CN110800610B - Water chestnut strong seedling culture medium and water chestnut seedling field transplanting method thereof - Google Patents
Water chestnut strong seedling culture medium and water chestnut seedling field transplanting method thereof Download PDFInfo
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- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01H—NEW PLANTS OR NON-TRANSGENIC PROCESSES FOR OBTAINING THEM; PLANT REPRODUCTION BY TISSUE CULTURE TECHNIQUES
- A01H4/00—Plant reproduction by tissue culture techniques ; Tissue culture techniques therefor
- A01H4/001—Culture apparatus for tissue culture
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- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01G—HORTICULTURE; CULTIVATION OF VEGETABLES, FLOWERS, RICE, FRUIT, VINES, HOPS OR SEAWEED; FORESTRY; WATERING
- A01G22/00—Cultivation of specific crops or plants not otherwise provided for
- A01G22/25—Root crops, e.g. potatoes, yams, beet or wasabi
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- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01G—HORTICULTURE; CULTIVATION OF VEGETABLES, FLOWERS, RICE, FRUIT, VINES, HOPS OR SEAWEED; FORESTRY; WATERING
- A01G24/00—Growth substrates; Culture media; Apparatus or methods therefor
- A01G24/10—Growth substrates; Culture media; Apparatus or methods therefor based on or containing inorganic material
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- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01H—NEW PLANTS OR NON-TRANSGENIC PROCESSES FOR OBTAINING THEM; PLANT REPRODUCTION BY TISSUE CULTURE TECHNIQUES
- A01H4/00—Plant reproduction by tissue culture techniques ; Tissue culture techniques therefor
- A01H4/008—Methods for regeneration to complete plants
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Abstract
The invention discloses a water chestnut strong seedling culture medium and a water chestnut seedling field transplanting method thereof, wherein the water chestnut strong seedling culture medium comprises the following components in parts by weight: 4 to 5 parts of MS culture medium dry powder, 30 to 40 parts of cane sugar and KNO 3 0.4-0.6 parts of (NH 4) 2 SO 4 2 to 4 portions of deionized water and 800 to 1000 portions of deionized water. The method for transplanting the water chestnut seedlings in the field comprises the following steps: loosening a tissue culture bottle cap of a strong water chestnut seedling cultured by using a water chestnut strong seedling culture medium, carrying out environmental adaptability exercise in a culture room for 7 days, removing the bottle cap, moving the water chestnut strong seedling culture bottle to the room, placing the water chestnut strong seedling culture bottle for 30 days, taking the tissue culture seedling out of the culture bottle, changing water every 5 days for 30 days, washing the culture medium clean by using clear water, and transplanting the culture medium into a culture medium; transplanting the water chestnut tissue culture seedlings tentatively planted to survive in the step (1) into a field. The water chestnut tissue culture seedlings with the culture medium can generate root systems in the strong seedling process, a rooting culture step is not needed independently, and the field transplanting method can improve the field transplanting survival rate.
Description
Technical Field
The invention relates to a water chestnut strong seedling culture medium, in particular to a water chestnut strong seedling culture medium and a water chestnut seedling field transplanting method thereof.
Background
Water chestnut [ eelocharas turberosa (Roxb.) et Schult ] is a perennial shallow water plant of Cyperaceae (Cyperaceae) water chestnut (Eleocharis R Br.), also called water chestnut, chinese chestnut, ting Zhu, black-skinned yam and the like, is originally produced in south China and India, has more than 2000 years of cultivation history in China, has fresh and sweet taste, crisp meat quality and refreshing taste, eliminates dregs, is one of important aquatic vegetables which can be used as both medicine and food and vegetables, has rich nutrition and health care values, also has the effects of clearing heat, reducing phlegm and eliminating food stagnation, can be processed into products such as starch, can, maltose and the like, can also be used as a wine-making raw material, can be used as a feed, and is also used in China as a large country for producing water chestnuts and an export large country. At present, the research of water chestnut at home and abroad mainly comprises sequencing analysis of a water chestnut bulb development process transcriptome, identification and characteristic research of novel viruses, high-quality and high-efficiency cultivation, new variety breeding, storage physiology, processing, flower organ observation, pollen activity detection, tissue culture, in-vitro rapid propagation and the like. The tissue culture and in vitro rapid propagation mainly focus on the aspects of callus induction, stem tip tissue culture, efficient propagation of tissue culture seedlings in TIBs, obtaining of test-tube tissue culture seedlings, corm induction of water chestnuts in test tubes, efficient propagation of the tissue culture seedlings, field transplanting effect research of the water chestnut tissue culture seedlings and the like.
The water chestnuts are also one of the main aquatic vegetables in Anhui province, the cultivation area is about 10 ten thousand mu, the local germplasm resources are rich, and part of the water chestnuts are local famous products, such as the poplar and willow water chestnuts, none of the water chestnuts, shucheng water chestnuts and the like, wherein the poplar and willow water chestnuts belong to national geographical sign protection products. In the main production area of water chestnut in Anhui province, the phenomena of petiole fineness, small nodulite and even no nodulite occur due to long-term adoption of water chestnut corms for breeding seedlings and virus infection, so that some water chestnut germplasm resources with excellent properties have the problems of sexual degeneration, reduced disease resistance and stress resistance, reduced quality and yield, plant growth dwarfing and no nodulization caused by the appearance of male water chestnut in production and the like, and the stem tip culture provides an effective solution for the problem of sexual degeneration of asexual propagation crops. The problems of low multiplication coefficient, low multiplication speed, serious browning of cut materials, low field transplanting survival rate of tissue culture seedlings, high production cost and the like exist in the conventional rapid propagation process of the water chestnuts, and the large-scale production of the water chestnut tissue culture seedlings is restricted. In the tissue culture process, strong seedling culture is an indispensable step for the production of high-quality seedlings, and has certain influence on the later-stage transplanting survival rate. Therefore, through researching the water chestnut strong seedling culture medium, the technical support can be provided for the production of high-quality seedlings, the reduction of the production cost and the industrial production of the water chestnut tissue culture seedlings, and meanwhile, the temporary planting of the tissue culture seedlings is carried out before the transplanting, so that the field transplanting survival rate is favorably improved.
Disclosure of Invention
The invention aims to provide a water chestnut strong seedling culture medium and a water chestnut seedling field transplanting method thereof.
In order to achieve the purpose, the invention provides the following technical scheme:
the water chestnut strong seedling culture medium comprises the following components in parts by weight: 4-5 parts of MS culture medium dry powder, 30-40 parts of cane sugar and KNO 3 0.4 to 0.6 portion of (NH) 4 ) 2 SO 4 2 to 4 portions of deionized water and 800 to 1000 portions of deionized water.
Preferably, the preparation method of the water chestnut strong seedling culture medium comprises the following steps: accurately weighing corresponding parts by weight of MS culture medium dry powder and sucrose respectively, heating the accurately weighed deionized water to boil, sequentially adding the accurately weighed MS culture medium dry powder and sucrose into the deionized water, and respectively adding KNO (potassium permanganate) into the two after the two are completely dissolved 3 And (NH) 4 ) 2 SO 4 Adjusting pH to 5.8, and sterilizing with high pressure steam at 116 deg.C for 30min to obtain corm Eleocharitis strong seedling culture medium.
Preferably, the chufa strong seedling culture medium is used for cultureThe method for strengthening the seedlings of the water chestnuts comprises the following steps: cutting the proliferated cluster buds into 2.5-3.0 cm and 3-4 leaf-shaped stems as a cluster, inoculating the cluster buds into a water chestnut strong seedling culture medium for strong seedling culture, and setting the culture temperature at 23-27 ℃ and the illumination intensity at 30 mu mol/m -2 ·s -1 The illumination time is 14 h.d -1 The relative humidity is 50-70%.
Preferably, the method for transplanting the water chestnut seedlings in the field comprises the following steps:
(1) Temporary planting of the water chestnut seedlings: loosening the tissue culture bottle cap of the strong water chestnut seedling cultured by the strong seedling culture method of claim 3, carrying out environmental adaptability exercise in a culture room for 7 days, removing the bottle cap, moving the tissue culture seedling to the room, placing the tissue culture seedling for 30 days, taking the tissue culture seedling out of the culture bottle, changing water every 5 days for 30 days, washing the culture medium with clear water, and transplanting the culture medium into a culture medium;
(2) Transplanting the water chestnut tissue culture seedlings tentatively planted to survive in the step (1) into a field.
Preferably, the culture medium is selected from two of garden soil, commodity medium and rice husk.
Preferably, the culture medium consists of garden soil and a commodity medium, and the volume ratio of the garden soil to the commodity medium is 1.
Compared with the prior art, the invention has the beneficial effects that:
1) Compared with the existing seedling strengthening method, the water chestnut seedling strengthening method has the following advantages: firstly, the water chestnut strong seedling culture medium can ensure that the water chestnut leaf-shaped stems are thick and strong, the thickness is increased by 27.74 percent compared with a contrast, the uniformity is high, and the growth of the water chestnut strong seedling culture medium can be accelerated; secondly, in the process of strong seedlings, the tissue culture seedlings of the water chestnuts can generate root systems without independently carrying out a rooting culture step, the average number of generated roots is 15.11, which is 23.35 percent more than that of the reference, the culture period is shortened by about 20d, and the time cost is saved; thirdly, the seedling strengthening method does not need to use plant hormone, is safer to produce and apply the tissue culture seedlings, and saves a part of production cost; fourthly, KNO used by the strong seedling method 3 And (NH) 4 ) 2 SO 4 The total cost is lower than the price of the conventionally used paclobutrazol (PP 333), the operation is relatively simple, and the large-scale application in production is easyUse, and improper use of paclobutrazol (PP 333) may cause environmental damage.
2) The cultivation medium can improve the survival rate of the temporary planting of the water chestnut tissue culture seedlings, and after the commodity medium is added, the air permeability and the water retention of the cultivation medium are improved, so that the growth of root systems is facilitated. When the cultivation medium consists of garden soil and commodity medium with the volume ratio of 1.
3) By adopting the field transplanting method, the water chestnut tissue culture seedlings which are temporarily planted to survive are transplanted to the field, the survival rate reaches 100 percent and is 53.07 percent higher than the survival rate of the transplantation which is not temporarily planted.
Detailed Description
The technical solutions in the embodiments of the present invention will be described clearly and completely below, and it is obvious that the described embodiments are only a part of the embodiments of the present invention, and not all embodiments. All other embodiments, which can be obtained by a person skilled in the art without making any creative effort based on the embodiments in the present invention, belong to the protection scope of the present invention.
Example 1
The preparation method of the water chestnut strong seedling culture medium comprises the following steps: accurately weighing 4.74g of MS culture medium dry powder and 30g of cane sugar, heating 1000ml of deionized water to boil, sequentially adding the accurately weighed 4.74g of MS culture medium dry powder and 30g of cane sugar, and respectively adding KNO (potassium permanganate) after the two are completely dissolved 3 And (NH) 4 ) 2 SO 4 Keeping the ratio of the quantity concentration of the substances to be 1 3 And (NH) 4 ) 2 SO 4 The concentration treatments of (2) were 1000ml and repeated 4 times. Sterilizing with high pressure steam at 116 deg.C for 30min.
Cutting the proliferated cluster buds into 2.5-3.0 cm and 3-4 leaf-shaped stems as a cluster, inoculating the cluster buds into a water chestnut strong seedling culture medium for strong seedling culture, and setting the culture temperature at 23-27 ℃ and the illumination intensity at 30 mu mol/m -2 ·s -1 The illumination time is 14 h.d -1 The relative humidity is 50-70%.
Examples 2 to 3
Examples 2-3 differ from example 1 in that: KNO 3 And (NH) 4 ) 2 SO 4 The ratio of the mass concentration of the substances is 1.
Comparative examples 1 to 4
Comparative examples 1 to 4 differ from example 1 in that: KNO 3 And (NH) 4 ) 2 SO 4 The ratio of the mass concentration of the substance (1).
Comparative example
The comparative example differs from example 1 in that: KNO 3 And (NH) 4 ) 2 SO 4 The ratio of the mass concentration of the substances (1) to (2) is 0.
The following table shows the growth conditions of the chufa seedlings cultured in examples 1-3, comparative examples 1-4 and comparative example:
TABLE 1 Effect of different chufa media on chufa strong seedling culture
The KNO was maintained as shown by the analysis of variance and multiple comparisons (Table 1) 3 Constant concentration with (NH) 4 ) 2 SO 4 The stem thickness showed a tendency to increase gradually at higher concentrations, wherein in KNO 3 :(NH 4 ) 2 SO 4 When the mass concentration ratio of substances is 1; maintenance (NH) 4 ) 2 SO 4 Constant concentration with KNO 3 The increase in concentration indicates a thick stemTrend of falling-rising-falling, wherein in (NH) 4 ) 2 SO 4 :KNO 3 When the mass concentration ratio of the substances was 1. Comprehensively comparing to obtain the KNO in the water chestnut strong seedling culture medium 3 :(NH 4 ) 2 SO 4 The mass concentration ratio of substances is 1:2 to 1: and 4, the leafy stems are thick, strong, emerald green and fast in growth. In addition, the water chestnut tissue culture seedlings can generate root systems in the strong seedling process, the rooting culture step is not needed independently, and meanwhile, the plant hormone is not needed, so that part of cost can be saved.
Example 4
A method for transplanting water chestnut seedlings in a field comprises the following steps:
(1) Temporary planting of the chufa seedlings: loosening a tissue culture bottle cap of a strong water chestnut seedling cultured by a strong seedling, carrying out environmental adaptability exercise for 7 days in a culture room, removing the bottle cap, moving the water culture bottle to the room, placing the water culture bottle for 30 days, taking the tissue culture seedling out of the culture bottle, changing water every 5 days in the 30 days, washing a culture medium with clear water, and transplanting the culture medium into a culture medium, wherein the culture medium consists of garden soil and a commodity medium (the commodity medium adopted in the embodiment is a special medium for Xingxing-oriented agricultural brand seedling culture produced by Jiangsu Xingnong matrix science and technology Limited) with the volume ratio of 1;
(2) Transplanting the water chestnut tissue culture seedlings which are temporarily planted to survive in the step (1) into a field.
Transplanting the temporarily planted and alive water chestnut tissue culture seedlings to a field, wherein the survival rate reaches 100%.
Example 5
This embodiment is different from embodiment 4 in that: the culture medium is composed of a commodity medium and rice hulls in a volume ratio of 1.
Example 6
This embodiment is different from embodiment 4 in that: the culture medium consists of garden soil and rice hulls in a volume ratio of 1.
The strong chufa tissue culture seedlings cultured by the strong seedlings are respectively subjected to temporary planting in the culture mediums of the embodiments 4 to 6 to carry out the comparison test of the temporary planting survival rate, and the temporary planting survival rate of the chufa tissue culture seedlings is shown in the table 2:
TABLE 2 survival rate of tissue culture seedlings of water chestnut treated by different culture mediums
| Transplanting substrate proportion | Survival rate/% | Growth after 30d | |
| Example 4 | Garden soil commercial substrate =1 | 100.00％aA | Thick and strong leafy stem and green |
| Example 5 | Commercial substrate rice husk =1 | 90.00％bA | The leaf-like stem is thin and light green |
| Example 6 | Garden soil, rice husk =1 | 73.33％cB | Thin and weak leaf-like stem, yellow-green |
The results of the analysis of variance and multiple comparisons show that (table 2), when the volume ratio of garden soil to the commercial substrate is 1.
TABLE 3 comparison of field transplanting survival rates of water chestnut seedlings with and without temporary planting
| Treatment of | Survival rate of field transplantation/%) |
| Temporary planting | 100.00％aA |
| Not to be temporarily planted | 65.33％bB |
Note: directly transplanting the tissue culture seedling to a field after hardening off the seedling without temporary planting.
Results of variance analysis and multiple comparison show that (Table 3) the highest survival rate of the temporarily planted chufa seedlings after being transplanted to a field reaches 100 percent, and the survival rate is remarkably superior to that of the chufa seedlings which are not temporarily planted.
The foregoing is merely exemplary and illustrative of the present invention and various modifications, additions and substitutions may be made by those skilled in the art to the specific embodiments described without departing from the scope of the present invention as defined in the accompanying claims.
Claims (6)
1. The water chestnut strong seedling culture medium is characterized by comprising the following components in parts by weight: 4-5 parts of MS culture medium dry powder, 30-40 parts of cane sugar and KNO 3 0.4 to 0.6 portion of (NH) 4 ) 2 SO 4 2 to 4 portions of deionized water and 800 to 1000 portions of deionized water.
2. The preparation method of the water chestnut strong seedling culture medium according to claim 1, characterized by comprising the following steps: accurately weighing corresponding parts by weight of MS culture medium dry powder and sucrose respectively, heating the accurately weighed deionized water to boiling, sequentially adding the accurately weighed MS culture medium dry powder and sucrose into the deionized water, and respectively adding KNO (potassium permanganate) into the mixture after the MS culture medium dry powder and sucrose are completely dissolved 3 And (NH) 4 ) 2 SO 4 Adjusting pH to 5.8, and sterilizing with high pressure steam at 116 deg.C for 30min to obtain corm Eleocharitis strong seedling culture medium.
3. The method for culturing the strong chufa seedlings by using the strong chufa seedling culture medium of claim 1, which comprises the following steps of: cutting the proliferated cluster buds into 2.5-3.0 cm and 3-4 leaf-shaped stems as a cluster, inoculating the cluster buds into a water chestnut strong seedling culture medium for strong seedling culture, and setting the culture temperature at 23-27 ℃ and the illumination intensity at 30 mu mol/m -2 ·s -1 The illumination time is 14 h.d -1 The relative humidity is 50-70%.
4. A method for transplanting water chestnut seedlings in a field is characterized by comprising the following steps:
(1) Temporary planting of the chufa seedlings: the method for culturing the strong chufa seedlings by using the strong chufa seedling culture medium of claim 3 comprises the steps of culturing the strong chufa seedlings, putting the cultured strong chufa seedlings into a tissue culture bottle, loosening the bottle cap of the tissue culture bottle, carrying out environmental adaptability exercise in a culture room for 7 days, removing the bottle cap, moving the tissue culture bottle to a room, placing the tissue culture bottle for 30 days, taking the tissue culture seedlings out of the culture bottle, changing water every 5 days in the 30 days, washing the culture medium with clear water, and transplanting the tissue culture bottle into a culture medium;
(2) Transplanting the water chestnut tissue culture seedlings tentatively planted to survive in the step (1) into a field.
5. The method for field transplantation of water chestnut seedlings according to claim 4, wherein the method comprises the following steps: the culture medium is selected from two of garden soil, commodity medium and rice husk.
6. The method for field transplantation of water chestnut seedlings according to claim 5, wherein the method comprises the following steps: the cultivation substrate consists of garden soil and a commodity substrate, and the volume ratio of the garden soil to the commodity substrate is 1.
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Citations (1)
| Publication number | Priority date | Publication date | Assignee | Title |
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| CN103688862A (en) * | 2013-12-16 | 2014-04-02 | 广西壮族自治区农业科学院生物技术研究所 | Method for efficiently propagating water chestnut tissue culture seedlings |
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| Publication number | Priority date | Publication date | Assignee | Title |
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| CN103688862A (en) * | 2013-12-16 | 2014-04-02 | 广西壮族自治区农业科学院生物技术研究所 | Method for efficiently propagating water chestnut tissue culture seedlings |
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