CN110771511A - Method for open culture of banana tissue culture seedlings - Google Patents
Method for open culture of banana tissue culture seedlings Download PDFInfo
- Publication number
- CN110771511A CN110771511A CN201911177574.5A CN201911177574A CN110771511A CN 110771511 A CN110771511 A CN 110771511A CN 201911177574 A CN201911177574 A CN 201911177574A CN 110771511 A CN110771511 A CN 110771511A
- Authority
- CN
- China
- Prior art keywords
- culture
- seedlings
- bottle
- tissue culture
- banana
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Pending
Links
Classifications
-
- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01H—NEW PLANTS OR NON-TRANSGENIC PROCESSES FOR OBTAINING THEM; PLANT REPRODUCTION BY TISSUE CULTURE TECHNIQUES
- A01H4/00—Plant reproduction by tissue culture techniques ; Tissue culture techniques therefor
- A01H4/008—Methods for regeneration to complete plants
-
- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01H—NEW PLANTS OR NON-TRANSGENIC PROCESSES FOR OBTAINING THEM; PLANT REPRODUCTION BY TISSUE CULTURE TECHNIQUES
- A01H4/00—Plant reproduction by tissue culture techniques ; Tissue culture techniques therefor
- A01H4/001—Culture apparatus for tissue culture
Landscapes
- Life Sciences & Earth Sciences (AREA)
- Developmental Biology & Embryology (AREA)
- Engineering & Computer Science (AREA)
- Biotechnology (AREA)
- Cell Biology (AREA)
- Botany (AREA)
- Environmental Sciences (AREA)
- Breeding Of Plants And Reproduction By Means Of Culturing (AREA)
Abstract
The invention relates to a plant seedling cultivation method, in particular to a method for open cultivation of banana tissue culture seedlings, and belongs to the technical field of biology. The method for open culture of banana tissue culture seedlings comprises the steps of disinfection of a culture bottle, preparation of a culture medium, inoculation operation, induced culture of the tissue culture seedlings, multiplication culture and rooting culture. The method is simple to operate, and the volcanic rock powder and the antibacterial agent are added into the boiled culture medium, are uniformly stirred, are subpackaged and are cooled for use; the inoculation and the culture are not required to be carried out under the aseptic condition, the working efficiency is greatly improved, and the cost is favorably reduced; high-temperature and high-pressure sterilization pot equipment is not needed, various complicated procedures and potential safety hazards caused by the application of special equipment are avoided, the production cost is reduced, and the popularization and the application of the banana tissue culture seedlings are facilitated.
Description
Technical Field
The invention relates to a plant seedling cultivation method, in particular to a method for open cultivation of banana tissue culture seedlings, and belongs to the technical field of biology.
Background
Bananas (Musa spp.) are one of four good fruits in south China, and are also famous tropical and subtropical fruits in the world. Bananas are also considered by the food and agriculture organization of the united nations as the fourth most important food crop next to rice, wheat, corn, in some developing countries and regions as the main food for local farmers.
Most edible bananas cultivated are bred by bud picking, and after long-term use, the edible bananas are easy to carry various pathogenic bacteria such as mosaic virus, fasciola virus, panama pathogen and the like, and the pathogenic bacteria are parasitic in a banana vascular bundle system, influence the transportation of moisture and nutrients and photosynthesis and cause serious harm to the production and cultivation of the bananas. Tissue culture is a new technology for rapidly propagating banana seedlings, which is researched and developed in the later 80 th century, and the technology can not only rapidly propagate the banana seedlings, but also remove viruses carried by bananas and provide high-quality seedlings for banana production, so that the technology is rapidly popularized and applied in banana industry.
Traditional tissue culture requires an aseptic environment, requires that the inoculation process and the culture process are carried out in the aseptic environment, so that the labor cost, the facility cost, the culture medium cost, the power supply cost and the like are greatly increased, moreover, bananas belong to low-value agricultural products, the input cost is too high, and the popularization and application of the technology in the banana industry are greatly limited.
If the antibacterial agent is added into the culture medium, the complicated operation steps of the technology can be simplified, the pollution problem in the tissue culture process can be effectively controlled, and the normal growth of plant tissues is ensured. The culture medium does not need high-temperature and high-pressure sterilization and sterile operation, so that the production cost of banana tissue culture and the requirement on special equipment such as a high-temperature and high-pressure sterilization pot can be reduced, and the input cost can be greatly reduced.
The antibacterial agent is added into the culture medium, so that the production cost of tissue culture and the requirements on special equipment such as a high-temperature and high-pressure sterilization pot and the like can be reduced, but the growth of the tissue culture seedlings is influenced to a certain degree, such as blackening of a basal part, browning, difficulty in rooting and the like. The volcanic rock is a porous rock formed after volcanic eruption, contains dozens of mineral substances such as sodium, magnesium, calcium and the like and trace elements, has no radiation and far infrared magnetic waves, and has the effects of stabilizing water quality (peracid or alkali water can be properly adjusted to be close to neutral), adsorbing harmful substances, killing bacteria and the like. The volcanic rock powder is added into the culture medium of the open tissue culture of bananas, so that the influence on the growth of the banana tissue culture seedlings caused by the addition of an antibacterial substance can be avoided, the pH value in the culture medium can be adjusted at the later growth stage of the banana tissue culture seedlings, and the culture medium also has certain effects of sterilizing and promoting the growth of the banana tissue culture seedlings.
Disclosure of Invention
The invention aims to provide a method for open culture of banana tissue culture seedlings, which aims to solve the problems in the background art.
A method for open culture of banana tissue culture seedlings comprises the following steps:
(1) and (3) sterilizing the culture bottle: and cleaning the culture bottle with tap water, soaking the culture bottle with 0.1% sodium hypochlorite, wherein the liquid level of the soaking is higher than the bottle opening by more than 3cm, pressing the matched plastic bottle cover with a heavy object, and completely soaking for 2-3 h. After soaking, the culture bottle is fished out, the bottle mouth is downwards buckled and dried on the tissue culture basket, the cover is fished out and placed in the basket capable of filtering water, and the water is filtered.
(2) Preparation of a culture medium: preparing a basic culture medium according to a preparation method of a conventional culture medium, wherein the basic culture medium is MS + 0.3% of cane sugar +4.8g/L of carrageenan +0.2g/L of volcanic rock powder, adding different types of hormones and concentrations according to requirements of different growth periods of banana tissue culture, adding water to a constant volume, covering a cover to boil for 2min, adjusting the pH to 5.8-6.0, adding 0.1% of carbendazim antibacterial agent, uniformly stirring, subpackaging and cooling for later use.
(3) And (3) inoculation operation: and (3) sterilizing the inoculation chamber by irradiating with an ultraviolet lamp for 20-30 min, cleaning the inoculated table, wiping with 75% alcohol for sterilization, and sterilizing the inoculated instrument by using an infrared ray inoculation sterilizer.
(4) And (3) induction culture: taking the strong banana sucker without diseases and insect pests as an explant, removing surface soil, drying in the air, and cutting into stem tips with the size of 6cm multiplied by 8 cm; soaking and disinfecting the stem tip with alcohol for 15min, washing the stem tip with sterile water for 5-6 times, and cutting the stem tip into stem tips with the size of 3cm multiplied by 5 cm; the method comprises the steps of shaking and sterilizing for 8min by using 2% sodium hypochlorite, washing for 5-6 times by using sterile water, cutting into stem tips with the size of 1cm multiplied by 1cm, and inoculating MS +4mg/L6-BA + 0.3% of cane sugar +4.8g/L carrageenan +0.2g/L of volcanic rock powder + 0.1-0.45% of antibacterial agent into a culture medium. The temperature of the culture room is 27 +/-1 ℃, the daily illumination is 10h, the illumination intensity in the first 7 days is 500-.
(5) And (3) proliferation culture: after induction culture and growth of cluster buds, cutting the cluster buds according to 2-3 strains, inoculating the cut cluster buds into a multiplication culture medium, wherein the multiplication culture medium comprises MS +3mg/L6-BA +0.1mg/LNAA + 0.3% of cane sugar +4.8g/L carrageenan +0.2g/L of volcanic rock powder + 0.1-0.45% of antibacterial agent, and transferring once in 20 days. The temperature of the culture room is 27 +/-1 ℃, dark culture is carried out for the first 7 days, then illumination is carried out for 10 hours every day, the illumination intensity is 1000-.
(6) Rooting culture: when the height of the seedling is not less than 3cm, the seedling is cut into single plants, then the single plants are transferred into a rooting culture medium of 1/2MS +0.1mg/L NAA + 0.3% of cane sugar +4.8g/L of carrageenan + 0.1% -0.45% of antibacterial agent, and the seedlings are cultured for 20-30 days and then can be transplanted. The temperature of the culture room is 27 +/-1 ℃, the daily illumination is 12 hours, the illumination intensity is 2000-.
Preferably, 0.2g/L of volcanic rock powder is added into each culture medium.
Preferably, the antibacterial agent used in each culture medium is carbendazim.
Preferably, the concentration of the carbendazim in each culture medium is 0.1-0.45%.
Preferably, the culture media do not need autoclaving, the bacteriostat is added after boiling and is uniformly stirred, and the culture media can be used after being subpackaged, cooled and solidified.
Preferably, an ultra-clean workbench is not needed in the inoculation operation, the inoculation chamber is irradiated by an ultraviolet lamp for sterilization for 20-30 min, the inoculation chamber is wiped on a clean bench by 75% alcohol for sterilization, and the inoculated instruments are sterilized by an infrared inoculation sterilizer.
The research of the invention finds that 0.25 percent of carbendazim has the best antibacterial effect on the basis of adding 0.2g/L of volcanic rock powder into a culture medium, the pollution condition can not occur, and the growth of tissue culture seedlings is the same as that of conventional tissue culture; the 0.1 percent of carbendazim has the next grade, although the growth of the banana tissue culture seedlings is not influenced, the pollution rate is 6.7 percent; the carbendazim with the concentration of 0.35 percent and 0.45 percent has good antibacterial effect, but can affect the growth of the tissue culture seedlings of the bananas, and is not suitable for open culture of the tissue culture seedlings of the bananas.
Compared with the traditional banana tissue culture, the method has the following beneficial effects:
1. the method is simple to operate, and the method can be used only by adding the antibacterial agent into the boiled culture medium, uniformly stirring, subpackaging and cooling.
2. The volcanic stone powder is added in the culture medium, which is beneficial to the growth of the tissue culture seedlings of bananas.
3. The inoculation and the culture of the invention do not need to be carried out under the aseptic condition, the working efficiency is greatly improved, and the production cost is favorably reduced.
4. The invention does not need high-temperature high-pressure sterilization pot equipment, not only avoids various complicated procedures and potential safety hazards caused by the application of special equipment, but also reduces the production cost, and is beneficial to the popularization and application of the banana tissue culture seedlings.
Detailed Description
The invention is further illustrated by the following examples.
Example 1
A method for open culture of banana tissue culture seedlings comprises the following steps:
1. and (3) sterilizing the culture bottle: and cleaning the culture bottle with tap water, soaking the culture bottle with 0.1% sodium hypochlorite, wherein the liquid level of the soaking is higher than the bottle opening by more than 3cm, pressing the matched plastic bottle cover with a heavy object, and completely soaking for 2-3 h. After soaking, the culture bottle is fished out, the bottle mouth is downwards buckled and dried on the tissue culture basket, the cover is fished out and placed in the basket capable of filtering water, and the water is filtered.
2. Preparation of a culture medium: preparing a basic culture medium according to a preparation method of a conventional culture medium, wherein the basic culture medium is MS + 0.3% of cane sugar +4.8g/L of carrageenan +0.2g/L of volcanic rock powder, adding different hormones and concentrations according to the requirements of different growth periods of banana tissue culture, adding water to a constant volume, covering a cover to boil for 2min, adjusting the pH to 5.8-6.0, adding 0.1% of carbendazim antibacterial agent, uniformly stirring, subpackaging and cooling for later use.
3. And (3) inoculation operation: and (3) sterilizing the inoculation chamber by irradiating with an ultraviolet lamp for 20-30 min, cleaning the inoculated table, wiping with 75% alcohol for sterilization, and sterilizing the inoculated instrument by using an infrared ray inoculation sterilizer.
4. And (3) induction culture: taking the strong banana sucker without diseases and insect pests as an explant, removing surface soil, drying in the air, and cutting into stem tips with the size of 6cm multiplied by 8 cm; soaking and disinfecting the stem tip with alcohol for 15min, washing the stem tip with sterile water for 5-6 times, and cutting the stem tip into stem tips with the size of 3cm multiplied by 5 cm; the method comprises the steps of shaking and sterilizing for 8min by using 2% sodium hypochlorite, washing for 5-6 times by using sterile water, cutting into stem tips with the size of 1cm multiplied by 1cm, and inoculating MS +4mg/L6-BA + 0.3% of cane sugar +4.8g/L carrageenan +0.2g/L of volcanic rock powder + 0.1-0.45% of antibacterial agent into a culture medium. The temperature of the culture chamber is 27 +/-1 ℃, the illumination time per eye is 10 hours, the illumination intensity in the first 7 days is 500-1500 lx, the illumination intensity in the later days is 1000-1500lx, and the air humidity is 60-70%.
5. And (3) proliferation culture: after induction culture and growth of cluster buds, cutting the cluster buds according to 2-3 strains, inoculating the cut cluster buds into a multiplication culture medium, wherein the multiplication culture medium comprises MS, 3mg/L6-BA, 0.1mg/L NAA, 0.3% of cane sugar, 4.8g/L carrageenan, 0.2g/L of volcanic powder and 0.1% of carbendazim antibacterial agent, and transferring once in 20 days. The temperature of the culture room is 27 +/-1 ℃, dark culture is carried out for the first 7 days, then illumination is carried out for 10 hours every day, the illumination intensity is 1000-.
6. Rooting culture: and when the height of the seedlings is not less than 3cm, cutting the seedlings into single plants, transferring the single plants into a rooting culture medium of 1/2MS, NAA0.1mg/L, 0.3% of cane sugar, 4.8g/L of carrageenan, 0.2g/L of volcanic rock powder and 0.1% of carbendazim antibacterial agent, and culturing for 20-30 days to obtain the seedlings which can be transplanted. The temperature of the culture room is 27 +/-1 ℃, the daily illumination is 12 hours, the illumination intensity is 2000-.
Example 2
A method for open culture of banana tissue culture seedlings comprises the following steps:
the operation of this embodiment is the same as that of embodiment 1 except for the following operation, which is not described again.
The concentration of the carbendazim antibacterial agent added to each medium was 0.25%.
Example 3
A method for open culture of banana tissue culture seedlings comprises the following steps:
the operation of this embodiment is the same as that of embodiment 1 except for the following operation, which is not described again.
The concentration of the carbendazim antibacterial agent added to each culture medium was 0.35%.
Example 4
A method for open culture of banana tissue culture seedlings comprises the following steps:
the operation of this embodiment is the same as that of embodiment 1 except for the following operation, which is not described again.
The concentration of the carbendazim antibacterial agent added to each culture medium was 0.45%.
Comparative example 1
1. And (3) sterilizing the culture bottle: after the culture bottle is cleaned by tap water, the bottle mouth is downwards buckled on the tissue culture basket to be dried, and the cover is fished up and placed in the basket capable of filtering water to filter the water.
2. Preparation of a culture medium: preparing a basic culture medium according to a preparation method of a conventional culture medium, wherein the basic culture medium is MS + 0.3% sucrose +4.8g/L carrageenan, adding different hormones and concentrations according to the requirements of different growth periods of banana tissue culture, adding water to a constant volume, covering a cover, boiling for 2min, adjusting the pH to 5.8-6.0, subpackaging, and cooling for later use.
3. And (3) inoculation operation: and (3) sterilizing the inoculation chamber and the ultra-clean workbench by ultraviolet lamp irradiation for 20-30 min, wherein the inoculation process is carried out on the ultra-clean workbench, and the inoculated instruments are sterilized by an infrared inoculation sterilizer.
4. And (3) induction culture: taking the strong banana sucker without diseases and insect pests as an explant, removing surface soil, drying in the air, and cutting into stem tips with the size of 6cm multiplied by 8 cm; soaking and disinfecting the stem tip with alcohol for 15min, washing the stem tip with sterile water for 5-6 times, and cutting the stem tip into stem tips with the size of 3cm multiplied by 5 cm; the method comprises the steps of shaking and sterilizing for 8min by using 2% sodium hypochlorite, washing for 5-6 times by using sterile water, cutting into stem tips with the size of 1cm multiplied by 1cm, and inoculating MS +4mg/L6-BA + 0.3% of cane sugar +4.8g/L carrageenan +0.2g/L of volcanic rock powder + 0.1-0.45% of antibacterial agent into a culture medium. The temperature of the culture chamber is 27 +/-1 ℃, the illumination time per eye is 10 hours, the illumination intensity in the first 7 days is 500-1500 lx, the illumination intensity in the later days is 1000-1500lx, and the air humidity is 60-70%.
5. And (3) proliferation culture: after induction culture and growth of cluster buds, cutting the cluster buds according to 2-3 strains, inoculating the cut cluster buds into a multiplication culture medium, wherein the multiplication culture medium is MS +6-BA 3mg/L + NAA0.1mg/L + 0.3% sucrose +4.8g/L carrageenan, and transferring once in 20 days. The temperature of the culture room is 27 +/-1 ℃, dark culture is carried out for the first 7 days, then illumination is carried out for 10 hours every day, the illumination intensity is 1000-.
6. Rooting culture: and when the height of the seedling is not less than 3cm, cutting the seedling into single plants, transferring the single plants into a rooting culture medium of 1/2MS, NAA0.1mg/L, 0.3% of cane sugar and 4.8g/L of carrageenan, and culturing for 20-30 days to obtain the seedlings for transplanting. The temperature of the culture room is 27 +/-1 ℃, the daily illumination is 12 hours, the illumination intensity is 2000-.
Comparative example 2
The operation of this example is the same as that of comparative example 1 except that the following operation is different, and is not described in detail.
And (3) heating and dissolving the culture medium prepared in the step 2 without boiling, fixing the volume, adjusting the pH to 5.8-6.0, subpackaging, and sterilizing at high temperature and high pressure.
Comparative example 3
The operation of this embodiment is the same as that of embodiment 2 except for the following operation, which is not described again.
And 2, when the culture medium is prepared in the step 2, 0.2g/L of volcanic rock powder is not added.
TABLE 1 proliferation and rooting of tissue culture seedlings of bananas by open culture
In the open tissue culture of bananas, the carbendazim antibacterial agent is added, so that pollution can be effectively prevented, the using effect of the carbendazim antibacterial agent matched with volcanic rock powder is better, pollution can be better prevented, and the growth of tissue culture seedlings of bananas can be promoted.
The foregoing embodiments and description have been presented only to illustrate the principles and preferred embodiments of the invention, and various changes and modifications may be made therein without departing from the spirit and scope of the invention as hereinafter claimed.
Claims (7)
1. A method for open culture of banana tissue culture seedlings comprises the following steps:
(1) and (3) sterilizing the culture bottle: cleaning a culture bottle with tap water, soaking the culture bottle with 0.1% sodium hypochlorite, wherein the liquid level of the soaking is higher than the bottle opening by more than 3cm, pressing a matched plastic bottle cover with a heavy object, and completely soaking for 2-3 h; after soaking, fishing out the culture bottle, reversely buckling the bottle mouth downwards on a tissue culture basket, drying, fishing out a cover, putting the cover in the basket capable of filtering water, and filtering out the water;
(2) preparation of a culture medium: the basic culture medium is prepared according to the following formula: MS + 0.3% of cane sugar +4.8g/L of carrageenan +0.2g/L of volcanic rock powder, adding different hormones and concentrations according to the requirements of different growth periods of banana tissue culture, adding water to a constant volume, covering a cover to boil for 2min, adjusting the pH to 5.8-6.0, adding 0.1-0.45% of carbendazim antibacterial agent, stirring uniformly, subpackaging and cooling for later use;
(3) and (3) inoculation operation: sterilizing an inoculation chamber by using an ultraviolet lamp for 20-30 min, cleaning an inoculated table, wiping the table with 75% alcohol for sterilization, and sterilizing inoculated instruments by using an infrared ray inoculation sterilizer;
(4) and (3) induction culture: taking the strong banana sucker without diseases and insect pests as an explant, removing surface soil, drying in the air, and cutting into stem tips with the size of 6cm multiplied by 8 cm; soaking and disinfecting the stem tip with alcohol for 15min, washing the stem tip with sterile water for 5-6 times, and cutting the stem tip into stem tips with the size of 3cm multiplied by 5 cm; shaking and sterilizing with 2% sodium hypochlorite for 8min, washing with sterile water for 5-6 times, cutting into stem tips with the size of 1cm multiplied by 1cm, and inoculating the stem tips into a culture medium of MS +4mg/L6-BA + 0.3% of cane sugar +4.8g/L carrageenan +0.2g/L volcanic rock powder + 0.1-0.45% of antibacterial agent; the temperature of the culture chamber is 27 +/-1 ℃, the illumination time of each eye is 10 hours, the illumination intensity of the first 7 days is 500-1500 lx, the illumination intensity of the later days is 1000-1500lx, and the air humidity is 60-70 percent;
(5) and (3) proliferation culture: after induction culture and growth of cluster buds, cutting the cluster buds according to 2-3 strains, inoculating the cut cluster buds into a multiplication culture medium, wherein the multiplication culture medium comprises MS, 3mg/L6-BA, 0.1mg/L NAA, 0.3% of cane sugar, 4.8g/L carrageenan, 0.2g/L of volcanic rock powder and 0.1-0.45% of an antibacterial agent, and transferring once in 20 days; the temperature of the culture room is 27 +/-1 ℃, dark culture is carried out for the first 7 days, then illumination is carried out for 10 hours every day, the illumination intensity is 1000-;
(6) rooting culture: when the height of the seedlings is not less than 3cm, cutting the seedlings into single plants, transferring the single plants into a rooting culture medium of 1/2MS +0.1mg/L NAA + 0.3% of cane sugar +4.8g/L of carrageenan +0.2g/L of volcanic rock powder + 0.1-0.45% of antibacterial agent, and culturing for 20-30 days to obtain transplantable seedlings; the temperature of the culture room is 27 +/-1 ℃, the daily illumination is 12 hours, the illumination intensity is 2000-.
2. The method for open culture of banana tissue culture seedlings according to claim 1, wherein the culture bottles and bottle caps cleaned with tap water are soaked with 0.1% sodium hypochlorite, the liquid level of the soaking is higher than the bottle mouth by more than 3cm, the matched plastic bottle caps are pressed by a weight and completely soaked for 2-3 h; after soaking, the culture bottle is fished up, the bottle mouth is downwards buckled and dried on the tissue culture basket, and the bottle cap is fished up and placed in the basket capable of filtering water to filter out the water.
3. The method for open culture of banana tissue culture seedlings according to claim 1, wherein the method comprises the following steps: 0.2g/L of volcanic rock powder is added into each culture medium.
4. The method for open culture of banana tissue culture seedlings according to claim 1, wherein the method comprises the following steps: the antimicrobial used in each medium was carbendazim.
5. The method for open culture of banana tissue culture seedlings according to claim 2, characterized in that the concentration of carbendazim is 0.1% -0.45%.
6. The method for open culture of banana tissue culture seedlings according to claim 1, wherein the method comprises the following steps: the culture medium used for induction culture, proliferation culture and rooting culture does not need autoclaving, the bacteriostatic agent is added after boiling, the mixture is uniformly stirred, and the mixture is subpackaged, cooled and solidified for use.
7. The method for open culture of banana tissue culture seedlings according to claim 1, wherein the method comprises the following steps: the inoculation operation does not need a super-clean workbench, the inoculation chamber is irradiated by an ultraviolet lamp for disinfection for 20-30 min, the inoculation chamber is wiped on the clean workbench by 75% alcohol for disinfection, and the inoculated instruments are sterilized by an infrared inoculation sterilizer.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201911177574.5A CN110771511A (en) | 2019-11-26 | 2019-11-26 | Method for open culture of banana tissue culture seedlings |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201911177574.5A CN110771511A (en) | 2019-11-26 | 2019-11-26 | Method for open culture of banana tissue culture seedlings |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| CN110771511A true CN110771511A (en) | 2020-02-11 |
Family
ID=69392617
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN201911177574.5A Pending CN110771511A (en) | 2019-11-26 | 2019-11-26 | Method for open culture of banana tissue culture seedlings |
Country Status (1)
| Country | Link |
|---|---|
| CN (1) | CN110771511A (en) |
Cited By (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN113678737A (en) * | 2021-10-11 | 2021-11-23 | 浙江省亚热带作物研究所 | Open tissue culture method for polygonatum cyrtonema |
Citations (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN104609935A (en) * | 2014-12-18 | 2015-05-13 | 安徽豪景现代农业科技有限公司 | Pachira macrocarpa culture matrix |
| CN104885945A (en) * | 2015-05-26 | 2015-09-09 | 福建农林大学 | Chemical disinfection tissue culture method for Musa paradisiaca |
-
2019
- 2019-11-26 CN CN201911177574.5A patent/CN110771511A/en active Pending
Patent Citations (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN104609935A (en) * | 2014-12-18 | 2015-05-13 | 安徽豪景现代农业科技有限公司 | Pachira macrocarpa culture matrix |
| CN104885945A (en) * | 2015-05-26 | 2015-09-09 | 福建农林大学 | Chemical disinfection tissue culture method for Musa paradisiaca |
Non-Patent Citations (3)
| Title |
|---|
| 单芹丽等: "香蕉组培苗的生产技术", 《北方园艺》 * |
| 李子红等: "《珍品兰花快速繁殖与养护》", 30 November 2016 * |
| 解辉等: "次氯酸钠在香蕉开放式组织培养中的应用研究", 《热带作物学报》 * |
Cited By (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN113678737A (en) * | 2021-10-11 | 2021-11-23 | 浙江省亚热带作物研究所 | Open tissue culture method for polygonatum cyrtonema |
Similar Documents
| Publication | Publication Date | Title |
|---|---|---|
| Mahmoud et al. | Effect of different sterilization methods on contamination and viability of nodal segments of Cestrum nocturnum L | |
| CN105638477A (en) | Rapid propagation method for dendrobium hancockii seeds | |
| KR101453903B1 (en) | Production of Virus Free Plants from in Vitro Shoot Tips through in Vitro Meristem Culture | |
| CN103461134A (en) | Method for cultivating water lily aseptic seedling | |
| CN103688865B (en) | Inducing medium and method for improving survival rate of butterfly orchid pedicel | |
| CN104855292B (en) | A kind of method of Cinnamomum kanahirai hay stem segment tissue culture fast breeding | |
| CN105557518A (en) | Open type tissue culture and propagation method for rhizoma bletillae seeds | |
| CN102860259A (en) | High-efficiency industrial production method of virus-free tissue culture seedling of sweet potato | |
| CN106212281B (en) | A kind of method for tissue culture for improving banana survival rate | |
| CN105191792B (en) | The rapid propagation method of almond ringdove chrysanthemum | |
| CN114885837B (en) | Hormone-free culture method for citrus stem tip | |
| CN106258993B (en) | A kind of blueberry tissue culture method | |
| CN107135945B (en) | Tissue culture medium of linden tree and rapid propagation method thereof | |
| CN110771511A (en) | Method for open culture of banana tissue culture seedlings | |
| CN102524073B (en) | Plant tissue culture medium added with sodium hypochlorite for sterilization instead of high temperature and culture method | |
| CN106817949A (en) | A kind of Salvia japonica method for treating seeds | |
| CN107333657B (en) | A kind of North America Acer palmatum ' Atropurpureum' radiance in October kind tissue culture and rapid propagation method | |
| CN106718867A (en) | A kind of method that tomato tissue culture is carried out in test tube | |
| CN104782493A (en) | Paeonia ostii tissue culture method | |
| CN109496706A (en) | A kind of sterile brachyplast of Mao Xu Cao quickly breed before explant preprocess method | |
| CN105432471B (en) | The rapid propagation method of sansevieria trifasciata prain bud callus seedling | |
| KR101064947B1 (en) | The mass producing method of regenerated plant from the leaf segment of calanthe discolor | |
| CN104663456A (en) | Dendrobium officinale broken capsule and seed bottle-sowing tissue culture method | |
| CN105368742A (en) | Method for screening and separating PGPR containing ACC deaminase | |
| CN106305419B (en) | A kind of method of open culture sugar-cane tissue culture seedlings |
Legal Events
| Date | Code | Title | Description |
|---|---|---|---|
| PB01 | Publication | ||
| PB01 | Publication | ||
| SE01 | Entry into force of request for substantive examination | ||
| SE01 | Entry into force of request for substantive examination | ||
| RJ01 | Rejection of invention patent application after publication | ||
| RJ01 | Rejection of invention patent application after publication |
Application publication date: 20200211 |