CN110791411A

CN110791411A - Multi-scene and multi-strain incubator

Info

Publication number: CN110791411A
Application number: CN201911197357.2A
Authority: CN
Inventors: 顾兵; 杨英; 胡珊; 顾锋; 毛佩; 康海全
Original assignee: Xuzhou Medical University
Current assignee: Xuzhou Medical University
Priority date: 2019-11-29
Filing date: 2019-11-29
Publication date: 2020-02-14

Abstract

The invention discloses a multi-scene and multi-strain incubator, belonging to the technical field of microbial cultivation equipment. The incubator comprises a three-in-one incubator body, a single chip microcomputer, a control module, a display module, a power circuit, 3 temperature and humidity sensors and 1 CO arranged in the incubator body₂Sensor, heater, humidifier and CO₂And supplying the equipment. The multi-scene and multi-strain incubator provided by the invention has the advantages of scientific and reasonable design, simplicity and convenience in operation, and capability of adjusting the temperature and humidity in the incubator bodyDegree and CO₂The concentration is accurately and stably automatically controlled and adjusted, various conditions of different bacteria and fungi in the growth environments of different boxes are maintained in the most appropriate range, the simultaneous culture of different strains and the culture under different environments can be realized, the success rate of the bacteria and fungi culture is high, and the requirements of practical application can be well met.

Description

Multi-scene and multi-strain incubator

Technical Field

The invention belongs to the technical field of microorganism culture equipment, and particularly relates to a multi-scene and multi-strain incubator.

Background

With the development of the information age, the popularization of intelligent products has been related to various fields of society, and the intelligence of medical equipment has become a necessary trend. The culture method mainly utilizes an artificial method to breed, namely, strains are inoculated on a culture medium to grow and breed, and the whole culture process must be finished according to aseptic operation steps, otherwise, result errors or cross infection can be caused. In addition, the culture method and culture conditions of each strain are different, and a special culture medium and strict culture temperature, humidity and CO are required₂Concentration, etc. The microorganism culture equipment of prior art can not provide above-mentioned harsh cultivation environment well on the one hand, leads to cultivateing failure rate higher, and on the other hand cultivates the environment comparatively singly, can not satisfy the user demand. The incubator in the current market can only cultivate single strains in a single environment, can not cultivate the same or different strains in a single or different environment, and has lower cultivation efficiency and smaller application range.

Disclosure of Invention

In view of the problems in the prior art, the present invention is to provide a multi-scenario, multi-strain incubator which can avoid the above technical defects.

In order to achieve the above object, the present invention provides the following technical solutions:

the utility model provides an incubator of many scenes, many bacterial, its characterized in that, including three-in-one incubator body of aerobic bacteria, anaerobe and fungi, singlechip, control module, display module, power supply circuit and 3 humiture sensing of setting in the box1 CO in the vessel₂Sensor, heater, humidifier and CO₂A supply device;

the control module, the display module, the temperature and humidity sensor and the CO₂The sensor, the heater and the humidifier are all connected with the single chip microcomputer;

the control module comprises a plurality of keys and a connecting circuit for connecting the keys with the singlechip and is used for sending instructions to the singlechip to set a temperature value, a humidity value and CO in the box body₂Concentration;

the temperature and humidity sensor is used for measuring temperature values and humidity values in three different boxes in real time and transmitting the measured values to the single chip microcomputer;

the CO is₂The sensor is used for measuring CO in the incubator body in real time₂The concentration value is transmitted to the single chip microcomputer;

after the single chip microcomputer receives the information, the temperature, the humidity and the CO are measured₂The control circuit regulates and controls the digital value to be displayed on the LED nixie tube display screen in real time;

the display module is used for displaying temperature, humidity and CO in real time₂The concentration value.

Further, the heater is a heating wire heater.

Furthermore, the display module is an LED nixie tube display screen.

Furthermore, the display module is an LED nixie tube display screen and comprises 3 double 8-shaped nixie tubes.

Further, the temperature and humidity sensor is STH 11.

Further, the CO is₂The sensor is MH-410D.

Further, the singlechip is an STC89C52 singlechip.

Furthermore, the multi-scene and multi-strain incubator can be used for adjusting the temperature, the humidity and the CO in the incubator body₂The concentration is dynamically obtained and adjusted in real time;

the real-time acquisition comprises the following steps:

step 1) calculating control parameters A, B, C; A. b and C represent temperature, humidity and carbon dioxide concentration, respectively;

step 2), setting an initial value e (n-1) ═ e (n-2) ═ 0;

step 3) inputting y (n) for the current sampling;

step 4), calculating e (n) and △ u (n);

step 5) outputting u (n) ═ u (n-1) + △ u (n), resetting e (n-2) ═ e (n-1), e (n-1) ═ e (n);

and 6) when the sampling time is reached, turning to the step 3) to obtain the next culture environment parameters.

The adjustment comprises the following steps:

according to the obtained information data, the single chip microcomputer queries a corresponding fuzzy control table according to the system error and the deviation change rate to obtain K_P，K_I，K_dPID operation is carried out on the setting values of the three parameters;

the incremental PID is only related to the nearest n-2, n-1, n times of errors, and the single chip microcomputer only needs to store the errors for three times;

the incremental PID formula is: u (n) ═ K_p(1+T/T_i+T_d/T)e_n-K_p(1+2T_d/T)e_n-1+K_p(T_d/T)e_n-2+ u (n-1), wherein u (n), u (n-1) are output values at the parameter acquisition time n, n-1 respectively; k_p，T_i，T_dProportional, integral and differential coefficients, respectively; e.g. of the type_n，e_n-1，e_n-2Obtaining deviation values at the time n, n-1 and n-2 for the parameters respectively; t is a sampling period;

A＝K_p(1+T/T_i+T_d/T)；B＝K_p(1+2T_d/T)；C＝K_p(T_dt); A. b and C represent temperature, humidity and carbon dioxide concentration, respectively.

Further, the inside thermal-insulated cladding that all uses thermal-insulated cotton and thermal-insulated silica gel of trinity cultivation box covers, thermal-insulated silica gel is used with the one side of box contact to thermal-insulated cladding, and thermal-insulated cotton is connected with the box through thermal-insulated silica gel.

Compared with the prior art, the invention has the following beneficial effects: the invention provides a multi-scene and multi-strain culture methodThe cultivation box has scientific and reasonable design and simple and convenient operation, and can carry out temperature, humidity and CO treatment on aerobic bacteria, anaerobic bacteria and fungi in three different boxes₂The concentration is respectively and accurately and stably controlled and adjusted, the growth environment conditions of aerobic bacteria, anaerobic bacteria and fungi are maintained in the most appropriate range, the culture success rate is high, the simultaneous culture of different strains in different environments can be realized, and the requirements of practical application can be well met.

Drawings

FIG. 1 is a block diagram of the circuit configuration of the present invention;

FIG. 2 is a timing diagram of the start transmission of the temperature and humidity sensor STH 11;

FIG. 3 is a timing diagram illustrating the communication reset of the temperature and humidity sensor STH 11;

FIG. 4 is a pin wiring diagram of temperature and humidity sensor STH 11;

FIG. 5 is a circuit diagram of the humidifier and heater connected together;

FIG. 6 is a pin wiring diagram of a control circuit for the heater;

FIG. 7 is a pin wiring diagram of a control circuit of the humidifier;

FIG. 8 shows CO₂A schematic structural diagram of the supply device;

FIG. 9 shows CO₂A concentration sampling and control circuit diagram;

FIG. 10 is a diagram of a reset circuit and a start-up circuit;

FIG. 11 is a key circuit diagram;

FIG. 12 is a circuit diagram of a nixie tube display;

FIG. 13 is a power supply circuit diagram;

FIG. 14 is a flow chart of a sampling process;

FIG. 15 is a schematic diagram of PID operation;

in the figure, 1-three-in-one incubator; 2-mixed gas cylinder; 3-a pressure gauge; 4-three-way valves; 5-a buffer bottle; 6-vacuum pump.

Detailed Description

In order to make the objects, technical solutions and advantages of the present invention more apparent, the following description is further made with reference to the accompanying drawings and specific embodiments. It should be understood that the specific embodiments described herein are merely illustrative of the invention and are not intended to limit the invention. All other embodiments, which can be derived by a person skilled in the art from the embodiments given herein without making any creative effort, shall fall within the protection scope of the present invention.

Example 1

As shown in figures 1-13, a multi-scene and multi-strain incubator comprises a three-in-one incubator body for aerobic bacteria, anaerobic bacteria and fungi, a single chip microcomputer, a control module, a display module, a power supply circuit, 3 temperature and humidity sensors, 1 CO, and a temperature and humidity sensor, 1 CO₂Sensor, heater, humidifier and CO₂A supply device; control module, display module, temperature and humidity sensor and CO₂The sensor, the heater and the humidifier are all connected with the single chip microcomputer. The singlechip is STC89C52 singlechip, and the temperature and humidity sensor is STH11, CO₂The sensor is MH-410D, the heater is an electric heating wire heater, the control module comprises a plurality of keys and a connecting circuit for connecting the keys with the STC89C52 single chip microcomputer, and the display module is an LED nixie tube display screen.

And the keys of the control module are adopted for input control, and different environmental parameters of different boxes are respectively set. Temperature and humidity values, CO, in three different boxes are measured in real time through a temperature and humidity sensor STH11₂Sensor MH-410D measures CO in anaerobic bacteria incubator in real time₂Concentration value, and sending the measured values to STC89C52 single chip microcomputer, processing by STC89C52 single chip microcomputer, and processing temperature, humidity and CO₂The control circuit regulates and controls, and the numerical values are respectively displayed on the LED nixie tube display screen in real time. The LED nixie tube is suitable for displaying numbers and low in cost, and occupies few singlechip port lines when being connected with the singlechip by adopting a dynamic scanning method, and programming of related display driving programs is relatively easy. The culture environment parameter display module reads data and respectively displays the current temperature, humidity and CO in different boxes through 3 double 8-digit code tubes₂And (4) concentration.

A plurality of keys of the control module send instructions to the STC89C52 singlechip to set the temperature value, the humidity value and the CO in the box body₂The concentration ranges are 0-50 ℃, 0-95% and 0-5%, and the error of each value is kept at +/-0.5 ℃ and +/-0.5%. Since the incubator requires fewer keys, a free standing key interface design is chosen. The independent keys are single key circuits directly formed by I/O port lines, and are characterized in that each key is independently provided with one I/O port line, and the working of each key does not influence the states of other I/O port lines. The key detection module is a part for realizing the temperature setting of a user, and the set temperature is between 0 and 50 ℃. Wherein S1 is a temperature-raising key, S2 is a temperature-lowering key, S3 is a confirming key, and S4 and S5 have no key values.

The temperature and humidity sensor STH11 is a temperature and humidity composite sensor with calibrated digital signal output. Micromachining proprietary technology using cmos process

The product has extremely high reliability and excellent long-term stability, comprises a capacitance type polymer humidity measuring element and an energy gap type temperature measuring element, is seamlessly connected with a 14-bit A/D converter and a serial interface circuit on the same chip, and has the advantages of excellent quality, ultrafast response, strong anti-interference capability, high cost performance and the like. The SHT11 uses a two-wire bi-directional serial interface that is optimized for both sensor signal reading and power consumption.

(1) The serial clock input SCK is used for communication synchronization between the microprocessor and the SHT 11. Since the interface contains completely static logic, there is no minimum SCK frequency.

(2) The serial DATA tri-state gate is used for reading DATA. DATA changes state after the SCK clock falling edge and is only valid on the SCK clock rising edge. During DATA transmission, DATA must remain stable at SCK clock high. To avoid signal collision, the microprocessor should drive DATA low.

The method for detecting the temperature and the humidity by using the temperature and humidity sensor STH11 comprises the following steps:

starting a sensor: firstly, the sensor is electrified after the work order voltage is selected, and the electrifying rate is not lower than 1V/ms. The sensor takes 11ms to go to sleep on a telephone without allowing any commands to be sent to the sensor before.

Sending a command: the initialization of the data transfer is represented by a set of "start transfer" sequences. It includes: DATA toggles low when the SCK clock is high, followed by SCK going low, followed by DATA toggling high at the SCK clock high.

The subsequent command contains three address bits (currently only "000" is supported), and five command bits. The SHT11 will indicate that the instruction was received correctly in the following manner: after the 8 th falling edge of the SCK clock, DATA is pulled down to low (ACK bit). After the falling edge of the 9 th SCK clock, DATA is released (high level is restored). The STH11 command set is shown in table 1.

TABLE 1 STH11 Command set

Measurement timing (RH and T): after issuing a set of measurement commands ('00000101' for relative humidity RH, '00000011' for temperature T), the controller waits for the end of the measurement. This process takes about 20/80/320ms, corresponding to 8/12/14bit measurements respectively. The exact time may vary by-30% at most depending on the internal crystal speed. The SHT11 indicates the end of the measurement by pulling DATA low and entering idle mode. The controller must wait for this "data ready" signal to read the data before triggering the SCK clock again. The sensed data may be stored first so that the controller can continue to perform other tasks and read the data again when needed.

Then 2 bytes of measurement data and 1 byte of CRC parity are transmitted. uC needs to acknowledge each byte by pulling DATA low. All data starts with the MSB and the right value is valid (e.g., for 12-bit data, the MSB is counted from the 5 th SCK clock; for 8-bit data, the first byte is meaningless).

The end of the communication is indicated by the acknowledge bit of the CRC data. If no CRC-8 check is used, the controller may abort the communication by holding the acknowledge bit ack high after measuring the LSB.

After the measurement and communication are finished, the SHT11 automatically goes into sleep mode.

To ensure that the self temperature rise is below 0.1 ℃, the activation time of SHT11 should not exceed 10% (e.g., up to 2 measurements per second for a 12bit accuracy measurement).

Communication reset time sequence: if communication with the SHT11 is interrupted, the serial port may be reset by the following signal sequence: when DATA remains high, the SCK clock is triggered 9 or more times. Before the next command, a "transmission start" sequence is sent. These timings only reset the serial port and the status register contents remain.

CRC-8 checking: the whole transmission process of the digital signal is ensured by 8-bit check. Any erroneous data will be detected and cleared.

The output is converted into a physical quantity: to compensate for the non-linearity of the humidity sensor to obtain accurate data, the formula RH is used_linear＝c₁+c₂·SO_RH+c₃·SO_RH ²And correcting the output value. The humidity conversion coefficient is shown in table 2.

TABLE 2 humidity conversion factor

Temperature compensation of the relative humidity of the humidity sensor: when the actual measured temperature differs significantly from 25 ℃ (77 ° f), the temperature correction factor of the humidity sensor should be considered: RH (relative humidity)_true＝(T_℃-25)·(t₁+t₂·SO_RH)+RH_linearThe temperature compensation coefficients are shown in table 3.

TABLE 3 temperature Compensation coefficients

Temperature: byThe temperature sensor developed by the bandgap material PTAT (proportional to absolute temperature) has excellent linearity. The formula Temperature can be used as d₁+d₂·SO_TThe digital output is converted to a temperature value. The temperature conversion coefficient is shown in table 4.

TABLE 4 temperature conversion coefficient

A control circuit of the heater and the humidifier adopts a silicon type optocoupler MOC3041M with bidirectional controllable zero crossing, integrates the functions of zero crossing triggering, zero crossing detection, photoelectric isolation and the like, and avoids the defect that an input channel and an output channel simultaneously control the triggering of a bidirectional thyristor. The interface of the STC89C52 singlechip is utilized, the output current is amplified through the triode, the direct current control end of the SSR is directly operated, and the purpose of controlling the heater and the humidifier is achieved.

The humidifier is an ultrasonic humidifier. When the ultrasonic humidifier works, the control valve purifies water in the water tank through the water purifier and then injects the purified water into the atomization pool. The energy converter converts high-frequency electric energy into mechanical vibration, water in the atomization pool is treated into ultra-fine particle mist, and the mist is blown into the box under the action of air flow generated by a fan, so that the task of humidifying air is completed.

A power supply circuit: after the single chip microcomputer is connected with a 220V power supply through a humidifier control circuit, the potentiometer W1 is rotated to enable the contact of the potentiometer W1 to be connected, and after 220V mains supply voltage is input through the fuse FU, the first path supplies power to the heater circuit through the bidirectional thyristor; the second path is stepped down by a transformer T to output two kinds of alternating voltages of 72V and 12V. The 72V alternating voltage is rectified by the bridge rectifier, and C1 generates about 72V direct voltage after filtering, so that the converter D and the oscillating tube Q6 are powered, and the indicator lamp D1 emits light by limiting current through R12, which indicates that the power supply circuit works; the 12V alternating current voltage is rectified by the bridge rectifier stack and then filtered by the C7 to supply power for the direct current fan motor.

And (3) spray control: when the switch contact of the potentiometer W1 is turned on and the water level in the container is normal, the voltage at the two ends of the C1 passes through the S3, the R7 leads the Q5 to be conducted, the voltage output by the e pole of the Q5 is added to the b pole of the oscillating tube Q6 through the R10 and the R11, the Q6 is vibrated in an inductance three-point oscillator consisting of the L1, the L2, the C3 and the like, the generated pulse voltage leads the transducer D to generate high-frequency vibration, and finally, the water in the water box is atomized and blown into the room under the coordination of the fan motor, so that the aim of humidification is fulfilled.

The adjustment potentiometer W1 can change the b pole current of the oscillation tube Q6, so that the amplification factor of the input signal of the oscillator can be changed, the oscillation amplitude of the transducer D is controlled, and the humidification strength is controlled.

W2 is an adjustable resistor for setting the maximum mist amount and the power of the whole machine.

A heating circuit: when hot mist humidification is needed, the single chip microcomputer is connected with a hot mist/cold mist switch S4 through a heater control circuit, voltage at two ends of a C1 supplies power to a light emitting tube in the photoelectric coupler N through R13, the light emitting tube starts to emit light, and a photosensitive tube in the N is conducted after being illuminated. After the photosensitive tube is conducted, the voltage output by the photosensitive tube is limited by the R15, so that the bidirectional trigger diode D3 is conducted, a trigger signal is provided for the G pole of the bidirectional thyristor T1, and the T1 is conducted. And after the T1 is conducted, the heater EH is powered to start heating the water mist.

The conduction level of T1 is also controlled by the leakage current of the EH. EH belongs to PTC type heater, and when the water smoke volume that the blast pipe discharged is big, the leakage current of EH also can increase, and the trigger voltage for T1 provides increases, and T1 switches on and strengthens, and the operating voltage for EH provides increases, makes the heating temperature of EH rise to make the water smoke temperature that the heater spun rise. Otherwise, the control process is reversed.

Thermal insulation coating: the three different box bodies are respectively provided with a heat insulation coating layer for ensuring no heat transfer between the box bodies and the outside. The heat insulation coating and the box body contact face use heat insulation silica gel, the heat insulation cotton is connected with the box body through the heat insulation silica gel, heat loss is reduced to the minimum, the temperature in each box body can be kept at a stable level independently, and the culture quality is improved.

And (4) water-free protection: the water-free protection is completed by the water level probe S3. After water is added, a water level switch S3 is switched on, and the oscillator and the heater can work; if the water level is too low, S3 is cut off, so that not only the Q5 is cut off to stop the oscillator and the transducer, but also the Q4 is cut off to stop the heater, thereby avoiding the damage of the transducer, the heater and other elements and realizing the waterless protection.

The MH-410D sensor detects CO2 by using an NDIR principle, and has the characteristics of analog voltage signal and serial port communication functions, high sensitivity, standard output and digital output.

The parameters of the MH-410D sensor are set as:

and (3) analog voltage output: vout output voltage range (0.4-2V) corresponding to gas concentration (0-full range). The Vin end of the sensor is connected with 5V, the GND end is connected with the power ground, and the Vout end is connected with the input end of the ADC. After the sensor is preheated for a period of time, a voltage value representing the concentration of the gas is output from the Vout end, and 0.4-2.0V represents the concentration value of the gas to be in a range of 0-full range. When the self-test finds a fault, the output voltage of the sensor is 0V.

Serial output (UART): the serial port output comprises hardware connection and software setting

The hardware connections connect Vin-GND-RXD-TXD of the sensor to 5V-GND-TXD-RXD, respectively. (the client must use TTL level). The detector can directly read the gas concentration value through the UART interface of the sensor without calculation.

Software sets the serial port baud rate to 9600, the data bit to 8 bits, the stop bit to 1 bit, and the parity bit to none. The list of protocol command interfaces and their meanings are shown in table 5.

TABLE 5 protocol Command interface List and meanings

In operation, pin 1 of MH-410D is connected to +5V, and pin 5 is connected to ground.

Pins

2 and 3 are respectively connected with TXD and RXD interfaces of the single chip microcomputer, the single chip microcomputer can directly read the gas concentration value through a UART interface of a sensor MH-410D without calculation, and then the measurement result is sent to an LED to display the current CO2 concentration;

the pin 4 is an analog voltage output pin, and outputs the representation CO after the preheating time₂Voltage value of concentration, voltage range 0.4-2V corresponds to CO₂The concentration is 0-5%, and the device is connected with a relay to control CO₂The device is ventilated, the user can set a multi-gear limit value through R4, when CO is detected₂When the concentration is higher than the set value, the pin 4 outputs high level to close CO₂The equipment otherwise outputs low level to drive CO₂Equipment; and when the fault occurs, the output voltage of the pin 4 is 0V, and the pin can be connected with a buzzer to monitor the working state. An anaerobic indicator is arranged in the box body. The definition specification table of MH-410D pin is shown in Table 6.

TABLE 6 MH-410D Pin definition description Table

CO₂The supply equipment consists of mixed gas (CO) connected in sequence by a gas pipeline₂:H₂:N₂1:1:8) steel cylinder 2, three-way valve 4, buffer bottle 5 and vacuum pump 6, wherein pressure gauge 3 is connected on the gas pipeline connected between steel cylinder 2 and three-way valve 4, and three-way valve 4 is connected with the box body through the gas pipeline. The vacuum pump 6 pumps out the air in the anaerobic incubator body, and when the air content is reduced, O in the anaerobic incubator body₂The concentration is also reduced, at which point O can be observed by an anaerobic indicator₂And (4) when the concentration meets the specified requirement, opening the three-way valve, introducing the mixed gas, and paying attention to slowly opening a valve of the mixed gas steel cylinder to keep the pressure in the anaerobic incubator consistent with the atmospheric pressure as far as possible.

The reset of the singlechip is realized by an external circuit. After the clock circuit works, as long as the high level of more than 24 clock oscillation pulses (namely 2 machine periods) appears on the complex (RST) pin of the single chip microcomputer, the single chip microcomputer realizes the reset of the initialization state. Therefore, in order to ensure that the single chip microcomputer can be reliably reset, in the circuit design of an application system, the RST pin is kept at a high level more than 10ns, and the single chip microcomputer 52 can be circulated to a reset state. When the single chip microcomputer is reset, namely RST is changed from high level to low level, the 52 single chip microcomputer starts to execute the program from 0000H address. The reset state table of the MCS-51 single-chip after reset is shown in Table 7.

Table 7 reset state table of MCS-51 single chip microcomputer after reset

After reset, the outputs of ports P0-P3 are high, and these quasi-bidirectional ports are all in input state, and 07H is written into stack pointer SP while program counter PC and other special function registers SFR are cleared 0, but reset does not affect the state of RAM inside the single chip.

The crystal oscillator provides working signal pulses for the single chip microcomputer, the pulses are the working speed of the single chip microcomputer (for example, 12M crystal oscillator, the working speed of the single chip microcomputer is 12M per second), the working frequency of the single chip microcomputer is within a range, and otherwise, the working state of the single chip microcomputer beyond the range is unstable.

The pins XTAL1 and XTAL2 of the single chip microcomputer are externally connected with a quartz crystal (crystal oscillator for short) and used as a load of an internal oscillation circuit of the single chip microcomputer to form a self-excited oscillator which can generate a clock pulse signal inside the single chip microcomputer. The C1 and C2 have the functions of stabilizing the oscillation frequency and starting oscillation quickly. According to the selection parameters of the classical circuit, the circuit selects a crystal oscillator with the frequency of 12MHz and the frequency of 33PF with the frequency of C1-C2. Where the crystal period (or the period of the external clock signal) is the smallest unit of timing.

For a complete electronic design, the primary problem is to provide a power supply module for the whole system, and the stability and reliability of the power supply module are the premise and the foundation of stable operation of the system. Although the 52 single chip microcomputer is early in service time and wide in application range, in the actual use process, one and typical problem is that the 52 single chip microcomputer is easy to interfere and generates a program runaway phenomenon, and an important means for overcoming the phenomenon is to configure a stable and reliable power supply module for a single chip microcomputer system. The power supply of the power supply module in the minimum system can be supplied through a USB port of the computer, and can also be supplied by an external stable 5V power supply module.

A method for acquiring and adjusting the temperature, humidity and carbon dioxide concentration in a multi-scene and multi-strain incubator in real time comprises the step of acquiring and adjusting culture environment parameters.

As shown in fig. 14, the method for acquiring three parameters of temperature, humidity and carbon dioxide concentration in the box body in real time through the single chip microcomputer comprises the following steps:

step 2), setting an initial value e (n-1) ═ e (n-2) ═ 0;

step 3) inputting y (n) for the current sampling;

step 4), calculating e (n) and △ u (n);

and 6) when the sampling time is reached, turning to the step 3) for next sampling.

As shown in fig. 15, the method for automatically controlling and adjusting three parameters of temperature, humidity and carbon dioxide concentration in the box in real time includes the following steps:

according to the obtained sampling information data, the single chip microcomputer queries a corresponding fuzzy control table according to a system error (deviation is a given value-a feedback value) and a deviation change rate (current period deviation-last period deviation) to obtain K_P，K_I，K_dAnd PID operation is carried out on the setting values of the three parameters.

The incremental PID is only related to the nearest n-2, n-1, n times of errors, and the single chip microcomputer only needs to store the errors for three times; and position formula PID: u (n) ═ K_pe(n)+K_i∑^k _i＝0e(i)+K_d[e(n)-e(n-1)]Errors need to be accumulated, and the burden of a single chip microcomputer is increased.

Incremental PID formula: u (n) ═ K_p(1+T/T_i+T_d/T)e_n-K_p(1+2T_d/T)e_n-1+K_p(T_d/T)e_n-2+ u (n-1), where u (n), u (n-1) are output values at sampling times n, n-1, respectively; k_p，T_i，T_dProportional, integral and differential coefficients, respectively; e.g. of the type_n，e_n-1，e_n-2At sampling times n, n-1, n-2, respectivelyThe deviation value of (a); t is the sampling period.

A＝K_p(1+T/T_i+T_d/T)；B＝K_p(1+2T_d/T)；C＝K_p(T_d/T)。

The multi-scene and multi-strain incubator provided by the invention has the advantages of scientific and reasonable design, simplicity and convenience in operation, and capability of adjusting the temperature, humidity and CO in the incubator body₂The concentration is accurately and stably automatically controlled and adjusted, various conditions of different strains in the growth environments of different boxes are maintained in the most appropriate range, the simultaneous culture of different strains and different environments can be realized, the culture success rate is high, and the requirements of practical application can be well met.

The above-mentioned embodiments only express the embodiments of the present invention, and the description thereof is more specific and detailed, but not construed as limiting the scope of the present invention. It should be noted that, for a person skilled in the art, several variations and modifications can be made without departing from the inventive concept, which falls within the scope of the present invention. Therefore, the protection scope of the present patent shall be subject to the appended claims.

Claims

1. The utility model provides an incubator of many scenes, many bacterial, its characterized in that, including three-in-one incubator body of aerobic fungus, anaerobe and fungi, singlechip, control module, display module, power supply circuit and 3 temperature and humidity sensors, 1 CO of setting in the box₂Sensor, heater, humidifier and CO₂And supplying the equipment.

2. The multi-scenario, multi-species incubator of claim 1, wherein the heater is a heating wire heater.

3. The multi-scenario, multi-strain incubator as claimed in claim 1, wherein the display module is an LED nixie tube display screen.

4. The multi-scenario, multi-bacterial incubator of claim 1, wherein the display module is an LED nixie tube display screen comprising 3 double 8-digit tubes.

5. The multi-scenario, multi-species incubator of claim 1, wherein the temperature and humidity sensor is STH 11.

6. The multi-scenario, multi-species incubator of claim 1, wherein the CO is₂The sensor is MH-410D.

7. The multi-scenario, multi-strain incubator as claimed in claim 1, wherein the single chip microcomputer is STC89C52 single chip microcomputer.

8. The multi-scenario, multi-species incubator as claimed in claim 1, wherein the temperature, humidity and CO in the chamber are adjusted₂The concentration is dynamically obtained and adjusted in real time;

the real-time acquisition comprises the following steps:

step 2), setting an initial value e (n-1) ═ e (n-2) ═ 0;

step 3) inputting y (n) for the current sampling;

step 4), calculating e (n) and △ u (n);

The adjustment comprises the following steps:

9. The multi-scenario and multi-species incubator as claimed in claim 1, wherein the inside of the three-in-one incubator body with aerobic bacteria, anaerobic bacteria and fungi is covered with a heat insulation layer made of heat insulation cotton and heat insulation silica gel, the heat insulation layer is covered with the heat insulation silica gel on the side contacting with the incubator body, and the heat insulation cotton is connected with the incubator body through the heat insulation silica gel.