CN110716011B - Device for detecting atmospheric microorganisms - Google Patents

Device for detecting atmospheric microorganisms Download PDF

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Publication number
CN110716011B
CN110716011B CN201910806644.2A CN201910806644A CN110716011B CN 110716011 B CN110716011 B CN 110716011B CN 201910806644 A CN201910806644 A CN 201910806644A CN 110716011 B CN110716011 B CN 110716011B
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pipe
sealing tank
wall
air
fixedly connected
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CN110716011A (en
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马万征
汪建飞
谢越
肖新
李孝良
蔡永兵
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Deweiyi (Beijing) Technology Co.,Ltd.
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Anhui University of Science and Technology
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    • GPHYSICS
    • G01MEASURING; TESTING
    • G01NINVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N33/00Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
    • G01N33/0004Gaseous mixtures, e.g. polluted air
    • AHUMAN NECESSITIES
    • A61MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61LMETHODS OR APPARATUS FOR STERILISING MATERIALS OR OBJECTS IN GENERAL; DISINFECTION, STERILISATION OR DEODORISATION OF AIR; CHEMICAL ASPECTS OF BANDAGES, DRESSINGS, ABSORBENT PADS OR SURGICAL ARTICLES; MATERIALS FOR BANDAGES, DRESSINGS, ABSORBENT PADS OR SURGICAL ARTICLES
    • A61L2/00Methods or apparatus for disinfecting or sterilising materials or objects other than foodstuffs or contact lenses; Accessories therefor
    • A61L2/02Methods or apparatus for disinfecting or sterilising materials or objects other than foodstuffs or contact lenses; Accessories therefor using physical phenomena
    • A61L2/08Radiation
    • GPHYSICS
    • G01MEASURING; TESTING
    • G01NINVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N33/00Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
    • G01N33/0004Gaseous mixtures, e.g. polluted air
    • G01N33/0009General constructional details of gas analysers, e.g. portable test equipment

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  • Chemical & Material Sciences (AREA)
  • Health & Medical Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • General Health & Medical Sciences (AREA)
  • Analytical Chemistry (AREA)
  • Immunology (AREA)
  • Physics & Mathematics (AREA)
  • Food Science & Technology (AREA)
  • Biochemistry (AREA)
  • Combustion & Propulsion (AREA)
  • General Physics & Mathematics (AREA)
  • Medicinal Chemistry (AREA)
  • Pathology (AREA)
  • Epidemiology (AREA)
  • Animal Behavior & Ethology (AREA)
  • Public Health (AREA)
  • Veterinary Medicine (AREA)
  • Apparatus Associated With Microorganisms And Enzymes (AREA)

Abstract

The invention discloses a device for detecting atmospheric microorganisms in the technical field of environmental monitoring, which comprises a sealing tank, a supporting plate and an electric push rod, wherein the sealing tank and the electric push rod are respectively and fixedly connected to the right and left sides of the top of the supporting plate vertically, a liquid discharge pipe with a manual valve is arranged at the lower part of the right side of the outer wall of the sealing tank in a penetrating and communicating manner, a gas collecting device is horizontally welded at the top of the electric push rod, a first exhaust fan is fixedly connected to the upper part of the left side of the outer wall of the sealing tank through a horizontal welding bracket, an air inlet of the first exhaust fan is connected with an air outlet of the gas collecting device through a hose, DTT liquid is placed in the sealing tank, when monitoring is not needed, an inert gas recycling device is used for releasing inert gas to fill the sealing tank to prevent oxidation, when the air is needed to be used, the air is injected, meanwhile, the inert gas is recycled, the change rate of a PH (potential of hydrogen) meter is observed for digital display, and the air quality is judged, the device is convenient to operate and use in multiple environments.

Description

Device for detecting atmospheric microorganisms
Technical Field
The invention relates to the technical field of environmental monitoring, in particular to a device for detecting atmospheric microorganisms.
Background
The oxidation potential of atmospheric particulates is commonly used for representing the influence of particulates on human health, a Dithiothreitol (DTT) method is a method for determining the oxidation potential of particulates developed in recent years, and a common reducing agent for dithiothreitol has antioxidation, has similar effect compared with mercaptoethanol, but has much smaller pungent smell and much lower toxicity than the mercaptoethanol; when the concentration of DTT is 7 times lower than that of mercaptoethanol, the two effects are similar, and the DTT is poor in stability because the DTT is easily oxidized by air; but the service life of the product can be prolonged by freezing and storing the product or processing the product in inert gas, and the effective reducibility of DTT is reduced along with the reduction of pH value due to the lower nucleophilicity of the protonated sulfur; the present invention is also commonly used for reducing disulfide bonds in proteins, and can be used for preventing intramolecular or intermolecular disulfide bonds of proteins formed between cysteines in proteins, and by using the disulfide bonds to react with proteins of microorganisms in the atmosphere, the content of the microorganisms in the atmosphere can be determined by detecting a change in PH, and further the air quality is known.
Disclosure of Invention
The invention aims to provide a device for measuring the microbial oxidation potential of atmospheric particulates, and aims to solve the problem that a device for monitoring the atmospheric environment by utilizing DDT to react with protein of microbes in the atmosphere and judging the content of the microbes in the atmosphere by detecting the change of pH value is urgently needed to be designed.
In order to achieve the purpose, the invention provides the following technical scheme: a device for detecting atmospheric microorganisms comprises a sealing tank, a supporting plate and an electric push rod, wherein the sealing tank and the electric push rod are respectively and fixedly connected to the right side and the left side of the top of the supporting plate in a perpendicular mode, a liquid discharge pipe with a manual valve is arranged on the lower portion of the right side of the outer wall of the sealing tank in a penetrating mode, a gas collecting device is horizontally welded on the top of the electric push rod, a first exhaust fan is fixedly connected to the upper portion of the left side of the outer wall of the sealing tank through a horizontal welding support, an air inlet of the first exhaust fan is connected with an air outlet of the gas collecting device through a hose, a first connecting pipe with the manual valve is arranged between an air outlet of the first exhaust fan and the sealing tank in a communicating mode, a first digital display flow valve is arranged on the first connecting pipe, a low-speed motor is fixed to the top of the sealing tank in an inverted mode, a stirrer is fixedly connected to the bottom power output end of the low-speed motor, a PH digital display is arranged on the lower portion of the left side of the sealing tank in a communicating mode, the upper part of the right side of the outer wall of the sealing tank is communicated with an inert gas recycling device;
receive gas device and include the thick pipe of right-hand member welding gradual change pipe, the outer wall lower part right side of thick pipe is fixed with the jam through the screw thread through-hole, the sealed spiro union of left end of thick pipe has the inner tube, the inner wall fixedly connected with LED sterilization lamp pearl of inner tube, the right-hand member of inner tube is provided with the baffle through the sealed laminating of torsional spring and fixed axle.
Furthermore, a timer is embedded and fixed on the outer wall of the sealing tank.
Further, handles are embedded in the centers of the two sides of the upper surface of the supporting plate.
Furthermore, a mesh pipe with a sponge sleeve glued on the inner wall is arranged on the outer side of the shell of the low-speed motor.
Further, the inert gas cyclic utilization device includes the rectangle box body that the opening is forward, the inner chamber bottom fixedly connected with second air exhauster of rectangle box body, the inner wall right side upper portion of rectangle box body communicates with each other and is fixed with the Y type pipe that the branch end has the solenoid valve, the lower part branch end of Y type pipe is connected with the air outlet of second air exhauster, the air intake of second air exhauster and the upper portion branch end homogeneous phase through-weld of Y type pipe have the second connecting pipe that runs through and extend to the seal pot inner chamber, the main head of Y type pipe is provided with the digital flow valve of second, the outer end spiro union of Y type pipe has elastic air bag.
Furthermore, the outer wall of the elastic air bag is fixedly connected with anti-puncture cloth.
Compared with the prior art, the invention has the beneficial effects that: according to the invention, DTT liquid is placed in the sealed tank, when monitoring is not required, the inert gas recycling device is used for releasing inert gas to fill the sealed tank, so that oxidation is prevented, when the device is required to be used, air is injected, meanwhile, the inert gas is recovered, the change rate of the PH meter reading is observed and displayed, and the air quality is judged.
Drawings
In order to more clearly illustrate the technical solutions of the embodiments of the present invention, the drawings used in the description of the embodiments will be briefly introduced below, and it is obvious that the drawings in the following description are only some embodiments of the present invention, and it is obvious for those skilled in the art that other drawings can be obtained according to the drawings without creative efforts.
FIG. 1 is a schematic structural view of the present invention;
FIG. 2 is a schematic structural view of the gas collecting device in FIG. 1;
fig. 3 is a schematic structural view of the inert gas recycling device in fig. 1.
In the drawings, the components represented by the respective reference numerals are listed below:
1-a sealed tank, 2-a supporting plate, 3-a liquid discharge pipe, 4-an electric push rod, 5-an air collecting device, 6-a first exhaust fan, 7-a hose, 8-a first connecting pipe, 9-a first digital display flow valve, 10-a low-speed motor, 11-a stirrer, 12-a digital display PH meter, 13-an inert gas recycling device, 50-a thick pipe, 51-a plug, 52-an inner pipe, 53-a baffle, 54-an LED sterilizing lamp bead, 130-a rectangular box body, 131-a second exhaust fan, 132-a Y-shaped pipe, 133-a second connecting pipe, 134-a second digital display flow valve and 135-an elastic air bag.
Detailed Description
The technical solutions in the embodiments of the present invention will be clearly and completely described below with reference to the drawings in the embodiments of the present invention, and it is obvious that the described embodiments are only a part of the embodiments of the present invention, and not all of the embodiments. All other embodiments, which can be derived by a person skilled in the art from the embodiments given herein without making any creative effort, shall fall within the protection scope of the present invention.
In the description of the present invention, it is to be understood that the terms "upper", "lower", "front", "rear", "left", "right", "top", "bottom", "inner", "outer", and the like, indicate orientations or positional relationships based on the orientations or positional relationships shown in the drawings, are merely for convenience in describing the present invention and simplifying the description, and do not indicate or imply that the device or element being referred to must have a particular orientation, be constructed and operated in a particular orientation, and thus, should not be construed as limiting the present invention.
Referring to fig. 1-3, the present invention provides a technical solution: a device for detecting atmospheric microorganisms comprises a sealing tank 1, a supporting plate 2 and an electric push rod 4, wherein the sealing tank 1 and the electric push rod 4 are respectively vertically and fixedly connected to the right and left sides of the top of the supporting plate 2, a liquid discharge pipe 3 with a manual valve is arranged at the lower part of the right side of the outer wall of the sealing tank 1 in a penetrating and communicating manner, a gas receiving device 5 is horizontally welded at the top of the electric push rod 4, a first exhaust fan 6 is fixedly connected to the upper part of the left side of the outer wall of the sealing tank 1 through a horizontal welding support, an air inlet of the first exhaust fan 6 is connected with an air outlet of the gas receiving device 5 through a hose 7, a first connecting pipe 8 with a manual valve is arranged between an air outlet of the first exhaust fan 6 and the sealing tank 1 in a communicating manner, a first digital display flow valve 9 is arranged on the first connecting pipe 8, a low-speed motor 10 is fixed on the top of the sealing tank 1 in an inverted manner, and a stirrer 11 is fixedly connected to the power output end of the bottom of the low-speed motor 10, the lower part of the left side of the sealing tank 1 is communicated with a digital display PH meter 12, and the upper part of the right side of the outer wall of the sealing tank 1 is communicated with an inert gas recycling device 13;
receive gas arrangement 5 and include the thick pipe 50 of right-hand member welding gradual change pipe, the outer wall lower part right side of thick pipe 50 is fixed with the jam 51 through the screw thread through-hole, the sealed spiro union in left end of thick pipe 50 has inner tube 52, the inner wall fixedly connected with LED sterilization lamp pearl 54 of inner tube 52, the right-hand member of inner tube 52 is provided with baffle 53 through torsional spring and the sealed laminating of fixed axle, LED sterilization lamp pearl 54 lights a period earlier before operating means, kill the inner wall and breed the microorganism, the improvement degree of accuracy, first air exhauster 6 draws in the air, low-speed motor 10 and 11 stirs of agitator, close the manual valve on the first connecting pipe 8, first digital display flow valve 9 record air flow, and then estimate the little biological concentration trend of atmosphere, electric putter 4 drives and receives gas arrangement 5 and reciprocates, be convenient for gather the data of co-altitude not.
Wherein, the outer wall of seal pot 1 inlays and is fixed with the timer, is convenient for record PH rate of change.
The handles are embedded in the centers of the two sides of the upper surface of the supporting plate 2, so that the carrying is convenient.
The outer side of the shell of the low-speed motor 10 is provided with a mesh pipe, the inner wall of which is glued with a sponge sleeve, so that the low-speed motor 10 is protected, and meanwhile, the heat dissipation is not influenced.
The inert gas recycling device 13 comprises a rectangular box body 130 with a forward opening, a second exhaust fan 131 is fixedly connected to the bottom of an inner cavity of the rectangular box body 130, a Y-shaped pipe 132 with a branch end provided with a solenoid valve is fixedly communicated with the upper part of the right side of the inner wall of the rectangular box body 130, the lower branch end of the Y-shaped pipe 132 is connected with an air outlet of the second exhaust fan 131, a second connecting pipe 133 penetrating and extending to the inner cavity of the seal tank 1 is welded to an air inlet of the second exhaust fan 131 and the upper branch end of the Y-shaped pipe 132, a second digital flow valve 134 is arranged at the main end of the Y-shaped pipe 132, an elastic air bag 135 is screwed to the outer end of the Y-shaped pipe 132, the second digital flow valve 134 records the flow of outflow gas and backflow gas, the upper solenoid valve is opened when inert gas is injected, the elastic air bag 135 is squeezed, the plug 51 is opened, an inert gas detector is placed at the outlet, and the inert gas selects argon gas with a density higher than that of air, when argon gas is detected, the plug 51 is closed, and when argon gas is recovered, the solenoid valve at the lower part is opened.
The outer wall of the elastic air bag 135 is fixedly connected with anti-puncturing cloth, so that the service life is prolonged.
In the description herein, references to the description of "one embodiment," "an example," "a specific example" or the like are intended to mean that a particular feature, structure, material, or characteristic described in connection with the embodiment or example is included in at least one embodiment or example of the invention. In this specification, the schematic representations of the terms used above do not necessarily refer to the same embodiment or example. Furthermore, the particular features, structures, materials, or characteristics described may be combined in any suitable manner in any one or more embodiments or examples.
The preferred embodiments of the invention disclosed above are intended to be illustrative only. The preferred embodiments are not intended to be exhaustive or to limit the invention to the precise embodiments disclosed. Obviously, many modifications and variations are possible in light of the above teaching. The embodiments were chosen and described in order to best explain the principles of the invention and the practical application, to thereby enable others skilled in the art to best utilize the invention. The invention is limited only by the claims and their full scope and equivalents.

Claims (5)

1. The utility model provides a detect atmospheric microorganisms's device, includes seal pot (1), layer board (2) and electric putter (4), its characterized in that: the sealing tank (1) and the electric push rod (4) are respectively vertically and fixedly connected to the right and left sides of the top of the supporting plate (2), a liquid discharge pipe (3) with a manual valve is arranged on the lower portion of the right side of the outer wall of the sealing tank (1) in a penetrating and communicating mode, a gas collecting device (5) is horizontally welded on the top of the electric push rod (4), a first exhaust fan (6) is fixedly connected to the upper portion of the left side of the outer wall of the sealing tank (1) through a horizontal welding support, an air inlet of the first exhaust fan (6) is connected with an air outlet of the gas collecting device (5) through a hose (7), a first connecting pipe (8) with a manual valve is arranged between an air outlet of the first exhaust fan (6) and the sealing tank (1) in a communicating mode, a first digital display flow valve (9) is arranged on the first connecting pipe (8), and a low-speed motor (10) is fixed to the top of the sealing tank (1) in an inverted mode, a stirrer (11) is fixedly connected with the power output end at the bottom of the low-speed motor (10), the lower part of the left side of the sealing tank (1) is communicated with a digital display PH meter (12), and the upper part of the right side of the outer wall of the sealing tank (1) is communicated with an inert gas recycling device (13);
the gas collecting device (5) comprises a thick pipe (50) with a gradually-changed pipe welded at the right end, a plug (51) is fixed on the right side of the lower portion of the outer wall of the thick pipe (50) through a threaded through hole, an inner pipe (52) is connected to the left end of the thick pipe (50) in a sealing and threaded mode, an LED sterilizing lamp bead (54) is fixedly connected to the inner wall of the inner pipe (52), and a baffle (53) is arranged at the right end of the inner pipe (52) in a sealing and laminating mode through a torsion spring and a fixing shaft;
inert gas cyclic utilization device (13) are including opening rectangle box body (130) forward, the inner chamber bottom fixedly connected with second air exhauster (131) of rectangle box body (130), the inner wall right side upper portion of rectangle box body (130) communicates with each other and is fixed with Y type pipe (132) that the branch end has the solenoid valve, the lower part branch end of Y type pipe (132) is connected with the air outlet of second air exhauster (131), the air intake of second air exhauster (131) and the upper portion branch end homogeneous phase through-weld of Y type pipe (132) have run through and extend to second connecting pipe (133) of seal pot (1) inner chamber, the main aspects of Y type pipe (132) are provided with second digital flow valve (134), the outer end spiro union elasticity gasbag (135) of Y type pipe (132).
2. An apparatus for detecting atmospheric microorganisms as defined in claim 1, wherein: and a timer is embedded and fixed on the outer wall of the sealing tank (1).
3. An apparatus for detecting atmospheric microorganisms as defined in claim 1, wherein: handles are embedded in the centers of the two sides of the upper surface of the supporting plate (2).
4. An apparatus for detecting atmospheric microorganisms as defined in claim 1, wherein: and a mesh pipe with a sponge sleeve glued on the inner wall is arranged on the outer side of the shell of the low-speed motor (10).
5. An apparatus for detecting atmospheric microorganisms as defined in claim 1, wherein: the outer wall of the elastic air bag (135) is fixedly connected with anti-puncture cloth.
CN201910806644.2A 2019-08-29 2019-08-29 Device for detecting atmospheric microorganisms Active CN110716011B (en)

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CN110716011B true CN110716011B (en) 2022-03-11

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Citations (7)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
DE19903986A1 (en) * 1999-02-02 2000-08-10 Friedrich Boelsing Reductive dehalogenation of halohydrocarbons under mild conditions, e.g. for removing toxic chloroaromatic compounds from waste oil or soil, using reducing metal in presence of amine
CN204314304U (en) * 2015-01-08 2015-05-06 河南农业大学 A kind of device directly measuring hydrogenase or carbon monoxide dehydrogenase activity in anaerobe
CN205379753U (en) * 2016-01-15 2016-07-13 江苏海事职业技术学院 Second -hand -cigarette absorbs and processing apparatus
CN106574226A (en) * 2014-08-20 2017-04-19 3M创新有限公司 Self-contained anaerobic culture device for sulfate-reducing microorganisms
CN108872033A (en) * 2018-05-24 2018-11-23 香港理工大学深圳研究院 The heterogeneous reaction activity measurement device and method of gaseous pollutant in atmospheric environment
CN109596472A (en) * 2018-12-26 2019-04-09 复旦大学 Carbonaceous concentration systems in a kind of measurement Atmospheric particulates
CN110106062A (en) * 2019-05-05 2019-08-09 上海交通大学 A kind of device of modularity control ORP reactor

Family Cites Families (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
FR2939445B1 (en) * 2008-12-10 2013-05-03 Biomerieux Sa AUTOMATED LYSE SYSTEM OF MICROORGANISMS PRESENT IN SAMPLE, NUCLEIC ACID EXTRACTION AND PURIFICATION OF MICROORGANISMS FOR ANALYSIS

Patent Citations (7)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
DE19903986A1 (en) * 1999-02-02 2000-08-10 Friedrich Boelsing Reductive dehalogenation of halohydrocarbons under mild conditions, e.g. for removing toxic chloroaromatic compounds from waste oil or soil, using reducing metal in presence of amine
CN106574226A (en) * 2014-08-20 2017-04-19 3M创新有限公司 Self-contained anaerobic culture device for sulfate-reducing microorganisms
CN204314304U (en) * 2015-01-08 2015-05-06 河南农业大学 A kind of device directly measuring hydrogenase or carbon monoxide dehydrogenase activity in anaerobe
CN205379753U (en) * 2016-01-15 2016-07-13 江苏海事职业技术学院 Second -hand -cigarette absorbs and processing apparatus
CN108872033A (en) * 2018-05-24 2018-11-23 香港理工大学深圳研究院 The heterogeneous reaction activity measurement device and method of gaseous pollutant in atmospheric environment
CN109596472A (en) * 2018-12-26 2019-04-09 复旦大学 Carbonaceous concentration systems in a kind of measurement Atmospheric particulates
CN110106062A (en) * 2019-05-05 2019-08-09 上海交通大学 A kind of device of modularity control ORP reactor

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