CN110692624A - Extraction device for cell cryopreservation - Google Patents
Extraction device for cell cryopreservation Download PDFInfo
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- CN110692624A CN110692624A CN201910962033.7A CN201910962033A CN110692624A CN 110692624 A CN110692624 A CN 110692624A CN 201910962033 A CN201910962033 A CN 201910962033A CN 110692624 A CN110692624 A CN 110692624A
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- rod
- guide wheel
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- lifting rod
- protrusion
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- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01N—PRESERVATION OF BODIES OF HUMANS OR ANIMALS OR PLANTS OR PARTS THEREOF; BIOCIDES, e.g. AS DISINFECTANTS, AS PESTICIDES OR AS HERBICIDES; PEST REPELLANTS OR ATTRACTANTS; PLANT GROWTH REGULATORS
- A01N1/00—Preservation of bodies of humans or animals, or parts thereof
- A01N1/02—Preservation of living parts
- A01N1/0236—Mechanical aspects
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Abstract
The extraction device for cell cryopreservation is used for quickly and accurately taking out a required cell sample from a liquid nitrogen tank. It includes: the freezing rack is provided with a hanging hole at the upper part, four rows of small lattices which are sequentially arranged from left to right are arranged in the front end surface of the freezing rack, a plurality of small lattices arranged in rows are arranged at equal intervals from top to bottom, a box is placed in the small lattices, and jacks are arranged in the front end surface of the box; the rear side of the lifting rod is provided with a protrusion, the front end face of the cryopreservation frame is provided with a sliding groove, the bottom of the sliding groove is provided with a protrusion groove corresponding to the protrusion, the left side and the right side of the lower part of the lifting rod are respectively provided with a side rod, a supporting rod is fixed on the side rods, and the supporting rods are in inserted fit with the insertion holes; the unloading unit is arranged in the supporting rod, the supporting rod and the jacks are relatively fixed and separated under the action of the unloading unit, and the lifting rod is provided with scale marks corresponding to each row of small grids. The device can be convenient and accurate to realize the storage and extraction of the cryopreservation tube.
Description
Technical Field
The invention relates to the technical field of biological freezing, in particular to an extraction device for cell cryopreservation.
Background
The cell is usually frozen and is preserved in the liquid nitrogen jar, and the liquid nitrogen temperature in the liquid nitrogen jar is at-196 degrees centigrade, when extracting the frozen cell in the liquid nitrogen jar, the bare-handed person can not direct contact cryopreserved pipe or save the frame (cryopreserved pipe is placed on preserving the frame), and because the reason of sight, also can't find the cell of required extraction fast. Therefore, there is a need for a cell cryopreservation extraction device for rapidly and accurately extracting the desired cells from a liquid nitrogen tank.
Disclosure of Invention
The invention aims to provide an extraction device for cell cryopreservation, which is used for quickly and accurately taking out a required cell sample from a liquid nitrogen tank.
The technical scheme adopted by the invention for solving the technical problems is as follows: extraction element of cell cryopreservation, characterized by that, it includes:
the freezing frame is of a rectangular structure, a hanging hole is formed in the upper portion of the freezing frame, four rows of small grids which are sequentially arranged from left to right are arranged in the front end face of the freezing frame, a plurality of small grids which are arranged in a row are arranged at equal intervals from top to bottom, a box is placed in each small grid, a freezing pipe is arranged in each box, and a jack is formed in the front end face of each box;
the rear side of the lower part of the lifting rod is provided with a protrusion, the front end face of the cryopreservation frame is provided with a sliding groove, the bottom of the sliding groove is provided with a protrusion groove corresponding to the protrusion, the number of the protrusion grooves is the same as that of the small lattices, the small lattices correspond to the small lattices in the rows one to one, the left side and the right side of the lower part of the lifting rod are respectively provided with a side rod, a supporting rod is fixed on the side rod, and the supporting rod is in inserting fit with the insertion hole; the unloading unit is arranged on the lifting rod, the support rod and the jacks are relatively fixed and separated under the action of the unloading unit, and the lifting rod is provided with scale marks corresponding to each row of small grids.
Furthermore, a hollow handle is fixed on the top of the lifting rod, and the driving mechanism is arranged between the handle and the side rod.
Furthermore, the unloading unit comprises a pair of elastic pieces fixed in the first end of the supporting rod, a positioning block fixed on the elastic pieces and a driving mechanism for driving the elastic pieces to expand outwards, wherein a notch is formed in the first end of the supporting rod, and the positioning block can be driven to extend out of the notch under the action of the driving mechanism.
Furthermore, the driving mechanism comprises a first screw rod and a second screw rod which are in threaded connection with the handle, a first reel which is fixed on the first screw rod, a second reel which is fixed on the second screw rod, a first guide wheel and a second guide wheel which are rotatably installed in the inner cavity of the handle, a third guide wheel and a fourth guide wheel which are rotatably installed in the inner cavity of the lower part of the lifting rod, a fifth guide wheel and a sixth guide wheel which are rotatably installed in the side rods, a first pull wire which is arranged between the first reel and the corresponding driving block, and a second pull wire which is arranged between the second reel and the corresponding driving block, wherein the first pull wire is in contact with the outer walls of the first guide wheel, the third guide wheel and the fifth guide wheel, and the second pull wire is in contact with the outer walls of the second guide wheel, the fourth guide wheel and the sixth guide wheel, a driving block is movably arranged in the supporting rod, a baffle is also fixed in the supporting rod, and a spring is arranged between the baffle and the driving block.
Furthermore, a first knob arranged outside the handle is fixed on the first screw rod, and a second knob arranged outside the handle is fixed on the second screw rod.
Furthermore, four nuts are fixed in the inner cavity of the handle, and the first screw and the second screw are in threaded connection with the nuts.
Furthermore, a pair of square grooves arranged on the left and right sides are formed in the freezing storage frame, vertical plates are fixed in the square grooves, a plurality of transverse plates are arranged between the vertical plates and the inner walls of the square grooves, and small lattices are formed among the transverse plates, the vertical plates and the inner walls of the square grooves.
The invention has the beneficial effects that: the extraction device for cell cryopreservation can arrange the cryopreservation tubes in order, and only the cryopreservation frame needs to be hung on the hook on the inner wall of the liquid nitrogen tank when the cryopreservation frame is placed. When depositing and freezing the deposit pipe, need not take out whole freezing the deposit pipe, only need will be equipped with the box that freezes the deposit pipe and place in the cell of corresponding position through carrying the pull rod. When drawing the cryopreserving pipe, also only need to carry the pull rod to install on cryopreserving frame, carry the position of pull rod through sliding, can arrange the bracing piece in required vertical position, can control the bracing piece action that corresponds the transverse position through first and second knob in order to grasp the box that corresponds, and then take out the box that corresponds in the swing through carrying the pull rod. The whole device, no matter deposit or draw of freezing the pipe, all need not take out whole freezing frame.
Drawings
FIG. 1 is a schematic front view of the present invention;
FIG. 2 is a front view of the cryopreservation rack;
FIG. 3 is a front view of the lifting bar;
FIG. 4 is a side view of the lifting bar;
FIG. 5 is one of the schematic views of the drive mechanism;
FIG. 6 is a second schematic view of the driving mechanism;
FIG. 7 is one of the cross-sectional views of the support rod;
FIG. 8 is a second cross-sectional view of the support rod;
FIG. 9 is a front view of the cassette;
FIG. 10 is an enlarged view of a portion of FIG. 4 at A;
FIG. 11 is a view showing the positional relationship among the slide grooves, the projecting grooves and the long grooves;
in the figure: 1, 11 square grooves, 12 vertical plates, 13 transverse plates, 14 small grids, 15 boxes, 151 inserting holes, 152 positioning holes, 16 sliding grooves, 161 long grooves, 162 protruding grooves, 17 hanging holes, 2 lifting rods, 21 protrusions, 211 short shafts, 22 side rods, 23 handles, 231 nuts, 24 first knobs, 241 first screw rods, 242 first winding wheels, 25 second knobs, 251 second screw rods, 252 second winding wheels, 26 first guide wheels, 27 second guide wheels, 28 first pull wires, 29 second pull wires, 3 third guide wheels, 31 fourth guide wheels, 32 fifth guide wheels, 33 sixth guide wheels, 4 supporting rods, 41 gaps, 42 fixing plates, 43 driving blocks, 44 springs, 45 elastic plates and 46 positioning blocks.
Detailed Description
As shown in fig. 1 to 11, the present invention mainly includes a freezing rack 1 and a lifting rod 2, and the present invention will be described in detail with reference to the accompanying drawings.
As shown in fig. 1 and 2, the freezing storage frame 1 is of a rectangular structure, can be plastic parts, is provided with a pair of square grooves 11 which are arranged left and right on the freezing storage frame, is provided with a vertical plate 1 in the square groove, is provided with a plurality of transverse plates 13 between the inner walls of the vertical plate and the square grooves, forms a plurality of small lattices 14 between the transverse plates, the vertical plate and the inner walls of the square grooves, places a box 15 in the small lattices, and is provided with a jack 151 in the front surface of the box, and is provided with a pair of positioning holes 152 on the inner wall of the jack. When the freezing storage box is used, the freezing storage tubes are sequentially placed into the box, and each box can contain 5-10 freezing storage tubes. A sliding groove 16 is arranged in the front surface of the freezing frame between the two long grooves, a pair of long grooves 161 are arranged on the inner wall of the sliding groove, and a hanging hole 17 is arranged on the upper part of the freezing frame and used for hanging the freezing frame on a hook on the inner wall of the liquid nitrogen tank. As shown in fig. 2 and 11, the bottom of the sliding groove is provided with protruding grooves 162 corresponding to the cells arranged in rows, and the freezing rack can be hooked by the protrusions after the protrusions extend into the protruding grooves.
As shown in fig. 3 and 4, the lifting bar 2 is a straight bar, a protrusion 21 is fixed to a lower portion of a rear side wall of the lifting bar, and short shafts 211 are provided on left and right side walls of the protrusion, respectively, as shown in fig. 10. A side rod 22 is fixed on the left and right side walls of the lower part of the lifting rod, respectively, and a handle 23 is fixed on the top of the lifting rod, as shown in fig. 5, the handle is a hollow structure, and four nuts 231 are fixed in the inner cavity of the handle. Two first knobs 24 arranged left and right and two second knobs 25 arranged left and right are arranged above the handle, and the second knob is positioned between the two first knobs. The two first knobs and the two second knobs are respectively positioned on four vertexes of the isosceles trapezoid. A first screw 241 is fixed to the bottom of the first knob, a first reel 242 is fixed to the first screw, a first cord 28 is wound around the first reel, and a first end of the first cord is tied to an outer wall of the first reel. A second screw 251 is fixed to the bottom of the second knob, a second reel 252 is fixed to the second screw, a second wire 29 is wound around the second reel, and a first end of the second wire is tied to the outer wall of the second reel. Between the two second reels are two first guide wheels 26, which are arranged to the left and right, and two second guide wheels 27, which are arranged to the left and right, and above which the first guide wheels are located. The first pull wire is in contact with the outer wall of the first guide wheel, and the second pull wire is in contact with the outer wall of the second guide wheel. After the first pull wire is guided by the first guide wheel and the second pull wire is guided by the second guide wheel, the second ends of the first pull wire and the second pull wire extend into the lifting rod. As shown in fig. 6, two third guide wheels 3 arranged left and right and two fourth guide wheels 31 arranged left and right are arranged at the lower part of the inner cavity of the lifting rod, and the fourth guide wheels are positioned in the space between the two third guide wheels. The two third guide wheels and the two fourth guide wheels are respectively positioned on four vertexes of the isosceles trapezoid, and the third guide wheels and the fourth guide wheels are positioned in the same horizontal plane. The first pull wire is in contact with the outer wall of the third guide wheel, and the second pull wire is in contact with the outer wall of the fourth guide wheel. And a fifth guide wheel 32 and a sixth guide wheel 33 are further arranged in the plane where the third guide wheel and the fourth guide wheel are located, the first pull wire is in contact with the outer wall of the fifth guide wheel, and the second pull wire is in contact with the outer wall of the sixth guide wheel. The third guide wheel and the fourth guide wheel are rotatably arranged at the lower part of the lifting rod, and the fifth guide wheel and the sixth guide wheel are rotatably arranged in the side rod. Two support rods 4 are fixed on the rear side wall of each side rod, as shown in fig. 7 and 8, each support rod is of a hollow structure, the first end of each support rod is hemispherical, a notch 41 is arranged at the first end of each support rod and penetrates through the side wall of the first end of each support rod, a baffle 42 is further fixed in the inner cavity of the first end of each support rod, a central hole is formed in the center of each baffle, a driving block 43 is movably arranged in the inner cavity of the first end of each support rod, a spring 44 is arranged between each driving block and each baffle, and the driving blocks are in contact with the circular inner wall of the first end of each support rod under. As shown in fig. 8, an elastic piece 45 is fixed on the inner wall of the first end of the first support rod, the first end of the elastic piece is fixedly connected with the inner wall of the support rod, a positioning block 46 is arranged at the second end of the elastic piece, and the positioning block is arranged in the notch under the natural state. The elastic sheet and the positioning block are arranged between the driving block and the baffle, the number of the supporting rods is four, the second ends of the two first pull wires extend into the two supporting rods and are tied together with the driving block, and the second ends of the two second pull wires extend into the other two supporting rods and are tied together with the driving block. When rotatory first knob, can order about first acting as go-between winding on first reel, and then stimulate the drive block in two of them bracing pieces and remove to the one side that is close to the baffle, until the second end contact of drive block and flexure strip, the drive block extrusion flexure strip makes the locating piece stretch out the breach this moment, after the locating piece stretched into the locating hole, can realize the relative fixation of box and bracing piece, can pull out the box from the cell like this, or put into the cell with the box. The first knob, the second knob, the first reel, the second reel, the first guide wheel, the second guide wheel, the third guide wheel, the fourth guide wheel, the fifth guide wheel, the sixth guide wheel, the first stay wire, the second stay wire, the elastic sheet, the positioning block, the driving block, the spring and the baffle plate form an unloading unit, wherein the first knob, the second knob, the first reel, the second reel, the first guide wheel, the second guide wheel, the third guide wheel, the fourth guide wheel, the fifth guide wheel, the sixth guide wheel, the first stay wire, the second stay wire, the driving block, the spring and the baffle plate form a driving mechanism, the driving mechanism is used for driving the positioning block on the elastic sheet to expand outwards, and the whole unloading unit is used for realizing the relative fixation and separation between the supporting rod and the jack.
The method of use of the invention is described below:
(1) placing the cryopreservation tube in a box, then extending a support rod corresponding to the storage position into the jack according to the storage position, and then rotating a first knob or a second knob corresponding to the support rod to enable the positioning block to extend out of the notch and then to be placed in the positioning hole, wherein the support rod and the box are relatively fixed;
(2) the protrusion at the lower part of the lifting rod is arranged in the sliding groove, the protrusion is arranged in the protrusion groove at the corresponding position, the short shaft extends into the long groove, and the lifting rod is ensured to be in an inclined state at the moment;
(3) the freezing rack is hung on a hook on the inner wall of the liquid nitrogen tank by holding a lifting rod;
(4) swinging the lifting rod to one side where the freezing storage rack is located to enable the box on the supporting rod to extend into the small cell, reversely rotating the first knob or the second knob until the box is in contact with the rear wall of the small cell to enable the positioning block on the supporting rod to be moved out of the positioning block, and then reversely moving the lifting rod to enable the supporting rod to be far away from the freezing storage rack until the supporting rod is moved out of the jack, so that the freezing storage pipe is stored;
(5) holding the lifting rod, enabling the lifting rod to move to a required position, then enabling the lifting rod to swing to one side close to the box until the supporting rod stretches into the insertion hole, then rotating the first knob or the second knob of the corresponding row of the position to be taken, enabling the positioning block to stretch into the positioning hole in the box, then swinging the lifting rod reversely, enabling the box to move out of the small grid, and then upwards lifting the lifting rod, and enabling the lifting rod to move out of the liquid nitrogen tank.
When the protrusion is arranged at the lowest point of the sliding chute, the handle at the top of the lifting rod is arranged outside the liquid nitrogen tank, and the lifting rod is arranged outside the liquid nitrogen tank in a storage state; when the freezing storage pipe needs to be stored, the box with the freezing storage pipe is placed in the small grid through the lifting rod. When the protrusions are arranged in different protrusion grooves, the lengths of the lifting rods extending out of the liquid nitrogen tank are different, and the protrusions are judged to be arranged in the protrusion grooves of the rows. Therefore, the scale marks are arranged on the outer wall of the lifting rod, and when the protrusion is arranged in the protrusion groove at the lowest part, the scale marks at the highest part of the lifting rod are aligned with the opening of the liquid nitrogen tank; when the protrusion is arranged in the 5 th protrusion groove from bottom to top, the 5 th scale line of the lifting rod is counted from top to bottom and is aligned with the opening of the liquid nitrogen tank, and so on. Thus, the supporting rod is arranged at the required vertical position through the relation between the scale mark and the opening of the liquid nitrogen tank; through operating corresponding first or second knob for the bracing piece in horizontal position plays a role, realizes depositing, getting to the box.
Claims (7)
1. Extraction element of cell cryopreservation, characterized by that, it includes:
the freezing frame is of a rectangular structure, a hanging hole is formed in the upper portion of the freezing frame, four rows of small grids which are sequentially arranged from left to right are arranged in the front end face of the freezing frame, a plurality of small grids which are arranged in a row are arranged at equal intervals from top to bottom, a box is placed in each small grid, a freezing pipe is arranged in each box, and a jack is formed in the front end face of each box;
the rear side of the lower part of the lifting rod is provided with a protrusion, the front end face of the cryopreservation frame is provided with a sliding groove, the bottom of the sliding groove is provided with a protrusion groove corresponding to the protrusion, the number of the protrusion grooves is the same as that of the small lattices, the small lattices correspond to the small lattices in the rows one to one, the left side and the right side of the lower part of the lifting rod are respectively provided with a side rod, a supporting rod is fixed on the side rod, and the supporting rod is in inserting fit with the insertion hole; the unloading unit is arranged on the lifting rod, the support rod and the jacks are relatively fixed and separated under the action of the unloading unit, and the lifting rod is provided with scale marks corresponding to each row of small grids.
2. The extraction apparatus for cell cryopreservation as claimed in claim 1, wherein a hollow handle is fixed to the top of the lifting rod, and the driving mechanism is provided between the handle and the side rod.
3. The extraction apparatus for cell cryopreservation as claimed in claim 1, wherein the unloading unit comprises a pair of elastic pieces fixed in the first end of the support rod, a positioning block fixed on the elastic pieces, and a driving mechanism for driving the elastic pieces to expand, wherein the first end of the support rod is provided with a notch, and the positioning block can be driven to extend out of the notch by the driving mechanism.
4. The device for extracting frozen cells according to claim 3, wherein the driving mechanism comprises a first screw rod and a second screw rod which are connected with the handle by screw threads, a first reel fixed on the first screw rod, a second reel fixed on the second screw rod, a first guide wheel and a second guide wheel which are rotatably installed in the inner cavity of the handle, a third guide wheel and a fourth guide wheel which are rotatably installed in the inner cavity of the lower part of the lifting rod, a fifth guide wheel and a sixth guide wheel which are rotatably installed in the side rod, a first pull wire arranged between the first reel and the corresponding driving block, a second pull wire arranged between the second reel and the corresponding driving block, the first pull wire is in contact with the outer walls of the first guide wheel, the third guide wheel and the fifth guide wheel, the second pull wire is in contact with the outer walls of the second guide wheel, the fourth guide wheel and the sixth guide wheel, and a driving block is movably arranged in the supporting rod, a baffle is also fixed in the support rod, and a spring is arranged between the baffle and the driving block.
5. The extraction device for cell cryopreservation according to claim 4, wherein a first knob is fixed to the first screw and a second knob is fixed to the second screw.
6. The extraction device for cell cryopreservation as claimed in claim 5, wherein four nuts are fixed in the inner cavity of the handle, and the first and second screws are both in threaded connection with the nuts.
7. The extraction apparatus for cell cryopreservation according to claim 1, wherein a pair of square grooves are provided on the cryopreservation rack, a vertical plate is fixed in each square groove, a plurality of horizontal plates are provided between the vertical plate and the inner wall of the square groove, and cells are formed among the horizontal plates, the vertical plates, and the inner wall of the square groove.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201910962033.7A CN110692624B (en) | 2019-10-11 | 2019-10-11 | Extraction device for cell cryopreservation |
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| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201910962033.7A CN110692624B (en) | 2019-10-11 | 2019-10-11 | Extraction device for cell cryopreservation |
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| Publication Number | Publication Date |
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| CN110692624A true CN110692624A (en) | 2020-01-17 |
| CN110692624B CN110692624B (en) | 2021-09-28 |
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| CN201910962033.7A Active CN110692624B (en) | 2019-10-11 | 2019-10-11 | Extraction device for cell cryopreservation |
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Cited By (2)
| Publication number | Priority date | Publication date | Assignee | Title |
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| CN113016785A (en) * | 2021-03-23 | 2021-06-25 | 浙江大学 | Embryo freezing technology is with save set who selects freezing embryo accurately |
| CN115553287A (en) * | 2022-10-27 | 2023-01-03 | 北京标驰泽惠生物科技有限公司 | Tick preserving fluid and preparation method and application thereof |
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| Publication number | Priority date | Publication date | Assignee | Title |
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| CN113016785A (en) * | 2021-03-23 | 2021-06-25 | 浙江大学 | Embryo freezing technology is with save set who selects freezing embryo accurately |
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| CN115553287B (en) * | 2022-10-27 | 2023-08-08 | 北京标驰泽惠生物科技有限公司 | Tick preservation solution and preparation method and application thereof |
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| CN110692624B (en) | 2021-09-28 |
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