CN110741932A - Ginger detoxification tissue culture device and culture method - Google Patents
Ginger detoxification tissue culture device and culture method Download PDFInfo
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- CN110741932A CN110741932A CN201911108507.8A CN201911108507A CN110741932A CN 110741932 A CN110741932 A CN 110741932A CN 201911108507 A CN201911108507 A CN 201911108507A CN 110741932 A CN110741932 A CN 110741932A
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- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01H—NEW PLANTS OR NON-TRANSGENIC PROCESSES FOR OBTAINING THEM; PLANT REPRODUCTION BY TISSUE CULTURE TECHNIQUES
- A01H4/00—Plant reproduction by tissue culture techniques ; Tissue culture techniques therefor
- A01H4/001—Culture apparatus for tissue culture
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- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01H—NEW PLANTS OR NON-TRANSGENIC PROCESSES FOR OBTAINING THEM; PLANT REPRODUCTION BY TISSUE CULTURE TECHNIQUES
- A01H4/00—Plant reproduction by tissue culture techniques ; Tissue culture techniques therefor
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Abstract
The invention discloses a ginger detoxification tissue culture device, which comprises a box body base, an incubator, a sealing shell and a fixed bottom box, wherein the sealing shell is fixedly arranged on the outer surface of the upper end of the box body base, the outer surface of one side of the sealing shell is fixedly provided with an agitator tank, the outer surface of the upper part of the agitator tank is fixedly sleeved with a fixed clamping seat, the inner side of the fixed clamping seat is fixedly sleeved with a telescopic rod, the fixed clamping seat and the sealing shell are fixedly butted and fixed through the telescopic rod, the inner surface of the inner side of the agitator tank is movably sleeved with a telescopic sleeve, the outer surface of the telescopic sleeve is provided with a limiting sliding chute, the incubator is fixedly arranged on one side, close to the sealing shell, of the outer surface of the upper end; make this be used for ginger detoxification group to organize device have combination formula fixed knot to construct, promote the flexibility ratio when it uses, make it have supplementary feed structure simultaneously, reduce the operation step when it uses.
Description
Technical Field
The invention belongs to the technical field of tissue culture devices, particularly relates to a ginger virus-free tissue culture device, and more particularly relates to a ginger virus-free tissue culture device and a ginger virus-free tissue culture method.
Background
This be used for ginger detoxification to organize device with earth is a ginger culture apparatus that the operation of cultivating was planted to ginger detoxification, utilizes this ginger detoxification group to organize the device with earth, can carry out corresponding regulation operation to temperature, humidity and illumination intensity when ginger detoxification cultivation operation, cultivates for ginger detoxification and plants the operation and promote suitable growing environment.
A culture device disclosed by a comparison document CN209498155U relates to the technical field of biological culture. The culture apparatus includes: the device comprises a culture box, a culture box cover, two transparent supporting plates, a transparent loading plate and a plurality of culture chambers for loading seeds; the culture box cover is used for covering or opening the culture box; the two transparent supporting plates are vertically arranged in the culture box, the transparent loading and carrying plate is transversely arranged in the culture box and is detachably connected with the two transparent supporting plates respectively, and the position of the transparent loading and carrying plate on the two transparent supporting plates is adjustable along the height of the culture box; the plurality of culture chambers are connected on the transparent loading plate in a hanging manner; is positioned at the top of the culture chamber; compared with the invention, the split joint type fixing structure is not provided, thereby reducing the flexibility of the device in use.
The conventional ginger virus-free tissue culture device has certain defects in the use process, and the conventional ginger virus-free tissue culture device adopts an integrated fixed structure design, so that the ginger virus-free tissue culture device cannot be adjusted freely according to the size of a ginger block to be cultured, and the flexibility of the ginger virus-free tissue culture device in use is reduced; meanwhile, the traditional ginger detoxification tissue culture device does not have an auxiliary feeding structure, so that a user needs to perform nutrient adding operation on the ginger detoxification tissue culture device in a traditional manual mode in the ginger detoxification culture process, and operation steps of the user are increased; secondly the tradition is used for ginger detoxification group to organize the device and does not have concatenation formula fixed knot to increased this a ginger detoxification group that is used for organizes the degree of difficulty when device maintains and changes the operation, brought certain adverse effect for the user.
Disclosure of Invention
In order to overcome the defect that the traditional ginger virus-free tissue culture device adopts an integrated fixed structure design, the ginger virus-free tissue culture device cannot be adjusted randomly according to the size of ginger blocks to be cultured, and the flexibility of the ginger virus-free tissue culture device in use is reduced; meanwhile, the traditional ginger detoxification tissue culture device does not have an auxiliary feeding structure, so that a user needs to perform nutrient adding operation on the ginger detoxification tissue culture device in a traditional manual mode in the ginger detoxification culture process, and operation steps of the user are increased; secondly the traditional ginger detoxification tissue culture device does not have a splicing type fixed structure, thereby increasing the difficulty of the ginger detoxification tissue culture device in maintenance and replacement operation, and providing the ginger detoxification tissue culture device.
The purpose of the invention is realized by the following technical scheme:
a tissue culture device for ginger detoxification comprises a box body base, an incubator, a sealing shell and a fixed bottom box, wherein the sealing shell is fixedly arranged on the outer surface of the upper end of the box body base, an agitator tank is fixedly arranged on the outer surface of one side of the sealing shell, a fixed clamping seat is fixedly sleeved on the outer surface of the upper portion of the agitator tank, a telescopic rod is fixedly sleeved on the inner side of the fixed clamping seat, the fixed clamping seat and the sealing shell are fixedly butted through the telescopic rod, a telescopic sleeve is movably sleeved on the inner surface of the inner side of the agitator tank, a limiting sliding chute is formed in the outer surface of the telescopic sleeve, the incubator is fixedly arranged on the outer surface of the upper end of the fixed bottom box, which is close to one side of the sealing shell, the fixed bottom box is fixedly arranged on the inner surface of the bottom of the incubator, a combined, the outer surface of the lower end of the separation clamping plate is fixedly provided with a pressing base plate, the inner surface of the inner side of the separation clamping plate is movably provided with a movable clamping rod, the outer surface of one end of the movable clamping rod is fixedly provided with a spring pin, and the outer surface of the upper end of the movable clamping rod is fixedly provided with a third sliding buckle.
As a further scheme of the invention, the whole structure of the stirring tank is a cylindrical two-section structure, the stirring tank and the telescopic sleeve are fixedly butted through a limiting sliding groove, two groups of sucker supporting feet are fixedly installed at the bottom of the sealing shell, the sealing shell and the base of the box body are fixedly butted through the sucker supporting feet, the fixed bottom box and the incubator are fixedly butted through a combined bottom plate, and the separation clamping plate and the movable clamping rod are elastically connected through a spring pin.
As a further scheme of the invention, a water collecting tank is fixedly arranged on the outer surface of the upper end of the culture tank, two groups of sealing covers are fixedly arranged on the outer surface of the front end of the water collecting tank, a liquid collecting hopper is fixedly arranged at the bottom of the water collecting tank, a partition plate is fixedly arranged in the middle of the inner surface of the water collecting tank, two shutoff plates are movably arranged on the inner surface of the water collecting tank close to two sides of the partition plate, two groups of second sliding buckles are movably arranged on the front part of the water collecting tank, the shutoff plates and the second sliding buckles are fixed by welding, a communicating pipe is fixedly arranged at the lower end of the liquid collecting hopper.
As a further scheme of the invention, two groups of fixed chucks are movably mounted on the outer surface of the upper end of the combined bottom plate, the combined bottom plate and the fixed bottom box are fixedly butted through the fixed chucks, two groups of first sliding buckles are movably mounted on the front portion of the combined bottom plate, the fixed chucks and the first sliding buckles are fixedly butted through clamping grooves, four groups of fixed bottom bolts are fixedly sleeved at the bottom of the combined bottom plate, the outer surfaces of two sides of the separation clamping plate are of arc structures, and one side of each fixed chuck is provided with a butting clamping groove.
As a further scheme of the invention, a side-turning cover is movably arranged on one side of the sealing shell close to the upper part of the stirring tank, the sealing shell and the side-turning cover are fixedly butted through a rotating clamping frame, a motor is fixedly sleeved on the inner side of the sealing shell, a driving rod is fixedly arranged at the lower end of the motor, a rotating stirring rod is fixedly arranged at the lower end of the driving rod, and the motor and the rotating stirring rod are fixedly butted through the driving rod.
As a further scheme of the invention, two groups of container boxes are fixedly arranged on the other side of the outer surface of the upper end of the box body base close to the sealing shell, a cleaning basin is fixedly arranged on the outer surface of one side of the box body base, a connecting pipe is fixedly arranged on the cleaning basin, a drainage pipe head is fixedly arranged on the outer surface of the lower end of the connecting pipe, and a spray head is fixedly arranged on the outer surface of the upper end of the connecting pipe.
As a further scheme of the invention, an induced draft fan is fixedly installed on the outer surface of the side edge of the incubator, a plurality of groups of ventilation grooves are formed in the inner side of the upper portion of the incubator in a penetrating mode, two groups of lamp tubes are fixedly installed inside the incubator, a fixed vertical plate is fixedly installed at the upper end of a base of the incubator body, a rotating top plate is movably installed on the upper portion of the fixed vertical plate, the rotating top plate and the fixed vertical plate are fixedly connected through a hinge in a butt joint mode, and a fluorescent lamp tube is fixedly installed on the outer.
As a further scheme of the invention, four groups of supporting legs are fixedly arranged on the outer surface of the bottom of the box body base, sliding trundles are fixedly arranged on one side, close to the supporting legs, of the bottom of the box body base, a rotating supporting plate is movably arranged on the outer surface of the front end of the box body base, and the box body base and the rotating supporting plate are supported and fixed through foot stands.
As a further scheme of the invention, the inner surface of the upper part of the fixed bottom box is provided with a planting groove, and the inner surface of the inner side of the three-way pipe is provided with a circular notch.
A culture method for a ginger virus-free tissue culture device comprises the following specific operation steps:
pulling the stirring tank downwards by using a limiting sliding chute between the stirring tank and the telescopic sleeve, drawing out the telescopic sleeve, turning over a side turning cover through a rotating clamping frame, opening the upper part of the stirring tank, pouring a culture medium raw material into the stirring tank, closing the side turning cover, starting a motor, driving the motor to rotate a rotating stirring rod through a driving rod, opening two groups of sealing covers of a water collecting tank, and respectively pouring a mixed culture medium and a water body into the water collecting tank;
placing the ginger blocks in a container box, covering fine sand soaked by carbendazim in the container box, placing the ginger blocks in a fixed bottom box for culturing, inserting a separation clamping plate in the fixed bottom box according to the size of the ginger blocks, pushing a movable clamping rod to clamp and fix the separation clamping plate by using a spring pin, covering soil on the fixed bottom box, pushing a second sliding buckle of a water collecting box, opening a cut-off plate, and enabling a culture medium and a water body to enter a communicating pipe through a liquid collecting hopper, be discharged from a circular notch of a three-way pipe and enter the fixed bottom box;
and step three, when the buds of the ginger blocks grow to 2 cm, taking out the ginger blocks in the fixed bottom box, sterilizing the ginger blocks for seven minutes through mercuric chloride, placing the ginger blocks in the incubator again, modulating the light intensity of a lamp tube in the incubator to 4000 lux, adjusting the illumination time to 12 hours every day, after the cultivation is finished, placing the ginger blocks in a cleaning basin, and washing the ginger blocks with sterile water through a spray head of a connecting tube.
The invention has the beneficial effects that:
1. by arranging the separation clamping plate, when a user utilizes the ginger detoxification tissue culture device to culture ginger, the user can utilize the arrangement of the fixed bottom box to carry out containing operation on ginger blocks to be cultured, when the user needs to utilize the fixed bottom box to contain a plurality of groups of ginger blocks with different sizes, the user can insert the separation clamping plate in the fixed bottom box according to the sizes of the ginger blocks, the user can move the movable clamping rod to the inside of the separation clamping plate by the third sliding buckle of the movable clamping rod, the resistance between the separation clamping plate and the fixed bottom box can be reduced when the separation clamping plate is inserted into the fixed bottom box by utilizing the arc-shaped structures on the outer surfaces of the two sides of the separation clamping plate, so that the inserting operation of the separation clamping plate is smoother, when the separation clamping plate is inserted into the fixed bottom box, the user can release the third sliding buckle to enable the spring pin to rebound the movable clamping rod, and pop the movable clamping, make the removal kelly top on fixed end box to separating the cardboard after inserting and playing the fixed action, the cooperation is separated the backing plate that sticiss of cardboard bottom, can separate the cardboard and insert the back, promote its leakproofness, utilize the setting of separating the cardboard, make this a group banks up device with earth for ginger detoxification have concatenation formula fixed knot and construct, make it can adjust its growth space wantonly according to the ginger piece size, thereby effectively promote the flexibility ratio of its when using.
2. By arranging the three-way pipe and the water collecting tank, when a user needs to irrigate nutrient in the process of cultivation, the user can pour mixed culture medium and water into the water collecting tank respectively by opening the sealing cover of the water collecting tank, the user can push the second slide fastener of the water collecting tank and open the cut-off plate by using the second slide fastener, so that the culture medium and the water enter the communicating pipe through the liquid collecting hopper, enter the three-way pipe through the communicating pipe and then are discharged from the circular notch of the three-way pipe and enter the fixed bottom box, thereby completing nutrient supply operation in the process of ginger detoxification cultivation, the nutrient supply operation in the process of ginger detoxification cultivation can be simplified by using the arrangement of the three-way pipe and the water collecting tank, when the user needs to irrigate nutrient supply operation to the ginger, the user only needs to open the cut-off plate and directly fill the culture medium and the water into soil by using the three-way pipe, the ginger detoxification tissue culture device has an auxiliary feeding structure, so that the operation steps of a user are effectively simplified.
3. Through setting up the built-up plate, need change the operation to this a fixed end box that is used for ginger detoxification group to organize device at the time of, the user can be through the first thread slipping that promotes the built-up plate, make the fixed dop of first thread slipping drive remove, thereby make the bottom separation of fixed dop and fixed end box, make fixed end box can take off from the built-up plate, change not equidimension fixed end box after, place again on the built-up plate, utilize fixed dop card fastening fixed end box, setting through the built-up plate, make this ginger detoxification group to organize device with earth to have combination formula fixed knot, thereby reduce its operation degree of difficulty when dismantling the installation, convenient to use person maintains it.
4. Through setting up the expansion sleeve, when the user needs required culture medium when detoxicating ginger to mix the operation, the user can utilize the spacing spout between agitator tank and the expansion sleeve to stimulate the agitator tank downwards, takes the expansion sleeve out, utilizes the expansion sleeve after taking out to adjust the inside degree of depth of agitator tank to make the agitator tank can carry out arbitrary regulation operation according to the quantity of culture medium, promote the flexibility ratio when this ginger detoxification tissue culture device uses.
Drawings
The invention will be further described with reference to the accompanying drawings.
FIG. 1 is a schematic view of the overall structure of a ginger detoxification tissue culture device of the present invention.
FIG. 2 is an overall structure diagram of a sealed shell of the ginger virus-free tissue culture device of the invention.
FIG. 3 is an overall structure diagram of a fixed bottom box of the ginger detoxification tissue culture device of the invention.
FIG. 4 is an overall structure diagram of a water collection tank of the ginger detoxification tissue culture device of the invention.
FIG. 5 is an internal structure diagram of a separating plate of a ginger detoxification tissue culture device of the present invention.
FIG. 6 is an overall structure diagram of a stirring tank of the ginger detoxification tissue culture device.
In the figure: 1. a sliding caster; 2. supporting legs; 3. a base of the box body; 4. moving the clamping rod; 5. rotating the supporting plate; 6. fixing the bottom box; 7. an induced draft fan; 8. a separation clamping plate; 9. an incubator; 10. a sealing cover; 11. a water collection tank; 12. rotating the top plate; 13. a fluorescent lamp tube; 14. fixing the vertical plate; 15. a connecting pipe; 16. a container box; 17. sealing the housing; 18. a wash basin; 19. a drain pipe head; 20. a suction cup arm brace; 21. a stirring tank; 22. rotating the stirring rod; 23. a drive rod; 24. an electric motor; 25. laterally turning the flip; 26. rotating the clamping frame; 27. fixing a bottom bolt; 28. a first slide fastener; 29. fixing the chuck; 30. a combined bottom plate; 31. pressing the base plate; 32. a communicating pipe; 33. a liquid collecting hopper; 34. a second slide fastener; 35. a shutoff plate; 36. a three-way pipe; 37. a third slide fastener; 38. a spring pin; 39. a limiting chute; 40. a telescopic rod; 41. fixing the card holder; 42. a telescopic sleeve.
Detailed Description
The technical solutions in the embodiments of the present invention will be clearly and completely described below with reference to the embodiments of the present invention, and it is obvious that the described embodiments are only a part of the embodiments of the present invention, and not all of the embodiments. All other embodiments, which can be derived by a person skilled in the art from the embodiments given herein without making any creative effort, shall fall within the protection scope of the present invention.
As shown in figures 1-6, a tissue culture device for ginger detoxification comprises a box base 3, an incubator 9, a seal housing 17 and a fixed bottom box 6, wherein the seal housing 17 is fixedly installed on the outer surface of the upper end of the box base 3, an agitator tank 21 is fixedly installed on the outer surface of one side of the seal housing 17, a fixed clamping seat 41 is fixedly sleeved on the outer surface of the upper portion of the agitator tank 21, an expansion link 40 is fixedly sleeved on the inner side of the fixed clamping seat 41, the fixed clamping seat 41 and the seal housing 17 are fixedly butted and connected through the expansion link 40, an expansion link 42 is movably sleeved on the inner surface of the inner side of the agitator tank 21, a limit sliding chute 39 is formed on the outer surface of the expansion link 42, the incubator 9 is fixedly installed on the outer surface of the upper end of the fixed bottom box 6 close to one side of the seal housing 17, the fixed bottom box 6 is fixedly, the inboard fixed mounting in upper portion of fixed end box 6 separates cardboard 8, separates the lower extreme external surface fixed mounting of cardboard 8 and sticiss backing plate 31, separates inboard internal surface movable mounting of cardboard 8 and has the removal kelly 4, and the one end external surface fixed mounting of removal kelly 4 has spring catch 38, and the upper end external surface fixed mounting of removal kelly 4 has third thread slipping 37.
The overall structure of agitator tank 21 is two segmentation structures of cylinder, utilize two segmentation structures can be so that agitator tank 21 cooperation telescopic sleeve 42 accomplishes flexible removal, and it is fixed to dock through spacing spout 39 between agitator tank 21 and the telescopic sleeve 42, seal shell 17's bottom fixed mounting has two sets of sucking disc spike 20, and it is fixed to dock through sucking disc spike 20 between seal shell 17 and the box base 3, dock fixedly through built-up plate 30 between solid bottom box 6 and the incubator 9, separate through spring catch 38 elastic connection between cardboard 8 and the removal kelly 4.
The outer surface of the upper end of the incubator 9 is fixedly provided with a water collecting tank 11, the outer surface of the front end of the water collecting tank 11 is fixedly provided with two groups of sealing covers 10, the bottom of the water collecting tank 11 is fixedly provided with a liquid collecting hopper 33, the middle position of the inner surface of the water collecting tank 11 is fixedly provided with a partition plate, the inner surface of the water collecting tank 11 is movably provided with cut-off plates 35 close to the two sides of the partition plate, the cut-off plates 35 play a role in controlling the flow of water and culture medium in the water collecting tank 11, the front part of the water collecting tank 11 is movably provided with two groups of second sliding buckles 34, the cut-off plates 35 and the second sliding buckles 34 are fixed by welding, the lower end of the liquid collecting hopper 33 is fixedly provided with a communicating pipe 32, the bottom of the communicating pipe 32 is fixedly provided with a three-.
The upper end surface movable mounting of built-up plate 30 has two sets of fixed dops 29, and it is fixed to dock through fixed dop 29 between built-up plate 30 and the fixed end box 6, the anterior movable mounting of built-up plate 30 has two sets of first slipbuckles 28, it is fixed to dock through the draw-in groove between fixed dop 29 and the first slipbuckle 28, the fixed dead bolt 27 of four groups that has cup jointed in bottom of built-up plate 30, the both sides surface of separating cardboard 8 is the arc structure, the design that utilizes the arc structure can reduce the resistance when separating cardboard 8 and inserting, the butt joint draw-in groove has been seted up to one side of fixed dop 29.
One side of seal shell 17 is close to the upper portion movable mounting of agitator tank 21 and is had side flip 25, and seal shell 17 and side between the flip 25 butt joint fixedly through rotatory card frame 26, the inboard fixed cup joint of seal shell 17 has motor 24, and the lower extreme fixed mounting of motor 24 has actuating lever 23, the lower extreme fixed mounting of actuating lever 23 has rotatory stirring rod 22, the rotation that utilizes to change stirring rod 22 can carry out mixing operation to the culture medium, it is fixed through actuating lever 23 butt joint between motor 24 and the rotatory stirring rod 22.
Two sets of container boxes 16 are fixedly mounted on the outer surface of the upper end of the box base 3 close to the other side of the sealing shell 17, a cleaning basin 18 is fixedly mounted on the outer surface of one side of the box base 3, a connecting pipe 15 is fixedly mounted on the cleaning basin 18, a drainage pipe head 19 is fixedly mounted on the outer surface of the lower end of the connecting pipe 15, and a spray head is fixedly mounted on the outer surface of the upper end of the connecting pipe 15.
The side surface fixed mounting of incubator 9 has draught fan 7, operation cooperation ventilation groove through draught fan 7, can be to the inside operation of ventilating that ventilates of incubator 9, and incubator 9's upper portion inboard runs through and has seted up a plurality of ventilation grooves of group, incubator 9's inside fixed mounting has two sets of fluorescent tubes, the upper end fixed mounting of box base 3 has fixed riser 14, and the upper portion movable mounting of fixed riser 14 has rotatory roof 12, it is fixed through the hinge butt joint between rotatory roof 12 and the fixed riser 14, the bottom surface fixed mounting of rotatory roof 12 has fluorescent tube 13.
Four groups of supporting legs 2 are fixedly mounted on the outer surface of the bottom of the box base 3, sliding trundles 1 are fixedly mounted on one side, close to the supporting legs 2, of the bottom of the box base 3, rotary supporting plates 5 are movably mounted on the outer surface of the front end of the box base 3, and the box base 3 and the rotary supporting plates 5 are fixedly supported through foot stands.
The inner surface of the upper part of the fixed bottom box 6 is provided with a planting groove, and the inner surface of the inner side of the three-way pipe 36 is provided with a circular notch.
A culture method for a ginger virus-free tissue culture device comprises the following specific operation steps:
firstly, pulling the stirring tank 21 downwards by using a limiting sliding chute 39 between the stirring tank 21 and the telescopic sleeve 42, drawing out the telescopic sleeve 42, turning over the side turning cover 25 by using a rotary clamping frame 26, opening the upper part of the stirring tank 21, pouring a culture medium raw material into the stirring tank 21, closing the side turning cover 25, starting a motor 24, driving the rotary stirring rod 22 to rotate by using the motor 24 through a driving rod 23, opening two groups of sealing covers 10 of a water collecting tank 11, and pouring the mixed culture medium and water into the water collecting tank 11 respectively;
placing the ginger blocks in the container box 16, covering fine sand soaked by the carbendazim in the container box, placing the ginger blocks in the fixed bottom box 6 for culturing, inserting the separation clamping plate 8 into the fixed bottom box 6 according to the sizes of the ginger blocks, pushing the movable clamping rod 4 to clamp the fixed separation clamping plate 8 by using the spring pin 38, covering soil on the fixed bottom box 6, pushing the second sliding buckle 34 of the water collecting tank 11, opening the shutoff plate 35, enabling the culture medium and the water body to enter the communicating pipe 32 through the liquid collecting hopper 33, discharging the culture medium and the water body from the circular notch of the three-way pipe 36, and entering the fixed bottom box 6;
and step three, when the buds of the ginger blocks grow to 2 cm, taking out the ginger blocks in the fixed bottom box 6, sterilizing the ginger blocks for seven minutes through mercuric chloride, placing the ginger blocks in the incubator 9 again, modulating the light intensity of a lamp tube in the incubator 9 to 4000 lux, adjusting the illumination time to 12 hours every day, after the cultivation is finished, placing the ginger blocks in the cleaning basin 18, and washing the ginger blocks with sterile water through a spray head of the connecting pipe 15.
The invention aims to provide a ginger detoxification tissue culture device, when in use, the separation clamping plate 8 is arranged, when a user utilizes the ginger detoxification tissue culture device to culture ginger, the user can utilize the arrangement of the fixed bottom box 6 to carry out containing operation on ginger blocks to be cultured, when the user needs to utilize the fixed bottom box 6 to contain a plurality of groups of ginger blocks with different sizes, the user can insert the separation clamping plate 8 into the fixed bottom box 6 according to the sizes of the ginger blocks, the user can move the movable clamping rod 4 towards the inside of the separation clamping plate 8 through the third slide fastener 37 of the movable clamping rod 4, and by utilizing the arc-shaped structures on the outer surfaces of the two sides of the separation clamping plate 8, when the separation clamping plate 8 is inserted into the fixed bottom box 6, the resistance between the separation clamping plate 8 and the fixed bottom box 6 is reduced, so that the insertion operation of the separation clamping plate 8 is smoother, when the separation clamping plate 8 is inserted into the fixed bottom box 6, the user can loosen the third sliding buckle 37 to get the spring catch 38 to move the clamping rod 4, pop out the removal clamping rod 4 from separating the cardboard 8, make the removal clamping rod 4 push up on fixed end box 6, thereby play the fixed action to separating the cardboard 8 after inserting, the cooperation is separated the sticis backing plate 31 of cardboard 8 bottom in order to separate the cardboard 8 after inserting, promote its leakproofness, utilize the setting of separating cardboard 8, make this a ginger detoxification group banks up the device with earth and has a concatenation formula fixed knot structure, make it can adjust its growth space wantonly according to the ginger piece size, thereby effectively promote the flexibility ratio when its uses, through setting up three-way pipe 36 and header tank 11, when the user needs to carry out the nourishment watering operation to the ginger in the culture process, the user can be through opening the sealed lid 10 of header tank 11, pour into mixed culture medium and water body in header tank 11 respectively, the user can cut off the board 35 and get culture medium and water body with the second sliding buckle 34 through the second sliding buckle 34 that promotes header tank 11 The water body is communicated with the three-way pipe 36 through the communicating pipe 32 by the liquid collecting hopper 33 and then discharged from the groove port of the three-way pipe 36 and enters the fixed bottom box 6, so that the nutrient supply operation in the ginger detoxification culture process is completed, the nutrient supply operation in the ginger detoxification culture process can be simplified by utilizing the facilities of the three-way pipe 36 and the water collecting box 11, when a user needs to carry out the nutrient supply operation on the ginger detoxification culture device, the user only needs to open the shutoff plate 35 to directly infuse culture medium and water into soil by using the three-way pipe 36, so that the ginger detoxification tissue culture device has an auxiliary feeding structure, the operation steps of the user are effectively simplified, when the user needs to carry out the replacement operation on the fixed bottom box 6 of the ginger detoxification tissue culture device by arranging the combined bottom plate 30, the user can push the first slide fastener 28 of the combined bottom plate 30 to drive the fixed chuck 29 to move, thereby separating the fixed chuck 29 from the bottom of the fixed bottom box 6, so that the fixed bottom box 6 can be replaced by the fixed bottom box 6 of different sizes from the combined bottom plate 30, and then the fixed bottom box 6 is placed on the combined bottom plate 30 again by using the fixed chuck 29, and the combined bottom plate 30 is used, so that the ginger detoxification tissue culture device has a combined type fixed structure, thereby reducing the operation difficulty when the ginger detoxification tissue culture device is disassembled and installed, and facilitating the maintenance of a user, when the user needs to perform mixing operation on required culture mediums when ginger detoxification by setting the telescopic sleeve 42, the user can pull the stirring tank 21 by pulling the stirring tank 21, and pull the stirring tank 21 by using the limiting sliding grooves 39 of the stirring tank 21 and the telescopic sleeve 42, and adjust the inner depth of the stirring tank 21 by using the telescopic sleeve 42 after being pulled out, thereby the stirring tank 21 can perform any adjusting operation according to the number of the culture mediums, the flexibility of the ginger detoxification tissue culture device during use is improved.
The preferred embodiments of the invention disclosed above are intended to be illustrative only. Preferred embodiments are not exhaustive of all the details of the embodiments. Obviously, many modifications and variations are possible in light of the above teaching. The embodiments were chosen and described in order to best explain the principles of the invention and the practical application, to thereby enable others skilled in the art to best utilize the invention. The invention is limited only by the claims and their full scope and equivalents.
Claims (10)
1. The tissue culture device for ginger detoxification is characterized by comprising a box body base (3), an incubator (9), a sealing shell (17) and a fixed bottom box (6), wherein the sealing shell (17) is fixedly arranged on the outer surface of the upper end of the box body base (3), a stirring tank (21) is fixedly arranged on the outer surface of one side of the sealing shell (17), a fixed clamping seat (41) is fixedly sleeved on the outer surface of the upper portion of the stirring tank (21), a telescopic rod (40) is fixedly sleeved on the inner side of the fixed clamping seat (41), the fixed clamping seat (41) and the sealing shell (17) are fixedly butted and fixed through the telescopic rod (40), a telescopic sleeve (42) is movably sleeved on the inner side inner surface of the stirring tank (21), a limiting sliding chute (39) is formed in the outer surface of the telescopic sleeve (42), the incubator (9) is fixedly arranged on one side, close to the outer surface of the upper end of the fixed bottom box (6, fixed end box (6) fixed mounting is in the bottom internal surface of incubator (9), the lower extreme external fixed surface of fixed end box (6) installs built-up plate (30), the inboard fixed mounting in upper portion of fixed end box (6) separates cardboard (8), the lower extreme external fixed surface who separates cardboard (8) installs and sticiss backing plate (31), the inboard internal surface movable mounting who separates cardboard (8) has removal kelly (4), the one end external fixed surface of removal kelly (4) installs spring catch (38), the upper end external fixed surface of removal kelly (4) installs third thread slipping (37).
2. The ginger detoxification tissue culture device according to claim 1, wherein the whole structure of the stirring tank (21) is a cylindrical two-section structure, the stirring tank (21) and the telescopic sleeve (42) are fixedly connected through a limiting sliding groove (39), two groups of sucker supporting feet (20) are fixedly arranged at the bottom of the sealed shell (17), the sealed shell (17) and the box body base (3) are fixedly connected through the sucker supporting feet (20), the fixed bottom box (6) and the culture box (9) are fixedly connected through a combined bottom plate (30), and the separation clamping plate (8) and the movable clamping rod (4) are elastically connected through a spring pin (38).
3. The tissue culture device for ginger detoxification according to claim 1, wherein a water collection tank (11) is fixedly installed on the outer surface of the upper end of the culture tank (9), two groups of sealing covers (10) are fixedly installed on the outer surface of the front end of the water collection tank (11), a liquid collection hopper (33) is fixedly installed at the bottom of the water collection tank (11), a separation plate is fixedly installed in the middle of the inner surface of the inner side of the water collection tank (11), intercepting plates (35) are movably installed on the inner surface of the water collection tank (11) close to two sides of the separation plate, two groups of second sliding buckles (34) are movably installed on the front portion of the water collection tank (11), the intercepting plates (35) and the second sliding buckles (34) are fixed through welding, a communicating pipe (32) is fixedly installed at the lower end of the liquid collection hopper (33), and a three-way pipe (.
4. The tissue culture device for ginger detoxification according to claim 1, wherein two groups of fixed chucks (29) are movably mounted on the outer surface of the upper end of the combined bottom plate (30), the combined bottom plate (30) and the fixed bottom box (6) are fixedly butted through the fixed chucks (29), two groups of first sliding buckles (28) are movably mounted on the front portion of the combined bottom plate (30), the fixed chucks (29) and the first sliding buckles (28) are fixedly butted through clamping grooves, four groups of fixed bottom bolts (27) are fixedly sleeved on the bottom of the combined bottom plate (30), the outer surfaces of two sides of the separation clamping plate (8) are of an arc structure, and the butting clamping grooves are formed in one side of the fixed chucks (29).
5. The tissue culture device for ginger detoxification according to claim 1, wherein a side rotating flip cover (25) is movably mounted on one side of the sealed housing (17) close to the upper portion of the stirring tank (21), the sealed housing (17) and the side rotating flip cover (25) are fixedly butted through a rotating clamping frame (26), a motor (24) is fixedly sleeved on the inner side of the sealed housing (17), a driving rod (23) is fixedly mounted at the lower end of the motor (24), a rotating stirring rod (22) is fixedly mounted at the lower end of the driving rod (23), and the motor (24) and the rotating stirring rod (22) are fixedly butted through the driving rod (23).
6. The tissue culture device for ginger detoxification according to claim 1, wherein two sets of container boxes (16) are fixedly installed on the outer surface of the upper end of the box base (3) close to the other side of the sealing shell (17), a cleaning basin (18) is fixedly installed on the outer surface of one side of the box base (3), a connecting pipe (15) is fixedly installed on the cleaning basin (18), a drainage pipe head (19) is fixedly installed on the outer surface of the lower end of the connecting pipe (15), and a spray head is fixedly installed on the outer surface of the upper end of the connecting pipe (15).
7. The tissue culture device for ginger detoxification according to claim 1, wherein an induced draft fan (7) is fixedly installed on the outer surface of the side edge of the incubator (9), a plurality of groups of ventilation grooves are formed in the inner side of the upper portion of the incubator (9) in a penetrating mode, two groups of lamp tubes are fixedly installed inside the incubator (9), a fixed vertical plate (14) is fixedly installed at the upper end of the box body base (3), a rotating top plate (12) is movably installed on the upper portion of the fixed vertical plate (14), the rotating top plate (12) and the fixed vertical plate (14) are fixedly connected through hinges in a butt joint mode, and a fluorescent lamp tube (13) is fixedly installed on the outer surface of the bottom of the rotating top plate (12.
8. The tissue culture device for ginger detoxification according to claim 1, wherein four groups of supporting legs (2) are fixedly installed on the outer surface of the bottom of the box base (3), sliding casters (1) are fixedly installed on one side, close to the supporting legs (2), of the bottom of the box base (3), a rotating support plate (5) is movably installed on the outer surface of the front end of the box base (3), and the box base (3) and the rotating support plate (5) are supported and fixed through foot stands.
9. The ginger detoxification tissue culture device according to claim 1, wherein the inner surface of the upper part of the fixed bottom box (6) is provided with a planting groove, and the inner surface of the inner side of the three-way pipe (36) is provided with a circular notch.
10. The cultivation method of the ginger detoxification tissue culture device according to claim 1, which comprises the following specific operation steps:
firstly, a limiting sliding chute (39) between a stirring tank (21) and a telescopic sleeve (42) is utilized to pull the stirring tank (21) downwards, the telescopic sleeve (42) is drawn out, a side rotary turnover cover (25) is turned over through a rotary clamping frame (26), the upper part of the stirring tank (21) is opened, a culture medium raw material is poured into the stirring tank (21), the side rotary turnover cover (25) is closed, a motor (24) is started, the motor (24) drives a rotary stirring rod (22) to rotate through a driving rod (23), two groups of sealing covers (10) of a water collecting tank (11) are opened, and the mixed culture medium and water are poured into the water collecting tank (11) respectively;
placing the ginger blocks in a container box (16), covering fine sand soaked by carbendazim in the container box, placing the ginger blocks in a fixed bottom box (6) for culturing, inserting a separation clamping plate (8) in the fixed bottom box (6) according to the sizes of the ginger blocks, pushing a movable clamping rod (4) to clamp the fixed separation clamping plate (8) by utilizing a spring pin (38), covering soil on the fixed bottom box (6), pushing a second sliding buckle (34) of a water collecting tank (11), and opening a cut-off plate (35) to enable a culture medium and a water body to enter a communicating pipe (32) through a liquid collecting hopper (33), and discharging the culture medium and the water body from a circular notch of a three-way pipe (36) to the fixed bottom box (6);
and step three, when the buds of the ginger blocks grow to 2 cm, taking out the ginger blocks in the fixed bottom box (6), sterilizing the ginger blocks for seven minutes through mercuric chloride, placing the ginger blocks in the incubator (9) again, modulating the light intensity of a lamp tube in the incubator (9) to 4000 lux, adjusting the illumination time to 12 hours every day, after the cultivation is finished, placing the ginger blocks in the cleaning basin (18), and washing the ginger blocks with sterile water through a spray head of the connecting tube (15).
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