CN110742018B - 一种prv感染的阳性猪场伪狂犬病净化方法 - Google Patents
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Abstract
本发明公开了一种PRV感染的阳性猪场伪狂犬病净化方法,具体涉及伪狂犬病净化技术领域,具体净化方法如下:S1、猪场的本底调查;S2、疫苗选择、免疫及效果评估;S3、育肥猪群转阴;S4、建立阴性后备种猪群;S5、建立阴性公猪群;S6、对全部猪群进行采样疫苗免疫检测,检测结果为gE野毒抗体阳性的猪群全部淘汰。本发明通过伪狂犬病阳性猪场的本底调查,疫苗选择、免疫及效果评估,育肥猪群转阴,建立阴性后备猪群和建立阴性公猪群和检测‑淘汰‑补充策略,逐步实现伪狂犬病阳性猪场的有效防控和净化。
Description
技术领域
本发明涉及伪狂犬病净化技术领域,更具体地说,本发明涉及一种PRV感染的阳性猪场伪狂犬病净化方法。
背景技术
猪伪狂犬病是有猪伪狂犬病毒(PRV)引起的猪和多种动物一种急性传染病,临床上表现发热、奇痒、脑脊髓炎为特征,对猪危害最为严重,能导致妊娠母猪流产、产死胎和木乃伊胎、新生仔猪神经症状和死亡率升高、生长育肥猪呼吸障碍等症状,目前该病分布于全世界50多个国家和地区,对养猪业的发展构成了严重的威胁,已引起世界各国的高度关注,欧、美等发达国家始终将动物疫病的防控置于国家战略中极其重要的位置,通过对疫病流行病学、病原变异、传播机制等领域开展大量的研究,创制新型疫苗和诊断试剂,利用这些关键防控产品和综合性的策略,实现了疫病的净化与根除。英国、丹麦及美国等国家,利用伪狂犬病基因缺失疫苗及配套的鉴别诊断方法剔除带毒动物,实现猪伪狂犬病的净化与根除。
伪狂犬病是以猪为主要宿主的多种动物共患的急性传染病,我国列为二类动物疫病,我国从90年代以来通过大面积接种Battha-K61疫苗,取得了卓越的防控效果,但是自从2011年至今,由于猪伪狂犬病毒发生了变异导致PRV又在国内免疫猪群中大面积暴发,给养猪业带来了巨大的经济损失,因此,如何实现在伪狂犬病毒感染的阳性猪场特别是阳性种猪场伪狂犬病有效防控和净化是国家战略中动物疫病的防控极其重要的位置。
现有技术中防控和根除伪狂犬病暴发的方法主要是使用gE基因缺失疫苗和相配套的鉴别诊断gE抗体试剂盒,国内外大多采取免疫后检测-淘汰方法,即免疫基因缺失疫苗后利用相配套的鉴别诊断gE抗体检测,检测为阳性的猪群进行淘汰,逐步降低阳性率,最终达到伪狂犬病净化,但是自从2011年至今,PRV又在国内免疫猪群中大面积暴发,其原因主要为猪伪狂犬病毒发生了变异导致流行规律发生变化和现有疫苗或疫苗免疫方案不能完全保护猪场伪狂犬病毒的感染,以及传统的免疫后检测-淘汰方法不能做到伪狂犬病的有效防控和净化,因此需要一种PRV感染的阳性猪场伪狂犬病净化方法。
发明内容
为了克服现有技术的上述缺陷,本发明的实施例提供一种PRV感染的阳性猪场伪狂犬病净化方法,通过伪狂犬病阳性猪场的本底调查,疫苗选择、免疫及效果评估,育肥猪群转阴,建立阴性后备猪群和建立阴性公猪群和检测-淘汰-补充策略,逐步实现伪狂犬病阳性猪场的有效防控和净化。
为实现上述目的,本发明提供如下技术方案:一种PRV感染的阳性猪场伪狂犬病净化方法,具体净化方法如下:
S1、猪场的本底调查:对公猪、后备母猪、经产母猪和保育猪等不同阶段进行抗原和抗体检测,摸清伪狂犬病毒感染的规律;
S2、疫苗选择、免疫及效果评估:
S2.1、对猪群进行伪狂犬病毒基因缺失疫苗免疫;
S2.1、采集猪的全血,然后从采全血中分离血清检测伪狂犬病毒gB和gE抗体;
S2.2、使用病毒抗体中和试验检测伪狂犬中和抗体;
S2.3、通过大量数据监测表明,猪伪狂犬病阳性场发病规律为:10-12周仔猪会出现伪狂犬阳转,所以疫苗免疫效果评判标准:疫苗免疫后,通过计算大于10周龄猪群阳转率判断疫苗的免疫效果;
S2.4、通过疫苗免疫后的gB和gE抗体、中和抗体和育肥猪群野毒抗体转阳率作为评价疫苗免疫效果;
S3、育肥猪群转阴:
S3.1、育肥猪群清群:存栏的育肥猪群全部清群,然后对使用过的猪舍进行彻底的清洗消毒;
S3.2、分点饲养:将断奶仔猪分点饲养,减少了交叉感染的机会,提高了猪群的安全性;
S3.3、制定合理免疫程序,切断育肥猪群的感染,实现育肥猪群转阴;
S4、建立阴性后备种猪群:从本场育肥猪群中选择合适的阴性猪群作为后备种猪群并进行gE野毒抗体检测,剔除gE野毒抗体阳性的猪只,而gE野毒抗体阴性的猪只则保留隔离观察,并且定期对其监测,若监测发现出现gE野毒抗体阳性的猪只就直接淘汰,直到剩下的猪只全部为gE野毒抗体阴性;
S5、建立阴性公猪群:采集公猪的血清,对公猪进行gE野毒抗体检测,淘汰gE野毒抗体阳性的公猪,保留gE野毒抗体阴性的公猪,加强血清和公猪精液的定期监测,伪狂犬野毒阳性的公猪全部淘汰,保留伪狂犬野毒阴性公猪;
S6、对全部猪群进行采样检测,gE野毒抗体阳性的猪群全部淘汰。
在一个优选地实施方式中,在步骤S1中使用PCR和ELISA方法进行抗原和抗体检测。
在一个优选地实施方式中,在步骤S2.1中使用ELISA方法检测伪狂犬病毒gB和gE抗体。
在一个优选地实施方式中,在步骤S2.2中使用病毒抗体中和试验检测伪狂犬病毒中和抗体。
在一个优选地实施方式中,在步骤S3.1中清群后清洗消毒空栏30天开始正常进下一批猪。
在一个优选地实施方式中,在步骤S3.1中猪舍消毒步骤具体为:先清理猪舍残留的饲料和粪便,然后使用高压水枪对猪舍进行冲洗,5%烧碱和10%石灰水彻底消毒2-3次,然后使用高锰酸钾和甲醛对猪舍进行气体熏蒸消毒,气体熏蒸消毒后24h后猪舍开窗通风,料槽清水冲洗、药物浸泡,最后火焰消毒,饮水线和饮水嘴用特定的消毒药消毒2-3次。
本发明的技术效果和优点:
1、本发明通过伪狂犬病阳性猪场的本底调查,疫苗选择、免疫及效果评估,育肥猪群转阴,建立阴性后备猪群和建立阴性公猪群和检测-淘汰-补充策略,逐步实现伪狂犬病阳性猪场的有效防控和净化;
2、本发明通过先清理猪舍残留的饲料和粪便,然后使用高压水枪对猪舍进行冲洗,5%烧碱和10%石灰水彻底消毒2-3次,然后使用高锰酸钾和甲醛对猪舍进行气体熏蒸消毒,气体熏蒸消毒后24h后猪舍开窗通风,料槽清水冲洗、药物浸泡,最后火焰消毒,饮水线和饮水嘴用特定的消毒药消毒2-3次,有效保持猪舍的卫生以及切断病毒的传播途径,进而有效降低猪群感染伪狂犬病毒的概率。
附图说明
图1为本发明的四个厂家伪狂犬病疫苗免疫效果评估图。
具体实施方式
下面将结合本发明中的实施例,对本发明实施例中的技术方案进行清楚、完整地描述,显然,所描述的实施例仅仅是本发明一部分实施例,而不是全部的实施例。基于本发明中的实施例,本领域普通技术人员在没有做出创造性劳动前提下所获得的所有其他实施例,都属于本发明保护的范围。
实施例1:伪狂犬病阳性猪场的本底调查
(1)实施时间:2017年1月份到2018年10月份。
(2)样品采集:广东某猪场采集样品共1298份(其中血清样品1214份,公猪精液66份,死胎15份,仔猪组织样品3份),
(3)方法:通过ELISA对伪狂犬gB抗体和伪狂犬gE抗体进行检测,RT-PCR方法对伪狂犬病毒抗原进行检测。
(4)本底调查结果
种猪(包括公猪、后备母猪和经产母猪)的伪狂犬gB抗体阳性率为91.7%-100%,仔猪(20d-150d)伪狂犬gB抗体阳性率为4.8%-100%。种猪(包括公猪、后备母猪和经产母猪)的伪狂犬gE抗体阳性率为23.7%-77.8%,仔猪(20d-150d)伪狂犬gE抗体阳性率为0%-57.8%。抗原检测表明,共采集了公猪精液、死胎、产房发病仔猪进行RT-PCR检测,结果都为阴性。
实施例2:疫苗免疫效果评估
育肥猪群野毒抗体转阳率作为评价疫苗免疫效果:通过大量数据监测表明,猪伪狂犬病阳性场发病规律为:10-12周仔猪会出现伪狂犬阳转,所以疫苗免疫效果评判标准:疫苗免疫后,通过计算大于10周龄猪群阳转率判断疫苗的免疫效果。
1)试验实施起止时间:2017年4月-11月。
2)疫苗免疫:分别用市场上四个厂家伪狂犬病缺失疫苗免疫8周龄仔猪,12周龄加强免疫。
3)采血方法及采血量:耳静脉采血或颈静脉采血,仔猪每份样品采血应不少于3-5毫升,静置平放15~20分钟,充分析出血清后4~8℃冷藏保存送检或-20℃保存待检。
4)免疫效果
通过2批仔猪疫苗免疫效果表明,4个厂家疫苗免疫130日龄后仔猪阳转率为0,说明疫苗能够阻断病毒感染,疫苗免疫效果越好。
实施例3:建立阴性后备母猪群
采取免疫-检测-淘汰策略后,通过定期检测保留阴性后备猪,淘汰阳性后备猪。
广东某猪场2017年4月份开始摸底调查和实施猪伪狂犬病净化项目,通过免疫-检测-淘汰策略,从方案实施半年后猪场后备母猪伪狂犬病gE阳性率从77.8%下降为0%。定期采样监测,后备猪伪狂犬病一直保持阴性,证明成功建立阴性后备猪群。
实施例4:建立建立阴性公猪群
广东某猪场通过免疫-检测-淘汰策略,猪场公猪伪狂犬病gE阳性率从66.7%下降为0%。通过RT-PCR方法定期检测公猪精液,结果阴性的精液才能使用,阻断伪狂犬病毒通过公猪精液传播给母猪的风险。
实施例5:检测-淘汰策略
对全部猪群进行采样检测,gE野毒抗体阳性的猪群全部淘汰。
最后:以上所述仅为本发明的优选实施例而已,并不用于限制本发明,凡在本发明的精神和原则之内,所作的任何修改、等同替换、改进等,均应包含在本发明的保护范围之内。
Claims (5)
1.一种PRV感染的阳性猪场伪狂犬病净化方法,其特征在于:具体净化方法如下:
S1、猪场的本底调查:对公猪、后备母猪、经产母猪和保育猪不同阶段进行抗原和抗体检测,摸清伪狂犬病毒感染的规律;
S2、疫苗选择、免疫及效果评估:
S2.1、对猪群进行伪狂犬病毒基因缺失疫苗免疫;
S2.1、采集猪的全血,然后从采全血中分离血清检测伪狂犬病毒gB和gE抗体;
S2.2、使用病毒抗体中和试验检测伪狂犬中和抗体;
S2.3、通过大量数据监测表明,猪伪狂犬病阳性场发病规律为:10-12周仔猪会出现伪狂犬阳转,所以疫苗免疫效果评判标准:疫苗免疫后,通过计算大于10周龄猪群阳转率判断疫苗的免疫效果;
S2.4、通过疫苗免疫后的gB和gE抗体、中和抗体和育肥猪群野毒抗体转阳率作为评价疫苗免疫效果;
S3、育肥猪群转阴:
S3.1、育肥猪群清群:存栏的育肥猪群全部清群,然后对使用过的猪舍进行彻底的清洗消毒,步骤具体为:先清理猪舍残留的饲料和粪便,然后使用高压水枪对猪舍进行冲洗,5%烧碱和10%石灰水彻底消毒2-3次,然后使用高锰酸钾和甲醛对猪舍进行气体熏蒸消毒,气体熏蒸消毒后24h后猪舍开窗通风,料槽清水冲洗、药物浸泡,最后火焰消毒,饮水线和饮水嘴用消毒药消毒2-3次;
S3.2、分点饲养:将断奶仔猪分点饲养,减少了交叉感染的机会,提高了猪群的安全性;
S3.3、制定合理免疫程序,切断育肥猪群的感染,实现育肥猪群转阴;
S4、建立阴性后备种猪群:从本场育肥猪群中选择合适的阴性猪群作为后备种猪群并进行gE野毒抗体检测,剔除gE野毒抗体阳性的猪只,而gE野毒抗体阴性的猪只则保留隔离观察,并且定期对其监测,若监测发现出现gE野毒抗体阳性的猪只就直接淘汰,直到剩下的猪只全部为gE野毒抗体阴性;
S5、建立阴性公猪群:采集公猪的血清,对公猪进行gE野毒抗体检测,淘汰gE野毒抗体阳性的公猪,保留gE野毒抗体阴性的公猪,加强血清和公猪精液的定期监测,伪狂犬野毒阳性的公猪全部淘汰,保留伪狂犬野毒阴性公猪;
S6、对全部猪群进行采样检测,gE野毒抗体阳性的猪群全部淘汰。
2.根据权利要求1所述的一种PRV感染的阳性猪场伪狂犬病净化方法,其特征在于:在步骤S1中使用PCR和ELISA方法进行抗原和抗体检测。
3.根据权利要求1所述的一种PRV感染的阳性猪场伪狂犬病净化方法,其特征在于:在步骤S2.1中使用ELISA方法检测伪狂犬病毒gB和gE抗体。
4.根据权利要求1所述的一种PRV感染的阳性猪场伪狂犬病净化方法,其特征在于:在步骤S2.2中使用病毒抗体中和试验检测伪狂犬病毒中和抗体。
5.根据权利要求1所述的一种PRV感染的阳性猪场伪狂犬病净化方法,其特征在于:在步骤S3.1中清群后清洗消毒空栏30天开始正常进下一批猪。
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