CN110742760A - Numerical control chronic intermittent type nature oxygen deficiency case - Google Patents
Numerical control chronic intermittent type nature oxygen deficiency case Download PDFInfo
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- CN110742760A CN110742760A CN201911030939.1A CN201911030939A CN110742760A CN 110742760 A CN110742760 A CN 110742760A CN 201911030939 A CN201911030939 A CN 201911030939A CN 110742760 A CN110742760 A CN 110742760A
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- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
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- A61G10/00—Treatment rooms or enclosures for medical purposes
- A61G10/02—Treatment rooms or enclosures for medical purposes with artificial climate; with means to maintain a desired pressure, e.g. for germ-free rooms
- A61G10/023—Rooms for the treatment of patients at over- or under-pressure or at a variable pressure
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Abstract
The invention relates to the technical field of animal test equipment. In particular to a transparent observable box body for generating a chronic intermittent hypoxia state by applying a numerical control technology. According to the device, the two end parts of the closed test box are respectively connected with the nitrogen conveying pipe and the exhaust pipe which are communicated with the interior of the test box, the flow of the air inlet valve and the flow of the exhaust valve are controlled through the design of a computer application program, the nitrogen conveying pipe is connected with the nitrogen conveying bottle, and nitrogen is added into the closed test box, so that air in the closed test box is exhausted from the exhaust pipe, oxygen in the box body is rapidly exhausted, the content of oxygen in the box is reduced, an experimental animal placed in the box is caused to have an intermittent anoxic state, and an animal model of chronic intermittent anoxia is established. The device can automatically simulate the state of chronic intermittent oxygen deficiency in the human body according to the experimental requirements by the oxygen content in the test box.
Description
Technical Field
The invention relates to the technical field of animal test equipment, in particular to a numerical control chronic intermittent hypoxia chamber.
Background
The main gas component in the air has an oxygen content of 19-21% at low altitude, and the balance is nitrogen, which accounts for about 80%. The chronic intermittent hypoxia is a state of obstructive apnea occurring in the sleep of human beings, and due to the muscle relaxation of the throat and the tongue body, the airway of the throat is partially blocked or completely blocked in a short time, so that the oxygen exchange effect is reduced, the oxygen content in blood is reduced, and the systemic hypoxia state is generated. The brain is most vulnerable due to the greatest demand for oxygen. The chronic hypoxia state can cause chronic hypoxia change of the brain and organs of the whole body, so that nerve cells die, and brain atrophy and other damages are generated. The chronic intermittent hypoxia box simulates the state of the chronic intermittent hypoxia in vivo by reducing the oxygen content of the surrounding environment, and the current chronic intermittent hypoxia box puts a rat or a mouse into a closed test box, so that the oxygen content in the box is reduced only by aerobic respiration of the rat or the mouse, the state of the intermittent hypoxia of an experimental animal is caused, the animal model of the chronic hypoxia is established, and the efficiency is low due to long test time.
Disclosure of Invention
The invention aims to provide a numerical control chronic intermittent hypoxia chamber, which aims to solve the problem of low efficiency caused by long test time of the current chronic intermittent hypoxia chamber in the background technology.
In order to achieve the purpose, the invention provides the following technical scheme:
the utility model provides a chronic intermittent type nature oxygen deficiency case of numerical control, is including the proof box that is cavity seal structure, the both ends of proof box be connected with respectively with inside nitrogen gas conveyer pipe and the blast pipe that is linked together of proof box, be provided with the air inlet valve on the nitrogen gas conveyer pipe, install the exhaust valve on the blast pipe, the nitrogen gas conveyer pipe is kept away from an end connection of proof box has nitrogen gas bottle, the blast pipe is kept away from an end connection of proof box has the exhaust acceleration rate case, install the fixed plate in the exhaust acceleration rate case, the fixed plate with blast pipe export cross-section is parallel, the center department of fixed plate inlays to establish and installs exhaust acceleration rate fan, the exhaust acceleration rate case is kept away from one end of blast pipe is open.
Preferably, the output port of the nitrogen conveying bottle is provided with a threaded connector, and the inner wall of one end of the nitrogen conveying bottle connected with the nitrogen conveying bottle is of an internal thread structure and is in threaded connection with the threaded connector.
Preferably, the top of the exhaust speed increasing box is provided with a lead through hole.
Preferably, the top of the test box is provided with a plurality of sealing cover mounting screw holes which are uniformly and equidistantly arranged, the top of the test box is provided with a sealing cover, and the edge of the sealing cover is provided with connecting holes which are equal in number, correspond to the sealing cover mounting screw holes in position and are adaptive to the sealing cover mounting screw holes in size.
Preferably, the sealing cover sequentially penetrates through the connecting hole and the sealing cover mounting screw hole through a fixing bolt to be connected to the top of the test box in a threaded mode.
Preferably, the bottom of the sealing cover is provided with a sealing groove matched with the top of the test box in size.
Preferably, the air inlet valve and the air outlet valve both adopt gas mass flow controllers, flow display instruments are arranged on the air inlet valve and the air outlet valve, and each flow display instrument is connected with a computer or a PLC.
Compared with the prior art, the invention has the beneficial effects that:
1. this chronic intermittent type nature oxygen deficiency case of numerical control is connected with nitrogen gas conveyer pipe and the blast pipe that is linked together with the proof box inside respectively through both ends at airtight proof box, and with nitrogen gas duct connection in nitrogen gas conveyer bottle, through adding nitrogen gas in to airtight proof box, make the air in airtight proof box follow the blast pipe discharge, thereby discharge the oxygen of inside fast, make incasement oxygen content descend, cause the state of experimental animal intermittent type nature oxygen deficiency, establish the animal model of chronic oxygen deficiency, can realize fast, thereby improve experimental speed and experimental efficiency, furthermore, through the exhaust acceleration rate case that sets up at the end of giving vent to anger of blast pipe, and the exhaust acceleration rate fan that sets up in inside, can accelerate carminative speed, thereby improve experimental speed and experimental efficiency.
2. The delivery outlet department of nitrogen gas delivery bottle is provided with threaded connection head in this chronic intermittent type nature oxygen deficiency case of numerical control, and the one end inner wall that nitrogen gas delivery pipe and nitrogen gas delivery bottle are connected is female thread structure, and with threaded connection head threaded connection, be convenient for install and dismantle, and sealed effectual, the wire through-hole has been seted up at the top of exhaust acceleration rate case, is convenient for connect the power with the inside exhaust acceleration rate fan.
3. The sealed lid installation screw that a plurality of even equidistance were arranged is seted up at the top of proof box in this chronic intermittent type nature of numerical control oxygen deficiency case, sealed lid is installed at the top of proof box, the edge of sealed lid is seted up and is equal with sealed lid installation screw quantity, the connecting hole of the corresponding just size looks adaptation in position, sealed lid passes connecting hole and sealed lid installation screw threaded connection in the top of proof box in proper order through fixing bolt, the convenience is installed and is dismantled sealed lid, fixing bolt can improve sealed effect simultaneously.
4. The numerical control chronic intermittent hypoxia chamber can control the flow of the air inlet valve and the air exhaust valve through the design of a computer application program, so that the oxygen content in the test chamber can automatically simulate the oxygen deficiency state in a body according to the experiment requirement.
Drawings
FIG. 1 is a schematic structural view of the present invention;
FIG. 2 is a schematic view of the structure of the test chamber of the present invention without the sealing cover;
FIG. 3 is a schematic view of the seal of the present invention;
FIG. 4 is a schematic cross-sectional view of the exhaust speed increasing box of the present invention;
FIG. 5 is a schematic diagram of the operating principle of the DSN gas mass flow controller of the present invention;
FIG. 6 is a schematic diagram of the operation of the computer controlled valve of the present invention.
In the figure: 1. a test chamber; 11. a nitrogen conveying pipe; 12. an intake valve; 13. an exhaust pipe; 14. an exhaust valve; 15. the sealing cover is provided with a screw hole; 2. a sealing cover; 21. a sealing groove; 22. connecting holes; 3. fixing the bolt; 4. a nitrogen delivery bottle; 41. a threaded connector; 5. an exhaust speed increasing box; 51. a fixing plate; 52. an exhaust speed increasing fan; 53. and a wire through hole.
Detailed Description
The technical solutions in the embodiments of the present invention will be clearly and completely described below with reference to the embodiments of the present invention, and it is obvious that the described embodiments are only a part of the embodiments of the present invention, and not all of the embodiments. All other embodiments, which can be derived by a person skilled in the art from the embodiments given herein without making any creative effort, shall fall within the protection scope of the present invention.
In the description of the present invention, it is to be understood that the terms "center", "longitudinal", "lateral", "length", "width", "thickness", "upper", "lower", "front", "rear", "left", "right", "vertical", "horizontal", "top", "bottom", "inner", "outer", "clockwise", "counterclockwise", and the like, indicate orientations and positional relationships based on those shown in the drawings, and are used only for convenience of description and simplicity of description, and do not indicate or imply that the equipment or element being referred to must have a particular orientation, be constructed and operated in a particular orientation, and thus, should not be considered as limiting the present invention.
Referring to fig. 1-6, the present invention provides the following technical solutions:
the utility model provides a chronic intermittent type nature oxygen deficiency case of numerical control, as shown in figure 1, including proof box 1 that is cavity seal structure, proof box 1's both ends are connected with respectively with 1 inside nitrogen conveying pipe 11 and the blast pipe 13 that is linked together of proof box, be provided with air inlet valve 12 on the nitrogen conveying pipe 11, install exhaust valve 14 on the blast pipe 13, nitrogen conveying pipe 11 keeps away from one end connection of proof box 1 and has nitrogen conveying bottle 4, one end connection that proof box 1 was kept away from to blast pipe 13 has exhaust acceleration rate case 5, install fixed plate 51 in the exhaust acceleration rate case 5, fixed plate 51 is parallel with blast pipe 13 outlet cross section, the center department of fixed plate 51 inlays to establish and installs exhaust acceleration fan 52, one end that blast pipe 13 was kept away from to exhaust acceleration rate case 5.
Further, as shown in fig. 2, the output port of the nitrogen conveying bottle 4 is provided with a threaded connector 41, the inner wall of one end of the nitrogen conveying bottle 4 connected with the nitrogen conveying pipe 11 is of an internal thread structure, and is in threaded connection with the threaded connector 41, so that the installation and the disassembly are convenient, the sealing effect is good, a wire through hole 53 is formed in the top of the exhaust speed increasing box 5, and the internal exhaust speed increasing fan 52 is conveniently connected with a power supply.
Specifically, as shown in fig. 3, sealed lid installation screw 15 that a plurality of even equidistance were arranged is seted up at proof box 1's top, sealed lid 2 is installed at proof box 1's top, sealed lid 2's edge is seted up and is equallyd divide with sealed lid installation screw 15 quantity, the connecting hole 22 of the corresponding and size looks adaptation of position, sealed lid 2 passes connecting hole 22 and sealed lid installation screw 15 threaded connection in proof box 1's top in proper order through fixing bolt 3, conveniently install and dismantle sealed lid 2, fixing bolt 3 can improve sealed effect simultaneously.
In addition, the bottom of the sealing cover 2 is provided with a sealing groove 21 matched with the top size of the test box 1, so that the sealing effect can be further improved.
In specific use, as shown in fig. 5 and 6, the air inlet valve 12 and the air outlet valve 14 both adopt gas mass flow controllers, the model of the gas mass flow controller is DSN, the DSN gas mass flow controller is composed of a mass flow sensor, a flow divider channel, a flow control regulating valve, an amplification control circuit and other components, the working principle is as shown in fig. 5, the flow controller is manufactured by utilizing the heat transfer effect of flowing fluid to transfer heat to change and measure the temperature distribution of the capillary wall, and the mass flow of gas is measured by adopting the capillary heat transfer temperature difference calorimetry principle, so that the influence of temperature and pressure can be avoided. The flow signal measured by the sensor is amplified and then compared with the set voltage, the obtained difference signal is used for driving and controlling the regulating valve, and the flow passing through the channel is controlled in a closed loop mode to be equal to the set flow. The flow splitter creates a laminar flow between the primary channel and the capillary tube to amplify the flow through the flow controller. The flow output by the controller is output in a voltage form, and the full scale is 5.00V. The controller may typically be connected to a computer or flow display compatible therewith. The DSN gas mass flow controller is directly connected with a computer, a user needs to provide a power supply, an A/D converter and a D/A converter, flow display instruments are arranged on the air inlet valve 12 and the air outlet valve 14, each flow display instrument is connected with the computer or a PLC (programmable logic controller), control operation is carried out on the flow display instruments, a three-position valve control switch is arranged in each flow display instrument, and when the flow display instruments are in a closing position, the valves are closed; when the valve is set to the open position, the valve is opened to the maximum so that the gas circuit is opened or the valve is used as a flowmeter; when the valve is controlled, the gas flow is automatically controlled according to the value of the set voltage, the flow detection voltage output by the controller is in direct proportion to the mass flow flowing through the channel, and the full-scale (F.S) flow detection output voltage is + 5.00V. The mass flow controller has a flow control range of (2 to 100)% F.S (range ratio of 50:1) and a flow resolution of o.1% F.S, and as shown in fig. 6, the flow rate detection and connection to the computer a/D converter, the external control and connection to the computer D/a converter, and if an external signal is used for flow rate setting, the external setting signal should be connected to 0 to +5.00V and "0 level" (signal ground), or may be directly connected to the computer D/a converter to realize automatic control. It should be noted that the input impedance set by the flow rate is greater than 10K Ω, if the user detects the flow rate output signal (0- +5.00V), the line is led to the "flow rate detection" and "0 level" (signal zero) line of the external control signal socket, or directly connected with the analog-to-digital converter of the computer, and the +5.00V output voltage corresponds to the full-scale rated flow rate value. It is worth noting that the flow detection output current is not more than 3mA, when the valve switch is arranged at the valve control position, the user can also control the valve through the valve control line on the external control signal socket, when the valve control line is connected with +15V, the valve is closed; when the valve control line is connected with-15V, the valve is in an opening state; when the valve control line is empty, the valve is in an automatic control state, and after the power supply is cut off, the flow is automatically loaded and stopped. It is recommended to turn off the gas first and then turn off the power supply. The flow of the gas valve is controlled by the design of a computer application program, so that the oxygen content in the test box 1 can automatically simulate the oxygen deficiency state in the body according to the experiment requirements.
The chronic intermittent type nature oxygen deficiency case of numerical control of this embodiment is connected with nitrogen delivery pipe 11 and blast pipe 13 that are linked together with proof box 1 inside respectively through both ends at airtight proof box 1, and connect nitrogen delivery pipe 11 in nitrogen delivery bottle 4, through adding nitrogen gas in to airtight proof box 1, make the air in airtight proof box 1 discharge from blast pipe 13, thereby discharge the oxygen of inside fast, make incasement oxygen content descend, cause the state of experimental animal intermittent type nature oxygen deficiency, establish the animal model of chronic oxygen deficiency, can realize fast, thereby improve experimental speed and experimental efficiency, furthermore, exhaust acceleration box 5 through the end setting of giving vent to anger at blast pipe 13, and the exhaust acceleration fan 52 that sets up in inside, exhaust speed can be accelerated, thereby improve experimental speed and experimental efficiency.
According to the device, the two end parts of the closed test box are respectively connected with the nitrogen conveying pipe and the exhaust pipe which are communicated with the interior of the test box, the flow of the air inlet valve and the flow of the exhaust valve are controlled through the design of a computer application program, the nitrogen conveying pipe is connected with the nitrogen conveying bottle, and nitrogen is added into the closed test box, so that air in the closed test box is exhausted from the exhaust pipe, oxygen in the box body is rapidly exhausted, the content of oxygen in the box is reduced, an experimental animal placed in the box is caused to have an intermittent anoxic state, and an animal model of chronic intermittent anoxia is established. This device passes through the flow of computer application program design control air inlet valve and exhaust valve, can realize fast through the exhaust acceleration rate case that the end set up of giving vent to anger at the blast pipe to and the exhaust acceleration rate fan that sets up in inside, accelerate carminative speed, improve experimental speed and test efficiency, make oxygen content in the proof box according to the experimental requirement automatic simulate the state of the interior chronic intermittent type nature oxygen disappearance of human body. Thus establishing an animal model of this state for relevant disease studies.
The foregoing shows and describes the general principles, essential features, and advantages of the invention. It will be understood by those skilled in the art that the present invention is not limited to the embodiments described above, and the preferred embodiments of the present invention are described in the above embodiments and the description, and are not intended to limit the present invention. The scope of the invention is defined by the appended claims and equivalents thereof.
Claims (7)
1. The utility model provides a chronic intermittent type nature oxygen deficiency case of numerical control which characterized in that: comprises a test box (1) with a hollow sealing structure, wherein two end parts of the test box (1) are respectively connected with a nitrogen conveying pipe (11) and an exhaust pipe (13) which are communicated with the interior of the test box (1), an air inlet valve (12) is arranged on the nitrogen conveying pipe (11), an exhaust valve (14) is arranged on the exhaust pipe (13), one end part of the nitrogen conveying pipe (11) far away from the test box (1) is connected with a nitrogen conveying bottle (4), one end part of the exhaust pipe (13) far away from the test box (1) is connected with an exhaust speed increasing box (5), a fixed plate (51) is arranged in the exhaust speed increasing box (5), the fixed plate (51) is parallel to the outlet section of the exhaust pipe (13), an exhaust speed increasing fan (52) is embedded in the center of the fixed plate (51), one end part of the exhaust speed increasing box (5) far away from the exhaust pipe (13) is in an opening state.
2. The digitally controlled chronic intermittent hypoxia chamber of claim 1, wherein: the delivery port department of nitrogen gas delivery bottle (4) is provided with threaded connection head (41), the one end inner wall that nitrogen gas delivery pipe (11) and nitrogen gas delivery bottle (4) are connected is female thread structure, and with threaded connection head (41) threaded connection.
3. The digitally controlled chronic intermittent hypoxia chamber of claim 1, wherein: and the top of the exhaust speed increasing box (5) is provided with a lead through hole (53).
4. The digitally controlled chronic intermittent hypoxia chamber of claim 1, wherein: the test box is characterized in that the top of the test box (1) is provided with a plurality of sealing cover mounting screw holes (15) which are uniformly and equidistantly arranged, the top of the test box (1) is provided with a sealing cover (2), and the edge of the sealing cover (2) is provided with connecting holes (22) which are equal to the sealing cover mounting screw holes (15) in number, correspond to the sealing cover mounting screw holes in position and are adaptive to the sealing cover mounting screw holes in size.
5. The digitally controlled chronic intermittent hypoxia chamber of claim 4, wherein: the sealing cover (2) sequentially penetrates through the connecting hole (22) and the sealing cover mounting screw hole (15) through a fixing bolt (3) to be in threaded connection with the top of the test box (1).
6. The digitally controlled chronic intermittent hypoxia chamber of claim 4, wherein: the bottom of the sealing cover (2) is provided with a sealing groove (21) matched with the top of the test box (1) in size.
7. The digitally controlled chronic intermittent hypoxia chamber of claim 1, wherein: the air inlet valve (12) and the exhaust valve (14) both adopt gas mass flow controllers, flow display instruments are arranged on the air inlet valve (12) and the exhaust valve (14), and each flow display instrument is connected with a computer or a PLC.
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Cited By (3)
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CN111699983A (en) * | 2020-06-12 | 2020-09-25 | 首都医科大学宣武医院 | Multifunctional mouse hypoxia experimental device |
CN112022574A (en) * | 2020-07-28 | 2020-12-04 | 安徽鑫诺医疗设备有限公司 | Remove portable negative pressure isolation cabin |
CN114391481A (en) * | 2021-12-14 | 2022-04-26 | 中国人民解放军空军军医大学 | Can improve low pressure oxygen cabin for animal experiments of pipe connection leakproofness |
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Cited By (3)
Publication number | Priority date | Publication date | Assignee | Title |
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