CN113249254A - Pseudomonas nitroreducens strain and application thereof - Google Patents
Pseudomonas nitroreducens strain and application thereof Download PDFInfo
- Publication number
- CN113249254A CN113249254A CN202110523456.6A CN202110523456A CN113249254A CN 113249254 A CN113249254 A CN 113249254A CN 202110523456 A CN202110523456 A CN 202110523456A CN 113249254 A CN113249254 A CN 113249254A
- Authority
- CN
- China
- Prior art keywords
- pseudomonas nitroreducens
- strain
- pseudomonas
- nitroreducens
- nitrogen
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Pending
Links
Images
Classifications
-
- C—CHEMISTRY; METALLURGY
- C02—TREATMENT OF WATER, WASTE WATER, SEWAGE, OR SLUDGE
- C02F—TREATMENT OF WATER, WASTE WATER, SEWAGE, OR SLUDGE
- C02F3/00—Biological treatment of water, waste water, or sewage
- C02F3/30—Aerobic and anaerobic processes
- C02F3/302—Nitrification and denitrification treatment
- C02F3/303—Nitrification and denitrification treatment characterised by the nitrification
-
- C—CHEMISTRY; METALLURGY
- C02—TREATMENT OF WATER, WASTE WATER, SEWAGE, OR SLUDGE
- C02F—TREATMENT OF WATER, WASTE WATER, SEWAGE, OR SLUDGE
- C02F3/00—Biological treatment of water, waste water, or sewage
- C02F3/30—Aerobic and anaerobic processes
- C02F3/302—Nitrification and denitrification treatment
- C02F3/305—Nitrification and denitrification treatment characterised by the denitrification
-
- C—CHEMISTRY; METALLURGY
- C02—TREATMENT OF WATER, WASTE WATER, SEWAGE, OR SLUDGE
- C02F—TREATMENT OF WATER, WASTE WATER, SEWAGE, OR SLUDGE
- C02F3/00—Biological treatment of water, waste water, or sewage
- C02F3/34—Biological treatment of water, waste water, or sewage characterised by the microorganisms used
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N1/00—Microorganisms, e.g. protozoa; Compositions thereof; Processes of propagating, maintaining or preserving microorganisms or compositions thereof; Processes of preparing or isolating a composition containing a microorganism; Culture media therefor
- C12N1/20—Bacteria; Culture media therefor
-
- C—CHEMISTRY; METALLURGY
- C02—TREATMENT OF WATER, WASTE WATER, SEWAGE, OR SLUDGE
- C02F—TREATMENT OF WATER, WASTE WATER, SEWAGE, OR SLUDGE
- C02F2101/00—Nature of the contaminant
- C02F2101/10—Inorganic compounds
- C02F2101/16—Nitrogen compounds, e.g. ammonia
-
- C—CHEMISTRY; METALLURGY
- C02—TREATMENT OF WATER, WASTE WATER, SEWAGE, OR SLUDGE
- C02F—TREATMENT OF WATER, WASTE WATER, SEWAGE, OR SLUDGE
- C02F2101/00—Nature of the contaminant
- C02F2101/10—Inorganic compounds
- C02F2101/16—Nitrogen compounds, e.g. ammonia
- C02F2101/163—Nitrates
Landscapes
- Life Sciences & Earth Sciences (AREA)
- Engineering & Computer Science (AREA)
- Chemical & Material Sciences (AREA)
- Microbiology (AREA)
- Organic Chemistry (AREA)
- Biodiversity & Conservation Biology (AREA)
- Hydrology & Water Resources (AREA)
- Environmental & Geological Engineering (AREA)
- Water Supply & Treatment (AREA)
- Health & Medical Sciences (AREA)
- Zoology (AREA)
- Wood Science & Technology (AREA)
- Biotechnology (AREA)
- Genetics & Genomics (AREA)
- Bioinformatics & Cheminformatics (AREA)
- Biomedical Technology (AREA)
- Virology (AREA)
- Medicinal Chemistry (AREA)
- Tropical Medicine & Parasitology (AREA)
- Biochemistry (AREA)
- General Engineering & Computer Science (AREA)
- General Health & Medical Sciences (AREA)
- Purification Treatments By Anaerobic Or Anaerobic And Aerobic Bacteria Or Animals (AREA)
- Micro-Organisms Or Cultivation Processes Thereof (AREA)
Abstract
A nitroreduction Pseudomonas strain and application thereof are disclosed, wherein the strain is nitroreduction Pseudomonas (Pseudomonas nitroreducens) pf2 which is preserved in China center for type culture Collection with the preservation number of CCTCC NO: m2021274. The strain can synchronously carry out nitrification and denitrification by taking organic carbon as a carbon source under aerobic conditions, and is suitable for removing NH from organic wastewater containing ammonia nitrogen or nitrate4 +The removal rate of-N can reach 92.7 percent, and NO3 ‑The N removal rate can reach 100 percent; and greatly simplifying the treatment process and improving the treatment effectAnd the operation and maintenance cost is reduced.
Description
Technical Field
The invention belongs to the technical field of environmental protection, and particularly relates to a pseudomonas nitroreducens strain with heterotrophic nitrification and aerobic denitrification functions and application thereof.
Background
Nitrogen pollution of water bodies becomes the most serious environmental problem in the world, and biological denitrification is the most economical and effective disposal method in water pollution treatment. The traditional biological denitrification mainly comprises nitrification and denitrification. The biological nitrification and denitrification processes are interdependent, but because of the great difference in physiology and biochemistry between nitrifying and denitrifying microorganisms, there are different tolerances and manifestations to Dissolved Oxygen (DO), temperature, pH, growth rate, Chemical Oxygen Demand (COD), and nitrates and nitrites. This makes it difficult to balance and coordinate the conventional nitrification and denitrification processes, resulting in a very complicated denitrification process and low efficiency. In recent years, many microorganisms having heterotrophic nitrification-aerobic denitrification (HN-AD) functions have been screened and applied to biological denitrification systems. HN-AD bacteria have higher cell growth rate than the traditional nitrifying microorganisms, and can utilize an organic substrate as a carbon source and simultaneously treat nitrogen pollutants with different forms, such as ammonia Nitrogen (NH)4 +-N), nitrate Nitrogen (NO)3 --N) and nitrous Nitrogen (NO)2 --N) into a nitrogen-containing gas.
Disclosure of Invention
The invention provides a nitroreduction pseudomonas strain for enriching a denitrification strain resource library, wherein the nitroreduction pseudomonas strain is a heterotrophic nitrification-aerobic denitrification strain, can enable nitrification and denitrification to be carried out simultaneously, and has great application value in the aspect of sewage treatment.
The technical scheme of the invention is as follows:
a nitroreduction Pseudomonas strain is a nitroreduction Pseudomonas (Pseudomonas nitroreducens) pf2, which is preserved in China Center for Type Culture Collection (CCTCC) at 3 months and 25 days 2021, and the preservation number is CCTCC NO: m2021274; the address is as follows: wuhan university school of eight-channel 299 # in Wuchang area of Wuhan city, Hubei province.
In a further embodiment, the effective sequence length of the 16SrDNA of Pseudomonas nitroreducens (Pseudomonas nitroreducens) pf2 is 1498bp, and the sequence is shown as SEQ ID NO: 1 is shown.
In a further scheme, the Pseudomonas nitroreducens (Pseudomonas nitroreducens) pf2 is obtained by enrichment culture, separation and purification of bottom mud of an aquaculture pond.
In a further scheme, the Pseudomonas nitroreducens (Pseudomonas nitroreducens) pf2 is a gram-negative bacillus, is in a short rod shape, has neat edges and has milky colony.
In a further embodiment, the Pseudomonas nitroreducens pf2 is grown using ammonium sulfate or nitrate as the sole nitrogen source.
Another object of the invention is to provide an application of the Pseudomonas nitroreducens strain, wherein pf2 is used for nitrogen removal of nitrogen-containing water.
In a further scheme, the nitrogen-containing water body refers to organic wastewater containing ammonia nitrogen and nitrate.
In a further scheme, the Pseudomonas nitroreducens (Pseudomonas nitroreducens) pf2 is inoculated in a nitrogen-containing water body for aerobic continuous culture after being subjected to expanded culture.
In a further scheme, the temperature of the aerobic continuous culture is 30-35 ℃; the inoculum size of the Pseudomonas nitroreducens pf2 was 1% (volume by volume).
The effective sequence length of the 16SrDNA of the nitroreduction Pseudomonas (Pseudomonas nitroreducens) pf2 is 1498bp, and the sequence is shown as SEQ ID NO: 1 is shown. The strain was identified to belong to the genus Pseudomonas (Pseudomonas sp.) by BLAST sequence homology alignment and was designated as Pseudomonas nitroreducens (pf 2).
The technical scheme of the invention has the following advantages:
1. the Pseudomonas nitroreducens pf2 is a heterotrophic nitrification-aerobic denitrification strain, namely, the Pseudomonas nitroreducens (Pseudomonas nitroreducens) can synchronously carry out nitrification and denitrification by taking organic carbon as a carbon source under aerobic conditions, and is suitable for removing organic wastewater containing ammonia nitrogen or nitrate.
2. Compared with the traditional denitrification technology, the invention adopts Pseudomonas nitroreducens (pf 2) for denitrification of the nitrogen-containing water body, thereby greatly simplifying the treatment process, improving the treatment efficiency and reducing the operation and maintenance cost.
3. The Pseudomonas nitroreducens (Pseudomonas nitroreducens) pf2 has a remarkable ammonia nitrogen removal effect. The nitroreduction pseudomonas pf2 is added into ammonia nitrogen wastewater with the initial concentration of 175mg/L, the temperature is 30 ℃, the rotating speed is 220rpm, the TN removal rate can reach 100 percent, and the NH content is4 +The removal rate of-N can reach 92.7%, the accumulation of intermediate products is less, and the ammonia nitrogen removal effect is good.
4. The strain pf2 has aerobic denitrification capability: in nitrate wastewater with the initial concentration of 100mg/L, the temperature is 30 ℃, the rotating speed is 220rpm, the TN removal rate can reach 95.5 percent, and NO is3 -The removal rate of-N can reach 100 percent, and the aerobic denitrification capability is obvious.
Drawings
The biological denitrification of Pseudomonas nitroreducens pf2 will be illustrated by the drawings in the present application.
FIG. 1 is a 16S rDNA phylogenetic clade of Pseudomonas nitroreducens pf 2;
FIG. 2 shows the growth characteristics of said Pseudomonas nitroreducens with ammoniacal nitrogen and nitrate nitrogen as the sole nitrogen source;
FIG. 3 shows the denitrification capacity of Pseudomonas nitroreducens pf2 in ammonia nitrogen wastewater with an initial concentration of 175 mg/L;
FIG. 4 is a denitrification performance analysis of Pseudomonas nitroreducens pf2 using nitrate as a nitrogen source.
Detailed Description
The present invention will be described in further detail with reference to the following examples and the accompanying drawings.
The methods for measuring total nitrogen, ammonia nitrogen, nitrate nitrogen and nitrite nitrogen referred to in the following examples are as follows:
the total nitrogen is measured by alkaline potassium persulfate digestion ultraviolet spectrophotometry (GB 11894-89);
ammonia nitrogen is measured by adopting a method for measuring the ammonia nitrogen in water by adopting a nano reagent spectrophotometry (HJ 535-2009);
nitrate Nitrogen (NO)3 --N) respectively adopting a method (HZ-HJ-SZ-0138) for measuring nitrate nitrogen by adopting ultraviolet spectrophotometry water quality;
nitrous acid Nitrogen (NO)2 -The measurement of-N) was carried out by using naphthyl ethylenediamine hydrochloride spectrophotometry.
Example 1:
a nitroreduction Pseudomonas strain is a nitroreduction Pseudomonas (Pseudomonas nitroreducens) pf2, which is preserved in China Center for Type Culture Collection (CCTCC) at 3 months and 25 days 2021, with the preservation numbers as follows: CCTCC NO: m2021274; the address is as follows: wuhan university school of eight-channel 299 # in Wuchang area of Wuhan city, Hubei province.
1. Enrichment and isolation of strains
1.1 evenly mixing and standing part of bottom mud collected from the culture pond by using normal saline, and taking supernatant as a sample dilution stock solution. Adding the diluted stock solution into a heterotrophic nitrification enrichment medium according to the volume ratio of 10 percent, and performing shake culture at the temperature of 30 ℃ and the rpm/min for 3 days.
Wherein, the heterotrophic nitrification enrichment culture medium: will be (NH)4)2SO40.472g, 4.052g of sodium succinate and 50ml of Vickers salt solution, adding water for dissolution, supplementing distilled water to 1L, and adjusting the pH to 7.0. Wherein the solute in the Vickers salt solution (g/L) is composed of the following raw materials: k2HPO4 5.0g，FeSO4·7H2O 0.05g，NaCl 2.5g，MgSO4·7H2O 2.5g，MnSO4·4H2O 0.05g。
1.2 inoculating the domesticated microorganisms into an aerobic denitrification culture medium for further screening, wherein the aerobic denitrification culture medium takes potassium nitrate as a unique nitrogen source.
Aerobic denitrification culture medium: 0.36g/L KNO3，10.55g/L Na2HPO4·12H2O，1.5g/L KH2PO4， 0.1g/L MgSO4·7H2O, 4.0g/L sodium citrate and 0.2 percent (volume ratio) of trace element solution. Wherein the trace element solution: 50g/L EDTA-Na2, 2.2g/L ZnSO4，5.5g/L CaCl2，5.06g/L MnCl2·4H2O， 5.0g/L FeSO4·7H2O，1.57g/L CuSO4·5H2O，1.61g/L CoCl2·6H2O。
1.3 taking 1mL of sample which is subjected to primary screening by the aerobic denitrification culture medium, uniformly coating the sample on a solid GN chromogenic culture medium, and then placing the sample in a constant temperature incubator at 30 ℃ for culture to obtain bacterial colonies.
1.4 to further verify that the single colony picked has the property of changing the GN color medium to blue, the single colony which is blue in the solid GN color medium was picked and inoculated into 3mL of sterilized liquid GN color medium, shaking cultured at 30 ℃ and 150r/min, the color change was observed, and a strain which can change the liquid GN color medium from green to blue was selected as a rescreened strain.
Wherein the liquid GN chromogenic medium consists of: 1.0g/L KNO38.5g/L sodium citrate, 1.0g/L L-asparagine, 1.0g/L KH2PO4，1.0g/L MgSO4·7H2O，0.2g/L CaCl2·6H2O，0.05g/L FeCl3·6H2O, 0.1% (by volume) bromothymol blue (BTB) solution, and the pH was adjusted to 7.0.
Adding agar 2% to the liquid chromogenic medium to prepare solid chromogenic medium, and sterilizing at 121 deg.C for 20 min.
2. Molecular biological identification
Selecting single colony of one of the rescreened bacteria for 16S rRNA gene amplification, and performing PC by using common primers 27F (5'-AGAGTTTGATCCTGGCTCAG-3') and 1492R (5 '-TACGGYTACCTTGTTACGACTT-3') for 16S rRNA geneAnd (3) amplifying R. Wherein the 50 mu L PCR reaction system comprises: 2 XTaq PCR Master Mix 25. mu.L, 27F (10. mu.M) 1. mu.L, 1492R (10. mu.M) 1. mu.L, DNA template as single colony picked, ddH2O24. mu.L. Setting a PCR program: pre-denaturation at 95 ℃ for 5 min; denaturation at 95 ℃ for 30s, annealing at 55 ℃ for 45s, and extension at 72 ℃ for 40s, and amplification for 30 cycles; final extension at 72 ℃ for 10 min. The 16S rDNA Sequence obtained by sequencing was submitted to Genbank database (https:// blast.ncbi.nlm.nih.gov/blast.cgi) for Sequence alignment, the database used was 16S ribosome RNAAdatabes, and the most similar species to the target strain was determined according to the highest Sequence coverage (Sequence coverage) and similarity (Identity).
The effective sequence length of the sequenced 16SrDNA is 1498bp, and the sequence is shown as SEQ ID NO: 1 is shown. The genetic relationship between the strain and other strains is shown in figure 1, phylogenetic analysis confirms that the 16S rDNA sequence of the bacterium has more than 99 percent of homology with nitroreduction Pseudomonas (Pseudomonas nitroreducens) published in NCBI database, so that the bacterium is confirmed to be nitroreduction Pseudomonas and named as nitroreduction Pseudomonas pf2, the strain is sent to China center for type culture collection for preservation, and the preservation numbers are as follows: CCTCC NO: m2021274; the preservation date is as follows: 3 months and 25 days 2021; the address is as follows: wuhan university school of eight-channel 299 # in Wuchang area of Wuhan city, Hubei province.
Example 2, the removal capacity of pseudomonas nitroreducens pf2 on ammonia nitrogen and total nitrogen in wastewater.
By NH4 +N is nitrogen source, sodium citrate is carbon source, NH is in waste water4 +The growth and ammonia nitrogen and total nitrogen removal capacity of strain pf2 were examined at an N concentration of 175 mg/L.
Selecting the bacterial colony of the re-screened bacteria separated in the example 1 to a heterotrophic nitrification ammonia nitrogen culture medium (NM) and culturing for 18h at the speed of 220rpm of a 30 ℃ gas bath shaking table, and specifically comprising the following steps: taking liquid strains (2ml) according to the volume ratio of 1% of the culture medium, and inoculating the liquid strains into 200ml NM (heterotrophic nitrification ammonia nitrogen culture medium). The formula of NM culture medium is: (NH)4)2SO40.945g/L, 16.34g/L sodium citrate and MgSO4·7H2O 1g/L，KH2PO4 0.25g/L，Na2HPO4 0.3g/L。
As can be seen from FIG. 2, the strain was grown in log phase for 8h, in plateau phase for 24h, and the OD600 of the strain was raised to 1.909 for 32 h. As can be seen from FIG. 3, the removal rate of the bacterial strain to ammonia nitrogen reaches 91.1%, the removal rate to total nitrogen reaches 100%, and nitrate nitrogen and nitrite nitrogen as intermediate products accumulate less. The strain pf2 is shown to have better treatment effect on high-concentration ammonia nitrogen wastewater.
Example 3 removal of nitrate Nitrogen from wastewater by Pseudomonas nitroreducens pf2
The strain pf2 takes nitrate as a nitrogen source, and the aerobic denitrification capability of the strain is examined by detecting the degradation condition of the nitrogen source in the wastewater. Initial TN concentration of the nitrate wastewater is 147mg/L, and initial NO3 -The liquid seed culture (2ml) of the rescreened strain isolated in example 1 was inoculated into 200ml of a nitrate medium at an N concentration of 99mg/L at a medium volume ratio of 1%, and cultured at 30 ℃ and a rotation speed of 220 rpm. The formula of the culture medium is as follows: KNO30.722 g/L, sodium citrate 5g/L, MgSO4·7H2O 1g/L，KH2PO4 0.25g/L，Na2HPO40.3 g/L. As can be seen from FIG. 2, the strain pf2 was cultured for 16h, and the OD600 reached up to 1.338. As shown in FIG. 4, the removal rate of nitrate nitrogen by the strain at this time reached 100%, and the total nitrogen removal rate was 95.5%. The experimental result shows that the strain pf2 can perform aerobic denitrification by taking nitrate nitrogen as a substrate under aerobic conditions.
The above-described embodiments are merely preferred embodiments of the present invention, and the embodiments of the present invention are not limited to the above-described embodiments, and it should be understood that many other modifications and embodiments can be devised by those skilled in the art, which will fall within the spirit and scope of the principles of this disclosure.
Sequence listing
<110> aquatic research institute of agricultural science institute of Anhui province
<120> Pseudomonas nitroreducens strain and application thereof
<141> 2021-03-24
<160> 1
<170> SIPOSequenceListing 1.0
<210> 1
<211> 1498
<212> DNA
<213> Pseudomonas nitroreducens (Pseudomonas nitroreducens) pf2
<400> 1
agagtttgat cctggcttag attgaacgct ggcggcaggc ctaacacatg caagtcgagc 60
ggatgagtgg agcttgctcc atgattcagc ggcggacggg tgagtaatgc ctaggaatct 120
gcctggtagt gggggacaac gtttcgaaag gaacgctaat accgcatacg tcctacggga 180
gaaagcaggg gaccttcggg ccttgcgcta tcagatgagc ctaggtcgga ttagctagtt 240
ggtggggtaa aggcctacca aggcgacgat ccgtaactgg tctgagagga tgatcagtca 300
cactggaact gagacacggt ccagactcct acgggaggca gcagtgggga atattggaca 360
atgggcgaaa gcctgatcca gccatgccgc gtgtgtgaag aaggtcttcg gattgtaaag 420
cactttaagt tgggaggaag ggcagtaagt taataccttg ctgttttgac gttaccaaca 480
gaataagcac cggctaactt cgtgccagca gccgcggtaa tacgaagggt gcaagcgtta 540
atcggaatta ctgggcgtaa agcgcgcgta ggtggtttgg taagatggat gtgaaatccc 600
cgggctcaac ctgggaactg catccataac tgcctgacta gagtacggta gagggtggtg 660
gaatttcctg tgtagcggtg aaatgcgtag atataggaag gaacaccagt ggcgaaggcg 720
accacctgga ctgatactga cactgaggtg cgaaagcgtg gggagcaaac aggattagat 780
accctggtag tccacgccgt aaacgatgtc gactagccgt tgggatcctt gagatcttag 840
tggcgcagct aacgcgataa gtcgaccgcc tggggagtac ggccgcaagg ttaaaactca 900
aatgaattga cgggggcccg cacaagcggt ggagcatgtg gtttaattcg aagcaacgcg 960
aagaacctta cctggccttg acatgtccgg aaccttgcag agatgcgagg gtgccttcgg 1020
gaatcggaac acaggtgctg catggctgtc gtcagctcgt gtcgtgagat gttgggttaa 1080
gtcccgtaac gagcgcaacc cttgtcctta gttaccagca cgttatggtg ggcactctaa 1140
ggagactgcc ggtgacaaac cggaggaagg tggggatgac gtcaagtcat catggccctt 1200
acggccaggg ctacacacgt gctacaatgg tcggtacaga gggttgccaa gccgcgaggt 1260
ggagctaatc ccataaaacc gatcgtagtc cggatcgcag tctgcaactc gactgcgtga 1320
agtcggaatc gctagtaatc gtgaatcaga atgtcacggt gaatacgttc ccgggccttg 1380
tacacaccgc ccgtcacacc atgggagtgg gttgctccag aagtagctag tctaaccgca 1440
agggggacgg ttaccacgga gtgattcatg actggggtga agtcgtaaca aggtagcc 1498
Claims (9)
1. A nitroreduction pseudomonas strain is characterized in that: the strain is Pseudomonas nitroreducens (Pseudomonas nitroreducens) pf2, which is preserved in China center for type culture Collection with the preservation number of CCTCC NO: m2021274.
2. The pseudomonas nitroreducens strain of claim 1, wherein: the effective sequence length of the 16SrDNA of the Pseudomonas nitroreducens (Pseudomonas nitroreducens) pf2 is 1498bp, and the sequence is shown as SEQ ID NO: 1 is shown.
3. The pseudomonas nitroreducens strain of claim 1, wherein: the Pseudomonas nitroreducens (Pseudomonas nitroreducens) pf2 is obtained by enrichment culture, separation and purification of bottom mud of an aquaculture pond.
4. The pseudomonas nitroreducens strain of claim 1, wherein: the nitropseudomonas nitroreducens (pf 2) is a gram-negative bacillus, is in a short rod shape, has irregular edges and is milky white in colony.
5. The pseudomonas nitroreducens strain of claim 1, wherein: the Pseudomonas nitroreducens pf2 was grown using ammonium sulfate or nitrate as the sole nitrogen source.
6. Use of a strain of pseudomonas nitroreducens according to any one of claims 1 to 5, characterized in that: the Pseudomonas nitroreducens (Pseudomonas nitroreducens) pf2 is used for denitrification of nitrogen-containing water bodies.
7. Use according to claim 6, characterized in that: the nitrogen-containing water body refers to organic wastewater containing ammonia nitrogen or nitrate.
8. Use according to claim 6, characterized in that: the Pseudomonas nitroreducens (Pseudomonas nitroreducens) pf2 is inoculated in a nitrogen-containing water body for aerobic continuous culture after amplification culture.
9. Use according to claim 8, characterized in that: the temperature of the aerobic continuous culture is 30-35 ℃; the inoculation amount of the Pseudomonas nitroreducens pf2 is 1 percent of the volume of the nitrogen-containing water body.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN202110523456.6A CN113249254A (en) | 2021-05-13 | 2021-05-13 | Pseudomonas nitroreducens strain and application thereof |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN202110523456.6A CN113249254A (en) | 2021-05-13 | 2021-05-13 | Pseudomonas nitroreducens strain and application thereof |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| CN113249254A true CN113249254A (en) | 2021-08-13 |
Family
ID=77181752
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN202110523456.6A Pending CN113249254A (en) | 2021-05-13 | 2021-05-13 | Pseudomonas nitroreducens strain and application thereof |
Country Status (1)
| Country | Link |
|---|---|
| CN (1) | CN113249254A (en) |
Cited By (4)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN114395502A (en) * | 2021-12-02 | 2022-04-26 | 平湖石化有限责任公司 | Pseudomonas nitroreducens WX4-1 for efficiently degrading tetramethylbenzene and application thereof |
| CN114933988A (en) * | 2022-05-11 | 2022-08-23 | 江苏科技大学 | Pseudomonas with aerobic denitrification synchronous nitrogen and phosphorus removal performance |
| CN115261289A (en) * | 2022-09-26 | 2022-11-01 | 山东省科学院生态研究所(山东省科学院中日友好生物技术研究中心) | Nitro-reduction pseudomonas PNr-1, product thereof and application thereof in prevention and treatment of strawberry leaf diseases |
| CN115948288A (en) * | 2022-12-06 | 2023-04-11 | 浙江大学 | Aerobic efficient denitrification compound flora and application thereof |
Citations (7)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| US5508193A (en) * | 1993-08-31 | 1996-04-16 | Regents Of The University Of Minnesota | Pseudomonas strain for degradation of s-triazines in soil and water |
| TWI240703B (en) * | 2001-05-01 | 2005-10-01 | Sung-Yu He | Sewage processing method with microorganisms |
| CN101643716A (en) * | 2009-07-31 | 2010-02-10 | 华北电力大学 | Pseudomonas nitroreducens and application thereof |
| CN104789507A (en) * | 2015-04-27 | 2015-07-22 | 北京农学院 | Bacteria BMJHZ-01 for degrading difenoconazole and screening method thereof |
| CN107012107A (en) * | 2017-03-01 | 2017-08-04 | 北京禾和润生科技有限公司 | One nitro-reductive pseudomonads and its tunning and purposes |
| CN108102978A (en) * | 2018-02-06 | 2018-06-01 | 北京大学 | A kind of degradation bacteria strains JN8 of oily sludge petrochina hydro carbons and its application |
| KR20190012685A (en) * | 2017-07-28 | 2019-02-11 | 고려대학교 산학협력단 | Microbial agents for promoting plant growth comprising Pseudomonas nitroreducens or culture thereof as active ingredient |
-
2021
- 2021-05-13 CN CN202110523456.6A patent/CN113249254A/en active Pending
Patent Citations (7)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| US5508193A (en) * | 1993-08-31 | 1996-04-16 | Regents Of The University Of Minnesota | Pseudomonas strain for degradation of s-triazines in soil and water |
| TWI240703B (en) * | 2001-05-01 | 2005-10-01 | Sung-Yu He | Sewage processing method with microorganisms |
| CN101643716A (en) * | 2009-07-31 | 2010-02-10 | 华北电力大学 | Pseudomonas nitroreducens and application thereof |
| CN104789507A (en) * | 2015-04-27 | 2015-07-22 | 北京农学院 | Bacteria BMJHZ-01 for degrading difenoconazole and screening method thereof |
| CN107012107A (en) * | 2017-03-01 | 2017-08-04 | 北京禾和润生科技有限公司 | One nitro-reductive pseudomonads and its tunning and purposes |
| KR20190012685A (en) * | 2017-07-28 | 2019-02-11 | 고려대학교 산학협력단 | Microbial agents for promoting plant growth comprising Pseudomonas nitroreducens or culture thereof as active ingredient |
| CN108102978A (en) * | 2018-02-06 | 2018-06-01 | 北京大学 | A kind of degradation bacteria strains JN8 of oily sludge petrochina hydro carbons and its application |
Non-Patent Citations (1)
| Title |
|---|
| ZHANG DUOYING ET AL.: ""Isolation, Identification and Characterization of heterotrophic nitrifying bacteria from surface water"", 《ADVANCED MATERIALS RESEARCH》 * |
Cited By (7)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN114395502A (en) * | 2021-12-02 | 2022-04-26 | 平湖石化有限责任公司 | Pseudomonas nitroreducens WX4-1 for efficiently degrading tetramethylbenzene and application thereof |
| CN114395502B (en) * | 2021-12-02 | 2023-09-12 | 平湖石化有限责任公司 | Nitro-reduction pseudomonas WX4-1 for efficiently degrading tetramethylene and application thereof |
| CN114933988A (en) * | 2022-05-11 | 2022-08-23 | 江苏科技大学 | Pseudomonas with aerobic denitrification synchronous nitrogen and phosphorus removal performance |
| CN114933988B (en) * | 2022-05-11 | 2024-05-28 | 江苏科技大学 | Pseudomonas with aerobic denitrification synchronous denitrification and dephosphorization performance |
| CN115261289A (en) * | 2022-09-26 | 2022-11-01 | 山东省科学院生态研究所(山东省科学院中日友好生物技术研究中心) | Nitro-reduction pseudomonas PNr-1, product thereof and application thereof in prevention and treatment of strawberry leaf diseases |
| CN115948288A (en) * | 2022-12-06 | 2023-04-11 | 浙江大学 | Aerobic efficient denitrification compound flora and application thereof |
| CN115948288B (en) * | 2022-12-06 | 2023-09-26 | 浙江大学 | Aerobic high-efficiency denitrification compound flora and application thereof |
Similar Documents
| Publication | Publication Date | Title |
|---|---|---|
| CN107201325B (en) | Pseudomonas strain and culture method and application thereof | |
| CN113249254A (en) | Pseudomonas nitroreducens strain and application thereof | |
| CN102443558B (en) | Composite heterotrophic nitrifying bacterial agent and application of same in nitrogen removal treatment of waste water containing ammonia and nitrogen | |
| CN109810923B (en) | Aerobic denitrifying bacterium SLY2-21 for sewage denitrification and application thereof | |
| CN105733998B (en) | Efficient denitrification strain with heterotrophic nitrification and aerobic denitrification capabilities | |
| CN113174345A (en) | Heterotrophic nitrification-aerobic denitrification strain for efficient denitrification and application thereof | |
| CN114703095B (en) | Pseudomonas adulthood and application thereof in field of sewage and wastewater purification | |
| CN107603907B (en) | Bacillus aryabhattai GH-9 and application thereof | |
| CN104611279B (en) | A kind of red city Rhodococcus sp LH N13 and its microbial bacterial agent and purposes | |
| CN115786180A (en) | Pseudomonas stutzeri BBW831 and application thereof | |
| CN114456974B (en) | Paracoccus thiooxidans capable of efficiently degrading DMF and application thereof in DMF-containing wastewater treatment | |
| CN113234626A (en) | Strain with heterotrophic nitrification-aerobic denitrification function and application thereof | |
| CN112266885B (en) | Heterotrophic nitrification aerobic denitrifying bacteria Y16 and application thereof | |
| CN111979138B (en) | Heterotrophic nitrification aerobic denitrifying bacterium Y15 and application thereof | |
| CN116622556B (en) | Low-temperature-resistant high-alkalinity high-efficiency denitrifying bacterium, screening method and application thereof | |
| CN111471611A (en) | Rhodococcus ruber HDRR1 for purifying inorganic nitrogen and phosphorus in tail water of seawater pond culture and application thereof | |
| CN117467555A (en) | Bacillus pricklyash strain and application thereof | |
| CN111471614A (en) | Salt-tolerant strain and biodegradation method for high-salt chemical wastewater | |
| Hongyi et al. | Anammox bacterial abundance and biodiversity in greenhouse vegetable soil are influenced by high nitrate content | |
| CN113416681B (en) | Heterotrophic nitrification-aerobic denitrification bacteria resistant to low carbon and high nitrogen and application thereof | |
| CN113736700B (en) | Heterotrophic nitrification-aerobic denitrification bacteria and application thereof | |
| CN113699060B (en) | Low-carbon high-nitrogen-resistant heterotrophic nitrification-aerobic denitrification sphingosine box fungus and application thereof | |
| CN110157637A (en) | Enterobacteria Z1 and klebsiella Z2 composite bacteria agent removal high nitrogen pollutant effluents and application | |
| CN114684911A (en) | Salt-tolerant aerobic granular sludge forming method based on moderate halophilic flora and application thereof | |
| CN110468066B (en) | Aerobic denitrifying strain and application thereof |
Legal Events
| Date | Code | Title | Description |
|---|---|---|---|
| PB01 | Publication | ||
| PB01 | Publication | ||
| SE01 | Entry into force of request for substantive examination | ||
| SE01 | Entry into force of request for substantive examination | ||
| WD01 | Invention patent application deemed withdrawn after publication |
Application publication date: 20210813 |
|
| WD01 | Invention patent application deemed withdrawn after publication |