CN110806382A - Integrated liquid and solid detection pool device and fluorescence detector - Google Patents
Integrated liquid and solid detection pool device and fluorescence detector Download PDFInfo
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- CN110806382A CN110806382A CN201911154307.6A CN201911154307A CN110806382A CN 110806382 A CN110806382 A CN 110806382A CN 201911154307 A CN201911154307 A CN 201911154307A CN 110806382 A CN110806382 A CN 110806382A
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- G01N21/62—Systems in which the material investigated is excited whereby it emits light or causes a change in wavelength of the incident light
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Abstract
The invention provides an integrated liquid and solid detection pool device and a fluorescence detector, wherein the detection pool device is provided with an outer shielding module, an inner shielding module, a detection pool module and a detector module, an inner shielding main body is nested in the outer shielding main body to form an integrated structure, the detection pool main body is clamped in a through hole at the bottom in the inner shielding main body, and a conical part of the detector main body is matched with a lower conical hole at the lower part of the detection pool main body and an upper conical hole of an upper cover of the inner shielding. The device is flexible and convenient to use, and the detector module can be directly inserted into an upper conical hole of the upper inner shielding upper cover and also can be directly inserted into a lower conical hole of the lower detection cell module; the detector module is locked by adopting shape matching guide and magnetic attraction, so that the automatic calibration of the light path is realized, the operation is simple and convenient, and the operation can be flexibly performed by non-professional operators; the invention adopts a two-stage shielding structure, and reduces the influence of external light on the detection result as much as possible.
Description
Technical Field
The invention belongs to the technical field of fluorescence detection, relates to a detection device for harmful substances in liquid and solid, and particularly relates to an integrated liquid and solid detection pool device and a fluorescence detector.
Background
The fluorescence detection has extremely high sensitivity and strong selectivity, and is extremely widely applied to trace and trace sample analysis. The detection cell is one of the core components of the fluorescence detector, and the design of the detection cell directly influences the performance of the whole detection system.
At present, fluorescence detectors appearing in the market are expensive, complex in operation, time-consuming, labor-consuming, even professional skills are required, and ordinary consumers are difficult to popularize and apply. Along with the great abundance of people's material life, people's health consciousness is getting stronger and stronger, pay more and more attention to the safety problems in the fields of food, environment, articles for daily use and the like, for example, the problem of vegetable pesticide residue, the problem of air quality, the problem of food additives and the like. The detection of harmful substances in liquid samples such as water quality, cosmetics, honey, fruit juice, milk and the like or solid samples such as dried fruits, preserved fruits, flour and the like usually needs to be detected by a special identification mechanism or a laboratory, but no small and cheap instrument suitable for being used by common consumers exists, and the content of the harmful substances in daily necessities of everybody can be simply, quickly and conveniently detected.
In the existing fluorescence detector, the detection cell is usually in a relatively open state, and large background noise is caused to a light path due to the refraction and reflection phenomena of light; or the detector in the fluorescence detector needs to recalibrate the light path every time the detector is disassembled and replaced, and the method is usually carried out by professional staff, so that the complexity is complex, the popularization and the application of the fluorescence detector are influenced, and the application cost is increased.
Disclosure of Invention
In order to solve the technical problems, the invention provides an integrated liquid and solid detection pool device which is compact in structure, simple in process, low in cost and stable in performance.
The invention adopts the following technical scheme:
an integrated liquid and solid detection cell device, comprising:
the shielding module comprises a shielding body, wherein the shielding body is provided with a cavity, the bottom of the shielding body is provided with a through hole (212), and the top of the shielding body is provided with an upper tapered hole (222) communicated with the cavity;
the detection cell module (3) comprises a detection cell main body (31), wherein the upper part of the detection cell main body is provided with a containing cavity (311) for placing a sample container (7), the lower part of the detection cell main body is provided with a lower tapered hole (312) communicated with the containing cavity, the containing cavity (311) of the detection cell main body (31) is communicated with the upper tapered hole (222) of the shielding body, the detection cell main body (31) is clamped at a through hole at the bottom in the shielding body, and the lower tapered hole (312) of the lower part of the detection cell main body extends out of the through hole (212); and
the detector module (4) is provided with a detector main body (41), the upper end of the detector main body (41) is provided with a conical part (411), and the conical part (411) is matched with a lower conical hole (312) at the lower part of the detection cell main body (31) and an upper conical hole (222) of the shielding body.
In the integrated liquid and solid detection pool device, the conical part (411) of the detector module (4) is arranged in the lower conical hole (312) of the detection pool main body (31), the shielding module is provided with a shielding upper cover, and the shielding upper cover covers the upper conical hole (222) of the shielding body; the detection cell module (3) further comprises a first magnetic ring (32) arranged on the periphery of the detection cell main body, a second magnetic ring (44) attracted with the first magnetic ring is arranged on the periphery of the detector main body (41), and the second magnetic ring (44) is attracted with the first magnetic ring (32) to fix the conical part (411) of the detector main body (41) in the lower conical hole (312) of the detection cell main body (31).
In the integrated liquid and solid detection cell device, the conical part (411) of the detector module (4) is arranged at the upper conical hole (222) of the shielding body.
In the integrated liquid and solid detection pool device, the shielding module is provided with an outer shielding module (1) and an inner shielding module (2), the outer shielding module (1) comprises an outer shielding main body (11) and an outer shielding upper cover (12) hinged with the outer shielding main body, and the outer shielding main body (11) is of a hollow structure; interior shielding module (2) include interior shielding main part (21) and with interior shielding main part articulated interior shielding upper cover (22), interior shielding main part (21) nestification forms the integral type structure in outer shielding main part (11), shielding main part's bottom including through-hole (212) set up, shielding upper cover (22) including last toper hole (222) set up.
In the integrated liquid and solid detection pool device, the outer shielding upper cover (12) is hinged with the outer shielding main body (11) through a first connecting shaft (13), a buckle (121) is arranged at the edge of the outer shielding upper cover (12), and the buckle is matched with a clamp spring structure (111) arranged on the outer shielding main body (121) for use so as to buckle the outer shielding upper cover (12) at the upper opening of the outer shielding main body (11); the clamp spring structure (111) comprises a clamp spring with a hook at the upper part and a key connected with the clamp spring, and a spring is arranged between the inner side of the clamp spring and the outer shielding main body (11).
In the integrated liquid and solid detection pool device, the inner shielding main body (21) is a cylinder with an upper opening, the lower end of the outer wall of the inner shielding main body is provided with an outer step (213) which is matched and attached with an inner step (112) arranged on the inner wall of the outer shielding main body (11); an annular bulge (221) is arranged on the inner side of the inner shielding upper cover (22) and is matched and embedded with an annular groove (211) arranged on the inner shielding main body (21) when the inner shielding upper cover (22) is buckled with the inner shielding main body (21).
In the integrated liquid and solid detection cell device, the detector module (4) further comprises an induction device (42), an optical filter (43) and a light source (45) which are arranged on the detector main body (41), the detector main body (41) is provided with a cavity with a lower opening and a light source channel (413) communicated with the cavity, the light source (45) is arranged in the light source channel (413), and light emitted by the light source (45) is transmitted out through a window (412) arranged at the top of the conical part (411) of the detector main body; the cavity opening of the detector main body (41) is sequentially provided with a sensing device (42) and an optical filter (43) from outside to inside, and the optical filter (43) only allows light with relevant wavelengths to pass through.
In the integrated liquid and solid detection cell device, the outer shield, the detection cell main body (31) and the detector main body (41) are all made of black plastic materials or blackened aluminum alloy materials.
The invention also provides a fluorescence detector which comprises a microprocessor system (5) and a detection pool device (01) electrically connected with the microprocessor system (5), wherein the detection pool device (01) is the integrated liquid and solid detection pool device.
The fluorescence detector also comprises a display screen (6), and the display screen (6), a light source (45) and a sensing device (42) in the detection cell device (01) are electrically connected to the microprocessor system (5); the sample container (7) placed in the detection cell main body (31) of the detection cell device (01) is a disposable micro light-transmitting plastic cup.
By adopting the scheme, the invention has the following characteristics:
1) the device adopts an integrated structure, is flexible and convenient to use, and the detector module can be directly inserted into the upper conical hole of the upper shield body and also can be directly inserted into the lower conical hole of the lower detection cell module, so that the device is suitable for detecting liquid samples and solid or powder samples;
2) the invention adopts a two-stage shielding structure of the inner shielding module and the outer shielding module, thereby reducing the influence of external light on the detection result as much as possible;
3) the detector module is locked by adopting shape matching guide and magnetic attraction, so that the automatic calibration of the light path is realized, the operation is simple and convenient, and the operation can be flexibly performed by non-professional operators;
4) the detection cell device can be used for detecting liquid, solid or powder samples, the detection effect can be realized by adopting less samples and reagents, the reagent consumption is reduced, and the cost is saved; the sample container adopts a disposable transparent plastic cup, is convenient to use and has no secondary pollution.
Drawings
FIG. 1 is a schematic structural diagram of a fluorescence detector according to a first embodiment of the present invention;
FIG. 2 is a schematic structural diagram of a second embodiment of the fluorescence detector of the present invention;
FIG. 3 is a schematic structural diagram of an external shield module of the detection cell arrangement;
FIG. 4 is a schematic structural view of an inner shield module of the detection cell device;
FIG. 5 is a schematic view of the structure of a detection cell module of the detection cell device;
FIG. 6 is a schematic diagram of the structure of a detector module of the detection cell arrangement;
FIG. 7 is a schematic diagram of a fluorescence detector.
The reference numbers in the figures denote:
01-a detection cell arrangement;
1-an outer shielding module, 11-an outer shielding main body, 111-a clamp spring structure, 112-an inner step, 12-an outer shielding upper cover, 121-a buckle and 13-a first connecting shaft;
2-inner shield module, 21-inner shield body, 211-annular groove, 212-through hole, 213-outer step; 22-inner shield upper cover, 221-annular projection, 222-upper conical hole, 223-round ear; 23-a second connecting shaft;
3-a detection cell module, 31-a detection cell main body, 311-an accommodating cavity, 312-a lower tapered hole, 313-a first clamping ring and 32-a first magnetic ring;
4-detector module, 41-detector body, 411-taper, 412-window, 413-light source channel, 414-second snap ring; 42-an induction device, 43-an optical filter, 44-a second magnetic ring, 45-a light source;
5-a microprocessor system;
6-a display screen; 7-sample container.
Detailed Description
In the existing fluorescence detector, the detection cell is usually in a relatively open state, and a light path has larger background noise due to the refraction and reflection phenomena of light; or the light path needs to be recalibrated every time the detector in the fluorescence detector is disassembled and replaced, and the recalibration is usually carried out by a professional, so that the recalibration is complex and tedious. In order to solve the problems, the invention provides an integrated liquid and solid detection pool device and a fluorescence detector, wherein the detection pool device is an integrated liquid and solid detection pool device and is provided with a shielding module, a detection pool module and a detector module, the shielding module comprises a shielding body, the shielding body is provided with a cavity, the bottom of the shielding body is provided with a through hole, and the top of the shielding body is provided with an upper conical hole communicated with the cavity; the detection tank module comprises a detection tank main body and a first magnetic ring arranged on the periphery of the detection tank main body, wherein the upper part of the detection tank main body is provided with a containing cavity for placing a sample container, the lower part of the detection tank main body is provided with a lower conical hole communicated with the containing cavity, the small end of the lower conical hole is positioned above the lower conical hole, the detection tank module is clamped at the bottom through hole in the shielding body, and the lower conical hole of the lower part of the detection tank module extends out of the through hole; the upper end of the detector main body of the detector module is a conical part which is matched with a lower conical hole at the lower part of the detection pool main body and an upper conical hole of the shielding body, and the periphery of the detector main body is provided with a second magnetic ring which is attracted with the first magnetic ring. Preferably, one of the first magnetic ring and the second magnetic ring is a strong magnetic ring (strong magnet), and the other is a soft magnetic ring (i.e. a magnetic ring made of soft magnetic material).
When liquid sample detection is carried out, the detector module is installed at the lower conical hole of the lower portion of the detection pool module, the shielding module is provided with a shielding upper cover and used for covering the upper conical hole of the shielding body to prevent light from entering the shielding body, at the moment, the upper conical hole of the shielding body is coaxial with the lower conical hole of the detection pool main body, the conical small end of the shielding body is arranged oppositely, and the first magnetic ring positioned on the periphery of the detection pool main body and the second magnetic ring positioned on the periphery of the detector main body are attracted to lock the detector module. When solid or powder samples are detected, the shielding upper cover is opened, and the detector module is installed at the upper conical hole of the shielding body, namely the conical part of the detector module is inserted into the upper conical hole.
Specifically, the shielding module comprises an outer shielding module and an inner shielding module, the outer shielding module comprises an outer shielding main body and an outer shielding upper cover (namely a shielding upper cover) hinged with the outer shielding main body, and the outer shielding main body is of a hollow structure; the inner shielding module comprises an inner shielding main body and an inner shielding upper cover hinged with the inner shielding main body, the inner shielding main body is nested in the outer shielding main body to form an integrated structure, the through hole is formed in the bottom of the inner shielding main body, and the upper conical hole is formed in the inner shielding upper cover.
The integrated liquid and solid detection cell device and the fluorescence detector of the present invention will be described in detail with reference to the accompanying drawings and examples.
In the first embodiment shown in fig. 1, the detection cell device 01 of the present invention includes an outer shielding module 1, an inner shielding module 2, a detection cell module 3 and a detector module 4, wherein an inner shielding main body 21 of the inner shielding module 2 is embedded in an outer shielding main body 11 of the outer shielding module 1 to form an integrated structure, the detection cell module 3 is assembled in the inner shielding main body 21, and the detector module 4 is installed at the bottom of the detection cell module 3, wherein:
referring to fig. 1 and 3, the outer shielding body 11 of the outer shielding module 1 is a hollow annular body, the bottom of the inner wall is provided with an annular protrusion 112 for supporting the inner shielding body 21, and the upper end of the outer wall is provided with a snap spring structure 111; the outer shielding module 1 is further provided with an outer shielding upper cover 12, the outer shielding upper cover 12 is hinged to the outer shielding main body 11 through a first connecting shaft 13 (the outer shielding upper cover 12 can rotate around the first connecting shaft 13), the edge of the outer shielding upper cover 12 is provided with a buckle 121, the buckle 121 is matched with a clamp spring structure 111 arranged on the outer shielding main body 11 to be used for buckling the outer shielding upper cover 12 at an upper opening of the outer shielding main body 11, and the clamp spring structure 111 can adopt a commonly-used spring clamp spring structure. In one embodiment, the clamp spring structure 111 includes a clamp spring with a hook on the upper portion and a button connected to the clamp spring, a spring is disposed between the inner side of the clamp spring and the outer shield body 11, when the clamp spring structure is used, the outer shield upper cover 12 is lightly pressed to enable the buckle 121 to be matched and buckled with the clamp spring of the clamp spring structure 111, the button of the clamp spring structure 111 is lightly pressed to enable the clamp spring to compress the spring, the buckle 121 of the outer shield upper cover 12 can be separated from the clamp spring structure 111, and the outer shield upper cover 12 is opened. The outer shield main body 21 is fastened with the outer shield upper cover 12 to realize the light-shielding effect during sample detection. Preferably, the outer shield body 11 is made of a black plastic material or a blackened aluminum alloy material to remove stray light.
The structure of the inner shielding module 2 is shown in fig. 1 and fig. 4, and includes an inner shielding main body 21 and an inner shielding upper cover 22, the inner shielding main body 21 is a cylinder with an upper opening, a through hole 212 is arranged at the bottom, an outer step 213 is arranged at the lower end of the outer wall of the cylinder, and is matched with the inner step 112 of the outer shielding main body 11; the inner shield upper cover 22 is hinged to the inner shield main body through the second connecting shaft 23 (the inner shield upper cover 22 can rotate around the second connecting shaft 23), an annular protrusion 221 is arranged on the inner side of the inner shield upper cover 22 and is matched with an annular groove 211 arranged on the inner shield main body 21, and an upper tapered hole 222 is arranged in the middle of the inner shield upper cover 22 (namely, the large end is located on the outer side of the inner shield upper cover 22, and the small end is located on the inner side of the inner shield upper cover 22) and is used for installing the detector module 4. The inner shield body 21 is nested in the outer shield body 11, and the inner wall and the inner step 112 of the outer shield body 11 are respectively attached to the outer wall and the outer step 213 of the inner shield body 21. When the inner shield upper cover 22 is buckled with the inner shield main body 21, the annular protrusion 221 of the inner shield upper cover 22 is nested with the annular groove of the inner shield main body 21 to achieve the light shielding effect. Preferably, the inner shield body 21 may be made of a black plastic material or a blackened aluminum alloy material to remove stray light; the edge of the inner shield upper cover 22 is provided with a round ear 223 extending outward for opening the inner shield upper cover 22, and the inner wall of the corresponding outer shield main body 11 is provided with a gap matching with the round ear 223.
Referring to fig. 1 and 5, the detection cell module 3 includes a detection cell main body 31 and a first magnetic ring 32 disposed at the periphery of the detection cell main body, the upper portion of the detection cell main body 31 is provided with a receiving cavity 311 for receiving the sample container 7, and the lower portion of the detection cell main body 31 is provided with a lower tapered hole 312 coaxially communicated with the receiving cavity 311 (i.e., the large end of the tapered hole is located below, and the small end is located above). Specifically, the outer periphery of the detection cell main body 31 is provided with a first snap ring 313 so as to be clamped with the inner shielding main body 21, the detection cell main body 31 is clamped at the bottom through hole 212 in the inner shielding main body 21, the first snap ring 313 is attached to the bottom of the inner shielding main body 21, and the lower conical hole 312 of the first snap ring extends out of the through hole. Preferably, the detection cell main body 31 may be made of a black plastic material or a blackened aluminum alloy material to remove stray light.
The structure of the detector module 4 is shown in fig. 1 and fig. 6, and includes a detector main body 41, a second magnetic ring 44 disposed on the periphery of the detector main body, and an induction device 42, a filter 43 and a light source 45 disposed on the detector main body, wherein the upper end of the detector main body 41 is provided with a conical portion 411, the top of the conical portion 411 is provided with a window 412, and the conical portion 411 is matched with the upper conical hole 222 of the inner shielding upper cover 22 and the lower conical hole of the detection cell main body 31; a second snap ring 414 extends outwards from the outer periphery of the detector main body 41, the second magnetic ring 44 is sleeved on the detector main body 41 and limited at the second snap ring 414, and the second magnetic ring 44 and the first magnetic ring 32 can attract each other; the detector body 41 is provided with a cavity with a lower opening, the detector body 41 is provided with a light source channel 413 communicated with the cavity, the light source 45 is arranged in the light source channel 413, light emitted by the light source 45 can penetrate through the window 412, and preferably, the light source 45 is an LED light source and is symmetrically arranged; the cavity opening of the detector body 41 is provided with the sensing device 42 and the optical filter 43 in sequence from outside to inside, the optical filter 43 only allows light with relevant wavelengths to pass through, and the light wave is determined by a sample to be detected. Preferably, the detector body 41 is made of black plastic or blackened aluminum alloy to remove stray light.
In this embodiment, the detector module 4 is installed at the lower portion of the detection cell module 3, that is, the tapered portion 411 of the detector module 4 is in clearance fit with the lower tapered hole 312 at the lower portion of the detection cell module 3, and meanwhile, the second magnetic ring 44 of the detector module 4 and the first magnetic ring 32 of the detection cell module 3 are fixed in an attracting manner. This embodiment is suitable for the detection of liquid sample, and outer shielding main part 11 and outer shielding upper cover 12 lock are locked, prevent the interference of external light.
Preferably, one of the first magnetic ring 32 and the second magnetic ring 44 is a ferromagnetic ring (e.g., a magnetic ring made of ferrite permanent magnetic material, neodymium iron boron permanent magnetic material, alnico, etc.), and the other is a soft magnetic ring (e.g., a magnetic ring made of soft magnetic material such as iron silicon alloy, soft magnetic ferrite, etc.).
Obviously, the second magnetic ring 44 and the first magnetic ring 32 can be attracted to each other, as long as the detector module 4 and the detection cell module 3 are attracted by a magnetic attraction manner under the condition of matching shapes, which both belong to the concept of the present invention, and the installation positions and installation manners of the used adsorbing materials and adsorbing materials are not limited herein.
Referring to fig. 7, the fluorescence detector comprises a detection cell device 01, a microprocessor system 5 and a display screen 6, wherein the display screen 6 and a light source 45 and a sensing device 42 in the detection cell device 01 are electrically connected to the microprocessor system 5. The sample container 7 is placed in the detection cell main body 31 of the detection cell device 01, preferably, the sample container 7 is a disposable micro light-transmitting plastic cup, so that the use is convenient, and the secondary pollution can be avoided. The light source 45 is connected with the sample container 7 and the sensing device 42 in turn through optical paths.
When a sample is detected, light emitted from the light source 45 is irradiated onto a sample in a sample container disposed in the detection cell main body 31 through the window 412, and light generated by excitation of the sample is irradiated onto the sensing device 42 through the optical filter 43. The sensing device 42 converts the optical signal into an electrical signal and transmits the electrical signal to the microprocessor system 5 for processing, so as to obtain a detection result of the sample to be detected, and transmit the detection data and the detection result to the display screen 6 for displaying.
In this embodiment, the light source 45 may be an ultra-high brightness light emitting diode produced by the domestic pioneer photoelectric company, the brightness parameter is above 1000mcd, the power supply mode may be continuous or pulse, and the display screen 6 may be a liquid crystal display screen or a touch screen; the sensing device 42 can be an integrated photoelectric sensor with the model number of S1133 manufactured by the Nippon Korea company, and can also be other integrated photoelectric sensors with the same effect; the microprocessor system 5 is an STC series produced by domestic macrocrystal company, and the main parameters of the microprocessor system are an 8-bit singlechip and an internal memory 64K; the circuits involved in this embodiment are conventional circuits.
In the second embodiment shown in fig. 2, the detection cell device 01 is substantially the same as the first embodiment except that the detector module 4 is mounted on the inner shield upper cover 22, i.e., the tapered portion 411 of the detector module 4 is in clearance fit with the upper tapered hole 222 of the inner shield upper cover 22, and this embodiment is suitable for detection of solid or powder samples, when the outer shield upper cover 12 is opened (the outer shield module 1 in fig. 2 is not shown).
When detecting a solid or powder sample, if the detector module 4 is located below the sample container 7, most of the light emitted by the light source cannot penetrate through the sample due to the reflection or scattering effect of the solid or powder sample on the light, and a large part of the light received by the detector module 4 is reflected or scattered light, not excitation light, which affects the detection accuracy; and the detector module 4 is positioned above the sample container 7 and can be opposite to the sample reacted with the reagent above the sample container 7, and the light received by the detector module 4 is basically exciting light.
The integrated liquid and solid detection pool device and the fluorescence detector have the following characteristics:
1) the invention adopts a two-stage shielding structure of the inner shielding module 2 and the outer shielding module 1, thereby reducing the influence of external light on the detection result as much as possible; the detection cell device is flexible and convenient to use, and the detector module 4 can be directly inserted into the upper conical hole 222 of the upper inner shielding upper cover 22 or directly inserted into the lower conical hole 312 of the lower detection cell module 3;
2) the detector module is locked by adopting shape matching guide and magnetic attraction, so that the automatic calibration of the light path is realized, the operation is simple and convenient, and the operation can be flexibly performed by non-professional operators;
3) the detection cell device 01 can be used for detecting liquid, solid or powder samples, the detection effect can be realized by adopting less samples and reagents, the reagent dosage is reduced, and the cost is saved; the sample container 7 adopts a disposable transparent plastic cup, is convenient to use and has no secondary pollution.
The invention uses two test examples to verify the use effect of the fluorescence detector.
The first test example: detection of mercury content in liquid sample
1) Preparing a sample: injecting 0.1mg of the solution into a sample container 7 by using an injector, adding a drop of mercury test agent, and reacting for 3 minutes;
2) sample loading operation: opening the upper outer shielding cover 12 and the upper inner shielding cover 22 of the integrated liquid and solid detection cell device, placing the sample container 7 with the sample into the accommodating cavity 311 of the detection cell main body 31, and then closing the upper inner shielding body cover 22 and the upper outer shielding cover 12 in sequence; then the conical part 411 of the detector module 4 is inserted into the lower conical hole 312 at the lower part of the detection cell main body, the detector module 4 is locked at the position through the attraction action of the second magnetic ring 44 and the first magnetic ring 32 of the detection cell module, and the sensing device 42 is arranged coaxially with the sample container 7. (the configuration of the detection cell device 01: the light source 45 is a 365nm high-brightness LED, the optical filter 43 is a 430nm band-pass optical filter, and only allows 430nm light to pass through, the sensing device 42 is a photoelectric sensor S1133, and the display screen 6 is a liquid crystal display screen).
3) And (3) quantitative detection: starting up detection, wherein light emitted by a light source 45(365nm high-brightness LED) irradiates a sample in the sample container 7 through a window 412 of the detector module 4 to generate light with a wavelength of 430 nm; filtering by a filter 43 with the wavelength of 430nm to reach the sensing device 42 (a photoelectric sensor S1133); the microprocessor system 5 receives the signal output by the photoelectric sensor S1133 for processing, and the data result is sent to the display screen 6 for display. The whole detection process is about 3 minutes.
Solutions with different mercury concentrations are prepared and used as samples to be detected in sequence, and the detection results are shown in table 1. According to comparison of a mercury concentration fluorescence detection standard database, the detection result in the table 1 is basically consistent with the real result, and the result is reliable by adopting the fluorescence detector.
TABLE 1 results of mercury concentration measurement in solution
| 1 | 2 | 3 | 4 | 5 | 6 | 7 | |
| Mercury concentration (mg/kg) | 0 | 50.0 | 100.0 | 500.0 | 1000.0 | 5000.0 | 10000.0 |
| Fluorescence intensity value | 36 | 3860 | 5670 | 7729 | 9765 | 20184 | 25600 |
The solution may be a cosmetic solution or other liquid sample.
Test example two: detection of aluminum content in powder sample
1) Preparing a sample: putting the powder to be detected into a sample container 7, strickling, adding a drop of aluminum detection reagent, and reacting for 5 minutes;
2) sample loading operation: opening the upper cover 12 and the upper cover 22 of the outer shield of the integrated liquid and solid detection cell device, placing the sample container 7 with the sample into the accommodating cavity 311 of the detection cell main body 31, and then closing the upper cover 22 of the inner shield; then the conical part 411 of the detector module 4 is inserted into the upper conical hole 222 on the inner shielding upper cover 12, the detector module 4 is locked at the position by the attraction effect of the second magnetic ring 44 and the first magnetic ring 32 of the detection cell module and automatically aligned with the sample container 7 in the detection cell module 3, and the sensing device 42 is arranged coaxially with the sample container 7. (the configuration of the detection cell device 01: the light source 45 is a 380nm high-brightness LED, the optical filter 43 is a 530nm band-pass optical filter, only 530nm light is allowed to pass through, the sensing device 42 is a photoelectric sensor S1133, and the display screen 6 is a liquid crystal display screen).
3) And (3) quantitative detection: starting up for detection, wherein an excitation light source emitted by a high-brightness LED at 380nm irradiates a sample in the sample container 7 through a window 412 of the detector module 4 to generate an emission light source with a wavelength of 530 nm; filtering with the 530nm filter 43 to reach the photoelectric sensor S1133; the microprocessor system 5 receives the signal output by the photoelectric sensor S1133 for processing, and the data result is sent to the display screen 6 for display. The whole detection process is about 5 minutes.
A plurality of groups of samples made of powders with different aluminum contents were sequentially tested, and the test results are shown in table 2. According to the comparison of the aluminum concentration fluorescence detection standard database, the detection result in the table 2 is basically consistent with the real result, and the result is reliable by adopting the fluorescence detector.
TABLE 2 measurement of the aluminum content in the powder
| 1 | 2 | 3 | 4 | 5 | 6 | 7 | |
| Aluminum concentration (mg/kg) | 0 | 1.0 | 5.0 | 10.0 | 20.0 | 50.0 | 100.0 |
| Fluorescence intensity value | 123 | 1365 | 2012 | 2351 | 3005 | 3614 | 5231 |
In this example, the powder may be flour or other powder, or may be a solid material such as a puff, steamed bread, twisted cruller, or the like.
The fluorescence detector is simple and convenient to operate and rapid in detection, and a preset detection effect can be realized by using a small reagent.
It will be understood by those skilled in the art that these examples are for illustrative purposes only and are not intended to limit the scope of the present invention, and that various equivalent modifications and changes may be made thereto without departing from the scope of the present invention.
Claims (10)
1. An integrated liquid and solid detection cell device, comprising:
the shielding module comprises a shielding body, wherein the shielding body is provided with a cavity, the bottom of the shielding body is provided with a through hole (212), and the top of the shielding body is provided with an upper tapered hole (222) communicated with the cavity;
the detection cell module (3) comprises a detection cell main body (31), wherein the upper part of the detection cell main body is provided with a containing cavity (311) for placing a sample container (7), the lower part of the detection cell main body is provided with a lower tapered hole (312) communicated with the containing cavity, the containing cavity (311) of the detection cell main body (31) is communicated with the upper tapered hole (222) of the shielding body, the detection cell main body (31) is clamped at a through hole at the bottom in the shielding body, and the lower tapered hole (312) of the lower part of the detection cell main body extends out of the through hole (212); and
the detector module (4) is provided with a detector main body (41), the upper end of the detector main body (41) is provided with a conical part (411), and the conical part (411) is matched with a lower conical hole (312) at the lower part of the detection cell main body (31) and an upper conical hole (222) of the shielding body.
2. An integrated liquid and solid detection cell arrangement according to claim 1, characterized in that the cone (411) of the detector module (4) is mounted in a lower cone hole (312) of the detection cell body (31), the shielding module being provided with a shielding upper cover covering the upper cone hole (222) of the shielding; the detection cell module (3) further comprises a first magnetic ring (32) arranged on the periphery of the detection cell main body, a second magnetic ring (44) attracted with the first magnetic ring is arranged on the periphery of the detector main body (41), and the second magnetic ring (44) is attracted with the first magnetic ring (32) to fix the conical part (411) of the detector main body (41) in the lower conical hole (312) of the detection cell main body (31).
3. An integrated liquid and solid detection cell arrangement according to claim 1, characterized in that the tapered portion (411) of the detector module (4) is mounted at the upper tapered hole (222) of the shield.
4. The integrated liquid and solid detection cell device according to any one of claims 1 to 3, wherein the shielding module is provided with an outer shielding module (1) and an inner shielding module (2), the outer shielding module (1) comprises an outer shielding main body (11) and an outer shielding upper cover (12) hinged with the outer shielding main body, and the outer shielding main body (11) is of a hollow structure; interior shielding module (2) include interior shielding main part (21) and with interior shielding main part articulated interior shielding upper cover (22), interior shielding main part (21) nestification forms the integral type structure in outer shielding main part (11), shielding main part's bottom including through-hole (212) set up, shielding upper cover (22) including last toper hole (222) set up.
5. The integrated liquid and solid detection cell device according to claim 4, wherein the external shielding upper cover (12) is hinged to the external shielding main body (11) through a first connecting shaft (13), and a buckle (121) is arranged at the edge of the external shielding upper cover (12) and is used in cooperation with a clamp spring structure (111) arranged on the external shielding main body (121) to buckle the external shielding upper cover (12) at the upper opening of the external shielding main body (11); the clamp spring structure (111) comprises a clamp spring with a hook at the upper part and a key connected with the clamp spring, and a spring is arranged between the inner side of the clamp spring and the outer shielding main body (11).
6. The integrated liquid and solid detection cell device according to claim 4 or 5, wherein the inner shielding main body (21) is a cylinder with an upper opening, and the lower end of the outer wall of the inner shielding main body is provided with an outer step (213) which is matched and attached with an inner step (112) arranged on the inner wall of the outer shielding main body (11); an annular bulge (221) is arranged on the inner side of the inner shielding upper cover (22) and is matched and embedded with an annular groove (211) arranged on the inner shielding main body (21) when the inner shielding upper cover (22) is buckled with the inner shielding main body (21).
7. The integrated liquid and solid detection cell device according to any one of claims 1 to 6, wherein the detector module (4) further comprises a sensing device (42), an optical filter (43) and a light source (45) arranged on the detector main body (41), the detector main body (41) is provided with a cavity with a lower opening and a light source channel (413) communicated with the cavity, the light source (45) is arranged in the light source channel (413), and light emitted by the light source (45) is transmitted out through a window (412) arranged at the top of the conical part (411) of the detector main body; the cavity opening of the detector main body (41) is sequentially provided with a sensing device (42) and an optical filter (43) from outside to inside, and the optical filter (43) only allows light with relevant wavelengths to pass through.
8. The integrated liquid and solid detection cell device according to any one of claims 1 to 7, wherein the outer shield, the detection cell body (31) and the detector body (41) are made of a black plastic material or a blackened aluminum alloy material.
9. A fluorescence detector comprising a microprocessor system (5) and a detection cell arrangement (01) electrically connected to the microprocessor system (5), characterized in that the detection cell arrangement (01) is an integrated liquid and solid detection cell arrangement according to any one of claims 1 to 8.
10. The fluorescence detector according to claim 9, characterized in that it further comprises a display screen (6), the display screen (6) and the light source (45) and the sensing device (42) of the detection cell arrangement (01) being electrically connected to the microprocessor system (5); the sample container (7) placed in the detection cell main body (31) of the detection cell device (01) is a disposable micro light-transmitting plastic cup.
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| CN201911154307.6A CN110806382B (en) | 2019-11-22 | 2019-11-22 | Integrated liquid and solid detection pool device and fluorescence detector |
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| CN111929258A (en) * | 2020-09-01 | 2020-11-13 | 南京锦华机械制造有限公司 | Food chemical pollution detection device |
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