CN110699850B - Preparation method of polyhydroxyalkanoate/polypyrrole composite electrospinning membrane and electrospinning membrane - Google Patents
Preparation method of polyhydroxyalkanoate/polypyrrole composite electrospinning membrane and electrospinning membrane Download PDFInfo
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- D—TEXTILES; PAPER
- D04—BRAIDING; LACE-MAKING; KNITTING; TRIMMINGS; NON-WOVEN FABRICS
- D04H—MAKING TEXTILE FABRICS, e.g. FROM FIBRES OR FILAMENTARY MATERIAL; FABRICS MADE BY SUCH PROCESSES OR APPARATUS, e.g. FELTS, NON-WOVEN FABRICS; COTTON-WOOL; WADDING ; NON-WOVEN FABRICS FROM STAPLE FIBRES, FILAMENTS OR YARNS, BONDED WITH AT LEAST ONE WEB-LIKE MATERIAL DURING THEIR CONSOLIDATION
- D04H1/00—Non-woven fabrics formed wholly or mainly of staple fibres or like relatively short fibres
- D04H1/40—Non-woven fabrics formed wholly or mainly of staple fibres or like relatively short fibres from fleeces or layers composed of fibres without existing or potential cohesive properties
- D04H1/42—Non-woven fabrics formed wholly or mainly of staple fibres or like relatively short fibres from fleeces or layers composed of fibres without existing or potential cohesive properties characterised by the use of certain kinds of fibres insofar as this use has no preponderant influence on the consolidation of the fleece
- D04H1/4326—Condensation or reaction polymers
- D04H1/435—Polyesters
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- D01—NATURAL OR MAN-MADE THREADS OR FIBRES; SPINNING
- D01D—MECHANICAL METHODS OR APPARATUS IN THE MANUFACTURE OF ARTIFICIAL FILAMENTS, THREADS, FIBRES, BRISTLES OR RIBBONS
- D01D5/00—Formation of filaments, threads, or the like
- D01D5/0007—Electro-spinning
- D01D5/0015—Electro-spinning characterised by the initial state of the material
- D01D5/003—Electro-spinning characterised by the initial state of the material the material being a polymer solution or dispersion
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- D01—NATURAL OR MAN-MADE THREADS OR FIBRES; SPINNING
- D01D—MECHANICAL METHODS OR APPARATUS IN THE MANUFACTURE OF ARTIFICIAL FILAMENTS, THREADS, FIBRES, BRISTLES OR RIBBONS
- D01D5/00—Formation of filaments, threads, or the like
- D01D5/0007—Electro-spinning
- D01D5/0061—Electro-spinning characterised by the electro-spinning apparatus
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- D—TEXTILES; PAPER
- D01—NATURAL OR MAN-MADE THREADS OR FIBRES; SPINNING
- D01D—MECHANICAL METHODS OR APPARATUS IN THE MANUFACTURE OF ARTIFICIAL FILAMENTS, THREADS, FIBRES, BRISTLES OR RIBBONS
- D01D5/00—Formation of filaments, threads, or the like
- D01D5/0007—Electro-spinning
- D01D5/0061—Electro-spinning characterised by the electro-spinning apparatus
- D01D5/0092—Electro-spinning characterised by the electro-spinning apparatus characterised by the electrical field, e.g. combined with a magnetic fields, using biased or alternating fields
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- D—TEXTILES; PAPER
- D01—NATURAL OR MAN-MADE THREADS OR FIBRES; SPINNING
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- D01F6/00—Monocomponent artificial filaments or the like of synthetic polymers; Manufacture thereof
- D01F6/88—Monocomponent artificial filaments or the like of synthetic polymers; Manufacture thereof from mixtures of polycondensation products as major constituent with other polymers or low-molecular-weight compounds
- D01F6/92—Monocomponent artificial filaments or the like of synthetic polymers; Manufacture thereof from mixtures of polycondensation products as major constituent with other polymers or low-molecular-weight compounds of polyesters
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- D06—TREATMENT OF TEXTILES OR THE LIKE; LAUNDERING; FLEXIBLE MATERIALS NOT OTHERWISE PROVIDED FOR
- D06B—TREATING TEXTILE MATERIALS USING LIQUIDS, GASES OR VAPOURS
- D06B13/00—Treatment of textile materials with liquids, gases or vapours with aid of vibration
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- D06M15/00—Treating fibres, threads, yarns, fabrics, or fibrous goods made from such materials, with macromolecular compounds; Such treatment combined with mechanical treatment
- D06M15/19—Treating fibres, threads, yarns, fabrics, or fibrous goods made from such materials, with macromolecular compounds; Such treatment combined with mechanical treatment with synthetic macromolecular compounds
- D06M15/37—Macromolecular compounds obtained otherwise than by reactions only involving carbon-to-carbon unsaturated bonds
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- D—TEXTILES; PAPER
- D06—TREATMENT OF TEXTILES OR THE LIKE; LAUNDERING; FLEXIBLE MATERIALS NOT OTHERWISE PROVIDED FOR
- D06M—TREATMENT, NOT PROVIDED FOR ELSEWHERE IN CLASS D06, OF FIBRES, THREADS, YARNS, FABRICS, FEATHERS OR FIBROUS GOODS MADE FROM SUCH MATERIALS
- D06M2101/00—Chemical constitution of the fibres, threads, yarns, fabrics or fibrous goods made from such materials, to be treated
- D06M2101/16—Synthetic fibres, other than mineral fibres
- D06M2101/30—Synthetic polymers consisting of macromolecular compounds obtained otherwise than by reactions only involving carbon-to-carbon unsaturated bonds
- D06M2101/32—Polyesters
Abstract
The invention discloses a preparation method of a polyhydroxyalkanoate/polypyrrole composite electrospun membrane and the electrospun membrane. The preparation method is simple and rapid, raw materials are easy to obtain, the prepared polyhydroxyalkanoate/polypyrrole composite electrospun membrane has good hydrophilicity and cell compatibility, can provide a durable and stable skeleton structure support after being implanted into tissues, can regulate the attachment and migration of cells under electrical stimulation and induce tissue differentiation, and has wide application prospects in the field of bioengineering such as tissue directional repair and the like.
Description
Technical Field
The invention relates to the field of medical biomaterials, in particular to a preparation method of a polyhydroxyalkanoate/polypyrrole composite electrospun membrane.
Background
Electrostatic spinning is a special fiber manufacturing process, and polymer solution is subjected to jet spinning under the action of a strong electric field to obtain nano-scale fiber filaments. The fiber structure can simulate the structure and biological function of natural extracellular matrix, and potential applications include drug-loaded scaffolds, tissue and organ repair scaffolds and the like. However, the polymer molecules available for electrostatic spinning are limited in their types, their structures are easily damaged by external forces, and their mechanical properties and biocompatibility are often unsatisfactory.
In recent years, a class of high polymer materials obtained by microbial fermentation attracts great attention, namely Polyhydroxyalkanoates (PHA), the PHA is completely synthesized by microorganisms, the process is simple and green, the degradation period is as long as 9-12 months, and the PHA has good controllable degradability, biocompatibility and hot-working performance. The structure is diversified, and different material properties can be obtained according to different arrangement combination modes and chain lengths of monomers. But the material is insoluble in water, the conventional forming process is difficult to realize, and ideal nano-scale fiber yarns can be obtained by matching the organic solvent with the electrostatic spinning process after being dissolved. The prior patent technology discloses a polyhydroxybutyrate/polypyrrole composite conductive nanofiber membrane, but the preparation method is relatively complex, and the properties of the obtained polyhydroxybutyrate/polypyrrole composite conductive nanofiber membrane cannot be determined.
Disclosure of Invention
The invention aims to overcome at least one defect of the prior art and provides a novel preparation method of the polyhydroxyalkanoate/polypyrrole composite electrospun membrane, which is simple and rapid, and has easily obtained raw materials.
Another object of the present invention is to provide a polyhydroxyalkanoate/polypyrrole composite electrospun membrane.
The technical scheme adopted by the invention is as follows:
a preparation method of a polyhydroxyalkanoate/polypyrrole composite electrospinning film comprises the following steps:
s1, preparing a polyhydroxyalkanoate nanofiber electrospun membrane: dissolving polyhydroxyalkanoate powder in a dichloromethane and dimethylformamide composite solvent, uniformly stirring to prepare polyhydroxyalkanoate solution with the concentration of 90-100 mg/mL, adding the polyhydroxyalkanoate solution into an injector, fixing the injector by using a needle nozzle, setting the distance between a needle and a receiver to be 14-16 cm at room temperature, the voltage to be 12-18 kilovolts, the advancing speed of the injector to be 2.8-3.2 mL/h, collecting electrospinning through an ethanol solution, washing the obtained electrospinning with deionized water, carrying out vacuum freeze drying overnight to obtain polyhydroxyalkanoate nanofibers, and carrying out hot pressing and film forming on the nanofibers to obtain a polyhydroxyalkanoate electrospinning film;
s2, preparing a polyhydroxyalkanoate/polypyrrole composite electrospinning film: uniformly stirring 1-5 g of ferric trichloride and 50-100 mL of deionized water, uniformly mixing 0.1-0.5 g of pyrrole and 50-100 mL of deionized water, uniformly mixing two solutions of ferric trichloride solution and pyrrole solution to obtain a mixed solution, placing the polyhydroxyalkanoate electrospun membrane into the mixed solution at room temperature, ultrasonically vibrating for 0.5-12 h, taking out, washing a sample with deionized water for 3-5 times, and carrying out vacuum freeze drying to obtain the polyhydroxyalkanoate/polypyrrole composite electrospun membrane.
The preparation method of the polyhydroxyalkanoate/polypyrrole composite electrospinning film is characterized in that the polyhydroxyalkanoate fiber electrospinning film is prepared through an electrostatic spinning process, and then the polyhydroxyalkanoate/polypyrrole composite electrospinning film is prepared through a surface modification technology, wherein polypyrrole uniformly covers the surface of a polyhydroxyalkanoate network structure, so that the appearance is smooth, the microstructure is closer to a natural extracellular matrix, and the cell adhesion growth is facilitated. The internal polyhydroxyalkanoate can well maintain the framework support, and the mechanical property cannot be influenced by the doping of polypyrrole. The preparation process is simpler, and the application range is high in universality.
Preferably, the concentration of the polyhydroxyalkanoate solution in step S1 is 95-105 mg/mL.
Preferably, the volume ratio of the dichloromethane to the dimethylformamide in the step S1 is (2-4): 5-9. More preferably, the volume ratio of dichloromethane to dimethylformamide is 3: 7.
Preferably, the distance between the needle and the receptacle in step S1 is 15 cm.
Preferably, the voltage in step S1 is 15 kv.
Preferably, the injector advance rate in step S1 is 3 ml/hr.
Preferably, the mass ratio of the ferric trichloride to the pyrrole in the step S2 is (13-14): 2-3.
Preferably, the ultrasonic oscillation time in step S2 is 0.5-6 h. More preferably, the ultrasonic oscillation time in step S2 is 1-3 h.
Preferably, the polyhydroxyalkanoate powder in step S1 is medical grade polyhydroxyalkanoate powder, and the purity is greater than 99.5%.
Preferably, the room temperature condition is a temperature of 20 ℃ and a humidity of 45%.
The polyhydroxyalkanoate/polypyrrole composite electrospun membrane prepared by the preparation method. The polyhydroxyalkanoate/polypyrrole composite electrospun membrane disclosed by the invention takes polyhydroxyalkanoate as a basic framework, has good mechanical property, controllable degradability and biocompatibility, and is coated with the polyhydroxyalkanoate framework, so that the composite electrospun membrane has good hydrophilicity and electrical conductivity of polypyrrole, and the prepared polyhydroxyalkanoate/polypyrrole composite electrospun membrane has the excellent properties of polyhydroxyalkanoate and polypyrrole at the same time, the appearance of the obtained polyhydroxyalkanoate/polypyrrole composite electrospun membrane is smooth and flat membrane, the microstructure of the obtained polyhydroxyalkanoate/polypyrrole composite electrospun membrane is similar to that of a natural extracellular matrix, the mechanical property and the biocompatibility are excellent, a durable and stable framework structure support can be provided after the membrane is implanted into tissues, the degradation period is as long as 9-12 months, and the cell adhesion can be adjusted under electrical stimulation, Migration and tissue differentiation induction, and has wide application prospect in the field of bioengineering such as tissue directional repair and the like.
Compared with the prior art, the invention has the beneficial effects that: the preparation method of the invention is that firstly preparing the polyhydroxyalkanoate fiber electrospinning film by an electrostatic spinning process, and then preparing the polyhydroxyalkanoate/polypyrrole composite electrospinning film by a surface modification technology, wherein polypyrrole is uniformly covered on the surface of a polyhydroxyalkanoate network structure, the appearance is smooth, the microstructure is closer to the natural extracellular matrix, and the cell adhesion growth is facilitated; the polyhydroxyalkanoate/polypyrrole composite electrospun membrane prepared by the invention has the excellent performances of polyhydroxyalkanoate and polypyrrole, has excellent mechanical property and biocompatibility, can provide a durable and stable skeleton structure support after being implanted into tissues, has a degradation period of 9-12 months, can regulate the attachment and migration of cells under electric stimulation, induces tissue differentiation, and has wide application prospects in the field of bioengineering such as tissue directional repair and the like; the preparation process is simpler and has high universality in application range.
Drawings
FIG. 1 is an appearance view and a scanning electron microscope image of an electrospun membrane of Polyhydroxyalkanoate (PHA) in example 1.
FIG. 2 is an appearance diagram and a scanning electron microscope diagram of the polyhydroxyalkanoate/polypyrrole (PHA/PPY) composite electrospun membrane of example 1.
FIG. 3 is a bar graph of the cell proliferation activity of the PHA electrospun membranes of example 1 and the PHA/PPY composite electrospun membranes.
FIG. 4 is a photograph of immunofluorescent staining of cells of PHA electrospun membranes and PHA/PPY composite electrospun membranes of example 1.
Detailed Description
The drawings are only for purposes of illustration and are not to be construed as limiting the invention. For a better understanding of the following embodiments, certain features of the drawings may be omitted, enlarged or reduced, and do not represent the size of an actual product; it will be understood by those skilled in the art that certain well-known structures in the drawings and descriptions thereof may be omitted.
Example 1
A polyhydroxyalkanoate/polypyrrole composite electrospinning membrane is prepared by the following steps:
a preparation method of a polyhydroxyalkanoate/polypyrrole composite electrospinning film comprises the following steps:
s1, preparation of a Polyhydroxyalkanoate (PHA) nanofiber electrospun membrane: weighing 1g of medical-grade PHA powder, dissolving in 10mL of a dichloromethane-dimethylformamide composite solvent (the volume ratio of dichloromethane to dimethylformamide is 3:7), uniformly stirring to prepare 100mg/mL PHA solution, adding the PHA solution into a 1mL injector, and fixing the injector by using a needle nozzle. The distance between the needle and the receiver was set at 15cm at room temperature, the voltage was 15 kv, the syringe advancing speed was 3.0 ml/h, and the electrospun fiber was collected by the ethanol solution. Subsequently, the obtained electrospun fiber was washed with deionized water 3 times, and freeze-dried overnight to obtain polyhydroxyalkanoate nanofibers. And (3) carrying out hot pressing on the nano fibers to form a film so as to obtain the PHA electrospun film.
S2, preparation of polyhydroxyalkanoate/polypyrrole (PHA/PPY) composite electrospun membrane: 1.36g of ferric trichloride was weighed into a 100mL beaker, 100mL of deionized water was added and stirred well, then 0.244g of pyrrole was weighed into another 100mL of deionized water and the two solutions 1:1 were mixed well. And (3) placing the polyhydroxyalkanoate electrospun membrane into the mixed solution at room temperature, ultrasonically vibrating for 1h, taking out, washing the sample with deionized water for 3 times, and carrying out vacuum freeze drying to obtain the polyhydroxyalkanoate/polypyrrole composite electrospun membrane.
Example 2
A preparation method of a polyhydroxyalkanoate/polypyrrole composite electrospinning film comprises the following steps:
s1, preparation of a Polyhydroxyalkanoate (PHA) nanofiber electrospun membrane: 0.95g of medical-grade PHA powder is weighed and dissolved in 10mL of dichloromethane-dimethylformamide composite solvent (the volume ratio of dichloromethane to dimethylformamide is 3:7), uniformly stirred to prepare 100mg/mL PHA solution, and the PHA solution is added into a 1mL injector which is fixed by a needle nozzle. The distance between the needle and the receiver was set at 15cm at room temperature, the voltage was 15 kv, the syringe advancing speed was 3.0 ml/hr, and the electrospun fiber was collected by the ethanol solution. Subsequently, the obtained electrospun fiber was washed with deionized water 3 times, and freeze-dried overnight to obtain polyhydroxyalkanoate nanofibers. And (3) carrying out hot pressing on the nano fibers to form a film so as to obtain the PHA electrospun film.
S2, preparation of polyhydroxyalkanoate/polypyrrole (PHA/PPY) composite electrospun membrane: 1.30g of ferric trichloride is weighed into a 100mL beaker, 100mL of deionized water is added and stirred uniformly, then 0.23g of pyrrole is weighed and dissolved in another 100mL of deionized water, and the two solutions are mixed uniformly in a ratio of 1: 1. And (3) placing the polyhydroxyalkanoate electrospun membrane into the mixed solution at room temperature, carrying out ultrasonic oscillation for 2h, taking out, washing the sample with deionized water for 3 times, and carrying out vacuum freeze drying to obtain the polyhydroxyalkanoate/polypyrrole composite electrospun membrane.
Example 3
A preparation method of a polyhydroxyalkanoate/polypyrrole composite electrospinning film comprises the following steps:
s1, preparation of a Polyhydroxyalkanoate (PHA) nanofiber electrospun membrane: weighing 1.05g of medical-grade PHA powder, dissolving in 10mL of a composite solvent of dichloromethane and dimethylformamide (the volume ratio of dichloromethane to dimethylformamide is 3:7), uniformly stirring to prepare 100mg/mL PHA solution, adding the PHA solution into a 1mL injector, and fixing the injector by using a needle nozzle. The distance between the needle and the receiver was set at 15cm at room temperature, the voltage was 15 kv, the syringe advancing speed was 3.0 ml/hr, and the electrospun fiber was collected by the ethanol solution. Subsequently, the obtained electrospun fiber was washed with deionized water 3 times, and freeze-dried overnight to obtain polyhydroxyalkanoate nanofibers. And (3) carrying out hot pressing on the nano fibers to form a film so as to obtain the PHA electrospun film.
S2, preparation of polyhydroxyalkanoate/polypyrrole (PHA/PPY) composite electrospun membrane: 1.40g of ferric trichloride is weighed into a 100mL beaker, 100mL of deionized water is added and stirred uniformly, then 0.25g of pyrrole is weighed and dissolved in another 100mL of deionized water, and the two solutions are mixed uniformly in a ratio of 1: 1. And (3) placing the polyhydroxyalkanoate electrospun membrane into the mixed solution at room temperature, carrying out ultrasonic oscillation for 3h, taking out, washing the sample with deionized water for 3 times, and carrying out vacuum freeze drying to obtain the polyhydroxyalkanoate/polypyrrole composite electrospun membrane.
In order to verify whether the PHA/PPY composite electrospun membranes were successfully produced, physical appearances and SEM images of the PHA electrospun membranes and the PHA/PPY composite electrospun membranes of examples 1 to 3 were compared and tested. The comparison of the appearance of the substance and a scanning electron microscope image shows that compared with the PHA electrospun membrane, the prepared PHA/PPY composite electrospun membrane has changed appearance form, the color is changed from white to black, and the appearance form is changed to be smoother and smoother; under SEM micrographs, the smooth and porous fiber structure surface is successfully coated with ultrathin substances, the fiber diameter is obviously increased, the pore structure is coated, the structure of the pore structure is close to the natural extracellular matrix, the cell growth environment can be simulated, and the cell proliferation and growth can be promoted to promote the tissue directed repair and regeneration. FIG. 1 shows the appearance and SEM images of the PHA electrospun membrane of example 1; FIG. 2 is an appearance view and a scanning electron microscope image of the PHA/PPY composite electrospun membrane of example 1.
For further validation of PHA/PPY composite electrospinningThe invention evaluates the influence of the electrospun membrane on cell proliferation through a CCK-8 cytotoxic test. Cutting the PHA electrospun membrane and the PHA/PPY composite electrospun membrane to a proper size (a circle with an area of about 0.32 square centimeter), placing the membrane at the bottom of a 96-well plate after ethylene oxide sterilization, and pre-culturing the membrane in a cell culture medium overnight. Adjusting mesenchymal stem cell density to 1 × 105Per mL, adding 200 mu L of cell sap into each hole, and co-culturing overnight until the cells recover to a normal adherent state; adding 20uL CCK-8 reagent at different time points (day 1, day 3 and day 5) respectively for incubation for 2 h; the supernatant was then transferred to a new 96-well plate, the absorbance value of the solution was measured by a microplate reader at 450nm, and the cell proliferation activity percentage was calculated. As shown in fig. 3, which is a bar graph of cell proliferation activity of the PHA electrospun membrane and the PHA/PPY composite electrospun membrane in example 1, it can be seen from the graph that the cell proliferation activity of the PHA/PPY composite electrospun membrane in days 1, 3, and 5 exceeds 80%, which is significantly higher than that of the PHA electrospun membrane, which proves that the PHA/PPY composite electrospun membrane has a good promotion effect on cell proliferation, and the PPY coating improves the defect of hydrophobic PHA electrospun membrane, and can better promote cell proliferation and differentiation.
Furthermore, the invention also observes the cell proliferation state through cell immunofluorescence staining, and the specific experimental steps are as follows: cutting PHA electrospun membrane and PHA/PPY composite electrospun membrane to proper size, sterilizing with ethylene oxide, placing at the bottom of 48-pore plate, and adjusting the density of mesenchymal stem cells to 1 × 1051mL of cell sap is added into each hole, and the cells are cultured for 3 days; the culture solution was aspirated away, and the cells were washed 3 times with PBS; fixing with 4% paraformaldehyde solution on ice for 15min, and washing with PBS for 3 times; permeabilize cells with 0.5% Triton X-100 in PBS for 10min at room temperature, followed by 3 washes with PBS; adding 5 mu L F-actin phalloidin working solution into each well, incubating for 30min at room temperature in dark place for staining, and then washing for 3 times by PBS; finally, 4-5 drops of ready-to-use DAPI solution are added into each hole, after counterstaining is carried out for 30s, excessive water is absorbed, and observation is carried out under a fluorescence microscope. FIG. 4 is a photograph of the cell immunofluorescent staining of the PHA electrospun membrane and the PHA/PPY composite electrospun membrane of example 1, from which it can be seen that the number of cell proliferations on the PHA/PPY composite electrospun membrane is significantly greater than that of the PHA electrospun membrane and the blank control group,the cytoskeleton and the nucleus structure are clear, and the PHA/PPY composite electrospun membrane is proved to have good promotion effect on cell proliferation and differentiation.
It should be understood that the above-mentioned embodiments of the present invention are only examples for clearly illustrating the technical solutions of the present invention, and are not intended to limit the specific embodiments of the present invention. Any modification, equivalent replacement, and improvement made within the spirit and principle of the present invention claims should be included in the protection scope of the present invention claims.
Claims (10)
1. A preparation method of a polyhydroxyalkanoate/polypyrrole composite electrospun membrane is characterized by comprising the following steps:
s1, preparing a polyhydroxyalkanoate nanofiber electrospun membrane: dissolving polyhydroxyalkanoate powder in a dichloromethane and dimethylformamide composite solvent, uniformly stirring to prepare polyhydroxyalkanoate solution with the concentration of 90-100 mg/mL, adding the polyhydroxyalkanoate solution into an injector, fixing the injector by using a needle nozzle, setting the distance between a needle and a receiver to be 14-16 cm at room temperature, the voltage to be 12-18 kilovolts, the advancing speed of the injector to be 2.8-3.2 mL/h, collecting electrospinning through an ethanol solution, washing the obtained electrospinning with deionized water, freezing and drying in vacuum overnight to obtain polyhydroxyalkanoate nanofibers, and carrying out hot pressing film forming on the nanofibers to obtain polyhydroxyalkanoate electrospinning films;
s2, preparing a polyhydroxyalkanoate/polypyrrole composite electrospinning film: uniformly stirring 1-5 g of ferric trichloride and 50-100 mL of deionized water, uniformly mixing 0.1-0.5 g of pyrrole and 50-100 mL of deionized water, uniformly mixing two solutions of ferric trichloride solution and pyrrole solution to obtain a mixed solution, placing the polyhydroxyalkanoate electrospun membrane into the mixed solution under the condition of room temperature, ultrasonically vibrating for 0.5-12 h, taking out, washing a sample with deionized water for 3-5 times, and carrying out vacuum freeze drying to obtain the polyhydroxyalkanoate/polypyrrole composite electrospun membrane.
2. The method according to claim 1, wherein the concentration of the polyhydroxyalkanoate solution in step S1 is 95-105 mg/mL.
3. The method according to claim 1, wherein the volume ratio of dichloromethane to dimethylformamide in step S1 is (2-4): 5-9.
4. The method of claim 1, wherein the distance between the needle and the receptacle in step S1 is 15 cm.
5. The production method according to claim 1, wherein the voltage in step S1 is 15 kv.
6. The method of claim 1, wherein the injector advance rate in step S1 is 3 ml/hr.
7. The method according to claim 1, wherein the mass ratio of ferric trichloride to pyrrole in step S2 is (13-14): 2-3.
8. The method according to claim 1, wherein the ultrasonic oscillation time in step S2 is 0.5-6 h.
9. The method according to claim 1, wherein the polyhydroxyalkanoate powder in step S1 is medical grade polyhydroxyalkanoate powder, and has a purity of greater than 99.5%.
10. The polyhydroxyalkanoate/polypyrrole composite electrospun membrane prepared by the preparation method of any one of claims 1 to 9.
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| CN106943634A (en) * | 2017-02-10 | 2017-07-14 | 烟台正海生物科技股份有限公司 | A kind of adsorbable artificial endocranium with anti-infective function and preparation method and application |
| CN107699982A (en) * | 2017-10-31 | 2018-02-16 | 无锡中科光远生物材料有限公司 | A kind of preparation method of modified polyhydroxyalkanoate composite fibrous scaffold |
| CN108004679A (en) * | 2017-12-01 | 2018-05-08 | 苏州君康医疗科技有限公司 | Doughnut blend film and preparation method containing polyether sulfone and polyhydroxyalkanoate |
| CN110592947A (en) * | 2019-08-28 | 2019-12-20 | 中山大学附属第六医院 | Preparation method of polyhydroxyalkanoate/polydopamine composite electrospinning membrane and electrospinning membrane |
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