CN110804535A - Device for storing liquid microbial agent - Google Patents

Device for storing liquid microbial agent Download PDF

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Publication number
CN110804535A
CN110804535A CN201911279879.7A CN201911279879A CN110804535A CN 110804535 A CN110804535 A CN 110804535A CN 201911279879 A CN201911279879 A CN 201911279879A CN 110804535 A CN110804535 A CN 110804535A
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China
Prior art keywords
storage
liquid microbial
microbial inoculant
storage shell
isolation layer
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CN201911279879.7A
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Chinese (zh)
Inventor
王强锋
夏中梅
侯勇
欧阳静祺
刘武
王海涛
彭艳
曾显斌
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Sichuan Lanyue Science & Technology Co Ltd
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Sichuan Lanyue Science & Technology Co Ltd
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Priority to CN201911279879.7A priority Critical patent/CN110804535A/en
Publication of CN110804535A publication Critical patent/CN110804535A/en
Pending legal-status Critical Current

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    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
    • C12M29/00—Means for introduction, extraction or recirculation of materials, e.g. pumps
    • C12M29/04—Filters; Permeable or porous membranes or plates, e.g. dialysis
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
    • C12M37/00—Means for sterilizing, maintaining sterile conditions or avoiding chemical or biological contamination
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
    • C12M37/00—Means for sterilizing, maintaining sterile conditions or avoiding chemical or biological contamination
    • C12M37/02—Filters

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  • Life Sciences & Earth Sciences (AREA)
  • Health & Medical Sciences (AREA)
  • Wood Science & Technology (AREA)
  • Engineering & Computer Science (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Organic Chemistry (AREA)
  • Chemical & Material Sciences (AREA)
  • Zoology (AREA)
  • Biomedical Technology (AREA)
  • Sustainable Development (AREA)
  • Microbiology (AREA)
  • Biochemistry (AREA)
  • General Engineering & Computer Science (AREA)
  • General Health & Medical Sciences (AREA)
  • Genetics & Genomics (AREA)
  • Biotechnology (AREA)
  • Molecular Biology (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)

Abstract

The invention relates to the technical field of a microbial liquid microbial agent storage device, in particular to a device for storing a liquid microbial agent, which comprises: an isolation layer is arranged in the storage shell; the top of the storage shell is provided with an opening; prevent stifled ventilative device from last to including ventilated membrane, cushion chamber and reversal funnel down in proper order, the bottom of reversal funnel with the opening of storage shell body is connected, the cushion chamber with reversal funnel passes through the buffering mouth to be connected, the inner wall of buffering mouth is provided with the boss. The invention solves the problems of packing flatulence and liquid microbial inoculum pollution of the liquid microbial inoculum in the prior art, and has the advantages of reasonable and practical structural design, simplicity, high efficiency, low cost, prolonged storage life of the liquid microbial inoculum and convenient popularization and use.

Description

Device for storing liquid microbial agent
Technical Field
The invention relates to the technical field of microbial liquid microbial agent storage devices, in particular to a device for storing a liquid microbial agent.
Background
In the prior art, the storage mode of the liquid microbial agent has the following problems:
1. the packaging barrel of the liquid microbial agent is not generally subjected to sterilization treatment, because the packaging barrel is mostly not resistant to high temperature and cannot be sterilized at high temperature, and the sterilization cost is too high if the packaging barrel is subjected to a chemical mode or an irradiation mode.
2. The general liquid microbial inoculum is held by a wide-mouth container such as a stacking barrel, the thalli are easy to grow on the liquid surface to form a bacterial film to influence the appearance, the surface is also easy to be polluted by mixed bacteria, and particularly the quality is seriously influenced by the fungal pollution.
3. The method has certain limitation on preventing the pollution of the mixed bacteria by adding sodium benzoate, glycine, potassium sorbate and the like, can be only used for the liquid microbial agent of the bacillus, and the proper amount of preservatives such as sodium benzoate, glycine, potassium sorbate and the like can not kill spores. However, the method can not prevent the mixed bacteria pollution of the non-bacillus liquid microbial agent, and the addition of sodium benzoate, glycine, potassium sorbate and the like can inhibit the liquid microbial agent per se, thereby reducing the effective viable count.
4. The method is only suitable for the preservation of strict aerobic liquid microbial inoculum and cannot solve the gas production problem of the facultative anaerobic liquid microbial inoculum.
5. Under the existing packaging condition, a water film or an oil film is formed on the surface of the breathable film by the liquid microbial inoculum or an isolation layer on the surface, and in addition, a bacterial film formed by the pollution of mixed bacteria also covers the breathable film, so that the ventilation is not smooth.
Disclosure of Invention
In view of the above-mentioned drawbacks of the prior art, it is an object of the present invention to provide a device for storage of a liquid microbial inoculum, which solves the problems of the prior art that the liquid microbial inoculum is prone to package flatulence and contamination of the liquid microbial inoculum. The anti-pollution liquid microbial agent has the advantages of reasonable and practical structural design, simplicity, high efficiency and low cost, can not carry out anti-pollution formula treatment on the liquid microbial agent, can prolong the storage life of the liquid microbial agent, and is convenient to popularize and use.
To achieve the above and other related objects, the present invention provides a device for liquid microbial inoculant storage, comprising:
a storage case having an isolation layer disposed therein; the top of the storage shell is provided with an opening;
prevent stifled ventilative device, prevent stifled ventilative device from last to including ventilated membrane, cushion chamber and reverse funnel down in proper order, reverse funnel's bottom with the opening of storage casing is connected, the cushion chamber with reverse funnel passes through the buffering mouth to be connected, the inner wall of buffering mouth is provided with the boss.
By designing the isolating layer to float above the liquid microbial agent, substances in the isolating layer do not inhibit the liquid microbial agent per se, and the pollution of surface infectious microbes, particularly fungi, is avoided. The packaging barrel does not need to be sterilized, the packaging is not needed to be carried out under strict aseptic conditions, and the blending treatment of anti-pollution and activity inhibition is not needed to be carried out on the liquid microbial agent. Thereby prevent stifled ventilative device and effectively avoid the oscillation of transportation to make liquid microbial inoculum or isolation layer cover and block up the emergence that the ventilated membrane leads to the flatulence, can flow back downwards fast after liquid gets into the cushion chamber, reduce liquid microbial inoculum itself and surface isolation layer to the coverage probability of ventilated membrane, do benefit to gaseous quick discharge.
Further: the lower part of the storage shell is cylindrical, and the upper part of the storage shell is conical.
The tapered structure with the small upper part and the large lower part physically reduces the contact area of the liquid microbial inoculum and air, and reduces the dosage and the pollution probability of the surface isolation layer of the liquid microbial inoculum in the container.
Further: the lower part of the storage shell is cylindrical, and the upper part of the storage shell is provided with an inclined side plate. The tapered structure with the small upper part and the large lower part physically reduces the contact area of the liquid microbial inoculum and air, and reduces the dosage and the pollution probability of the surface isolation layer of the liquid microbial inoculum in the container.
Further: the upper part of the storage shell is provided with a handle. The handle is arranged, so that the lifting is convenient
Further: and a liquid microbial agent is arranged in the cavity of the storage shell, and the isolation layer is covered above the liquid microbial agent.
The liquid microbial inoculum is stored in the container body and sealed by the isolation layer.
Further: the isolating layer is a mixture of a water-insoluble preservative and non-volatile oil after homogeneous emulsification; the water-insoluble preservative is one or a combination of sorbic acid, benzoic acid and methyl p-hydroxybenzoate, and the nonvolatile oil is one or a combination of vegetable oil and paraffin oil with density lower than that of water.
The isolation layer is a homogeneous emulsion formed by one or a combination of water-insoluble preservative, vegetable oil and paraffin oil, can isolate the liquid microbial agent from air, can prevent the microbial agent from being killed by the preservative and maintain a lower metabolic rate, is beneficial to prolonging the retention period of the microbial agent, and can greatly reduce the using amount of the preservative.
Further: the mass ratio of the water-insoluble preservative to the nonvolatile oil in the isolation layer is (0.06-0.5): 100, respectively; the thickness of the isolation layer is less than or equal to 1 mm.
Further: and the boss is provided with a buffer bead.
The inner wall of buffering mouth is provided with the boss, places the buffering pearl that can freely move about on the boss, and liquid flows back downwards fast through the groove channel between two bosss after getting into the cushion chamber, reduces the probability of covering of ventilated membrane with surface isolation layer itself, does benefit to gaseous quick discharge.
Further: the air permeable membrane is a hydrophobic and oleophobic micropore leakage-proof air permeable membrane. The hydrophobic and oleophobic microporous leakproof breathable film can prevent a small amount of liquid and oil entering the buffer cavity under severe shaking conditions from covering the breathable film so as to influence ventilation.
As described above, a device for storage of a liquid microbial inoculant according to the present invention has the following advantageous effects: by designing the isolating layer to float above the liquid microbial agent, substances in the isolating layer do not inhibit the liquid microbial agent per se, and the pollution of surface infectious microbes, particularly fungi, is avoided. The packaging barrel does not need to be sterilized, the packaging is not needed to be carried out under strict aseptic conditions, and the blending treatment of anti-pollution and activity inhibition is not needed to be carried out on the liquid microbial agent. Thereby prevent stifled ventilative device and effectively avoid the oscillation of transportation to make liquid microbial inoculum or isolation layer cover and block up the emergence that the ventilated membrane leads to the flatulence, can flow back downwards fast after liquid gets into the cushion chamber, reduce liquid microbial inoculum itself and surface isolation layer to the coverage probability of ventilated membrane, do benefit to gaseous quick discharge.
Drawings
FIG. 1 is a front view of a device for the storage of a liquid microbial inoculant in example 1 of the present invention.
FIG. 2 is a diagram showing the state of inclination of a device for storage of a liquid microbial inoculum in example 1 of the present invention.
FIG. 3 is a front view of a device for the storage of a liquid microbial inoculant in example 2 of the present invention.
FIG. 4 is a diagram showing the state of inclination of a device for storage of a liquid microbial inoculum in example 2 of the present invention.
FIG. 5 is a side view of a storage housing for the storage of a liquid microbial inoculant in example 2 of the invention.
FIG. 6 is a schematic view of an anti-clogging ventilation device for storing liquid microbial inoculum according to an embodiment of the invention.
FIG. 7 is a schematic view of a buffer port for storing a liquid microbial inoculum in an embodiment of the invention.
Description of the element reference numerals
1-a storage housing; 2-a handle; 3-liquid microbial inoculant; 4-an isolation layer; 5-a gas-permeable membrane; 6-a buffer chamber; 7-a reverse funnel; 8-buffer port; 9-boss; 10-buffer beads.
Detailed Description
The following description of the embodiments of the present invention is provided for illustrative purposes, and other advantages and effects of the present invention will become apparent to those skilled in the art from the present disclosure.
Please refer to fig. 1 to 7. It should be understood that the structures, ratios, sizes, and the like shown in the drawings and described in the specification are only used for matching with the disclosure of the specification, so as to be understood and read by those skilled in the art, and are not used to limit the conditions under which the present invention can be implemented, so that the present invention has no technical significance, and any structural modification, ratio relationship change, or size adjustment should still fall within the scope of the present invention without affecting the efficacy and the achievable purpose of the present invention. In addition, the terms "upper", "lower", "left", "right", "middle" and "one" used in the present specification are for clarity of description, and are not intended to limit the scope of the present invention, and the relative relationship between the terms and the terms is not to be construed as a scope of the present invention.
Example 1
Referring to fig. 1, the present invention provides a device for liquid microbial inoculant storage, comprising:
referring to fig. 1, a storage case 1 is provided, wherein an opening is formed at the top of the storage case 1; the lower part of the storage shell 1 is cylindrical, and the upper part of the storage shell 1 is conical; a handle 2 is arranged at the upper part of the storage shell 1; a liquid microbial agent 3 is arranged in the cavity of the storage shell 1, and the isolation layer 4 covers the liquid microbial agent 3;
the mass ratio of the water-insoluble preservative (sorbic acid) to the nonvolatile oil (vegetable oil) in the isolation layer 4 is 0.5: 100, respectively; the thickness position of the isolation layer is 0.08 mm.
Referring to fig. 6, the anti-blocking and ventilating device comprises a ventilating film 5, a buffer cavity 6 and a reverse funnel 7 in sequence from top to bottom, the bottom of the reverse funnel 7 is connected with the opening of the storage shell 1, the buffer cavity 6 is connected with the reverse funnel 7 through a buffer port 8, referring to fig. 7, a boss 9 is arranged on the inner wall of the buffer port 8, a buffer bead 10 is mounted on the boss 9, and the ventilating film 5 is a hydrophobic and oleophobic microporous leakproof ventilating film.
The working process is as follows: transferring the liquid microbial inoculum 3 into the storage shell 1, adding the isolation layer 4, covering the isolation layer 4 on the surface of the liquid microbial inoculum 3, and connecting the reverse funnel 7 with a buckle.
Example 2
Referring to fig. 3, the present invention provides a device for liquid microbial inoculant storage, comprising:
referring to fig. 3, a storage case 1, an opening is disposed at the top of the storage case 1; referring to fig. 5, the lower portion of the storage casing 1 is cylindrical, and the upper portion of the storage casing 1 is provided with an inclined side plate; a handle 2 is arranged at the upper part of the storage shell 1; a liquid microbial agent 3 is arranged in the cavity of the storage shell 1, and the isolation layer 4 covers the liquid microbial agent 3;
the mass ratio of the water-insoluble preservative (methylparaben) to the nonvolatile oil (paraffin oil) in the isolation layer 4 was 0.06: 100, respectively; the thickness position of isolation layer is 0.05 mm.
Referring to fig. 6, the anti-blocking and ventilating device comprises a ventilating film 5, a buffer cavity 6 and a reverse funnel 7 in sequence from top to bottom, the bottom of the reverse funnel 7 is connected with the opening of the storage shell 1, the buffer cavity 6 is connected with the reverse funnel 7 through a buffer port 8, referring to fig. 7, a boss 9 is arranged on the inner wall of the buffer port 8, a buffer bead 10 is mounted on the boss 9, and the ventilating film 5 is a hydrophobic and oleophobic microporous leakproof ventilating film.
The working procedure was the same as in example 1.
In conclusion, the anti-pollution liquid microbial agent has the advantages of reasonable and practical structural design, simplicity, high efficiency and low cost, can not carry out anti-pollution formula treatment on the liquid microbial agent, can prolong the storage life of the liquid microbial agent, and is convenient to popularize and use. Therefore, the invention effectively overcomes various defects in the prior art and has high industrial utilization value.
The foregoing embodiments are merely illustrative of the principles and utilities of the present invention and are not intended to limit the invention. Any person skilled in the art can modify or change the above-mentioned embodiments without departing from the spirit and scope of the present invention. Accordingly, it is intended that all equivalent modifications or changes which can be made by those skilled in the art without departing from the spirit and technical spirit of the present invention be covered by the claims of the present invention.

Claims (10)

1. A device for liquid microbial inoculant storage, wherein the device for liquid microbial inoculant storage comprises:
a storage case (1), wherein an isolation layer (4) is arranged in the storage case (1); the top of the storage shell (1) is provided with an opening;
prevent stifled ventilative device, prevent stifled ventilative device from last to including ventilated membrane (5), cushion chamber (6) and reverse funnel (7) down in proper order, the bottom of reverse funnel (7) with the opening of storage casing (1) is connected, cushion chamber (6) with reverse funnel (7) are connected through buffering mouth (8), the inner wall of buffering mouth (8) is provided with boss (9).
2. A device for the storage of a liquid microbial inoculant according to claim 1, wherein: the lower part of the storage shell (1) is cylindrical, and the upper part of the storage shell (1) is conical.
3. A device for the storage of a liquid microbial inoculant according to claim 1, wherein: the lower part of the storage shell (1) is cylindrical, and the upper part of the storage shell (1) is provided with an inclined side plate.
4. A device for the storage of a liquid microbial inoculant according to claim 2 or 3, wherein: the upper part of the storage shell (1) is provided with a handle (2).
5. A device for the storage of a liquid microbial inoculant according to claim 1, wherein: the storage shell is characterized in that a liquid microbial agent (3) is installed in a cavity of the storage shell (1), and the isolation layer (4) is covered above the liquid microbial agent (3).
6. A device for the storage of a liquid microbial inoculant according to claim 1 or 5, wherein: the isolating layer (4) is a mixture of a water-insoluble preservative and non-volatile oil after homogeneous emulsification.
7. The homogenized, emulsified mixture of water-insoluble preservative and non-volatile oil of claim 6, wherein: the water-insoluble preservative is at least one of sorbic acid, benzoic acid and methyl p-hydroxybenzoate, and the nonvolatile oil is at least one of vegetable oil and paraffin oil with density less than that of water.
8. A device for the storage of a liquid microbial inoculant according to claim 6, wherein: the mass ratio of the water-insoluble preservative to the nonvolatile oil in the isolation layer (4) is (0.06-0.5): 100, respectively; the thickness of the isolation layer (4) is less than or equal to 1 mm.
9. A device for the storage of a liquid microbial inoculant according to claim 7, wherein: and a buffering bead (10) is arranged on the boss (9).
10. A device for the storage of a liquid microbial inoculant according to claim 1, wherein: the breathable film (5) is a hydrophobic and oleophobic microporous leakage-proof breathable film.
CN201911279879.7A 2019-12-13 2019-12-13 Device for storing liquid microbial agent Pending CN110804535A (en)

Priority Applications (1)

Application Number Priority Date Filing Date Title
CN201911279879.7A CN110804535A (en) 2019-12-13 2019-12-13 Device for storing liquid microbial agent

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Application Number Priority Date Filing Date Title
CN201911279879.7A CN110804535A (en) 2019-12-13 2019-12-13 Device for storing liquid microbial agent

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CN110804535A true CN110804535A (en) 2020-02-18

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Cited By (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN112385657A (en) * 2020-11-24 2021-02-23 郑州新泽生物科技有限公司 Liquid preparation and preparation method and application thereof

Cited By (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN112385657A (en) * 2020-11-24 2021-02-23 郑州新泽生物科技有限公司 Liquid preparation and preparation method and application thereof

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