CN110710496B - Method for cleaning and purifying live golden-edged leech saliva - Google Patents
Method for cleaning and purifying live golden-edged leech saliva Download PDFInfo
- Publication number
- CN110710496B CN110710496B CN201911054477.7A CN201911054477A CN110710496B CN 110710496 B CN110710496 B CN 110710496B CN 201911054477 A CN201911054477 A CN 201911054477A CN 110710496 B CN110710496 B CN 110710496B
- Authority
- CN
- China
- Prior art keywords
- golden
- water
- cultivation
- edged
- cleaning
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Active
Links
- 241000545744 Hirudinea Species 0.000 title claims abstract description 127
- 238000004140 cleaning Methods 0.000 title claims abstract description 53
- 238000000034 method Methods 0.000 title claims abstract description 46
- 210000003296 saliva Anatomy 0.000 title claims abstract description 42
- XLYOFNOQVPJJNP-UHFFFAOYSA-N water Substances O XLYOFNOQVPJJNP-UHFFFAOYSA-N 0.000 claims abstract description 172
- 239000008280 blood Substances 0.000 claims abstract description 57
- 210000004369 blood Anatomy 0.000 claims abstract description 57
- 241001465754 Metazoa Species 0.000 claims abstract description 31
- GHXZTYHSJHQHIJ-UHFFFAOYSA-N Chlorhexidine Chemical compound C=1C=C(Cl)C=CC=1NC(N)=NC(N)=NCCCCCCN=C(N)N=C(N)NC1=CC=C(Cl)C=C1 GHXZTYHSJHQHIJ-UHFFFAOYSA-N 0.000 claims abstract description 15
- CEAZRRDELHUEMR-URQXQFDESA-N Gentamicin Chemical compound O1[C@H](C(C)NC)CC[C@@H](N)[C@H]1O[C@H]1[C@H](O)[C@@H](O[C@@H]2[C@@H]([C@@H](NC)[C@@](C)(O)CO2)O)[C@H](N)C[C@@H]1N CEAZRRDELHUEMR-URQXQFDESA-N 0.000 claims abstract description 15
- 229930182566 Gentamicin Natural products 0.000 claims abstract description 15
- 229960003260 chlorhexidine Drugs 0.000 claims abstract description 15
- 229960002518 gentamicin Drugs 0.000 claims abstract description 15
- 238000005406 washing Methods 0.000 claims abstract description 14
- 238000002791 soaking Methods 0.000 claims abstract description 7
- 239000010865 sewage Substances 0.000 claims description 22
- 241000607528 Aeromonas hydrophila Species 0.000 claims description 11
- 210000001503 joint Anatomy 0.000 claims description 11
- 239000000463 material Substances 0.000 claims description 11
- 238000003307 slaughter Methods 0.000 claims description 10
- 241000272525 Anas platyrhynchos Species 0.000 claims description 9
- RYGMFSIKBFXOCR-UHFFFAOYSA-N Copper Chemical compound [Cu] RYGMFSIKBFXOCR-UHFFFAOYSA-N 0.000 claims description 8
- 229910052785 arsenic Inorganic materials 0.000 claims description 8
- RQNWIZPPADIBDY-UHFFFAOYSA-N arsenic atom Chemical compound [As] RQNWIZPPADIBDY-UHFFFAOYSA-N 0.000 claims description 8
- 229910052793 cadmium Inorganic materials 0.000 claims description 8
- BDOSMKKIYDKNTQ-UHFFFAOYSA-N cadmium atom Chemical compound [Cd] BDOSMKKIYDKNTQ-UHFFFAOYSA-N 0.000 claims description 8
- 229910052802 copper Inorganic materials 0.000 claims description 8
- 239000010949 copper Substances 0.000 claims description 8
- QSHDDOUJBYECFT-UHFFFAOYSA-N mercury Chemical compound [Hg] QSHDDOUJBYECFT-UHFFFAOYSA-N 0.000 claims description 8
- 229910052753 mercury Inorganic materials 0.000 claims description 8
- 241000607534 Aeromonas Species 0.000 claims description 7
- 239000012780 transparent material Substances 0.000 claims description 6
- 238000007789 sealing Methods 0.000 claims description 5
- 241000589516 Pseudomonas Species 0.000 claims description 4
- 241000287828 Gallus gallus Species 0.000 claims description 3
- 241000283690 Bos taurus Species 0.000 claims description 2
- 241001494479 Pecora Species 0.000 claims description 2
- 241000282898 Sus scrofa Species 0.000 claims description 2
- 230000006866 deterioration Effects 0.000 claims description 2
- 239000011521 glass Substances 0.000 abstract description 12
- 241000607598 Vibrio Species 0.000 abstract 2
- 244000000007 bacterial human pathogen Species 0.000 abstract 1
- 239000000243 solution Substances 0.000 description 24
- 241000604933 Bdellovibrio Species 0.000 description 5
- 244000052616 bacterial pathogen Species 0.000 description 5
- 238000001514 detection method Methods 0.000 description 5
- 238000009395 breeding Methods 0.000 description 4
- 230000001488 breeding effect Effects 0.000 description 4
- 241000894006 Bacteria Species 0.000 description 3
- 201000010099 disease Diseases 0.000 description 3
- 208000037265 diseases, disorders, signs and symptoms Diseases 0.000 description 3
- 230000001717 pathogenic effect Effects 0.000 description 3
- 239000007864 aqueous solution Substances 0.000 description 2
- 238000012364 cultivation method Methods 0.000 description 2
- 244000052769 pathogen Species 0.000 description 2
- 238000009360 aquaculture Methods 0.000 description 1
- 244000144974 aquaculture Species 0.000 description 1
- 230000009286 beneficial effect Effects 0.000 description 1
- 239000012153 distilled water Substances 0.000 description 1
- 239000003814 drug Substances 0.000 description 1
- 230000000694 effects Effects 0.000 description 1
- 238000001914 filtration Methods 0.000 description 1
- 241000411851 herbal medicine Species 0.000 description 1
- 244000052637 human pathogen Species 0.000 description 1
- 238000012986 modification Methods 0.000 description 1
- 230000004048 modification Effects 0.000 description 1
Classifications
-
- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01K—ANIMAL HUSBANDRY; AVICULTURE; APICULTURE; PISCICULTURE; FISHING; REARING OR BREEDING ANIMALS, NOT OTHERWISE PROVIDED FOR; NEW BREEDS OF ANIMALS
- A01K67/00—Rearing or breeding animals, not otherwise provided for; New or modified breeds of animals
- A01K67/033—Rearing or breeding invertebrates; New breeds of invertebrates
Landscapes
- Life Sciences & Earth Sciences (AREA)
- Environmental Sciences (AREA)
- Animal Behavior & Ethology (AREA)
- Zoology (AREA)
- Animal Husbandry (AREA)
- Biodiversity & Conservation Biology (AREA)
- Farming Of Fish And Shellfish (AREA)
- Catching Or Destruction (AREA)
Abstract
The invention provides a method for cleaning and purifying live golden-edged leech saliva, which comprises the following steps: 1) Adding cultivation water into a large-mouth glass bottle, and placing the golden-edged leech larvae into a cultivation device for cultivation; 2) Collecting fresh and clean animal blood, filling an artificial casing, and then feeding the leeches with golden edges in a feeding device for continuous cultivation; 3) In the cultivation process, the golden-edged leeches are placed in gentamicin solution with 8-12 ten thousand units per 100mL for 2-4 hours every two weeks, and then water is changed for cleaning; 4) Stopping feeding 2-3 months before sale, and soaking and washing the leech with 0.02-0.1% chlorhexidine water solution for 10-20min before sale to finish cleaning and purifying the leech saliva. The golden-edged leech saliva cultivated by the invention has the characteristics of reducing the content of vibrio bacteria of the vibrio genus of the human pathogenic bacteria, saving water in the cultivation process and the like.
Description
Technical Field
The invention belongs to the field of aquaculture. More particularly, the invention relates to a method for cleaning and purifying live golden-edged leech saliva.
Background
Leech with golden edge is listed in a second book of 'Guangxi Zhuang nationality Zhuang medicinal materials standard'. The Chinese herbal medicine is widely used as a medicine for treating diseases in Guangxi Zhuang nationality, and can also be used for treating diseases by sucking blood. However, the growth environment of the golden-edged leeches is complex, and various human pathogens can be carried in the body, so that when the golden-edged leeches are used for sucking blood and treating diseases, the pathogens carried by the golden-edged leeches are also injected into the human body when the saliva of the golden-edged leeches is injected into the human body, and the human body is infected. Therefore, the establishment of the method for cleaning and purifying the living golden-edged leech saliva has important significance. The method has the effect of obviously reducing the pathogenic bacteria of the single package bacteria in the live golden-edged leech saliva.
Disclosure of Invention
It is an object of the present invention to address at least the above problems and/or disadvantages and to provide at least the advantages described below.
The invention also aims to provide a method for cleaning and purifying the live golden-edged leech saliva, which can save the water for cultivation and is convenient for feeding and raising.
To achieve these objects and other advantages and in accordance with the purpose of the invention, there is provided a method for cleaning and purifying live golden-edged leech saliva, comprising the steps of:
1) Adding cultivation water into a large-mouth glass bottle, and placing the golden-edged leech larvae into a cultivation device for cultivation;
2) Collecting fresh and clean animal blood, filling an artificial casing, and then feeding the leeches with golden edges in a feeding device for continuous cultivation;
3) In the cultivation process, the golden-edged leeches are placed in gentamicin solution with 8-12 ten thousand units per 100mL for 2-4 hours every two weeks, and then water is changed for cleaning;
4) Stopping feeding 2-3 months before sale, and soaking and washing the leech with 0.02-0.1% chlorhexidine water solution for 10-20min before sale to finish cleaning and purifying the leech saliva.
Preferably, the water for cultivation meets the requirements of the regulations of the fishery water quality standard (GB 11607), and the pH value of the water body is 6.5-7.0.
Preferably, the animal blood is chicken, duck, pig, cow, sheep blood.
Preferably, the animal blood is required to be free from pollution, deterioration and addition of any auxiliary materials, and the golden-edged leech feeding should be completed within 4 hours from the time of slaughtering the blood.
Preferably, lead in the animal blood is less than or equal to 10mg/kg; cadmium is less than or equal to 0.8mg/kg; arsenic is less than or equal to 2mg/kg; mercury is less than or equal to 0.2mg/kg; copper is less than or equal to 15mg/kg.
Preferably, the blood of the animal does not contain one or more of leech pseudomonas, aeromonas hydrophila or aeromonas spot.
Preferably, the cultivation device comprises:
breed the case, its cross section is the box of rectangle, breed the case and make by transparent material, the top of breeding the case is provided with the rectangle feed inlet along its width direction, breed the bottom of case be provided with the drain that the feed inlet corresponds, the left and right sides of the drain of breeding the case bottom all is provided with first protruding edge, the left and right sides of drain all is provided with the breakwater, and the breakwater passes through the hinge and articulates when the bottom of breeding the case is so that the breakwater rotates to the bottom with breeding the case perpendicularly, the lower extreme and the first protruding edge butt of breakwater, the other end that the breakwater kept away from articulated department is provided with the engaging lug, the below of feed inlet is provided with the locating plate, is provided with the electric telescopic handle with the connecting lug on the left side breakwater between the breakwater, and the other end and the connecting lug on the right side breakwater of electric telescopic handle articulate, be provided with the second protruding edge on the inside wall of both sides around the case of breed the case, the width is less than the bottom of the electric telescopic handle and the base of a rectangle side wall when the expansion joint of the bottom of the expansion handle and the bottom of the expansion handle make the bottom of the expansion handle. The water body in the feeding area can be discharged after the golden-edge leeches are fed through the water baffle to form a closed feeding area, so that other water bodies in the cultivation box body are prevented from being polluted, and the using amount of cultivation water is reduced.
Preferably, the drain outlet is provided with an escape preventing net, a valve switch is arranged below the escape preventing net to open or close the drain outlet, and the feed inlet is provided with an escape preventing device.
Preferably, sealing strips are arranged at the edges of the water baffles at the left side and the right side so that the edges of the water baffles at the left side and the right side are tightly connected with the first convex edge and the second convex edge when the water baffles are pulled up by the electric telescopic rod.
Preferably, the bottoms of the culture boxes at the two sides of the sewage outlet are inclined at an angle of 5-10 degrees with the horizontal ground. Through the bottom plate and the horizontal ground slope setting of the breed case with drain both sides for the breakwater also is the slope at ordinary times, makes an contained angle between left side breakwater and the right side breakwater, when electric telescopic handle is in the shrink, the left side breakwater and the right side breakwater of will more easily pull up.
The invention at least comprises the following beneficial effects: the growth of pathogenic bacteria of the monad genus in the land leeches can be effectively inhibited by strictly controlling the feeding conditions of the land leeches; in the cultivation process, gentamicin aqueous solution is used for soaking treatment, and before the leech with golden edge is marketed, the pathogenic bacteria of the monad bacteria in the leech with golden edge can be effectively inhibited by stopping feeding and using 0.02% chlorhexidine aqueous solution for soaking and washing for 10-15 minutes and then cleaning with clean water; when the golden leech needs to be fed, the left water baffle and the right water baffle are retracted through the electric telescopic rods, so that the tops of the left water baffle and the right water baffle are abutted on the positioning plate, the edges of the left water baffle and the right water baffle are abutted on the second convex edge, the bottoms of the left water baffle and the right water baffle are abutted on the first convex edge, a feeding area with a rectangular cross section is formed in the culture box through the two water baffles, water between the water baffles is discharged from the tops of the water baffles or the water filtering holes and enters the culture box outside the feeding area, at the moment, leeches are concentrated in the feeding area, the feeding device filled with blood is placed in the feeding area for feeding leeches, after feeding is completed, the feeding device is taken out, a valve on a sewage draining outlet is opened for draining sewage in the feeding area, the golden leech is flushed by using a clean water body, the valve on the sewage draining outlet is closed, and the electric telescopic rods are stretched to push the water baffles to the outside to enable the water baffles to be abutted on the bottom of the culture box, and the golden leech is returned to the culture box again. The golden-edged leeches cultivated by the method have the characteristics of obviously reducing pathogenic bacteria of monad bacteria of human bodies, saving water in the cultivation process and the like.
Additional advantages, objects, and features of the invention will be set forth in part in the description which follows and in part will become apparent to those having ordinary skill in the art upon examination of the following or may be learned from practice of the invention.
Drawings
FIG. 1 is a schematic structural view of a cultivation device according to the present invention, wherein the water baffles on the left and right sides are between retracted and square at the bottom of the cultivation box;
FIG. 2 is a schematic structural view of a cultivation device according to another embodiment of the invention, wherein the bottom of the cultivation box forms an included angle with the horizontal ground.
1. A cultivation box; 2. a feed port; 3. a sewage outlet; 4. a left water baffle; 5. a positioning plate; 6. an electric telescopic rod; 7. and a right water baffle.
Detailed Description
The present invention is described in further detail below with reference to the drawings to enable those skilled in the art to practice the invention by referring to the description.
It will be understood that terms, such as "having," "including," and "comprising," as used herein, do not preclude the presence or addition of one or more other elements or groups thereof.
Example 1
A method for cleaning and purifying living golden-edged leech saliva comprises the following steps:
1) Adding cultivation water into a large-mouth glass bottle, and placing the golden-edged leech larvae into a cultivation device for cultivation;
2) Collecting fresh and clean animal blood, filling an artificial casing, and then feeding the leeches with golden edges in a feeding device for continuous cultivation;
3) In the cultivation process, the golden-edged leeches are placed in gentamicin solution with 8 ten thousand units per 100mL for 2 hours every two weeks, and then water is changed for cleaning;
4) Stopping feeding 2 months before sale, and immersing and washing the golden-edged leeches in 0.02% chlorhexidine water solution for 10 minutes before sale, thus completing the cleaning and purifying of golden-edged leech saliva.
Example 2
A method for cleaning and purifying living golden-edged leech saliva comprises the following steps:
1) Adding cultivation water into a large-mouth glass bottle, and placing the golden-edged leech larvae into a cultivation device for cultivation;
2) Collecting fresh and clean chicken blood, filling an artificial casing, and then feeding the leeches with golden edges in a feeding device for continuous cultivation;
3) In the cultivation process, the golden-edged leeches are placed in gentamicin solution with the concentration of 12 ten thousand units per 100mL every two weeks for soaking and cultivating for 4 hours, and then water is changed for cleaning;
4) Stopping feeding 3 months before sale, and immersing and washing the golden-edged leeches in 0.05% chlorhexidine water solution for 20 minutes before sale, thus completing the cleaning and purifying of golden-edged leech saliva.
Example 3
A method for cleaning and purifying living golden-edged leech saliva comprises the following steps:
1) Adding cultivation water into a large-mouth glass bottle, and placing the golden-edged leech larvae into a cultivation device for cultivation;
2) Collecting fresh and clean pig blood, filling an artificial casing, and then filling the pig blood into a feeding device to feed the golden-edged leeches, wherein the animal blood is not polluted, deteriorated and auxiliary materials are not added, and the golden-edged leeches are fed within 4 hours from the time of slaughtering the blood, so that the pig blood is continuously cultivated;
3) In the cultivation process, the golden-edged leeches are placed in 10 ten thousand units per 100mL gentamicin solution every two weeks for soaking and cultivating for 4 hours, and then water is changed for cleaning;
4) Stopping feeding 3 months before sale, and immersing and washing the golden-edged leeches in 0.1% chlorhexidine water solution for 20 minutes before sale, thus completing the cleaning and purifying of golden-edged leech saliva. .
Example 4
A method for cleaning and purifying living golden-edged leech saliva comprises the following steps:
1) Adding cultivation water into a large-mouth glass bottle, and placing the golden-edged leech larvae into a cultivation device for cultivation; wherein, the water for cultivation meets the specified requirement of fishery water quality standard (GB 11607), and the pH value of the water body is 7.0;
2) Collecting fresh and clean duck blood, filling an artificial casing, and then filling the artificial casing into a feeding device to feed the golden-edged leeches, wherein the animal blood is not polluted, deteriorated and auxiliary materials are not added, and the golden-edged leeches are fed within 4 hours from the time of slaughtering the blood, so that the continuous cultivation is realized;
3) In the cultivation process, the golden-edged leeches are placed in gentamicin solution with 8 ten thousand units per 100mL for 4 hours every two weeks, and then water is changed for cleaning;
4) Stopping feeding 3 months before sale, and immersing and washing the golden-edged leeches in 0.02% chlorhexidine water solution for 20 minutes before sale, thus completing the cleaning and purifying of golden-edged leech saliva.
Example 5
A method for cleaning and purifying living golden-edged leech saliva comprises the following steps:
1) Adding cultivation water into a large-mouth glass bottle, and placing the golden-edged leech larvae into a cultivation device for cultivation; wherein, the water for cultivation meets the specified requirement of fishery water quality standard (GB 11607), and the pH value of the water body is 6.5;
2) Collecting fresh and clean duck blood, filling an artificial casing, and then filling the artificial casing into a feeding device to feed the golden-edged leeches, wherein the animal blood is not polluted, is not deteriorated, is not added with any auxiliary materials, and the golden-edged leech feeding is completed within 4 hours from the time of slaughtering the blood, and the lead in the animal blood is less than or equal to 10mg/kg; cadmium is less than or equal to 0.8mg/kg; arsenic is less than or equal to 2mg/kg; mercury is less than or equal to 0.2mg/kg; copper is less than or equal to 15mg/kg, and continuous cultivation is carried out;
3) In the cultivation process, the golden-edged leeches are placed in 10 ten thousand units per 100mL gentamicin solution every two weeks for 3 hours, and then water is changed for cleaning;
4) Stopping feeding 2 months before sale, and immersing and washing the golden-edged leeches in 0.02% chlorhexidine water solution for 15 minutes before sale, thus completing the cleaning and purifying of golden-edged leech saliva.
Example 6
A method for cleaning and purifying living golden-edged leech saliva comprises the following steps:
1) Adding cultivation water into a large-mouth glass bottle, and placing the golden-edged leech larvae into a cultivation device for cultivation; wherein, the water for cultivation meets the specified requirement of fishery water quality standard (GB 11607), and the pH value of the water body is 6.5;
2) Collecting fresh and clean duck blood, filling an artificial casing, and then filling the artificial casing into a feeding device to feed the golden-edged leeches, wherein the animal blood is not polluted, is not deteriorated, is not added with any auxiliary materials, and the golden-edged leech feeding is completed within 4 hours from the time of slaughtering the blood, and the lead in the animal blood is less than or equal to 10mg/kg; cadmium is less than or equal to 0.8mg/kg; arsenic is less than or equal to 2mg/kg; mercury is less than or equal to 0.2mg/kg; copper is less than or equal to 15mg/kg, and the animal blood does not contain Bdellovibrio pseudomonad, aeromonas hydrophila and aeromonas maculosa;
3) In the cultivation process, the golden-edged leeches are placed in gentamicin solution with 8 ten thousand units per 100mL for 2 hours every two weeks, and then water is changed for cleaning;
4) Stopping feeding 2 months before sale, and immersing and washing the golden-edged leeches in 0.02% chlorhexidine water solution for 10 minutes before sale, thus completing the cleaning and purifying of golden-edged leech saliva.
Example 7
A method for cleaning and purifying living golden-edged leech saliva comprises the following steps:
1) Adding cultivation water into a large-mouth glass bottle, and placing the golden-edged leech larvae into a cultivation device for cultivation; wherein, the water for cultivation meets the specified requirement of fishery water quality standard (GB 11607), and the pH value of the water body is 7.0; as shown in fig. 1, the cultivation device includes:
the cross section of the cultivation box 1 is a rectangular box body, the cultivation box 1 is made of transparent materials, a rectangular feeding opening 2 is arranged at the top of the cultivation box 1 along the width direction of the cultivation box, a positioning plate 5 is arranged below the feeding opening 2, an electric telescopic rod 6 with a waterproof function is arranged between the left side and the right side of the feeding opening 2, first convex edges are arranged on the left side and the right side of the sewage opening 3 at the bottom of the cultivation box 1, water baffles are arranged on the left side and the right side of the sewage opening 3, when the water baffles on the left side and the right side are hinged to the bottom of the cultivation box through hinges so that the water baffles on the left side and the right side rotate to the position perpendicular to the bottom of the cultivation box 1, connecting lugs are arranged at the lower ends of the water baffles on the left side and the right side, the water baffles are far away from the other ends of the hinged positions, a positioning plate 5 is arranged below the feeding opening 2, one end of the electric telescopic rod 6 is hinged with the connecting lugs on the left side 4, the other end of the electric telescopic rod 6 is hinged with the connecting lugs on the right side of the left side of the water baffle 7, the electric telescopic rod 6 is abutted to the two side walls of the water baffles on the bottom of the cultivation box 1, and the two side walls of the electric telescopic rod are abutted to the two side walls of the water baffles on the bottom of the rectangular box 1, and the bottom of the electric telescopic rod is abutted to the two side of the bottom of the water baffles on the bottom of the rectangular box is abutted to the bottom of the first side of the water baffles, and the bottom of the electric expansion plate is abutted to the bottom of the rectangular box, and the bottom is abutted to the bottom of the water baffle;
2) Collecting fresh and clean duck blood, filling an artificial casing, and then filling the artificial casing into a feeding device to feed the golden-edged leeches, wherein the animal blood is not polluted, is not deteriorated, is not added with any auxiliary materials, and the golden-edged leech feeding is completed within 4 hours from the time of slaughtering the blood, and the lead in the animal blood is less than or equal to 10mg/kg; cadmium is less than or equal to 0.8mg/kg; arsenic is less than or equal to 2mg/kg; mercury is less than or equal to 0.2mg/kg; copper is less than or equal to 15mg/kg, and the animal blood does not contain Bdellovibrio pseudomonad, aeromonas hydrophila and aeromonas maculosa;
3) In the cultivation process, the golden-edged leeches are placed in gentamicin solution with 8 ten thousand units per 100mL for 2 hours every two weeks, and then water is changed for cleaning;
4) Stopping feeding 2 months before sale, and immersing and washing the golden-edged leeches in 0.02% chlorhexidine water solution for 10 minutes before sale, thus completing the cleaning and purifying of golden-edged leech saliva.
The water body in the feeding area can be discharged after the golden-edge leeches are fed through the water baffle to form a closed feeding area, so that other water bodies in the culture box 1 are prevented from being polluted, and the using amount of culture water is reduced.
Example 8
A method for cleaning and purifying living golden-edged leech saliva comprises the following steps:
1) Adding cultivation water into a large-mouth glass bottle, and placing the golden-edged leech larvae into a cultivation device for cultivation; wherein, the water for cultivation meets the specified requirement of fishery water quality standard (GB 11607), and the pH value of the water body is 6.5; as shown in fig. 1, the cultivation device includes:
the cross section of the cultivation box 1 is a rectangular box body, the cultivation box 1 is made of transparent materials, a rectangular feeding opening 2 is arranged at the top of the cultivation box 1 along the width direction of the cultivation box, a positioning plate 5 is arranged below the feeding opening 2, an electric telescopic rod 6 with a waterproof function is arranged between the left side and the right side of the feeding opening 2, first convex edges are arranged on the left side and the right side of the sewage opening 3 at the bottom of the cultivation box 1, water baffles are arranged on the left side and the right side of the sewage opening 3, the water baffles are hinged to the top of the first convex edges through hinges so that when the water baffles on the left side and the right side rotate to the position perpendicular to the bottom of the cultivation box 1, the lower ends of the water baffles on the left side and the right side are abutted to the first convex edges, the other ends of the water baffles on the left side and the right side are far away from the hinged position, a connecting lug is arranged below the feeding opening 2, one end of the electric telescopic rod 6 is hinged to the connecting lug on the left side plate 4, the other end of the electric telescopic rod 6 is hinged to the connecting lug on the right side of the electric telescopic rod 4, the electric telescopic rod 6 is abutted to the bottom of the water baffles on the first side of the electric box, and the two sides of the electric telescopic rod 6 are abutted to the bottom of the first convex edges of the water baffles are abutted to the bottom of the rectangular box 1, and the bottom of the electric feeding opening 6 is abutted to the bottom of the first convex edge, and the bottom of the electric expansion rod is abutted to the first side of the water baffles is abutted to the bottom of the rectangular box, and the bottom of the first side of the water baffle is abutted to the bottom; the sewage outlet 3 is provided with an escape preventing net, a valve switch is arranged below the escape preventing net to open or close the sewage outlet 3, and an escape preventing device is arranged at the feeding port 2;
2) Collecting fresh and clean duck blood, filling an artificial casing, and then filling the artificial casing into a feeding device to feed the golden-edged leeches, wherein the animal blood is not polluted, is not deteriorated, is not added with any auxiliary materials, and the golden-edged leech feeding is completed within 4 hours from the time of slaughtering the blood, and the lead in the animal blood is less than or equal to 10mg/kg; cadmium is less than or equal to 0.8mg/kg; arsenic is less than or equal to 2mg/kg; mercury is less than or equal to 0.2mg/kg; copper is less than or equal to 15mg/kg, and the animal blood does not contain Bdellovibrio pseudomonad, aeromonas hydrophila and aeromonas maculosa;
3) In the cultivation process, the golden-edged leeches are placed in 10 ten thousand units per 100mL gentamicin solution every two weeks for 3 hours, and then water is changed for cleaning;
4) Stopping feeding 3 months before sale, and immersing and washing the golden-edged leeches in 0.02% chlorhexidine water solution for 10 minutes before sale, thus completing the cleaning and purifying of golden-edged leech saliva.
Example 9
A method for cleaning and purifying living golden-edged leech saliva comprises the following steps:
1) Adding cultivation water into a large-mouth glass bottle, and placing the golden-edged leech larvae into a cultivation device for cultivation; wherein, the water for cultivation meets the specified requirement of fishery water quality standard (GB 11607), and the pH value of the water body is 6.5; as shown in fig. 1, the cultivation device includes:
the cross section of the cultivation box 1 is rectangular, the cultivation box 1 is made of transparent materials, a rectangular feeding opening 2 is arranged at the top of the cultivation box 1 along the width direction of the cultivation box, a drain outlet 3 corresponding to the feeding opening 2 is arranged at the bottom of the cultivation box 1, first convex edges are arranged on the left side and the right side of the drain outlet 3 at the bottom of the cultivation box 1, water baffles are arranged on the left side and the right side of the drain outlet 3, the water baffles are respectively hinged to the bottom of the cultivation box through hinges, when the water baffles on the left side and the right side rotate to the position vertical to the bottom of the cultivation box 1, the lower ends of the water baffles on the left side and the right side are abutted against the first convex edges, a connecting lug is arranged at one end of the water baffles on the left side and the right side, which is far away from the hinged position, a positioning plate 5 is arranged below the feed inlet 2, an electric telescopic rod 6 with a waterproof function is arranged between the water baffles at the left side and the right side, one end of the electric telescopic rod 6 is hinged with a connecting lug on the water baffle at the left side, the other end of the electric telescopic rod 6 is hinged with a connecting lug on the water baffle at the right side 7, second convex edges are arranged on the inner side walls of the front side and the rear side of the box body of the cultivation box 1, the basic length of the electric telescopic rod 6 is smaller than the width of the sewage outlet 3 so that the two sides of the water baffle are in butt joint with the second convex edges when the electric telescopic rod 6 contracts, the top of the water baffle is in butt joint with the positioning plate 5, the bottom of the water baffle is in butt joint with the first convex edges, and the water baffles at the left side and the right side and the side wall and the bottom of the cultivation box 1 are enclosed into a feeding area with rectangular cross section; the sewage outlet 3 is provided with an escape preventing net, a valve switch is arranged below the escape preventing net to open or close the sewage outlet 3, and an escape preventing device is arranged at the feeding port 2; the edges of the water baffles on the left side and the right side are provided with sealing strips so that when the water baffles are pulled up by the electric telescopic rod 6, the edges of the water baffles on the left side and the right side are tightly connected with the first convex edge and the second convex edge;
2) Collecting fresh and clean duck blood, filling an artificial casing, and then filling the artificial casing into a feeding device to feed the golden-edged leeches, wherein the animal blood is not polluted, is not deteriorated, is not added with any auxiliary materials, and the golden-edged leech feeding is completed within 4 hours from the time of slaughtering the blood, and the lead in the animal blood is less than or equal to 10mg/kg; cadmium is less than or equal to 0.8mg/kg; arsenic is less than or equal to 2mg/kg; mercury is less than or equal to 0.2mg/kg; copper is less than or equal to 15mg/kg, and the animal blood does not contain Bdellovibrio pseudomonad, aeromonas hydrophila and aeromonas maculosa;
3) In the cultivation process, the golden-edged leeches are placed in gentamicin solution with 8 ten thousand units per 100mL for 2 hours every two weeks, and then water is changed for cleaning;
4) Stopping feeding 2 months before sale, and immersing and washing the golden-edged leeches in 0.02% chlorhexidine water solution for 10 minutes before sale, thus completing the cleaning and purifying of golden-edged leech saliva.
Example 10
A method for cleaning and purifying living golden-edged leech saliva comprises the following steps:
1) Adding cultivation water into a large-mouth glass bottle, and placing the golden-edged leech larvae into a cultivation device for cultivation; wherein, the water for cultivation meets the specified requirement of fishery water quality standard (GB 11607), and the pH value of the water body is 6.5; as shown in fig. 2, the cultivation device includes:
the cross section of the cultivation box 1 is rectangular, the cultivation box 1 is made of transparent materials, a rectangular feeding opening 2 is arranged at the top of the cultivation box 1 along the width direction of the cultivation box, a drain outlet 3 corresponding to the feeding opening 2 is arranged at the bottom of the cultivation box 1, first convex edges are arranged on the left side and the right side of the drain outlet 3 at the bottom of the cultivation box 1, water baffles are arranged on the left side and the right side of the drain outlet 3, the water baffles are respectively hinged to the bottom of the cultivation box through hinges, when the water baffles on the left side and the right side rotate to the position vertical to the bottom of the cultivation box 1, the lower ends of the water baffles on the left side and the right side are abutted against the first convex edges, a connecting lug is arranged at one end of the water baffles on the left side and the right side, which is far away from the hinged position, a positioning plate 5 is arranged below the feed inlet 2, an electric telescopic rod 6 with a waterproof function is arranged between the water baffles at the left side and the right side, one end of the electric telescopic rod 6 is hinged with a connecting lug on the water baffle at the left side, the other end of the electric telescopic rod 6 is hinged with a connecting lug on the water baffle at the right side 7, second convex edges are arranged on the inner side walls of the front side and the rear side of the box body of the cultivation box 1, the basic length of the electric telescopic rod 6 is smaller than the width of the sewage outlet 3 so that the two sides of the water baffle are in butt joint with the second convex edges when the electric telescopic rod 6 contracts, the top of the water baffle is in butt joint with the positioning plate 5, the bottom of the water baffle is in butt joint with the first convex edges, and the water baffles at the left side and the right side and the side wall and the bottom of the cultivation box 1 are enclosed into a feeding area with rectangular cross section; the sewage outlet 3 is provided with an escape preventing net, a valve switch is arranged below the escape preventing net to open or close the sewage outlet 3, and an escape preventing device is arranged at the feeding port 2; the edges of the water baffles on the left side and the right side are provided with sealing strips so that when the water baffles are pulled up by the electric telescopic rod 6, the edges of the water baffles on the left side and the right side are tightly connected with the first convex edge and the second convex edge; the bottoms of the culture boxes 1 on the two sides of the sewage outlet 3 are inclined to form an angle of 10 degrees with the horizontal ground. The bottom plates of the cultivation boxes 1 at the two sides of the sewage outlet 3 are obliquely arranged with the horizontal ground, so that the water baffle plates are also inclined at ordinary times, an included angle is formed between the left water baffle plate 4 and the right water baffle plate 7, and when the electric telescopic rod 6 is contracted, the left water baffle plate 4 and the right water baffle plate 7 are pulled up more easily;
2) Collecting fresh and clean duck blood, filling an artificial casing, and then filling the artificial casing into a feeding device to feed the golden-edged leeches, wherein the animal blood is not polluted, is not deteriorated, is not added with any auxiliary materials, and the golden-edged leech feeding is completed within 4 hours from the time of slaughtering the blood, and the lead in the animal blood is less than or equal to 10mg/kg; cadmium is less than or equal to 0.8mg/kg; arsenic is less than or equal to 2mg/kg; mercury is less than or equal to 0.2mg/kg; copper is less than or equal to 15mg/kg, and the animal blood does not contain Bdellovibrio pseudomonad, aeromonas hydrophila and aeromonas maculosa;
3) In the cultivation process, the golden-edged leeches are placed in gentamicin solution with 8 ten thousand units per 100mL for 2 hours every two weeks, and then water is changed for cleaning;
4) Stopping feeding 2 months before sale, and immersing and washing the golden-edged leeches in 0.02% chlorhexidine water solution for 10 minutes before sale, thus completing the cleaning and purifying of golden-edged leech saliva.
Experimental group: the leeches cultivated by the cultivation methods of examples 1-10 were subjected to pathogen detection, the method was referred to the pathogenic aeromonas hydrophila detection method (GB/T18652-2002), whether the leeches were contained pseudomonas and aeromonas hydrophila was detected, and the detection results are shown in Table 1.
Control group: leeches cultivated by the cultivation method according to examples 1-10, but using sterilized distilled water instead of gentamicin solution and chlorhexidine solution, the pathogenic bacteria of the leeches obtained by cultivation were detected, the method according to the pathogenic aeromonas hydrophila detection method (GB/T18652-2002), whether leech-containing pseudomonas and aeromonas hydrophila were detected, and the detection results are shown in Table 1.
TABLE 1
Note that: the difference compared with the control group has very significant meaning (P < 0.01)
Although embodiments of the invention have been disclosed above, they are not limited to the use listed in the specification and embodiments. It can be applied to various fields suitable for the present invention. Additional modifications will readily occur to those skilled in the art. Therefore, the invention is not to be limited to the specific details and illustrations shown and described herein, without departing from the general concepts defined in the claims and their equivalents.
Claims (9)
1. The method for cleaning and purifying living golden-edged leech saliva is characterized by comprising the following steps of:
1) Adding water for cultivation into a cultivation device, and placing the golden-edged leech larvae into the cultivation device for cultivation;
the cultivation device comprises:
the cultivation box is a box body with a rectangular cross section, the cultivation box is made of transparent materials, a rectangular feeding port is arranged at the top of the cultivation box along the width direction of the cultivation box, a drain port corresponding to the feeding port is arranged at the bottom of the cultivation box, first convex edges are arranged at the left side and the right side of the drain port at the bottom of the cultivation box, water baffles are arranged at the left side and the right side of the drain port, when the water baffles are hinged at the bottom of the cultivation box through hinges so that the water baffles rotate to be perpendicular to the bottom of the cultivation box, the lower ends of the water baffles are in butt joint with the first convex edges, connecting lugs are arranged at the other ends of the water baffles, which are far away from the hinged positions, of the feeding port are provided with positioning plates, one end of each electric telescopic rod is hinged with the connecting lug on the left side of the corresponding water baffle, the other end of each electric telescopic rod is hinged with the connecting lug on the right side of the corresponding water baffle, second convex edges are arranged on the inner side walls of the front side and the rear side of the box, when the width of each electric telescopic rod is smaller than the width of the water baffles, and the width of the water baffles are in butt joint with the first side walls of the rectangular base, and the two sides of the water baffles are in butt joint with the rectangular side walls, and the width of the water baffles are in butt joint with the bottom of the rectangular side walls, and the two sides of the water baffles, and the bottom of the rectangular side walls are in butt joint with the side walls, and the front side of the water baffles and the front and the side and the bottom and the side wall is connected with the two side wall and the sealing plate;
2) Collecting fresh and clean animal blood, filling an artificial casing, and then feeding the leeches with golden edges in a feeding device for continuous cultivation;
3) In the cultivation process, the golden-edged leeches are placed in gentamicin solution with 8-12 ten thousand units per 100mL for 2-4 hours every two weeks, and then water is changed for cleaning;
4) Stopping feeding 2-3 months before sale, and soaking and washing the leech with 0.02-0.1% chlorhexidine water solution for 10-20min before sale to finish cleaning and purifying the leech saliva.
2. The method for cleaning and purifying live golden-edged leech saliva as claimed in claim 1, wherein the water for cultivation meets the requirements specified in fishery water quality standard, and the pH value of the water is 6.5-7.0.
3. The method for cleaning and purifying live golden-edged leech saliva as recited in claim 1, wherein the animal blood is chicken, duck, pig, cow, sheep blood.
4. The method for cleaning and purifying live golden-edged leech saliva as claimed in claim 3, wherein the animal blood is required to be free from pollution, deterioration and addition of any auxiliary materials, and golden-edged leech feeding should be completed within 4 hours from the time of slaughtering blood.
5. The method for cleaning and purifying live golden-edged leech saliva as recited in claim 1, wherein lead in the animal blood is less than or equal to 10mg/kg; cadmium is less than or equal to 0.8mg/kg; arsenic is less than or equal to 2mg/kg; mercury is less than or equal to 0.2mg/kg; copper is less than or equal to 15mg/kg.
6. The method for cleaning and purifying live golden-edged leech saliva as recited in claim 1, wherein the animal blood does not contain leech pseudomonas, aeromonas hydrophila or aeromonas maculosa.
7. The method for cleaning and purifying live golden-edged leech saliva as recited in claim 1, wherein the sewage outlet is provided with an escape-proof net, a valve switch is arranged below the escape-proof net to open or close the sewage outlet, and an escape-proof device is arranged at the feed inlet.
8. The method for cleaning and purifying live golden-edged leech saliva as recited in claim 1, wherein sealing strips are arranged at edges of the left and right water baffles so that the edges of the left and right water baffles are tightly connected with the first convex edge and the second convex edge when the water baffles are pulled up by the electric telescopic rod.
9. The method for cleaning and purifying live golden-edged leech saliva as recited in claim 1, wherein the bottoms of the cultivation boxes on both sides of the sewage outlet are inclined at an angle of 5-10 degrees with the horizontal ground.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201911054477.7A CN110710496B (en) | 2019-10-31 | 2019-10-31 | Method for cleaning and purifying live golden-edged leech saliva |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201911054477.7A CN110710496B (en) | 2019-10-31 | 2019-10-31 | Method for cleaning and purifying live golden-edged leech saliva |
Publications (2)
| Publication Number | Publication Date |
|---|---|
| CN110710496A CN110710496A (en) | 2020-01-21 |
| CN110710496B true CN110710496B (en) | 2024-01-23 |
Family
ID=69213563
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN201911054477.7A Active CN110710496B (en) | 2019-10-31 | 2019-10-31 | Method for cleaning and purifying live golden-edged leech saliva |
Country Status (1)
| Country | Link |
|---|---|
| CN (1) | CN110710496B (en) |
Families Citing this family (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN114514899B (en) * | 2022-02-25 | 2023-03-10 | 广西蛭郎中生物科技有限公司 | Purification cultivation method of Guangxi golden-edged leeches |
Citations (7)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| JP2002223661A (en) * | 2001-01-30 | 2002-08-13 | Kajima Corp | Rearing equipment for fish-and-shellfish |
| JP2002272311A (en) * | 2001-03-22 | 2002-09-24 | Masato Kino | Method for feeding liquid feed for fish and shellfish |
| CN103782958A (en) * | 2014-02-21 | 2014-05-14 | 龙南源头活水生态科技有限责任公司 | Imitative-ecological one-button factory breeding and domesticating method for frogs |
| CN104430190A (en) * | 2014-10-29 | 2015-03-25 | 南宁市净雪皇生物工程有限公司 | Device and method for feeding poecilobdella manillensis manually |
| CN105684996A (en) * | 2016-02-05 | 2016-06-22 | 吴铁明 | Method for breeding medical blood sucking leeches |
| CN109452205A (en) * | 2019-01-17 | 2019-03-12 | 云南茂湾水产养殖有限责任公司 | A kind of anti-unrestrained fish culture method of scale |
| CN209017684U (en) * | 2018-10-26 | 2019-06-25 | 大连超熙渔业科技开发有限公司 | Sewage changes water-bound in a kind of aquaculture tank |
-
2019
- 2019-10-31 CN CN201911054477.7A patent/CN110710496B/en active Active
Patent Citations (7)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| JP2002223661A (en) * | 2001-01-30 | 2002-08-13 | Kajima Corp | Rearing equipment for fish-and-shellfish |
| JP2002272311A (en) * | 2001-03-22 | 2002-09-24 | Masato Kino | Method for feeding liquid feed for fish and shellfish |
| CN103782958A (en) * | 2014-02-21 | 2014-05-14 | 龙南源头活水生态科技有限责任公司 | Imitative-ecological one-button factory breeding and domesticating method for frogs |
| CN104430190A (en) * | 2014-10-29 | 2015-03-25 | 南宁市净雪皇生物工程有限公司 | Device and method for feeding poecilobdella manillensis manually |
| CN105684996A (en) * | 2016-02-05 | 2016-06-22 | 吴铁明 | Method for breeding medical blood sucking leeches |
| CN209017684U (en) * | 2018-10-26 | 2019-06-25 | 大连超熙渔业科技开发有限公司 | Sewage changes water-bound in a kind of aquaculture tank |
| CN109452205A (en) * | 2019-01-17 | 2019-03-12 | 云南茂湾水产养殖有限责任公司 | A kind of anti-unrestrained fish culture method of scale |
Also Published As
| Publication number | Publication date |
|---|---|
| CN110710496A (en) | 2020-01-21 |
Similar Documents
| Publication | Publication Date | Title |
|---|---|---|
| CN110710496B (en) | Method for cleaning and purifying live golden-edged leech saliva | |
| CN206118354U (en) | Degassing unit of peppery wooden seed | |
| CN106942096A (en) | A kind of many squamas shovel the artificial propagation breeding method of jaw fish | |
| CN104041483A (en) | Preparation method of miniature marine animal plastinated specimens | |
| Obst | A bacteriologic study of sardines | |
| CN106577354A (en) | Scale-breeding chicken farm disinfection treatment method | |
| CN104982384A (en) | Toad cortex artificial collection method and toad cortex separator | |
| CN220023810U (en) | Peculiar smell removing device for animal husbandry cultivation | |
| CN207167380U (en) | Herding reclaims crib with feed | |
| CN213881391U (en) | Batch production device for in-vitro eggs of red swamp crayfish | |
| CN206196670U (en) | A kind of clam worm biological feedstuff culture pond | |
| CN209788204U (en) | Eel is bred and uses faecal management device | |
| CN107517867A (en) | A kind of Modern Animal Husbandry cultivation drinking method | |
| CN207589898U (en) | A kind of device of automatic collection black soldier flies pupa | |
| CN108668984B (en) | Tilapia fry high-density transportation method | |
| CN215712438U (en) | Live pig is bred with plant sewage treatment plant | |
| CN217407354U (en) | Excrement cleaning device for black pig breeding fermentation bed | |
| CN219939541U (en) | Automatic scale-removing machine | |
| CN217012344U (en) | Artificial incubator of mantis shrimp at incubation port of cement pond of factory workshop | |
| CN213416852U (en) | Ecological treatment device for cultivation biomass waste | |
| CN220897637U (en) | Frog type polymerization growth disinfection and sterilization device | |
| CN207784034U (en) | A kind of lucilia sericata is cultivated and breeding apparatus | |
| CN217546950U (en) | Animal experiment is with raising cage convenient to wash | |
| CN207159022U (en) | Quality purifying device for water | |
| CN218089066U (en) | Oyster is raised temporarily and is purified circulation and use sea water treatment and sterilization equipment |
Legal Events
| Date | Code | Title | Description |
|---|---|---|---|
| PB01 | Publication | ||
| PB01 | Publication | ||
| SE01 | Entry into force of request for substantive examination | ||
| SE01 | Entry into force of request for substantive examination | ||
| DD01 | Delivery of document by public notice |
Addressee: Luo Zhongbu Document name: Notice of Termination of Procedure |
|
| DD01 | Delivery of document by public notice | ||
| GR01 | Patent grant | ||
| GR01 | Patent grant |