CN110713931A - Embryo culture dish with automatic imaging and automatic liquid changing functions - Google Patents

Embryo culture dish with automatic imaging and automatic liquid changing functions Download PDF

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Publication number
CN110713931A
CN110713931A CN201911177086.4A CN201911177086A CN110713931A CN 110713931 A CN110713931 A CN 110713931A CN 201911177086 A CN201911177086 A CN 201911177086A CN 110713931 A CN110713931 A CN 110713931A
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CN
China
Prior art keywords
rotating shaft
groove
rectangular groove
bevel gear
rotating
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Withdrawn
Application number
CN201911177086.4A
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Chinese (zh)
Inventor
吴美君
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Yuyao Shengpu Biotechnology Co Ltd
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Yuyao Shengpu Biotechnology Co Ltd
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Priority to CN201911177086.4A priority Critical patent/CN110713931A/en
Publication of CN110713931A publication Critical patent/CN110713931A/en
Withdrawn legal-status Critical Current

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    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12MAPPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
    • C12M23/00Constructional details, e.g. recesses, hinges
    • C12M23/02Form or structure of the vessel
    • C12M23/10Petri dish
    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12MAPPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
    • C12M27/00Means for mixing, agitating or circulating fluids in the vessel
    • C12M27/02Stirrer or mobile mixing elements
    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12MAPPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
    • C12M29/00Means for introduction, extraction or recirculation of materials, e.g. pumps
    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12MAPPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
    • C12M31/00Means for providing, directing, scattering or concentrating light
    • C12M31/10Means for providing, directing, scattering or concentrating light by light emitting elements located inside the reactor, e.g. LED or OLED

Abstract

The invention discloses an embryo culture dish with automatic imaging and automatic liquid changing functions, which comprises a box body, wherein a first rotating cavity is arranged in the box body, an illumination component is arranged in the first rotating cavity, a culture dish is arranged on the lower end wall of the first rotating cavity, automatic liquid changing components are arranged on two sides of the culture dish, a second rotating cavity is arranged below the culture dish, an automatic imaging component is arranged in the second rotating cavity, a third rectangular groove is arranged below the second rotating cavity, a fourth rectangular groove is arranged on the left side of the third rectangular groove, a fifth rotating groove is arranged on the upper end wall of the fourth rectangular groove, a second rectangular groove is arranged on the right side of the third rectangular groove, a fourth rotating groove penetrating through the third rectangular groove is arranged between the fourth rectangular groove and the second rectangular groove, a ninth rotating shaft is arranged in the fourth rotating groove, the left end of the ninth rotating shaft extends into the fourth rectangular groove and is fixedly provided with an eleventh bevel gear, and the right end of the ninth rotating shaft extends into the second rectangular groove and is fixedly provided with a seventh bevel gear.

Description

Embryo culture dish with automatic imaging and automatic liquid changing functions
Technical Field
The invention relates to an embryo culture dish with automatic imaging and automatic liquid changing functions.
Background
In vitro culture of human embryos is performed in culture dishes, and most in vitro fertilization laboratories currently use common embryo culture dishes for in vitro culture of oocytes and embryos. Because of the close morphology of each embryo, embryos cannot be directly labeled. The most insuring method is to prepare a single dish for each embryo and perform individual labeling and culturing, but when the number of embryos is large, a large number of dishes are required, which significantly increases the cost, the operation time and the difficulty. Embryo culture fluid plays a very important role because it provides nutrients required for the development of the embryo during its division. At present, the traditional method for replacing the embryo culture solution is to simply suck the culture solution by an artificial liquid sucker. Need go on after taking out the culture dish from the incubator every time when changing the culture solution, such liquid change process intangible has destroyed stable culture system, has caused certain injury to the embryo of culture in-process.
Disclosure of Invention
Aiming at the technical defects, the invention provides the embryo culture dish with the automatic imaging and automatic liquid changing functions, which can overcome the defects.
The invention relates to an embryo culture dish with automatic imaging and automatic liquid changing functions, which comprises a box body, wherein a first rotating cavity is arranged in the box body, an illumination component is arranged in the first rotating cavity, a culture dish is arranged on the lower end wall of the first rotating cavity, automatic liquid changing components are arranged on two sides of the culture dish, a second rotating cavity is arranged below the culture dish, an automatic imaging component is arranged in the second rotating cavity, a third rectangular groove is arranged below the second rotating cavity, a fourth rectangular groove is arranged on the left side of the third rectangular groove, a fifth rotating groove is arranged on the upper end wall of the fourth rectangular groove, a second rectangular groove is arranged on the right side of the third rectangular groove, a fourth rotating groove penetrating through the third rectangular groove is arranged between the fourth rectangular groove and the second rectangular groove, a ninth rotating shaft is arranged in the fourth rotating groove, the left end of the ninth rotating shaft extends into the fourth rectangular groove and is fixedly provided with an eleventh bevel gear, the right end of the ninth rotating shaft extends into the second rectangular groove and is fixedly provided with a seventh bevel gear, a ninth bevel gear which is in power connection with the automatic imaging component is also fixedly arranged on the ninth rotating shaft in the third rectangular groove, a heating cavity is arranged at the left side of the first rotating cavity, a heating assembly is arranged in the heating cavity, a second transmission groove is arranged at the right side of the heating cavity, a driving mechanism for driving the heating assembly is arranged in the second transmission groove, a first rectangular groove is arranged on the right side of the second transmission groove, a first transmission mechanism is arranged in the first rectangular groove, a first transmission groove is arranged on the right side of the first rectangular groove, and a second transmission mechanism is arranged in the first transmission groove, a first rotary groove penetrating through the first rectangular groove is arranged between the second transmission groove and the first transmission groove, and a third rotary groove communicated with each other is arranged between the first transmission groove and the second rectangular groove.
Preferably, the illumination subassembly includes fixed mounting and is in the fourth pivot of well diapire, fourth pivot lower extreme stretches into in the first rotation chamber and fixed mounting have a fourth band pulley, fixed mounting has the fixed plate in the fourth pivot, end wall fixed mounting has the slide under the fixed plate, but the spout horizontal slip in the slide installs the slider, fixed mounting has the second dead lever in the slider, slider lower extreme fixed mounting has the mounting panel, mounting panel lower extreme fixed mounting has the incandescent lamp, it has the third band pulley still to fix on the slide, the third band pulley passes through second belt power with the fourth band pulley and is connected, fixed mounting has the third dead lever on the third band pulley, the third dead lever with the second dead lever passes through second connecting rod fixed connection.
Preferably, the automatic liquid changing assembly comprises a new culture liquid pool and a waste liquid pool which are positioned at two sides of the culture dish, a liquid inlet pipe is fixedly installed between the right end wall of the new culture liquid pool and the left end wall of the culture dish, a first water pump is fixedly installed in the liquid inlet pipe, a twelfth rotating shaft positioned in the fifth rotating groove is dynamically installed at the lower end of the first water pump, the lower end of the twelfth rotating shaft extends into the fourth rectangular groove and is fixedly provided with a twelfth bevel gear meshed with the eleventh bevel gear, a liquid outlet pipe is fixedly installed between the right end wall of the culture dish and the left end wall of the waste liquid pool, a second water pump is fixedly installed in the liquid outlet pipe, an eighth rotating shaft positioned in the third rotating groove is dynamically installed at the lower end of the second water pump, the lower end of the eighth rotating shaft extends into the second rectangular groove and is fixedly provided with a sixth bevel gear meshed with the seventh bevel gear, a seventh rotating shaft positioned in the third rotating groove is dynamically installed at the upper end of the second water pump, a fifth bevel gear is fixedly installed at the upper end of the seventh rotating shaft and extends into the first transmission groove, and a culture hole for placing embryos is further formed in the culture dish.
Preferably, the automatic imaging assembly comprises a gear sleeve engaged with the ninth bevel gear, a support seat is fixedly mounted on an outer end wall of the gear sleeve, a tenth rotating shaft is rotatably mounted on the support seat through a bearing, a tenth bevel gear is fixedly mounted at the right end of the tenth rotating shaft and extends out of the support seat, a roller is fixedly mounted on the tenth rotating shaft, a track groove is formed in the outer surface of the roller, a first fixing rod is movably mounted in the track groove, a first connecting rod is fixedly mounted on an outer end wall of the first fixing rod, a camera is fixedly mounted on the first connecting rod, a second fixing shaft rotatably connected with the first connecting rod is further fixedly mounted on the support seat, the first connecting rod is fixedly connected with the first fixing rod, a fixing shaft is rotatably mounted in the gear sleeve, and an eighth bevel gear engaged with the tenth bevel gear is fixedly mounted at the upper end of the fixing shaft, and the lower end of the fixed shaft is fixedly provided with a fixed block which is fixed on the lower end wall of the third rectangular groove and is in running fit with the ninth rotating shaft.
Preferably, heating element include with the thirteenth pivot that actuating mechanism power is connected, thirteenth pivot left end stretches into in the heating chamber and fixed mounting have the second gear, be equipped with the heating cabinet in the heating chamber, rotationally install the fourteenth pivot in the heating cabinet, fourteenth pivot right-hand member fixed mounting have with the fluted disc of second gear meshing, fixed mounting has the swivel mount in the fourteenth pivot, fixed mounting has the stirring rod on the swivel mount, inner wall fixed mounting has the heating plate in the heating cabinet, heating cabinet upper end wall fixed mounting has the intake pipe, the intake pipe upper end is equipped with opening gas storage chamber to the left, end wall fixed mounting has the outlet duct under the heating cabinet, the outlet duct end stretches into the left end wall of culture dish.
Preferably, the driving mechanism comprises a second rotating shaft located in the first rotating groove, the left end of the second rotating shaft extends into the air storage cavity and is fixedly provided with a fan, the right end of the second rotating shaft extends into the first rectangular groove and is fixedly provided with a first bevel gear, a first belt wheel is further fixedly arranged in the second transmission groove and is located on the second rotating shaft, a second belt wheel is fixedly arranged in the thirteenth rotating shaft, the right end of the thirteenth rotating shaft extends into the second transmission groove and is fixedly provided with a second belt wheel, and the first belt wheel is in power connection with the second belt wheel through a first belt.
Preferably, the first transmission mechanism includes a motor in power connection with the upper end of the fourth rotating shaft, a second bevel gear engaged with the first bevel gear is fixedly installed in the first rectangular groove on the fourth rotating shaft, a fifth rotating shaft is rotatably installed in the first rotating groove through a bearing, and the left end of the fifth rotating shaft extends into the first rectangular groove and is fixedly installed with a third bevel gear engaged with the second bevel gear.
Preferably, the second transmission mechanism comprises a fourth bevel gear meshed with the fifth bevel gear, a sixth rotating shaft is fixedly mounted at the right end of the fourth bevel gear, a first gear is fixedly mounted at the right end of the sixth rotating shaft, and an incomplete gear meshed with the first gear is fixedly mounted at the right end of the fifth rotating shaft and extends into the first transmission groove.
The beneficial effects are that: when the device is used, the motor can drive the illumination assembly to rotate, the fourth rotating shaft can drive the incandescent lamp to slide left and right by driving the sliding block to slide left and right, and the illumination of the embryos in the culture dish is homogenized due to the continuous rotation and left and right sliding motion of the incandescent lamp, so that each embryo is ensured to have enough illumination in each direction;
when in use, the second rotating shaft can suck air into the air storage cavity through the fan,
the second rotating shaft can also drive the stirring rod to rotate through belt transmission to stir the air entering from the air inlet pipe, so that the air forms vortex airflow, the time of the air in the heating box can be prolonged, and the heating uniformity of the air is improved;
during the use, the motor accessible drive the second water pump can with in surpass the waste liquid of liquid level passes through the drain pipe is carried in the waste liquid pond, simultaneously the power transmission of second water pump is to first water pump, first water pump can with new culture solution in the new culture solution pond is carried in the culture dish, because new culture solution pond can last toward carry new culture solution in the culture dish, can guarantee the nutrient of embryo is sufficient to improve embryo culture's success rate.
Drawings
In order to more clearly illustrate the embodiments of the invention or the technical solutions in the prior art, the drawings used in the description of the embodiments or the prior art will be briefly described below, and it is obvious that the drawings in the following description are only some embodiments of the invention, and it is obvious for those skilled in the art that other drawings can be obtained based on these drawings without creative efforts.
FIG. 1 is a schematic structural diagram of an embodiment of the present invention;
FIG. 2 is a schematic diagram of A in FIG. 1 according to an embodiment of the present invention;
FIG. 3 is an enlarged schematic view of the structure at B in FIG. 1 according to an embodiment of the present invention;
fig. 4 is a top view at the drum of fig. 1 in an embodiment of the present invention.
Detailed Description
All of the features disclosed in this specification, or all of the steps in any method or process so disclosed, may be combined in any combination, except combinations of features and/or steps that are mutually exclusive.
Any feature disclosed in this specification may be replaced by alternative features serving equivalent or similar purposes, unless expressly stated otherwise. That is, unless expressly stated otherwise, each feature is only an example of a generic series of equivalent or similar features.
As shown in fig. 1-4, the invention is an embryo culture dish with automatic imaging and automatic liquid changing functions, comprising a box body 10, wherein a first rotating cavity 16 is arranged in the box body 10, an illumination assembly 96 is arranged in the first rotating cavity 16, a culture dish 9 is arranged on the lower end wall of the first rotating cavity 16, automatic liquid changing assemblies 97 are arranged on two sides of the culture dish 9, a second rotating cavity 47 is arranged below the culture dish 9, an automatic imaging assembly 60 is arranged in the second rotating cavity 47, a third rectangular groove 54 is arranged below the second rotating cavity 47, a fourth rectangular groove 64 is arranged on the left side of the third rectangular groove 54, a fifth rotating groove 11 is arranged on the upper end wall of the fourth rectangular groove 64, a second rectangular groove 46 is arranged on the right side of the third rectangular groove 54, a fourth rotating groove 49 penetrating through the third rectangular groove 54 is arranged between the fourth rectangular groove 64 and the second rectangular groove 46, a ninth rotating shaft 48 is arranged in the fourth rotating groove 49, the left end of the ninth rotating shaft 48 extends into the fourth rectangular groove 64 and is fixedly provided with an eleventh bevel gear 63, the right end of the ninth rotating shaft 48 extends into the second rectangular groove 46 and is fixedly provided with a seventh bevel gear 45, the ninth rotating shaft 48 is also fixedly provided with a ninth bevel gear 51 which is in power connection with the automatic imaging component 60 in the third rectangular groove 54, the left side of the first rotating cavity 16 is provided with a heating cavity 15, the heating cavity 15 is internally provided with a heating component 95, the right side of the heating cavity 15 is provided with a second transmission groove 74, the second transmission groove 74 is internally provided with a driving mechanism 100 which drives the heating component 95, the right side of the second transmission groove 74 is provided with a first rectangular groove 24, the first rectangular groove 24 is internally provided with a first transmission mechanism 98, the right side of the first rectangular groove 24 is provided with a first transmission groove 28, a second transmission mechanism 99 is disposed in the first transmission groove 28, a first rotary groove 18 penetrating the first rectangular groove 24 is disposed between the second transmission groove 74 and the first transmission groove 28, and a third rotary groove 43 communicating with each other is disposed between the first transmission groove 28 and the second rectangular groove 46.
Advantageously, said lighting assembly 96 comprises a fourth shaft 23 fixedly mounted on the bottom wall of said housing 10, the lower end of the fourth rotating shaft 23 extends into the first rotating cavity 16 and is fixedly provided with a fourth belt pulley 3, a fixed plate 86 is fixedly arranged on the fourth rotating shaft 23, a sliding plate 87 is fixedly arranged on the lower end wall of the fixed plate 86, a slide block 89 is arranged in the slide groove of the slide plate 87 and can slide left and right, a second fixed rod 88 is fixedly arranged in the slide block 89, the lower end of the sliding block 89 is fixedly provided with an installation plate 90, the lower end of the installation plate 90 is fixedly provided with an incandescent lamp 91, the sliding plate 87 is also fixedly provided with a third belt wheel 85, the third belt wheel 85 is in power connection with the fourth belt wheel 3 through a second belt 84, a third fixing rod 94 is fixedly mounted on the third pulley 85, and the third fixing rod 94 is fixedly connected with the second fixing rod 88 through a second connecting rod 93.
Beneficially, the automatic liquid changing assembly 97 includes a new culture liquid pool 14 and a waste liquid pool 36 located at two sides of the culture dish 9, a liquid inlet pipe 13 is fixedly installed between a right end wall of the new culture liquid pool 14 and a left end wall of the culture dish 9, a first water pump 12 is fixedly installed in the liquid inlet pipe 13, a twelfth rotating shaft 66 located in the fifth rotating groove 11 is dynamically installed at a lower end of the first water pump 12, a lower end of the twelfth rotating shaft 66 extends into the fourth rectangular groove 64 and is fixedly installed with a twelfth bevel gear 65 engaged with the eleventh bevel gear 63, a liquid outlet pipe 35 is fixedly installed between a right end wall of the culture dish 9 and a left end wall of the waste liquid pool 36, a second water pump 37 is fixedly installed in the liquid outlet pipe 35, an eighth rotating shaft 42 located in the third rotating groove 43 is dynamically installed at a lower end of the second water pump 37, and a lower end of the eighth rotating shaft 42 extends into the second rectangular groove 46 and is fixedly installed with a sixth bevel gear engaged with the seventh bevel gear 45 A bevel gear 44, a seventh rotating shaft 34 positioned in the third rotating groove 43 is dynamically installed at the upper end of the second water pump 37, the upper end of the seventh rotating shaft 34 extends into the first transmission groove 28 and is fixedly installed with a fifth bevel gear 33, and a culture hole 41 for placing the embryo 40 is further arranged in the culture dish 9.
Advantageously, the automatic imaging assembly 60 comprises a toothed sleeve 53 engaged with the ninth bevel gear 51, a supporting seat 59 is fixedly mounted on an outer end wall of the toothed sleeve 53, a tenth rotating shaft 56 is rotatably mounted on the supporting seat 59 through a bearing, a right end of the tenth rotating shaft 56 extends out of the supporting seat 59 and is fixedly mounted with a tenth bevel gear 55, a roller 58 is fixedly mounted on the tenth rotating shaft 56, a track groove 57 is formed on an outer surface of the roller 58, a first fixing rod 5 is movably mounted in the track groove 57, a first connecting rod 6 is fixedly mounted on an outer end wall of the first fixing rod 5, a camera 7 is fixedly mounted on the first connecting rod 6, a second fixing shaft 8 rotatably connected with the first connecting rod 6 is further fixedly mounted on the supporting seat 59, the first connecting rod 6 is fixedly connected with the first fixing rod 5, a fixing shaft 52 is rotatably mounted in the toothed sleeve 53, an eighth bevel gear 50 engaged with the tenth bevel gear 55 is fixedly mounted at the upper end of the fixed shaft 52, and a fixed block 2 fixed on the lower end wall of the third rectangular groove 54 and rotationally matched with the ninth rotating shaft 48 is fixedly mounted at the lower end of the fixed shaft 52.
Advantageously, said heating assembly 95 comprises a thirteenth shaft 73 in power connection with said driving mechanism 100, the left end of the thirteenth rotating shaft 73 extends into the heating cavity 15 and is fixedly provided with a second gear 75, a heating box 77 is arranged in the heating cavity 15, a fourteenth rotating shaft 82 is rotatably arranged in the heating box 77, a gear plate 76 engaged with the second gear 75 is fixedly mounted at the right end of the fourteenth rotating shaft 82, a rotating frame 80 is fixedly installed on the fourteenth rotating shaft 82, a stirring rod 81 is fixedly installed on the rotating frame 80, a heating sheet 83 is fixedly arranged on the inner wall of the heating box 77, an air inlet pipe 67 is fixedly arranged on the upper end wall of the heating box 77, the upper end of the air inlet pipe 67 is provided with an air storage cavity 68 with a leftward opening, the lower end wall of the heating box 77 is fixedly provided with an air outlet pipe 78, and the tail end of the air outlet pipe 78 extends into the left end wall of the culture dish 9.
Advantageously, the driving mechanism 100 includes a second rotating shaft 17 located in the first rotating groove 18, a left end of the second rotating shaft 17 extends into the air storage chamber 68 and is fixedly mounted with a fan 69, a right end of the second rotating shaft 17 extends into the first rectangular groove 24 and is fixedly mounted with a first bevel gear 20, a first pulley 70 is also fixedly mounted on the second rotating shaft 17 located in the second rotating groove 74, a right end of the thirteenth rotating shaft 73 extends into the second rotating groove 74 and is fixedly mounted with a second pulley 72, and the first pulley 70 and the second pulley 72 are in power connection through a first belt 71.
Advantageously, the first transmission mechanism 98 comprises a motor 22 in power connection with the upper end of the fourth rotating shaft 23, a second bevel gear 21 engaged with the first bevel gear 20 is fixedly mounted on the fourth rotating shaft 23 in the first rectangular groove 24, a fifth rotating shaft 26 is rotatably mounted in the first rotating groove 18 through a bearing, and the left end of the fifth rotating shaft 26 extends into the first rectangular groove 24 and is fixedly mounted with a third bevel gear 25 engaged with the second bevel gear 21.
Advantageously, the second transmission mechanism 99 comprises a fourth bevel gear 32 engaged with the fifth bevel gear 33, a sixth rotating shaft 31 is fixedly mounted at the right end of the fourth bevel gear 32, a first gear 30 is fixedly mounted at the right end of the sixth rotating shaft 31, and a incomplete gear 29 engaged with the first gear 30 is fixedly mounted at the right end of the fifth rotating shaft 26 and extends into the first transmission groove 28.
In the initial state of the apparatus of the present invention, the incandescent lamp 91 is in the non-operating state, the first water pump 12 and the second water pump 37 are both in the off state, the culture dish 9 is not filled with culture solution, the camera 7 is in the initial position in the track groove 57, the fan 69 is in the non-operating state, and the heating unit 95 is in the non-operating state.
In use, an embryo to be cultured is placed in the culture hole 41, and then the motor 22 is driven in a forward direction, the motor 22 drives the fourth rotating shaft 23 to rotate, the fourth rotating shaft 23 can drive the first bevel gear 20 and the third bevel gear 25 to rotate through the second bevel gear 21, the first bevel gear 20 can drive the first pulley 70 and the fan 69 to rotate through the second rotating shaft 17, the fan 69 can suck air into the air storage cavity 68, the first pulley 70 can drive the second pulley 72 to rotate through the first belt 71, the second pulley 72 can drive the thirteenth rotating shaft 73 to rotate, the thirteenth rotating shaft 73 can drive the toothed disc 76 to rotate through the second gear 75, the toothed disc 76 can drive the rotating frame 80 to rotate through the fourteenth rotating shaft 82, the rotating frame 80 can drive the stirring rod 81 to stir the air entering from the air inlet pipe 67, the heating box 77 can heat air through the heating plate 83, the heated air enters the culture dish 9 through the air outlet pipe 78, so as to adjust the temperature in the culture dish 9, the fourth rotating shaft 23 can drive the illumination component 96 to rotate through the fixing plate 86 on the one hand, the fourth rotating shaft 23 can also drive the fourth pulley 3 to rotate, the fourth pulley 3 can drive the third pulley 85 to rotate through the second belt 84, the third pulley 85 can drive the second connecting rod 93 to rotate through the third fixing rod 94, the second connecting rod 93 can drive the sliding block 89 to slide left and right through the second fixing rod 88, the sliding block 89 can drive the incandescent lamp 91 to slide left and right through the mounting plate 90, so as to illuminate the embryo 40, the third bevel gear 25 can drive the incomplete gear 29 to rotate through the fifth rotating shaft 26, the incomplete gear 29 can drive the sixth rotating shaft 31 to rotate through the first gear 30, the sixth rotating shaft 31 can drive the fifth bevel gear 33 to rotate through the fourth bevel gear 32, the fifth bevel gear 33 can drive the second water pump 37 to operate through the seventh rotating shaft 34, the second water pump 37 can convey the waste liquid in the waste liquid tank 36 which exceeds the liquid level 38 in the waste liquid tank 9 through the liquid outlet pipe 35, the second water pump 37 can also drive the sixth bevel gear 44 to rotate through the eighth rotating shaft 42, the sixth bevel gear 44 can drive the ninth rotating shaft 48 to rotate through the seventh bevel gear 45, the ninth rotating shaft 48 can drive the twelfth bevel gear 65 to rotate through the eleventh bevel gear 63, and the twelfth bevel gear 65 can drive the first water pump 12 to operate through the twelfth rotating shaft 66, the first water pump 12 can deliver the new culture solution in the new culture solution pool 14 to the culture dish 9, the ninth rotating shaft 48 can also drive the supporting seat 59 to rotate through the toothed sleeve 53, the supporting seat 59 can drive the first fixing rod 5 to rotate through the first connecting rod 6, the first fixing rod 5 can drive the camera 7 to rotate through the first connecting rod 6, the supporting seat 59 can also drive the tenth bevel gear 55 to rotate through the tenth rotating shaft 56, the tenth bevel gear 55 can drive the roller 58 to rotate through the tenth rotating shaft 56, the roller 58 can drive the first fixing rod 5 to slide left and right in the track groove 57 through the first connecting rod 6, the first fixing rod 5 can drive the camera 7 to slide left and right through the first connecting rod 6, and therefore the camera 7 can automatically take images of embryos 40 at certain time intervals, forming dynamic images of the whole process of embryo development.
The invention has the beneficial effects that: when the device is used, the motor can drive the illumination assembly to rotate, the fourth rotating shaft can drive the incandescent lamp to slide left and right by driving the sliding block to slide left and right, and the illumination of the embryos in the culture dish is homogenized due to the continuous rotation and left and right sliding motion of the incandescent lamp, so that each embryo is ensured to have enough illumination in each direction;
when in use, the second rotating shaft can suck air into the air storage cavity through the fan,
the second rotating shaft can also drive the stirring rod to rotate through belt transmission to stir the air entering from the air inlet pipe, so that the air forms vortex airflow, the time of the air in the heating box can be prolonged, and the heating uniformity of the air is improved;
during the use, the motor accessible drive the second water pump can with in surpass the waste liquid of liquid level passes through the drain pipe is carried in the waste liquid pond, simultaneously the power transmission of second water pump is to first water pump, first water pump can with new culture solution in the new culture solution pond is carried in the culture dish, because new culture solution pond can last toward carry new culture solution in the culture dish, can guarantee the nutrient of embryo is sufficient to improve embryo culture's success rate.
The foregoing shows and describes the general principles, essential features, and advantages of the invention. It will be understood by those skilled in the art that the present invention is not limited to the embodiments described above, which are merely illustrative of the principles of the invention, but that various changes and modifications may be made without departing from the spirit and scope of the invention, which fall within the scope of the invention as claimed. The scope of the invention is defined by the appended claims and equivalents thereof.

Claims (8)

1. An embryo culture dish with automatic imaging and automatic liquid changing functions comprises a box body, wherein a first rotating cavity is arranged in the box body, an illumination assembly is arranged in the first rotating cavity, a culture dish is arranged on the lower end wall of the first rotating cavity, automatic liquid changing assemblies are arranged on two sides of the culture dish, a second rotating cavity is arranged below the culture dish, an automatic imaging assembly is arranged in the second rotating cavity, a third rectangular groove is arranged below the second rotating cavity, a fourth rectangular groove is arranged on the left side of the third rectangular groove, a fifth rotating groove is arranged on the upper end wall of the fourth rectangular groove, a second rectangular groove is arranged on the right side of the third rectangular groove, a fourth rotating groove penetrating through the third rectangular groove is arranged between the fourth rectangular groove and the second rectangular groove, a ninth rotating shaft is arranged in the fourth rotating groove, the left end of the ninth rotating shaft extends into the fourth rectangular groove and is fixedly provided with an eleventh bevel gear, the right end of the ninth rotating shaft extends into the second rectangular groove and is fixedly provided with a seventh bevel gear, a ninth bevel gear which is in power connection with the automatic imaging component is also fixedly arranged on the ninth rotating shaft in the third rectangular groove, a heating cavity is arranged at the left side of the first rotating cavity, a heating assembly is arranged in the heating cavity, a second transmission groove is arranged at the right side of the heating cavity, a driving mechanism for driving the heating assembly is arranged in the second transmission groove, a first rectangular groove is arranged on the right side of the second transmission groove, a first transmission mechanism is arranged in the first rectangular groove, a first transmission groove is arranged on the right side of the first rectangular groove, and a second transmission mechanism is arranged in the first transmission groove, a first rotary groove penetrating through the first rectangular groove is arranged between the second transmission groove and the first transmission groove, and a third rotary groove communicated with each other is arranged between the first transmission groove and the second rectangular groove.
2. The embryo culture dish with automatic imaging and automatic liquid changing functions of claim 1, wherein: the illumination subassembly includes fixed mounting and is in the fourth pivot of well diapire, fourth pivot lower extreme stretches into in the first rotation chamber and fixed mounting have a fourth band pulley, fixed mounting has the fixed plate in the fourth pivot, fixed mounting has the slide under the fixed plate, but spout horizontal slip's in the slide slider install the slider, fixed mounting has the second dead lever in the slider, slider lower extreme fixed mounting has the mounting panel, mounting panel lower extreme fixed mounting has the incandescent lamp, it has the third band pulley still to fix on the slide, the third band pulley passes through second belt power connection with the fourth band pulley, fixed mounting has the third dead lever on the third band pulley, the third dead lever with the second dead lever passes through second connecting rod fixed connection.
3. The embryo culture dish with automatic imaging and automatic liquid changing functions of claim 2, wherein: the automatic liquid changing assembly comprises a new culture liquid pool and a waste liquid pool which are positioned at two sides of a culture dish, a liquid inlet pipe is fixedly arranged between the right end wall of the new culture liquid pool and the left end wall of the culture dish, a first water pump is fixedly arranged in the liquid inlet pipe, a twelfth rotating shaft positioned in a fifth rotating groove is dynamically arranged at the lower end of the first water pump, the lower end of the twelfth rotating shaft extends into the fourth rectangular groove and is fixedly provided with a twelfth bevel gear meshed with the eleventh bevel gear, a liquid outlet pipe is fixedly arranged between the right end wall of the culture dish and the left end wall of the waste liquid pool, a second water pump is fixedly arranged in the liquid outlet pipe, an eighth rotating shaft positioned in the third rotating groove is dynamically arranged at the lower end of the second water pump, the lower end of the eighth rotating shaft extends into the second rectangular groove and is fixedly provided with a sixth bevel gear meshed with the seventh bevel gear, a seventh rotating shaft positioned in the third rotating groove is dynamically installed at the upper end of the second water pump, a fifth bevel gear is fixedly installed at the upper end of the seventh rotating shaft and extends into the first transmission groove, and a culture hole for placing embryos is further formed in the culture dish.
4. The embryo culture dish with automatic imaging and automatic liquid changing functions of claim 3, wherein: the automatic imaging assembly comprises a tooth sleeve meshed with the ninth bevel gear, a supporting seat is fixedly arranged on the outer end wall of the tooth sleeve, a tenth rotating shaft is rotatably arranged on the supporting seat through a bearing, the right end of the tenth rotating shaft extends out of the supporting seat and is fixedly provided with a tenth bevel gear, a roller is fixedly arranged on the tenth rotating shaft, a track groove is arranged on the outer surface of the roller, a first fixing rod is movably arranged in the track groove, a first connecting rod is fixedly arranged on the outer end wall of the first fixing rod, a camera is fixedly arranged on the first connecting rod, a second fixing shaft rotatably connected with the first connecting rod is further fixedly arranged on the supporting seat, the first connecting rod is fixedly connected with the first fixing rod, a fixing shaft is rotatably arranged in the tooth sleeve, and an eighth bevel gear meshed with the tenth bevel gear is fixedly arranged at the upper end of the fixing shaft, and the lower end of the fixed shaft is fixedly provided with a fixed block which is fixed on the lower end wall of the third rectangular groove and is in running fit with the ninth rotating shaft.
5. An embryo culture dish with automatic imaging and automatic liquid changing functions according to claim 4, wherein: heating element include with the thirteenth pivot that actuating mechanism power is connected, thirteenth pivot left end stretches into in the heating chamber and fixed mounting have the second gear, be equipped with the heating cabinet in the heating chamber, rotationally install the fourteenth pivot in the heating cabinet, fourteenth pivot right-hand member fixed mounting have with the fluted disc of second gear engagement, fixed mounting has the swivel mount in the fourteenth pivot, fixed mounting has the stirring rod on the swivel mount, inner wall fixed mounting has the heating plate in the heating cabinet, heating cabinet upper end wall fixed mounting has the intake pipe, the intake pipe upper end is equipped with the gas storage chamber of opening left, end wall fixed mounting has the outlet duct under the heating cabinet, the outlet duct end stretches into the left end wall of culture dish.
6. An embryo culture dish with automatic imaging and automatic liquid changing functions according to claim 5, wherein: the driving mechanism comprises a second rotating shaft located in the first rotating groove, the left end of the second rotating shaft extends into the air storage cavity and is fixedly provided with a fan, the right end of the second rotating shaft extends into the first rectangular groove and is fixedly provided with a first bevel gear, the second rotating shaft is located in the second transmission groove and is further fixedly provided with a first belt wheel, the right end of the thirteenth rotating shaft extends into the second transmission groove and is fixedly provided with a second belt wheel, and the first belt wheel is in power connection with the second belt wheel through a first belt.
7. The embryo culture dish with automatic imaging and automatic liquid changing functions of claim 6, wherein: the first transmission mechanism comprises a motor in power connection with the upper end of a fourth rotating shaft, a second bevel gear meshed with the first bevel gear is fixedly installed in the first rectangular groove on the fourth rotating shaft, a fifth rotating shaft is rotatably installed in the first rotating groove through a bearing, and the left end of the fifth rotating shaft extends into the first rectangular groove and is fixedly provided with a third bevel gear meshed with the second bevel gear.
8. The embryo culture dish with automatic imaging and automatic liquid changing functions of claim 7, wherein: the second transmission mechanism comprises a fourth bevel gear meshed with the fifth bevel gear, a sixth rotating shaft is fixedly mounted at the right end of the fourth bevel gear, a first gear is fixedly mounted at the right end of the sixth rotating shaft, and an incomplete gear meshed with the first gear is fixedly mounted at the right end of the fifth rotating shaft and extends into the first transmission groove.
CN201911177086.4A 2019-11-26 2019-11-26 Embryo culture dish with automatic imaging and automatic liquid changing functions Withdrawn CN110713931A (en)

Priority Applications (1)

Application Number Priority Date Filing Date Title
CN201911177086.4A CN110713931A (en) 2019-11-26 2019-11-26 Embryo culture dish with automatic imaging and automatic liquid changing functions

Applications Claiming Priority (1)

Application Number Priority Date Filing Date Title
CN201911177086.4A CN110713931A (en) 2019-11-26 2019-11-26 Embryo culture dish with automatic imaging and automatic liquid changing functions

Publications (1)

Publication Number Publication Date
CN110713931A true CN110713931A (en) 2020-01-21

Family

ID=69216490

Family Applications (1)

Application Number Title Priority Date Filing Date
CN201911177086.4A Withdrawn CN110713931A (en) 2019-11-26 2019-11-26 Embryo culture dish with automatic imaging and automatic liquid changing functions

Country Status (1)

Country Link
CN (1) CN110713931A (en)

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