CN110791461A - Acinetobacter calcoaceticus and application thereof - Google Patents
Acinetobacter calcoaceticus and application thereof Download PDFInfo
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- CN110791461A CN110791461A CN201911263232.5A CN201911263232A CN110791461A CN 110791461 A CN110791461 A CN 110791461A CN 201911263232 A CN201911263232 A CN 201911263232A CN 110791461 A CN110791461 A CN 110791461A
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- 241000588624 Acinetobacter calcoaceticus Species 0.000 title claims abstract description 44
- 239000002351 wastewater Substances 0.000 claims abstract description 27
- PAYRUJLWNCNPSJ-UHFFFAOYSA-N Aniline Chemical compound NC1=CC=CC=C1 PAYRUJLWNCNPSJ-UHFFFAOYSA-N 0.000 claims abstract description 22
- 238000004043 dyeing Methods 0.000 claims abstract description 22
- 238000000034 method Methods 0.000 claims abstract description 8
- 238000004321 preservation Methods 0.000 claims abstract description 6
- VYZAMTAEIAYCRO-UHFFFAOYSA-N Chromium Chemical compound [Cr] VYZAMTAEIAYCRO-UHFFFAOYSA-N 0.000 claims abstract description 4
- 150000001555 benzenes Chemical class 0.000 claims abstract description 4
- 239000000843 powder Substances 0.000 claims description 14
- FAPWRFPIFSIZLT-UHFFFAOYSA-M Sodium chloride Chemical compound [Na+].[Cl-] FAPWRFPIFSIZLT-UHFFFAOYSA-M 0.000 claims description 10
- XLYOFNOQVPJJNP-UHFFFAOYSA-N water Substances O XLYOFNOQVPJJNP-UHFFFAOYSA-N 0.000 claims description 10
- UHOVQNZJYSORNB-UHFFFAOYSA-N Benzene Chemical compound C1=CC=CC=C1 UHOVQNZJYSORNB-UHFFFAOYSA-N 0.000 claims description 9
- 239000001963 growth medium Substances 0.000 claims description 9
- 230000001580 bacterial effect Effects 0.000 claims description 8
- 238000000855 fermentation Methods 0.000 claims description 6
- 230000004151 fermentation Effects 0.000 claims description 6
- 238000005273 aeration Methods 0.000 claims description 5
- QVGXLLKOCUKJST-UHFFFAOYSA-N atomic oxygen Chemical compound [O] QVGXLLKOCUKJST-UHFFFAOYSA-N 0.000 claims description 5
- 239000007788 liquid Substances 0.000 claims description 5
- 229910052760 oxygen Inorganic materials 0.000 claims description 5
- 239000001301 oxygen Substances 0.000 claims description 5
- 239000011780 sodium chloride Substances 0.000 claims description 5
- 229910021578 Iron(III) chloride Inorganic materials 0.000 claims description 4
- 229940041514 candida albicans extract Drugs 0.000 claims description 4
- 238000007789 sealing Methods 0.000 claims description 4
- 239000012138 yeast extract Substances 0.000 claims description 4
- 239000003795 chemical substances by application Substances 0.000 claims description 3
- 229910052943 magnesium sulfate Inorganic materials 0.000 claims description 3
- CSNNHWWHGAXBCP-UHFFFAOYSA-L magnesium sulphate Substances [Mg+2].[O-][S+2]([O-])([O-])[O-] CSNNHWWHGAXBCP-UHFFFAOYSA-L 0.000 claims description 3
- 235000015278 beef Nutrition 0.000 claims description 2
- 238000012258 culturing Methods 0.000 claims description 2
- 239000000284 extract Substances 0.000 claims description 2
- 239000002609 medium Substances 0.000 claims description 2
- 238000001694 spray drying Methods 0.000 claims description 2
- UXVMQQNJUSDDNG-UHFFFAOYSA-L Calcium chloride Chemical compound [Cl-].[Cl-].[Ca+2] UXVMQQNJUSDDNG-UHFFFAOYSA-L 0.000 claims 1
- BFNBIHQBYMNNAN-UHFFFAOYSA-N ammonium sulfate Chemical compound N.N.OS(O)(=O)=O BFNBIHQBYMNNAN-UHFFFAOYSA-N 0.000 claims 1
- 229910052921 ammonium sulfate Inorganic materials 0.000 claims 1
- 239000001110 calcium chloride Substances 0.000 claims 1
- 229910001628 calcium chloride Inorganic materials 0.000 claims 1
- ZPWVASYFFYYZEW-UHFFFAOYSA-L dipotassium hydrogen phosphate Chemical compound [K+].[K+].OP([O-])([O-])=O ZPWVASYFFYYZEW-UHFFFAOYSA-L 0.000 claims 1
- 229910000396 dipotassium phosphate Inorganic materials 0.000 claims 1
- 238000001035 drying Methods 0.000 claims 1
- 230000001681 protective effect Effects 0.000 claims 1
- 241000894007 species Species 0.000 claims 1
- 230000000813 microbial effect Effects 0.000 abstract description 3
- 238000012216 screening Methods 0.000 description 7
- XNGIFLGASWRNHJ-UHFFFAOYSA-N phthalic acid Chemical compound OC(=O)C1=CC=CC=C1C(O)=O XNGIFLGASWRNHJ-UHFFFAOYSA-N 0.000 description 6
- 230000000052 comparative effect Effects 0.000 description 5
- 239000011651 chromium Substances 0.000 description 4
- 108020004465 16S ribosomal RNA Proteins 0.000 description 3
- 244000005700 microbiome Species 0.000 description 3
- 239000010802 sludge Substances 0.000 description 3
- 229920001817 Agar Polymers 0.000 description 2
- 239000008272 agar Substances 0.000 description 2
- 238000004458 analytical method Methods 0.000 description 2
- 238000012512 characterization method Methods 0.000 description 2
- 230000000694 effects Effects 0.000 description 2
- 230000007613 environmental effect Effects 0.000 description 2
- 238000005259 measurement Methods 0.000 description 2
- 238000004062 sedimentation Methods 0.000 description 2
- 239000010865 sewage Substances 0.000 description 2
- 239000007787 solid Substances 0.000 description 2
- 239000000126 substance Substances 0.000 description 2
- 238000004065 wastewater treatment Methods 0.000 description 2
- 241000894006 Bacteria Species 0.000 description 1
- 150000001448 anilines Chemical class 0.000 description 1
- 230000009286 beneficial effect Effects 0.000 description 1
- 239000000440 bentonite Substances 0.000 description 1
- 229910000278 bentonite Inorganic materials 0.000 description 1
- SVPXDRXYRYOSEX-UHFFFAOYSA-N bentoquatam Chemical compound O.O=[Si]=O.O=[Al]O[Al]=O SVPXDRXYRYOSEX-UHFFFAOYSA-N 0.000 description 1
- HFACYLZERDEVSX-UHFFFAOYSA-N benzidine Chemical compound C1=CC(N)=CC=C1C1=CC=C(N)C=C1 HFACYLZERDEVSX-UHFFFAOYSA-N 0.000 description 1
- 230000033228 biological regulation Effects 0.000 description 1
- 230000000593 degrading effect Effects 0.000 description 1
- 239000000975 dye Substances 0.000 description 1
- 238000002474 experimental method Methods 0.000 description 1
- 230000002538 fungal effect Effects 0.000 description 1
- 230000002068 genetic effect Effects 0.000 description 1
- 230000003301 hydrolyzing effect Effects 0.000 description 1
- 230000001788 irregular Effects 0.000 description 1
- 238000002955 isolation Methods 0.000 description 1
- 238000004519 manufacturing process Methods 0.000 description 1
- 238000012986 modification Methods 0.000 description 1
- 230000004048 modification Effects 0.000 description 1
- 150000002790 naphthalenes Chemical class 0.000 description 1
- 230000003204 osmotic effect Effects 0.000 description 1
- 230000020477 pH reduction Effects 0.000 description 1
- 125000001997 phenyl group Chemical group [H]C1=C([H])C([H])=C(*)C([H])=C1[H] 0.000 description 1
- 108090000623 proteins and genes Proteins 0.000 description 1
- 238000000926 separation method Methods 0.000 description 1
- 239000002689 soil Substances 0.000 description 1
- 238000003911 water pollution Methods 0.000 description 1
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- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N1/00—Microorganisms, e.g. protozoa; Compositions thereof; Processes of propagating, maintaining or preserving microorganisms or compositions thereof; Processes of preparing or isolating a composition containing a microorganism; Culture media therefor
- C12N1/20—Bacteria; Culture media therefor
- C12N1/205—Bacterial isolates
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- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12R—INDEXING SCHEME ASSOCIATED WITH SUBCLASSES C12C - C12Q, RELATING TO MICROORGANISMS
- C12R2001/00—Microorganisms ; Processes using microorganisms
- C12R2001/01—Bacteria or Actinomycetales ; using bacteria or Actinomycetales
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- C—CHEMISTRY; METALLURGY
- C02—TREATMENT OF WATER, WASTE WATER, SEWAGE, OR SLUDGE
- C02F—TREATMENT OF WATER, WASTE WATER, SEWAGE, OR SLUDGE
- C02F3/00—Biological treatment of water, waste water, or sewage
- C02F3/34—Biological treatment of water, waste water, or sewage characterised by the microorganisms used
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- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
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- C12N1/00—Microorganisms, e.g. protozoa; Compositions thereof; Processes of propagating, maintaining or preserving microorganisms or compositions thereof; Processes of preparing or isolating a composition containing a microorganism; Culture media therefor
- C12N1/20—Bacteria; Culture media therefor
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- C02—TREATMENT OF WATER, WASTE WATER, SEWAGE, OR SLUDGE
- C02F—TREATMENT OF WATER, WASTE WATER, SEWAGE, OR SLUDGE
- C02F2101/00—Nature of the contaminant
- C02F2101/10—Inorganic compounds
- C02F2101/16—Nitrogen compounds, e.g. ammonia
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- C—CHEMISTRY; METALLURGY
- C02—TREATMENT OF WATER, WASTE WATER, SEWAGE, OR SLUDGE
- C02F—TREATMENT OF WATER, WASTE WATER, SEWAGE, OR SLUDGE
- C02F2101/00—Nature of the contaminant
- C02F2101/10—Inorganic compounds
- C02F2101/20—Heavy metals or heavy metal compounds
- C02F2101/22—Chromium or chromium compounds, e.g. chromates
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Abstract
The invention discloses acinetobacter calcoaceticus and application thereof. The strain is named acinetobacter calcoaceticus FL04, is preserved in China Center for Type Culture Collection (CCTCC) with the preservation number of M2019461. The invention provides an application of acinetobacter calcoaceticus in the treatment of printing and dyeing wastewater containing benzene series. The acinetobacter calcoaceticus FL04 can effectively control and reduce the concentrations of chromium metal COD and aniline in the wastewater, and provides a new microbial treatment method for realizing low-cost treatment and standard discharge of printing and dyeing wastewater.
Description
Technical Field
The invention belongs to the technical field of environmental biology, and particularly relates to acinetobacter calcoaceticus and application thereof.
Background
Printing and dyeing are an important industrial field in China and an industry with serious pollution. With the national emphasis on environmental protection and the strict regulation of water pollution, the treatment requirements of printing and dyeing wastewater are higher and higher. Because the printing and dyeing industry generally adopts dyes containing benzene ring structures, printing and dyeing wastewater often contains a large amount of benzene series, aniline, naphthalene series and the like, so that the biochemical treatment effect of wastewater treatment is poor, and the treatment cost is increased. High-concentration microbial agents are prepared by screening high-efficiency benzene-series degrading microorganisms and added into a wastewater biochemical treatment unit, so that the treatment effect of the printing and dyeing wastewater can be improved, and the low-cost treatment and standard-reaching discharge of the printing and dyeing wastewater are realized.
Disclosure of Invention
The invention aims to provide acinetobacter calcoaceticus and application thereof. The technical scheme provided by the invention is as follows:
the acinetobacter calcoaceticus is characterized in that the strain is named acinetobacter calcoaceticus FL04 and is preserved in China Center for Type Culture Collection (CCTCC) with the preservation number of M2019461.
The invention provides an application of acinetobacter calcoaceticus in the treatment of printing and dyeing wastewater containing benzene series.
The technical scheme provided by the invention at least comprises the following beneficial effects:
1. the invention obtains the acinetobacter calcoaceticus FL04 by screening.
2. The acinetobacter calcoaceticus FL04 provided by the invention can effectively control and reduce the concentrations of chromium metal COD and aniline in the wastewater, and provides a new microbial treatment method for realizing low-cost treatment and standard discharge of printing and dyeing wastewater.
Additional advantages, objects, and features of the invention will be set forth in part in the description which follows and in part will become apparent to those having ordinary skill in the art upon examination of the following or may be learned from practice of the invention.
The preservation date of the strain is 2019, 6 and 18 months, and the preservation number is CCTCC NO. M2019461. The classification name is Acinetobacter calcoaceticus FL04(Acinetobacter calcoaceticus FL04), the name of the preservation unit is Chinese type culture preservation center, and the address is Wuhan university in Wuhan city, Hubei province, China, zip code: 430072.
drawings
FIG. 1 is a phylogenetic tree of A.calcoaceticus FL04 based on the 16S rDNA sequence.
Detailed Description
In order to make the objects, technical solutions and advantages of the present invention more apparent, the present invention will be described in further detail below. It is to be understood that the described embodiments are merely exemplary of the invention, and not restrictive of the full scope of the invention. All other embodiments, which can be obtained by a person skilled in the art without any inventive step based on the embodiments of the present invention, are within the scope of the present invention.
Isolation and characterization of A.calcoaceticus FL04
Experimental wastewater and sludge: secondary sedimentation tank sludge of sewage treatment plant of Makou town of Hanchuan city, Hubei province, and water outlet of printing and dyeing mill.
Enrichment culture medium: 1g/L phthalic acid, 0.1g/L benzidine and the balance of water, pH7.0
Screening a culture medium: 1g/L phthalic acid, 0.1g/L aniline, 0.5g/L yeast extract powder, 0.2g/L MgSO47H2O,0.1g/L CaCl2，1g/L(NH4)2SO4，1g/L K2HPO4，0.1g/L NaCl，0.02g/L FeCl3The balance being water, pH 7.0;
solid screening culture medium: 1g/L phthalic acid, 0.1g/L aniline, 0.5g/L yeast extract powder, 0.2g/L MgSO47H2O,0.1g/L CaCl2,1g/L(NH4)2SO4,1g/L K2HPO4,0.1g/L NaCl,0.02g/L FeCl315g/L agar powder and the balance of water, and the pH value is 7.0;
strain separation:
adding 90mL of enrichment medium into a 250mL triangular flask, then respectively adding sludge in a secondary sedimentation tank of a sewage treatment plant and 10g of soil near a water outlet of a printing and dyeing mill, sealing, placing on a shaking table at 37 ℃, and performing shaking culture at 150rpm for 24 hours to obtain an enrichment culture solution;
and (3) putting 100mL of screening culture medium into a 250mL triangular flask, respectively adding 1mL of enrichment culture solution, sealing, placing on a 37 ℃ shaking table, and performing shake culture at 150rpm for 48h to obtain a target culture. After being diluted in a gradient way, the target culture is coated on a solid screening culture medium (liquid screening culture medium +15g/L agar powder) plate, is kept stand and cultured for 48 hours at 37 ℃, and a single colony is picked, so that the required bacterial strain can be obtained.
Strain characterization and identification:
the colonies are semitransparent white on an LB culture medium, have smooth edges, are irregular and round, are glossy and are easy to pick; the thallus is rod-shaped, gram-negative bacteria, the optimal production pH is 6.8, the optimal growth temperature is 34-37 ℃, and the optimal osmotic pressure (NaCl%) concentration is 1.2%.
The selected single colony is preserved and has the 16S rDNA (sequence is provided) gene sequence length of 1466bp, and the 16S rDNA of the acinetobacter calcoaceticus FL04 is compared with a GenBank database by a BLAST analysis method, so that the genetic relationship between the acinetobacter calcoaceticus KW3 and the acinetobacter calcoaceticus is the closest, and the homology is as high as 99%. Phylogenetic evolution analysis was performed on both of them using MEGA program and phylogenetic trees were drawn as shown in FIG. 1. The strain is named acinetobacter calcoaceticus FL04 with a deposit number (M2019461).
Application of acinetobacter calcoaceticus FL04 in treatment of benzene-containing printing and dyeing wastewater
Taking the effluent from the hydrolytic acidification tank of the printing and dyeing mill (CODCr is 800 +/-10.2 mg/L), putting the effluent into 2000mL triangular bottles, wherein each bottle contains 1000mL, and inoculating the Acinetobacter calcoaceticus powder (the concentration of viable count of the powder)Is 2 x 109cfu/g) of the component(s) added to the printing and dyeing wastewater to a final concentration of not less than 1X 106cfu/mL (i.e., 0.5g of fungal powder per liter of wastewater), the control group was not inoculated with microorganisms and was treated with Acinetobacter calcoaceticus NCIMB9871 from Shanghai Biotech, Inc. In the treatment process, small aeration equipment is used for aeration, so that the dissolved oxygen in the water is not less than 2 mg/L. And (3) performing parallel three groups of experiments in each group, and determining the CODCr and the aniline content of the wastewater after culturing for 72 hours.
Specifically, the acinetobacter calcoaceticus FL04 powder used in the experimental group is obtained by the following method: inoculating acinetobacter calcoaceticus FL04 into a fermentation culture medium, sealing, placing in a shaking table at 30-37 ℃, performing shake culture for 40-48h to obtain acinetobacter calcoaceticus FL04 bacterial liquid, adding bentonite as a carrier, and performing spray drying to obtain acinetobacter calcoaceticus FL04 bacterial powder.
Specifically, the fermentation culture comprises 10g/L beef extract, 0.5g/L yeast extract powder, and 0.2g/LMgSO47H2O、0.1g/L CaCl2、1g/L(NH4)2SO4、1g/L K2HPO4，0.1g/L NaCl，0.02g/L FeCl3The balance of water, and the pH value is 6.8-7.0.
Specifically, the printing and dyeing wastewater is added with the initial concentration, the culture temperature and the oxygen concentration after aeration of the Acinetobacter calcoaceticus FL04 bacterial liquid, and the COD (chemical oxygen demand) in the wastewater treated by the Acinetobacter calcoaceticus FL04CrAnd aniline concentrations are shown in Table 1, to give examples 1-6 and comparative examples 1-2. COD of dyeing wastewater before treatmentCr800 +/-10.2 mg/L, 0.88 +/-0.02 mg/L of aniline substance, and 1.15 multiplied by 10 for the initial concentration of the treated acinetobacter calcoaceticus FL04 bacterial liquid of comparative example 1 provided by the invention5cfu/mL, comparative example 2 used Acinetobacter calcoaceticus NCIMB9871, supplied by Shanghai Biotech, Inc. Comparative example 3 no microorganism was added.
Finally, the COD in the treated wastewater is investigatedCrAnd aniline concentration, CODCrThe measurement of (2) is carried out by the method of HJ828-2017, and the measurement of anilines in water is carried out by GB/T11889-1989.
The results in Table 1 show that by selecting the acinetobacter calcoaceticus FL04 provided by the invention, the culture time of wastewater treatment and the oxygen concentration after aeration are controlled, and the concentrations of chromium metal COD and aniline in wastewater can be effectively controlled and reduced. Example 4 is the best for treating printing and dyeing wastewater relative to the comparative example.
TABLE 1 treatment results of dyeing wastewater
The above description is only for the purpose of illustrating the preferred embodiments of the present invention and is not to be construed as limiting the invention, and any modifications, equivalents, improvements and the like that fall within the spirit and principle of the present invention are intended to be included therein.
Sequence listing
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gccatgccgc gtgtgtgaag aaggccttat ggttgtaaag cactttaagc gaggaggagg 420
ctactgaagt taataccttc cgatagtgga cgttactcgc agaataagca ccggctaact 480
ctgtgccagc agccgcggta atacagaggg tgcaagcgtt aatcggatta actgggcgta 540
aagcgcgcgt aggcggctaa ttaagtcaaa tgtgaaatcc ccgagcttaa cttgggaatt 600
gcattcgata ctggttagct agagtgtagg agaggatggt agaattccag gtgtagcggt 660
gaaatgcgta gagatctgga ggaataccga tggcgaaggc agccatctgg cctaacactg 720
acgctgaggt gcgaaagcat ggggagcaaa caggattaga taccctggta gtccatgccg 780
taaacgatct ctactagccg ttggggcctt tgaggcttta gtggcgcagc taacgcgata 840
agtagaccgc ctggggagta cggtcgcaag actaaaactc aaatgaattg acgggggccc 900
gcataagcgg tggagcatgt ggtttaattc gatgcgacgc gaagaacctt acctggcctt 960
gacatagtaa ggactttcca gagatggatt ggtgccttcg ggaacttaca tacaggtgct 1020
gcatggctgt cgtcagctcg tgtcgtgaga tgttgggtta agtcccgcaa cgagcgcaac 1080
ccttttcctt atttgccagc gagtaatgtc gggaacttta aggatactgc cagtgacaaa 1140
ctggaggaag gcggggacga cgtcaagtca tcatggccct tacggccagg gctacacacg 1200
tgctacaatg gtcggtacaa agggttgcta cctagcgata ggatgctaat ctcaaaaagc 1260
cgatcgtagt ccggattgga gtctgcaact cgactccatg aagtcggaat cggtagtaat 1320
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catgggagtt tgttgcacca gaagtaggta gtctaaccgc aaggaggacg cttaccacga 1440
tgtggccgat gactggggtg ggctca 1466
Claims (8)
1. The acinetobacter calcoaceticus is characterized in that the strain is named acinetobacter calcoaceticus FL04 and is preserved in China Center for Type Culture Collection (CCTCC) with the preservation number of M2019461.
2. Use of acinetobacter calcoaceticus FL04 according to claim 1 for the treatment of printing and dyeing wastewater containing benzene series.
3. The use according to claim 2, wherein the acinetobacter calcoaceticus FL04 is used for reducing chromium metal COD and aniline species concentrations in wastewater.
4. The application according to claim 2, wherein the method of application is: mixing the acinetobacter calcoaceticus FL04 powder in the treatment process of the benzene-containing series printing and dyeing wastewater, aerating, adjusting the pH value to 4-6, and culturing at 30-37 ℃ for 72 h.
5. The use according to claim 2, wherein the Acinetobacter calcoaceticus FL04 bacterial powder is obtained by the following method: inoculating the acinetobacter calcoaceticus FL04 into a fermentation culture medium, sealing, placing in a shaking table at 30-37 ℃, performing shake culture for 40-48h to obtain the acinetobacter calcoaceticus FL04 bacterial liquid, adding a protective carrier, and performing spray drying to obtain bacterial powder.
6. Use according to claim 5, wherein the fermentation medium comprises beef extract, yeast extract, MgSO47H2O、CaCl2、(NH4)2SO4、K2HPO4、NaCl、FeCl3The balance of water, and the pH value is 6.8-7.0.
7. The use according to any one of claims 2 to 6, wherein the concentration of the Acinetobacter calcoaceticus FL04 after being added into the benzene-containing printing and dyeing wastewater is not less than 1 x 106cfu/mL, and the dissolved oxygen in the water is not less than 2mg/L after aeration.
8. An agent, comprising a fermentation broth obtained by fermenting the acinetobacter calcoaceticus FL04 strain of claim 1, or a dry powder agent obtained by drying the fermentation broth.
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| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN111961633A (en) * | 2020-09-09 | 2020-11-20 | 闽江学院 | Acinetobacter with hexavalent chromium removal and decoloration functions, separation and purification method and application |
| CN114196596A (en) * | 2021-12-30 | 2022-03-18 | 广东中微环保生物科技有限公司 | Cr (VI) removing composite microbial inoculum and preparation method thereof |
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| WO2013188844A2 (en) * | 2012-06-15 | 2013-12-19 | Microvi Biotech Inc. | Bioconversion processes and apparatus |
| CN104619652A (en) * | 2012-06-15 | 2015-05-13 | 微视生物技术有限公司 | Novel biocatalyst compositions and processes for use |
| CN107922915A (en) * | 2015-08-17 | 2018-04-17 | 懿科生物科技（上海）有限公司 | A kind of efficiently degrading aniline bacteria preparation and its preparation and application |
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| CN111961633A (en) * | 2020-09-09 | 2020-11-20 | 闽江学院 | Acinetobacter with hexavalent chromium removal and decoloration functions, separation and purification method and application |
| CN114196596A (en) * | 2021-12-30 | 2022-03-18 | 广东中微环保生物科技有限公司 | Cr (VI) removing composite microbial inoculum and preparation method thereof |
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