CN101288777A - Polycaprolactone/polyethyleneglycol tissue engineering bracket material and preparation method thereof - Google Patents
Polycaprolactone/polyethyleneglycol tissue engineering bracket material and preparation method thereof Download PDFInfo
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- CN101288777A CN101288777A CNA2008100359521A CN200810035952A CN101288777A CN 101288777 A CN101288777 A CN 101288777A CN A2008100359521 A CNA2008100359521 A CN A2008100359521A CN 200810035952 A CN200810035952 A CN 200810035952A CN 101288777 A CN101288777 A CN 101288777A
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Abstract
The invention relates to a polycaprolactone/ polyethyleneglycol tissue engineering stent material and a preparation method thereof. The components of the material include: polycaprolactone and polyethylenelycol with the proportion of 100 to 0 to 40 to 60. The preparation method includes: (1) the polycaprolactone and the polyethylenelycol with a certain proportion are dissolved in cosolvent to obtain uniform transparent solution; (2) the solution is spun into a fiber felt of nano/secondary micron grade by the technique of electrostatic spinning; (3) a vacuum oven is vacuumed to remove the residual solvent of the fiber felt; (4) the fiber felt is took out, and is overlapped and filled in the mold with a specific shape; (5) the mold is put in the vacuum oven and is took out to be cooled for the UV sterilization after being heated at the constant temperature near the blend melting temperature. The stent material system of the invention has the advantages of stable structure, uniform structure of internal holes and is mutually communicated with holes and can be used as a cartilage tissue engineering cell stent.
Description
Technical field
The invention belongs to bioengineered tissue carrier material field, particularly relating to a kind of is organizational project three-dimensional porous rack material of feedstock production and preparation method thereof with polycaprolactone and Polyethylene Glycol.
Background technology
The forfeiture or the dysfunction of tissue and organ are having a strong impact on human health, cause human diseases and death.Although traditional autotransplantation, heteroplastic transplantation and artificial succedaneum have shown excellent curative, but the immunologic rejection of its existence, donor source is limited or their application that needed drawbacks limit such as second operation, and the development of organizational project has then remedied these defectives.Organizational project is by the relevant living cells of acquisition function, plant in natural or synthetic, have on the three-dimensional rack of certain space structure, In vitro culture, be implanted in the body after a large amount of propagation of cell, can reach the purpose that forms tissue and organ with certain 26S Proteasome Structure and Function, therefore, the raw material of three-dimensional porous rack and preparation method then directly affect the successful Application of organizational project.
Polycaprolactone (PCL) relies on its favorable tissue compatibility, biological degradability and easy-formation processing characteristics to obtain using widely, but the hydrophilic of PCL is relatively poor, crystallinity is higher, degradation rate than polylactide and polylactic acid slowly many, and its degraded is at first from amorphous area irregular arrangement, unordered micromolecule chain, directly influence the length of degradation cycle, thereby limited its application.At present existing bibliographical information can adopt the method for copolymerization or blend that PCL is carried out modification, for example after PCL and polylactic acid (PLA) copolymerization, the introducing of PLA has improved the hydrophilic of PCL, increase its degradation rate (.PCL modification such as Hu Yun by way of research. material Leader .2001,15 (5): 52-54.).Three equality people Zhao have made the PCL-PEG-PCL triblock copolymer, and reach a conclusion: the introducing of PEG component has improved the hydrophilic of polymer effectively, and destroyed its crystallinity (Zhao Sanping, synthetic and the performance of .PCL-PEG-PCL block copolymers such as Feng Zengguo. functional polymer journal .2002,15 (1): 67-71.).
In recent years, existing researcher is applied to polycaprolactone in the preparation of tissue engineering bracket material, but the method that adopts is not quite similar, for example human sucrose such as S.-J.Shieh prepares shape template, then in conjunction with solvent cast/particle leaching method, make cartilage tissue engineered carrier material (the Shy-Jou Shieh of ear shape PCL and PHB material, Schinichi Terada, Joseph P.Vacanti.Tissue engineering auricular reconstruction:in vitro and in vivo studies.Biomaterials 25 (2004) 1545-1557.).People's such as domestic Yang Qing " a kind of is the tissue carrier material and preparation method thereof of raw material with the polycaprolactone " (Chinese patent application number 200610024129.1, publication number CN1810303) prepared the cartilage tissue engineered rack material of ear shape PCL material equally in conjunction with melt spinning technology and immersion method.People such as the Vacanti of Harvard University implant the mesenchymal stem cell of mouse in the support of PCL electricity spinning fibre composition, cultivate with the dynamic biological reactor.After cultivating for 4 weeks, find that cytoskeleton not only can be maintained the size and the shape of original support, and the cell of observing in the support via SEM has lamination hypertrophy and cytostromatic generation, and also find that in the tissue slice immunostaining existing newborn tissue forms, and there is the phenomenon of mineralising to produce.This shows the electrospinning silk technology of utilizing, macromolecular material can be prepared into support with nano-scale fiber, cell can on nano fiber scaffold, grow with the differentiation (Guo Jiqing. nano-structure engineering polymeric stent. become the communication .2002 of mcroorganism Science ﹠ Technology Center, 4 (2): 18-19.).And people such as domestic Jia Jun adopt method of electrostatic spinning also to prepare the PCL nanofiber, field emission scan microscopic examination shows that the fiber that obtains is the nonwoven porous network structure, diameter range is 153～612nm, the smooth homogeneous of fibre morphology, hemolytic experiment, MTT test and mucous membrane irritation experiment all illustrate the PCL electrospinning fibre have excellent biological compatibility (Jia Jun etc. the preparation of polycaprolactone electrospinning fibre and Study on biocompatibility [J]. Chinese aesthetic medicine .2007,5:22.).At present domestic still do not have patent report and adopt polycaprolactone and polyethylene glycol blending thing to prepare the organizational project three-dimensional porous rack material.And adopt polycaprolactone and Polyethylene Glycol is raw material, and the method for preparing tissue engineering bracket material by electrostatic spinning technique and fiber sintering method does not all have similar or similar patent report both at home and abroad yet.
Summary of the invention
Technical problem to be solved by this invention provides a kind of polycaprolactone/polyethyleneglycol tissue engineering three-dimensional porous rack material and preparation method thereof, this timbering material possesses good hydrophilicity and controlled degradation rate and porosity, can be used for preparing tissue carrier material.
Polycaprolactone/polyethyleneglycol tissue engineering bracket material of the present invention, its component comprises: polycaprolactone (PCL) and Polyethylene Glycol (PEG), the proportioning of PCL and PEG is 100: 0～40: 60.
The molecular weight of described polycaprolactone is 1～1,000,000, and fusing point is 55～65 ℃.
The mean molecule quantity of described Polyethylene Glycol is 1000～20000, and fusing point is 50～54 ℃.
Described timbering material improves the hydrophilic of polymer surfaces by the solution blending of polycaprolactone and Polyethylene Glycol, can regulate the degradation rate of timbering material by the two proportioning of control PCL/PEG.
Of the present invention a kind of be the preparation of the tissue engineering bracket material of raw material with the polycaprolactone/polyethyleneglycol, comprising: (detailed process condition?)
Be (1) that 100: 0～40: 60 polycaprolactone and Polyethylene Glycol is dissolved in cosolvent (addition with proportioning?) in, stir to such an extent that concentration is 1～13% clear solution;
(2) adjusting electrostatic spinning voltage is 4～50kv, when the dynamic analysis of spinning fltting speed is 0.3～6ml/h, above-mentioned blend solution is spun into nanometer/sub-micron grade fiber felt;
(3) place vacuum drying oven to dry fiber felt, evacuation is removed residual solvent;
(4) take out fiber felt after, it is stacked and be filled in the mould with given shape;
(5) mould places vacuum drying oven, and near constant temperature 5～30min 50～56 ℃ takes out cooling and demolding, and the ultraviolet sterilization gets final product.
The described cosolvent of step (1) is oxolane or chloroform solvent, perhaps for adding the mixed solvent of one or more gained in dimethyl formamide, methanol, the ethanol.
The diameter of the fiber felt fiber in the step (2) is measured as 200～900nm through scanning electron microscope and Photoshop software.
The described temperature of step (5) is the blend melt temperature, and under this temperature, the fusing of the fiber of material surface is interconnected to form the three-D space structure that possesses certain intensity.
Beneficial effect
(1) adding of Polyethylene Glycol has improved the hydrophilic of polymer surfaces, has strengthened adhesion, the vegetative state of cell on the surface, by controlling the degradation speed that proportioning raw materials obtains controllable adjustment, satisfies the clinical needs of organizational project;
(2) fiber size that obtains of electrostatic spinning can be simulated the structure and the biological function of n cell epimatrix less than cell, and the desirable template of cell seeding, breeding, growth is provided;
(3) this fiber felt homogeneity is good, porosity is high, the aperture is adjustable, good biocompatibility, has definite shape and mechanical performance preferably with its three-dimensional porous structure material that makes.
Description of drawings
Fig. 1 is the PCL/PEG ear shape support outward appearance photo of electrostatic spinning technique and the preparation of fiber sintering method;
Fig. 2 is the stereoscan photograph of electrospinning PCL/PEG fiber felt.
The specific embodiment
Below in conjunction with specific embodiment, further set forth the present invention.Should be understood that these embodiment only to be used to the present invention is described and be not used in and limit the scope of the invention.Should be understood that in addition those skilled in the art can make various changes or modifications the present invention after the content of having read the present invention's instruction, these equivalent form of values fall within the application's appended claims institute restricted portion equally.
Adopt the tetrahydrochysene furan to feed as cosolvent, with molecular weight is 50,000, fusing point is 58～60 ℃ high polymer PCL, and mean molecule quantity is 1000, fusing point is that 50～54 ℃ macromolecule PEG is dissolved in the cosolvent with 80: 20 proportioning, stir, make transparent uniform solution after, electrostatic spinning is made the electrospinning fibre felt, place vacuum drying oven to dry, after removing residual solvent, it is stacked and be filled in the given shape mould to take out the back, and the vacuum drying oven temperature is set at 56 ℃, mould takes out after placing vacuum drying oven constant temperature 20min, the cooling back demoulding, ultraviolet sterilization, the tissue engineering bracket material that just obtains having given configuration and superperformance.Recording the fiber felt fibre diameter through scanning electron microscope and Photoshop software is 800 ± 100nm, and contact angle test display material surface hydrophilicity improves, and proves that tentatively degradation cycle shortens.This material possesses good degradation cycle, more is applicable to cartilage tissue engineered rack material.The contact angle test can simply be described.
Embodiment 2
Adopt oxolane and alcohol mixed solvent as cosolvent, with molecular weight is 100,000, fusing point is 58～60 ℃ high polymer PCL, and mean molecule quantity is 2000, fusing point is that 50～54 ℃ macromolecule PEG is dissolved in the cosolvent with 70: 30 proportioning, stir, after making clear solution, electrostatic spinning is made the electrospinning fibre felt, places vacuum drying oven to dry, and it is stacked and be filled in the given shape mould to take out the back, the vacuum drying oven temperature is set at 54 ℃, mould takes out after placing vacuum drying oven constant temperature 15min, the cooling back demoulding, the tissue engineering bracket material that just obtains having given configuration and superperformance.Recording the fiber felt fibre diameter through scanning electron microscope and Photoshop software is 300 ± 100nm, and contact angle test display material surface hydrophilicity improves, and proves that tentatively degradation cycle shortens.This material possesses good degradation cycle, more is applicable to cartilage tissue engineered rack material.
Embodiment 3
Adopt chloroform and methanol mixed solvent as cosolvent, with molecular weight is 1,000,000, fusing point is 58～60 ℃ high polymer PCL, and molecular weight is 2000, fusing point is that 50～54 ℃ macromolecule PEG is dissolved in the cosolvent with 50: 50 proportioning, stir, after making clear solution, electrostatic spinning is made the electrospinning fibre felt, places vacuum drying oven to dry, after removing residual solvent, it is stacked and be filled in the given shape mould to take out the back, and the vacuum drying oven temperature is set at 50 ℃, and mould takes out after placing vacuum drying oven constant temperature 5min, the cooling back demoulding, the tissue engineering bracket material that just obtains having given configuration and superperformance.Recording the fiber felt fibre diameter through scanning electron microscope and Photoshop software is 600 ± 100nm, and contact angle test display material surface hydrophilicity improves, and proves that tentatively degradation cycle shortens.This material possesses good degradation cycle, more is applicable to cartilage tissue engineered rack material.
Claims (6)
1. polycaprolactone/polyethyleneglycol tissue engineering bracket material, its component comprises: polycaprolactone and Polyethylene Glycol, the proportioning of polycaprolactone and Polyethylene Glycol are 100: 0～40: 60.
2. polycaprolactone/polyethyleneglycol tissue engineering bracket material according to claim 1, it is characterized in that: the molecular weight of described polycaprolactone is 1～1,000,000, fusing point is 55～65 ℃, and the mean molecule quantity of Polyethylene Glycol is 1000～20000, and fusing point is 50～54 ℃.
3. one kind is the preparation of the tissue engineering bracket material of raw material with the polycaprolactone/polyethyleneglycol, comprising:
(1) is that 100: 0～40: 60 polycaprolactone and Polyethylene Glycol is dissolved in the cosolvent with proportioning, stirs to such an extent that concentration is 1～13% clear solution;
(2) adjusting electrostatic spinning voltage is 4～50kv, when the dynamic analysis of spinning fltting speed is 0.3～6ml/h, above-mentioned blend solution is spun into nanometer/sub-micron grade fiber felt;
(3) place vacuum drying oven to dry fiber felt, evacuation is removed residual solvent;
(4) take out fiber felt after, it is stacked and be filled in the mould with given shape;
(5) mould places vacuum drying oven, in 50～56 ℃ of constant temperature 5～30min, takes out cooling and demolding, the ultraviolet sterilization.
4. according to claim 3 is the preparation of the tissue engineering bracket material of raw material with the polycaprolactone/polyethyleneglycol, it is characterized in that: the described cosolvent of step (1) is oxolane, chloroform solvent, or adds the mixed solvent of one or more gained in dimethyl formamide, methanol, the ethanol.
5. according to claim 3 is the preparation of the tissue engineering bracket material of raw material with the polycaprolactone/polyethyleneglycol, it is characterized in that: the diameter of the fiber felt fiber in the step (2) is measured as 200～900nm through scanning electron microscope and Photoshop software.
6. according to claim 3 is the preparation of the tissue engineering bracket material of raw material with the polycaprolactone/polyethyleneglycol, it is characterized in that: described 50～56 ℃ of temperature of step (5) are the blend melt temperature, under this temperature, the fiber fusing of material surface is interconnected to form the three-D space structure with intensity.
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Cited By (11)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN102068716A (en) * | 2010-12-29 | 2011-05-25 | 中国科学院长春应用化学研究所 | Method for preparing tissue engineering frame |
| CN103147165A (en) * | 2013-01-25 | 2013-06-12 | 四川大学 | Double-wall structured hollow ultrafine polymer fiber and preparation method thereof |
| CN107753996A (en) * | 2017-10-19 | 2018-03-06 | 五邑大学 | A kind of moisture absorption antibacterial 3D nano-fiber medical dressings and preparation method thereof |
| CN108355176A (en) * | 2018-02-12 | 2018-08-03 | 华中科技大学鄂州工业技术研究院 | A kind of composite nano fiber scaffold and its application |
| CN110665069A (en) * | 2019-10-14 | 2020-01-10 | 广东工业大学 | Preparation method of melt electrospinning composite fiber scaffold for bone repair and infection resistance |
| CN112057672A (en) * | 2020-07-14 | 2020-12-11 | 广东工业大学 | PCL-lignin nanofiber scaffold material and preparation method thereof |
| CN112552661A (en) * | 2020-12-10 | 2021-03-26 | 广州科莱瑞迪医疗器材股份有限公司 | Plastic tissue compensation glue and preparation method thereof |
| CN113262329A (en) * | 2021-04-21 | 2021-08-17 | 成都理工大学 | Hydroxyapatite/polycaprolactone/polyethylene glycol composite bone scaffold material |
| CN113289069A (en) * | 2021-04-28 | 2021-08-24 | 成都理工大学 | Polyurethane composite porous bone scaffold with high biological activity and preparation method thereof |
| CN113476653A (en) * | 2021-07-29 | 2021-10-08 | 石家庄学院 | Method for preparing porous tissue engineering scaffold constructed by fibers by in-situ fiber forming-particle leaching method |
| CN115105647A (en) * | 2022-06-17 | 2022-09-27 | 北京诺康达医药科技股份有限公司 | Beauty treatment pulling line and preparation method thereof |
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2008
- 2008-04-11 CN CNA2008100359521A patent/CN101288777A/en active Pending
Cited By (14)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN102068716B (en) * | 2010-12-29 | 2014-01-08 | 中国科学院长春应用化学研究所 | Method for preparing tissue engineering frame |
| CN102068716A (en) * | 2010-12-29 | 2011-05-25 | 中国科学院长春应用化学研究所 | Method for preparing tissue engineering frame |
| CN103147165A (en) * | 2013-01-25 | 2013-06-12 | 四川大学 | Double-wall structured hollow ultrafine polymer fiber and preparation method thereof |
| CN107753996A (en) * | 2017-10-19 | 2018-03-06 | 五邑大学 | A kind of moisture absorption antibacterial 3D nano-fiber medical dressings and preparation method thereof |
| CN108355176A (en) * | 2018-02-12 | 2018-08-03 | 华中科技大学鄂州工业技术研究院 | A kind of composite nano fiber scaffold and its application |
| CN110665069B (en) * | 2019-10-14 | 2021-11-26 | 广东工业大学 | Preparation method of melt electrospinning composite fiber scaffold for bone repair and infection resistance |
| CN110665069A (en) * | 2019-10-14 | 2020-01-10 | 广东工业大学 | Preparation method of melt electrospinning composite fiber scaffold for bone repair and infection resistance |
| CN112057672A (en) * | 2020-07-14 | 2020-12-11 | 广东工业大学 | PCL-lignin nanofiber scaffold material and preparation method thereof |
| CN112552661A (en) * | 2020-12-10 | 2021-03-26 | 广州科莱瑞迪医疗器材股份有限公司 | Plastic tissue compensation glue and preparation method thereof |
| CN113262329A (en) * | 2021-04-21 | 2021-08-17 | 成都理工大学 | Hydroxyapatite/polycaprolactone/polyethylene glycol composite bone scaffold material |
| CN113289069A (en) * | 2021-04-28 | 2021-08-24 | 成都理工大学 | Polyurethane composite porous bone scaffold with high biological activity and preparation method thereof |
| CN113476653A (en) * | 2021-07-29 | 2021-10-08 | 石家庄学院 | Method for preparing porous tissue engineering scaffold constructed by fibers by in-situ fiber forming-particle leaching method |
| CN115105647A (en) * | 2022-06-17 | 2022-09-27 | 北京诺康达医药科技股份有限公司 | Beauty treatment pulling line and preparation method thereof |
| WO2023241357A1 (en) * | 2022-06-17 | 2023-12-21 | 北京诺康达医药科技股份有限公司 | Cosmetic lifting thread and preparation method therefor |
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Open date: 20081022 |