CN110755319A - Hemp flower and leaf fermentation method, hemp flower and leaf fermentation extract and cosmetics - Google Patents
Hemp flower and leaf fermentation method, hemp flower and leaf fermentation extract and cosmetics Download PDFInfo
- Publication number
- CN110755319A CN110755319A CN201911010552.XA CN201911010552A CN110755319A CN 110755319 A CN110755319 A CN 110755319A CN 201911010552 A CN201911010552 A CN 201911010552A CN 110755319 A CN110755319 A CN 110755319A
- Authority
- CN
- China
- Prior art keywords
- leaf
- hemp flower
- hemp
- fermentation
- flower
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Pending
Links
Classifications
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K8/00—Cosmetics or similar toiletry preparations
- A61K8/18—Cosmetics or similar toiletry preparations characterised by the composition
- A61K8/96—Cosmetics or similar toiletry preparations characterised by the composition containing materials, or derivatives thereof of undetermined constitution
- A61K8/97—Cosmetics or similar toiletry preparations characterised by the composition containing materials, or derivatives thereof of undetermined constitution from algae, fungi, lichens or plants; from derivatives thereof
- A61K8/9783—Angiosperms [Magnoliophyta]
- A61K8/9789—Magnoliopsida [dicotyledons]
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61Q—SPECIFIC USE OF COSMETICS OR SIMILAR TOILETRY PREPARATIONS
- A61Q17/00—Barrier preparations; Preparations brought into direct contact with the skin for affording protection against external influences, e.g. sunlight, X-rays or other harmful rays, corrosive materials, bacteria or insect stings
- A61Q17/005—Antimicrobial preparations
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N1/00—Microorganisms, e.g. protozoa; Compositions thereof; Processes of propagating, maintaining or preserving microorganisms or compositions thereof; Processes of preparing or isolating a composition containing a microorganism; Culture media therefor
- C12N1/14—Fungi; Culture media therefor
- C12N1/16—Yeasts; Culture media therefor
- C12N1/18—Baker's yeast; Brewer's yeast
- C12N1/185—Saccharomyces isolates
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N1/00—Microorganisms, e.g. protozoa; Compositions thereof; Processes of propagating, maintaining or preserving microorganisms or compositions thereof; Processes of preparing or isolating a composition containing a microorganism; Culture media therefor
- C12N1/20—Bacteria; Culture media therefor
- C12N1/205—Bacterial isolates
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12P—FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE
- C12P1/00—Preparation of compounds or compositions, not provided for in groups C12P3/00 - C12P39/00, by using microorganisms or enzymes
- C12P1/02—Preparation of compounds or compositions, not provided for in groups C12P3/00 - C12P39/00, by using microorganisms or enzymes by using fungi
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12P—FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE
- C12P1/00—Preparation of compounds or compositions, not provided for in groups C12P3/00 - C12P39/00, by using microorganisms or enzymes
- C12P1/04—Preparation of compounds or compositions, not provided for in groups C12P3/00 - C12P39/00, by using microorganisms or enzymes by using bacteria
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12P—FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE
- C12P39/00—Processes involving microorganisms of different genera in the same process, simultaneously
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K2800/00—Properties of cosmetic compositions or active ingredients thereof or formulation aids used therein and process related aspects
- A61K2800/80—Process related aspects concerning the preparation of the cosmetic composition or the storage or application thereof
- A61K2800/85—Products or compounds obtained by fermentation, e.g. yoghurt, beer, wine
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12R—INDEXING SCHEME ASSOCIATED WITH SUBCLASSES C12C - C12Q, RELATING TO MICROORGANISMS
- C12R2001/00—Microorganisms ; Processes using microorganisms
- C12R2001/01—Bacteria or Actinomycetales ; using bacteria or Actinomycetales
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12R—INDEXING SCHEME ASSOCIATED WITH SUBCLASSES C12C - C12Q, RELATING TO MICROORGANISMS
- C12R2001/00—Microorganisms ; Processes using microorganisms
- C12R2001/01—Bacteria or Actinomycetales ; using bacteria or Actinomycetales
- C12R2001/225—Lactobacillus
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12R—INDEXING SCHEME ASSOCIATED WITH SUBCLASSES C12C - C12Q, RELATING TO MICROORGANISMS
- C12R2001/00—Microorganisms ; Processes using microorganisms
- C12R2001/645—Fungi ; Processes using fungi
- C12R2001/85—Saccharomyces
- C12R2001/865—Saccharomyces cerevisiae
Landscapes
- Life Sciences & Earth Sciences (AREA)
- Chemical & Material Sciences (AREA)
- Organic Chemistry (AREA)
- Engineering & Computer Science (AREA)
- Health & Medical Sciences (AREA)
- Wood Science & Technology (AREA)
- Zoology (AREA)
- Biotechnology (AREA)
- General Health & Medical Sciences (AREA)
- Genetics & Genomics (AREA)
- Bioinformatics & Cheminformatics (AREA)
- Mycology (AREA)
- Microbiology (AREA)
- General Engineering & Computer Science (AREA)
- Biochemistry (AREA)
- General Chemical & Material Sciences (AREA)
- Chemical Kinetics & Catalysis (AREA)
- Tropical Medicine & Parasitology (AREA)
- Animal Behavior & Ethology (AREA)
- Public Health (AREA)
- Veterinary Medicine (AREA)
- Botany (AREA)
- Biomedical Technology (AREA)
- Virology (AREA)
- Medicinal Chemistry (AREA)
- Dermatology (AREA)
- Epidemiology (AREA)
- Birds (AREA)
- Cosmetics (AREA)
Abstract
The invention relates to the technical field of fermentation, in particular to a hemp flower and leaf fermentation method, a hemp flower and leaf fermentation extract and cosmetics. The hemp flower and leaf fermentation method comprises the step of adding zymophyte liquid into hemp flower and leaf slurry for fermentation. The hemp flower and leaf fermentation extract prepared by the hemp flower and leaf fermentation method can be used for preparing cosmetics. The hemp flower and leaf fermentation method obtains more and smaller active molecular components through fermentation, so that the prepared fermentation extract has better antibacterial effect, and the cosmetics containing the fermentation extract have better antibacterial effect.
Description
Technical Field
The invention relates to the technical field of fermentation, in particular to a hemp flower and leaf fermentation method, a hemp flower and leaf fermentation extract and cosmetics.
Background
Industrial Cannabis is also known as hemp (hemp), is a 1-year-old herbal plant of Cannabis (Cannabinaceae) in Cannabis, and has a wide application.
The related art provides a method for preparing a hemp flower and leaf extract, which is difficult to sufficiently exert the antibacterial effect of an active substance.
Disclosure of Invention
The invention aims to provide a hemp flower and leaf fermentation method, a hemp flower and leaf fermentation extract and a cosmetic, which can obtain more and smaller active molecular components through fermentation so as to ensure that the prepared fermentation extract has better antibacterial effect and the cosmetic containing the fermentation extract has better antibacterial effect.
The invention is realized by the following steps:
in a first aspect, an embodiment of the present invention provides a hemp flower and leaf fermentation method, including: adding zymophyte liquid into hemp flower and leaf slurry for fermentation.
In an alternative embodiment, the fermentation broth comprises a liquid fermentation broth in log phase.
In an alternative embodiment, the method for preparing the zymogen liquid comprises the following steps: inoculating the strain to yeast extract peptone glucose culture medium, and culturing at 25-32 deg.C.
In an alternative embodiment, the yeast extract peptone glucose medium comprises, in parts by weight: 1.0-1.5% of yeast extract powder, 2.2-2.5% of peptone and 2.5-2.6% of glucose.
In an alternative embodiment, the fermentation broth comprises: at least one of yellow wine yeast zymocyte liquid, sake yeast zymocyte liquid, fruit wine yeast zymocyte liquid, vinegar brewing yeast zymocyte liquid, lactobacillus zymocyte liquid, bifidobacterium zymocyte liquid and lactococcus zymocyte liquid.
In an alternative embodiment, a method of preparing a hemp flower and leaf slurry comprises: mixing undried hemp flower and leaf with water at a weight ratio of 1:6-10, and pulping.
In an alternative embodiment, the hemp flower and leaf slurry has a pH of 6-8.
In an alternative embodiment, the method further comprises: after fermentation, centrifuging at the rotation speed of 11000-13000r/min and the centrifugal radius of 8-10 cm.
In a second aspect, embodiments of the present invention provide a cannabis flower and leaf fermentation extract prepared by the cannabis flower and leaf fermentation method of any one of the preceding embodiments.
In a third aspect, embodiments of the present invention provide a cosmetic comprising the fermented extract of cannabis flowers and leaves of the previous embodiments.
The hemp flower and leaf fermentation method provided by the embodiment of the invention has the beneficial effects that: according to the hemp flower and leaf fermentation method provided by the embodiment of the invention, the zymocyte liquid is added into the hemp flower and leaf slurry for fermentation, so that active substances in the hemp flower and leaf can be fully extracted, especially some small molecular components; thus, a fermented extract having a better antibacterial effect can be obtained by fermenting hemp flower and leaf pulp.
The hemp flower and leaf fermentation extract provided by the embodiment of the invention has the beneficial effects that: the hemp flower and leaf fermented extract provided by the embodiment of the invention is prepared by the fermentation method, and has better antibacterial effect.
The cosmetics of the embodiment of the invention have the beneficial effects that: the cosmetic provided by the embodiment of the invention comprises the hemp flower and leaf fermentation extract, and has a better antibacterial effect.
Detailed Description
In order to make the objects, technical solutions and advantages of the embodiments of the present invention clearer, the technical solutions in the embodiments of the present invention will be clearly and completely described below. The examples, in which specific conditions are not specified, were conducted under conventional conditions or conditions recommended by the manufacturer. The reagents or instruments used are not indicated by the manufacturer, and are all conventional products available commercially.
The hemp flower and leaf fermentation method, the hemp flower and leaf fermentation extract and the cosmetics of the present invention are further described in detail below.
The hemp flower and leaf fermentation method provided by the invention comprises the following steps: adding zymophyte liquid into hemp flower and leaf slurry for fermentation.
The active substances in the hemp flowers and leaves can be promoted to be fully released, particularly some small molecular components, by adding zymocyte liquid into the hemp flower and leaf serous fluid for fermentation; thus, a fermented extract having a better antibacterial effect can be obtained by fermenting hemp flower and leaf pulp.
The preparation method of the hemp flower and leaf pulp comprises the following steps: mixing undried hemp flower and leaf with water at a weight ratio of 1:6-10, and pulping. After pulping, autoclaving at 110-125 deg.C for 15-20 min; and then adjusting the pH of the serous fluid to 6-8, thus completing the preparation of the hemp flower and leaf serous fluid.
The undried hemp flowers and leaves may be: fresh hemp flowers and leaves; thus, the fresh hemp flower and leaf is used for preparing the slurry, and the loss of active substances such as terpenes, cannabidiol and the like in the hemp flower and leaf can be reduced.
When the hemp flower and leaf pulp is sterilized, the hemp flower and leaf pulp is quickly sterilized at high temperature in a short time, and the loss of active substances such as terpenes, cannabidiol and the like can be further reduced.
The pH value of the hemp flower and leaf serous fluid is adjusted to 6-8, which is beneficial to the smooth development of the growth and fermentation process of the fungus subsequently added into the hemp flower and leaf serous fluid.
The preparation method of the zymocyte liquid comprises the following steps: inoculating the strain in liquid yeast extract peptone glucose culture medium, and culturing at 25-32 deg.C.
Further, the cultivation of the fermentation broth may be carried out in a shaker.
When the liquid fermentation according to the fermentation method reaches the logarithmic phase, the liquid zymogen liquid in the logarithmic phase can be added into the hemp flower and leaf slurry to be used as the zymogen liquid. The fermentation of hemp flower and leaf slurry is carried out by adopting the liquid zymogen liquid in the logarithmic phase, so that the thalli can be rapidly proliferated in the hemp flower and leaf slurry on one hand, and the fermentation process can be rapidly developed on the other hand.
It should be noted that the strain in the liquid fermentation can be used as a fermentation broth to be inoculated into the hemp flower-leaf slurry as long as the strain is in the logarithmic phase, and the specific concentration of the strain in the broth is not limited, i.e., various concentrations are possible.
The yeast extract peptone glucose culture medium comprises the following components in parts by weight: 1.0-1.5% of yeast extract powder, 2.2-2.5% of peptone and 2.5-2.6% of glucose.
The yeast extract peptone glucose medium may be sterilized by a conventional method after preparation.
The zymogen liquid used in the invention comprises: at least one of yellow wine yeast zymocyte liquid, sake yeast zymocyte liquid, fruit wine yeast zymocyte liquid, vinegar brewing yeast zymocyte liquid, lactobacillus zymocyte liquid, bifidobacterium zymocyte liquid and lactococcus zymocyte liquid; namely, the strains used in the present invention include: at least one of yellow wine yeast, sake yeast, fruit wine yeast, vinegar brewing yeast, lactobacillus, bifidobacterium and lactococcus.
The method of inoculating the strain in the liquid yeast extract peptone glucose medium is similar to the related art, and for example, inoculation with an inoculating loop is sufficient.
In the hemp flower and leaf fermentation method, 3 times of prepared zymophyte liquid can be added into the hemp flower and leaf serous fluid.
During fermentation, the temperature can be controlled to be 25-36 ℃ on a shaking table, and the fermentation time can be about 20-65 h.
After the fermentation is finished, centrifuging under the conditions that the centrifugal radius is 8-10cm and the rotating speed is 11000-13000r/min, and collecting supernatant, namely the hemp flower and leaf fermentation extract.
The hemp flower and leaf fermentation extract prepared by the hemp flower and leaf fermentation method provided by the invention can be directly used as a finished product of a mask or an essence or toner; the prepared hemp flower and leaf fermented extract can be directly used as a cosmetic, so that the production cost can be saved to a certain extent, the product quality is improved, the production steps are simplified to the maximum extent, the mass production and the industrial production can be realized, the stability of the product quality can be fully ensured, and the natural substance used as the cosmetic is not easy to cause burden on the skin of a user.
When the hemp flower and leaf fermented extract is directly used as a cosmetic, the hemp flower and leaf fermented extract can be filled in a 10 ten thousand grade purification workshop and then is subjected to sterilization treatment, wherein the sterilization conditions are as follows: 110 ℃ and 125 ℃, and high-pressure and high-temperature sterilization is carried out for 20-25 min.
The cosmetic provided by the present invention is not limited to the above facial mask, essence or toner, but can also be prepared into gel, paste, etc. by using hemp flower and leaf fermented extract, and is not specifically limited herein. The cosmetics containing the hemp flower and leaf fermentation extract provided by the invention are easy to absorb and have good effect.
The hemp flower and leaf fermentation method, the hemp flower and leaf fermentation extract and the cosmetics of the present invention are further described in detail with reference to examples below.
Example 1
Preparing a liquid yeast extract peptone glucose culture medium, which comprises the following components in percentage by weight: 1.0% of yeast extract powder, 2.2% of peptone and 2.5% of glucose.
Inoculating yellow wine yeast to the sterilized liquid yeast extract peptone glucose culture medium, carrying out shake culture in a shaker at 25 ℃, and taking the fermentation liquor of logarithmic phase as the fermentation liquor of subsequent fermentation.
Preparing hemp flower and leaf pulp from fresh hemp flower and leaf and water at a weight ratio of 1:8, sterilizing at 110 deg.C for 20min, and adjusting pH to 6.
Adding zymocyte liquid with the weight ratio of 3 times of the hemp flower and leaf slurry, and fermenting in a shaking table at the temperature of 25 ℃ for 65 hours; centrifuging the fermented broth for 21min at a rotation speed of 11000r/min and a centrifugation radius of 10cm, and collecting supernatant to obtain the hemp leaf fermented extract.
Filling the hemp flower and leaf fermentation extract in a 10 ten thousand grade purification workshop, and then performing sterilization treatment, wherein the sterilization conditions are as follows: sterilizing at 110 deg.C for 25min under high pressure; thus preparing a cosmetic.
Example 2
Preparing a liquid yeast extract peptone glucose culture medium, which comprises the following components in percentage by weight: 1.5 percent of yeast extract powder, 2.5 percent of peptone and 2.6 percent of glucose.
Inoculating the sake yeast and the fruit wine yeast together to a sterilized liquid yeast extract peptone glucose culture medium, carrying out shake culture in a shaking table at 32 ℃, and taking the fermentation liquor of logarithmic phase as the fermentation liquor of subsequent fermentation.
Preparing hemp flower and leaf pulp from fresh hemp flower and leaf and water at a weight ratio of 1:6, sterilizing at 125 deg.C for 15min, and adjusting pH to 8.
Adding zymophyte liquid with the weight ratio of 3 times of the hemp flower and leaf serous fluid, and fermenting for 20 hours in a shaking table at the temperature of 36 ℃; centrifuging the fermented broth at 13000r/min and 8cm radius for 19min, and collecting supernatant to obtain hemp leaf fermented extract.
Filling the hemp flower and leaf fermentation extract in a 10 ten thousand grade purification workshop, and then performing sterilization treatment, wherein the sterilization conditions are as follows: sterilizing at 125 deg.C for 20min under high pressure and high temperature; thus preparing a cosmetic.
Example 3
Preparing a liquid yeast extract peptone glucose culture medium, which comprises the following components in percentage by weight: 1.5 percent of yeast extract powder, 2.4 percent of peptone and 2.5 percent of glucose.
Inoculating vinegar brewing yeast and lactobacillus into sterilized liquid yeast extract peptone glucose culture medium, performing shake culture in a shaking table at 30 ℃, and taking logarithmic phase fermentation liquor as fermentation liquor of subsequent fermentation.
Preparing hemp flower and leaf slurry from fresh hemp flower and leaf and water at a weight ratio of 1:10, sterilizing at 120 deg.C for 18min, and adjusting pH to 7.
Adding 3 times of zymocyte liquid by weight of hemp flower and leaf slurry, and fermenting in a shaking table at 32 ℃ for 40 h; centrifuging the fermented broth at 12000r/min with centrifugation radius of 9cm for 20min, and collecting supernatant to obtain hemp leaf fermented extract.
Filling the hemp flower and leaf fermentation extract in a 10 ten thousand grade purification workshop, and then performing sterilization treatment, wherein the sterilization conditions are as follows: sterilizing at 120 deg.C for 22min under high pressure and high temperature; thus preparing a cosmetic.
Example 4
Preparing a liquid yeast extract peptone glucose culture medium, which comprises the following components in percentage by weight: 1.2% of yeast extract powder, 2.2% of peptone and 2.6% of glucose.
Inoculating Bifidobacterium and lactococcus together in sterilized liquid yeast to extract peptone glucose culture medium, performing shake culture in a shaking table at 27 deg.C, and taking logarithmic phase fermentation broth as fermentation broth for subsequent fermentation.
Preparing hemp flower and leaf slurry from fresh hemp flower and leaf and water at a weight ratio of 1:9, sterilizing at 110 deg.C for 18min, and adjusting pH to 6.
Adding zymophyte liquid with the weight ratio of 3 times of the hemp flower and leaf slurry, and fermenting for 35 hours in a shaking table at the temperature of 32 ℃; centrifuging the fermented broth at 12000r/min with a centrifugation radius of 10cm for 20min, and collecting supernatant to obtain hemp leaf fermented extract.
Filling the hemp flower and leaf fermentation extract in a 10 ten thousand grade purification workshop, and then performing sterilization treatment, wherein the sterilization conditions are as follows: sterilizing at 110 deg.C for 25min under high pressure; thus preparing a cosmetic.
Example 5
Preparing a liquid yeast extract peptone glucose culture medium, which comprises the following components in percentage by weight: 1.0% of yeast extract powder, 2.2% of peptone and 2.5% of glucose.
Inoculating yellow wine yeast to the sterilized liquid yeast extract peptone glucose culture medium, carrying out shake culture in a shaker at 25 ℃, and taking the fermentation liquor of logarithmic phase as the fermentation liquor of subsequent fermentation.
Preparing hemp flower and leaf slurry from dried hemp flower and leaf and water at a weight ratio of 1:20, sterilizing at 110 deg.C for 20min, and adjusting pH to 6.
Adding zymocyte liquid with the weight ratio of 3 times of the hemp flower and leaf slurry, and fermenting in a shaking table at the temperature of 25 ℃ for 65 hours; centrifuging the fermented broth for 21min at a rotation speed of 11000r/min and a centrifugation radius of 10cm, and collecting supernatant to obtain the hemp leaf fermented extract.
Filling the hemp flower and leaf fermentation extract in a 10 ten thousand grade purification workshop, and then performing sterilization treatment, wherein the sterilization conditions are as follows: sterilizing at 110 deg.C for 25min under high pressure; thus preparing a cosmetic.
Example 6
Preparing a liquid yeast extract peptone glucose culture medium, which comprises the following components in percentage by weight: 1.0% of yeast extract powder, 2.2% of peptone and 2.5% of glucose.
Inoculating yellow wine yeast to the sterilized liquid yeast extract peptone glucose culture medium, and performing shake culture in a shaker at 25 ℃, wherein fermentation liquor which has not reached the logarithmic phase is used as fermentation liquor for subsequent fermentation.
Preparing hemp flower and leaf pulp from fresh hemp flower and leaf and water at a weight ratio of 1:8, sterilizing at 110 deg.C for 20min, and adjusting pH to 6.
Adding zymocyte liquid with the weight ratio of 3 times of the hemp flower and leaf slurry, and fermenting in a shaking table at the temperature of 25 ℃ for 65 hours; centrifuging the fermented broth for 21min at a rotation speed of 11000r/min and a centrifugation radius of 10cm, and collecting supernatant to obtain the hemp leaf fermented extract.
Filling the hemp flower and leaf fermentation extract in a 10 ten thousand grade purification workshop, and then performing sterilization treatment, wherein the sterilization conditions are as follows: sterilizing at 110 deg.C for 25min under high pressure; thus preparing a cosmetic.
Firstly, detecting antibacterial performance:
1) the antibacterial experiment of the hemp flower and leaf fermented extract provided in example 1 was carried out by the following experimental units: university of Guangdong pharmacy.
Etiology analysis considers that the severity of acne is related to the proliferation of propionibacterium acnes, so that the current evaluation on the activity of anti-acne medicines mainly considers the bacteriostatic action on the propionibacterium acnes; the strain used in this test was Propionibacterium acnes (Propionibacterium acnes), supplied by the institute for microorganisms in Guangdong province. The reagents involved in the test also included: triclosan DP300 (michelin).
The main equipment is as follows: KQ3200DA ultrasonic cleaner (kunshan supermarket instrument ltd); LDZX-50KBS autoclave (Shanghai Shenan medical instruments factory); an electronic scale of one ten thousandth of SQP (sydow scientific instruments (beijing) ltd.).
The main method for testing comprises the following steps:
the test substance (the fermented extract of hemp flowers and leaves provided in example 1) was dissolved in sterilized normal saline to a certain concentration (1 time), and diluted for use. Respectively inoculating 1mL of fresh bacterial liquid into nutrient broth culture media, adding the test substance solutions with different concentrations, placing the test substance solutions in a common incubator at 37 ℃ for culturing for 72h, and observing the result. A blank control group (physiological saline) and a positive control group DP300 were also provided.
According to the content setting of inhibiting the growth of microorganisms in 2015 edition of pharmacopoeia of the people's republic of China, the bacteriostatic effect is divided into 4 grades according to the size of a bacteriostatic zone. See table 1.
TABLE 1 evaluation criteria for the efficacy of inhibition of Propionibacterium acnes
And (3) judging the result of the minimum bacteriostatic concentration: if the culture medium is turbid, the bacteria grow, and the liquid medicine has no bacteriostatic action; the transparent culture medium shows aseptic growth, and the medicinal liquid has antibacterial effect. The Minimum Inhibitory Concentration (MIC) is the concentration of the liquid medicine for inhibiting the growth of bacteria at the maximum dilution factor.
The results of the test are shown in Table 2.
TABLE 2 determination of MIC of fermented extract of Cannabis sativa flowers and leaves for Propionibacterium acnes
According to the results in table 2, the colony growth area of the plate added with the normal saline is 100%, and the colony growth area of the positive control group added with 0.1% DP300 is 0.0%, which indicates that the solvent normal saline has no interference to the bacteriostatic test result of the hemp flower and leaf fermented extract, and the experimental result is effective. As can be seen from the data in Table 2, the fermented extract of Cannabis sativa flower and leaf had inhibitory effects on the colony growth area of Propionibacterium acnes at concentrations of 6.25%, 12.5%, 25% and 50%. Therefore, the marijuana flower and leaf fermentation extract has obvious inhibition effect on propionibacterium acnes.
2) The antibacterial activity of the fermented extract of cannabis sativa obtained in examples 1-6 was tested in a manner similar to that of 1), and the concentration of the fermented extract of cannabis sativa obtained in examples 1-6 was diluted to 25% and 50% at the same time, and the test was performed. The results are shown in Table 3.
TABLE 3 determination of MIC of various groups of fermented extracts of Cannabis sativa flowers and leaves for Propionibacterium acnes
According to the results in table 3, the hemp flower and leaf fermented extract prepared by the preparation method provided by the invention has good antibacterial performance.
Among them, the test results of examples 1 to 4 are superior to those of examples 5 and 6, and thus it can be seen that the antibacterial effect of the hemp flower and leaf fermented extract prepared by fermenting fresh hemp flower and leaf is superior to that of the fermented extract prepared by drying hemp flower and leaf; the antibacterial effect of the fermented extract prepared by adding the bacterial liquid in the logarithmic phase as the zymocyte liquid into the hemp flower and leaf slurry for fermentation is better.
In summary, according to the hemp flower and leaf fermentation method provided by the embodiment of the invention, the zymogen liquid is added into the hemp flower and leaf slurry for fermentation, so that active substances in the hemp flower and leaf can be fully extracted, especially some small molecular components; thus, a fermented extract having a better antibacterial effect can be obtained by fermenting hemp flower and leaf pulp.
The hemp flower and leaf fermented extract provided by the embodiment of the invention is prepared by the fermentation method, and has better antibacterial effect.
The cosmetic provided by the embodiment of the invention comprises the hemp flower and leaf fermentation extract, and has a better antibacterial effect.
The above description is only a preferred embodiment of the present invention and is not intended to limit the present invention, and various modifications and changes may be made by those skilled in the art. Any modification, equivalent replacement, or improvement made within the spirit and principle of the present invention should be included in the protection scope of the present invention.
Claims (10)
1. A hemp flower and leaf fermentation method is characterized by comprising the following steps: adding zymophyte liquid into hemp flower and leaf slurry for fermentation.
2. The hemp flower and leaf fermentation method of claim 1, wherein the zymogen liquid comprises a liquid zymogen liquid in log phase.
3. The hemp flower and leaf fermentation method according to claim 1, wherein the preparation method of the zymocyte liquid comprises the following steps: inoculating the strain to yeast extract peptone glucose culture medium, and culturing at 25-32 deg.C.
4. The hemp flower and leaf fermentation method according to claim 3, wherein the yeast extract peptone glucose medium comprises, in parts by weight: 1.0-1.5% of yeast extract powder, 2.2-2.5% of peptone and 2.5-2.6% of glucose.
5. The hemp flower and leaf fermentation method of claim 1, wherein the zymogen liquid comprises: at least one of yellow wine yeast zymocyte liquid, sake yeast zymocyte liquid, fruit wine yeast zymocyte liquid, vinegar brewing yeast zymocyte liquid, lactobacillus zymocyte liquid, bifidobacterium zymocyte liquid and lactococcus zymocyte liquid.
6. The hemp flower and leaf fermentation method of claim 1, wherein the preparation method of the hemp flower and leaf slurry comprises: mixing undried hemp flower and leaf with water at a weight ratio of 1:6-10, and pulping.
7. The hemp flower and leaf fermentation process of claim 1, wherein the hemp flower and leaf slurry has a pH of 6-8.
8. The hemp flower and leaf fermentation method of claim 1, further comprising: after fermentation, centrifuging at the rotation speed of 11000-13000r/min and the centrifugal radius of 8-10 cm.
9. A fermented extract of hemp flowers and leaves, which is prepared by the fermentation method of hemp flowers and leaves according to any one of claims 1 to 8.
10. A cosmetic comprising the fermented extract of hemp flower and leaf according to claim 9.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201911010552.XA CN110755319A (en) | 2019-10-23 | 2019-10-23 | Hemp flower and leaf fermentation method, hemp flower and leaf fermentation extract and cosmetics |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201911010552.XA CN110755319A (en) | 2019-10-23 | 2019-10-23 | Hemp flower and leaf fermentation method, hemp flower and leaf fermentation extract and cosmetics |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| CN110755319A true CN110755319A (en) | 2020-02-07 |
Family
ID=69332948
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN201911010552.XA Pending CN110755319A (en) | 2019-10-23 | 2019-10-23 | Hemp flower and leaf fermentation method, hemp flower and leaf fermentation extract and cosmetics |
Country Status (1)
| Country | Link |
|---|---|
| CN (1) | CN110755319A (en) |
Cited By (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN111437224A (en) * | 2020-04-20 | 2020-07-24 | 中国农业科学院麻类研究所 | Method for extracting antioxidant component from cannabis sativa leaves by using microorganisms and application of antioxidant component |
| WO2023147496A1 (en) * | 2022-01-28 | 2023-08-03 | San Martin Cbd Llc | Natural extraction of cannabis-based phytocompounds from cannabis plants through yeast fermentation |
Citations (4)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| KR20160017227A (en) * | 2014-08-01 | 2016-02-16 | 장복현 | Functional Fermented soft drinks. Fermented alcohol. Raw materials for cosmetics and skin treatment. Fermentation and manufacture method |
| KR20170085990A (en) * | 2016-01-15 | 2017-07-25 | 김유근 | Method of increase the bioactive effectiveness of sprout ginseng |
| CN109806203A (en) * | 2019-04-03 | 2019-05-28 | 栾云鹏 | A kind of shampoo of the extract of floral leaf containing industrial hemp |
| CN110041172A (en) * | 2019-06-05 | 2019-07-23 | 云南绿新生物药业有限公司 | A kind of technique improving cannabidiol recovery rate using microbiological treatment hemp floral leaf |
-
2019
- 2019-10-23 CN CN201911010552.XA patent/CN110755319A/en active Pending
Patent Citations (4)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| KR20160017227A (en) * | 2014-08-01 | 2016-02-16 | 장복현 | Functional Fermented soft drinks. Fermented alcohol. Raw materials for cosmetics and skin treatment. Fermentation and manufacture method |
| KR20170085990A (en) * | 2016-01-15 | 2017-07-25 | 김유근 | Method of increase the bioactive effectiveness of sprout ginseng |
| CN109806203A (en) * | 2019-04-03 | 2019-05-28 | 栾云鹏 | A kind of shampoo of the extract of floral leaf containing industrial hemp |
| CN110041172A (en) * | 2019-06-05 | 2019-07-23 | 云南绿新生物药业有限公司 | A kind of technique improving cannabidiol recovery rate using microbiological treatment hemp floral leaf |
Non-Patent Citations (9)
| Title |
|---|
| 国家药品监督管理局: "国家药监局关于更新化妆品禁用原料目录的公告（2021年第74号）", 《HTTPS://WWW.NMPA.GOV.CN/XXGK/GGTG/QTGGTG/20210528174051160.HTML》 * |
| 孔剑梅等: "工业大麻花叶提取大麻二酚工艺技术综述", 《云南化工》 * |
| 孙进昌等: "麻类副产物的综合开发与利用价值", 《农产品加工(学刊)》 * |
| 张文治: "《新编食品微生物学》", 31 December 1995, 中国轻工业出版社 * |
| 张正海等: "响应面法优化工业大麻叶抑制金黄色葡萄球菌物质超声提取工艺", 《中国麻业科学》 * |
| 桑亚新等: "《普通高等教育"十三五"规划教材 食品微生物学》", 31 January 2017, 中国轻工业出版社 * |
| 白毓谦等: "《微生物实验技术》", 31 May 1987, 山东大学出版社 * |
| 霍乃蕊等: "《微生物生物学》", 30 April 2018, 中国农业大学出版社 * |
| 黄芳一等: "《发酵工程》", 31 May 2019, 华中师范大学出版社 * |
Cited By (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN111437224A (en) * | 2020-04-20 | 2020-07-24 | 中国农业科学院麻类研究所 | Method for extracting antioxidant component from cannabis sativa leaves by using microorganisms and application of antioxidant component |
| CN111437224B (en) * | 2020-04-20 | 2022-08-09 | 中国农业科学院麻类研究所 | Method for extracting antioxidant component from cannabis sativa leaves by using microorganisms and application of antioxidant component |
| WO2023147496A1 (en) * | 2022-01-28 | 2023-08-03 | San Martin Cbd Llc | Natural extraction of cannabis-based phytocompounds from cannabis plants through yeast fermentation |
Similar Documents
| Publication | Publication Date | Title |
|---|---|---|
| CN110250210B (en) | Optimal DSE strain for promoting corn seed soaking and rooting | |
| Zhang et al. | Physical adsorption of patulin by Saccharomyces cerevisiae during fermentation | |
| CN109893487B (en) | Rice rose fermented raw pulp and preparation method and application thereof | |
| CN113337545B (en) | Schizophyllum commune fermentation product, preparation method thereof, skin care product and schizophyllum commune culture medium | |
| CN106497806A (en) | A kind of coronoid process dissipate capsule bacterium strain and its application | |
| CN110200018B (en) | Optimal DSE inoculation amount for promoting plant rooting | |
| CN113143812B (en) | Preparation method of kava pepper fermentation product, kava pepper fermentation product and application of kava pepper fermentation product in cosmetics | |
| CN110755319A (en) | Hemp flower and leaf fermentation method, hemp flower and leaf fermentation extract and cosmetics | |
| CN109207548B (en) | Peanut coat oligomeric proanthocyanidin, and preparation method and application thereof | |
| WO2021093299A1 (en) | Astragalus-paecilomyces cicadae fermented fungal substance, preparation method and application thereof | |
| KR101793905B1 (en) | Biocellulose using watermelon or sugarcane and manufacturing methods thereof | |
| CN116376731B (en) | Application of Wilkham yeast in preparing Prinsepia utilis extract | |
| CN115671024B (en) | Cherry flower glabridin composite fermentation liquid, product containing same, preparation and application thereof | |
| CN109749939B (en) | Microbial inoculum containing salvia miltiorrhiza endophytic fungi and application thereof | |
| CN111587893A (en) | Aureobasidium pullulans fermentation product and preparation method thereof, microbial preparation for reducing fungal diseases and method for reducing fungal diseases of agricultural products | |
| CN112402334A (en) | Application of highland barley fermentation extract | |
| CN104762353B (en) | One Bacillus species bacteriocin extract and its preparation method and application | |
| CN104726516B (en) | A kind of method that Nano bacteria cellulose is prepared using raffinose | |
| CN109810918B (en) | Bacillus atrophaeus with effect of preventing wolfberry leaf blight, biological agent and application of biological agent | |
| CN106520576B (en) | A kind of sclerotium mould and its application | |
| CN113632922A (en) | Lysate, preparation method and application | |
| CN116855392B (en) | Houttuynia cordata fermentation broth and preparation method and application thereof | |
| CN104711304A (en) | Method for producing germ nano-crystalline cellulose by utilizing tea fungus and raffinose | |
| CN111297777B (en) | Preparation method of wheat primary pulp, product and application thereof | |
| CN115918833B (en) | Method for removing astringency of roxburgh rose juice with high efficiency and reduced loss of active ingredients |
Legal Events
| Date | Code | Title | Description |
|---|---|---|---|
| PB01 | Publication | ||
| PB01 | Publication | ||
| SE01 | Entry into force of request for substantive examination | ||
| SE01 | Entry into force of request for substantive examination | ||
| RJ01 | Rejection of invention patent application after publication |
Application publication date: 20200207 |
|
| RJ01 | Rejection of invention patent application after publication |