CN110754471B - Insecticidal activity of AMEP412 protein on trialeurodes vaporariorum and application of insecticidal activity to trialeurodes vaporariorum - Google Patents
Insecticidal activity of AMEP412 protein on trialeurodes vaporariorum and application of insecticidal activity to trialeurodes vaporariorum Download PDFInfo
- Publication number
- CN110754471B CN110754471B CN201911212945.9A CN201911212945A CN110754471B CN 110754471 B CN110754471 B CN 110754471B CN 201911212945 A CN201911212945 A CN 201911212945A CN 110754471 B CN110754471 B CN 110754471B
- Authority
- CN
- China
- Prior art keywords
- protein
- amep412
- trialeurodes vaporariorum
- insecticidal activity
- application
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Active
Links
Images
Classifications
-
- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01N—PRESERVATION OF BODIES OF HUMANS OR ANIMALS OR PLANTS OR PARTS THEREOF; BIOCIDES, e.g. AS DISINFECTANTS, AS PESTICIDES OR AS HERBICIDES; PEST REPELLANTS OR ATTRACTANTS; PLANT GROWTH REGULATORS
- A01N37/00—Biocides, pest repellants or attractants, or plant growth regulators containing organic compounds containing a carbon atom having three bonds to hetero atoms with at the most two bonds to halogen, e.g. carboxylic acids
- A01N37/44—Biocides, pest repellants or attractants, or plant growth regulators containing organic compounds containing a carbon atom having three bonds to hetero atoms with at the most two bonds to halogen, e.g. carboxylic acids containing at least one carboxylic group or a thio analogue, or a derivative thereof, and a nitrogen atom attached to the same carbon skeleton by a single or double bond, this nitrogen atom not being a member of a derivative or of a thio analogue of a carboxylic group, e.g. amino-carboxylic acids
- A01N37/46—N-acyl derivatives
-
- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01N—PRESERVATION OF BODIES OF HUMANS OR ANIMALS OR PLANTS OR PARTS THEREOF; BIOCIDES, e.g. AS DISINFECTANTS, AS PESTICIDES OR AS HERBICIDES; PEST REPELLANTS OR ATTRACTANTS; PLANT GROWTH REGULATORS
- A01N63/00—Biocides, pest repellants or attractants, or plant growth regulators containing microorganisms, viruses, microbial fungi, animals or substances produced by, or obtained from, microorganisms, viruses, microbial fungi or animals, e.g. enzymes or fermentates
- A01N63/20—Bacteria; Substances produced thereby or obtained therefrom
- A01N63/22—Bacillus
-
- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01N—PRESERVATION OF BODIES OF HUMANS OR ANIMALS OR PLANTS OR PARTS THEREOF; BIOCIDES, e.g. AS DISINFECTANTS, AS PESTICIDES OR AS HERBICIDES; PEST REPELLANTS OR ATTRACTANTS; PLANT GROWTH REGULATORS
- A01N63/00—Biocides, pest repellants or attractants, or plant growth regulators containing microorganisms, viruses, microbial fungi, animals or substances produced by, or obtained from, microorganisms, viruses, microbial fungi or animals, e.g. enzymes or fermentates
- A01N63/50—Isolated enzymes; Isolated proteins
-
- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01P—BIOCIDAL, PEST REPELLANT, PEST ATTRACTANT OR PLANT GROWTH REGULATORY ACTIVITY OF CHEMICAL COMPOUNDS OR PREPARATIONS
- A01P7/00—Arthropodicides
- A01P7/04—Insecticides
Landscapes
- Life Sciences & Earth Sciences (AREA)
- Zoology (AREA)
- Engineering & Computer Science (AREA)
- Health & Medical Sciences (AREA)
- General Health & Medical Sciences (AREA)
- Environmental Sciences (AREA)
- Wood Science & Technology (AREA)
- Pest Control & Pesticides (AREA)
- Plant Pathology (AREA)
- Agronomy & Crop Science (AREA)
- Dentistry (AREA)
- Virology (AREA)
- Microbiology (AREA)
- Biotechnology (AREA)
- Proteomics, Peptides & Aminoacids (AREA)
- Insects & Arthropods (AREA)
- Chemical & Material Sciences (AREA)
- Chemical Kinetics & Catalysis (AREA)
- General Chemical & Material Sciences (AREA)
- Agricultural Chemicals And Associated Chemicals (AREA)
- Peptides Or Proteins (AREA)
Abstract
The invention relates to application of bacillus subtilis protein AMEP412 in controlling trialeurodes vaporariorum. The invention discovers the insecticidal activity of the AMEP412 protein on trialeurodes vaporariorum and the application thereof in controlling the trialeurodes vaporariorum, the protein can kill the trialeurodes vaporariorum in low concentration, has the stability of heat resistance and natural degradation resistance, can be taken into intestinal tracts by the trialeurodes vaporariorum, and does not inhibit the activity of protease in the intestinal tracts; in addition, the AMEP412 sprayed in the greenhouse has good control effect on the trialeurodes vaporariorum; the invention accumulates new materials for the biological control work of the trialeurodes vaporariorum.
Description
Technical Field
The invention belongs to the field of plant protection and biological control, and relates to insecticidal activity of AMEP412 protein on trialeurodes vaporariorum and application of the insecticidal activity to the trialeurodes vaporariorum.
Background
Whitefly (Bemisia tabaci) is an important pest of various crops such as vegetables, cotton, ornamental plants, and the like. It damages crops by eating plant stem and leaf juice and spreading plant viruses, resulting in huge losses in agricultural production and national economy. The main strategy for preventing and treating powdery mildew is mainly chemical insecticide. However, whiteflies develop resistance to those insecticides which are used excessively and frequently. In view of the above, the search for novel insecticides should become a new research focus.
Compared with chemical pesticides, proteins with insecticidal activity have relatively low probability of resisting insects due to different action modes, and become a hotspot of current pesticide research. There have been many reports on the use of pesticidal proteins in pest control. The best known example is the Cry toxin of Bacillus thuringiensis (Bt), which was developed into Bt transgenic cotton and effectively controls lepidopteran pest larvae. The wide planting of the Bt transgenic cotton greatly reduces the use of chemical pesticides. However, Cry toxins are not effective against trialeurodes vaporariorum. In recent years, some researchers have focused on screening for insecticidal proteins from plants that are rarely infested with trialeurodes vaporariorum. Das et al, 2009, reported that a mannose-binding lectin, extracted from garlic leaves, was effective in inhibiting whitefly. Jin et al expressed the lectin of pinellia ternata in chloroplasts in 2012 and demonstrated its ability to resist trialeurodes vaporariorum. Shukla et al identified a pesticidal protein (Tma12) from an edible fern in 2016 and expressed in transgenic cotton, which protein exhibited some resistance to white whitefly. Although these insecticidal proteins from plants have some potential for controlling whitefly, they are not widely used at present due to low extraction rates and limited application of transgenes.
In previous researches, the protein AMEP412 is separated and identified from the bacillus subtilis BU412, can stimulate defense response of plants, improve disease resistance of the plants, can generate antagonism on streptomyces scabies, and is an ideal candidate for developing biological pesticides. In the present invention, we aimed to provide a novel use of the AMEP412 protein.
Disclosure of Invention
The invention aims to provide a new application of bacillus subtilis protein AMEP412, which solves the problem that the existing insecticidal protein for trialeurodes vaporariorum is too little and solves the problem of green prevention and control of the trialeurodes vaporariorum.
The invention is realized by the following technical scheme: an application of Bacillus subtilis protein AMEP412 in controlling whitefly.
The amino acid sequence of the Bacillus subtilis protein AMEP412 is shown in SEQ ID NO. 1.
Furthermore, after the AMEP412 protein is fed to whitefly adults, the protein concentration required for achieving half lethal dose is 15.57 mu g/ml, and the protein concentration required for achieving 90% lethal rate is 63.27 mu g/ml.
Further, the AMEP412 protein can be ingested by the whitefly adults into the body, and is intensively positioned in the intestinal tract.
Further, the AMEP412 protein has heat-resistant stability.
Further, the AMEP412 protein has stability to natural degradation.
Further, the AMEP412 protein is sprayed in a greenhouse at a concentration of 100 mu g/ml, and the fatality rate of trialeurodes vaporariorum after 2 days is 56%.
Firstly, AMEP412 protein with different concentration gradients is used for artificially feeding the white fly imagoes, and the half fatality rate (LC) is calculated50) And protein concentration required for 90% lethality. And then carrying out heat treatment and natural degradation treatment on the AMEP412 protein, detecting the insecticidal activity of the treated protein sample on the white whitefly imagoes, and determining the stability of the AMEP412 protein. And (3) marking AMEP412 protein by using FITC, observing the fluorescence distribution in the trialeurodes vaporariorum by using a fluorescence microscope after feeding the adult trialeurodes vaporariorum, and determining the location of the AMEP412 in the trialeurodes vaporariorum. The inhibition of trypsin by AMEP412 was tested, excluding the possibility of killing whitefly by inhibiting its intestinal protease activity.
Adopt above-mentioned technical scheme's positive effect: the invention discovers the insecticidal activity of the AMEP412 protein on trialeurodes vaporariorum and the application thereof in controlling the trialeurodes vaporariorum, the protein can kill the trialeurodes vaporariorum in low concentration, has the stability of heat resistance and natural degradation resistance, can be taken into intestinal tracts by the trialeurodes vaporariorum, and does not inhibit the activity of protease in the intestinal tracts; in addition, the AMEP412 sprayed in the greenhouse has good control effect on the trialeurodes vaporariorum; the invention accumulates new materials for the biological control work of the trialeurodes vaporariorum.
Drawings
Fig. 1 is a graph of mortality of different concentrations of the AMEP412 protein to whitefly adults, with the same letters indicating no significant difference and different letters indicating significant differences between treatments (α ═ 5%);
fig. 2 is a graph showing the lethality of heat-treated and naturally degraded AMEP412 protein to whitefly adults, CK indicates that no AMEP412 protein was added, and unored indicates that the AMEP412 protein was not treated, the same letters indicate no significant difference, and different letters indicate significant difference between treatments (α ═ 5%);
fig. 3 is a fluorescent localization of FITC-labeled AMEP412 in whitefly adults, with bright spots in the abdominal cavity as excited green fluorescence, showing that AMEP412 is localized in the gut.
Detailed Description
The present invention is further described below by way of examples, it being understood that these examples are for illustrative purposes only and do not limit the scope of the present invention in any way.
Sources of the biological material in the present invention:
1. the used Bacillus subtilis BU412 is preserved in the China center for type culture Collection in 2016, 3, 30 days, with the preservation number of CCTCC M2016142;
2. AMEP412 protein: patent application No. 201810928176.1 entitled "identification of a novel function of Bacillus subtilis AMEP412 protein and its antibacterial peptide", application date 20180814, publication date 20181228, publication No. CN 109096379A.
Example 1
This example illustrates the insecticidal effect of Bacillus subtilis AMEP412 protein when artificially fed to Bemisia alba.
The liquid artificial bait for feeding is prepared by dissolving 5% yeast extract and 30% sucrose in distilled water, sterilizing, adding AMEP412(1, 5, 10, 20, 40 and 80 μ g/ml) with different concentrations, and mixing. Artificial bait without AMEP412 was used as a control. 1-2 days old adult trialeurodes vaporariorum was expelled from the plant leaves into 50ml test tubes, and at least 50 adult trialeurodes vaporariorum were taken per test tube. Two layers of stretched sealing films were applied to the test tube cap, bait (100 μ l) was added between the two films, and then the cap was reversed over the cap to keep the double-layered structure inside the test tube and placed upright. Each set of experiments was repeated three times. Mortality was determined after 2 days of feeding and was recorded by counting dead whitefly adults at the bottom of the tubes. Data were analyzed using one-way analysis of variance (ANOVA) and compared using Tukey's-HSD test (α ═ 5%). The half lethal concentration (LC50) and the 90% lethal concentration were calculated by probit analysis of the SPSS program.
The mortality rate of trialeurodes vaporariorum was counted after 2 days of feeding as shown in fig. 1. With the increase of the AMEP412 protein concentration, the mortality rate of the trialeurodes vaporariorum is gradually increased and shows a positive correlation trend. The mortality rates of trialeurodes vaporariorum corresponding to the AMEP412 protein concentrations of 0, 1, 5, 10, 20, 40, 80mg/ml were 7.2%, 17.4%, 31.0%, 47.9%, 65.5%, 82.7% and 95.6%, respectively. The software calculated the AMEP412 protein concentration required to achieve half-lethal rate was 15.57. mu.g/ml (Table 1). In addition, the AMEP412 protein concentration required to achieve 90% lethality was 63.27 μ g/ml. These results indicate that the AMEP412 protein has insecticidal activity against the whitefly adults, and the required half-lethal concentration is low, indicating that the insecticidal activity is strong.
TABLE 1 calculation of the median lethal concentration of AMEP412 protein
Example 2
This example illustrates the stability experiments of the Bacillus subtilis AMEP412 protein.
To test the thermotolerance of the AMEP412 protein, the protein samples were treated at 95 ℃ for 15 minutes and 30 minutes, respectively. The cooled protein sample was used for preparing artificial bait according to the method of example 1, and the insecticidal effect of trialeurodes vaporariorum was tested by adjusting the final concentration to 60. mu.g/ml. Untreated protein samples were used as controls, and each treatment was repeated three times. To test the stability of the AMEP412 protein to natural degradation, protein samples were placed in 1.5ml centrifuge tubes and left to stand at 25 ℃ for 24 hours and 48 hours, respectively, at room temperature. Then, the protein sample was used to prepare artificial bait according to the method of example 1, and the final concentration was adjusted to 60. mu.g/ml to test the insecticidal effect of Bemisia alba. Untreated protein samples were used as controls, and each treatment was repeated three times. The mortality data for trialeurodes vaporariorum above were compared using one-way anova using Tukey's HSD test (α ═ 5%).
The results show (fig. 2) that the mortality of aleurodes vaporariorum is reduced by 0.57% and 0.49% respectively by the treatment of the AMEP412 protein at 95 ℃ for 15 minutes and 30 minutes, compared with the AMEP412 protein without heat treatment, and the difference is not significant. Indicating that AMEP412 has good thermal stability. Compared with the AMEP412 protein which is not subjected to natural placement treatment, the AMEP412 protein reduces the fatality rate of the white whitefly by 2.73% after being naturally placed for 24 hours at 25 ℃, and the difference is not obvious. And after the AMEP412 protein is naturally placed at 25 ℃ for 48 hours, the fatality rate to the trialeurodes vaporariorum is reduced by 16.38%. Although a significant difference was achieved, the mortality rate was still 70%, so the AMEP412 protein was considered to have good stability to natural degradation. These results demonstrate that AMEP412 has stability to heat and to natural degradation, facilitates application in high temperature environments, and prolongs its duration of action.
Example 3
This example illustrates the fluorescent localization of the Bacillus subtilis AMEP412 protein in whitefly.
And (3) incubating the AMEP412 protein with fluorescein FITC, and removing FITC which is not combined with the AMEP412 protein according to the molecular volume difference by molecular sieve chromatography to obtain the FITC-labeled AMEP412 protein. FITC-labeled AMEP412 protein was subsequently fed to adult whitefly. Dead whitefly adults were washed with distilled water and then fluorescence microscopic observation was performed by exciting green fluorescence at a wavelength of 495nm using an Olympus BX60 fluorescence microscope. The results show (fig. 3) that the green fluorescence is concentrated in the intestinal tract of the adult whitefly, indicating that the AMEP412 protein can be taken up by the adult whitefly into the body and localized in the intestinal tract. This result suggests that our AMEP412 is in the gut where it exerts its insecticidal effect.
Example 4
This example illustrates the effect of Bacillus subtilis AMEP412 protein on trypsin activity.
Some insecticidal proteins act by acting as protease inhibitors, by inhibiting the activity of serine proteases (e.g., trypsin) in the insect gut, which render the insect indigestible and the ingested food lethal. To determine whether the Bacillus subtilis AMEP412 protein acts as a protease inhibitor to achieve pesticidal effects, this example examined the effect of AMEP412 protein on trypsin activity.
The trypsin activity assay was performed on the basis of the detection of the product of the trypsin catalyzed production of the substrate N- α -benzoyl-DL-arginine-p-nitroanilide (BAPNA). BAPNA has a specific absorption at 405 nm. The effect on trypsin activity can be determined by measuring the change in absorbance. 0.2ml trypsin solution (0.1mg/ml), 0.2ml AMEP412 protein (0.1mg/ml) and 1.6ml buffer (0.2M Tris HCl, pH7.8) were incubated in the reaction cup for 3 minutes. The enzymatic reaction was started by adding 1ml of substrate (1mg/ml BAPNA). The solution was then mixed immediately and the increase in absorbance at 405nm was recorded every minute for 5 minutes. No AMEP protein was added as a control. Each treatment was repeated three times. The inhibition rate was calculated as follows: inhibition (%) - (treatment Δ a405 nm/min by AMEP 412-control Δ a405 nm/min:): 100/control Δ a405 nm/min.
The results show (table 2) that the inhibition rate of the AMEP412 protein on trypsin is 2.97%, and there is no significant difference. The amap 412 protein is shown to have no inhibitory effect on trypsin activity, suggesting that the insecticidal activity of the AMEP412 is not a mechanism of action of protease inhibitors.
TABLE 2 Effect of AMEP412 protein on Trypsin Activity
Example 5
This example illustrates the control effect of Bacillus subtilis AMEP412 protein on whitefly in the greenhouse.
Tomato plants were grown in a greenhouse to a height of about 50cm, and three tomatoes were treated as one, covered with a gauze, and inoculated with at least 200 adult whiteflies of 1-2 days old per gauze. The purified exciton AMEP412 protein sample was diluted to 100. mu.g/ml for spray treatment, with buffer as control, and each treatment was repeated 3 times. And (5) counting the mortality of the trialeurodes vaporariorum 2 days after spraying.
The results show (table 3) that the mortality of trialeurodes vaporariorum was 56.2% with a very significant difference (α ═ 1%) after two days of spraying of 100 μ g/ml of AMEP412 protein. Although the mortality rate is reduced as compared with the feeding experiment, it is desirable that the inhibition rate is 50% or more in consideration of the influence of various factors in the actual environment. Therefore, the Bacillus subtilis AMEP412 protein has a good prevention and control effect on the trialeurodes vaporariorum in a greenhouse.
TABLE 3 Effect of AMEP412 protein on controlling Bemisia alba in greenhouse
The invention discovers the insecticidal activity of the bacillus subtilis protein AMEP412 on whitefly imagoes, further confirms the stability of the protein on heat treatment and natural degradation, detects the positioning of the protein in whitefly bodies, and confirms the cracking effect of the protein on insect cells. Furthermore, the invention detects the control effect of the protein on the trialeurodes vaporariorum in a greenhouse, and accumulates new materials and experiences for biological control work.
Sequence listing
<110> university of eight agricultural reclamation of Heilongjiang
<120> insecticidal activity of AMEP412 protein on trialeurodes vaporariorum and application thereof in control of trialeurodes vaporariorum
<141> 2019-12-02
<160> 1
<170> SIPOSequenceListing 1.0
<210> 1
<211> 76
<212> PRT
<213> Bacillus subtilis
<400> 1
Met Phe Gly Pro Ile Leu Lys Ala Leu Lys Ala Leu Val Ser Lys Val
1 5 10 15
Pro Trp Gly Lys Val Ala Ser Phe Leu Lys Trp Ala Gly Asn Leu Ala
20 25 30
Ala Ala Ala Ala Lys Tyr Ser Tyr Thr Ser Gly Lys Lys Ile Leu Ala
35 40 45
Tyr Ile Gln Lys His Pro Gly Lys Ile Val Asp Trp Phe Leu Lys Gly
50 55 60
Tyr Ser Val Tyr Asp Val Ile Lys Met Ile Leu Gly
65 70 75
Claims (6)
1. An application of Bacillus subtilis protein AMEP412 with an amino acid sequence shown in SEQ ID NO. 1 in controlling trialeurodes vaporariorum.
2. Use according to claim 1, characterized in that: after the AMEP412 protein is fed to whitefly adults, the protein concentration required for reaching half lethal dose is 15.57 mug/ml, and the protein concentration required for reaching 90% mortality is 63.27 mug/ml.
3. Use according to claim 1, characterized in that: the AMEP412 protein can be ingested into the body by whitefly adults and is concentrated in the intestinal tract.
4. Use according to claim 1, characterized in that: the AMEP412 protein has heat-resistant stability.
5. Use according to claim 1, characterized in that: the AMEP412 protein has stability to natural degradation.
6. Use according to claim 1, characterized in that: the spraying concentration of the AMEP412 protein in a greenhouse is 100 mug/ml, and the mortality rate of the trialeurodes vaporariorum after 2 days is 56%.
Priority Applications (2)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201911212945.9A CN110754471B (en) | 2019-12-02 | 2019-12-02 | Insecticidal activity of AMEP412 protein on trialeurodes vaporariorum and application of insecticidal activity to trialeurodes vaporariorum |
| NL2026309A NL2026309B1 (en) | 2019-12-02 | 2020-08-20 | Insecticidal Activity of AMEP412 Protein Against Whitefly And Application Thereof in Preventing and Controlling Whitefly. |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201911212945.9A CN110754471B (en) | 2019-12-02 | 2019-12-02 | Insecticidal activity of AMEP412 protein on trialeurodes vaporariorum and application of insecticidal activity to trialeurodes vaporariorum |
Publications (2)
| Publication Number | Publication Date |
|---|---|
| CN110754471A CN110754471A (en) | 2020-02-07 |
| CN110754471B true CN110754471B (en) | 2021-04-13 |
Family
ID=69340485
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN201911212945.9A Active CN110754471B (en) | 2019-12-02 | 2019-12-02 | Insecticidal activity of AMEP412 protein on trialeurodes vaporariorum and application of insecticidal activity to trialeurodes vaporariorum |
Country Status (2)
| Country | Link |
|---|---|
| CN (1) | CN110754471B (en) |
| NL (1) | NL2026309B1 (en) |
Families Citing this family (5)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN111574598B (en) * | 2020-06-05 | 2023-02-03 | 黑龙江八一农垦大学 | Method for improving AMEP412 protein yield and application of method in plant immunity stimulation |
| CN113527439B (en) * | 2021-07-05 | 2022-04-12 | 湖北省生物农药工程研究中心 | Tetranychus-killing protein BVP8 and application thereof |
| CN113788882B (en) * | 2021-08-12 | 2022-04-26 | 黑龙江八一农垦大学 | Application of bacillus subtilis AMEP412 protein in inhibiting tumor cell proliferation |
| CN114831216B (en) * | 2022-05-30 | 2023-07-21 | 黑龙江权晟生物科技有限公司 | Application of bacillus subtilis AMEP412 protein in regulating animal immunity |
| CN115651072A (en) * | 2022-06-16 | 2023-01-31 | 黑龙江权晟生物科技有限公司 | Application of bacillus subtilis AMEP412 protein in regulation of periphyton microbial community structure |
Citations (4)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN1119877A (en) * | 1993-03-25 | 1996-04-03 | 希巴-盖吉股份公司 | Novel pesticidal proteins and strains |
| CN102191194A (en) * | 2011-03-17 | 2011-09-21 | 四川师范大学 | Bacillus subtilis and antibacterial protein thereof |
| MX2016017087A (en) * | 2016-12-19 | 2018-06-18 | Biokrone S A De C V | A biofungicide composed by a consortium of three strains of subtitlis lactobacilli for the control of fungi, nematodes, and phytopathogens insects. |
| CN108929866A (en) * | 2018-06-11 | 2018-12-04 | 黑龙江八农垦大学 | The new function and its antibacterial peptide of bacillus subtilis GGT protein degradation product are identified |
-
2019
- 2019-12-02 CN CN201911212945.9A patent/CN110754471B/en active Active
-
2020
- 2020-08-20 NL NL2026309A patent/NL2026309B1/en not_active IP Right Cessation
Patent Citations (4)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN1119877A (en) * | 1993-03-25 | 1996-04-03 | 希巴-盖吉股份公司 | Novel pesticidal proteins and strains |
| CN102191194A (en) * | 2011-03-17 | 2011-09-21 | 四川师范大学 | Bacillus subtilis and antibacterial protein thereof |
| MX2016017087A (en) * | 2016-12-19 | 2018-06-18 | Biokrone S A De C V | A biofungicide composed by a consortium of three strains of subtitlis lactobacilli for the control of fungi, nematodes, and phytopathogens insects. |
| CN108929866A (en) * | 2018-06-11 | 2018-12-04 | 黑龙江八农垦大学 | The new function and its antibacterial peptide of bacillus subtilis GGT protein degradation product are identified |
Non-Patent Citations (7)
| Title |
|---|
| AMEP 蛋白在不同芽孢杆菌中的表达量及活性差异分析;郭世伦等;《安徽农学通报》;20201231;第26卷(第20期);23-25 * |
| Effect of the protein elicitor AMEP412 from Bacillus subtilis artificially fed to adults of the whitefly, Bemisia tabaci (Genn.) (Hemiptera: Aleyrodidae);Quan Liu et al;《Egyptian Journal of Biological Pest Control》;20200116(第30期);3 * |
| GenBank：WP_017418614.1;无;《NCBI》;20170504 * |
| Isolation and identification of a novel protein elicitor from a Bacillus subtilis strain BU412;Yongrui Shen et al;《AMB Expr》;20190727(第9期);117 * |
| The antimicrobial activity of protein elicitor AMEP412 against Streptomyces scabiei;Quan Liu et al;《World Journal of Microbiology and Biotechnology》;20200107;第36卷(第18期);18 * |
| 抗菌蛋白AMEP412的鉴定及其在防治马铃薯疮痂病上的应用;申永瑞;《中国优秀硕士学位论文全文数据库 农业科技辑》;20201115(第11期);D046-16 * |
| 枯草芽孢杆菌的研究与应用;李明等;《东北农业大学学报》;20090930;第40卷(第9期);111-114 * |
Also Published As
| Publication number | Publication date |
|---|---|
| CN110754471A (en) | 2020-02-07 |
| NL2026309A (en) | 2021-07-30 |
| NL2026309B1 (en) | 2022-06-14 |
Similar Documents
| Publication | Publication Date | Title |
|---|---|---|
| CN110754471B (en) | Insecticidal activity of AMEP412 protein on trialeurodes vaporariorum and application of insecticidal activity to trialeurodes vaporariorum | |
| US9205115B2 (en) | Bacillus isolates and methods of their use to protect against plant pathogens and virus transmission | |
| RU2311767C2 (en) | Method for combating of agrotis pest earworm | |
| SK6562001A3 (en) | Compositions and methods for controlling plant pests | |
| Narayanasamy et al. | Mechanisms of action of fungal biological control agents | |
| KR20140062099A (en) | Insecticidal lipid agents isolated from entomopathogenic fungi and uses thereof | |
| US20220104487A1 (en) | Pseudomonas strains and their metabolites to control plant diseases | |
| Liu et al. | Fungal secondary metabolites in biological control of crop pests | |
| KR20140017626A (en) | Insecticidal agents and uses thereof | |
| CN107427011A (en) | Prevent in crops and ornamental plant, the method for the infection preferably in wine-growing and xylophyta | |
| KR102126582B1 (en) | Microbial agent for simultaneous control of both Colletotrichum acutatum and Thrips spp. using Isaria javanica FT333 and its culture media | |
| Kesho | Microbial bio-pesticides and their use in integrated pest management | |
| US20200128833A1 (en) | Use of an extract of part of a rocket plant for stimulating the defenses of plants and trees and associated composition and method | |
| KR100319135B1 (en) | Microbiological preparation inhibiting plant diseases | |
| Ashrafi et al. | Rosemary wilting disease and its management by soil solarization technique in Iran | |
| Mohamedova | Field evaluation of three biopesticides for control of the raspberry cane midge, Resseliella theobaldi (Barnes) in Bulgaria | |
| Narayan et al. | Plant pathogenesis and disease control | |
| Manjunatha et al. | Disease Management of Faba Beans | |
| RU2812480C1 (en) | Bacillus thuringiensis var. darmstadiensis 109/7 strain as polyfunctional plant protector and stimulation of their growth | |
| Sarkar et al. | Current Status of Tomato (Solanum Lycopersicum L.) Diseases and Their Management | |
| Jain et al. | Identification of Etiological Agent of Telya Disease of Pomegranate, its Pathogenesis and Control using Integrated Management Approach | |
| Piencek et al. | The evaluation of infection caused by different fungi species of highbush blueberry plants grown on selected commercial plantations located in central Poland | |
| Luján et al. | Fungal extracts as biocontrol of growth, biofilm formation, and motility of xanthomonas citri subsp. citri | |
| CA3197854A1 (en) | Pseudomonas strains and their metabolites to control plant diseases | |
| DI DOMENICO | Control of Philaenus spumarius (Linnaeus, 1758) using beneficial microorganisms and their metabolites in preventing the transmission of Xylella fastidiosa Wells et al.(1987) |
Legal Events
| Date | Code | Title | Description |
|---|---|---|---|
| PB01 | Publication | ||
| PB01 | Publication | ||
| SE01 | Entry into force of request for substantive examination | ||
| SE01 | Entry into force of request for substantive examination | ||
| GR01 | Patent grant | ||
| GR01 | Patent grant |