CN110786957A - A small specific area soft tissue quantitative excision device - Google Patents

Abstract

The invention discloses a quantitative excision device for soft tissue in a tiny specific area, which comprises an injector body, an excision part and an adsorption part, wherein the fixed end of the adsorption part is fixed on the inner wall of a nipple of the injector body; the excision part is detachably connected with the outer wall of the nipple of the injector body through a connecting piece; the adsorption component is arranged in the connecting piece in a penetrating mode, and the free end of the adsorption component is located in the cutting component and matched with the cutting component. The invention overcomes the defect that the prior art can not carry out quantitative excision and adsorption of soft tissues in a specific region at millimeter level, realizes mechanized excision of soft tissues in the specific region, has good repeatability and little damage to residual tissues, and has the advantages of simple structure, convenient operation, low cost and the like, thereby having wide application prospect.

Description

A small specific area soft tissue quantitative excision device

technical field

The invention relates to the technical field of animal tissue excision, in particular to a quantitative excision device for soft tissue in a small specific area.

Background technique

Animal experimentation is an extremely important part in the field of life sciences. Animal experiments need to build standardized different models to meet the requirements of different experiments, and the production of animal models must be scientific, and scientific requires animal models to have certain standards, stability, and repeatability. At present, for the model making of soft tissue excision in a small specific area, the commonly used method is to use micro-cutting, but this excision method is only completed by personal touch, its stability is poor, and quantitative excision cannot be achieved, which seriously affects the The accuracy and repeatability of subsequent experiments hinder the development and progress of science.

SUMMARY OF THE INVENTION

The purpose of the present invention is to provide a quantitative excision device for soft tissue in a small specific area, which is used to solve the problems of poor stability and inability to perform quantitative excision in the existing micro-shearing.

The invention provides a quantitative excision device for soft tissue in a tiny specific area, comprising a syringe body, a cutting part and an adsorption part, the fixed end of the adsorption part is fixed on the inner wall of the nipple of the syringe body; the excision part is connected to the syringe body The outer walls of the nipples are detachably connected through a connecting piece; the suction part is penetrated in the connecting piece, and the free end of the suction part is located in the cutting part and fits with it.

Preferably, both ends of the connecting piece are respectively provided with a nipple outer wall collar and a resection head collar, and the nipple outer wall collar and the resection head collar are respectively sleeved on the outer wall of the nipple and the resection head of the syringe body. The middle part of the connecting piece is provided with a hollow part, and the free end of the adsorption part passes through the hollow part of the connecting piece and extends into the cutting head collar of the connecting piece.

Preferably, the connecting member is integrally formed with the cutting member.

Preferably, the adsorption component is an adsorption hose.

Preferably, the free end of the adsorption member is provided with an adsorption needle.

Preferably, the cutting member is a cutting head.

Preferably, the cutting head is a metal slice.

The beneficial effects of the present invention are:

On the basis of the existing common syringe, the quantitative resection device for soft tissue in a small specific area disclosed in the present invention is not only suitable for general biological soft tissue resection, but also for living or isolated tissue by adding a resection part and an adsorption part. Animal brain tissue, spinal cord tissue, muscle tissue, etc. In practical applications, it can be used in combination with the existing stereotaxic instrument to achieve the effect of accurately positioning a specific area for quantitative excision, thereby providing a reliable and repeatable animal model for subsequent experiments. The present invention overcomes the disadvantage that soft tissue quantitative excision and adsorption cannot be performed in a specific area of millimeter level in the prior art, realizes mechanized soft tissue excision in specific area, has good repeatability, little damage to remaining tissue, and has the advantages of simple structure, convenient operation, Low cost and other advantages, the application prospect is broad.

Description of drawings

1 is a schematic structural diagram of a device provided by the present invention;

FIG. 2 is a schematic diagram of a use state of the device provided by the present invention.

Detailed ways

The following examples are used to illustrate the present invention, but are not intended to limit the protection scope of the present invention.

In the description of the embodiments of the present invention, it should be noted that the terms "left" and "right" are based on the azimuth or positional relationship described in the drawings, which are for the convenience of describing the present invention. The terms "first", "second", etc. are used for descriptive purposes and should not be understood as indicating or implying relative importance or implying the number of indicated technical features.

Example 1

Embodiment 1 provides a quantitative soft tissue excision device in a small specific area, the structure of which will be described in detail below.

Before describing this device for quantitative resection of soft tissue in small specific areas, it is necessary to introduce a common syringe.

The structure of an ordinary syringe generally includes a nipple, an empty barrel (including a scale line), a piston, a piston shaft, and a piston handle. In addition, a needle is also included, and the structure of the needle includes a needle tip, a needle stem, and a needle plug.

Referring to FIG. 1, the device for quantitatively excising soft tissue in a small specific area includes a syringe body 1, a cutting part 2 and an adsorption part 3, and the fixed end of the adsorption part 3 is fixed on the inner wall of the nipple 10 of the syringe body 1; the excision part 2 and the outer wall of the nipple 10 of the syringe body 1 are detachably connected through the connecting piece 5; inside and fit into Part 2.

Wherein, both ends of the connecting piece 5 are respectively provided with a nipple outer wall collar 51 and a resection head collar 52 , and the nipple outer wall collar 51 and the resection head collar 52 are respectively sleeved on the nipple 10 of the syringe body 1 . The outer wall of the connecting piece 5 and the cutting part 2; the middle part of the connecting piece 5 is provided with a hollow part 53; Resection head collar 52 inside.

In the above embodiment, the connecting member 5 and the cutting member 2 are integrally formed.

In the above embodiment, the adsorption member 3 is an adsorption hose. Preferably, the free end of the adsorption part 3 is provided with an adsorption needle.

In the above embodiment, the cutting member 2 is a cutting head. Preferably, the cutting head is a metal slice. Among them, metal slices can be prepared by selecting corresponding materials according to the hardness of the resected tissue, and the metal slices can be replaced with different shapes, thereby realizing quantitative cutting of soft tissues in different regions and shapes. After the cutting is completed, the cut soft tissue is suctioned and removed by the suction member 3 connected to the nipple 10 of the syringe body 1 . The adsorption part 3 can be removed through the hollow part 53 of the connector 5. If the space permits, it can be removed, and the adsorption needle can be inserted into the cutting area to absorb the remaining soft tissue, so that the incision is more complete and no residue is left.

The adsorption part 3 is slightly lower than the surface of the soft tissue according to the cutting depth. After the cutting part 2 cuts the soft tissue, the adsorption part 3 is basically attached to the surface of the soft tissue, and then the cut soft tissue is absorbed by the adsorption part 3 . If it is necessary to further remove the unsucked soft tissue around the resection part 2, the adsorption part 3 can be removed, the resection part 2 can be inserted into the same depth, and a small amount of remaining soft tissue can be sucked out with a syringe needle.

In practical applications, the present invention can be used in combination with an existing stereotaxic instrument, which can achieve the effect of accurately positioning a specific area for quantitative excision, thereby providing a reliable and repeatable animal model for subsequent experiments. Therefore, the present invention will have broad application prospects in quantitative excision of target tissue. Taking the brain of a mouse as an example, referring to Figure 2, quantitative resection of necrotic brain tissue in a specific area after stroke is achieved.

Before the experiment, the anesthetized mice were fixed on the platform of the stereotaxic instrument, and the head of the mouse was fixed by the incisor clips and ear bars on the stereotaxic instrument. The syringe body 1 is clamped on the vertical fixing rod 9 of the stereotaxic instrument 8 .

The head skin was prepared, and a median incision was taken, the skin and subcutaneous tissue were incised, and separated until the skull was exposed. According to the mouse stereotaxic map, the stroke injury area was located, and the six points were: bregma+2.0mm/right lateral opening 1.6mm, bregma+2.0mm/right lateral opening 3.2mm, bregma 0/right lateral opening 1.6 mm mm, bregma 0/right lateral opening 4.0mm, bregma-2.0mm/right lateral opening 2.0, bregma-2.0/right lateral opening 4.0. Connect the 6 points respectively, grind the hexagonal bone window along the connecting line with a dental drill, remove the bone flap, and peel off the dura. Place the syringe body 1 just above the bone window, make the vertical rod of the stereotaxic instrument drive the present invention to slide down, observe the descending position of the excision part 2 under a microscope, and stop rotating the height of the stereotaxic instrument when the excision part 2 contacts the brain tissue. Control the button, slightly adjust the front, back, left and right of the vertical rod to align the metal slice with the edge of the bone window, read the height value at this time, and then continue to rotate the button according to the volume of tissue to be removed to adjust the insertion depth of the resection part 2 until reach the desired height. Taking the excision of the injured cortex in mice as an example, drop 1mm to separate the necrotic tissue from the surrounding area, and use a piston to suck out the necrotic brain tissue through the hose. After removing large pieces of necrotic brain tissue, remove the piston hose and use a syringe needle. Aspirate the remaining brain tissue in the area surrounding the metal section to complete the entire excision process.

Although the present invention has been described in detail above with general description and specific embodiments, some modifications or improvements can be made on the basis of the present invention, which will be obvious to those skilled in the art. Therefore, these modifications or improvements made without departing from the spirit of the present invention fall within the scope of the claimed protection of the present invention.

Claims (7)

1. a small specific area soft tissue quantitative excision device is characterized in that, comprises syringe body (1), excision part (2) and adsorption part (3), The fixed end of the adsorption part (3) is fixed on the inner wall of the nipple (10) of the syringe body (1); The cutting part (2) is detachably connected with the outer wall of the nipple (10) of the syringe body (1) through a connecting piece (5); The adsorption part (3) is penetrated in the connecting piece (5), and the free end of the adsorption part (3) is located in the cutting part (2) and fits with it. 2. The device for quantitatively excising soft tissue in tiny specific areas as claimed in claim 1, characterized in that, Both ends of the connecting piece (5) are respectively provided with a nipple outer wall collar (51) and a resection head collar (52), The nipple outer wall collar (51) and the cutting head collar (52) are respectively sleeved on the outer wall of the nipple (10) of the syringe body (1) and the cutting part (2); The middle part of the connecting piece (5) is provided with a hollow part (53), The free end of the suction part (3) passes through the hollow part (53) of the connecting piece (5) and extends into the cutting head collar (52) of the connecting piece (5). 3. The device for quantitatively excising soft tissue in tiny specific areas as claimed in claim 1, characterized in that, The connecting piece (5) is integrally formed with the cutting part (2). 4. The device for quantitatively excising soft tissue in tiny specific areas according to claim 1, wherein, The adsorption component (3) is an adsorption hose. 5. The device for quantitatively excising soft tissue in tiny specific areas as claimed in claim 1, characterized in that, The free end of the adsorption part (3) is provided with an adsorption needle. 6. The device for quantitatively excising soft tissue in tiny specific areas according to claim 1, wherein, The cutting part (2) is a cutting head. 7. The device for quantitatively excising soft tissue in tiny specific areas as claimed in claim 5, characterized in that, The cutting head is a metal slice.

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