CN110106062A - A kind of device of modularity control ORP reactor - Google Patents
A kind of device of modularity control ORP reactor Download PDFInfo
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- CN110106062A CN110106062A CN201910367421.0A CN201910367421A CN110106062A CN 110106062 A CN110106062 A CN 110106062A CN 201910367421 A CN201910367421 A CN 201910367421A CN 110106062 A CN110106062 A CN 110106062A
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- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
- C12M23/00—Constructional details, e.g. recesses, hinges
- C12M23/02—Form or structure of the vessel
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- C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
- C12M23/00—Constructional details, e.g. recesses, hinges
- C12M23/38—Caps; Covers; Plugs; Pouring means
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- C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
- C12M29/00—Means for introduction, extraction or recirculation of materials, e.g. pumps
- C12M29/04—Filters; Permeable or porous membranes or plates, e.g. dialysis
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- C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
- C12M29/00—Means for introduction, extraction or recirculation of materials, e.g. pumps
- C12M29/06—Nozzles; Sprayers; Spargers; Diffusers
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- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
- C12M41/00—Means for regulation, monitoring, measurement or control, e.g. flow regulation
- C12M41/28—Means for regulation, monitoring, measurement or control, e.g. flow regulation of redox potential
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- C12M41/00—Means for regulation, monitoring, measurement or control, e.g. flow regulation
- C12M41/44—Means for regulation, monitoring, measurement or control, e.g. flow regulation of volume or liquid level
Abstract
The invention discloses a kind of devices of modularity control ORP reactor, are related to microbial fermentation and microorganism electro synthesis field, including ventilation/reagent delivery systems, electric energy input system, fermentation system and ORP regulator control system；ORP regulator control system is used to monitor ORP and the control ventilation, reagent and voltage input of fermentation system；Ventilation/reagent delivery systems gas output end and reagent output end pass through transmission pipeline respectively and connect with cover, across cover and are fixed on cover；Electric energy input system is fixed on cover, and is connect by conducting wire with ORP regulator control system；Cover is detachable, and ventilation/reagent delivery systems are switched rapidly with electric energy input system.The present invention controls three kinds the apparatus module of ORP, so as to select different control methods to be controlled for different fermentation systems, promotes the yield and efficiency of microbial fermentation production product, provides a new thinking for fermentation industry.
Description
Technical field
The present invention relates to microbial fermentation and microorganism electro synthesis fields more particularly to a kind of modularity control ORP to react
The device of device.
Background technique
The product of microbial fermentation plays critically important role in people's daily life, such as ethyl alcohol, acetic acid, 1,3-
Propylene glycol etc..But for chemical method synthesis, the low output of microbial fermentation is at high cost to limit it in the market
Competitiveness.Therefore the yield and yield that improve microbial fermentation are one of the emphasis of biological field research.
The more commonly used method of fermentation industry is to lead to suitable gas into fermentation system at present, to promote microorganism
Growth and fermenting property.But the excessive growth for leading to the microorganism that oxygen will lead to part amphimicrobian is suppressed, to reduce
The production of product；The excessively few respiration that may then reduce part microorganism of oxygen-supply quantity, limits the generation of internal energy, together
Sample can inhibit the growth and fermentation of microorganism.Therefore, to different microorganisms, the oxygen-supply quantity optimized is also different, need
It is monitored in real time.In addition, in the case where logical pure oxygen not can be effectively controlled fermentation system ORP, it can also be to fermentation
Air or nitrogen are passed through in system.
Similarly, some redox reagents, oxidant such as potassium ferricyanide, potassium bichromate, peroxide are added into fermentation system
Change benzoic acid etc., reducing agent such as dithiothreitol (DTT), stannous chloride, sodium borohydride etc. also influences whether growth and the hair of microorganism
Ferment.Being monitored to the concentration of system internal oxidition go back original reagent is also the required emphasis paid close attention to.
Microorganism electro synthesis is field emerging in recent years, by the inputing power into fermentation system, so that fermentation is thin
Born of the same parents receive a certain amount of electronics and change the yield and yield of target product to influence intracellular metabolic pathway.It is fermenting
Industrially there is good application prospect.But the same is the electric energy input of excessive or deficient amount will not all make microorganism reach
To optimal fermenting property.Therefore need to monitor in real time the technology of response of the cell to electric energy at present, thus complete to electric energy into
The control of amount promotes the income of fermentation while avoiding waste of energy.
Oxidation-reduction potential (Oxidation-Reduction Potential, hereinafter referred to as ORP) and it is able to reflect cell
Redox state, thus the case where indirectly reflecting the precellular growth of mesh and fermentation.
Therefore, those skilled in the art is dedicated to developing a kind of device of modularity control ORP reactor, can be with
The technology of three of the above control microbial fermentation performance be combined with each other, and by reflecting the ORP of cell in real time, is able to know that at present
Whether cell needs and needs the input of how many gas, redox reagent, electric energy, so that control strategy is timely adjusted,
The fermenting property for improving cell, improves the yield and yield of target product.
Summary of the invention
In view of the above drawbacks of the prior art, the technical problem to be solved by the present invention is in order to find it is a kind of can will lead to
Gas method is mended redox reagent method, electric energy input method and is combined, selectively, efficiently to the process of microbial fermentation into
The ORP method of row monitoring, can efficiently control the process entirely fermented.
To achieve the above object, the present invention provides a kind of devices of modularity control ORP reactor, including ventilation/examination
Agent Transmission system, electric energy input system, fermentation system and ORP regulator control system；
The fermentation system at least has there are two cover, the ventilation/reagent delivery systems and the electric energy input system
It is joined respectively on the different covers, the cover is detachable；
The gas output end and reagent output end of the ventilation/reagent delivery systems pass through transmission pipeline and the tank respectively
Lid connection, across the cover and is fixed on the cover；Flow control portion is provided in the ventilation/reagent delivery systems
Part；
The electric energy input system is fixed on the cover, and is connect by conducting wire with the ORP regulator control system；
The ORP regulator control system includes ORP controller and the ORP electrode for monitoring oxidation-reduction potential；The ORP electricity
Pole passes through the cover and is inserted into the fermentation system；The flow control component is connected by conducting wire and the ORP controller
It connects；The ORP controller controls the output of gas and reagent by controlling the flow control component.
Further, card slot and multiple through-holes are provided on the fermentation system cover, the card slot is for described in fixation
Electric energy input system, the through-hole for fixing the transmission pipeline, the conducting wire and the ORP electrode, the transmission pipeline,
The conducting wire and the ORP electrode are each passed through the different through-holes.
Further, sensor and reference electrode are integrated on the ORP electrode.
Further, the ventilation/reagent delivery systems include ventilation subsystem and reagent subsystem；The ventilation subsystem
System includes at least one gas source, and the gas output end of the gas source is connected by the flow control component with the transmission pipeline
To a through-hole of the cover；The reagent subsystem includes the feed supplement bottle that at least one is equipped with reagent, the feed supplement bottle
A through-hole being connected on the cover by the flow control component and the transmission pipeline of reagent output end on, one
A corresponding through-hole of the feed supplement bottle.
Further, if the gas source quantity is greater than one, also the flow control component and the through-hole it
Between transmission pipeline on be provided with multiple-way valve, be used for transmission the gas of multiple gas sources.
It further, further include humidifier, buffer unit and gas filter membrane in the ventilation subsystem；The humidification dress
It sets, the buffer unit and the gas filter membrane are set to transfer tube between the gas flow control unit and the through-hole
On road；When the ventilation subsystem includes the multiple-way valve, the humidifier, the buffer unit and the gas filter membrane
It is set on the transmission pipeline between the multiple-way valve and the through-hole.
Further, the ventilation subsystem includes three gas sources, respectively two gas cylinders and an air pump；The multi-pass
Valve is four-way valve；The gas cylinder is respectively provided with two kinds of different gases, is provided with electronics air valve on each gas cylinder；It is described
Air pump is for extracting air；The electronics air valve, the air pump are connect with the four-way valve by transmission pipeline respectively, and described four
Port valve is connect with a through-hole on the cover by transmission pipeline；The electronics air valve, the air pump pass through conducting wire respectively
It is connect with the ORP controller.
Further, the reagent subsystem includes two feed supplement bottles, is respectively provided with Oxidizing and Reducing Agents；Described in one
Feed supplement bottle passes through a peristaltic pump and transmission pipeline respectively and connect with a through-hole on the cover；The peristaltic pump is by leading
Line is connect with the ORP controller.
Further, the electric energy input module includes voltage input system and system for partition wall；The voltage input system
Including voltage-stablizer, cathode carbon cloth, anode carbon cloth；The system for partition wall includes two pieces of plastic plates and proton exchange membrane；The plastics
Plate posts and fixes on the card slot of the cover, and the fermentation system is divided into cathode chamber and anode chamber；The proton is handed over
Film is changed to be clipped among the plastic plate；The cathode carbon cloth is located at the cathode chamber, and the anode carbon cloth is located at the anode chamber,
The voltage-stablizer is located at outside the fermentation system, and the cathode carbon cloth is connect with the cathode of the voltage-stablizer by conducting wire, described
Anode carbon cloth is connect with the anode of the voltage-stablizer by conducting wire.
Further, the anode chamber is mounted with reproducibility reagent solution；The cathode chamber is mounted with culture Institute of Micro-biology
The culture solution needed.
The working principle of the device of modularity control ORP reactor provided by the invention:
Oxidation-reduction potential (ORP) is the ability of solution receiving and losing electrons, is used as unit with millivolt (millivolt, mV).
ORP value height represent solution obtain electronic capability it is stronger, be in the state of oxidation；The low ability increasing for representing solution and providing electronics of ORP value
It by force, is reducing condition.Extracellular ORP value can be used as the reflection of electronics transfer and redox equilibrium net result intracellular.Thin
In the metabolic process of born of the same parents, since the co-factor of part enzyme is NAD (P) H or NAD (P)+, with this part, enzyme participates in cell
Metabolic response in, the ratio regular meeting of co-factor intracellular changes therewith, and intracellular redox state it is most of by
The redox state for can change cell that these co-factors are determined, therefore are metabolized.So by monitoring extracellular ORP
Numerical value, can indirectly reflect the whole metabolic condition of cell interior, to know the precellular growth conditions of mesh and metabolism production
Situation can be used as the whole index for carrying out situation of monitoring fermentation.
Other than the ORP to fermentation system is monitored, can also it control it.It is controlled by the ORP to system
System, can make intracellular redox state run-off the straight, i.e., so that the ratio of co-factor changes.This influences whether generation
It thanks to the reaction that the enzyme for needing these co-factors in approach and these enzymes participate in, the metabolism network of cell is finally made to change,
To which the growth of cell and the production of fermented product can also change.The currently used redox shape for being used to change cell interior
The method of state has: mending redox reagent method, venting method and electric energy input method.
Mending redox reagent method is the reagent that addition has Strong oxdiative reproducibility into fermentation system, these reagents can be straight
The intracellular redox state of the change connect, to influence the growth and production of cell.However excessive redox reagent
The growth of cell can be caused to inhibit, reduce the generation of metabolism product；Owe the reagent of amount then will not oxidation to cell interior also
Original state impacts, and does not achieve the effect that control.And since ORP can reflect growth conditions and the generation of cell on the whole
Thank to the condition of production, so the concentration for capableing of real-time control redox reagent by monitoring ORP, avoids excessive and invalid control
System.
Venting method is by supplementing suitable oxygen into fermentation system, on the one hand, oxygen is as standard oxidationreduction potential
High substance (+820mV) can significantly affect the oxidation-reduction potential in solution, and on the other hand, the breathing that oxygen enhances cell is made
With the energy for improving cell interior generates, to promote the growth of cell and change metabolism flow direction.However, excessive or deficient amount
Gas negative impact can be caused to microbial cell, therefore, by monitor ORP variation can adjust gas in real time
Input, control it, so that the input of gas is optimal.In addition, fermentation cannot be efficiently controlled in oxygen
In the case of the ORP of system, be optionally passed through air or nitrogen thereto, the two can also by monitor the variation of ORP come
Adjustment input in real time.
Electric energy input rule is by inputting electronics to cell interior, and portion can be deposited electronics in the form of NADH in the cell
With the input of electronics, the quantity of NADH also increases therewith, and the ratio of co-factor intracellular can also change, so that cell
Interior redox state run-off the straight influences the growth of cell and the metabolism of inside.Specifically, by utilizing system for partition wall will
Fermentation system is divided into cathode chamber and anode chamber, and cathode chamber is responsible for loading the culture solution of microorganism, carries out the culture of microorganism, and positive
Pole room then loads the solution of the reagent of tool reducing property.Have proton exchange membrane among system for partition wall, with it is hydrionic transfer to
Circuit in being connected to.Apply voltage at this both ends, electronics is transmitted to cell interior by carbon cloth electrode, changes the redox of cell
State, while it being back to voltage-stablizer from anode chamber, complete the connection of external circuit.
By replacing the fermentation system cover, to realize the switching between described two big modules；ORP regulation system
System is by control peristaltic pump and air pump, to realize the switching between ventilation subsystem and the big system of reagent subsystem two.
Beneficial effects of the present invention:
1, ORP is a kind of index that can reflect cell growth and the metabolism condition of production on the whole.Pass through the prison to ORP
Control, can more effectively control the process entirely fermented.
2, the control that ORP is carried out using venting method, not will cause too big burden to the growth of cell itself, in addition the party
Cost is relatively low for method, environmental protection, will not be to subsequent fermentation production process.
3, using the control for mending redox reagent method progress ORP, it can rapidly, significantly change the oxidation of cell also
Original state is able to carry out efficient control.
4, venting method and benefit redox reagent method are integrated in reagent/gas delivery system, when a certain kind therein
When method cannot efficiently control ORP, it another kind method can be used to be controlled, allow the operator to selectively carry out
ORP control.
5, peristaltic pump/air pump can accelerate gas and redox reagent transmits in a device, effectively improve reagent/gas
The efficiency of Transmission system progress ORP control.
6, the control that electric energy input method carries out ORP can be directly changed the ratio of internal co-factor, thus more efficiently
Ground, the redox state for directly changing cell itself.
6, fermentation system can be divided into anode chamber and cathode chamber by system for partition wall, and by proton exchange membrane institute between two Room
Connection, facilitates control of the electric energy input method to fermentation system ORP.
7, system for partition wall and voltage input system are combined into a module, so that electric energy input method controls ORP module
Change, the convenient reconstruct to installation for fermenting.And it can be complementary to one another with reagent/gas delivery system module, each leisure ORP control of completion
Defect in system expands the range of ORP control.
It is described further below with reference to technical effect of the attached drawing to design of the invention, specific structure and generation, with
It is fully understood from the purpose of the present invention, feature and effect.
Detailed description of the invention
Fig. 1 is the system principle schematic diagram of a preferred embodiment of the invention；
Fig. 2 is ventilation/reagent delivery systems schematic diagram of a preferred embodiment of the invention；
Fig. 3 is the top view of fermentation system cover；
Fig. 4 is the electric energy input system schematic diagram of a preferred embodiment of the invention.
Wherein: 1- fermentation system, 2- cover, 3-ORP electrode, 4-ORP controller, 5- oxidising agent feed supplement bottle, 6- reduction
Reagent feed supplement bottle, 7- transmission pipeline, 8- peristaltic pump, 9- conducting wire, 10- air pump, 11- nitrogen cylinder, 12- electronics air valve, 13- four-way
Valve, 14- humidifier, 15- buffer unit, 16- gas filter membrane, 17- oxygen cylinder, 18- cathode carbon cloth, 19- proton exchange membrane,
20- voltage-stablizer, 21- anode carbon cloth, 22- plastic plate, 23- first through hole, the second through-hole of 24-, 25- card slot, 26- third through-hole,
27- fourth hole.
Specific embodiment
A preferred embodiment of the present invention is introduced below with reference to Figure of description, keeps its technology contents more clear and just
In understanding.The present invention can be emerged from by many various forms of embodiments, and protection scope of the present invention not only limits
The embodiment that Yu Wenzhong is mentioned.
In the accompanying drawings, the identical component of structure is indicated with same numbers label, everywhere the similar component of structure or function with
Like numeral label indicates.The size and thickness of each component shown in the drawings are to be arbitrarily shown, and there is no limit by the present invention
The size and thickness of each component.Apparent in order to make to illustrate, some places suitably exaggerate the thickness of component in attached drawing.
As shown in Figure 1, the device of modularity control ORP reactor of the invention includes ventilation/reagent delivery systems, electric energy
Input system, fermentation system and ORP regulator control system；Fermentation system at least has there are two cover 2, ventilation/reagent delivery systems and
Electric energy input system is joined respectively on different covers 2, and cover 2 is detachable.
ORP regulator control system includes ORP controller 4 and the ORP electrode 3 for monitoring oxidation-reduction potential, and ORP electrode 3 is worn
Cover 2 is crossed, is inserted into fermentation system 1.ORP electrode 3 is connect with ORP controller 4 by conducting wire 9.Biography is incorporated on ORP electrode 3
Sensor and reference electrode.Sensor is made of inert metal (such as platinum, rhodium, iridium, gold), and reference electrode is Ag/AgCl.
Ventilation/reagent delivery systems gas output end and reagent output end pass through transmission pipeline 7 respectively and cover 2 connects
It connects, and is fixed on cover 2；It is provided with flow control component in ventilation/reagent delivery systems, passes through conducting wire and ORP controller 4
Connection, ORP controller 4 control the output of gas and reagent by controlling the flow control component.Thus form ventilation/
Reagent delivery module.
Electric energy input system is fixed on cover 2, and is connect by conducting wire with ORP regulator control system；It is defeated that electric energy is consequently formed
Enter module.
Cover 2 is set as removably, by dismantling different cover, thus realize ventilation/reagent delivery module and
Rapid switching between electric energy input module.
As shown in Fig. 2, ventilation/reagent delivery systems include ventilation subsystem and reagent subsystem.Ventilation subsystem is used for
Realize venting method control fermentation.In the present embodiment, three gas sources, respectively oxygen cylinder 17, nitrogen cylinder 11 and air pump are provided with
10.Oxygen cylinder 17 is used to provide oxygen to fermentation system 1, and nitrogen cylinder 11 is used to provide nitrogen to fermentation system 1, and air pump 10 is used for
Air is provided to fermentation system 1.In order to easily control the flow of gas and select the gas type for needing to input, in oxygen cylinder
17, be respectively provided with electronics air valve 12 on the gas outlet of nitrogen cylinder 11, by electronics air valve 12, air pump 10 respectively by conducting wire with
ORP controller 4 is connected.Meanwhile electronics air valve 12, air pump 10 are also connected to four-way valve 13 by transmission pipeline 7, then four-way valve
13 are connected on cover 2 by transmission pipeline 7.In order to improve the humidity of gas, prevent air-flow excessive and filtering gas in
Dust has also set gradually humidifier 14, buffer unit 15 and gas filter membrane 16 in the gas circuit of four-way valve 13 to cover 2.
Reagent subsystem is realized for providing different types of reductant-oxidant into fermentation system 1 and mends the control of redox reagent method
The fermentation of microorganism.In the present embodiment, it is provided with two feed supplement bottles, respectively oxidising agent feed supplement bottle 5 and go back original reagent feed supplement
Bottle 6.Oxidising agent feed supplement bottle 5 and go back original reagent feed supplement bottle 6 are connected on cover 2 by respective peristaltic pump 8 respectively, mutually it
Between connected by transmission pipeline 7.Meanwhile peristaltic pump 8 is also connected on ORP controller 4 by conducting wire 9.
On cover 2, ORP electrode 3 passes through cover 2 and is inserted into fermentation system 1.Incorporated on ORP electrode 3 sensor and
The signal measured the case where can monitoring the growth of microorganism and be metabolized product, and is transferred to by reference electrode by conducting wire 9
ORP controller 4.After ORP controller 4 is fed back, according to preset threshold value, by controlling peristaltic pump 8, electronics air valve 12, gas
The start and stop of pump 10 and revolving speed, the input of adjustment redox reagent and air, oxygen, nitrogen, so that the ORP of fermentation system 1 is controlled
System is in Near Threshold.By the way that the reagent output end of the gas output end for subsystem of ventilating and reagent subsystem is integrated in cover 2
On, so that ventilation/reagent delivery systems modularization, with the disassembly of cover 2, to realize that ventilation/reagent delivery module is torn open
It unloads.
It is worth noting that, in ventilation/reagent delivery systems, the gas source of subsystem of ventilating can according to actual needs into
Row selection, oxygen cylinder 17, nitrogen cylinder 11 and the air pump 10 being not limited in the present embodiment.It, can be with if only needing a gas source
It is not provided with four-way valve 13.If necessary to which multiple gas sources are arranged, suitable multiple-way valve can be selected according to gas source quantity, being used for will not
In the gas transport of same type to fermentation system 1.In order to control gas flow, it is suitable to be selected according to the type of gas source carrier
Flow control component.If selecting gas cylinder, electronics air valve 12 can be selected and be used as flow control component；If selection input is empty
Gas, air are not needed gas cylinder loading, the input and flow control of air can be completed using air pump 10.In reagent subsystem
In, the quantity of feed supplement bottle can also be selected according to actual needs.For the ease of controlling the stream of oxidising agent and go back original reagent respectively
An individual peristaltic pump 8 is arranged to each feed supplement bottle in amount；Meanwhile on cover 2, the reagent output end of different feed supplement bottles makes
With different through-holes, for reagent to be separated.
As shown in figure 3, being provided with first through hole 23, the second through-hole 24, third through-hole 26 and fourth hole 27 on cover 2.
In order to be applicable in cover 2 between different modules, it is also provided with the card slot 25 for stationary electric input system on cover 2, incites somebody to action
Hereinafter it is described in detail.It needs to input gas, Oxidizing and Reducing Agents in fermentation system 1 in the present embodiment, also need
It is inserted into ORP electrode 3, and Oxidizing and Reducing Agents cannot mix before entering fermentation system 1, therefore, first through hole 23, the
Two through-holes 24, third through-hole 26 and fourth hole 27 are respectively for insertion into gas output end, oxidant output end, reducing agent output
End and ORP electrode 3.Number of openings on cover 2 can be configured according to actual needs.
As shown in figure 4, electric energy input system includes system for partition wall and voltage input system.System for partition wall includes plastic plate 22
With proton exchange membrane 19；Voltage input system includes voltage-stablizer 20, cathode carbon cloth 18, anode carbon cloth 21.Plastic plate 22 is two pieces,
It is provided with clamp device on its edge, two pieces of plastic plates 22 are bonded, and is fixed on the card slot 25 of cover 2.In fitting
Among two pieces of plastic plates 22, it is fixed with proton exchange membrane 19.Fermentation system 1 is divided into two chambers by system for partition wall, respectively yin
Pole room and anode chamber.In cathode chamber, it is provided with cathode carbon cloth 18；In anode chamber, it is provided with anode carbon cloth 21；Cathode carbon cloth
18 are connect by conducting wire 9 with the cathode of voltage-stablizer 20；Anode carbon cloth 21 is connect by conducting wire 9 with the anode of voltage-stablizer 20.Cathode
Carbon cloth 18 and anode carbon cloth 21 are all connect by AB glue with conducting wire 9, and voltage-stablizer 20 is arranged outside fermentation system 1, pass through conducting wire 9
It is connect with ORP controller 4.AB glue under room temperature (25 DEG C) can quick solidification, cured glue film is transparent, hardness is high, shock resistance and
The characteristics such as shearing force is strong, high to the adhesive strength of a variety of materials, and weatherability is good.The effect of AB glue mainly prevents conducting wire and fermentation
Liquid contact.The conducting wire 9 of the conducting wire 9 and jointed anode carbon cloth 21 that connect cathode carbon cloth 18 is each passed through the different through-holes on cover 2
It is connected to voltage-stablizer 20.Electric energy input system is fixed on cover 2 by system for partition wall as a result, forms dismountable module.
The culture solution that microorganism is loaded in cathode chamber, carries out the culture of microorganism；The built-in carrier in anode chamber has reducing property
Reagent solution.The centre of plastic plate 22 is provided with cavity, and proton exchange membrane 19 is enabled to be exposed to cathode chamber and anode chamber
Solution in, proton exchange membrane 19 be used for hydrionic transfer, thus be connected in circuit.Proton exchange membrane 19 is Du Pont
117nafion film.Apply voltage to cathode carbon cloth 18 and anode carbon cloth 21 by voltage-stablizer 20, electronics is transmitted by carbon cloth electrode
To cell interior, change the redox state of cell, while being back to voltage-stablizer 20 from anode chamber, completes the company of external circuit
It is logical.
ORP electrode 3 passes through in the through-hole insertion fermentation system 1 on cover 2, is connect by conducting wire 9 with ORP controller 4.
The signal measured is transferred to ORP controller 4 by ORP electrode 3, and ORP controller 4 is after being fed back, according to pre-set threshold
Value adjusts the input of electric energy, so that the ORP of fermentation system 1 is controlled by controlling the start and stop of voltage-stablizer 20 and the output valve of voltage
In Near Threshold.
The application method of the present apparatus is as follows:
When needing to control ORP in fermentation system 1, it is proposed that ventilation/reagent delivery systems are first selected, because this
A system can satisfy the ORP demand for control of most of fermentation system substantially.Ventilation/reagent delivery systems construction method
It has been described above, which is not described herein again.In this system, can by ORP controller 4 mend redox reagent method,
It is switched between venting method.It is recommended that first selection venting method is tested, because this method is at low cost, and environment will not be made
At pollution.The threshold value that ORP control is set on ORP controller 4 can bring into operation.
For part bacterial strain, venting method may not be able to significantly change the redox state of cell, cannot reach obvious
ORP control effect, at this moment can be switched to by ORP controller 4 mend redox reagent method.It needs exist for trying in oxidation in advance
Oxidising agent and go back original reagent are loaded respectively in agent feed supplement bottle 5 and go back original reagent feed supplement bottle 6.Oxidising agent suggestion selects iron cyaniding
Potassium, go back original reagent suggestion select dithiothreitol (DTT), and the two is respectively provided with strong oxidizing property and reproducibility, and cost is relatively low.Switching
After control method, the air pump 10 and electronics air valve 12 for being connected to outside air are not rerunned, connection oxidising agent feed supplement bottle 5 and reduction
The peristaltic pump 8 of reagent feed supplement bottle 6 starts turning.Similarly, it after setting ORP control threshold, can bring into operation.
Ventilation/reagent delivery systems are by changing the ORP numerical value in environment, to indirectly change the oxidation of cell also
Original state, thus for cell itself ORP control efficiency relative to directly to cell input electronics electric energy input method for
It is slightly lower.In addition, electric energy input method control ORP can significantly alter the ratio of the co-factor of cell, thus to intracellular
Most of metabolic fluxes re-directed so that cell generates new metabolism product.So working as ventilation/reagent delivery systems pair
When the ORP control effect of fermentation system is weaker, or when microorganism being needed to generate new metabolism product, it may be considered that use electric energy
Input system.The construction method of electric energy input system, which hereinbefore has been carried out, to be elaborated.Not homology is loaded by disassembly
The cover 2 of system can rapidly switch to electric energy input system.ORP control threshold need to be arranged in operator in ORP controller 4
Value and the voltage of initial voltage-stablizer output, voltage setting suggest that in -1V, excessive voltage can make in fermentation system between 1V
Microbial culture medium electrolysis water reaction occurs, waste of energy while, can have an impact the physiological status of cell itself.If
After setting, it can bring into operation.ORP controller 4 can control the start and stop of voltage-stablizer 20 and the output of voltage so that system ORP
It is controlled near threshold value.
For traditional electric control, lack ORP electrode and ORP control system, only by voltage-stablizer
The input that one constant value to carry out fermentation system electric energy is set.Therefore, suffering from a problem that is, needs to adjust manually
For the output voltage of whole voltage-stablizer to enable electric control effect to be optimal value, the preparation stage before causing control is longer, right
The subsequent industrial applications of electric control have very big obstacle.The basic reason of the problem be lack a monitor fermentation system into
The index of journey, so as to carry out the adjustment of real-time electric energy input.And in electric energy input system of the invention, due to ORP
The presence of this monitoring index so that the input of voltage need not be maintained at a constant value, and can with fermentation into
Journey changes in real time, when the process of optimization input voltage is omitted, and can guarantee fermentation, the input voltage value energy at current time
The course of fermentation for enough matching current time, ensure that the effect of whole electric control.In addition relative to traditional constant voltage
Input, the consumption of electric energy can be saved by adjusting voltage in real time, improved the utilization efficiency of electric energy, the cost of electric control is reduced, after being
Place mat is laid in continuous industrialization.
The device of modularity control fermentation system oxidation-reduction potential provided by the invention by venting method, mends redox
Three kinds of control method modularizations such as reagent method, electric energy input method, so as to selectively be carried out for different fermentation systems
Control expands the range of ORP control, promotes the yield and efficiency of microbial fermentation production product, provides for fermentation industry
One new thinking.
The preferred embodiment of the present invention has been described in detail above.It should be appreciated that the ordinary skill of this field is without wound
The property made labour, which according to the present invention can conceive, makes many modifications and variations.Therefore, all technician in the art
Pass through the available technology of logical analysis, reasoning, or a limited experiment on the basis of existing technology under this invention's idea
Scheme, all should be within the scope of protection determined by the claims.
Claims (10)
1. a kind of device of modularity control ORP reactor, which is characterized in that inputted including ventilation/reagent delivery systems, electric energy
System, fermentation system and ORP regulator control system；
The fermentation system at least has there are two cover, and the ventilation/reagent delivery systems and the electric energy input system are distinguished
It is bound on the different covers, the cover is detachable；
The gas output end and reagent output end of the ventilation/reagent delivery systems pass through transmission pipeline respectively and the cover connects
It connects, across the cover and is fixed on the cover；Flow control component is provided in the ventilation/reagent delivery systems；
The electric energy input system is fixed on the cover, and is connect by conducting wire with the ORP regulator control system；
The ORP regulator control system includes ORP controller and the ORP electrode for monitoring oxidation-reduction potential；The ORP electrode is worn
It crosses the cover and is inserted into the fermentation system；The flow control component is connect by conducting wire with the ORP controller；Institute
ORP controller is stated by controlling the flow control component to control the output of gas and reagent.
2. the device of modularity control ORP reactor as described in claim 1, which is characterized in that the fermentation system cover
On be provided with card slot and multiple through-holes, the card slot is for fixing the electric energy input system, and the through-hole is for described in fixed
Transmission pipeline, the conducting wire and the ORP electrode, the transmission pipeline, the conducting wire and the ORP electrode are each passed through difference
The through-hole.
3. the device of modularity control ORP reactor as described in claim 1, which is characterized in that integrated on the ORP electrode
Sensor and reference electrode.
4. the device of modularity control ORP reactor as claimed in claim 2, which is characterized in that the ventilation/reagent delivery
System includes ventilation subsystem and reagent subsystem；The ventilation subsystem includes at least one gas source, the gas of the gas source
Output end is connected on a through-hole of the cover by the flow control component and the transmission pipeline；Reagent
System includes feed supplement bottle that at least one is equipped with reagent, the reagent output end of the feed supplement bottle by the flow control component and
The transmission pipeline is connected on a through-hole on the cover, the corresponding through-hole of a feed supplement bottle.
5. the device of modularity control ORP reactor as claimed in claim 4, which is characterized in that if the gas source quantity
When greater than one, it is provided with multiple-way valve on the transmission pipeline also between the flow control component and the through-hole, for passing
The gas of defeated multiple gas sources.
6. the device of modularity control ORP reactor as claimed in claim 5, which is characterized in that in the ventilation subsystem
It further include humidifier, buffer unit and gas filter membrane；The humidifier, the buffer unit and gas filter membrane setting
On transmission pipeline between the gas flow control unit and the through-hole；When the ventilation subsystem includes the multi-pass
When valve, the humidifier, the buffer unit and the gas filter membrane are set between the multiple-way valve and the through-hole
On transmission pipeline.
7. the device of modularity control ORP reactor as claimed in claim 5, which is characterized in that the ventilation subsystem packet
Include three gas sources, respectively two gas cylinders and an air pump；The multiple-way valve is four-way valve；The gas cylinder is respectively provided with different
Two kinds of gases are provided with electronics air valve on each gas cylinder；The air pump is for extracting air；The electronics air valve, institute
It states air pump to connect with the four-way valve by transmission pipeline respectively, a through-hole on the four-way valve and the cover passes through biography
Defeated pipeline connection；The electronics air valve, the air pump pass through conducting wire respectively and connect with the ORP controller.
8. the device of modularity control ORP reactor as claimed in claim 4, which is characterized in that the reagent subsystem packet
Two feed supplement bottles are included, Oxidizing and Reducing Agents is respectively provided with；One feed supplement bottle passes through a peristaltic pump and transfer tube respectively
Road is connect with a through-hole on the cover；The peristaltic pump is connect by conducting wire with the ORP controller.
9. the device of modularity control ORP reactor as claimed in claim 2, which is characterized in that the electric energy input module
Including voltage input system and system for partition wall；The voltage input system includes voltage-stablizer, cathode carbon cloth, anode carbon cloth；It is described
System for partition wall includes two pieces of plastic plates and proton exchange membrane；The plastic plate posts and fixes on the card slot of the cover, will
The fermentation system is divided into cathode chamber and anode chamber；The proton exchange membrane is clipped among the plastic plate；The cathode carbon
Cloth is located at the cathode chamber, and the anode carbon cloth is located at the anode chamber, and the voltage-stablizer is located at outside the fermentation system, described
Cathode carbon cloth is connect with the cathode of the voltage-stablizer by conducting wire, and the anode of the anode carbon cloth and the voltage-stablizer passes through conducting wire
Connection.
10. the device of modularity control ORP reactor as claimed in claim 9, which is characterized in that the anode chamber is mounted with
Reproducibility reagent solution；The cathode chamber is mounted with the culture solution that culture Institute of Micro-biology needs.
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