CN110754665A - Method for extracting potassium element from beef tripe - Google Patents
Method for extracting potassium element from beef tripe Download PDFInfo
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- CN110754665A CN110754665A CN201911045675.7A CN201911045675A CN110754665A CN 110754665 A CN110754665 A CN 110754665A CN 201911045675 A CN201911045675 A CN 201911045675A CN 110754665 A CN110754665 A CN 110754665A
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- beef tripe
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- 235000015278 beef Nutrition 0.000 title claims abstract description 71
- 239000011591 potassium Substances 0.000 title claims abstract description 47
- 229910052700 potassium Inorganic materials 0.000 title claims abstract description 47
- ZLMJMSJWJFRBEC-UHFFFAOYSA-N Potassium Chemical compound [K] ZLMJMSJWJFRBEC-UHFFFAOYSA-N 0.000 title claims abstract description 46
- 238000000034 method Methods 0.000 title claims abstract description 26
- 230000005684 electric field Effects 0.000 claims abstract description 20
- 238000001035 drying Methods 0.000 claims abstract description 19
- 238000000926 separation method Methods 0.000 claims abstract description 13
- 238000000498 ball milling Methods 0.000 claims abstract description 7
- 238000005238 degreasing Methods 0.000 claims abstract description 7
- 230000005415 magnetization Effects 0.000 claims abstract description 7
- 230000005291 magnetic effect Effects 0.000 claims description 38
- 241000186660 Lactobacillus Species 0.000 claims description 31
- 229940039696 lactobacillus Drugs 0.000 claims description 31
- 239000000203 mixture Substances 0.000 claims description 29
- 239000000843 powder Substances 0.000 claims description 28
- 244000063299 Bacillus subtilis Species 0.000 claims description 25
- 235000014469 Bacillus subtilis Nutrition 0.000 claims description 25
- 238000002156 mixing Methods 0.000 claims description 25
- MUBZPKHOEPUJKR-UHFFFAOYSA-N Oxalic acid Chemical compound OC(=O)C(O)=O MUBZPKHOEPUJKR-UHFFFAOYSA-N 0.000 claims description 24
- 239000000284 extract Substances 0.000 claims description 24
- 238000003756 stirring Methods 0.000 claims description 24
- QTBSBXVTEAMEQO-UHFFFAOYSA-N Acetic acid Chemical compound CC(O)=O QTBSBXVTEAMEQO-UHFFFAOYSA-N 0.000 claims description 21
- KRKNYBCHXYNGOX-UHFFFAOYSA-N citric acid Chemical compound OC(=O)CC(O)(C(O)=O)CC(O)=O KRKNYBCHXYNGOX-UHFFFAOYSA-N 0.000 claims description 21
- 239000000725 suspension Substances 0.000 claims description 18
- 150000007524 organic acids Chemical class 0.000 claims description 16
- 235000017060 Arachis glabrata Nutrition 0.000 claims description 14
- 244000105624 Arachis hypogaea Species 0.000 claims description 14
- 235000010777 Arachis hypogaea Nutrition 0.000 claims description 14
- 235000018262 Arachis monticola Nutrition 0.000 claims description 14
- 244000060011 Cocos nucifera Species 0.000 claims description 14
- 235000013162 Cocos nucifera Nutrition 0.000 claims description 14
- 230000003213 activating effect Effects 0.000 claims description 14
- QHGJSLXSVXVKHZ-UHFFFAOYSA-N dilithium;dioxido(dioxo)manganese Chemical compound [Li+].[Li+].[O-][Mn]([O-])(=O)=O QHGJSLXSVXVKHZ-UHFFFAOYSA-N 0.000 claims description 14
- 238000010438 heat treatment Methods 0.000 claims description 14
- 235000020232 peanut Nutrition 0.000 claims description 14
- 238000001914 filtration Methods 0.000 claims description 13
- 238000002791 soaking Methods 0.000 claims description 13
- XLYOFNOQVPJJNP-UHFFFAOYSA-N water Substances O XLYOFNOQVPJJNP-UHFFFAOYSA-N 0.000 claims description 13
- 239000001963 growth medium Substances 0.000 claims description 12
- 238000004321 preservation Methods 0.000 claims description 12
- 239000006228 supernatant Substances 0.000 claims description 12
- UXVMQQNJUSDDNG-UHFFFAOYSA-L Calcium chloride Chemical compound [Cl-].[Cl-].[Ca+2] UXVMQQNJUSDDNG-UHFFFAOYSA-L 0.000 claims description 11
- 239000001110 calcium chloride Substances 0.000 claims description 11
- 229910001628 calcium chloride Inorganic materials 0.000 claims description 11
- 239000007788 liquid Substances 0.000 claims description 10
- 239000000126 substance Substances 0.000 claims description 9
- 238000009210 therapy by ultrasound Methods 0.000 claims description 9
- XEEYBQQBJWHFJM-UHFFFAOYSA-N Iron Chemical compound [Fe] XEEYBQQBJWHFJM-UHFFFAOYSA-N 0.000 claims description 8
- 230000004913 activation Effects 0.000 claims description 8
- 239000000706 filtrate Substances 0.000 claims description 8
- 235000006408 oxalic acid Nutrition 0.000 claims description 8
- WQZGKKKJIJFFOK-GASJEMHNSA-N Glucose Natural products OC[C@H]1OC(O)[C@H](O)[C@@H](O)[C@@H]1O WQZGKKKJIJFFOK-GASJEMHNSA-N 0.000 claims description 7
- 239000008103 glucose Substances 0.000 claims description 7
- 229920001817 Agar Polymers 0.000 claims description 6
- 239000001888 Peptone Substances 0.000 claims description 6
- 108010080698 Peptones Proteins 0.000 claims description 6
- VMHLLURERBWHNL-UHFFFAOYSA-M Sodium acetate Chemical compound [Na+].CC([O-])=O VMHLLURERBWHNL-UHFFFAOYSA-M 0.000 claims description 6
- 239000008272 agar Substances 0.000 claims description 6
- 238000003763 carbonization Methods 0.000 claims description 6
- 238000000855 fermentation Methods 0.000 claims description 6
- 230000004151 fermentation Effects 0.000 claims description 6
- 238000007710 freezing Methods 0.000 claims description 6
- 230000008014 freezing Effects 0.000 claims description 6
- 238000000227 grinding Methods 0.000 claims description 6
- 229910000402 monopotassium phosphate Inorganic materials 0.000 claims description 6
- 235000019796 monopotassium phosphate Nutrition 0.000 claims description 6
- 235000019319 peptone Nutrition 0.000 claims description 6
- PJNZPQUBCPKICU-UHFFFAOYSA-N phosphoric acid;potassium Chemical compound [K].OP(O)(O)=O PJNZPQUBCPKICU-UHFFFAOYSA-N 0.000 claims description 6
- 238000012545 processing Methods 0.000 claims description 6
- 239000000047 product Substances 0.000 claims description 6
- 230000001681 protective effect Effects 0.000 claims description 6
- 239000001632 sodium acetate Substances 0.000 claims description 6
- 235000017281 sodium acetate Nutrition 0.000 claims description 6
- YWYZEGXAUVWDED-UHFFFAOYSA-N triammonium citrate Chemical compound [NH4+].[NH4+].[NH4+].[O-]C(=O)CC(O)(CC([O-])=O)C([O-])=O YWYZEGXAUVWDED-UHFFFAOYSA-N 0.000 claims description 6
- 239000001393 triammonium citrate Substances 0.000 claims description 6
- 235000011046 triammonium citrate Nutrition 0.000 claims description 6
- CSNNHWWHGAXBCP-UHFFFAOYSA-L Magnesium sulfate Chemical compound [Mg+2].[O-][S+2]([O-])([O-])[O-] CSNNHWWHGAXBCP-UHFFFAOYSA-L 0.000 claims description 4
- 239000002002 slurry Substances 0.000 claims description 3
- 239000003610 charcoal Substances 0.000 claims description 2
- 229910052943 magnesium sulfate Inorganic materials 0.000 claims description 2
- 235000019341 magnesium sulphate Nutrition 0.000 claims description 2
- 239000000463 material Substances 0.000 claims description 2
- 230000000694 effects Effects 0.000 abstract description 8
- 238000004090 dissolution Methods 0.000 abstract description 5
- 238000000605 extraction Methods 0.000 abstract description 5
- 108090000623 proteins and genes Proteins 0.000 abstract description 5
- 102000004169 proteins and genes Human genes 0.000 abstract description 5
- 238000010521 absorption reaction Methods 0.000 abstract description 2
- 229910001414 potassium ion Inorganic materials 0.000 abstract description 2
- 239000000243 solution Substances 0.000 description 49
- 229960003975 potassium Drugs 0.000 description 33
- 241000283690 Bos taurus Species 0.000 description 13
- 238000002360 preparation method Methods 0.000 description 10
- 230000000052 comparative effect Effects 0.000 description 7
- 229920000858 Cyclodextrin Polymers 0.000 description 6
- HFHDHCJBZVLPGP-UHFFFAOYSA-N schardinger α-dextrin Chemical compound O1C(C(C2O)O)C(CO)OC2OC(C(C2O)O)C(CO)OC2OC(C(C2O)O)C(CO)OC2OC(C(O)C2O)C(CO)OC2OC(C(C2O)O)C(CO)OC2OC2C(O)C(O)C1OC2CO HFHDHCJBZVLPGP-UHFFFAOYSA-N 0.000 description 6
- 210000002787 omasum Anatomy 0.000 description 5
- 210000003165 abomasum Anatomy 0.000 description 4
- 229940041514 candida albicans extract Drugs 0.000 description 4
- 210000004767 rumen Anatomy 0.000 description 4
- 238000001694 spray drying Methods 0.000 description 4
- 239000012138 yeast extract Substances 0.000 description 4
- 102000008186 Collagen Human genes 0.000 description 3
- 108010035532 Collagen Proteins 0.000 description 3
- 229920001436 collagen Polymers 0.000 description 3
- 230000029087 digestion Effects 0.000 description 3
- XUFQPHANEAPEMJ-UHFFFAOYSA-N famotidine Chemical compound NC(N)=NC1=NC(CSCCC(N)=NS(N)(=O)=O)=CS1 XUFQPHANEAPEMJ-UHFFFAOYSA-N 0.000 description 3
- 210000003660 reticulum Anatomy 0.000 description 3
- 210000002784 stomach Anatomy 0.000 description 3
- KRHYYFGTRYWZRS-UHFFFAOYSA-N Fluorane Chemical compound F KRHYYFGTRYWZRS-UHFFFAOYSA-N 0.000 description 2
- 208000019025 Hypokalemia Diseases 0.000 description 2
- 239000002253 acid Substances 0.000 description 2
- 238000006243 chemical reaction Methods 0.000 description 2
- HVYWMOMLDIMFJA-DPAQBDIFSA-N cholesterol Chemical compound C1C=C2C[C@@H](O)CC[C@]2(C)[C@@H]2[C@@H]1[C@@H]1CC[C@H]([C@H](C)CCCC(C)C)[C@@]1(C)CC2 HVYWMOMLDIMFJA-DPAQBDIFSA-N 0.000 description 2
- 238000005259 measurement Methods 0.000 description 2
- VLTRZXGMWDSKGL-UHFFFAOYSA-N perchloric acid Chemical compound OCl(=O)(=O)=O VLTRZXGMWDSKGL-UHFFFAOYSA-N 0.000 description 2
- -1 potassium amino acid Chemical class 0.000 description 2
- 208000007645 potassium deficiency Diseases 0.000 description 2
- 239000012086 standard solution Substances 0.000 description 2
- 239000011573 trace mineral Substances 0.000 description 2
- 235000013619 trace mineral Nutrition 0.000 description 2
- BHPQYMZQTOCNFJ-UHFFFAOYSA-N Calcium cation Chemical compound [Ca+2] BHPQYMZQTOCNFJ-UHFFFAOYSA-N 0.000 description 1
- 241000196324 Embryophyta Species 0.000 description 1
- GRYLNZFGIOXLOG-UHFFFAOYSA-N Nitric acid Chemical compound O[N+]([O-])=O GRYLNZFGIOXLOG-UHFFFAOYSA-N 0.000 description 1
- 238000002835 absorbance Methods 0.000 description 1
- 238000010306 acid treatment Methods 0.000 description 1
- 230000009471 action Effects 0.000 description 1
- 239000003463 adsorbent Substances 0.000 description 1
- 230000002411 adverse Effects 0.000 description 1
- 238000001479 atomic absorption spectroscopy Methods 0.000 description 1
- 210000004369 blood Anatomy 0.000 description 1
- 239000008280 blood Substances 0.000 description 1
- 210000004204 blood vessel Anatomy 0.000 description 1
- 239000006227 byproduct Substances 0.000 description 1
- 229910001424 calcium ion Inorganic materials 0.000 description 1
- 235000012000 cholesterol Nutrition 0.000 description 1
- 238000007865 diluting Methods 0.000 description 1
- 208000002173 dizziness Diseases 0.000 description 1
- 230000037149 energy metabolism Effects 0.000 description 1
- 230000004438 eyesight Effects 0.000 description 1
- 239000012467 final product Substances 0.000 description 1
- 125000000524 functional group Chemical group 0.000 description 1
- 230000036541 health Effects 0.000 description 1
- 208000019622 heart disease Diseases 0.000 description 1
- 238000001727 in vivo Methods 0.000 description 1
- 230000002401 inhibitory effect Effects 0.000 description 1
- 229910052500 inorganic mineral Inorganic materials 0.000 description 1
- 210000004072 lung Anatomy 0.000 description 1
- 229920002521 macromolecule Polymers 0.000 description 1
- 238000000120 microwave digestion Methods 0.000 description 1
- 239000011707 mineral Substances 0.000 description 1
- 235000010755 mineral Nutrition 0.000 description 1
- 238000012986 modification Methods 0.000 description 1
- 230000004048 modification Effects 0.000 description 1
- 210000004165 myocardium Anatomy 0.000 description 1
- 230000002232 neuromuscular Effects 0.000 description 1
- 229910017604 nitric acid Inorganic materials 0.000 description 1
- 235000021049 nutrient content Nutrition 0.000 description 1
- 235000016709 nutrition Nutrition 0.000 description 1
- 230000035764 nutrition Effects 0.000 description 1
- 239000002245 particle Substances 0.000 description 1
- 230000035515 penetration Effects 0.000 description 1
- 150000003109 potassium Chemical class 0.000 description 1
- 239000001508 potassium citrate Substances 0.000 description 1
- 229960002635 potassium citrate Drugs 0.000 description 1
- QEEAPRPFLLJWCF-UHFFFAOYSA-K potassium citrate (anhydrous) Chemical compound [K+].[K+].[K+].[O-]C(=O)CC(O)(CC([O-])=O)C([O-])=O QEEAPRPFLLJWCF-UHFFFAOYSA-K 0.000 description 1
- 235000011082 potassium citrates Nutrition 0.000 description 1
- PHZLMBHDXVLRIX-UHFFFAOYSA-M potassium lactate Chemical compound [K+].CC(O)C([O-])=O PHZLMBHDXVLRIX-UHFFFAOYSA-M 0.000 description 1
- 239000001521 potassium lactate Substances 0.000 description 1
- 235000011085 potassium lactate Nutrition 0.000 description 1
- 229960001304 potassium lactate Drugs 0.000 description 1
- 230000009467 reduction Effects 0.000 description 1
- 239000011435 rock Substances 0.000 description 1
- 239000012488 sample solution Substances 0.000 description 1
- 210000002460 smooth muscle Anatomy 0.000 description 1
- 238000001179 sorption measurement Methods 0.000 description 1
- 238000006467 substitution reaction Methods 0.000 description 1
- 238000012360 testing method Methods 0.000 description 1
- 210000001519 tissue Anatomy 0.000 description 1
- 230000003313 weakening effect Effects 0.000 description 1
- 238000005303 weighing Methods 0.000 description 1
Classifications
-
- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
- A23L—FOODS, FOODSTUFFS, OR NON-ALCOHOLIC BEVERAGES, NOT COVERED BY SUBCLASSES A21D OR A23B-A23J; THEIR PREPARATION OR TREATMENT, e.g. COOKING, MODIFICATION OF NUTRITIVE QUALITIES, PHYSICAL TREATMENT; PRESERVATION OF FOODS OR FOODSTUFFS, IN GENERAL
- A23L33/00—Modifying nutritive qualities of foods; Dietetic products; Preparation or treatment thereof
- A23L33/10—Modifying nutritive qualities of foods; Dietetic products; Preparation or treatment thereof using additives
- A23L33/16—Inorganic salts, minerals or trace elements
-
- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
- A23L—FOODS, FOODSTUFFS, OR NON-ALCOHOLIC BEVERAGES, NOT COVERED BY SUBCLASSES A21D OR A23B-A23J; THEIR PREPARATION OR TREATMENT, e.g. COOKING, MODIFICATION OF NUTRITIVE QUALITIES, PHYSICAL TREATMENT; PRESERVATION OF FOODS OR FOODSTUFFS, IN GENERAL
- A23L5/00—Preparation or treatment of foods or foodstuffs, in general; Food or foodstuffs obtained thereby; Materials therefor
- A23L5/30—Physical treatment, e.g. electrical or magnetic means, wave energy or irradiation
-
- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
- A23L—FOODS, FOODSTUFFS, OR NON-ALCOHOLIC BEVERAGES, NOT COVERED BY SUBCLASSES A21D OR A23B-A23J; THEIR PREPARATION OR TREATMENT, e.g. COOKING, MODIFICATION OF NUTRITIVE QUALITIES, PHYSICAL TREATMENT; PRESERVATION OF FOODS OR FOODSTUFFS, IN GENERAL
- A23L5/00—Preparation or treatment of foods or foodstuffs, in general; Food or foodstuffs obtained thereby; Materials therefor
- A23L5/30—Physical treatment, e.g. electrical or magnetic means, wave energy or irradiation
- A23L5/32—Physical treatment, e.g. electrical or magnetic means, wave energy or irradiation using phonon wave energy, e.g. sound or ultrasonic waves
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12P—FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE
- C12P13/00—Preparation of nitrogen-containing organic compounds
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12P—FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE
- C12P7/00—Preparation of oxygen-containing organic compounds
- C12P7/40—Preparation of oxygen-containing organic compounds containing a carboxyl group including Peroxycarboxylic acids
- C12P7/44—Polycarboxylic acids
- C12P7/48—Tricarboxylic acids, e.g. citric acid
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12P—FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE
- C12P7/00—Preparation of oxygen-containing organic compounds
- C12P7/40—Preparation of oxygen-containing organic compounds containing a carboxyl group including Peroxycarboxylic acids
- C12P7/56—Lactic acid
-
- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
- A23V—INDEXING SCHEME RELATING TO FOODS, FOODSTUFFS OR NON-ALCOHOLIC BEVERAGES AND LACTIC OR PROPIONIC ACID BACTERIA USED IN FOODSTUFFS OR FOOD PREPARATION
- A23V2002/00—Food compositions, function of food ingredients or processes for food or foodstuffs
Abstract
The invention belongs to the technical field of potassium element extraction, and particularly relates to a method for extracting potassium element from beef tripe, which comprises the following steps: (1) carrying out magnetization treatment; (2) ball milling treatment; (3) degreasing; (4) high-voltage pulse electric field treatment; (5) carrying out centrifugal separation; (6) drying; the method of the invention efficiently converts potassium ions, improves the absorption utilization rate and the dissolution rate of potassium element, effectively destroys stable protein structure, and has good separation effect and simple steps.
Description
Technical Field
The invention belongs to the technical field of potassium element extraction, and particularly relates to a method for extracting potassium element from beef tripe.
Background
The beef tripe, i.e. the beef stomach, is one of the byproducts of cattle. The cattle have four stomachs, namely abomasum, reticulum, rumen and omasum. The rumen, reticulum and omasum are formed by the esophageal variation of cattle, and the abomasum is the true stomach. Soaking tripe in alkaline water to obtain a dish; meanwhile, the famous dishes such as 'cow tripe chafing dish' and 'husband and wife lung tablet' are all eaten by tearing off raw slices of cow serosa by using rumen. The application of the reticulum is the same as that of the rumen, and the omasum is also called as tripe stomach because a plurality of blades are arranged inside the omasum and are arranged alternately. The omasum and the abomasum can be cut into shreds to be eaten as dishes. The cattle tripe is mainly applied to the tripe collar and the tripe, the main protein of the cattle tripe is collagen, the collagen has a unique triple helix structure, and the structure can keep the molecular structure of the collagen stable; the beef tripe is crisp and tasty and has rich nutrition, and the nutrient content of the beef tripe is shown in a table 1:
TABLE 1
The beef tripe contains abundant trace elements, particularly potassium element, the potassium element is mostly extracted from potassium-containing rocks or plants at present, and the literature report that the potassium element is extracted from the beef tripe is not available temporarily, the potassium is a mineral substance necessary for human health, can maintain water and acid balance in blood and tissue cells, is an important element for maintaining the acid-base balance of human bodies, and participates in energy metabolism and maintains the normal function of neuromuscular. When potassium deficiency occurs in vivo, the smooth muscle of cardiac muscle or blood vessel is weakened, which causes general weakness, fatigue, heart beat weakening, dizziness and dim eyesight, and serious potassium deficiency may also cause heart diseases.
Disclosure of Invention
The invention provides a method for extracting potassium element from beef tripe to solve the technical problems.
The method is realized by the following technical scheme:
a method for extracting potassium element from beef tripe comprises the following steps:
(1) magnetization treatment: placing the beef tripe in a constant-intensity magnetic field with the magnetic field intensity of 1-7T for treating for 20-30 min;
(2) ball milling treatment: mixing magnetized beef tripe with 30-40% organic acid solution according to the weight ratio of 1: (3-5) grinding the mixture in a ball mill at the speed of 300-500 r/min for 10-20min to obtain beef tripe slurry;
(3) degreasing: adding Bacillus subtilis activation solution 20-30% of the weight of the beef tripe pulp into the beef tripe pulp, performing shake fermentation culture for 2-4h at 25-35 deg.C under the condition of 100 revolutions per minute, and filtering to obtain fermented beef tripe pulp;
(4) high-voltage pulse electric field treatment: placing the fermented beef tripe pulp in a high-voltage pulse electric field with the pulse voltage of 3-9kV and the pulse frequency of 40-70Hz for processing for 60-90 s;
(5) centrifugal separation: adding modified charcoal with a mass of 0.1-0.5% of the beef tripe pulp into the beef tripe pulp treated by the high-voltage pulse electric field, filtering, placing the filtrate in a microwave instrument, adding a citric acid solution into the microwave instrument to adjust the pH value to 3-6, stirring at a speed of 5000 plus materials and 10000 r/min for 5-10min, and taking the supernatant;
(6) and (3) drying: and drying the supernatant to obtain the potassium element.
The organic acid solution comprises, by mass, 13-23% of oxalic acid, 0.1-0.5% of lithium manganate and the balance of acetic acid solution.
The lithium manganate is processed for 5-10s under a constant-intensity magnetic field with the magnetic field intensity of 3-8T, and then is processed for 3-8s under a constant-intensity magnetic field with the magnetic field intensity of 10-13T.
Activating bacillus subtilis freeze-dried powder for 1-3 hours at 30-35 ℃ by using a culture medium containing a lactobacillus extract to obtain a suspension; adding carbonyl iron powder with the mass of 0.1-0.3% of the suspension into the suspension, uniformly stirring, and carrying out ultrasonic treatment for 10-25 min; the total viable count of the Bacillus subtilis activating solution is (5.6-9.6) multiplied by 108cfu/mL。
The working conditions of the ultrasonic wave are as follows: frequency 40-50kHz, power meter 100-140W.
The culture medium formula containing the lactobacillus extract comprises the following components: 10-12g/L of peptone, 7-9g/L of lactobacillus extract, 1-2g/L of potassium dihydrogen phosphate, 1-2g/L of triammonium citrate, 5-8g/L of sodium acetate, 20-25g/L of glucose, 801-2mL/L, MgSO4 & 7H2O0.2-0.45g/L of tween-801, 20-25g/L of agar and 2-3g/L of calcium chloride.
The preparation method of the lactobacillus extract comprises the following steps:
step 1: mixing lactobacillus with water according to the proportion of 1: (2-5) mixing, and stirring at the speed of 1000-2000 rpm for 20-25 min;
step 2: heating the product obtained in the step 1 to 80-90 ℃ at the speed of 1-3 ℃/min, carrying out heat preservation treatment for 10-20min, and freezing for 1-3h at the temperature of 0-4 ℃;
and step 3: and (3) placing the substance obtained in the step (2) under the conditions of power of 300-.
The modified biochar comprises the following components in parts by weight: 10-30 parts of coconut shell powder and 5-15 parts of peanut shell powder.
The preparation method of the modified biochar comprises the following steps: uniformly mixing coconut shell and peanut shell powder, heating to 60-80 ℃, carrying out heat preservation treatment for 10-15min, and then adding a soaking solution with the mass concentration of 30-50% into the mixture until the material-liquid ratio is 1: (3-7), stirring by using a stirrer, reacting for 30-45min at the temperature of 58-78 ℃ to obtain a mixture, drying the mixture, transferring the mixture into an atmosphere furnace for carbonization, introducing protective gas into the atmosphere furnace, heating to 15-30 ℃ per minute, raising the temperature to 300-500 ℃ and keeping for 1-3h to obtain the biochar.
The soaking solution is prepared from calcium chloride and cyclodextrin according to a mass ratio of 1: (2-4) mixing, and adding water to prepare a solution with the mass concentration of 30-50%.
The microwave treatment is carried out for 90-180s under the condition of 120-180MHz, and then for 200-300s under the condition of increasing the frequency to 300-400 MHz.
Has the advantages that:
the method of the invention efficiently converts potassium ions, improves the absorption utilization rate and the dissolution rate of potassium element, effectively destroys stable protein structure, and has good separation effect and simple steps.
According to the invention, the stable structure of protein can be damaged by organic acid treatment, especially, the lithium manganate added in the organic acid can catalyze the effect of oxalic acid, and meanwhile, the lithium manganate is matched with the magnetized beef tripe, so that the penetration of the organic acid can be promoted, the dissolution rate is further increased, and the dissolution effect is improved.
According to the invention, a large amount of insoluble potassium is converted into free potassium elements such as potassium lactate and potassium amino acid by using the action of bacillus subtilis, and the carbonyl iron powder is used for inhibiting Maillard reaction, so that the adverse effect of components such as glucose on potassium extraction is weakened, more importantly, the magnetic effect formed by a high-voltage pulse electric field can be absorbed to form magnetic reflection, further, the structure can be damaged, the dissolution rate can be improved, and the carbonyl iron powder can also improve the adsorption effect of modified biochar, so that the potassium elements are separated from other substances; the lactobacillus is used for activating the bacillus subtilis, so that the activity and the adaptability of the bacillus subtilis are enhanced, and the bacillus subtilis is not influenced by magnetized beef tripe to cause activity reduction. And the multi-frequency microwave reaction is combined, so that citric acid and potassium element can act to produce potassium citrate, and meanwhile, cyclodextrin wraps calcium ions and other trace elements, and macromolecular substances are precipitated quickly, so that quick and effective separation is realized.
The invention utilizes coconut shell powder and peanut shell powder as adsorbents, the biochar has small particle size, larger specific surface area and a microporous structure, and the surface has active functional groups, so that cholesterol and protein are wrapped in the biochar.
Detailed Description
The following is a detailed description of the embodiments of the present invention, but the present invention is not limited to these embodiments, and any modifications or substitutions in the basic spirit of the embodiments are included in the scope of the present invention as claimed in the claims.
Example 1
A method for extracting potassium element from beef tripe comprises the following steps:
(1) magnetization treatment: placing the beef tripe in a constant-intensity magnetic field with the magnetic field intensity of 7T for treating for 30 min;
(2) ball milling treatment: mixing magnetized beef tripe with 40% organic acid solution according to the weight ratio of 1: 5, grinding the mixture in a ball mill at the speed of 500 revolutions per minute for 20min to obtain beef tripe pulp;
(3) degreasing: adding 30% Bacillus subtilis activation solution by mass of Gaster bovis Seu Bubali pulp into Gaster bovis Seu Bubali pulp, performing shake fermentation at 35 deg.C and 200 r/min for 4 hr, and filtering to obtain fermented Gaster bovis Seu Bubali pulp;
(4) high-voltage pulse electric field treatment: placing the fermented beef tripe pulp in a high-voltage pulse electric field with the pulse voltage of 9kV and the pulse frequency of 70Hz for processing for 90 s;
(5) centrifugal separation: adding modified biochar 0.5% of the beef tripe pulp mass into the beef tripe pulp treated by the high-voltage pulse electric field, filtering, placing the filtrate into a microwave instrument, adding citric acid solution into the filtrate to adjust the pH value to 6, stirring at the speed of 10000 r/min for 10min, and taking supernatant;
(6) and (3) drying: drying the supernatant to obtain potassium element;
the organic acid solution comprises 23% of oxalic acid, 0.5% of lithium manganate and the balance of acetic acid solution in percentage by mass;
treating the lithium manganate for 10s under a constant-intensity magnetic field with the magnetic field intensity of 8T, and then treating for 8s under a constant-intensity magnetic field with the magnetic field intensity of 13T;
the bacillus subtilis activating solution is used for activating bacillus subtilis freeze-dried powder for 3 hours at 35 ℃ by adopting a culture medium containing a lactobacillus extract to obtain a suspension; adding carbonyl iron powder with the mass of 0.3 percent of the suspension into the suspension, uniformly stirring, and carrying out ultrasonic treatment for 25 min; the total viable count of the bacillus subtilis activating solution is 9.6 multiplied by 108cfu/mL；
The working conditions of the ultrasonic wave are as follows: frequency 50kHz, power meter 140W;
the culture medium formula containing the lactobacillus extract comprises the following components: peptone 12g/L, lactobacillus extract 9g/L, potassium dihydrogen phosphate 2g/L, triammonium citrate 2g/L, sodium acetate 5-8g/L, glucose 25g/L, Tween-802 mL/L, MgSO 4.7 H2O0.45g/L, agar 25g/L, calcium chloride 3 g/L;
the preparation method of the lactobacillus extract comprises the following steps:
step 1: mixing lactobacillus with water according to the proportion of 1: 5, and stirring at a speed of 2000 rpm for 25 min;
step 2: heating the product obtained in the step 1 to 90 ℃ at the speed of 3 ℃/min, carrying out heat preservation treatment for 20min, and freezing for 3h at the temperature of 4 ℃;
and step 3: placing the substance obtained in the step 2 under the conditions of 500W power and 80MHz microwave treatment for 20s, then ultrasonic wave treatment under the conditions of 800W power and 64kHz ultrasonic wave treatment for 300s, then centrifugally separating, and concentrating or spray drying the separated liquid to obtain the yeast extract;
the modified biochar comprises the following components in parts by weight: 30 parts of coconut shell powder and 15 parts of peanut shell powder;
the preparation method of the modified biochar comprises the following steps: uniformly mixing coconut shell parts and peanut shell powder, heating to 80 ℃, carrying out heat preservation treatment for 15min, and then adding a soaking solution with the mass concentration of 50% into the mixture until the material-liquid ratio is 1: 7, stirring by using a stirrer, reacting for 45min at the temperature of 78 ℃ to obtain a mixture, drying the mixture, transferring the mixture into an atmosphere furnace for carbonization, wherein protective gas is introduced into the atmosphere furnace, and the temperature is increased to 3500 ℃ by raising the temperature by 30 ℃ per minute and keeping the temperature for 3h to obtain biochar;
the soaking solution is prepared from calcium chloride and cyclodextrin according to a mass ratio of 1: 4 mixing, adding water to prepare a solution with the mass concentration of 50%;
the microwave treatment is to treat for 180s under the condition of 180MHz firstly and then treat for 300s under the condition of increasing the frequency to 400 MHz.
Example 2
A method for extracting potassium element from beef tripe comprises the following steps:
(1) magnetization treatment: placing the beef tripe in a constant-intensity magnetic field with the magnetic field intensity of 1T for treating for 20 min;
(2) ball milling treatment: mixing magnetized beef tripe with 30% organic acid solution according to the weight concentration ratio of 1: 3, grinding the mixture in a ball mill at the speed of 300 revolutions per minute for 10min to obtain beef tripe pulp;
(3) degreasing: adding Bacillus subtilis activation solution 20% of the weight of the cattle tripe pulp into the cattle tripe pulp, performing shake fermentation culture at 25 deg.C and 100 r/min for 2h, and filtering to obtain fermented cattle tripe pulp;
(4) high-voltage pulse electric field treatment: placing the fermented beef tripe pulp in a high-voltage pulse electric field with the pulse voltage of 3kV and the pulse frequency of 40Hz for processing for 60 s;
(5) centrifugal separation: adding modified biochar 0.1% of the beef tripe pulp mass into the beef tripe pulp treated by the high-voltage pulse electric field, filtering, placing the filtrate into a microwave instrument, adding citric acid solution to adjust the pH to 3, stirring at the speed of 5000 r/min for 5min, and taking supernatant;
(6) and (3) drying: drying the supernatant to obtain potassium element;
the organic acid solution comprises 13% of oxalic acid, 0.1% of lithium manganate and the balance of acetic acid solution in percentage by mass;
treating the lithium manganate for 5s under a constant-intensity magnetic field with the magnetic field intensity of 3T, and then treating for 3s under a constant-intensity magnetic field with the magnetic field intensity of 10T;
the bacillus subtilis activation solution is used for activating bacillus subtilis freeze-dried powder for 1 hour at the temperature of 30 ℃ by adopting a culture medium containing a lactobacillus extract to obtain a suspension; adding carbonyl iron powder with the mass of 0.1 percent of the suspension into the suspension, uniformly stirring, and carrying out ultrasonic treatment for 10 min; the total viable count of the bacillus subtilis activating solution is 5.6 multiplied by 108cfu/mL；
The working conditions of the ultrasonic wave are as follows: frequency 40kHz, power meter 100W;
the culture medium formula containing the lactobacillus extract comprises the following components: 10g/L of peptone, 7g/L of lactobacillus extract, 1g/L of potassium dihydrogen phosphate, 1g/L of triammonium citrate, 5g/L of sodium acetate, 20g/L of glucose, 20g/L of Tween-801 mL/L, MgSO 4.7H 2O0.2g/L, 20g/L of agar and 2g/L of calcium chloride;
the preparation method of the lactobacillus extract comprises the following steps:
step 1: mixing lactobacillus with water according to the proportion of 1: 2, stirring at the speed of 1000 revolutions per minute for 20 min;
step 2: heating the product obtained in the step 1 to 80 ℃ at the speed of 1 ℃/min, carrying out heat preservation treatment for 10min, and freezing for 1h at the temperature of 0 ℃;
and step 3: placing the substance obtained in the step 2 under the conditions of 300W power and 60MHz microwave treatment for 10s, then performing ultrasonic treatment for 180s under the conditions of 600W power and 45kHz frequency, then performing centrifugal separation, and concentrating or spray drying the separated liquid to obtain the yeast extract;
the modified biochar comprises the following components in parts by weight: 10 parts of coconut shell powder and 5 parts of peanut shell powder;
the preparation method of the modified biochar comprises the following steps: uniformly mixing coconut shell parts and peanut shell powder, heating to 60 ℃, carrying out heat preservation treatment for 10min, and then adding a soaking solution with the mass concentration of 30% into the mixture until the material-liquid ratio is 1: 3, stirring by using a stirrer, reacting for 30min at the temperature of 58 ℃ to obtain a mixture, drying the mixture, transferring the mixture into an atmosphere furnace for carbonization, wherein protective gas is introduced into the atmosphere furnace, the temperature is increased by 15 ℃ per minute, the temperature is increased to 300 ℃, and the temperature is kept for 1h to obtain biochar;
the soaking solution is prepared from calcium chloride and cyclodextrin according to a mass ratio of 1: 2 mixing, adding water to prepare a solution with the mass concentration of 30%;
the microwave treatment is to treat for 90s under the condition of 120MHz firstly and then to treat for 200s under the condition of increasing the frequency to 300 MHz.
Example 3
A method for extracting potassium element from beef tripe comprises the following steps:
(1) magnetization treatment: placing the beef tripe in a constant-intensity magnetic field with the magnetic field intensity of 4T for treatment for 25 min;
(2) ball milling treatment: mixing magnetized beef tripe with 35% organic acid solution according to the weight ratio of 1: 4, grinding for 15min in a ball mill at the speed of 400 revolutions per minute to obtain beef tripe pulp;
(3) degreasing: adding a bacillus subtilis activation solution accounting for 25% of the mass of the beef tripe pulp into the beef tripe pulp, performing shake fermentation culture for 3h at 30 ℃ and 150 r/min, and filtering to obtain fermented beef tripe pulp;
(4) high-voltage pulse electric field treatment: placing the fermented beef tripe pulp in a high-voltage pulse electric field with pulse voltage of 6kV and pulse frequency of 55Hz for processing for 75 s;
(5) centrifugal separation: adding modified biochar 0.3% of the beef tripe pulp mass into the beef tripe pulp treated by the high-voltage pulse electric field, filtering, placing the filtrate into a microwave instrument, adding citric acid solution into the filtrate to adjust the pH to 4.5, stirring for 8min at the speed of 8000 revolutions per minute, and taking supernatant;
(6) and (3) drying: drying the supernatant to obtain potassium element;
the organic acid solution comprises 18% of oxalic acid, 0.3% of lithium manganate and the balance of acetic acid solution in percentage by mass;
treating the lithium manganate for 8s under a constant-intensity magnetic field with the magnetic field intensity of 5T, and then treating for 5s under a constant-intensity magnetic field with the magnetic field intensity of 12T;
activating bacillus subtilis freeze-dried powder for 2 hours at 32 ℃ by using a culture medium containing a lactobacillus extract to obtain a suspension; adding carbonyl iron powder with the mass of 0.2 percent of the suspension into the suspension, uniformly stirring, and carrying out ultrasonic treatment for 20 min; the total viable count of the bacillus subtilis activating solution is 7.6 multiplied by 108cfu/mL；
The working conditions of the ultrasonic wave are as follows: frequency 45kHz, power meter 120W;
the culture medium formula containing the lactobacillus extract comprises the following components: 11g/L of peptone, 8g/L of lactobacillus extract, 1.5g/L of potassium dihydrogen phosphate, 1.5g/L of triammonium citrate, 6.5g/L of sodium acetate, 22.5g/L of glucose, 801.5mL/L, MgSO 4.7H 2O0.35g/L of tween-801.5, 22.5g/L of agar and 2.5g/L of calcium chloride;
the preparation method of the lactobacillus extract comprises the following steps:
step 1: mixing lactobacillus with water according to the proportion of 1: 3.5, and stirring at the speed of 1500 revolutions per minute for 22 min;
step 2: heating the product obtained in the step 1 to 85 ℃ at the speed of 2 ℃/min, carrying out heat preservation treatment for 15min, and freezing for 1-3h at the temperature of 2 ℃;
and step 3: placing the substance obtained in the step 2 under the conditions of 400W power and 70MHz microwave treatment for 15s, then performing ultrasonic treatment for 240s under the conditions of 700W power and 55kHz frequency, then performing centrifugal separation, and concentrating or spray drying the separated liquid to obtain the yeast extract;
the modified biochar comprises the following components in parts by weight: 15 parts of coconut shell powder and 10 parts of peanut shell powder;
the preparation method of the modified biochar comprises the following steps: uniformly mixing coconut shell parts and peanut shell powder, heating to 70 ℃, carrying out heat preservation treatment for 12min, and then adding a soaking solution with the mass concentration of 40% into the mixture until the material-liquid ratio is 1: 5, stirring by using a stirrer, reacting for 38min at the temperature of 68 ℃ to obtain a mixture, drying the mixture, transferring the mixture into an atmosphere furnace for carbonization, wherein protective gas is introduced into the atmosphere furnace, the temperature is increased to 400 ℃ at the rate of 20 ℃ per minute, and the temperature is maintained for 2h to obtain biochar;
the soaking solution is prepared from calcium chloride and cyclodextrin according to a mass ratio of 1: 3 mixing, adding water to prepare a solution with the mass concentration of 40%;
the microwave treatment is to treat for 130s under the condition of 150MHz firstly and then treat for 250s under the condition of increasing the frequency to 350 MHz.
Example 4
A method for extracting potassium element from beef tripe comprises the following steps:
(1) magnetization treatment: placing the beef tripe in a constant-intensity magnetic field with the magnetic field intensity of 4T for treating for 20 min;
(2) ball milling treatment: mixing magnetized beef tripe with 40% organic acid solution according to the weight ratio of 1: 4, grinding the mixture in a ball mill at a speed of 00 r/min for 10min to obtain beef tripe slurry;
(3) degreasing: adding Bacillus subtilis activation solution 20% of the weight of the cattle tripe pulp into the cattle tripe pulp, performing shake fermentation culture at 35 deg.C and 200 r/min for 2h, and filtering to obtain fermented cattle tripe pulp;
(4) high-voltage pulse electric field treatment: placing the fermented beef tripe pulp in a high-voltage pulse electric field with the pulse voltage of 9kV and the pulse frequency of 40Hz for processing for 75 s;
(5) centrifugal separation: adding modified biochar 0.5% of the beef tripe pulp mass into the beef tripe pulp treated by the high-voltage pulse electric field, filtering, placing the filtrate into a microwave instrument, adding citric acid solution to adjust the pH value to 5, stirring at the speed of 5000 r/min for 5min, and taking supernatant;
(6) and (3) drying: drying the supernatant to obtain potassium element;
the organic acid solution comprises, by mass, 20% of oxalic acid, 0.3% of lithium manganate and the balance of acetic acid solution;
treating the lithium manganate for 8s under a constant-intensity magnetic field with the magnetic field intensity of 6T, and then treating for 6s under a constant-intensity magnetic field with the magnetic field intensity of 11T;
activating bacillus subtilis freeze-dried powder for 2 hours at 34 ℃ by using a culture medium containing a lactobacillus extract to obtain a suspension; adding carbonyl iron powder with the mass of 0.2 percent of the suspension into the suspension, uniformly stirring, and carrying out ultrasonic treatment for 15 min; the total viable count of the bacillus subtilis activating solution is 7.6 multiplied by 108cfu/mL；
The working conditions of the ultrasonic wave are as follows: frequency 45kHz, power meter 120W;
the culture medium formula containing the lactobacillus extract comprises the following components: 11g/L of peptone, 8g/L of lactobacillus extract, 1g/L of potassium dihydrogen phosphate, 2g/L of triammonium citrate, 8g/L of sodium acetate, 20g/L of glucose, 25g/L of agar, and 2g/L of calcium chloride, wherein the Tween-802 mL/L, MgSO 4.7H 2O0.4 g/L;
the preparation method of the lactobacillus extract comprises the following steps:
step 1: mixing lactobacillus with water according to the proportion of 1: 3, and stirring at the speed of 1500 rpm for 23 min;
step 2: heating the product obtained in the step 1 to 85 ℃ at the speed of 2 ℃/min, carrying out heat preservation treatment for 15min, and freezing for 2h at the temperature of 3 ℃;
and step 3: placing the substance obtained in the step 2 under the conditions of 500W power and 70MHz microwave treatment for 15s, then performing ultrasonic treatment for 200s under the conditions of 700W power and 50kHz frequency, then performing centrifugal separation, and concentrating or spray drying the separated liquid to obtain the yeast extract;
the modified biochar comprises the following components in parts by weight: 20 parts of coconut shell powder and 12 parts of peanut shell powder;
the preparation method of the modified biochar comprises the following steps: uniformly mixing coconut shell parts and peanut shell powder, heating to 80 ℃, carrying out heat preservation treatment for 15min, and then adding a soaking solution with the mass concentration of 50% into the mixture until the material-liquid ratio is 1: 4, stirring by using a stirrer, reacting for 30min at the temperature of 65 ℃ to obtain a mixture, drying the mixture, transferring the mixture into an atmosphere furnace for carbonization, wherein protective gas is introduced into the atmosphere furnace, the temperature is increased by 20 ℃ per minute, the temperature is increased to 500 ℃, and the temperature is maintained for 3h to obtain biochar;
the soaking solution is prepared from calcium chloride and cyclodextrin according to a mass ratio of 1: 3 mixing, adding water to prepare a solution with the mass concentration of 50%;
the microwave treatment is to treat for 150s under the condition of 150MHz firstly and then treat for 250s under the condition of increasing the frequency to 300 MHz.
Comparative example 1
The difference from example 3 is that: the organic acid solution contains 20% of oxalic acid and the balance of acetic acid solution in percentage by mass.
Comparative example 2
The difference from example 3 is that: step (1) is not included.
Comparative example 3
The difference from example 3 is that: the modified biochar is replaced by biochar, and the biochar comprises the following components in parts by weight: 20 parts of coconut shell powder and 12 parts of peanut shell powder.
Test example 1 measurement of Potassium content
And (3) drawing a potassium standard curve, namely preparing a series of potassium element standard solutions with gradient concentrations of 0.2mg/L, 0.6mg/L, 1.0mg/L, 1.5mg/L and 2.0mg/L, drawing a K standard curve by taking the concentration of the standard solution as an abscissa and the absorbance as an ordinate, and determining a standard curve equation, a correlation coefficient and a linear range.
Weighing 0.1000g of samples obtained in the embodiment and the comparative example, respectively placing the samples in a digestion tank, adding 8mL of nitric acid, soaking for 24 hours, adding 2mL of hydrofluoric acid and 2mL of perchloric acid, digesting in a microwave digestion instrument under the digestion conditions of 1000W of power, 10min at 130 ℃, 5min at 150 ℃, 5min at 180 ℃ and 10min at 200 ℃, concentrating by acid after digestion, diluting to fix the volume and measuring the volume; the results of measurement by atomic absorption spectroscopy are shown in Table 2:
TABLE 2
From table 2, it can be seen that: the potassium content in the final product is as high as 97.5% by extracting according to the method of the embodiment, which shows that the purity is high.
Meanwhile, the lower layer and the biochar obtained after filtration are dried, and the content of potassium element is measured according to the method of preparing the sample solution and measuring potassium as shown in the table 3:
TABLE 3
| Item | Example 1 | Example 2 | Example 3 | Example 4 |
| Content (%) | 0.83 | 0.97 | 0.77 | 0.90 |
| Item | Comparative example 1 | Comparative example 2 | Comparative example 3 | |
| Content (%) | 7.8 | 12.6 | 4.5 |
From table 3, it can be seen that: the potassium extraction rate was high as the potassium residue remained less in other substances when the extraction was performed according to the method of example.
Claims (10)
1. A method for extracting potassium element from beef tripe is characterized by comprising the following steps:
(1) magnetization treatment: placing the beef tripe in a constant-intensity magnetic field with the magnetic field intensity of 1-7T for treating for 20-30 min;
(2) ball milling treatment: mixing magnetized beef tripe with 30-40% organic acid solution according to the weight ratio of 1: (3-5) grinding the mixture in a ball mill at the speed of 300-500 r/min for 10-20min to obtain beef tripe slurry;
(3) degreasing: adding Bacillus subtilis activation solution 20-30% of the weight of the beef tripe pulp into the beef tripe pulp, performing shake fermentation culture for 2-4h at 25-35 deg.C under the condition of 100 revolutions per minute, and filtering to obtain fermented beef tripe pulp;
(4) high-voltage pulse electric field treatment: placing the fermented beef tripe pulp in a high-voltage pulse electric field with the pulse voltage of 3-9kV and the pulse frequency of 40-70Hz for processing for 60-90 s;
(5) centrifugal separation: adding modified charcoal with a mass of 0.1-0.5% of the beef tripe pulp into the beef tripe pulp treated by the high-voltage pulse electric field, filtering, placing the filtrate in a microwave instrument, adding a citric acid solution into the microwave instrument to adjust the pH value to 3-6, stirring at a speed of 5000 plus materials and 10000 r/min for 5-10min, and taking the supernatant;
(6) and (3) drying: and drying the supernatant to obtain the potassium element.
2. The method for extracting potassium element from beef tripe according to claim 1, wherein the organic acid solution comprises 13-23% by mass of oxalic acid, 0.1-0.5% by mass of lithium manganate, and the balance of acetic acid solution.
3. The method for extracting potassium element from beef tripe according to claim 2, wherein the lithium manganate is treated under a constant intensity magnetic field with a magnetic field strength of 3-8T for 5-10s, and then treated under a constant intensity magnetic field with a magnetic field strength of 10-13T for 3-8 s.
4. The method for extracting potassium element from beef tripe according to claim 1, wherein the bacillus subtilis activation solution is prepared by activating bacillus subtilis freeze-dried powder for 1-3 hours at 30-35 ℃ by using a culture medium containing lactobacillus extract to obtain a suspension; adding carbonyl iron powder with the mass of 0.1-0.3% of the suspension into the suspension, uniformly stirring, and carrying out ultrasonic treatment for 10-25 min; the total viable count of the Bacillus subtilis activating solution is (5.6-9.6) multiplied by 108cfu/mL。
5. The method for extracting potassium element from beef tripe as claimed in claim 4, wherein the working conditions of the ultrasonic wave are as follows: frequency 40-50kHz, power meter 100-140W.
6. The method for extracting potassium element from beef tripe according to claim 1, wherein the culture medium formula of the lactobacillus extract comprises: 10-12g/L of peptone, 7-9g/L of lactobacillus extract, 1-2g/L of potassium dihydrogen phosphate, 1-2g/L of triammonium citrate, 5-8g/L of sodium acetate, 20-25g/L of glucose, 801-2mL/L, MgSO4 & 7H2O0.2-0.45g/L of tween-801, 20-25g/L of agar and 2-3g/L of calcium chloride.
7. The method for extracting potassium element from tripe of cow as claimed in claim 6, wherein the lactobacillus extract is prepared by the following steps:
step 1: mixing lactobacillus with water according to the proportion of 1: (2-5) mixing, and stirring at the speed of 1000-2000 rpm for 20-25 min;
step 2: heating the product obtained in the step 1 to 80-90 ℃ at the speed of 1-3 ℃/min, carrying out heat preservation treatment for 10-20min, and freezing for 1-3h at the temperature of 0-4 ℃;
and step 3: and (3) placing the substance obtained in the step (2) under the conditions of power of 300-.
8. The method for extracting potassium element from beef tripe according to claim 1, wherein the modified biochar comprises the following components in parts by weight: 10-30 parts of coconut shell powder and 5-15 parts of peanut shell powder.
9. The method for extracting potassium element from beef tripe according to claim 1, wherein the modified biochar is prepared by the following steps: uniformly mixing coconut shell and peanut shell powder, heating to 60-80 ℃, carrying out heat preservation treatment for 10-15min, and then adding a soaking solution with the mass concentration of 30-50% into the mixture until the material-liquid ratio is 1: (3-7), stirring by using a stirrer, reacting for 30-45min at the temperature of 58-78 ℃ to obtain a mixture, drying the mixture, transferring the mixture into an atmosphere furnace for carbonization, introducing protective gas into the atmosphere furnace, heating to 15-30 ℃ per minute, raising the temperature to 300-500 ℃ and keeping for 1-3h to obtain the biochar.
10. The method as claimed in claim 9, wherein the microwave treatment is performed under the conditions of 180MHz at 120-.
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Application publication date: 20200207 |