% (mass ratio), and keeping the optical density at 800 mmol.m-2·s-1The light is supplemented by an agricultural sodium lamp, the illumination time is 16 hours per day, the temperature is 26 ℃, and the humidity is 65%.
Comparative example 1
The setup was the same as in example 1 except that no inoculum was inoculated. The results of comparing example 1 with comparative example 4 are shown in tables 1 to 3.
Results of the experiment
1. Influence of inoculated plasmodiophora mucilaginosa Sv-01 on growth of euscaphis konishii hayata seedlings
As can be seen from Table 4, the inoculation of glomus clathratus Sv-01 (Sv for short, example 1) can significantly promote the biomass growth of euscaphis konishii hayata seedlings compared with the control (comparative example 1), wherein the leaf number of the container seedlings (increased 274.7%), the plant height (increased 224.8%) and the ground diameter (increased 141.9%) are obviously increased.
TABLE 1 Effect of inoculation with Gloeostereum mucronatum Sv-01 on overground growth of Cedrela conifer
Figure BDA0002308439200000061
Note: the different letter representations in the same column differ significantly at the 0.05 level. (the same below)
As can be seen from Table 2, inoculation of the target strain Sv enables biomass of different organs of the euscaphis konishii hayata container seedling to be significantly increased. After Sv inoculation, the dry weight of roots of the euscaphis konishii hayata seedlings is increased by 37 times, the weight of stems is increased by 16.3 times, the dry weight of leaves is increased by 40 times, and the total dry weight is increased by 16.6 times (differences reach a very significant level, and p is less than 0.01). Proved that the inoculation indeed improves the nutrient absorption efficiency of the euscaphis konishii hayata. The root-cap ratio is an important index for representing the energy distribution of the plant, the lower the root-cap ratio is, the normal growth of the overground part of the plant can be maintained by using fewer root systems, and the higher the root-cap ratio is, the more the distribution of substances used for the root systems of the underground part of the plant to maintain the growth is proved. The root crown of the euscaphis konishii hayata after the inoculation of the target strain is 54.1 percent smaller than that of the euscaphis konishii hayata after the control treatment without inoculation, which proves that the euscaphis konishii hayata after the inoculation can maintain the biomass growth of the overground part of the euscaphis konishii hayata with less root system proportion, wherein microorganisms can play a role in assisting plants to absorb nutrients and promoting growth.
TABLE 2 Effect of inoculation with Gloeostereum mucronatum Sv-01 on Toona sinensis Biomass
Figure BDA0002308439200000062
Figure BDA0002308439200000071
2. Effect of inoculated mucosacculus isolariceus Sv-01 on photosynthetic characteristics of euscaphis konishii hayata seedlings the net photosynthetic rate means that plant photosynthesis absorbs CO2Is subtracted from respiration to produce CO2I.e. the actual photosynthetic rate of the plant. As can be seen from Table 3, inoculation of Gliocladium marcescens Sv-01 can significantly increase the net photosynthetic rate of Euscaphis konishii seedlings (increase of 122.5%, p)<0.05), promoting the photosynthesis to proceed.
The pores being CO2The gas inlet and outlet channels of the blade, and the gas hole conductance Gs represents the CO entering the surface of the blade in unit time and per unit area2The amount of (c) can reflect the degree of pore opening; intercellular CO2Concentration (Ci) is CO in mesophyll intercellular spaces inside the leaves2Concentration of external CO2The balance of photosynthesis and respiration inside the leaf and various driving forces and resistances applied to the gas entering mesophyll cells; the transpiration rate Tr is the amount of water lost by the leaves through the transpiration in a certain unit time, and can reflect the strength of the transpiration of the plants. As can be seen from Table 3, the stomatal conductance and intercellular CO of Euscaphis konishii after the inoculation of the target strain2Both the concentration and the transpiration rate increased.
TABLE 3 Effect of inoculation with Sv-01 Mucor mucosae on photosynthetic characteristics of Toona euonyssima container seedlings
Figure BDA0002308439200000072
Example 2 Toona sinensis
1. Selecting garden soil, peat and river sand according to the volume ratio of 3:1:1, sieving by a 2mm sieve, uniformly mixing, sterilizing by high-temperature (121 ℃) and high-pressure (0.1MPa) steam for 0.5h, and naturally cooling to obtain the matrix. The basic physicochemical properties of the matrix are as follows: pH value of 5.02 and organic matter content of 32.47 g/kg-1(ii) a Organic carbon: 55.98 g.kg-1(ii) a Total phosphorus: 0.509g kg-1(ii) a Total nitrogen: 1.25 g.kg-1(ii) a Total potassium: 1.79 mg/kg-1。
2. Selecting plump toona sinensis seeds collected in the same year, and diluting with 10% H2O2Soaking the solution for 5min, and removing residual H from the seeds with distilled water2O2The solution is washed and soaked in distilled water for one night for standby. Spreading the cooled sand in a tray, mixing with the soaked seeds, placing in an incubator for germination, keeping the temperature at 25 deg.C, keeping the humidity at 66%, and irradiating with sunlight for 12 hr, and irrigating the seeds with distilled water in the morning and evening every day. After 2 true leaves grow out, the seeds are embedded into sterile river sand which is sterilized at 121 ℃ for 30min and cooled, and are cultured and seedlings emerge for later use.
3. The specification of the plastic flowerpot for the test is as follows: the upper caliber is 16 multiplied by 11 multiplied by 12.5cm, the lower caliber is multiplied by the height, and the water is used after being wiped and disinfected by 70 percent alcohol, and the total number of the water is 25 basins. 300g of substrate is loaded in each pot, then 60g of microbial inoculum is inoculated (the microbial inoculum contains 600 Sv-01 spores of gloeosporium viscidum, Sv for short, and the preservation number is CGMCC No.18594), 3-4 toona sinensis seedlings with good growth vigor are respectively selected for transplanting in each pot, 120g of substrate is covered, and the seedlings are laid flat and compacted. After the nursery stock grows stably, 1 plant is fixed.
4. Quantitatively watering every week, keeping the water holding capacity of the soil at 20% (mass ratio), and keeping the optical density at 800 mmol.m-2·s-1The light is supplemented by an agricultural sodium lamp, the illumination time is 16 hours per day, the temperature is 26 ℃, and the humidity is 65%.
Comparative example 2
The setup was the same as in example 2 except that no inoculum was inoculated. The results of comparing example 2 with comparative example 2 are shown in tables 4 to 6.
Results of the experiment
1. Influence of inoculated mucosacculus isolariceus Sv-01 on growth of toona sinensis seedlings
As can be seen from table 4, inoculation with glomus mucosae (example 2) can promote toona sinensis seedling biomass growth with a significant level of seedling growth in diameter (60.1%, p <0.05) compared to the control (comparative example 2). The plant height and the leaf number of the seedlings of the toona sinensis are increased after the inoculation of the mucose sacculus isolariceus Sv-01 (8.6 percent and 1.9 percent respectively).
TABLE 4 influence of inoculation with Gliocladium viscidum Sv-01 on overground growth of young Toonae sinensis
Figure BDA0002308439200000081
Note: data in the table are mean ± standard deviation. The different lower case letters in the same column represent significant differences at the 0.05 level, as follows.
As can be seen from Table 5, the inoculated mucosaccus sepalatus also has a relatively good promoting effect on the development of the root system of the toona sinensis (Table 3), the total root length of the toona sinensis seedlings after inoculation is increased by 78.8%, the root surface area is increased by 97.8%, the root volume is increased by 98.9%, and the differences reach a significant level (p is less than 0.05). The average root diameter of the young seedlings of the toona sinensis is increased by 10.1 percent after inoculation.
TABLE 5 influence of inoculation with Gliocladium viscidum Sv-01 on development of root System of Toona sinensis seedlings
Figure BDA0002308439200000082
Figure BDA0002308439200000091
As can be seen from table 6, inoculation of mucosacculus isolariceus can significantly increase the biomass of different organs of the young toona sinensis: after inoculation, the dry weight of the root of the young Chinese toon seedlings is increased by 110.3%, the weight of the stems is increased by 116.7%, the dry weight of the leaves is increased by 82.8%, the total dry weight is increased by 97.3%, and the difference reaches a significant level (p < 0.05). The ratio of the root cap of the inoculated Chinese toon seedlings to the root cap is increased by 8.8 percent, which shows that the target AM fungus realizes the increase of the biomass of the Chinese toon through the balanced promotion of the overground and underground parts.
TABLE 6 Effect of inoculation with Gliocladium viscidum Sv-01 on Toona sinensis seedling Biomass
Figure BDA0002308439200000092
Example 3 Heracleum Macranthum
1. Selecting garden soil, peat and river sand according to the volume ratio of 3:1:1, sieving by a 2mm sieve, uniformly mixing, sterilizing by high-temperature (121 ℃) and high-pressure (0.1MPa) steam for 0.5h, and naturally cooling to obtain the matrix. The basic physicochemical properties of the matrix are as follows: pH value of 5.99 and organic matter content of 40.458 g/kg-1(ii) a Organic carbon: 69.750 g/kg-1(ii) a Total phosphorus: 0.754 g.kg-1(ii) a Total nitrogen: 1.903 g/kg-1(ii) a Ammonium nitrogen: 15.068 mg/kg-1(ii) a Nitrate nitrogen: 4.209 mg/kg-1。
2. Selecting the full Cinnamomum zeylanicum seeds collected in the same year, and adding 10% H2O2Soaking in the solution for 10min, sterilizing, and washing with sterile water2O2And (4) putting the residual liquid into a 4 ℃ thermostat, storing at low temperature for 1 month, taking out, embedding into sterile river sand sterilized at 121 ℃ for 30min, and culturing to obtain seedlings for later use.
3. The specification of the plastic flowerpot for the test is as follows: the upper caliber is multiplied by the lower caliber and multiplied by the height is respectively 9 multiplied by 6 multiplied by 8 cm; for a total of 20 pots. 180g of matrix is filled in each pot, then 60g of microbial inoculum (the microbial inoculum contains 600 spores of plasmopara saccularis Sv-01, Sv for short, and the preservation number is CGMCC No.18594), 3 seedlings of the germinated cinnamomum zeylanicum are planted, 60g of matrix is covered, 200mL of water is added to each pot, and 1 seedling is fixed after the growth of the seedlings is stable.
4. Quantitatively watering every week, keeping the water holding capacity of the soil at 20% (mass ratio), and keeping the optical density at 800 mmol.m-2·s-1By agricultureAnd (3) supplementing light by a sodium lamp, wherein the illumination time is 16h per day, the temperature is 26 ℃, and the humidity is 65%. Weighing once every three days, recording the growth conditions (plant height, ground diameter and leaf number) of the overground part of the cinnamomum kanahirai hance, observing the root development conditions (total root length, root surface area, root volume, average root diameter and root tip number) of the cinnamomum kanahirai hance, and counting the biomass (root dry weight, stem dry weight, leaf dry weight, total dry weight and root crown ratio) of the cinnamomum kanahirai hance. The results are shown in tables 7 to 9
Comparative example 3
The setup was the same as in example 3 except that no inoculum was inoculated. See tables 7-9 for results.
TABLE 7 Effect of inoculation with Sv-01 of Gliocladium viscidum on overground growth of Cinnamomum zeylanicum
Figure BDA0002308439200000101
Note: data in the table are mean ± standard deviation. Different lower case letters in the same column represent significant differences at the 0.05 level and different upper case letters represent significant differences at the 0.01 level. The same applies below.
As can be seen from Table 7, inoculation of Gliocladium marcescens (example 3) significantly promoted biomass growth of Cinnamomum zeylanicum seedlings with a significant increase in leaf number, plant height and ground diameter as compared to control (comparative example 3).
TABLE 8 influence of inoculation of Gliocladium viscidum Sv-01 on root development of Cinnamomum zeylanicum
Figure BDA0002308439200000102
As can be seen from Table 8, the inoculation of the mucoid sacculus isolaricires Sv-01 also has a relatively good promoting effect on the development of the root system of the cinnamomum kanahirai hance, and the inoculation Sv has a significant growth promoting effect on the total root length, the root surface area, the root volume and the number of root tips of the cinnamomum kanahirai hance seedlings as can be seen from Table 3, wherein the total root length of the cinnamomum kanahirai hance seedlings after inoculation is increased by 30.1%, the root surface area is increased by 41.3%, the root volume is increased by 61.5%, and the number of the root tips is increased by 54.3% (the difference reaches a significant level, and p is less than 0.. The average root diameter of the cinnamomum longepaniculatum after inoculation is increased by 12.3 percent.
TABLE 9 Effect of Gliocladium viscidum Sv-01 inoculation on Cinnamomum zeylanicum Biomass
Figure BDA0002308439200000103
As can be seen from Table 4, the inoculation of the Sphaerotheca viscida Sv-01 can obviously improve the biomass of different organs of the Cinnamomum kanehirai Hayata container seedling, wherein the dry weight of the roots of the Cinnamomum kanehirai Hayata is increased by 135.1% (p < 0.05); shoot weight increased 194.7% (p < 0.05); leaf dry weight increase 353.3% (p < 0.05); the total dry weight increase was 211.3% (p < 0.05). And the root-crown ratio of the cinnamomum kanahirai dunn seedlings treated by inoculating Sv is 0.59, which is 37.2% smaller than that treated by non-inoculating bacteria (p is less than 0.05), which shows that the cinnamomum kanahirai dunn seedlings maintain more overground part growth with less underground root system amount and have higher nutrient absorption and utilization efficiency.
The above examples show that the inoculation of the glomus viscidus Sv-01 has a very good promoting effect on the growth of the container seedlings of the tonka, the euscaphis konishii hayata or the toona sinensis.
The foregoing is only a preferred embodiment of the present invention, and it should be noted that, for those skilled in the art, various modifications and decorations can be made without departing from the principle of the present invention, and these modifications and decorations should also be regarded as the protection scope of the present invention.
Sequence listing
<110> university of agriculture in Jiangxi
<120> Gloeostereum viscidum, microbial inoculum comprising Gloeostereum viscidum and application
<160> 1
<170> SIPOSequenceListing 1.0
<210> 1
<211> 539
<212> DNA
<213> Claroidomus claroides (Claroiidoglomus claroideum)
<400> 1
gggacctcat cagctcatag cgtatattaa agttgttgca gttaaaaagc tcgtagttga 60
acttcgggat caatggattg gtcatgccga tggtgtgtac tggtcatatt ggttctcacc 120
ttctaaggaa ccataatgtc atttatttga tgttgtgggg aattaggact gttaccttga 180
aaaaattaga gtgtttaaag caggctaacg cttgaataca ttagcatgga ataatgaaat 240
aggacatcgc attctatttt gttggtttct aggatcgatg taatgattaa tagggatagt 300
tgggggcatt agtattcaat tgtcagaggt gaaattcttg gatttattga agactaacta 360
ctgcgaaagc atttgccaag gatgttttca ttaatcaaga acgaaagtta ggggatcgaa 420
gacgatcaga taccgtcgta gtcttaacca taaactatgc cgactaggga tcggatgatg 480
ttgatttttt aatgactcat tcggcacctt acgggaaacc aaagtgtttg ggttcaata 539

Claims (8)

1. A strain of glomus viscus Sv-01 with the preservation number of CGMCC No. 18594.
2. An agent comprising the mucosepalax of claim 1.
3. The microbial inoculum according to claim 2, wherein the effective spore number of the gloeosporium mucosum in the microbial inoculum is 10-80/g.
4. Use of the glomus mucosae of claim 1 or the microbial inoculum of claim 2 or 3 for promoting growth of seedlings in plant containers, promoting photosynthesis and/or promoting transpiration of plants, comprising the steps of: filling a substrate and a microbial inoculum in a container from bottom to top in sequence, planting plant seedlings, then covering the substrate, watering, fixing seedlings after the plant seedlings grow stably, and culturing; the plant is euscaphis konishii hayata, cinnamomum longepaniculatum or toona sinensis.
5. The use according to claim 4, wherein the matrix comprises the following parts by volume of raw materials: 2.8-3.2 parts of garden soil, 0.8-1.2 parts of grass carbon and 0.8-1.2 parts of river sand.
6. The use of claim 4, wherein the container comprises a plastic pot.
7. The use according to claim 6, wherein the number of seedlings per container is 1-2.
8. The use according to claim 4, wherein the optical density of the culture is 750 to 850 mmol-m-2·s-1The illumination time is 15.5-16.5 h.d-1The temperature is 25-27 ℃, and the humidity is 62-68%.
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Citations (1)

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CN110249989A (en) * 2019-07-22 2019-09-20 云南省林业科学院 A kind of olive mycorrhiza fungi seeding cultivating method

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Publication number Priority date Publication date Assignee Title
CN110249989A (en) * 2019-07-22 2019-09-20 云南省林业科学院 A kind of olive mycorrhiza fungi seeding cultivating method

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* Cited by examiner, † Cited by third party
Title
"AM fungi facilitate the competitive growth of two invasive plant species, Ambrosia artemisiifolia and Bidens pilosa";Fengjuan Zhang, et al.;《Mycorrhiza》;20180915;第28卷(第8期);全文 *
"Impact of beneficial microorganisms on strawberry growth, fruit production, nutritional quality, and volatilome";Valeria Todeschini, et al.;《Front Plant Sci》;20181116;第9卷;第3页左栏第2段、右栏第3段，第4页左栏第2段，第5页右栏第2、4、5段，第7页表2，第8页图2，第16页表7；第2页左栏第1段，第18页右栏第2段 *
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