CN110800848A - Method for preparing shinyleaf yellowhorn leaf tea by enzymolysis method - Google Patents
Method for preparing shinyleaf yellowhorn leaf tea by enzymolysis method Download PDFInfo
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- CN110800848A CN110800848A CN201911135178.6A CN201911135178A CN110800848A CN 110800848 A CN110800848 A CN 110800848A CN 201911135178 A CN201911135178 A CN 201911135178A CN 110800848 A CN110800848 A CN 110800848A
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- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
- A23F—COFFEE; TEA; THEIR SUBSTITUTES; MANUFACTURE, PREPARATION, OR INFUSION THEREOF
- A23F3/00—Tea; Tea substitutes; Preparations thereof
- A23F3/34—Tea substitutes, e.g. matè; Extracts or infusions thereof
Abstract
The invention discloses a method for preparing shinyleaf yellowhorn leaf tea by an enzymatic hydrolysis method, and belongs to the technical field of extraction and preparation of active ingredients of natural plant leaves. The xanthoceras sorbifolia leaf tea is prepared by taking xanthoceras sorbifolia leaves as raw materials and adopting an enzymolysis method, and the xanthoceras sorbifolia leaf tea is prepared by utilizing cellulase, pectinase and compound protease through low-temperature water bath enzymolysis and drying. The method can fully carry out enzymolysis on the xanthoceras sorbifolia leaves, so that effective components such as amino acids, tea polyphenol, caffeine and carbohydrates in the tea leaves are fully leached. The method has the advantages of simple process, convenient operation, good production conditions and low processing cost, and can effectively improve the quality of the xanthoceras sorbifolia leaf tea; opens up a new way for preparing the xanthoceras sorbifolia leaf tea, improves the utilization value of the xanthoceras sorbifolia leaves and realizes the comprehensive utilization of the xanthoceras sorbifolia leaves.
Description
Technical Field
The invention relates to the technical field of extraction and preparation of active ingredients of natural plant leaves, in particular to a method for preparing shinyleaf yellowhorn leaf tea by an enzymatic hydrolysis method.
Background
Chinese tea culture is profound, effective components such as amino acid, tea polyphenol, caffeine and carbohydrate in tea leaves are generally surrounded by cell walls which mainly contain cellulose and pectin substances in dry tea, and under extraction conditions such as hot water soaking, diffusion of the effective components in the tea leaves is hindered by the cell walls, so that leaching of the effective components in the tea leaves is influenced to a certain extent.
Xanthoceras sorbifolia Bunge (Xanthoceras sorbifolia Bunge) is a plant of Xanthoceras of Sapindaceae, and is a tree species unique to China. The root, stem, leaf, flower and fruit of the Chinese medicinal composition can be used as medicines, has the effects of dispelling pathogenic wind, relieving swelling and pain, and the like, and is mainly used for treating diseases such as rheumatic arthritis, wind-heat due to rheumatism, skin wind-heat, infantile enuresis, and the like. Research shows that the shinyleaf yellowhorn oil has pharmacological effects of resisting inflammation and cancer, inhibiting HIV protease, improving learning and memory and the like, has the effects of digesting blood lipid substances, softening blood vessels, eliminating thrombus, blocking subcutaneous adipogenesis and reducing blood fat and cholesterol, and has particularly obvious prevention and treatment effects on cardiovascular and cerebrovascular diseases.
Xanthoceras sorbifolia leaf is rich in bioactive substances, 10 chemical components are detected from xanthoceras sorbifolia leaf, wherein the myricitrin content is 5.53%, and 12 trace elements such as strontium, zinc, barium, boron, iron, copper, manganese and the like, wherein the iron content is 255.5 mu g/g. The utilization value of the xanthoceras sorbifolia leaves is considerable from the analysis of nutriology and medicine. At present, the research on the preparation and drinking methods of xanthoceras sorbifolia leaf tea is little. Therefore, it is a problem to be solved by those skilled in the art to provide a method for preparing xanthoceras sorbifolia leaf tea by an enzymatic hydrolysis method.
Disclosure of Invention
In view of the above, the invention provides a method for preparing xanthoceras sorbifolia leaf tea by an enzymatic hydrolysis method, which takes xanthoceras sorbifolia leaves as raw materials, adopts cellulase, pectinase and compound protease to carry out enzymatic hydrolysis on the xanthoceras sorbifolia leaves, prepares the xanthoceras sorbifolia leaf tea which is convenient to drink, pure in taste and rich in nutrition, and improves the quality of the tea.
In order to achieve the purpose, the invention adopts the following technical scheme:
a method for preparing xanthoceras sorbifolia leaf tea by an enzymatic hydrolysis method comprises the following specific steps:
(1) taking xanthoceras sorbifolia leaves as a raw material, and drying in the shade to obtain dry xanthoceras sorbifolia leaves;
(2) crushing the dry xanthoceras sorbifolia leaves, and sieving the crushed dry xanthoceras sorbifolia leaves with a sieve of 20-200 meshes to obtain xanthoceras sorbifolia leaf raw powder;
(3) weighing cellulase, pectinase and compound protease, and uniformly mixing to obtain mixed enzyme;
(4) balanceTaking the xanthoceras sorbifolia leaf raw powder obtained in the step (2), and mixing the raw powder and the liquid according to a mass-to-volume ratio (kg. L) of 1: 2-1: 40-1) Adding distilled water, uniformly mixing, and adding the mixed enzyme obtained in the step (3); carrying out water bath at 37 ℃ for 1-6 h, and inactivating enzyme at 80-100 ℃ for 5-15 min to obtain the xanthoceras sorbifolia leaf tea soup after enzymolysis; the mass of the mixed enzyme accounts for 0.1-2% of the xanthoceras sorbifolia leaf raw powder;
(5) and drying the xanthoceras sorbifolia leaf tea soup at the temperature of 60-80 ℃ to obtain the xanthoceras sorbifolia leaf tea.
Further, the ratio of the activities of the cellulase, the pectinase and the compound protease in the step (3) is 1:1: 1.
The xanthoceras sorbifolia leaf tea is prepared by taking xanthoceras sorbifolia leaves as raw materials and adopting an enzymolysis method, and the xanthoceras sorbifolia leaf tea is prepared by utilizing cellulase, pectinase and compound protease through low-temperature water bath enzymolysis and drying. The method can fully carry out enzymolysis on the xanthoceras sorbifolia leaves, so that effective components such as amino acids, tea polyphenol, caffeine and carbohydrates in the tea leaves are fully leached. The method has the advantages of simple process, convenient operation, good production conditions and low processing cost, and can effectively improve the quality of the xanthoceras sorbifolia leaf tea; opens up a new way for preparing the xanthoceras sorbifolia leaf tea, improves the utilization value of the xanthoceras sorbifolia leaves and realizes the comprehensive utilization of the xanthoceras sorbifolia leaves.
According to the technical scheme, compared with the prior art, the invention discloses a method for preparing shinyleaf yellowhorn leaf tea by an enzymatic hydrolysis method, which has the following beneficial effects:
(1) the method adopts water as an extraction solvent, carries out water bath enzymolysis on the xanthoceras sorbifolia leaves, and has the advantages of good solvent permeability, high extraction efficiency and simple process flow;
(2) the xanthoceras sorbifolia leaf tea is further prepared from xanthoceras sorbifolia leaves by adopting an enzymolysis method, the process flow is simple, the green and environment-friendly effects are realized, the operation is simple and convenient, the reproducibility is good, and the industrial production can be easily amplified;
(3) the invention provides a new idea for the comprehensive development and utilization of other plant leaf tea.
Drawings
In order to more clearly illustrate the embodiments of the present invention or the technical solutions in the prior art, the drawings used in the description of the embodiments or the prior art will be briefly described below, it is obvious that the drawings in the following description are only embodiments of the present invention, and for those skilled in the art, other drawings can be obtained according to the provided drawings without creative efforts.
FIG. 1 is a graph showing an infrared spectrum of xanthoceras sorbifolia leaf enzymolysis.
Detailed Description
The technical solutions in the embodiments of the present invention will be clearly and completely described below with reference to the drawings in the embodiments of the present invention, and it is obvious that the described embodiments are only a part of the embodiments of the present invention, and not all of the embodiments. All other embodiments, which can be derived by a person skilled in the art from the embodiments given herein without making any creative effort, shall fall within the protection scope of the present invention.
The compound protease is purchased from Shanghai-sourced leaf biotechnology limited, and the enzyme activity of the compound protease is 120 u/mg.
Sensory evaluation criteria
The enzymatic hydrolysis shinyleaf yellowhorn leaf tea prepared in the embodiments 1-3 of the invention is compared with the commercially available shinyleaf yellowhorn leaf tea item by item according to the quality of the corresponding color, aroma, taste and shape of the commercially available shinyleaf yellowhorn leaf tea as a level basis, and the judgment result is graded according to a seven-grade method.
Quality evaluation factor: fragrance (A)0) Color of soup (A)1) And taste (A)3) Leaf bottom (A)4)。
Seven-grade evaluation method
| Seven-gear system | Scoring | Description of the invention |
| Height of | +3 | The difference is large and is obviously better than that of a standard sample |
| Is higher than | +2 | The difference is larger and better than that of a standard sample |
| Is a little higher | +1 | Can be distinguished by careful discrimination and is slightly better than a standard sample |
| Is equivalent to | 0 | Standard sample level |
| Is a little lower | -1 | Can be distinguished by careful discrimination and is slightly worse than a standard sample |
| Is lower than | -2 | The difference is larger and worse than the standard product |
| Is low in | -3 | The difference is large and is obviously different from the marked sample |
The evaluation results were calculated according to the following formula: y is A0+A1+A3+A4
Wherein Y represents the total evaluation score; a. the0，A1，A3，A4Each evaluation factor is scored.
And (4) judging a result: judging that the grade of "-3" obtained from any single evaluation factor is unqualified; the total score is less than or equal to minus 3, and the score is unqualified.
Example 1
A method for preparing xanthoceras sorbifolia leaf tea by an enzymatic hydrolysis method comprises the following specific steps:
(1) taking xanthoceras sorbifolia leaves as a raw material, and drying in the shade to obtain dry xanthoceras sorbifolia leaves;
(2) crushing the dry xanthoceras sorbifolia leaves, and sieving the crushed dry xanthoceras sorbifolia leaves with a 40-mesh sieve to obtain xanthoceras sorbifolia leaf raw powder;
(3) weighing cellulase, pectinase and compound protease, and uniformly mixing to obtain mixed enzyme; the ratio of the activity of the cellulase to the activity of the pectinase to the activity of the compound protease is 1:1: 1;
(4) weighing the xanthoceras sorbifolia leaf raw powder obtained in the step (2) according to the mass-to-volume ratio (kg. L) of 1:10 of the material-to-liquid ratio-1) Adding distilled water, uniformly mixing, and adding the mixed enzyme obtained in the step (3); performing water bath at 37 deg.C for 4h, and inactivating enzyme at 100 deg.C for 10min to obtain enzymolyzed xanthoceras sorbifolia leaf tea soup; the mass of the mixed enzyme accounts for 1% of the raw xanthoceras sorbifolia leaf powder;
(5) and drying the xanthoceras sorbifolia leaf tea soup at 60 ℃ to obtain the xanthoceras sorbifolia leaf tea.
Through detection, the total amino acid content of the obtained enzymatic hydrolysis shinyleaf yellowhorn leaf tea is up to 11.47 percent, the polyphenol content is 2.12 percent, the flavone content is 12.39 percent, and the total evaluation score of the quality of the enzymatic hydrolysis shinyleaf yellowhorn leaf tea is 9 points; the total amino acids of the non-enzymatic hydrolysis shinyleaf yellowhorn leaf tea are only 5.84%, the polyphenol content is 1.92%, the flavone content is 7.13%, and the total quality evaluation score is 4.
Example 2
A method for preparing xanthoceras sorbifolia leaf tea by an enzymatic hydrolysis method comprises the following specific steps:
(1) taking xanthoceras sorbifolia leaves as a raw material, and drying in the shade to obtain dry xanthoceras sorbifolia leaves;
(2) crushing the dry xanthoceras sorbifolia leaves, and sieving the crushed dry xanthoceras sorbifolia leaves with a 200-mesh sieve to obtain xanthoceras sorbifolia leaf raw powder;
(3) weighing cellulase, pectinase and compound protease, and uniformly mixing to obtain mixed enzyme; the ratio of the activity of the cellulase to the activity of the pectinase to the activity of the compound protease is 1:1: 1;
(4) weighingThe raw xanthoceras sorbifolia leaf powder obtained in the step (2) is prepared according to the mass-to-volume ratio (kg. L) of 1:15 of material-to-liquid ratio-1) Adding distilled water, uniformly mixing, and adding the mixed enzyme obtained in the step (3); performing water bath at 37 deg.C for 3h, and inactivating enzyme at 80 deg.C for 15min to obtain enzymolyzed xanthoceras sorbifolia leaf tea soup; the mass of the mixed enzyme accounts for 1.5 percent of the raw xanthoceras sorbifolia leaf powder;
(5) and drying the xanthoceras sorbifolia leaf tea soup at 70 ℃ to obtain the xanthoceras sorbifolia leaf tea.
Through detection, the total amino acid content of the obtained enzymatic hydrolysis shinyleaf yellowhorn leaf tea is up to 15.04 percent, the polyphenol content is 2.75 percent, the flavone content is 14.95 percent, and the total evaluation score of the quality of the enzymatic hydrolysis shinyleaf yellowhorn leaf tea is 10 minutes; the total amino acids of the non-enzymatic hydrolysis shinyleaf yellowhorn leaf tea are only 5.84%, the polyphenol content is 1.92%, the flavone content is 7.13%, and the total quality evaluation score is 4.
Example 3
A method for preparing xanthoceras sorbifolia leaf tea by an enzymatic hydrolysis method comprises the following specific steps:
(1) taking xanthoceras sorbifolia leaves as a raw material, and drying in the shade to obtain dry xanthoceras sorbifolia leaves;
(2) crushing the dry xanthoceras sorbifolia leaves, and sieving the crushed dry xanthoceras sorbifolia leaves with a 100-mesh sieve to obtain xanthoceras sorbifolia leaf raw powder;
(3) weighing cellulase, pectinase and compound protease, and uniformly mixing to obtain mixed enzyme; the ratio of the activity of the cellulase to the activity of the pectinase to the activity of the compound protease is 1:1: 1;
(4) weighing the xanthoceras sorbifolia leaf raw powder obtained in the step (2) according to the mass-to-volume ratio (kg.L) of 1:20 of the material-to-liquid ratio-1) Adding distilled water, uniformly mixing, and adding the mixed enzyme obtained in the step (3); performing water bath at 37 deg.C for 5h, and inactivating enzyme at 90 deg.C for 8min to obtain enzymolyzed xanthoceras sorbifolia leaf tea soup; the mass of the mixed enzyme accounts for 0.5 percent of the xanthoceras sorbifolia leaf raw powder;
(5) and drying the xanthoceras sorbifolia leaf tea soup at 80 ℃ to obtain the xanthoceras sorbifolia leaf tea.
Through detection, the total amino acid content of the obtained enzymatic hydrolysis shinyleaf yellowhorn leaf tea is up to 7.09 percent, the polyphenol content is 2.06 percent, the flavone content is 9.78 percent, and the total evaluation score of the quality of the enzymatic hydrolysis shinyleaf yellowhorn leaf tea is 5 points; the total amino acids of the non-enzymatic hydrolysis shinyleaf yellowhorn leaf tea are only 5.84%, the polyphenol content is 1.92%, the flavone content is 7.13%, and the total quality evaluation score is 4.
The infrared spectrogram of the enzymolyzed and soaked shinyleaf yellowhorn (figure 1) is further compared, and the infrared absorption peak intensity of the enzymolyzed shinyleaf yellowhorn leaf is obviously higher than that of the soaked shinyleaf yellowhorn leaf, particularly in the range of 3430cm-1And 1247cm-1The method shows that the enzymolytic xanthoceras sorbifolia leaf sample contains more hydroxyl active functional groups and more amino functional groups, and shows that the active ingredients in the xanthoceras sorbifolia leaf can be more fully extracted by the enzymolysis method.
The previous description of the disclosed embodiments is provided to enable any person skilled in the art to make or use the present invention. Various modifications to these embodiments will be readily apparent to those skilled in the art, and the generic principles defined herein may be applied to other embodiments without departing from the spirit or scope of the invention. Thus, the present invention is not intended to be limited to the embodiments shown herein but is to be accorded the widest scope consistent with the principles and novel features disclosed herein.
Claims (2)
1. A method for preparing shinyleaf yellowhorn leaf tea by an enzymatic hydrolysis method is characterized by comprising the following specific steps:
(1) taking xanthoceras sorbifolia leaves as a raw material, and drying in the shade to obtain dry xanthoceras sorbifolia leaves;
(2) crushing the dry xanthoceras sorbifolia leaves, and sieving the crushed dry xanthoceras sorbifolia leaves with a sieve of 20-200 meshes to obtain xanthoceras sorbifolia leaf raw powder;
(3) weighing cellulase, pectinase and compound protease, and uniformly mixing to obtain mixed enzyme;
(4) weighing the xanthoceras sorbifolia leaf raw powder obtained in the step (2), adding distilled water according to the mass-volume ratio of 1: 2-1: 40, uniformly mixing, and adding the mixed enzyme obtained in the step (3); carrying out water bath at 37 ℃ for 1-6 h, and inactivating enzyme at 80-100 ℃ for 5-15 min to obtain the xanthoceras sorbifolia leaf tea soup after enzymolysis; the mass of the mixed enzyme accounts for 0.1-2% of the xanthoceras sorbifolia leaf raw powder;
(5) and drying the xanthoceras sorbifolia leaf tea soup at the temperature of 60-80 ℃ to obtain the xanthoceras sorbifolia leaf tea.
2. The method for preparing xanthoceras sorbifolia leaf tea by an enzymatic hydrolysis method according to claim 1, wherein the ratio of the activities of the cellulase, the pectinase and the compound protease in the step (3) is 1:1: 1.
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Citations (4)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| US20100272857A1 (en) * | 2007-09-26 | 2010-10-28 | Suntory Holdings Limited | Tea extract, tea beverage, and method of making the same |
| CN103918814A (en) * | 2013-06-04 | 2014-07-16 | 北京农学院 | Xanthoceras sorbifolia leaf tea and application thereof |
| CN106260248A (en) * | 2016-08-10 | 2017-01-04 | 仲恺农业工程学院 | A kind of tea extract and preparation method thereof |
| CN106665966A (en) * | 2016-07-08 | 2017-05-17 | 清华大学 | Herbal tea and preparation method thereof |
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Patent Citations (4)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| US20100272857A1 (en) * | 2007-09-26 | 2010-10-28 | Suntory Holdings Limited | Tea extract, tea beverage, and method of making the same |
| CN103918814A (en) * | 2013-06-04 | 2014-07-16 | 北京农学院 | Xanthoceras sorbifolia leaf tea and application thereof |
| CN106665966A (en) * | 2016-07-08 | 2017-05-17 | 清华大学 | Herbal tea and preparation method thereof |
| CN106260248A (en) * | 2016-08-10 | 2017-01-04 | 仲恺农业工程学院 | A kind of tea extract and preparation method thereof |
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