CN110746713B - 一种用于检测次氯酸根比色-荧光纤维膜及其制备方法 - Google Patents

一种用于检测次氯酸根比色-荧光纤维膜及其制备方法 Download PDF

Info

Publication number
CN110746713B
CN110746713B CN201911093800.1A CN201911093800A CN110746713B CN 110746713 B CN110746713 B CN 110746713B CN 201911093800 A CN201911093800 A CN 201911093800A CN 110746713 B CN110746713 B CN 110746713B
Authority
CN
China
Prior art keywords
colorimetric
fiber membrane
hypochlorite
polyhydroxyethyl methacrylate
mixed material
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Active
Application number
CN201911093800.1A
Other languages
English (en)
Other versions
CN110746713A (zh
Inventor
蔡珍珍
窦新存
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
Xinjiang Technical Institute of Physics and Chemistry of CAS
Original Assignee
Xinjiang Technical Institute of Physics and Chemistry of CAS
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Xinjiang Technical Institute of Physics and Chemistry of CAS filed Critical Xinjiang Technical Institute of Physics and Chemistry of CAS
Priority to CN201911093800.1A priority Critical patent/CN110746713B/zh
Publication of CN110746713A publication Critical patent/CN110746713A/zh
Application granted granted Critical
Publication of CN110746713B publication Critical patent/CN110746713B/zh
Active legal-status Critical Current
Anticipated expiration legal-status Critical

Links

Images

Classifications

    • C—CHEMISTRY; METALLURGY
    • C08—ORGANIC MACROMOLECULAR COMPOUNDS; THEIR PREPARATION OR CHEMICAL WORKING-UP; COMPOSITIONS BASED THEREON
    • C08J—WORKING-UP; GENERAL PROCESSES OF COMPOUNDING; AFTER-TREATMENT NOT COVERED BY SUBCLASSES C08B, C08C, C08F, C08G or C08H
    • C08J5/00—Manufacture of articles or shaped materials containing macromolecular substances
    • C08J5/18—Manufacture of films or sheets
    • C—CHEMISTRY; METALLURGY
    • C08—ORGANIC MACROMOLECULAR COMPOUNDS; THEIR PREPARATION OR CHEMICAL WORKING-UP; COMPOSITIONS BASED THEREON
    • C08F—MACROMOLECULAR COMPOUNDS OBTAINED BY REACTIONS ONLY INVOLVING CARBON-TO-CARBON UNSATURATED BONDS
    • C08F220/00—Copolymers of compounds having one or more unsaturated aliphatic radicals, each having only one carbon-to-carbon double bond, and only one being terminated by only one carboxyl radical or a salt, anhydride ester, amide, imide or nitrile thereof
    • C08F220/02—Monocarboxylic acids having less than ten carbon atoms; Derivatives thereof
    • C08F220/10—Esters
    • C08F220/20—Esters of polyhydric alcohols or phenols, e.g. 2-hydroxyethyl (meth)acrylate or glycerol mono-(meth)acrylate
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N21/00—Investigating or analysing materials by the use of optical means, i.e. using sub-millimetre waves, infrared, visible or ultraviolet light
    • G01N21/62—Systems in which the material investigated is excited whereby it emits light or causes a change in wavelength of the incident light
    • G01N21/63—Systems in which the material investigated is excited whereby it emits light or causes a change in wavelength of the incident light optically excited
    • G01N21/64—Fluorescence; Phosphorescence
    • G01N21/6428—Measuring fluorescence of fluorescent products of reactions or of fluorochrome labelled reactive substances, e.g. measuring quenching effects, using measuring "optrodes"
    • G01N21/643—Measuring fluorescence of fluorescent products of reactions or of fluorochrome labelled reactive substances, e.g. measuring quenching effects, using measuring "optrodes" non-biological material
    • C—CHEMISTRY; METALLURGY
    • C08—ORGANIC MACROMOLECULAR COMPOUNDS; THEIR PREPARATION OR CHEMICAL WORKING-UP; COMPOSITIONS BASED THEREON
    • C08J—WORKING-UP; GENERAL PROCESSES OF COMPOUNDING; AFTER-TREATMENT NOT COVERED BY SUBCLASSES C08B, C08C, C08F, C08G or C08H
    • C08J2325/00—Characterised by the use of homopolymers or copolymers of compounds having one or more unsaturated aliphatic radicals, each having only one carbon-to-carbon double bond, and at least one being terminated by an aromatic carbocyclic ring; Derivatives of such polymers
    • C08J2325/02—Homopolymers or copolymers of hydrocarbons
    • C08J2325/04—Homopolymers or copolymers of styrene
    • C08J2325/06—Polystyrene
    • C—CHEMISTRY; METALLURGY
    • C08—ORGANIC MACROMOLECULAR COMPOUNDS; THEIR PREPARATION OR CHEMICAL WORKING-UP; COMPOSITIONS BASED THEREON
    • C08J—WORKING-UP; GENERAL PROCESSES OF COMPOUNDING; AFTER-TREATMENT NOT COVERED BY SUBCLASSES C08B, C08C, C08F, C08G or C08H
    • C08J2433/00—Characterised by the use of homopolymers or copolymers of compounds having one or more unsaturated aliphatic radicals, each having only one carbon-to-carbon double bond, and only one being terminated by only one carboxyl radical, or of salts, anhydrides, esters, amides, imides, or nitriles thereof; Derivatives of such polymers
    • C08J2433/04—Characterised by the use of homopolymers or copolymers of compounds having one or more unsaturated aliphatic radicals, each having only one carbon-to-carbon double bond, and only one being terminated by only one carboxyl radical, or of salts, anhydrides, esters, amides, imides, or nitriles thereof; Derivatives of such polymers esters
    • C08J2433/14—Characterised by the use of homopolymers or copolymers of compounds having one or more unsaturated aliphatic radicals, each having only one carbon-to-carbon double bond, and only one being terminated by only one carboxyl radical, or of salts, anhydrides, esters, amides, imides, or nitriles thereof; Derivatives of such polymers esters of esters containing halogen, nitrogen, sulfur, or oxygen atoms in addition to the carboxy oxygen
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N21/00—Investigating or analysing materials by the use of optical means, i.e. using sub-millimetre waves, infrared, visible or ultraviolet light
    • G01N21/62—Systems in which the material investigated is excited whereby it emits light or causes a change in wavelength of the incident light
    • G01N21/63—Systems in which the material investigated is excited whereby it emits light or causes a change in wavelength of the incident light optically excited
    • G01N21/64—Fluorescence; Phosphorescence
    • G01N21/6428—Measuring fluorescence of fluorescent products of reactions or of fluorochrome labelled reactive substances, e.g. measuring quenching effects, using measuring "optrodes"
    • G01N2021/6432—Quenching

Landscapes

  • Chemical & Material Sciences (AREA)
  • Health & Medical Sciences (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • Physics & Mathematics (AREA)
  • Medicinal Chemistry (AREA)
  • Polymers & Plastics (AREA)
  • Organic Chemistry (AREA)
  • Engineering & Computer Science (AREA)
  • Manufacturing & Machinery (AREA)
  • Immunology (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Molecular Biology (AREA)
  • Materials Engineering (AREA)
  • Optics & Photonics (AREA)
  • Nuclear Medicine, Radiotherapy & Molecular Imaging (AREA)
  • Analytical Chemistry (AREA)
  • Biochemistry (AREA)
  • General Health & Medical Sciences (AREA)
  • General Physics & Mathematics (AREA)
  • Pathology (AREA)
  • Investigating Or Analysing Materials By The Use Of Chemical Reactions (AREA)
  • Investigating Or Analyzing Non-Biological Materials By The Use Of Chemical Means (AREA)

Abstract

本发明提供一种用于检测次氯酸根比色‑荧光纤维膜及制备方法，该荧光纤维膜是将含双键比色‑荧光探针分子（（E）‑（（4‑（2‑（4‑氰基‑5‑（二氰基亚甲基）‑2,2‑二甲基‑2,5‑二氢呋喃‑3‑基）乙烯基）苯基）氮烷二基）双（乙烷‑2，1‑二基）二丙烯酸酯）与甲基丙烯酸羟乙酯采用自由基聚合得到改性聚甲基丙烯酸羟乙酯，再将改性聚甲基丙烯酸羟乙酯与聚苯乙烯经研磨制成，所述的检测次氯酸根比色‑荧光纤维膜，其荧光发射峰位在632 nm，紫外吸收峰位在544 nm，用于对次氯酸根的检测时，表现出明显的荧光猝灭和褪色响应，响应速度快，实现对环境中水样及非制式爆炸物原料中的次氯酸根的现场、快速、便捷，可视化检测。

Description

一种用于检测次氯酸根比色-荧光纤维膜及其制备方法
技术领域
本发明涉及静电纺丝技术及环境和非制式爆炸物检测领域，提供了一种用于制备检测次氯酸根比色-荧光纤维膜及其制备方法。
背景技术
静电纺丝法是一种简单、有效制备连续纳米纤维膜的方法，所纺纤维具有直径小、比表面积大、孔隙率高和均一性好等优点，被广泛应用于过滤、防护纺织品、药物输送、组织工程、电子和光子设备、传感器和催化等领域。2007年，李广涛教授(Journal of MaterialsChemistry 2007,17,2730-2736)首次将电纺技术引入到TNT传感领域，可检测到10ppb级的气态TNT分子。Kumar(Technologies for Homeland Security,IEEE Conference on 2008,390-394)等开展了静电纺丝制备荧光高分子纳米纤维膜的工作并将其用于爆炸物检测。自此开启了静电纺丝纤维膜用于爆炸物检测领域。
次氯酸根作为一种非常重要的活性氧，在生物体和饮用水中起到至关重要的作用。但若生物体内积累过多，会引起各种疾病。除此之外，据文献调研发现，目前人们对于非制式爆炸物中氯酸盐、高氯酸盐、硝酸盐和亚硝酸盐等的检测进行了大量研究，而次氯酸盐作为非制式爆炸物中的一种重要原料，还远远没能引起足够的重视。次氯酸钙和刹车油按一定比例混合即可作为爆炸物使用。此外，超市中就能买到的低盐(氯化钾)和消毒水(次氯酸钠)混合在一起就能轻易制备出非制式爆炸物中的氯酸钾。因此，对环境及非制式爆炸物中次氯酸盐的检测是非常有必要的。
目前，检测次氯酸根的方法主要是溶液法，配合分光光度、化学发光等大型仪器。这种分析方法不仅需要预先配制好检测液还需要昂贵的仪器及专业的操作人员。因此，亟需开发可便捷、低成本、快速检测次氯酸根的方法。
本发明从对次氯酸根具有较好检测性能的比色-荧光探针分子((E)-((4-(2-(4-氰基-5-(二氰基亚甲基)-2,2-二甲基-2,5-二氢呋喃-3-基)乙烯基)苯基)氮烷二基)双(乙烷-2，1-二基)二丙烯酸酯)(CN201910957006.0)入手，采用自由基聚合与甲基丙烯酸羟乙酯共聚制备改性聚甲基丙烯酸羟乙酯，再与聚苯乙烯混合制备混合物料后溶解，采用静电纺丝技术制备比色-荧光纤维膜，从而实现对次氯酸根的便捷、快速检测。
发明内容
本发明目的在于，针对现有技术中存在的不足，提供一种用于检测次氯酸根比色-荧光纤维膜及其制备方法，该荧光纤维膜是将含双键比色-荧光探针分子((E)-((4-(2-(4-氰基-5-(二氰基亚甲基)-2,2-二甲基-2,5-二氢呋喃-3-基)乙烯基)苯基)氮烷二基)双(乙烷-2，1-二基)二丙烯酸酯)与甲基丙烯酸羟乙酯采用自由基聚合得到改性聚甲基丙烯酸羟乙酯，再将改性聚甲基丙烯酸羟乙酯与聚苯乙烯经研磨制成，所述的检测次氯酸根比色-荧光纤维膜，其荧光发射峰位在632nm，紫外吸收峰位在544nm，用于对次氯酸根的检测时，表现出明显的荧光猝灭和褪色响应，响应速度快，实现对环境中水样及非制式爆炸物原料中的次氯酸根的现场、快速、便捷，可视化检测。
本发明所述的一种用于检测次氯酸根比色-荧光纤维膜，该荧光纤维膜是由改性聚甲基丙烯酸羟乙酯和聚苯乙烯制成，所述改性聚甲基丙烯酸羟乙酯的化学结构式(1)为：
Figure BDA0002267673610000021
其中：改性聚甲基丙烯酸羟乙酯占混合物料的5-20wt％，聚苯乙烯占混合物料的80-95wt％；
改性聚甲基丙烯酸羟乙酯是由比色-荧光探针分子含双键((E)-((4-(2-(4-氰基-5-(二氰基亚甲基)-2,2-二甲基-2,5-二氢呋喃-3-基)乙烯基)苯基)氮烷二基)双(乙烷-2，1-二基)二丙烯酸酯)与甲基丙烯酸羟乙酯按摩尔比1:100-1:1000采用自由基共聚制备。
所述一种用于检测次氯酸根比色-荧光纤维膜的制备方法，按下列步骤进行：
制备改性聚甲基丙烯酸羟乙酯：
a、按摩尔比1:100-1:1000，将比色-荧光探针分子含双键((E)-((4-(2-(4-氰基-5-(二氰基亚甲基)-2,2-二甲基-2,5-二氢呋喃-3-基)乙烯基)苯基)氮烷二基)双(乙烷-2，1-二基)二丙烯酸酯)与甲基丙烯酸羟乙酯溶于10倍摩尔量的四氢呋喃中，通氮气20min后，加入0.02倍摩尔量的引发剂偶氮二异丁腈，升温至70℃，回流反应12h；冷却至室温，再将反应液于石油醚中沉积，抽滤、洗涤、干燥，即得改性聚甲基丙烯酸羟乙酯；
制备检测次氯酸根比色-荧光纤维膜：
b、将步骤a得到的改性聚甲基丙烯酸羟乙酯与聚苯乙烯置于研钵中研磨至粉末，并混合均匀，得混合物料，其中改性聚甲基丙烯酸羟乙酯占混合物料的5-20wt％，聚苯乙烯占混合物料的80-95wt％；
c、将步骤b得到的含量为10wt％-20wt％混合物料溶于体积比为1:10的四氢呋喃和二甲基亚砜混合溶液中，搅拌至溶解均匀后，过滤料液、消泡后从圆形纤维喷孔中挤出料液，挤出电压为20KV，挤出速率为0.06mL/h，挤出时间为2h，即得检测次氯酸根比色-荧光纤维膜。
本发明所述的一种用于检测次氯酸根比色-荧光纤维膜及其制备方法，该方法的工艺流程为：
Figure BDA0002267673610000031
与现有技术相比，本发明的有益效果是：
本发明所述的一种用于检测次氯酸根比色-荧光纤维膜，可实现对环境中水样及非制式爆炸物原料中的次氯酸根的现场、快速、可视化检测，在632nm处出现荧光猝灭，在544nm处出现褪色变化；无需对待测物进行前处理，操作简单，便捷，高效。具有检测限低、灵敏度高的优点，可实现低成本、快速检测次氯酸根的目的。
附图说明
图1为本发明检测次氯酸根比色-荧光纤维膜扫描电镜图。
具体实施方式
下面通过具体实施例对本发明做进一步的说明，但发明不限制于这些实施例。
实施例1
制备改性聚甲基丙烯酸羟乙酯：
a、将1mmol比色-荧光探针分子含双键((E)-((4-(2-(4-氰基-5-(二氰基亚甲基)-2,2-二甲基-2,5-二氢呋喃-3-基)乙烯基)苯基)氮烷二基)双(乙烷-2，1-二基)二丙烯酸酯)与100mmol甲基丙烯酸羟乙酯溶于1010mmol四氢呋喃中，通氮气20min后，加入2.02mmol引发剂偶氮二异丁腈，升温至70℃，回流反应12h，冷却至室温，再将反应液于5000mL石油醚中沉积，抽滤、洗涤、干燥，即得改性聚甲基丙烯酸羟乙酯80mmol，产率79.2％；
制备检测次氯酸根比色-荧光纤维膜：
b、将步骤a得到的改性聚甲基丙烯酸羟乙酯0.5g与聚苯乙烯9.5g置于研钵中研磨至粉末，并混合均匀，得混合物料10g；
c、将步骤b得到的混合物料1g溶于体积比为1:10的四氢呋喃和二甲基亚砜混合溶液9g中，搅拌至溶解均匀后，过滤料液、消泡后从圆形纤维喷孔中挤出料液，挤出电压为20KV，挤出速率为0.06mL/h，挤出时间为2h，即得检测次氯酸根比色-荧光纤维膜，扫描电镜图如图1所示。
实施例2
制备改性聚甲基丙烯酸羟乙酯：
a、将0.1mmol比色-荧光探针分子含双键((E)-((4-(2-(4-氰基-5-(二氰基亚甲基)-2,2-二甲基-2,5-二氢呋喃-3-基)乙烯基)苯基)氮烷二基)双(乙烷-2，1-二基)二丙烯酸酯)与100mmol甲基丙烯酸羟乙酯溶于1001mmol四氢呋喃中，通氮气20min后，加入2.002mmol引发剂偶氮二异丁腈，升温至70℃，回流反应12h，冷却至室温，反应液于5000mL石油醚中沉积，抽滤、洗涤、干燥，即得改性聚甲基丙烯酸羟乙酯76mmol，产率75.9％；
制备检测次氯酸根比色-荧光纤维膜：
b、将步骤a得到的改性聚甲基丙烯酸羟乙酯2.0g与聚苯乙烯8.0g置于研钵中研磨至粉末，并混合均匀，得混合物料10g；
c、将步骤b得到的混合物料2g溶于体积比为1:10的四氢呋喃和二甲基亚砜混合溶液8g中，搅拌至溶解均匀后，过滤料液、消泡后从圆形纤维喷孔中挤出料液，挤出电压为20KV，挤出速率为0.06mL/h，挤出时间为2h，即得检测次氯酸根比色-荧光纤维膜。
实施例3
制备改性聚甲基丙烯酸羟乙酯：
a、将0.2mmol比色-荧光探针分子含双键((E)-((4-(2-(4-氰基-5-(二氰基亚甲基)-2,2-二甲基-2,5-二氢呋喃-3-基)乙烯基)苯基)氮烷二基)双(乙烷-2，1-二基)二丙烯酸酯)与100mmol甲基丙烯酸羟乙酯溶于1002mmol四氢呋喃中，通氮气20min后，加入2.004mmol引发剂偶氮二异丁腈，升温至70℃，回流反应12h，冷却至室温，反应液于5000mL石油醚中沉积，抽滤、洗涤、干燥，即得改性聚甲基丙烯酸羟乙酯77mmol，产率77.2％；
制备检测次氯酸根比色-荧光纤维膜：
b、将步骤a得到的改性聚甲基丙烯酸羟乙酯1.25g与聚苯乙烯8.75g置于研钵中研磨至粉末，并混合均匀，得混合物料10g；
c、将步骤b得到的混合物料1.5g溶于体积比为1:10的四氢呋喃和二甲基亚砜混合溶液中，搅拌至溶解均匀后，过滤料液、消泡后从圆形纤维喷孔中挤出料液，挤出电压为20KV，挤出速率为0.06mL/h，挤出时间为2h，即得检测次氯酸根比色-荧光纤维膜。
实施例4
取实施例1-3任意一种检测次氯酸根比色-荧光纤维膜，滴加5uM的次氯酸盐溶液5uL，纤维膜的颜色由紫色褪为无色544nm处吸收峰消失，荧光猝灭632nm处荧光发射峰消失。

Claims (2)

1.一种用于检测次氯酸根比色-荧光纤维膜，其特征在于，该荧光纤维膜是由改性聚甲基丙烯酸羟乙酯和聚苯乙烯制成，所述改性聚甲基丙烯酸羟乙酯的化学结构式(1)为：
Figure FDA0003339969590000011
其中：改性聚甲基丙烯酸羟乙酯占混合物料的5-20wt％，聚苯乙烯占混合物料的80-95wt％；
改性聚甲基丙烯酸羟乙酯是由比色-荧光探针分子含双键((E)-((4-(2-(4-氰基-5-(二氰基亚甲基)-2,2-二甲基-2,5-二氢呋喃-3-基)乙烯基)苯基)氮烷二基)双(乙烷-2，1-二基)二丙烯酸酯)与甲基丙烯酸羟乙酯按摩尔比1:100-1:1000采用自由基共聚制备。
2.根据权利要求1所述的一种用于检测次氯酸根比色-荧光纤维膜的制备方法，其特征在于按下列步骤进行：
制备改性聚甲基丙烯酸羟乙酯：
a、按摩尔比1:100-1:1000，将比色-荧光探针分子含双键((E)-((4-(2-(4-氰基-5-(二氰基亚甲基)-2,2-二甲基-2,5-二氢呋喃-3-基)乙烯基)苯基)氮烷二基)双(乙烷-2，1-二基)二丙烯酸酯)与甲基丙烯酸羟乙酯溶于10倍摩尔量的四氢呋喃中，通氮气20min后，加入0.02倍摩尔量的引发剂偶氮二异丁腈，升温至70℃，回流反应12h；冷却至室温，再将反应液于石油醚中沉积，抽滤、洗涤、干燥，即得改性聚甲基丙烯酸羟乙酯；
制备检测次氯酸根比色-荧光纤维膜：
b、将步骤a得到的改性聚甲基丙烯酸羟乙酯与聚苯乙烯置于研钵中研磨至粉末，并混合均匀，得混合物料，其中改性聚甲基丙烯酸羟乙酯占混合物料的5-20wt％，聚苯乙烯占混合物料的80-95wt％；
c、将步骤b得到的混合物料的10wt％-20wt％溶于体积比为1:10的四氢呋喃和二甲基亚砜混合溶液中，搅拌至溶解均匀后，过滤料液、消泡后从圆形纤维喷孔中挤出料液，挤出电压为20KV，挤出速率为0.06mL/h，挤出时间为2h，即得检测次氯酸根比色-荧光纤维膜。
CN201911093800.1A 2019-11-11 2019-11-11 一种用于检测次氯酸根比色-荧光纤维膜及其制备方法 Active CN110746713B (zh)

Priority Applications (1)

Application Number Priority Date Filing Date Title
CN201911093800.1A CN110746713B (zh) 2019-11-11 2019-11-11 一种用于检测次氯酸根比色-荧光纤维膜及其制备方法

Applications Claiming Priority (1)

Application Number Priority Date Filing Date Title
CN201911093800.1A CN110746713B (zh) 2019-11-11 2019-11-11 一种用于检测次氯酸根比色-荧光纤维膜及其制备方法

Publications (2)

Publication Number Publication Date
CN110746713A CN110746713A (zh) 2020-02-04
CN110746713B true CN110746713B (zh) 2022-02-11

Family

ID=69282851

Family Applications (1)

Application Number Title Priority Date Filing Date
CN201911093800.1A Active CN110746713B (zh) 2019-11-11 2019-11-11 一种用于检测次氯酸根比色-荧光纤维膜及其制备方法

Country Status (1)

Country Link
CN (1) CN110746713B (zh)

Families Citing this family (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN112321549B (zh) * 2020-10-28 2022-07-08 武汉工程大学 一种远红光溶酶体荧光探针及其制备方法和应用

Family Cites Families (3)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US8143069B2 (en) * 2006-03-03 2012-03-27 The University Of Tokyo Fluorescent probe and method of measuring hypochlorite ion
US20160175634A1 (en) * 2014-10-14 2016-06-23 Regents Of The University Of Minnesota Compositions including matrix and biomaterial, uses thereof and methods of using the same
CN108774639B (zh) * 2018-05-31 2023-05-30 澳門帝傑數碼基因有限公司 一种定向聚合的荧光探针pcr

Also Published As

Publication number Publication date
CN110746713A (zh) 2020-02-04

Similar Documents

Publication Publication Date Title
Li et al. A novel fluorescence ratiometric pH sensor based on covalently immobilized piperazinyl-1, 8-napthalimide and benzothioxanthene
CN103214615B (zh) 一种稀土掺杂型荧光印迹聚合物的制备方法
CN110387137B (zh) 一种吲哚半花菁结构的水溶性染料及其合成方法
CN104017567B (zh) 一种含罗丹明内酰胺基团的高分子荧光探针在检测h+上的应用
DE102020132480B3 (de) Molekular geprägte fluoreszierende Polymere für einen direkten Nachweis von Glyphosat, seinen Abbauprodukten und Metaboliten
CN109627464B (zh) 一种荧光探针聚合物水凝胶及其制备方法
CN110746713B (zh) 一种用于检测次氯酸根比色-荧光纤维膜及其制备方法
CN105801479B (zh) 一种双光子黏度荧光探针及其制备方法和用途
Nguyen et al. Novel coumarin-based pH sensitive fluorescent probes for the highly alkaline pH region
CN104403051B (zh) 一种荧光选择识别三氟氯氰菊酯的分子印迹材料制备方法
Wei et al. Molecularly imprinted CsPbBr 3 quantum dot-based fluorescent sensor for trace tetracycline detection in aqueous environments
CN102731457A (zh) 一种水溶性荧光示踪聚合物及其制备方法
CN103497273A (zh) 一种水分散性多色荧光聚合物纳米粒子及其制备方法
Zhong et al. Synthesis and application of fluorescent polymer micro‐and nanoparticles
CN105954242B (zh) 一种基于罗丹明衍生物的聚合物样品及其在水含量分析中的应用
CN111534299A (zh) 一种GOQDs@PDA-ir-MIP分子印迹荧光传感器及其制备方法与应用
CN112945925B (zh) 一种香豆素基探针检测高锰酸根的方法
CN110283100A (zh) 一种化合物、制备方法及作为肼荧光探针的应用
CN110627756B (zh) 一种用于检测次氯酸根的比色-荧光探针及其制备方法和用途
CN105777758B (zh) 一种银离子荧光传感器分子及其合成和应用
CN110849854B (zh) 采用BA-Eu-MOF复合材料测定Hg2+和CH3Hg+含量的方法
CN109385751B (zh) 基于双金属有机骨架的荧光纳米纤维膜的制备方法及应用
CN107629161B (zh) 一种2,4,6-三氯苯酚荧光分子印迹聚合物及其应用
Shi et al. Polymerizable oxygen probe derived from platinum-based porphyrins for oxygen sensing and pressure-sensitive paints
CN111925316B (zh) 一种基于4-氟苯乙炔基的双光子荧光极性探针及其制备方法和用途

Legal Events

Date Code Title Description
PB01 Publication
PB01 Publication
SE01 Entry into force of request for substantive examination
SE01 Entry into force of request for substantive examination
GR01 Patent grant
GR01 Patent grant