CN110791424A - Working system and working method for sample grinding - Google Patents

Working system and working method for sample grinding Download PDF

Info

Publication number
CN110791424A
CN110791424A CN201911266311.1A CN201911266311A CN110791424A CN 110791424 A CN110791424 A CN 110791424A CN 201911266311 A CN201911266311 A CN 201911266311A CN 110791424 A CN110791424 A CN 110791424A
Authority
CN
China
Prior art keywords
cooling
sample
grinding
mechanical arm
module
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Withdrawn
Application number
CN201911266311.1A
Other languages
Chinese (zh)
Inventor
谷田
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
Qingdao Strange Point Biological Co Ltd
Original Assignee
Qingdao Strange Point Biological Co Ltd
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Qingdao Strange Point Biological Co Ltd filed Critical Qingdao Strange Point Biological Co Ltd
Priority to CN201911266311.1A priority Critical patent/CN110791424A/en
Publication of CN110791424A publication Critical patent/CN110791424A/en
Withdrawn legal-status Critical Current

Links

Images

Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
    • C12M41/00—Means for regulation, monitoring, measurement or control, e.g. flow regulation
    • C12M41/48—Automatic or computerized control
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
    • C12M41/00—Means for regulation, monitoring, measurement or control, e.g. flow regulation
    • C12M41/12—Means for regulation, monitoring, measurement or control, e.g. flow regulation of temperature
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
    • C12M41/00—Means for regulation, monitoring, measurement or control, e.g. flow regulation
    • C12M41/30—Means for regulation, monitoring, measurement or control, e.g. flow regulation of concentration
    • C12M41/34—Means for regulation, monitoring, measurement or control, e.g. flow regulation of concentration of gas
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
    • C12M41/00—Means for regulation, monitoring, measurement or control, e.g. flow regulation
    • C12M41/44—Means for regulation, monitoring, measurement or control, e.g. flow regulation of volume or liquid level
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
    • C12M47/00—Means for after-treatment of the produced biomass or of the fermentation or metabolic products, e.g. storage of biomass
    • C12M47/06—Hydrolysis; Cell lysis; Extraction of intracellular or cell wall material
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
    • C12M47/00—Means for after-treatment of the produced biomass or of the fermentation or metabolic products, e.g. storage of biomass
    • C12M47/20—Heating or cooling

Landscapes

  • Chemical & Material Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Health & Medical Sciences (AREA)
  • Wood Science & Technology (AREA)
  • Zoology (AREA)
  • Organic Chemistry (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Biotechnology (AREA)
  • Genetics & Genomics (AREA)
  • Microbiology (AREA)
  • Sustainable Development (AREA)
  • Biomedical Technology (AREA)
  • Biochemistry (AREA)
  • General Engineering & Computer Science (AREA)
  • General Health & Medical Sciences (AREA)
  • Analytical Chemistry (AREA)
  • Physics & Mathematics (AREA)
  • Thermal Sciences (AREA)
  • Computer Hardware Design (AREA)
  • Cell Biology (AREA)
  • Molecular Biology (AREA)
  • Clinical Laboratory Science (AREA)
  • Sampling And Sample Adjustment (AREA)

Abstract

A control system controls a mechanical arm A to sequentially place a pipe frame and a precooling aluminum module on an operation table from full-automatic storage equipment, controls a code reader to read a code of the pipe frame and controls a mechanical arm B to clamp and place a cryopreserved pipe on the pipe frame into the precooling aluminum module, then controls a liquid nitrogen tank to open a tank opening and controls the mechanical arm A to place the precooling aluminum module into the liquid nitrogen tank for cooling, controls the mechanical arm A to place the precooling aluminum module into a diverse tissue grinder after cooling is completed, grinds a sample in the cryopreserved pipe, controls the mechanical arm A to take out the precooling aluminum module and place the precooling aluminum module on the operation table, controls the mechanical arm B to place the cryopreserved pipe into the pipe frame, and then utilizes the mechanical arm A to place the pipe frame and the precooling aluminum module into the full-automatic storage equipment. The invention realizes full automation of sample grinding, reduces the workload of workers, and improves the efficiency and quality of sample grinding by counting and analyzing the grinding data.

Description

Working system and working method for sample grinding
Technical Field
The invention relates to the fields of biology, health and medical treatment, in particular to a working system and a working method for sample grinding.
Background
In practice, many experimental materials need to be ground before their components, genes and proteins, and structure and function can be studied.
The liquid nitrogen grinding is widely applied in experiments, the temperature of the liquid nitrogen is extremely low, various tissue components are not easy to damage or degrade, the tissue is hard, the brittleness is increased, and the grinding is easy; the liquid nitrogen grinding is used for stopping all biological reactions inside and outside the sample, and the sample is completely frozen in the liquid nitrogen and can achieve a good grinding effect.
However, when the sample is put into the liquid nitrogen, a manual placing method is generally adopted, tools are needed to be used when the sample is placed and taken out, time is wasted, efficiency is not high, hands of people are easily frostbitten, suffocation risks exist, and extra care is needed when the liquid nitrogen sampling device is used.
Disclosure of Invention
The invention aims to provide a working system and a working method for grinding a sample, which have the advantages of whole-process monitoring and full-automatic sample taking for cooling and grinding.
The technical purpose of the invention is realized by the following technical scheme:
a sample polishing work system, the system being located within an enclosed environment, comprising the following components:
the full-automatic sample storage assembly comprises full-automatic storage equipment, wherein the full-automatic storage equipment is provided with a manual sample inlet and outlet port and an automatic sample inlet and outlet port, the manual sample inlet and outlet port is communicated with the outside of a closed environment, the automatic sample inlet and outlet port is communicated with the inside of the closed environment, and the full-automatic storage equipment is used for placing and conveying a pipe frame and a precooling aluminum module;
the mechanical arm A is used for clamping the pipe frame and the precooling aluminum module;
the turntable assembly comprises a mechanical arm B and an operating platform, wherein the mechanical arm B is used for clamping a frozen pipe in a pipe frame on the operating platform into the pre-cooling aluminum module;
the code reader is positioned on a running line from the automatic sample loading assembly to the turntable assembly of the mechanical arm A and is used for reading codes for the tube rack and identifying different sample types;
the liquid nitrogen cooling assembly comprises a liquid nitrogen tank and is used for cooling the pre-cooling aluminum module;
the grinding assembly comprises a tissue grinder and a pre-cooling aluminum module, wherein the tissue grinder is used for grinding a sample in a cryopreservation tube in the pre-cooling aluminum module;
and the control system is used for controlling the full-automatic sample storage assembly, the mechanical arm A, the turntable assembly, the code reader, the liquid nitrogen cooling assembly and the grinding assembly.
The specific operation steps are as follows:
step 1, respectively placing a pipe frame and a pre-cooling aluminum module into full-automatic storage equipment through a manual sample inlet and outlet of the full-automatic storage equipment, wherein a freezing tube is placed in the pipe frame, and the freezing tube contains a sample;
step 2, the control system controls the mechanical arm A to take out the pipe frame and the pre-cooling aluminum module in sequence through an automatic sample inlet and outlet of the full-automatic storage device and place the pipe frame and the pre-cooling aluminum module on an operation table, in the process of conveying the pipe frame, a code reader reads codes of the pipe frame, the type of a sample is identified, the type of the sample is transmitted to the control system through an electric signal, and the control system adjusts different cooling time and grinding time according to different types of the sample;
step 3, controlling a mechanical arm B to clamp and place the cryopreserved pipe in the pipe frame on the operating platform into the precooling aluminum module by the control system;
step 4, controlling a liquid nitrogen tank to open a tank opening by a control system, and then controlling a mechanical arm A to clamp a precooled aluminum module filled with a freezing storage pipe and placing the precooled aluminum module into the liquid nitrogen tank for cooling;
step 5, when the cooling time is up, the control system controls the mechanical arm A to clamp and place the pre-cooling aluminum module in the tissue grinder, and the sample in the cryopreservation tube in the pre-cooling aluminum module is ground;
step 6, after grinding is finished, controlling the mechanical arm A to clamp and place the precooled aluminum module on the operation table by the control system;
step 7, controlling a mechanical arm B to clamp and place the cryopreserved pipe in the pre-cooling aluminum module into a pipe frame by the control system;
and 8, controlling the mechanical arm A to respectively clamp the pipe frame and the pre-cooling aluminum module to be placed in full-automatic storage equipment through an automatic sample inlet and outlet port by the control system, and taking the pipe frame provided with the frozen pipe after grinding treatment away from the manual sample inlet and outlet port.
The preferred scheme is as follows:
preferably: the cooling time in the step 4 is 10 s-2 min. Different samples need different cooling time lengths, and the cooling time range is 10 s-2 min according to system calculation.
Preferably: the grinding frequency in the step 5 is 10-70 HZ/s, and the grinding time is 30 s-2 min. Different samples need different grinding frequencies and grinding durations, and the grinding frequency is 10-70 HZ/s and the time range is 30 s-2 min according to system calculation.
In the operation process, because the cooling pipe can be directly placed in the pre-cooling aluminum module for subsequent cooling and grinding, and the turntable assembly is not needed for carrying out turntable back cooling and grinding on the cooling pipe, the following technical scheme is provided:
a sample polishing work system, the system being located within an enclosed environment, comprising the following components:
the full-automatic sample storage assembly comprises full-automatic storage equipment, wherein the full-automatic storage equipment is provided with a manual sample inlet and outlet and an automatic sample inlet and outlet, the manual sample inlet and outlet is communicated with the outside of a closed environment, the automatic sample inlet and outlet is communicated with the inside of the closed environment, and the full-automatic storage equipment is used for placing and conveying pre-cooling aluminum modules;
the mechanical arm A is used for clamping the pre-cooled aluminum module;
the liquid nitrogen cooling assembly comprises a liquid nitrogen tank and is used for cooling the pre-cooling aluminum module;
the code reader is positioned on a running line from the full-automatic sample storage assembly to the liquid nitrogen cooling assembly of the mechanical arm A and is used for scanning codes of the precooled aluminum module and identifying different sample types;
the grinding assembly comprises a tissue grinder and a pre-cooling aluminum module, wherein the tissue grinder is used for grinding a sample in a cryopreservation tube in the pre-cooling aluminum module;
and the control system is used for controlling the full-automatic sample storage assembly, the mechanical arm A, the code reader, the liquid nitrogen cooling assembly and the grinding assembly.
The specific operation steps are as follows:
step 1, respectively placing a pre-cooling aluminum module into full-automatic storage equipment through a manual sample inlet and outlet of the full-automatic storage equipment, wherein a freezing tube is placed in the pre-cooling aluminum module, and a sample is contained in the freezing tube;
step 2, the control system controls the liquid nitrogen tank to open a tank opening, then the mechanical arm A is controlled to take out the pre-cooling aluminum module through an automatic sample inlet and outlet of full-automatic storage equipment to a liquid nitrogen cooling assembly for cooling, a code reader reads a code of the pre-cooling aluminum module in the process of conveying the pre-cooling aluminum module, the type of the sample is identified, the type of the sample is transmitted to the control system through a signal, and the control system adjusts different cooling time and grinding time according to different types of the sample;
step 3, when the cooling time is up, the control system controls the mechanical arm A to clamp and place the pre-cooling aluminum module in the tissue grinder, and the sample in the cryopreservation tube in the pre-cooling aluminum module is ground;
and 4, after grinding is finished, controlling a mechanical arm A by the control system to clamp and place the pre-cooled aluminum module in full-automatic storage equipment, and taking the pipe frame provided with the ground cryopreserved pipe away from a manual sample inlet and outlet.
The preferred scheme is as follows:
preferably: the cooling time in the step 2 is 10 s-2 min. Different samples need different cooling time lengths, and the cooling time range is 10 s-2 min according to system calculation.
Preferably: the grinding frequency in the step 3 is 10-70 HZ/s, and the grinding time is 30 s-2 min. Different samples need different grinding frequencies and grinding durations, and the grinding frequency is 10-70 HZ/s and the time range is 30 s-2 min according to system calculation.
In the invention, the following preferable scheme can be added: the liquid nitrogen cooling assembly further comprises a liquid nitrogen supply tank, the liquid nitrogen tank is communicated with the liquid nitrogen supply tank through a pipeline, and the liquid nitrogen tank and the liquid nitrogen supply tank are both provided with liquid level sensors. And when the liquid level sensor displays that the liquid nitrogen in the liquid nitrogen tank is insufficient, the liquid nitrogen supply tank conveys the liquid nitrogen into the liquid nitrogen tank. The control system comprises a whole-course monitoring management module, a data storage module, a remote monitoring module and a recording report module. The whole-course monitoring management module can monitor the sample in the whole course for 24 hours through the monitoring equipment and send the monitored data to the whole-course monitoring management module, and the whole-course monitoring management module manages the operation of each component in the system through the monitored data and the pre-input instruction; the data storage module can automatically record and backup all monitored data, store the data and download the data as required, thereby meeting various authentication requirements; the remote monitoring module can inquire the monitoring state through any equipment which can be networked, and when the monitored value exceeds a preset value, early warning can be realized through remote control; the recording report module can form intelligent charts such as data reports, curve graphs and the like according to requirements, and is convenient for filing and big data analysis.
In conclusion, the invention has the following beneficial effects:
because be in the closed environment, guaranteed the accuracy nature after the sample grinds, take notes grinding data each time simultaneously, lightened staff's work burden, utilize machinery to cool off and grind in the safety ring border, ensured staff's safety again, through control system to full-automatic sample storage subassembly, arm A, carousel subassembly, code reader, liquid nitrogen cooling module and grinding component's control, accomplished full automatization, greatly improved grinding efficiency and quality.
Drawings
FIG. 1 is a flowchart of the operation of embodiment 1 of the present invention;
FIG. 2 is a flowchart of the operation of embodiment 2 of the present invention
Fig. 3 is a block schematic diagram of a control system.
In the figure, 1, a full-automatic storage device; 2. a pipe frame; 3. pre-cooling the aluminum module; 4. a mechanical arm A; 5. a mechanical arm B; 6. an operation table; 7. freezing and storing the tube; 8. a code reader; 9. a liquid nitrogen tank; 10. a tissue grinder; 11. a liquid nitrogen supply tank; 12. a liquid level sensor; 13. and (5) controlling the system.
Detailed Description
The present invention will be described in further detail with reference to the accompanying drawings.
In which like parts are designated by like reference numerals. It should be noted that the terms "front," "back," "left," "right," "upper" and "lower" used in the following description refer to directions in the drawings, and the terms "bottom" and "top," "inner" and "outer" refer to directions toward and away from, respectively, the geometric center of a particular component.
Example 1:
according to fig. 1, the system is located in a closed environment with a temperature ranging from 2 ℃ to 8 ℃, and comprises the following components:
the full-automatic sample storage assembly comprises a full-automatic storage device 1, a pre-cooling aluminum module 3 and a manual sample inlet and outlet, wherein the full-automatic storage device 1 is positioned on the front side of a closed environment and is provided with a manual sample inlet and outlet and an automatic sample inlet and outlet, the manual sample inlet and outlet is communicated with the outside of the closed environment, and the automatic sample inlet and outlet is communicated with the inside of the closed environment and is used for placing and conveying the pipe frame 2 and the pre-cooling aluminum module;
the mechanical arm A4 is positioned in the middle of the closed environment and used for clamping the pipe frame 2 and the precooling aluminum module 3;
the turntable assembly is positioned at the left side position of the closed environment and comprises a mechanical arm B5 and an operating platform 6, the mechanical arm B5 is positioned at the left side of the operating platform 6, and the mechanical arm B5 is used for clamping the freezing pipes 7 in the pipe racks 2 on the operating platform 6 into the pre-cooling aluminum modules 3;
the code reader 8 is positioned on a running line from the full-automatic sample storage component to the turntable component of the mechanical arm A4, is used for scanning codes on the pipe frame 2, identifying different sample types, and is used for placing the code reader 8 on the rear side of the left side part of the operating platform 6 for more convenient reading;
the liquid nitrogen cooling assembly is positioned at the rear side of the closed environment, comprises a liquid nitrogen tank 9 and a liquid nitrogen supply tank 11 and is used for cooling the pre-cooled aluminum module 3;
the grinding assembly, located on the right side of the enclosed environment, includes a tissue grinder 10 for grinding the sample in the cryopreservation tube 7 in the pre-cooled aluminum module 3.
And the control system is used for controlling the fully-automatic sample storage assembly, the mechanical arm A4, the turntable assembly, the code reader 8, the liquid nitrogen cooling assembly and the grinding assembly.
The control system is developed along with the authority management requirements of equipment operations such as cold chain monitoring (temperature and humidity), gas monitoring (oxygen concentration and carbon dioxide concentration), liquid level of the liquid nitrogen tank 9, the full-automatic storage equipment 1, sample taking and placing of the liquid nitrogen tank 9, the tissue grinder 10 and the like, and automatic real-time remote monitoring and control, remote alarm and data recording of a laboratory are realized. Respectively comprises the following steps: the whole-course monitoring management module can perform uninterrupted whole-course accurate monitoring management on a target object (the liquid nitrogen tank 9, the full-automatic storage device 1, the ambient temperature and the like) for 24 hours at any place through monitoring equipment, and sends monitored data to the whole-course monitoring management module, and the whole-course monitoring management module manages the operation of each component in the system through the monitored data and a pre-input instruction; the data storage module can automatically record (1 time in 10 minutes), backup all monitored data, permanently store the monitored data, download the data as required, realize data traceability and meet various authentication requirements; the remote monitoring module can inquire the monitoring state of the monitoring equipment through any equipment which can be networked, and when the monitored value exceeds a preset value, early warning can be realized through remote control; the recording report module can form intelligent charts such as data reports, curve graphs, bar charts and the like according to requirements, and is convenient for recording and archiving. The signal output part of the whole-course monitoring management module is connected with the signal input parts of the mechanical arm A4, the code reader 8, the mechanical arm B5, the liquid nitrogen tank 9 and the tissue grinder 10 respectively, the signal input part of the whole-course monitoring management module is connected with the signal output parts of the mechanical arm A4, the code reader 8, the mechanical arm B5, the liquid nitrogen tank 9 and the tissue grinder 10 respectively, and the whole-course monitoring management module is further connected with the following monitoring equipment respectively: the temperature and humidity wireless Sensor (such as Smart Sensor) has the capability of monitoring the temperature (-20-60 ℃) and the humidity (0-100% RH) of a laboratory, and transmits the acquired data to the whole-process monitoring management module in a wireless mode after acquiring the data of the temperature and the humidity; the liquid level temperature wireless sensor (such as Smart sensor LT) of the liquid nitrogen tank can monitor the liquid level (0-650 mm) of the liquid nitrogen tank 9 and the temperature (-200 ℃) of the inner opening of the tank, and when the liquid level and the temperature data in the liquid nitrogen tank 9 are collected, the collected data are transmitted to the whole-process monitoring management module in a wireless mode; the gas wireless Sensor (such as Smart Sensor) adopts an imported electrochemical Sensor (oxygen) or an infrared Sensor (carbon dioxide), has excellent sensitivity and repeatability, immediately alarms when the monitored data exceeds a preset alarm value, and simultaneously transmits the acquired data to the whole-process monitoring management module in a wireless mode; an intelligent union audible and visual alarm (such as Smartmonitor X) is a new generation high-end on-site audible and visual alarm with three network connection functions of mobile GPS/WIFI/wired network based on the Internet of things, cloud technology and android flat panel technology as cores, can realize on-site and remote alarm at the same time, ensures the storage safety of samples and performs data interaction with a control system in real time.
The specific procedure of example 1 was as follows:
step 1, manually placing the pre-cooling aluminum module 3 and the pre-cooling aluminum module 2 into full-automatic storage equipment 1 through a manual sample inlet and outlet respectively;
step 2, controlling a mechanical arm A4 by the control system, sequentially clamping and placing the pipe frame 2 and the pre-cooling aluminum module 3 in the full-automatic storage device 1 on the operation table 6 through an automatic sample inlet and outlet port on the full-automatic storage device 1, placing the pipe frame 2 on the rear side of the upper part of the table top of the operation table 6, and placing the pre-cooling aluminum module 3 on the front side of the upper part of the table top of the operation table 6;
and 3, when the control system receives the electric signals of the mechanical arm A4 conveying pipe frame 2 and the precooling aluminum module 3, the control system controls the code reader 8 to start reading codes.
And 4, when the control system receives an electric signal that the code reader 8 sends a code reading completion, the control system sends an instruction to a mechanical arm B5 to a mechanical arm B5, the freezing tubes 7 on the tube frame 2 are clamped one by one and placed into the pre-cooling aluminum module 3, when all the freezing tubes 7 are placed into the pre-cooling aluminum module 3, the control system controls the liquid nitrogen tank 9 to open the tank opening, then the control system controls the mechanical arm A4 to clamp the pre-cooling aluminum module 3 and place the pre-cooling aluminum module 9 into the liquid nitrogen tank 9, and the control system adjusts the time required to be cooled to 10 s-2 min according to the previous code reading.
Step 5, after cooling, controlling a mechanical arm A4 to clamp and place the pre-cooled aluminum module 3 in a tissue grinder 10 by a control system, then withdrawing the mechanical arm A4 from the tissue grinder 10, fixing the sample in a cryopreservation tube 7 in the pre-cooled aluminum module 3 by the tissue grinder 10, and adjusting the grinding frequency to be 10-70 HZ/s and the grinding time to be 30 s-2 min by the control system according to the code reading;
step 6, the control system receives electric signals sent by various tissue grinding instruments 10 for finishing grinding, controls the mechanical arm A4 to clamp and take out the pre-cooled aluminum module 3 from the tissue grinding instrument 10 and place the pre-cooled aluminum module on the operating platform 6, and then controls the mechanical arm B5 to clamp and place the freezing tube 7 in the pre-cooled aluminum module 3 into the tube frame 2;
and 7, receiving an electric signal of clamping completion by the mechanical arm B5 by the control system, controlling the mechanical arm A4 to respectively clamp the pipe frame 2 and the precooling aluminum module 3 to be placed in the full-automatic storage equipment 1 through an automatic sample inlet and outlet, and manually taking the pipe frame 2 provided with the grinded cryopreservation pipe 7 from the sample inlet and outlet.
In operation, since the cooling tube can be directly placed in the pre-cooling aluminum module for subsequent cooling grinding, and the turntable assembly is not required to perform turntable post-cooling grinding on the cooling tube, example 2 is as follows:
according to fig. 2, the system is located in a closed environment with a temperature ranging from 2 ℃ to 8 ℃, and comprises the following components:
the full-automatic sample storage assembly comprises a full-automatic storage device 1, a sample inlet and outlet device and a pre-cooling aluminum module 3, wherein the full-automatic storage device 1 is positioned on the front side of a closed environment and is provided with a manual sample inlet and outlet port and an automatic sample inlet and outlet port, the manual sample inlet and outlet port is communicated with the outside of the closed environment, and the automatic sample inlet and outlet port is communicated with the inside of the closed environment and is used for placing and conveying the pre-cooling aluminum module;
the mechanical arm A4 is positioned in the middle of the closed environment and used for clamping the pre-cooled aluminum module 3;
the code reader 8 is positioned on the full-automatic storage equipment 1 and used for scanning codes on the pipe frame 2 and identifying different sample types;
the liquid nitrogen cooling assembly is positioned at the rear side of the closed environment, comprises a liquid nitrogen tank 9 and a liquid nitrogen supply tank 11 and is used for cooling the pre-cooled aluminum module 3;
the grinding assembly, located on the right side of the enclosed environment, includes a tissue grinder 10 for grinding the sample in the cryopreservation tube 7 in the pre-cooled aluminum module 3.
And the control system is used for controlling the fully-automatic sample storage assembly, the mechanical arm A4, the code reader 8, the liquid nitrogen cooling assembly and the grinding assembly.
The control system is developed along with the authority management requirements of equipment operations such as cold chain monitoring (temperature and humidity), gas monitoring (oxygen concentration and carbon dioxide concentration), liquid level of the liquid nitrogen tank 9, the full-automatic storage equipment 1, sample taking and placing of the liquid nitrogen tank 9, the tissue grinder 10 and the like, and automatic real-time remote monitoring and control, remote alarm and data recording of a laboratory are realized. Respectively comprises the following steps: the whole-course monitoring management module can perform uninterrupted whole-course accurate monitoring management on a target object (the liquid nitrogen tank 9, the full-automatic storage device 1, the ambient temperature and the like) for 24 hours at any place through monitoring equipment, and sends monitored data to the whole-course monitoring management module, and the whole-course monitoring management module manages the operation of each component in the system through the monitored data and a pre-input instruction; the data storage module can automatically record (1 time in 10 minutes), backup all monitored data, permanently store the monitored data, download the data as required, realize data traceability and meet various authentication requirements; the remote monitoring module can inquire the monitoring state of the monitoring equipment through any equipment which can be networked, and when the monitored value exceeds a preset value, early warning can be realized through remote control; the recording report module can form intelligent charts such as data reports, curve graphs, bar charts and the like according to requirements, and is convenient for recording and archiving. The signal output part of the whole-course monitoring management module is connected with the signal input parts of the mechanical arm A4, the code reader 8, the mechanical arm B5, the liquid nitrogen tank 9 and the tissue grinder 10 respectively, the signal input part of the whole-course monitoring management module is connected with the signal output parts of the mechanical arm A4, the code reader 8, the mechanical arm B5, the liquid nitrogen tank 9 and the tissue grinder 10 respectively, and the whole-course monitoring management module is further connected with the following monitoring equipment respectively: the temperature and humidity wireless Sensor (such as Smart Sensor) has the capability of monitoring the temperature (-20-60 ℃) and the humidity (0-100% RH) of a laboratory, and transmits the acquired data to the whole-process monitoring management module in a wireless mode after acquiring the data of the temperature and the humidity; the liquid level temperature wireless sensor (such as Smart sensor LT) of the liquid nitrogen tank can monitor the liquid level (0-650 mm) of the liquid nitrogen tank 9 and the temperature (-200 ℃) of the inner opening of the tank, and when the liquid level and the temperature data in the liquid nitrogen tank 9 are collected, the collected data are transmitted to the whole-process monitoring management module in a wireless mode; the gas wireless Sensor (such as Smart Sensor) adopts an imported electrochemical Sensor (oxygen) or an infrared Sensor (carbon dioxide), has excellent sensitivity and repeatability, immediately alarms when the monitored data exceeds a preset alarm value, and simultaneously transmits the acquired data to the whole-process monitoring management module in a wireless mode; an intelligent union audible and visual alarm (such as Smartmonitor X) is a new generation high-end on-site audible and visual alarm with three network connection functions of mobile GPS/WIFI/wired network based on the Internet of things, cloud technology and android flat panel technology as cores, can realize on-site and remote alarm at the same time, ensures the storage safety of samples and performs data interaction with a control system in real time.
The specific procedure of example 2 was as follows:
step 1, manually placing a pre-cooling aluminum module 3 provided with a freezing tube 7 into full-automatic storage equipment 1 through a manual sample inlet and outlet respectively;
step 2, the control system controls the liquid nitrogen tank 9 to open a tank opening, simultaneously controls the code reader 8 on the full-automatic storage device 1 to start code reading, and then controls the mechanical arm A4 to clamp the pre-cooled aluminum module 3 and place the pre-cooled aluminum module into the liquid nitrogen tank 9;
and 3, the control system receives an electric signal which is sent by the code reader 8 and finishes code reading, and adjusts the time needing cooling to be 10 s-2 min according to the previous code reading.
Step 4, after cooling, controlling a mechanical arm A4 to clamp and place the pre-cooled aluminum module 3 in a tissue grinder 10 by a control system, then withdrawing the mechanical arm A4 from the tissue grinder 10, fixing the sample in a cryopreservation tube 7 in the pre-cooled aluminum module 3 by the tissue grinder 10, and adjusting the grinding frequency to be 10-70 HZ/s and the grinding time to be 30 s-2 min by the control system according to the code reading;
and step 5, the control system receives an electric signal which is sent by the multiple tissue grinding 10 instrument and is used for finishing grinding, the mechanical arm A44 precooling aluminum module 3 is controlled by the 4 control mechanical arm A to be placed in the full-automatic storage device 1 through the automatic sample inlet and outlet, and the pipe frame 2 provided with the frozen tube 7 after grinding treatment is taken away from the manual sample inlet and outlet.
The present embodiment is only for explaining the present invention, and it is not limited to the present invention, and those skilled in the art can make modifications of the present embodiment without inventive contribution as needed after reading the present specification, but all of them are protected by patent law within the scope of the claims of the present invention.

Claims (10)

1. A sample polishing system in a closed environment, comprising:
the full-automatic sample storage assembly comprises a full-automatic storage device (1), wherein the full-automatic storage device (1) is provided with a manual sample inlet and outlet and an automatic sample inlet and outlet, the manual sample inlet and outlet is communicated with the outside of a closed environment, the automatic sample inlet and outlet is communicated with the inside of the closed environment, and the full-automatic storage device (1) is used for placing and conveying a pipe frame (2) and a pre-cooling aluminum module (3);
the mechanical arm A (4) is used for clamping the pipe frame (2) and the precooling aluminum module (3);
the rotary table assembly comprises a mechanical arm B (5) and an operating platform (6), wherein the mechanical arm B (5) is used for clamping a freezing pipe (7) in the pipe frame (2) on the operating platform (6) into the pre-cooling aluminum module (3);
the code reader (8) is positioned on a running line from the full-automatic sample storage component to the turntable component of the mechanical arm A (4) and is used for scanning codes on the pipe frame (2) and identifying different sample types;
the liquid nitrogen cooling assembly comprises a liquid nitrogen tank (9) for cooling the pre-cooling aluminum module (3);
a grinding assembly comprising a tissue grinder (10) for grinding the sample in the cryopreservation tube (7) in the pre-cooled aluminium module (3);
and the control system is used for controlling the full-automatic sample storage assembly, the mechanical arm A (4), the turntable assembly, the code reader (8), the liquid nitrogen cooling assembly and the grinding assembly.
2. A sample polishing system in a closed environment, comprising:
the full-automatic sample storage assembly comprises a full-automatic storage device (1), wherein the full-automatic storage device (1) is provided with a manual sample inlet and outlet and an automatic sample inlet and outlet, the manual sample inlet and outlet is communicated with the outside of a closed environment, the automatic sample inlet and outlet is communicated with the inside of the closed environment, and the full-automatic storage device (1) is used for placing and conveying a pre-cooling aluminum module (3);
the mechanical arm A (4) is used for clamping the pre-cooled aluminum module (3);
the liquid nitrogen cooling assembly comprises a liquid nitrogen tank (9) for cooling the pre-cooling aluminum module (3);
the code reader (8) is positioned on a running line from the full-automatic sample storage component to the liquid nitrogen cooling component of the mechanical arm A (4) and is used for scanning codes of the pre-cooling aluminum module (3) and identifying different sample types;
a grinding assembly comprising a tissue grinder (10) for grinding the sample in the cryopreservation tube (7) in the pre-cooled aluminium module (3);
and the control system is used for controlling the full-automatic sample storage assembly, the mechanical arm A (4), the code reader (8), the liquid nitrogen cooling assembly and the grinding assembly.
3. The sample grinding work system according to claim 1 or 2, wherein the liquid nitrogen cooling assembly further comprises a liquid nitrogen supply tank (11), the liquid nitrogen tank (9) is communicated with the liquid nitrogen supply tank (11) through a pipeline, and the liquid nitrogen tank (9) and the liquid nitrogen supply tank (11) are both provided with liquid level sensors (12).
4. A sample grinding work system according to claim 1 or 2, wherein the control system comprises a global monitoring management module, a data storage module, a remote monitoring module and a record reporting module.
5. The working method of the sample grinding working system as claimed in claim 1, characterized by comprising the following specific operation steps:
step 1, respectively placing a pipe frame (2) and a pre-cooling aluminum module (3) into full-automatic storage equipment (1) through a manual sample inlet and outlet of the full-automatic storage equipment, wherein a freezing tube (7) is placed in the pipe frame (2), and a sample is contained in the freezing tube (7);
step 2, the control system controls a mechanical arm A (4) to sequentially take out the pipe frame (2) and the pre-cooling aluminum module (3) through an automatic sample inlet and outlet port of the full-automatic storage device (1) and place the pipe frame and the pre-cooling aluminum module on an operation table (6), a code reader (8) reads codes of the pipe frame (2) in the process of conveying the pipe frame (2), the sample type is identified, the sample type is transmitted to the control system through signals, and the control system adjusts different cooling time and grinding time according to different sample types;
step 3, controlling a mechanical arm B (4) to clamp and place a freezing tube (7) in a tube frame (2) on an operation table (6) into the pre-cooling aluminum module (3);
step 4, controlling a liquid nitrogen tank (9) to open a tank opening by a control system, and then controlling a mechanical arm A (4) to clamp the pre-cooled aluminum module (3) filled with the freezing storage pipe (7) and placing the pre-cooled aluminum module into the liquid nitrogen tank (9) for cooling;
step 5, when the cooling time is up, the control system controls the mechanical arm A (4) to clamp the pre-cooling aluminum module (3) and place the pre-cooling aluminum module in the tissue grinder (10), and a sample in the cryopreservation tube (7) in the pre-cooling aluminum module (3) is ground;
step 6, after grinding is finished, the control system controls the mechanical arm A (4) to clamp and place the pre-cooled aluminum module (3) on the operating table (6);
step 7, controlling a mechanical arm B (4) to clamp and place the cryopreserved pipe (7) in the pre-cooling aluminum module (3) into the pipe frame (2) by the control system;
and 8, controlling a mechanical arm A (4) to respectively clamp the pipe frame (2) and the pre-cooling aluminum module (3) to be placed in the full-automatic storage equipment (1) through an automatic sample inlet and outlet, and taking the pipe frame (2) provided with the frozen pipe (7) after grinding treatment away from the manual sample inlet and outlet.
6. The working method of the sample grinding working system as claimed in claim 5, wherein the cooling time in step 4 is 10 s-2 min.
7. The working method of the sample grinding working system as claimed in claim 5, wherein the grinding frequency in step 5 is 10 to 70 Hz/s, and the grinding time is 30s to 2 min.
8. The working method of the sample grinding working system as claimed in claim 2, characterized by comprising the following specific operation steps:
step 1, respectively placing a pre-cooling aluminum module (3) into full-automatic storage equipment (1) through a manual sample inlet and outlet of the full-automatic storage equipment, wherein a freezing tube (7) is placed in the pre-cooling aluminum module (3), and the freezing tube (7) contains a sample;
step 2, the control system controls a liquid nitrogen tank (9) to open a tank opening, then controls a mechanical arm A (4) to take out the pre-cooling aluminum module (3) through an automatic sample inlet and outlet port of the full-automatic storage device (1) to a liquid nitrogen cooling assembly for cooling, in the process of conveying the pre-cooling aluminum module (3), a code reader (8) reads codes of the pre-cooling aluminum module (3), identifies sample types, transmits the sample types to the control system through signals, and the control system adjusts different cooling time and grinding time according to different sample types;
step 3, when the cooling time is up, the control system controls the mechanical arm A (4) to clamp the pre-cooling aluminum module (3) and place the pre-cooling aluminum module in the tissue grinder (10), and a sample in the cryopreservation tube (7) in the pre-cooling aluminum module (3) is ground;
and 4, after grinding is finished, controlling a mechanical arm A (4) to clamp the pre-cooled aluminum module (3) and place the pre-cooled aluminum module in the full-automatic storage device (1), and taking the pipe frame (2) provided with the ground cryopreserved pipe (7) away from a manual sample inlet and outlet.
9. The working method of the sample grinding working system as claimed in claim 8, wherein the cooling time in the step 2 is 10 s-2 min.
10. The working method of the sample grinding working system as claimed in claim 8, wherein the grinding frequency in step 3 is 10 to 70 HZ/sec, and the grinding time is 30s to 2 min.
CN201911266311.1A 2019-12-11 2019-12-11 Working system and working method for sample grinding Withdrawn CN110791424A (en)

Priority Applications (1)

Application Number Priority Date Filing Date Title
CN201911266311.1A CN110791424A (en) 2019-12-11 2019-12-11 Working system and working method for sample grinding

Applications Claiming Priority (1)

Application Number Priority Date Filing Date Title
CN201911266311.1A CN110791424A (en) 2019-12-11 2019-12-11 Working system and working method for sample grinding

Publications (1)

Publication Number Publication Date
CN110791424A true CN110791424A (en) 2020-02-14

Family

ID=69447982

Family Applications (1)

Application Number Title Priority Date Filing Date
CN201911266311.1A Withdrawn CN110791424A (en) 2019-12-11 2019-12-11 Working system and working method for sample grinding

Country Status (1)

Country Link
CN (1) CN110791424A (en)

Cited By (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN117388031A (en) * 2023-12-08 2024-01-12 新乡市食品药品检验所 Pretreatment manipulator for sampling detection sample

Cited By (2)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN117388031A (en) * 2023-12-08 2024-01-12 新乡市食品药品检验所 Pretreatment manipulator for sampling detection sample
CN117388031B (en) * 2023-12-08 2024-02-23 新乡市食品药品检验所 Pretreatment manipulator for sampling detection sample

Similar Documents

Publication Publication Date Title
CN107686811B (en) Stem cell manufacturing system, information management system, transportation and cryopreservation device
US11540248B2 (en) Mobile monitoring device for controlled contamination areas
US9851299B2 (en) Method of analyzing air quality
CN211284388U (en) Sample grinding work system
CN110791424A (en) Working system and working method for sample grinding
MXPA02001451A (en) Automated sample handling for x-ray crystallography.
WO2015076391A1 (en) Automatic culture system and cell management system
WO2022247845A1 (en) Cell program freezing method, system and apparatus
CN106244456A (en) Fully-automatic intelligent incubator and control method thereof
CN105505762A (en) Dynamic monitoring type bacterial culture equipment
CN109387536A (en) A kind of automation dry and wet roadbed freezing and thawing circulating test device and test method
CN206348017U (en) A kind of pipeline system vegetation monitoring system
EP3020276A1 (en) Method and apparatus for collecting and preserving biological specimens
US20160066565A1 (en) Isolator devices for collection, parametrical characterization and long-term preservation of organic fluids and/or materials containing stem cells
RU134327U1 (en) DEVICE FOR DETERMINING HARMFUL SUBSTANCES IN THE AIR
US20140377843A1 (en) Automated microbiology laboratory instrument and system uses thereof
CN203443837U (en) Visual cross-section grinding device
CN105532638A (en) Instrument apparatus for low-temperature storage of biological material
CN110358684A (en) A kind of automatic culture apparatus of cell and its application method
EP3500862B1 (en) Automated transport unit and system
CN115895880A (en) Embryo freezing thawing machine
JP4233372B2 (en) Ultra-low temperature calibration method and ultra-low temperature calibration device
CN209311368U (en) A kind of automation dry and wet roadbed freezing and thawing circulating test device
CN104743532B (en) Surrounding air Kr-85 concentration device under room temperature
WO2021028942A1 (en) A soil analysis apparatus

Legal Events

Date Code Title Description
PB01 Publication
PB01 Publication
SE01 Entry into force of request for substantive examination
SE01 Entry into force of request for substantive examination
WW01 Invention patent application withdrawn after publication

Application publication date: 20200214

WW01 Invention patent application withdrawn after publication