CN110686961A - But reuse's temperature regulation type paraffin embedding box - Google Patents
But reuse's temperature regulation type paraffin embedding box Download PDFInfo
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- CN110686961A CN110686961A CN201911046469.8A CN201911046469A CN110686961A CN 110686961 A CN110686961 A CN 110686961A CN 201911046469 A CN201911046469 A CN 201911046469A CN 110686961 A CN110686961 A CN 110686961A
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- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N1/00—Sampling; Preparing specimens for investigation
- G01N1/28—Preparing specimens for investigation including physical details of (bio-)chemical methods covered elsewhere, e.g. G01N33/50, C12Q
- G01N1/36—Embedding or analogous mounting of samples
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- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N1/00—Sampling; Preparing specimens for investigation
- G01N1/02—Devices for withdrawing samples
- G01N1/04—Devices for withdrawing samples in the solid state, e.g. by cutting
- G01N1/06—Devices for withdrawing samples in the solid state, e.g. by cutting providing a thin slice, e.g. microtome
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- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N1/00—Sampling; Preparing specimens for investigation
- G01N1/28—Preparing specimens for investigation including physical details of (bio-)chemical methods covered elsewhere, e.g. G01N33/50, C12Q
- G01N1/42—Low-temperature sample treatment, e.g. cryofixation
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- Life Sciences & Earth Sciences (AREA)
- Chemical & Material Sciences (AREA)
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- General Health & Medical Sciences (AREA)
- General Physics & Mathematics (AREA)
- Immunology (AREA)
- Pathology (AREA)
- Sampling And Sample Adjustment (AREA)
Abstract
The invention discloses a recyclable temperature-adjustable paraffin embedding box, which comprises a box body, an embedding seat, a wax bearing plate, a bearing platform and a temperature adjusting device, wherein a cavity is arranged in the box body, a sealing cover is clamped at the upper end of the box body, the embedding seat is clamped in the box body, an embedding groove is formed in the embedding seat, the wax bearing plate is clamped at the upper end in the box body, a through groove is formed in the wax bearing plate, a porous plate is arranged in the through groove, the bearing platform is clamped at the lower end of the through groove, the temperature adjusting device comprises a water cooler, a heat-insulating pipe and a temperature adjuster, the water cooler is arranged at the lower end of the cavity, the water cooler is powered by an external power supply, the heat-insulating pipe is wound on an inner breathable plate, the heat-insulating pipe is connected with the water cooler, the temperature; the paraffin block solidifying and demolding device is reasonable in structural design, can realize rapid solidification and demolding of paraffin blocks, and is suitable for large-scale popularization.
Description
Technical Field
The invention relates to the technical field of embedding boxes, in particular to a reusable temperature-adjusting paraffin embedding box.
Background
Embedding, also called tissue embedding or paraffin embedding, is the process of embedding tissue wax blocks after dehydrating and waxing a human body or an animal and plant specimen for histological diagnosis or research after slicing, and is a process of wrapping material powder or other block structures to be embedded by using embedding agents such as organic plastics, metals, paraffin and the like to provide performance support or chemical protection. In the medical field, it is often necessary to analyze the morphology of a biological tissue sample to obtain relevant physiological or pathological information to facilitate accurate judgment by a researcher. The paraffin embedding section method is because can obtain comparatively clear tissue morphology structure, so obtain more and more extensive application, arrange the embedding box in during the embedding on the hot platform, pour into partial liquid paraffin in to the embedding box, put into the tissue sample, treat that embedding box bottom paraffin begins when solidifying, gently press the tissue sample, will dewater box embedding box upper portion, pour into paraffin again in to the embedding box, make the paraffin liquid level be higher than the dehydration box bottom, move the embedding box to the cold platform and treat that the temperature drops, paraffin solidifies completely, take out dehydration box and the paraffin embedding piece that links to each other, accomplish the embedding process.
However, the existing paraffin embedding box does not have a temperature regulation function, and the paraffin solution can be rapidly cooled and solidified only by an external device, so that the embedding progress is influenced, time and labor are wasted, and the paraffin block is easy to damage in the demolding process, so that the accuracy of slice observation is influenced; on the other hand, most of the existing embedding boxes are disposable and cannot be reused, so that the resource is greatly wasted.
Disclosure of Invention
Aiming at the technical problems, the invention provides a reusable temperature-adjusting paraffin embedding box capable of quickly demoulding.
The technical scheme of the invention is as follows: a temperature regulation type paraffin embedding box capable of being reused comprises a box body, an embedding seat, a paraffin bearing plate, a bearing platform and a temperature regulation device; the box body comprises an outer insulation board and an inner ventilation board, a cavity is formed between the outer insulation board and the inner ventilation board, a sealing cover is clamped at the upper end of the box body, and a handle is arranged on the sealing cover; the embedding seat is movably clamped inside the box body and clamped with the box body through a bolt, embedding grooves are arranged on the embedding seat in a matrix mode, the upper ends of the embedding grooves are of a cubic structure, and the lower ends of the embedding grooves are of a chamfered platform structure; the wax bearing plate is movably clamped at the upper end in the box body and is positioned at the upper end of the embedding seat, through grooves are formed in the wax bearing plate, the positions and the number of the through grooves correspond to those of the embedding grooves, porous plates are horizontally arranged in the through grooves, and lifting plates are arranged at two ends of the upper surface of the wax bearing plate; the bearing platform is movably clamped at the lower end of the through groove, the interior of the bearing platform is hollow, and an opening at the lower end of the bearing platform is embedded with an opening at the upper end of the embedding groove; the temperature adjusting device comprises a water cooler, a heat preservation pipe and a temperature adjuster, the water cooler is arranged at the lower end of the cavity, the water cooler is powered by an external power supply, the heat preservation pipe is wound on the inner ventilating plate and is connected with the water cooler, the temperature adjuster is fixedly arranged on the outer wall of the box body, and the temperature adjuster is connected with the water cooler.
Further, still include the vibration subassembly, the vibration subassembly is including shaking board and vibrating motor a little, the board activity joint that shakes a little is at the inside lower extreme of embedding seat, vibrating motor is fixed to be set up on the outer wall of box body, vibrating motor is connected with the board that shakes a little, vibrating motor provides power for the board that shakes a little, vibrating motor is supplied power by external power source, through setting up board and vibrating motor a little, make the paraffin piece after solidifying compacter, place paraffin solidification in-process and appear hollowing phenomenon, influence the section and observe.
Furthermore, the perforated plate is of an upward-convex arc-shaped structure, and the aperture of the through hole at the central position of the perforated plate is larger than that of the through hole at the edge position of the perforated plate; the paraffin solution can be uniformly and smoothly dripped into the embedding groove.
Further, embedding groove upper end opening part is provided with the maintenance doctor-bar, and the maintenance doctor-bar slope sets up downwards, through setting up the decurrent maintenance doctor-bar of slope, is convenient for maintain the paraffin block corner after solidifying, avoids the artifical clearance of later stage secondary, has alleviateed researcher's working strength, has improved work efficiency.
Further, the coating has anti-adhesion coating on the embedding groove inner wall, and through setting up anti-adhesion coating, the paraffin piece after being convenient for solidify breaks away from the embedding groove, prevents that paraffin piece and embedding groove inner wall from bonding, suffers destruction after taking out under the exogenic action.
Furthermore, the movable pin is clamped at the upper end of the bearing platform, a reset spring is arranged at the joint of the movable pin and the bearing platform, a pull rod is arranged at the lower end of the movable pin, a through groove used for sliding the pull rod left and right is formed in the bearing platform, a clamping groove corresponding to the movable pin is formed in the through groove, the bearing platform and the wax bearing plate are clamped through the movable pin and the clamping groove, and the bearing platform and the solidified paraffin block can be conveniently taken out together.
Further, the level is provided with annular anticreep strip on the inner wall of cushion cap, and when taking out the paraffin piece, the wax board is held in the rebound, makes the paraffin piece break away from the embedding groove together with the cushion cap, and when being convenient for slice, fixes the paraffin piece.
The working principle of the invention is as follows: when the paraffin embedding tank is used, the water cooler and the vibration motor are respectively connected with an external power supply, then a small amount of paraffin solution is injected into the embedding tank, then the dehydrated tissue is placed in the paraffin solution and flattened by using medical forceps, the bearing platform is arranged at the lower part of the through groove on the paraffin bearing plate, the bearing platform and the paraffin bearing plate are fixed through the movable pin and the clamping groove, and the paraffin solution is injected into the embedding tank through the porous plate until the page is close to the porous plate and then stops; starting a vibration motor, carrying out vibration treatment on the paraffin solution in the embedding groove by using a micro-vibration plate, then starting a water cooling machine, and adjusting the temperature of a heat preservation pipe by using a temperature regulator to quickly solidify the paraffin solution; after the paraffin solution is completely solidified, adjusting the temperature of the heat preservation pipe to room temperature, covering a sealing cover to store the paraffin block, when the paraffin block needs to be subjected to tissue slicing, taking out the paraffin bearing plate, the bearing platform and the solidified paraffin block together by holding a lifting plate, and removing corner burrs of the solidified paraffin block under the action of a trimming scraper; remove the movable pin, follow paraffin block and cushion cap and take off on bearing the wax board, with the cushion cap centre gripping on the slicer carry out tissue slice to the paraffin block can.
Compared with the prior art, the invention has the beneficial effects that: the paraffin block embedding device is reasonable in structural design, and by arranging the embedding groove capable of adjusting the temperature, the paraffin solution can be quickly solidified, the embedding efficiency is improved, and meanwhile, the solidified paraffin block can be stored, so that the influence on the accuracy of observation of a tissue section caused by the fact that the paraffin block is polluted by the external environment is avoided; by arranging the bearing platform, demolding and slicing fixing of the paraffin block are facilitated, uniformity of tissue slices is provided, and observation of the tissue slices is facilitated; the scraping blade is arranged on the embedding groove, so that burrs at the corners of the paraffin blocks can be scraped conveniently, and the working efficiency is improved.
Drawings
FIG. 1 is a schematic structural view of the present invention;
FIG. 2 is a left side view of the present invention;
FIG. 3 is a schematic view of the connection between the embedding base and the box body according to the present invention;
FIG. 4 is a schematic structural view of the wax bearing plate of the present invention;
FIG. 5 is a schematic view of the connection between the embedding seat and the wax bearing plate according to the present invention;
FIG. 6 is a schematic view of the structure of the embedding base of the present invention;
the device comprises a box body 1, an outer heat insulation plate 10, an inner breathable plate 11, a cavity 12, a sealing cover 13, a handle 130, an embedding seat 2, an embedding groove 20, a trimming scraping sheet 200, a wax bearing plate 3, a through groove 30, a perforated plate 31, a pulling plate 32, a bearing platform 4, an annular anti-dropping strip 40, a temperature adjusting device 5, a water cooling machine 50, a heat insulation pipe 51, a temperature adjuster 52, a vibration assembly 6, a micro-vibration plate 60, a vibration motor 61, a movable pin 7, a reset spring 70 and a pull rod 71.
Detailed Description
Example (b): the reusable temperature-adjusting paraffin embedding box shown in fig. 1 comprises a box body 1, an embedding seat 2, a wax bearing plate 3, a bearing platform 4, a temperature adjusting device 5 and a vibration assembly 6; the box body 1 comprises an outer insulation board 10 and an inner ventilation board 11, a cavity 12 is formed between the outer insulation board 10 and the inner ventilation board 11, a sealing cover 13 is clamped at the upper end of the box body 1, and a handle 130 is arranged on the sealing cover 13;
as shown in fig. 1 and 3, the embedding seat 2 is movably clamped inside the box body 1 and clamped with the box body 1 through bolts, embedding grooves 20 are arranged on the embedding seat 2 in a 2 x 3 matrix form, the upper ends of the embedding grooves 20 are of a cubic structure, and the lower ends of the embedding grooves 20 are of a chamfered platform structure; a trimming scraper 200 is arranged at an opening at the upper end of the embedding groove 20, the trimming scraper 200 is arranged obliquely downwards, and the edge of the solidified paraffin block is trimmed conveniently by arranging the trimming scraper 200 which is inclined downwards, so that secondary manual cleaning at the later stage is avoided, the working strength of researchers is reduced, and the working efficiency is improved; coating has anti-adhesion coating on the embedding groove 20 inner wall, through setting up anti-adhesion coating, the paraffin piece after being convenient for solidify breaks away from embedding groove 20, prevents that paraffin piece and 20 inner walls of embedding groove from bonding, suffers destruction after taking out under the exogenic action, and anti-adhesion coating is the teflon coating.
As shown in fig. 1, 4 and 5, the wax bearing plate 3 is movably clamped at the upper end inside the box body 1 and is positioned at the upper end of the embedding seat 2, the wax bearing plate 3 is provided with through grooves 30, the positions and the number of the through grooves 30 correspond to those of the embedding grooves 20, porous plates 31 are horizontally arranged inside the through grooves 30, the porous plates 31 are of an upward-convex arc-shaped structure, and the aperture of a through hole at the center of each porous plate 31 is larger than that of a through hole at the edge of each porous plate 31; the paraffin solution can be uniformly and flatly dripped into the embedding groove 20; the two ends of the upper surface of the wax bearing plate 3 are provided with the lifting plates 32;
as shown in fig. 5 and 6, the bearing platform 4 is movably clamped at the lower end of the through groove 30, the bearing platform 4 is hollow, the lower end opening of the bearing platform 4 is embedded with the upper end opening of the embedding groove 20, the inner wall of the bearing platform 4 is horizontally provided with an annular anti-falling strip 40, when the paraffin block is taken out, the paraffin bearing plate 3 is moved upwards, so that the paraffin block is separated from the embedding groove 20 together with the bearing platform 4, and the paraffin block is fixed when being sliced; a movable pin 7 is clamped at the upper end of the bearing platform 4, a reset spring 70 is arranged at the joint of the movable pin 7 and the bearing platform 4, a pull rod 71 is arranged at the upper end and the lower end of the movable pin 7, a through groove for the left-right sliding of the pull rod 71 is arranged on the bearing platform 4, a clamping groove corresponding to the movable pin 7 is arranged on the through groove 30, the bearing platform 4 and the paraffin bearing plate 3 are clamped through the movable pin 7 and the clamping groove, and the bearing platform 4 and the solidified paraffin blocks can be taken out conveniently;
as shown in fig. 1 and 2, the temperature adjusting device 5 comprises a water cooler 50, a heat preservation pipe 51 and a temperature adjuster 52, the water cooler 50 is arranged at the lower end of the cavity 12, the water cooler 50 is powered by an external power supply, the water cooler 50 adopts a COMP-ILC01 micro temperature-controlled water cooler produced by shenzhen conpkylor science and technology development ltd, the heat preservation pipe 51 is wound on the inner air permeable plate 11, the heat preservation pipe 51 is connected with the water cooler 50, the temperature adjuster 52 is fixedly arranged on the outer wall of the box body 1, and the temperature adjuster 52 is connected with the water cooler 50;
as shown in fig. 1 and 2, the vibration assembly 6 includes a micro-vibration plate 60 and a vibration motor 61, the micro-vibration plate 60 is movably clamped at the lower end inside the embedding seat 2, the vibration motor 61 is fixedly arranged on the outer wall of the box body 1, the vibration motor 61 is connected with the micro-vibration plate 60, the vibration motor 61 provides power for the micro-vibration plate 60, the vibration motor 61 is powered by an external power supply, and the vibration motor 61 adopts a GM16-030 type direct current speed reduction motor produced by shenzhen new fukang science and technology limited; through setting up board 60 and vibrating motor 61 that shakes a little for the paraffin piece after solidifying is compacter, places paraffin and solidifies the in-process and appear hollowing phenomenon, influences the section and observes.
When the paraffin embedding device is used, the water cooling machine 50 and the vibrating motor 61 are respectively connected with an external power supply, then a small amount of paraffin solution is injected into the embedding groove 20, then the dehydrated tissue is placed in the paraffin solution and flattened by medical forceps, the bearing platform 4 is arranged at the lower part of the through groove 30 on the paraffin bearing plate 3, the bearing platform 4 and the paraffin bearing plate 3 are fixed through the movable pin 7 and the clamping groove, and the paraffin solution is injected into the embedding groove 20 through the porous plate 31 until the page is close to the porous plate 31 and then stops; starting a vibration motor 61, vibrating the paraffin solution in the embedding tank 20 by a micro-vibration plate 60, then starting a water cooling machine 50, and adjusting the temperature of a heat preservation pipe 51 by a temperature adjuster 52 to quickly solidify the paraffin solution; after the paraffin solution is completely solidified, adjusting the temperature of the heat preservation pipe 51 to room temperature, covering the sealing cover 13 to store the paraffin block, when tissue slicing needs to be carried out on the paraffin block, taking out the paraffin bearing plate 3 and the bearing platform 4 together with the solidified paraffin block by holding the pulling plate 32 by hand, and removing corner burrs of the solidified paraffin block under the action of the trimming scraper 200; remove movable pin 7, take off paraffin block and cushion cap 4 from bearing wax board 3, with the 4 centre gripping of cushion cap on the slicer to the paraffin block carry out tissue slice can.
Claims (7)
1. A reusable temperature-adjusting paraffin embedding box is characterized by comprising a box body (1), an embedding seat (2), a wax bearing plate (3), a bearing platform (4) and a temperature adjusting device (5); the box body (1) comprises an outer heat-insulation plate (10) and an inner ventilation plate (11), a cavity (12) is formed between the outer heat-insulation plate (10) and the inner ventilation plate (11), a sealing cover (13) is clamped at the upper end of the box body (1), and a lifting handle (130) is arranged on the sealing cover (13); the embedding seat (2) is movably clamped inside the box body (1) and clamped with the box body (1) through a bolt, embedding grooves (20) are arranged on the embedding seat (2) in a matrix mode, the upper ends of the embedding grooves (20) are of a cubic structure, and the lower ends of the embedding grooves (20) are of a chamfered platform structure; the wax bearing plate (3) is movably clamped at the upper end inside the box body (1) and is positioned at the upper end of the embedding seat (2), through grooves (30) are formed in the wax bearing plate (3), the positions and the number of the through grooves (30) correspond to those of the embedding grooves (20), porous plates (31) are horizontally arranged inside the through grooves (30), and lifting plates (32) are arranged at two ends of the upper surface of the wax bearing plate (3); the bearing platform (4) is movably clamped at the lower end of the through groove (30), the bearing platform (4) is hollow, and an opening at the lower end of the bearing platform (4) is embedded with an opening at the upper end of the embedding groove (20); temperature regulation apparatus (5) include cold water machine (50), insulating tube (51) and temperature regulator (52), cold water machine (50) set up cavity (12) lower extreme, and cold water machine (50) are by external power supply, on ventilative board (11) including insulating tube (51) winding set up, and insulating tube (51) are connected with cold water machine (50), temperature regulator (52) are fixed to be set up on the outer wall of box body (1), and temperature regulator (52) are connected with cold water machine (50).
2. The reusable temperature-adjusting paraffin embedding box according to claim 1, further comprising a vibration component (6), wherein the vibration component (6) comprises a micro vibration plate (60) and a vibration motor (61), the micro vibration plate (60) is movably clamped at the lower end inside the embedding seat (2), the vibration motor (61) is fixedly arranged on the outer wall of the box body (1), the vibration motor (61) is connected with the micro vibration plate (60), the vibration motor (61) provides power for the micro vibration plate (60), and the vibration motor (61) is powered by an external power supply.
3. The reusable temperature-adjusting paraffin embedding cassette according to claim 1, wherein the perforated plate (31) is an upwardly convex arc structure, and the aperture of the through hole at the center of the perforated plate (31) is larger than that at the edge of the perforated plate (31).
4. The reusable temperature-adjusting paraffin embedding cassette according to claim 1, wherein the inner wall of the embedding groove (20) is coated with an anti-sticking coating.
5. The reusable temperature-adjusting paraffin embedding box according to claim 1, wherein a movable pin (6) is fastened to the upper end of the bearing platform (4), a reset spring (60) is arranged at the joint of the movable pin (6) and the bearing platform (4), a pull rod (61) is arranged at the upper end and the lower end of the movable pin (6), a through groove for the pull rod (61) to slide left and right is arranged on the bearing platform (4), and a clamping groove corresponding to the movable pin (6) is arranged on the through groove (30).
6. The reusable temperature-adjusting paraffin embedding cassette according to claim 1, characterized in that the inner wall of the bearing platform (4) is horizontally provided with an annular anti-drop strip (40).
7. The reusable temperature-adjustable paraffin embedding cassette according to claim 1, wherein the embedding groove (20) is coated with an anti-sticking coating.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201911046469.8A CN110686961A (en) | 2019-10-30 | 2019-10-30 | But reuse's temperature regulation type paraffin embedding box |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201911046469.8A CN110686961A (en) | 2019-10-30 | 2019-10-30 | But reuse's temperature regulation type paraffin embedding box |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| CN110686961A true CN110686961A (en) | 2020-01-14 |
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Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN201911046469.8A Pending CN110686961A (en) | 2019-10-30 | 2019-10-30 | But reuse's temperature regulation type paraffin embedding box |
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Citations (7)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| MX9503998A (en) * | 1995-09-19 | 1997-03-29 | Hector Marquez Monter | Fast inclusion in paraffin method by microwaves. |
| CN205656048U (en) * | 2016-05-25 | 2016-10-19 | 塔里木大学 | Embedding machine with vibration function |
| CN205981838U (en) * | 2016-08-30 | 2017-02-22 | 练亿香 | Integrated pathological tissue dehydration embedded box |
| CN206536600U (en) * | 2017-03-10 | 2017-10-03 | 甘肃农业大学 | One kind can heat and repair wax spatula |
| CN108051282A (en) * | 2018-01-27 | 2018-05-18 | 塔里木大学 | A kind of semi-automatic embedding machine of paraffin section |
| CN108469368A (en) * | 2018-04-02 | 2018-08-31 | 中国热带农业科学院热带作物品种资源研究所 | A kind of multi-functional paraffin embedding box |
| CN208420501U (en) * | 2018-03-21 | 2019-01-22 | 贵州省人民医院 | A kind of pathology department's private organizations embedding device |
-
2019
- 2019-10-30 CN CN201911046469.8A patent/CN110686961A/en active Pending
Patent Citations (7)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| MX9503998A (en) * | 1995-09-19 | 1997-03-29 | Hector Marquez Monter | Fast inclusion in paraffin method by microwaves. |
| CN205656048U (en) * | 2016-05-25 | 2016-10-19 | 塔里木大学 | Embedding machine with vibration function |
| CN205981838U (en) * | 2016-08-30 | 2017-02-22 | 练亿香 | Integrated pathological tissue dehydration embedded box |
| CN206536600U (en) * | 2017-03-10 | 2017-10-03 | 甘肃农业大学 | One kind can heat and repair wax spatula |
| CN108051282A (en) * | 2018-01-27 | 2018-05-18 | 塔里木大学 | A kind of semi-automatic embedding machine of paraffin section |
| CN208420501U (en) * | 2018-03-21 | 2019-01-22 | 贵州省人民医院 | A kind of pathology department's private organizations embedding device |
| CN108469368A (en) * | 2018-04-02 | 2018-08-31 | 中国热带农业科学院热带作物品种资源研究所 | A kind of multi-functional paraffin embedding box |
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