CN110806489A - Anti-interference dry chemical joint detection device - Google Patents
Anti-interference dry chemical joint detection device Download PDFInfo
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- CN110806489A CN110806489A CN201910977501.8A CN201910977501A CN110806489A CN 110806489 A CN110806489 A CN 110806489A CN 201910977501 A CN201910977501 A CN 201910977501A CN 110806489 A CN110806489 A CN 110806489A
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- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
- G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
- G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
- G01N33/72—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing involving blood pigments, e.g. haemoglobin, bilirubin or other porphyrins; involving occult blood
- G01N33/721—Haemoglobin
- G01N33/726—Devices
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- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
- G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
- G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
- G01N33/53—Immunoassay; Biospecific binding assay; Materials therefor
- G01N33/573—Immunoassay; Biospecific binding assay; Materials therefor for enzymes or isoenzymes
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- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N2333/00—Assays involving biological materials from specific organisms or of a specific nature
- G01N2333/90—Enzymes; Proenzymes
- G01N2333/91—Transferases (2.)
- G01N2333/91188—Transferases (2.) transferring nitrogenous groups (2.6)
Abstract
The invention discloses an anti-interference dry chemical combined detection device which comprises a base, a sample adding cover, a flow distribution layer, a separation block, a first detection channel and a second detection channel, wherein the separation block is arranged in the middle of one side of the base, the first detection channel and the second detection channel are respectively arranged on two sides of the separation block, a first non-return layer is arranged in the first detection channel, a second non-return layer is arranged in the second detection channel, the flow distribution layer and the sample adding cover are sequentially arranged above the separation block, the flow distribution layer covers the first detection channel and the second detection channel, and a sample adding hole is formed in the sample adding cover. The anti-interference dry chemical combined detection device is simple in structure and easy to manufacture, the arrangement of the flow dividing layer, the first non-return layer and the second non-return layer can effectively ensure that blood flows from the sample adding hole to the first detection channel and the second detection channel respectively, cross contamination or mutual interference caused by backflow can not be caused, and two functional detections can be performed simultaneously by one-time blood adding.
Description
Technical Field
The invention relates to an anti-interference dry chemical combined detection device.
Background
In clinical examination analysis, hemoglobin and glutamic-pyruvic transaminase are important physical examination items, especially student physical examination, and the two items are essential physical examination items. However, two items of hemoglobin and glutamic-pyruvic transaminase belong to different test categories, hemoglobin is a blood routine test item and is generally used for quantitatively detecting a whole blood sample by using a blood analyzer and a matched solution, while glutamic-pyruvic transaminase is a liver function test item and is a serum biochemical item and is generally used for quantitatively detecting a serum sample by using a full-automatic biochemical analyzer and a matched ALT kit. When the sample is collected, different blood collection tubes are required to be used for collecting and processing blood respectively, and different analysis systems are used for completing the detection of two items respectively. The operation is relatively cumbersome.
The application of the dry chemical method can facilitate the detection work to a great extent, a plurality of serum biochemical projects can directly use a whole blood sample for detection, and a plurality of test paper for combined detection are on the market. The market can see a dry chemical quantitative analysis instrument for hemoglobin or glutamic-pyruvic transaminase and different detection test papers aiming at a single item respectively, even if the so-called hemoglobin and glutamic-pyruvic transaminase combined detection test paper exists, the hemoglobin test paper and the glutamic-pyruvic transaminase test paper are simply arranged and are relatively independent of each other, each detection item needs to be added with a blood sample respectively and independently, and the detection is not a convenient combined quick detection product of 'one drop of blood one step detection' in the true sense.
However, no report is found on a dry chemical test paper product which can simultaneously carry out quantitative detection on two items of hemoglobin and glutamic-pyruvic transaminase by one drop of blood and one sample adding. The reason is that the hemoglobin detection needs the participation of hemolytic agent, and the hemolytic reagent can cause serious interference to the detection system for detecting the glutamic pyruvic transaminase content in the serum (sample hemolysis can interfere with the detection of serum glutamic pyruvic transaminase), so that the integration of the hemoglobin and the detection unit of the glutamic pyruvic transaminase and the detection unit of the hemoglobin to realize the synchronous quantitative detection of one-drop blood sample adding is a worldwide problem in the detection field.
Therefore, a new dry chemical combination detection device of hemoglobin and glutamic-pyruvic transaminase resistant to mutual interference is needed to solve the above problems.
Disclosure of Invention
The invention aims to provide an anti-interference dry chemical combined detection device, which solves the problem that quantitative detection of only one item can be completed by one drop of blood sample adding in the background technology.
In order to achieve the purpose, the invention provides the following technical scheme:
an anti-interference dry chemical combined detection device comprises a base, a sample adding cover, a flow dividing layer, a separating block, a first detection channel and a second detection channel, wherein the separating block is arranged in the middle of one side of the base, the first detection channel and the second detection channel are respectively arranged on two sides of the separating block, a first non-return layer is arranged in the first detection channel, a second non-return layer is arranged in the second detection channel, the flow dividing layer and the sample adding cover are sequentially arranged above the separating block, the flow dividing layer covers the first detection channel and the second detection channel, and a sample adding hole is formed in the sample adding cover;
the shunting layer is formed by mutually and vertically crossing and weaving first thick lines and first thin lines, the first thick lines are all warps, the first thin lines are all wefts, and the first thick lines cross the first detection channel and the second detection channel;
the first non-return layer is formed by mutually and vertically crossing and weaving second thick lines and second thin lines, the second thick lines are all warps, and the second thin lines are all wefts;
the second non-return layer is formed by vertically and crosswise weaving third thick lines and third thin lines, the third thick lines are all warp yarns, and the third thin lines are all weft yarns;
the second thick line and the third thick line are arranged in a crossed manner with the first thick line.
Furthermore, the upper surface of the dividing pad is higher than the first non-return layer and the second non-return layer.
Furthermore, a blood filtering membrane is arranged in the first detection channel and is arranged below the first non-return layer.
Furthermore, a first flow pad is arranged in the first detection channel, one end of the first flow pad is arranged below the blood filtering membrane, and the other end of the first flow pad extends out of the first detection channel.
Furthermore, the reaction device further comprises a first reaction panel, wherein the first reaction panel is positioned right above the first flow pad, a first reaction through hole is formed in the first reaction panel, and a first reaction film is attached to the lower portion of the first reaction through hole.
Furthermore, a sample pad is arranged in the second detection channel, one end of the sample pad is arranged below the second non-return layer, and the other end of the sample pad extends out of the second detection channel.
Furthermore, still be provided with the second reaction panel in the second detection channel, the second reaction panel sets up directly over the sample pad, be provided with the second reaction through-hole on the second reaction panel, the below of second reaction through-hole is pasted and is had the second reaction membrane.
Furthermore, a clamping groove is formed in the separation block, a protrusion is arranged on the opposite surface of the sample adding cover and the separation block, and the protrusion is in interference fit with the clamping groove.
Furthermore, the flow distribution layer, the first non-return layer and/or the second non-return layer are monofilament mesh cloth.
Furthermore, the second thick line and the third thick line are arranged perpendicularly across the first thick line.
Further, the first thick line, the first thin line, the second thick line, the second thin line, the third thick line and the third thin line are all hydrophilic materials.
Further, the hydrophilic material is polyester or nylon.
Further, the first thick line has a diameter 2 to 4 times the diameter of the first thin line, the second thick line has a diameter 2 to 4 times the diameter of the second thin line, and the third thick line has a diameter 2 to 4 times the diameter of the third thin line.
Furthermore, the first thick lines are all wefts, and the first thin lines are all warps; the second thick lines are all wefts, and the second thin lines are all warps; the third thick lines are all wefts, and the third thin lines are all warps.
Has the advantages that: the anti-interference dry chemical combined detection device is simple in structure and easy to manufacture, the arrangement of the flow dividing layer, the first non-return layer and the second non-return layer can effectively ensure that blood flows from the sample adding hole to the first detection channel and the second detection channel respectively, cross contamination or mutual interference caused by backflow can not be caused, and two functional detections can be performed simultaneously by one-time blood adding.
Drawings
FIG. 1 is a schematic structural diagram of an anti-interference dry chemical combination detection device of the present invention;
FIG. 2 is a left side view of the interference resistant dry chemical combination detection device of the present invention;
FIG. 3 is a right side view of the interference resistant dry chemical combination detection device of the present invention.
Detailed Description
The technical solutions in the embodiments of the present invention will be clearly and completely described below with reference to the drawings in the embodiments of the present invention, and it is obvious that the described embodiments are only a part of the embodiments of the present invention, and not all of the embodiments. All other embodiments, which can be derived by a person skilled in the art from the embodiments given herein without making any creative effort, shall fall within the protection scope of the present invention.
Referring to fig. 1-3, the anti-interference dry chemical combined detection device of the present invention includes a base 1, a sample application cover 2, a flow distribution layer 3, a separation block 4, a first detection channel and a second detection channel, wherein the separation block 4 is disposed in the middle of one side of the base 1, the first detection channel and the second detection channel are disposed on two sides of the separation block 4 respectively, a first non-return layer 5 is disposed in the first detection channel, a second non-return layer 6 is disposed in the second detection channel, the flow distribution layer 3 and the sample application cover 2 are sequentially disposed above the separation block 4, the flow distribution layer 3 covers the first detection channel and the second detection channel, and a sample application hole 21 is disposed on the sample application cover 2.
The shunting layer 3 is formed by mutually and vertically and crosswise weaving first thick lines and first thin lines, the first thick lines are all warps, the first thin lines are all wefts, and the first thick lines cross the first detection channel and the second detection channel.
The first non-return layer 5 is formed by mutually and vertically and crossly weaving second thick lines and second thin lines, wherein the second thick lines are all warps, and the second thin lines are all wefts.
The second non-return layer 6 is formed by mutually and vertically crossing and weaving third thick lines and third thin lines, wherein the third thick lines are all warps, and the third thin lines are all wefts.
The second thick line and the third thick line are arranged to intersect with the first thick line.
Preferably, the upper surface of the division pad 4 is higher than the first and second backstop layers 5 and 6. The blood on the first non-return layer 5 and the second non-return layer 6 can be effectively prevented from flowing back to the shunting layer 3.
Preferably, a blood filter 51 is further disposed in the first detection channel, and the blood filter 51 is disposed below the first non-return layer 5. The blood filter 51 can filter the red blood cells in the blood, and is convenient for glutamic-pyruvic transaminase detection.
Preferably, a first flow pad 52 is further disposed in the first detection channel, one end of the first flow pad 52 is disposed below the blood filtration membrane 51, and the other end of the first flow pad 52 extends out of the first detection channel. The blood from which the red blood cells have been filtered is drained to the glutamic pyruvic transaminase detection reaction panel by the first flow pad 52.
Preferably, the reaction device further comprises a first reaction panel 53, wherein the first reaction panel 53 is located right above the first flow pad 52, and the first reaction panel 53 is provided with a first reaction through hole.
Preferably, a sample pad 61 is further disposed in the second detection channel, one end of the sample pad 61 is disposed below the second non-return layer 6, and the other end of the sample pad 61 extends out of the second detection channel. Blood is drained to the hemoglobin detection reaction panel using the sample pad 61.
Preferably, a second reaction panel 62 is further disposed in the second detection channel, the second reaction panel 62 is disposed right above the sample pad 61, and a second reaction through hole is disposed on the second reaction panel 62.
Preferably, the separating block 4 is provided with a clamping groove, and the opposite surface of the sample adding cover 2 and the separating block 4 is provided with a bulge which is in interference fit with the clamping groove. The cooperation of application of sample lid and cutting apart pad is realized to utilization interference fit, convenient and fast.
Preferably, the diversion layer 2, the first non-return layer 5 and/or the second non-return layer 6 are monofilament fabric. The shunting and the countercurrent of the blood are conveniently realized.
Preferably, the second thick line and the third thick line are arranged perpendicularly across the first thick line. The second thick line and the third thick line are vertically crossed with the first thick line, so that the blood non-return effect can be realized to the maximum extent on the basis of conveniently realizing shunting.
Preferably, the first thick line, the first thin line, the second thick line, the second thin line, the third thick line, and the third thin line are all hydrophilic materials. The hydrophilic material can realize the blood non-return effect to the maximum extent on the basis of conveniently realizing shunt.
Preferably, the hydrophilic material is polyester or nylon.
Preferably, the first thick line has a diameter 2 to 4 times the diameter of the first thin line, the second thick line has a diameter 2 to 4 times the diameter of the second thin line, and the third thick line has a diameter 2 to 4 times the diameter of the third thin line.
Preferably, the first thick lines are all wefts, and the first thin lines are all warps. The second thick lines are all wefts, and the second thin lines are all warps. The third thick lines are all wefts, and the third thin lines are all warps.
Example 1:
the anti-interference dry chemical joint detection device is used for realizing dry chemical joint detection of hemoglobin and glutamic-pyruvic transaminase and comprises a base, an upper cover, a reaction window, a sample adding hole cover and a sample processing unit.
The sample processing unit comprises a shunting layer, two independent non-return layers separated from each other, a sample pad for hemoglobin detection, and a blood filtering membrane and a flow pad for glutamic-pyruvic transaminase detection.
The sample processing unit comprises a shunting layer covering the two detection channels and is used for shunting the blood sample to the hemoglobin detection channel and the glutamic-pyruvic transaminase detection channel.
The shunt layer can be a hydrophilic mesh material, such as a mesh of polyester, nylon, or the like. The single-wire mesh cloth with larger diameter difference between the warp and the weft can be used, and the two detection channels are transversely arranged in the thick line direction.
The sample processing unit comprises two independent and mutually separated non-return layers which are respectively arranged in the hemoglobin detection channel and the glutamic-pyruvic transaminase detection channel and used for preventing the blood sample from being reversely transmitted back to the shunting layer and causing the chemical reagent of the hemoglobin detection channel to interfere the normal detection of the glutamic-pyruvic transaminase detection channel through the bridging action of the shunting layer.
The non-return layer can be a hydrophilic mesh material, such as mesh (filter cloth) made of polyester, nylon and the like. The single-wire mesh cloth with larger diameter difference between the warps and the wefts can be used, the thick wire direction is longitudinally arranged and is mutually perpendicular and crossed with the thick wire direction of the shunting layer, so that the contact area between the shunting layer and the non-return layer is reduced, and the non-return effect is achieved.
The reaction window comprises a hemoglobin detection window and a glutamic-pyruvic transaminase reaction window, a reaction membrane is adhered on the reaction window, and the reaction window is connected with the upper cover through interference fit; the upper cover is provided with a plurality of small columns which are in interference fit with the corresponding grooves on the base, and the upper cover and the base can be fixed together in a pressing mode; the middle of the sample adding hole cover is provided with a round hole for adding a blood sample, the periphery of the sample adding hole cover is provided with a plurality of small columns, the small columns are in interference fit with corresponding grooves on the base, and the sample adding hole cover and the base are fixed together in a pressing mode.
The reaction window comprises a hemoglobin detection window and a glutamic-pyruvic transaminase reaction window, a reaction membrane is adhered on the reaction window, and a reaction reagent participating in the color development reaction of the hemoglobin or the glutamic-pyruvic transaminase is solidified on the reaction membrane and is distributed in the reaction membrane network structure in a solid state. The reaction membrane can be a nitrocellulose membrane, a nylon membrane, a polyester membrane, a polyether sulfone membrane and other materials.
The area of the reaction membrane is slightly larger than that of the window so as to be convenient for being adhered to the colloid near the window, the reaction membrane is positioned right above the sample pad (or the flow pad) and keeps a non-contact state with the sample pad (or the flow pad), but partial overlapped areas are required in the vertical direction so as to be beneficial to realizing mutual contact under the joint control of a matched analysis instrument, so that the blood (or serum) sample on the sample pad (or the flow pad) is transferred to the reaction membrane, and the color reaction is finished.
The sample pad is solidified with hemolysis reagent, reaction reagent and auxiliary reagent which participate in hemoglobin color reaction, and the hemolysis reagent, the reaction reagent and the auxiliary reagent are distributed in the sample pad mesh structure in a solid state. The sample pad may be fiberglass, cotton, polyester, or the like.
The anti-interference dry chemical combined detection device is simple in structure and easy to manufacture, the arrangement of the flow dividing layer, the first non-return layer and the second non-return layer can effectively ensure that blood flows from the sample adding hole to the first detection channel and the second detection channel respectively, cross contamination or mutual interference caused by backflow can not be caused, and two functional detections can be performed simultaneously by one-time blood adding.
Claims (14)
1. An anti-interference dry chemical combined detection device comprises a base (1), a sample adding cover (2), a shunting layer (3), a separation block (4), a first detection channel and a second detection channel, wherein the separation block (4) is arranged in the middle of one side of the base (1), the first detection channel and the second detection channel are respectively arranged on two sides of the separation block (4), a first non-return layer (5) is arranged in the first detection channel, a second non-return layer (6) is arranged in the second detection channel, the shunting layer (3) and the sample adding cover (2) are sequentially arranged above the separation block (4), the shunting layer (3) covers the first detection channel and the second detection channel, and a sample adding hole (21) is formed in the sample adding cover (2);
the shunting layer (3) is formed by mutually and vertically crossing and weaving first thick lines and first thin lines, the first thick lines are all warps, the first thin lines are all wefts, and the first thick lines cross the first detection channel and the second detection channel;
the first non-return layer (5) is formed by mutually and vertically crossing and weaving second thick lines and second thin lines, the second thick lines are all warp yarns, and the second thin lines are all weft yarns;
the second non-return layer (6) is formed by vertically and crosswise weaving third thick lines and third thin lines, the third thick lines are all warp yarns, and the third thin lines are all weft yarns;
the second thick line and the third thick line are arranged in a crossed manner with the first thick line.
2. The interference-free, dry-chemical combination detection device of claim 1, wherein: the upper surface of the dividing pad (4) is higher than the first non-return layer (5) and the second non-return layer (6).
3. The interference-free, dry-chemical combination detection device of claim 1, wherein: a blood filtering membrane (51) is further arranged in the first detection channel, and the blood filtering membrane (51) is arranged below the first non-return layer (5).
4. The interference-free, dry-chemical combination detection device of claim 3, wherein: still be provided with first flow pad (52) in the first detection passageway, the one end setting of first flow pad (52) is in the below of blood filtration membrane (51), the other end of first flow pad (52) stretches out first detection passageway.
5. The interference-free, dry-chemical combination detection device of claim 4, wherein: still include first reaction panel (53), first reaction panel (53) are located directly over first flow pad (52), be provided with first reaction through-hole on first reaction panel (53), first reaction membrane is pasted to the below of first reaction through-hole.
6. The interference-free, dry-chemical combination detection device of claim 1, wherein: and a sample pad (61) is further arranged in the second detection channel, one end of the sample pad (61) is arranged below the second non-return layer (6), and the other end of the sample pad (61) extends out of the second detection channel.
7. The interference-free, dry-chemical combination detection device of claim 6, wherein: still be provided with second reaction panel (62) in the second detection channel, second reaction panel (62) set up directly over sample pad (61), be provided with second reaction through-hole on second reaction panel (62), the below of second reaction through-hole is pasted and is had the second reaction membrane.
8. The interference-free, dry-chemical combination detection device of claim 1, wherein: the separation block (4) is provided with a clamping groove, the opposite surface of the sample adding cover (2) and the separation block (4) is provided with a bulge, and the bulge is in interference fit with the clamping groove.
9. The interference-free, dry-chemical combination detection device of claim 1, wherein: the flow dividing layer (2), the first non-return layer (5) and/or the second non-return layer (6) are made of single-wire mesh cloth.
10. The interference-free, dry-chemical combination detection device of claim 1, wherein: the second thick line and the third thick line are arranged perpendicularly to the first thick line in a crossed manner.
11. The interference-free, dry-chemical combination detection device of claim 1, wherein: the first thick lines, the first thin lines, the second thick lines, the second thin lines, the third thick lines and the third thin lines are all hydrophilic materials.
12. The interference-free, dry-chemical combination detection device of claim 11, wherein: the hydrophilic material is polyester or nylon.
13. The interference-free, dry-chemical combination detection device of claim 1, wherein: the first thick line has a diameter 2-4 times the diameter of the first thin line, the second thick line has a diameter 2-4 times the diameter of the second thin line, and the third thick line has a diameter 2-4 times the diameter of the third thin line.
14. The interference-free, dry-chemical combination detection device of claim 1, wherein: the first thick lines are all wefts, and the first thin lines are all warps; the second thick lines are all wefts, and the second thin lines are all warps; the third thick lines are all wefts, and the third thin lines are all warps.
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