CN110720412A - In-vitro hatching device and method for shrimp fertilized eggs - Google Patents
In-vitro hatching device and method for shrimp fertilized eggs Download PDFInfo
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- CN110720412A CN110720412A CN201911221428.8A CN201911221428A CN110720412A CN 110720412 A CN110720412 A CN 110720412A CN 201911221428 A CN201911221428 A CN 201911221428A CN 110720412 A CN110720412 A CN 110720412A
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- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01K—ANIMAL HUSBANDRY; CARE OF BIRDS, FISHES, INSECTS; FISHING; REARING OR BREEDING ANIMALS, NOT OTHERWISE PROVIDED FOR; NEW BREEDS OF ANIMALS
- A01K61/00—Culture of aquatic animals
- A01K61/10—Culture of aquatic animals of fish
- A01K61/17—Hatching, e.g. incubators
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- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01K—ANIMAL HUSBANDRY; CARE OF BIRDS, FISHES, INSECTS; FISHING; REARING OR BREEDING ANIMALS, NOT OTHERWISE PROVIDED FOR; NEW BREEDS OF ANIMALS
- A01K63/00—Receptacles for live fish, e.g. aquaria; Terraria
- A01K63/04—Arrangements for treating water specially adapted to receptacles for live fish
-
- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01K—ANIMAL HUSBANDRY; CARE OF BIRDS, FISHES, INSECTS; FISHING; REARING OR BREEDING ANIMALS, NOT OTHERWISE PROVIDED FOR; NEW BREEDS OF ANIMALS
- A01K63/00—Receptacles for live fish, e.g. aquaria; Terraria
- A01K63/04—Arrangements for treating water specially adapted to receptacles for live fish
- A01K63/042—Introducing gases into the water, e.g. aerators, air pumps
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- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01K—ANIMAL HUSBANDRY; CARE OF BIRDS, FISHES, INSECTS; FISHING; REARING OR BREEDING ANIMALS, NOT OTHERWISE PROVIDED FOR; NEW BREEDS OF ANIMALS
- A01K63/00—Receptacles for live fish, e.g. aquaria; Terraria
- A01K63/04—Arrangements for treating water specially adapted to receptacles for live fish
- A01K63/045—Filters for aquaria
-
- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01K—ANIMAL HUSBANDRY; CARE OF BIRDS, FISHES, INSECTS; FISHING; REARING OR BREEDING ANIMALS, NOT OTHERWISE PROVIDED FOR; NEW BREEDS OF ANIMALS
- A01K63/00—Receptacles for live fish, e.g. aquaria; Terraria
- A01K63/04—Arrangements for treating water specially adapted to receptacles for live fish
- A01K63/047—Liquid pumps for aquaria
-
- Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
- Y02—TECHNOLOGIES OR APPLICATIONS FOR MITIGATION OR ADAPTATION AGAINST CLIMATE CHANGE
- Y02A—TECHNOLOGIES FOR ADAPTATION TO CLIMATE CHANGE
- Y02A40/00—Adaptation technologies in agriculture, forestry, livestock or agroalimentary production
- Y02A40/80—Adaptation technologies in agriculture, forestry, livestock or agroalimentary production in fisheries management
- Y02A40/81—Aquaculture, e.g. of fish
Abstract
The invention relates to a shrimp fertilized egg in-vitro hatching device and a hatching method, wherein fertilized egg blocks are stripped from the abdomen of a parent shrimp; placing the separated fertilized egg mass on a 20-40 mesh screen, adding into a mixture of protease and carbohydrase, performing oscillatory enzymolysis, and aerating to crack the fertilized egg mass to form fertilized egg monomer or single chain; then disinfecting, rinsing for 2 times in disinfected seawater, and adopting a shrimp oosperm in-vitro hatching device to hatch oosperm monomers: uniformly spreading fertilized egg monomers subjected to enzymolysis, disinfection and cleaning on a silk tray, and then placing the fertilized egg monomers in a culture container; form high dissolved oxygen water after making the dissolved oxygen volume promote to water aeration in the water storage container, the water pump lasts to be gone into culture vessel with high dissolved oxygen water pump, and the water change volume is about 1/3 every day in the control culture vessel to the zygote is in mild high dissolved oxygen rivers, and the zygote develops into shrimp larva, lures the lamp through cultivating the shrimp and collects the larva.
Description
Technical Field
The invention relates to an in-vitro hatching device and a hatching method for shrimp fertilized eggs.
Background
The requirements of the precious shrimps on the environment are high, the death of shrimps is easy to occur in the process of artificial fishing or culture, and particularly after the death of the brooding parent shrimps, fertilized eggs need to be separated as soon as possible and then put into a sterilized water body with high dissolved oxygen and proper water temperature for incubation and culture; the mode that prior art adopted to the hatching pond aeration usually ensures that the dissolved oxygen volume in the hatching pond satisfies the cultivation demand, however, the embryonated egg receives the bubble striking easily and damages or breaks among the aeration process to in order to ensure water quality, prior art needs often to carry out a large amount of changes to the water in the hatching pond, and it is more to take water, and causes the sudden change of water microenvironment easily.
Disclosure of Invention
The invention aims to solve the problems and provides an in vitro hatching device for shrimp fertilized eggs, which is realized by the following technical scheme:
an in-vitro hatching device for shrimp fertilized eggs comprises a water storage container, a backwater container and a culture container, wherein a plurality of layers of bolting silk trays are arranged in the culture container, the water storage container is connected with the culture container through a water supply pipeline, and the water supply pipeline is connected with a first water pump; the water storage container is connected with a first aeration pipe, and the first aeration pipe is connected with an external first fan; the outer side of the side wall of the upper part of the culture container is connected with an overflow pipe higher than the bolting silk, and the orifice of the overflow pipe is positioned above the opening of the backwater container; a shrimp luring lamp is arranged on the inner wall of one side of the upper part of the culture container, which is far away from the overflow pipe; the water return container is connected with the water storage container through a water return pipeline, and a second water pump is arranged in the water return pipeline.
The in-vitro shrimp fertilized egg hatching device is further designed in that a pair of slide rails is vertically arranged in the culture container, a culture frame is vertically slidably arranged in the slide rails, and the bolting silk tray is arranged in the culture frame.
The in-vitro shrimp fertilized egg hatching device is further designed in that a limiting block which is abutted against the lower end of the culture frame is arranged at the lower part of the sliding rail.
The in-vitro shrimp fertilized egg hatching device is further designed in that a homogenizing plate positioned below the bolting silk is arranged at the lower part of the culture container, a plurality of water outlet holes are distributed on the plate surface at one side of the homogenizing plate, a water inlet hole is formed in the plate surface at the other side of the homogenizing plate, and the water inlet hole is connected with the water supply pipeline, so that the water supply pipeline is connected with the culture container.
The shrimp fertilized egg in-vitro hatching device is further designed in that a filter screen is arranged on the upper part of the water return container, an ultraviolet lamp is arranged on the lower part of the water return container, the water return container is also connected with a second aeration pipe, and the second aeration pipe is connected with a second fan.
The in-vitro shrimp fertilized egg hatching device is further designed in that the filter screen is obliquely arranged at the upper part of the water return container, and the lower side of the filter screen penetrates out of the side wall of the water return container.
The in-vitro hatching device for the shrimp fertilized eggs is further designed in that a dissolved oxygen probe and a thermometer are arranged in the water storage container; and a temperature adjusting device is arranged at the bottom of the water storage container.
A shrimp oosperm in-vitro hatching method comprises the following steps:
stripping the fertilized egg mass from the abdomen of the parent shrimp;
placing the separated fertilized egg masses into a protease and carbohydrase mixed solution, and aerating to crack the fertilized egg masses to form a plurality of fertilized egg monomers;
adopting the in-vitro hatching device for the shrimp oosperm to hatch the oosperm monomer: collecting fertilized egg monomers by utilizing the bolting silk, and then placing the bolting silk in the culture container; aerating water in the water storage container to enable the dissolved oxygen to be increased to form high dissolved oxygen water, and continuously pumping the high dissolved oxygen water into the culture container by the first water pump, so that fertilized eggs are in a gentle high dissolved oxygen water flow and develop into shrimp larvae; and after overflowing, the water in the culture container enters a water returning container, and is pumped into the water storage container through the second water pump after being filtered, sterilized and aerated in the water returning container.
The in vitro hatching method of the shrimp fertilized eggs is further designed as follows: the mixed solution of the protease and the carbohydrase comprises 30000U/L trypsin, 20000U/L neutral protease, 22000U/L alkaline protease and 15000U/L saccharifying enzyme.
The invention has the beneficial effects that:
by aerating in the water storage container, the water with high dissolved oxygen can smoothly enter the culture container, thereby avoiding the rupture of fertilized eggs due to aeration and improving the hatching rate; in addition, by arranging the water return container, the operation of filtering and the like is directly carried out on the water overflowing from the culture container in the water return container, so that the waste of water resources is reduced; through set up the slide rail in the culture container for the bolting-silk that carries the embryonated egg can steadily get into the aquatic, and the slide rail can make the position relatively fixed of bolting-silk in the water, thereby the cooperation is with the homogenization board of fixed setting in culture container lower part, makes the water dissolved oxygen volume that the embryonated egg that is located on the bolting-silk locates even and stable.
Drawings
Fig. 1 is a schematic structural diagram of an embodiment of the present invention.
FIG. 2 is a schematic view of the structure of the leveling plate.
Detailed Description
The invention is further illustrated by the following figures and examples in conjunction with the description:
the in-vitro hatching device for shrimp fertilized eggs as shown in fig. 1 comprises a water storage container 1, a water return container 2 and a culture container 3, wherein a plurality of bolting silk 4 are arranged in the culture container, the water storage container is connected with the culture container through a water supply pipeline, and the water supply pipeline is connected with a first water pump 5; the water storage container is connected with a first aeration pipe 6 which is connected with an external first fan (not shown in the figure); the outer side of the upper side wall of the culture container 3 is connected with an overflow pipe 31 higher than the bolting silk, and the orifice of the overflow pipe is positioned above the opening of the backwater container; the water return container 2 is connected with the water storage container 1 through a water return pipeline, and a second water pump 7 is arranged in the water return pipeline. The upper part of the culture container 3 is far away from the inner wall of one side of the overflow pipe 31, namely the inner wall of the left side in the embodiment of figure 1, and a shrimp luring lamp 32 is arranged on the inner wall of the left side, so that the larvae gather to the left side of the culture container and can not flow out along with water at the position of the overflow pipe 31.
Example in application, the whole fertilized egg mass was separated from the abdomen of a parent shrimp or a parent shrimp within 30 minutes of death, and then the fertilized egg mass was placed in a protease and carbohydrase mixed solution (trypsin 30000U/L, neutral protease 20000U/L, alkaline protease 22000U/L, and carbohydrase 15000U/L.) and aerated therein, shaking was performed to lyse the fertilized egg mass to form a plurality of fertilized egg monomers, and the fertilized egg monomers were collected with bolting silk (collected once for 5 minutes) and then placed in a culture vessel; after the water in the water storage container is aerated to ensure that the dissolved oxygen is increased by 6.5-7.0mg/L, the first water pump continuously pumps the water with high dissolved oxygen into the culture container, so that the fertilized eggs are in a gentle high dissolved oxygen water flow to promote the fertilized eggs to develop into shrimp larvae; and after overflowing, water in the culture container enters a water returning container, and is pumped into the water storage container through a second water pump after being filtered, sterilized and aerated in the water returning container.
Specifically, a pair of slide rails 8 are vertically provided in the culture container, a culture rack 9 is vertically slidably provided in the slide rails, and the bolting silk 4 is placed in the culture rack. Correspondingly, the lower part of the slide rail is provided with a limiting block 81 which is abutted against the lower end of the culture rack. The upper part of the culture shelf 9 is provided with a lifting rod 91, which is convenient for an operator to lift the culture shelf.
Referring to fig. 2, a homogenizing plate 10 is disposed below the bolting silk at the lower part of the culture container, a plurality of water outlet holes 101 are distributed on one side of the homogenizing plate, and a water inlet hole 102 is formed on the other side of the homogenizing plate and connected with the water supply pipeline, so that the water supply pipeline is connected with the culture container.
Through set up the slide rail in the culture container for the bolting-silk that carries the embryonated egg can steadily get into the aquatic, and the slide rail can make the position relatively fixed of bolting-silk in the water, thereby the cooperation is with the homogenization board of fixed setting in culture container lower part, makes the water dissolved oxygen volume that the embryonated egg that is located on the bolting-silk locates even and stable.
A filter screen 21 is arranged at the upper part of the water return container, an ultraviolet lamp 22 is arranged at the lower part of the water return container, the water return container is also connected with a second aeration pipe 23, and the second aeration pipe is connected with a second fan (not shown in the figure). The air outlet of the second aeration pipe is positioned below the water inlet of the water return pipeline.
The filter screen 21 is obliquely arranged at the upper part of the water return container, and the lower side of the filter screen penetrates through the side wall of the water return container. The filth of interception on the filter screen can be in the lower one side gathering of filter screen, the clearance of being convenient for to because the delivery port of overflow pipe is located the top of the higher one side of filter screen, thereby the water that gets into the return water container can not strike the filth, avoids the filth to pass the filter screen under washing.
The water storage container 1 is internally provided with an oxygen dissolving probe 11 and a thermometer; the bottom of the water storage container is provided with a temperature adjusting device 12, wherein a thermal resistor and a heat exchanger are arranged in the temperature adjusting device. The temperature and the dissolved oxygen amount of water entering the culture container can be controlled more accurately, so that the outlet water temperature of the water storage container is 22-26 ℃ to promote the hatching of fertilized eggs.
Claims (10)
1. The in-vitro hatching device for shrimp fertilized eggs is characterized by comprising a water storage container, a water return container and a culture container, wherein a plurality of layers of bolting silk trays are arranged in the culture container, the water storage container is connected with the culture container through a water supply pipeline, and the water supply pipeline is connected with a first water pump; the water storage container is connected with a first aeration pipe, and the first aeration pipe is connected with an external first fan; the outer side of the side wall of the upper part of the culture container is connected with an overflow pipe higher than the bolting silk tray, and the orifice of the overflow pipe is positioned above the opening of the backwater container; the larva inducing lamp is positioned above the culture container, and the overflow pipe is opposite to the larva inducing lamp; a shrimp luring lamp is arranged on the inner wall of one side of the upper part of the culture container, which is far away from the overflow pipe; the water return container is connected with the water storage container through a water return pipeline, and a second water pump is arranged in the water return pipeline.
2. The in-vitro hatching device for shrimp fertilized eggs according to claim 1, wherein a pair of slide rails are vertically arranged in the culture container, a culture rack is vertically slidably arranged in the slide rails, and the bolting silk tray is placed in the culture rack.
3. The in-vitro hatching device for shrimp fertilized eggs according to claim 2, wherein a limiting block which is abutted against the lower end of the culture rack is arranged at the lower part of the slide rail.
4. The in vitro hatching device for fertilized eggs of shrimps as claimed in claim 2, wherein the lower part of the culture container is provided with a homogenizing plate under the bolting silk, one side of the homogenizing plate is distributed with a plurality of water outlets, the other side of the homogenizing plate is provided with water inlets, and the water inlets are connected with the water supply pipeline, so that the water supply pipeline is connected with the culture container.
5. The in-vitro hatching device for shrimp fertilized eggs according to claim 1, wherein a filter screen is arranged at the upper part of the water return container, an ultraviolet lamp is arranged at the lower part of the water return container, and the water return container is further connected with a second aeration pipe which is connected with a second fan.
6. The in-vitro hatching device for fertilized eggs of shrimps as claimed in claim 5, wherein the filter screen is obliquely arranged at the upper part of the water return container, and the lower side of the filter screen penetrates out of the side wall of the water return container.
7. The in vitro hatching apparatus for fertilized shrimp eggs according to claim 1, wherein a dissolved oxygen probe and a thermometer are disposed in the water storage container; and a temperature adjusting device is arranged at the bottom of the water storage container.
8. A shrimp oosperm in-vitro hatching method is characterized by comprising the following steps:
stripping the fertilized egg mass from the abdomen of the parent shrimp;
placing the separated fertilized egg mass on a 20-40 mesh screen, adding into a mixture of protease and carbohydrase, performing oscillatory enzymolysis, and aerating to crack the fertilized egg mass to form fertilized egg monomer or single chain; then sterilizing in potassium permanganate solution, and rinsing in sterilized seawater for 2 times;
hatching fertilized egg monomers by adopting the in-vitro hatching device for shrimp fertilized eggs as claimed in claim 1: uniformly laying fertilized egg monomers subjected to enzymolysis, disinfection and cleaning on a special 60-mesh silk tray with the diameter of 20-40cm, and then placing the fertilized egg monomers in a culture container; (ii) a Aerating water in the water storage container to enable the dissolved oxygen to be improved to form high dissolved oxygen water, continuously pumping the high dissolved oxygen water into the culture container by the first water pump, adjusting the flow of the pump, controlling the daily water change amount in the culture container to be 1/3, so that fertilized eggs are in a gentle high dissolved oxygen water flow, the fertilized eggs develop into shrimp larvae, and collecting the larvae through a lamp induction device at the top of the culture container; and after overflowing, the water in the culture container enters a water returning container, and is pumped into the water storage container through the second water pump after being filtered, sterilized and aerated in the water returning container.
9. The method for hatching a fertilized egg of shrimp in vitro according to claim 8, wherein: the protease and carbohydrase are composed of a mixed solution of the protease and the carbohydrase, namely 30000U/L of trypsin, 20000U/L of neutral protease, 22000U/L of alkaline protease and 15000U/L of saccharifying enzyme.
10. The method for hatching a fertilized egg of shrimp in vitro according to claim 8, wherein: the dissolved oxygen of the effluent in the reservoir is 6.5-7.0mg/L, and the effluent temperature is 22-26 ℃.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201911221428.8A CN110720412A (en) | 2019-12-03 | 2019-12-03 | In-vitro hatching device and method for shrimp fertilized eggs |
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| Application Number | Priority Date | Filing Date | Title |
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| CN201911221428.8A CN110720412A (en) | 2019-12-03 | 2019-12-03 | In-vitro hatching device and method for shrimp fertilized eggs |
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| CN110720412A true CN110720412A (en) | 2020-01-24 |
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| CN201911221428.8A Pending CN110720412A (en) | 2019-12-03 | 2019-12-03 | In-vitro hatching device and method for shrimp fertilized eggs |
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Cited By (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN111328742A (en) * | 2020-03-03 | 2020-06-26 | 天津农学院 | Method for improving hatching rate of in-vitro culture of embryos of young shrimps with saw teeth |
| CN111557256A (en) * | 2020-06-16 | 2020-08-21 | 杭州强维智能技术有限公司 | Aquaculture is with hatching bucket with roe disinfection and water purification function |
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Cited By (4)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN111328742A (en) * | 2020-03-03 | 2020-06-26 | 天津农学院 | Method for improving hatching rate of in-vitro culture of embryos of young shrimps with saw teeth |
| CN111328742B (en) * | 2020-03-03 | 2021-12-14 | 天津农学院 | Method for improving hatching rate of in-vitro culture of embryos of young shrimps with saw teeth |
| CN111557256A (en) * | 2020-06-16 | 2020-08-21 | 杭州强维智能技术有限公司 | Aquaculture is with hatching bucket with roe disinfection and water purification function |
| CN111557256B (en) * | 2020-06-16 | 2022-01-18 | 安源生物科技(上海)有限公司 | Aquaculture is with hatching bucket with roe disinfection and water purification function |
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